Casein Hydrolysate Acid Research Articles

Somatic embryo formation, plantlet vigorous and greenhouse preliminary test of garlic bulblet obtained through somatic embryogenesis

Garlic production in Indonesia is still low due to limited availability of seedling. Approximately 10% of garlic yield are used for the next cultivation. Therefore about 90 % of garlic consumption are still imported from China. In addition to conventional seed production, alternative routes are needed, such as through somatic embryogenesis. Research consists of three activities, namely 1) induction and multiplication of callus, 2) embryo somatic and plantlet formation and 3) preliminary test of bulblet in vitro in the greenhouse. Parameters observed in the laboratory activities were time of callus emergence, callus weight and morphology, time of somatic embryo emergence, embryo diameter and weight per bottle, plantlet number and vigorous and bulblet number per bottle, while in the greenhouse, parameter observed were survival of bulblet number, plant height, leaf number per plant, and clove number and weight. The result showed that application of 2,4-D 3 mg/l with casein hydrolysate acid was suitable for callus induction of garlic var. Lumbu Kuning, while BAP 2 mg/l was the most suitable formula for callus multiplication, the addition of paclobutrazol 1 ppm and sucrose 20% in MS medium was the most suitable treatment for plantlet vigorous, while the bulblet formation could be developed by using jasmonic acid with sucrose of 12%. The best medium for growjng media of in vitro bulblet in the greenhouse was the combination of sandy soil, cocopeat and manure.

Somatic embryos cultures of Vitis amurensis Rupr. in air-lift bioreactors for the production of biomass and resveratrol

Resveratrol are the most important bioactive compounds found in Vitis amurensis. In this study, a somatic embryo induction system for V. amurensis was established in air-lift bioreactors for the production of biomass and resveratrol. The somatic embryos biomass growth was low on solid medium (69.60 g L−1) compared to in liquid medium in bioreactor (329.45 g L−1). Bioreactor cultures were found to be superior compared with solid medium culture not only in terms of biomass but also resveratrol productivity. Various culture parameters, including culture method, inoculum density, carbon source, and organic compounds were optimized. An inoculum density of 20 g L−1 embryogenic calli was found suitable for the accumulation of biomass and resveratrol production, whereas 10 g L−1 embryogenic calli increased the amount of resveratrol per fresh weight in somatic embryos. For bioreactor culturing, sucrose was an optimum carbon source and 500 mg L–1 casein hydrolysate acid was conducive to the biomass and resveratrol production. This result indicates that an efficient protocol for the large-scale production of resveratrol can be achieved by bioreactor culturing of V. amurensis somatic embryos and can be used as a source of medicinal raw materials.