Cases Of Laryngeal Carcinoma Research Articles

Correlation of Preoperative Radiological Factors with Histological Involvement of Thyroid Gland in Laryngeal Carcinomas.

Total laryngectomy is the primary treatment for locally advanced laryngeal carcinomas. However, routine thyroid removal (total or hemithyroidectomy) during this procedure is controversial, as the incidence of thyroid gland involvement varies and may lead to lifelong thyroid supplementation, increasing postoperative morbidity. The lack of a consensus on managing the thyroid gland in laryngeal carcinoma cases necessitates improved evaluation techniques, with radiology playing a crucial role in this aspect. Understanding the correlation between radiological factors and histopathological involvement of the thyroid gland can aid in formulating appropriate management strategies during total laryngectomy. To study the correlation of preoperative radiological factors with histopathological involvement of thyroid gland in laryngeal carcinomas. This was a retrospective study which included 57 patients who underwent total laryngectomy for squamous cell carcinoma of larynx. The pre-operative CT findings such as involvement of thyroid cartilage, cricoid cartilage, paraglottic space, anterior commissure, subglottis and thyroid gland along with transglottic extension of tumor were correlated with post-operative histopathological thyroid gland involvement.Cricoid cartilage erosion and thyroid gland involvement in CT scans individually exhibited positive likelihood ratios of 2.58 and 3.23, respectively, demonstrating a reasonable agreement with histopathological findings. The specificity of cricoid cartilage and thyroid gland involvement was also higher with values of 76.4% and 81%, respectively. Moreover, combining thyroid and cricoid cartilage erosion in CT scans as a predictive parameter for thyroid gland involvement resulted in a better likelihood ratio of 8.23 and a fair agreement with histopathological findings. We conclude that cricoid cartilage erosion and thyroid gland involvement in pre-operative CECT can be taken as a preoperative indicator for intraoperative decision on thyroidectomy.

The Follicular Dendritic Cells and HPV 18 Interrelation in Head and Neck Squamous Cell Carcinomas of the Larynx.

Background and Objectives: Even if they are cells of controversial origin (mesenchymal, perivascular, or fibroblastic), follicular dendritic cells (FDC) are present in all organs. The aim of this study was to establish the FDC expression pattern and its interrelation with HPV 18 expression in laryngeal squamous cell carcinoma (LSCC). Materials and Methods: Fifty-six cases of LSCC were evaluated by simple and double immunostaining. The following score was used: 0 (negative or few positive cells), 1 (10-30% of positive cells), 2 (30-50% of cells), and 3 (over 50% of cells). Results: The expression of CD 21-positive cells with dendritic morphology (CDM) was noticed in the intratumoral area of conventional (well and poorly differentiated types and HPV 18 positive cases with a value of 2 for the score) and papillary types (HPV-18 negative cases with a score of 1). The highest value of 2 for the score of CDM in HPV-18 positive cases was found in the peritumoral area of well- and poorly-differentiated conventional LSCCs. A significant correlation was found between scores of CDM from the intratumoral area and those of the peritumoral area (p = 0.001), between CDM and non-dendritic morphology cells (NDM) of the intratumoral area (p = 0.001), and between HPV-18 status and peritumoral NDM cells (p = 0.044). Conclusions: The FDC and NDM cell score values of intratumoral and peritumoral areas may represent important parameters of LSCCs. This may contribute to a better stratification of laryngeal carcinoma cases and the individualized selection of clinical treatment protocols.

Effect of 0.35 T and 1.5 T magnetic fields on superficial dose in MR-guided radiotherapy of laryngeal cancer

BackgroundTreatment of head and neck cancer on linear accelerators with on-board magnetic resonance imaging (MR-linac) might be beneficial to reduce side effects and increase accuracy. For many head and neck cancer patients, dose coverage of the often superficially located planning target volumes (PTVs) is required. This study examines the impact of the electron return effect (ERE) on the surface dose in MR-guided radiotherapy (MRgRT) compared to conventional radiotherapy. Materials and methodsFor this bicentric dosimetric study, 14 cases of laryngeal carcinomas with PTVs reaching up to the skin surface were included. For each patient, five different plans were compared, two VMAT plans (with and without a 5 mm bolus) and three IMRT MRgRT plans (0.35 T, 1.5 T and 0 T, each without bolus). Dose distributions were also validated with film measurements. ResultsA similar coverage on the most superficial 3–5 mm of the PTV was achieved in the VMAT plans with bolus and the MRgRT plans for both 0.35 T and 1.5 T. However, coverage on this region was usually not achieved for VMAT without bolus and the 0 T plans. The film measurements on phantoms confirmed the results with the relative error never exceeding the calculated differences between the plans. ConclusionThe present study could demonstrate that the ERE for both commercially available MR-linac variants provides sufficient coverage of the superficial tissue layers in MRgRT-plans for laryngeal carcinoma.

Prognostic Significance of a Model Based on Acetaldehyde Dehydrogenase 2 Genetic Polymorphisms in Laryngeal Carcinoma.

Because of the high costs associated with early-stage laryngeal carcinoma diagnosis and prognosis prediction, this study attempts to find valuable targets to establish a novel predictive model by focusing on the aldehyde dehydrogenase 2 (ALDH2)genotype and other peripheral blood markers. Retrospective study. Tertiary comprehensive hospital. From January 2011 to January 2021, 362 cases of laryngeal carcinoma were included and divided into 2 groups in this retrospective analysis. Information on medical history, alcohol, and tobacco consumption habits, ALDH2 genotypes, and other peripheral blood markers was collected. Endpoints of the current study included disease-free survival and overall survival. A nomogram model for overall survival was established and evaluated using receiver operating characteristic (ROC) curves. A total of 236 patients were included in the training cohort, and the other 126 were included in the validation cohort. The median follow-up of the patients was 9.6 years (interquartile range: 7.5-12.5 years). Peripheral fibrinogen, hemoglobin, and ALDH2 genotypes were significantly associated with an increase in laryngeal carcinoma mortality rate on Kaplan-Meier curves. The ROC curve showed that the effectiveness of overall survival prediction by the nomogram model was better than that of traditional clinical staging. A prognostic nomogram of laryngeal carcinoma patients involving ALDH2 and peripheral blood markers and T and N stages was constructed and validated.

Ki-67, p-53, E-Cadherin, and β-Catenin expression of advanced glotto-subglottic and supraglottic larynx carcinomas.

Identifying tumor markers that can be used to determine the biological behavior of tumors and predicting their prognosis may be helpful in choosing treatment strategies. Besides the differences in the embryological and histological anatomy of the larynx in this regard, the possibility of molecular causes that can explain the different clinical behaviors has always been a question for the scientific world. In this study, we aimed to investigate whether there were any immunohistochemically molecular differences among laryngeal carcinoma cases originating from two different anatomical regions of the larynx. The study group consisted of 43 patients. The rate of supraglottic cancers was 41.8%, while the rest had glotto-subglottic tumors. Ki67, β-catenin, E-cadherin, and p53 were examined in pathology preparations obtained by laryngectomy surgeries. The data obtained were analyzed by comparing factors that may affect the prognosis of the disease and between tumors originating from the two different anatomical regions. We did not see any statistically significant difference between groups for stage and grade of tumor, tumor recurrence rate, or lymphovascular or perineural invasion rated in terms of the investigated markers. In addition, there was no statistically significant difference between the two distinct groups in survival analysis. With these results, our study differs from some studies in the literature, and we think that this difference could be because the cases in our study consisted of advanced stage tumors and the groups investigated had similar survival rates.

Detection of speech pathology based on parameters of analysis of dysphonia in speech and voice

Objective:To analysis speech pathology based on dysphonia in speech and voice（ADSV）. Methods:The acoustic signals of continuous vowels and continuous speech of one-hundred and thirteen individuals were collected, including 93 vocal cord polyps cases, 20 glottis laryngeal carcinoma cases and 47 volunteers without speech sound disorders. Cepstral peak prominence（CPP）, CPP standard deviation（CPP SD）, L/H spectral ratio（L/H ratio）, L/H ratio standard deviation（L/H ratio SD） and cepstral/spectral index of dysphonia（CSID） were analyzed by ADSV to explore the role of these parameters in the recognition of speech pathology. Results:In the acoustic signal of continuous vowels, CPP and L/H ratio in normal group were higher than those in pathological voice group（P<0.001）, while CPP SD and CSID were lower than those in pathological voice group（P<0.001）, CPP and CSID areas under ROC curve were 0.95 and 0.99, respectively, which were important acoustic parameters for diagnosing pathological voice. In continuous speech acoustic signals, CPP, CPP SD and L/H ratio in the normal group were all higher than those in the speech disorders group（P<0.001）, and the area under the curve of CPP SD was 0.90, which showed high accuracy in diagnosing pathological voice. The ADSV voice analysis parameters CPP, CPP SD, CSID, and L/H ratio also showed significant differences between the vocal cord polyp group and the glottic laryngeal cancer group. The results of the discriminant analysis model show that the use of ADSV voice parameters can distinguish vocal cord polyps and laryngeal cancers. Conclusion:The ADSV voice analysis parameters can not only distinguish the voice signals of the normal group and the pathological group, but also distinguish different types of pathological voices. It has high sensitivity and specificity in diagnosing pathological voices.

Clinical application of supraclavicular fasciocutaneous island flap in the repair of tracheal defects

Objective: To explore the clinical application of supraclavicular fasciocutaneous island flap (SIF) in the repair of tracheal defect. Methods: From May 2016 to March 2021, the clinical data of 10 patients (8 males,2 females,aged 27-73 years old) were retrospectively analyzed who underwent repair surgery with SIF for trachea defects after resection of cervical or thoracic tumors, including 2 cases of laryngotracheal adenoid cystic carcinoma, 2 cases of laryngeal carcinoma, 3 cases of esophageal carcinoma, 2 cases of thyroid carcinoma and one case of parathyroid carcinoma. All of the primary tumors were at T4. The outcomes of 10 cases with tracheal defect repaired by SIF were evaluated. Results: The areas of the SIF were (3-7) cm × (6-10) cm, the thicknesses of the flaps were 8-11 mm, and the lengths of the pedicles were 10-15 cm. The blood supply of the SIF came from the transverse carotid artery. The skin defects of the donor areas of the shoulders were directly closed. After 1-60 months of follow-up, all the flaps survived. The flaps, tracheas as well as shoulder wounds healed well. Conclusion: The SIF is suitable for the repair of tracheal defects. It has perfect thickness compatible with the trachea. The technique is simple and microsurgical technique is not needed, with a good application prospect.

Cardiac Metastasis in a Living Patient With Laryngeal Squamous Cell Carcinoma

Metastatic cardiac tumors are mainly diagnosed postmortem, while cardiac metastases of laryngeal cancer are exceedingly rare. We report a case of laryngeal carcinoma with subsequent metastatic disease to the heart diagnosed nine months after surgical resection of laryngeal cancer. Additionally, we attempted to summarize published case reports of laryngeal cancer with cardiac metastasis. Retrospective chart review and literature search via PubMed and Google Scholar were performed. Twenty cases of laryngeal squamous cell carcinoma (SCC) with cardiac metastatic tumors were identified. We described demographics, typical features seen on diagnostic studies, and analyzed current literature on incidence, diagnostic studies, treatment options, and prognosis of secondary cardiac tumors. More data are needed to decide on the optimal treatment strategy for metastatic cardiac disease.

Clinical-pathological characterization of laryngeal cancer in the Mexican population

Cancer of the larynx occupies the first frequency site of cancer of the upper digestive tract and represents 42% of malignant neoplasms of the upper aero-digestive tract. It is a potentially curable entity if it is diagnosed in early stages. Characterize the clinical pathological features of patients with laryngeal cancer, which can serve as a guide for diagnostic suspicion in primary and secondary care. Descriptive study. All the records of patients with an initial diagnosis of primary laryngeal carcinoma undergoing direct micro-laryngoscopy plus biopsy from January 1, 2014 to December 31, 2018 were analyzed. 173 new cases of laryngeal carcinoma were diagnosed, the male sex predominated (86%). With an average age of presentation of 65 ± 11 years and a history of smoking in 78.6% (n 136). Dysphonia was the initial symptom in 81% (140) of the patients. The profile of the patient with laryngeal cancer corresponds to a patient older than 60 years, male, a history of smoking and a history of dysphonia greater than 30 days. Clinically, we will find an epithelial epidermoid moderately differentiated tumor limited to the glottis.

Surgical emphysema as an early sign for pharyngocutaneous fistula following total laryngectomy: a case report

BackgroundPharyngocutaneous fistula is the most common and troublesome complication after total laryngectomy. Many factors may be used in laryngectomy patients for the early diagnosis of pharyngocutaneous fistulae such as skin flap edema, neck erythema, salivary leak, postoperative barium swallow, and wound amylase level. Its diagnosis is mainly standing on a clinical basis.Case presentationThis report described two cases of laryngeal carcinoma, which presented with a history of persistent hoarseness of voice and mild stridor and were diagnosed with a battery of clinical investigations and managed successfully with total laryngectomy and lateral neck dissection. Both patients suffered from postoperative surgical emphysema before developing pharyngocutaneous fistula.ConclusionSurgical emphysema can precede the occurrence of pharyngocutaneous fistula after total laryngectomy. Further studies are needed to confirm this finding.

Diagnosis and treatment of gastric cancer with immediate double primary carcinoma

Objective: To investigate the clinical characteristics, treatment and prognosis of gastric cancer complicated with immediate double primary cancer. Methods: The clinical data of patients who met the diagnostic criteria of gastric cancer with immediate double primary cancer from January 2016 to June 2019 were analyzed retrospectively. Results: There were 29 cases of gastric cancer with immediate double primary carcinoma, accounting for 1.7% of the 1 741 patients with gastric cancer in the same period. Of these, 17 (58.6%) were more than 70 years of age. 25 cases (86.2%) were male. The postoperative pathological staging was mainly for early gastric cancer (63.2%). Colorectal cancer accounted for 8 cases (27.6%), followed by esophageal cancer in 7 cases (24.1%). The others included 6 cases of lung cancer (20.7%), 2 cases of pancreatic cancer (6.9%), 2 cases of prostate cancer (6.9%), 1 case of non-Hodgkin's lymphoma (3.4%), 1 case of ampullary tumor (3.4%), 1 case of bile duct carcinoma (3.4%) and 1 case of laryngeal carcinoma (3.4%). The prognosis of the surgical treatment group was significantly better than that of the non-surgical treatment group (median survival time: 21.0 months vs 13.0 months, P=0.014). Conclusion: Gastric cancer complicated with immediate double primary cancer mostly occurs in elderly men and early gastric cancer patients. In the complicated tumor, colorectal cancer is the main cause, so we should pay attention to the screening of digestive system. Radical surgery should be performed as far as possible for each primary tumor.

Isolated epiglottic tuberculosis: presented as severe respiratory distress

&lt;p class="abstract"&gt;Tuberculosis is a systemic disease and it can involve any organ of the body. Laryngeal tuberculosis, caused by mycobacterium tuberculosis is quite common in our ENT practice but it is usually a complication of pulmonary tuberculosis, and most patients with laryngeal tuberculosis have coexisting active pulmonary tuberculosis. Most of the times the area of involvement in this secondary laryngeal tuberculosis is posterior part of the larynx. We are reporting a case of isolated primary epiglottic tuberculosis, which is a rare entity, who presented with severe respiratory distress and inspiratory stridor. It was initially suspected and misdiagnosed as a case of laryngeal carcinoma because of common laryngoscopic findings of diffuse edema, deformed laryngeal cartilages and ulcerations in both of these conditions but on further evaluation it was diagnosed as primary epiglottic tuberculosis with no pulmonary involvement. After making the correct diagnosis from histopathological examination, the patient was managed successfully by anti tubercular treatment.&lt;/p&gt;

Effect of vertical anterior laryngectomy with pedicled sternohyoid flap for vocal cord reconstruction and glottal morphology

Objective:To explore the effect of vocal cord reconstruction with sternohyoid muscle flap pedicled with vertical anterior laryngectomy. Method:The clinical data of 43 cases of laryngeal carcinoma were analyzed retrospectively. According to whether the vocal cords were reconstructed, they were divided into vocal cord reconstruction group and non reconstruction group. Among them, 20 patients in the reconstruction group were reconstructed with pedicled sternohyoid muscle flap and 23 patients in non-reconstruction group. In the non reconstruction group, the external membrane of thyroid cartilage was used to repair the wounds. Postoperative respiratory function, swallowing function, pronunciation function, postoperative prognosis, complications, and recurrence rate were compared between the two groups. Reconstruction of glottis after vocal cord reconstruction was evaluated by electronic laryngoscope and CT scan. Result:①Patients in both groups survived during the follow-up period. One patient in the non-reconstructed group had recurrence, and the reconstituted group had no relapse, 3 cases with complications occurred in the reconstruction group, including 2 cases with granulation tissue in the glottic area, 1 case with laryngeal fistula, and 2 cases with aspiration pneumonia were found in the non-reconstruction group. ②1 year postoperative tracheal cannula removal rate, gastric tube removal and pronunciation quality: the tracheal cannula removal rate was 100% in the two groups after surgery; the gastric tube removal time in the reconstruction group was（13.2±2.8） days, and （16.6±5.3） days in the non-reconstruction group （P<0.05）; reconstruction group had good pronunciation in 10 cases, moderate in 6 cases, and poor in 4 cases. Non-reconstructed group had good pronunciation in 4 cases, medium in 14 cases, and poor in 5 cases. Those with moderate or higher were compared no significant difference （P>0.05）, and those with good pronunciation were statistically different （P<0.05）. ③The transverse and anteroposterior diameter in reconstruction group was similar with the normal people （P>0.05）; however, the transverse and anteroposterior diameter in the non-reconstructed group was significantly different with that of the normal people （P>0.05）, the transverse diameter of the reconstructed group and the non-reconstructed group were compared with no significant difference （P>0.05）, but there was significant difference in the anteroposterior diameter between the two groups （P<0.05）. The area in both groups were different with the normal people （P<0.05）; ④Glottic area morphology: the two groups of patients showed different degrees of swelling in the arytenoid cartilage area, the shape of the glottic region in the reconstructed group was approximately triangular, and the glottic morphology in the non-reconstructed group was approximately circular. Conclusion:After vocal cord reconstruction, there were increased rate of tracheal cannula extubation, well covered wallowing and phonation function, and the quality of life of patients was improved.

Prevalence and Detection of Sexually Transmitted Cases of Laryngeal Carcinoma.

Human papillomavirus (HPV) is among the reported etiologies of laryngeal squamous cell carcinoma (LSCC). Understanding the impact of HPV on LSCC may help reduce its incidence. This study investigates the association between HPV and LSCC as well as the roles for different immunohistochemical stains in HPV detection. A total of fifty-two formalin-fixed, paraffin-embedded tissue samples of LSCC, diagnosed between 2005 and 2015, were obtained from the archives of the Pathology Department. The samples were stained and processed to evaluate the relationship of HPV to LSCC. Patients had a mean age of 65.02 ± 14.341 years. By polymerase chain reaction (PCR), high-risk strains of HPV were detected in 15.4% of tissue samples. HPV-16 was found in 75% of the positive samples for an overall prevalence of 13.5%. P16 immunostaining was positive in 15.4% of cases while cyclin B and cyclin E were positive in 65.4% and 76.9%, respectively. Over half of the cases were histologically graded as moderately-differentiated, 28.8% as well-differentiated, and 15.4% as poorly-differentiated. The prevalence of HPV-positive LSCC was lower than previously stated in the literature. HPV-16 was the most commonly detected subtype, in concordance with the findings of multiple other studies. HPV-positive LSCC trended with higher histologic grade. P16 and cyclin E immunohistochemical stains were of limited use in identifying HPV in LSCC. In contrast, cyclin B had a high sensitivity which could be used to rule out HPV in LSCC.

Topographical Distribution of Carcinoma Larynx

The larynx is the most common site for primary tumour of the head and neck region. It represents world wide approximately 1-2% of all cancers. A cross sectional study of 154 cases of laryngeal carcinoma was carried out in the in-patient department of Bangabandhu Sheikh Mujib Medical University and Dhaka Medical College during the period of two years with an aim to find out the frequency of site and subsite involvement of carcinoma of the larynx. The age range is 33-80 years and the over all male to female ratio is 29.8:1. Smoking and chewing habit are the most important associated factors. Majority of growths are found to be exophytic and most of the patients present at advanced stage. The commonest site of laryngeal carcinoma is supraglottic region and occurrence of supraglottic carcinoma than glottic carcinoma is statistically highly significant.&#x0D; Bangladesh J Otorhinolaryngol; April 2018; 24(1): 56-61

Pattern of Nodal Metastasis in Laryngeal Carcinoma

Aims: To find out the occurrence of lymph node metastasis, level of neck node involvement and the pattern of clinical presentation of patients suffering from laryngeal carcinoma.&#x0D; Methods: A cross-sectional study on 90 cases of laryngeal carcinoma was carried out in the inpatient department of Otolaryngology- Head and neck surgery of Dhaka Medical College Hospital, Dhaka during a period of two years. Smoking and chewing betel leaf and tobacco are the most important predisposing factors.&#x0D; Results: Most of the cases presented with more than one symptom. Change of voice was the commonest symptom. Cervical lymphadenopathy are significantly associated with supraglottic carcinoma. Level IV and level III is the commonest level of the lymph node involvement in laryngeal carcinoma.&#x0D; Bangladesh J Otorhinolaryngol; October 2017; 23(2): 109-114

FOXD2-AS1 is corelated with clinicopathological parameter of laryngeal carcinoma and promote cancer cell proliferation

Objective: To investigate the expression and clinical significance of long non-coding RNA FOXD2-AS1 in laryngeal squamous cell carcinoma and its effect on cancer cell proliferation. Method: Real-time quantitative polymerase chain reaction(RT-qPCR) was used to detect the expression of FOXD2-AS1 in 85 cases of laryngeal carcinoma. One-way ANOVA was used to determine relationships between its expression and clinicopathological parameters of laryngeal carcinoma. The prognostic significance of FOXD2-AS1 in head and neck cancer was explored using bioinformatics technology. SiRNA was used to interfere the expression of FOXD2-AS1 in TU686 laryngeal carcinoma cells. MTT and clonal formation assay were used to investigate the biological effect of FOXD2-AS1. MicroRNA binding to FOXD2-AS1 was investigated using double luciferase assay. Result: The expression of FOXD2-AS1 in laryngeal cancer tissues was significantly higher than that in normal tissues(t=10.012, P<0.05), and was associated with T staging of laryngeal cancer(χ=6.41, P=0.016). There were no relationships between FOXD2-AS1 expression and age, sex, smoking history, primary site of tumor and lymph node metastasis(N staging). Survival analysis of head and neck tumors in the TCGA database using GEPIA showed poor prognosis in patients with high FOXD2-AS1 expression(P=0.048). Suppressing FOXD2-AS1 via siRNA in TU686 cells decreased clonal formation ability(t=8.053, P<0.05) and MTT assay confirmed that interference of FOXD2-AS1 down regulated proliferation activity of TU686 cells(t=9.337, P<0.05). Double luciferase assay showed that FOXD2-AS1 could directly bind to miR-206, thus inhibiting the expression of miR-206. Further MTT assay indicated that inhibiting miR-206 attenuated the suppressing effect of si-FOXD2-AS1 on the proliferation of TU686 cells. Conclusion: FOXD2-AS1 is corelated with clinicopathological parameter of laryngeal squamous cell carcinoma and promotes cancer cell proliferation through targeting miR-206.

Matrix metalloproteinases (MMPs) and inhibitor of metalloproteinases (TIMP) mRNA and protein expression in laryngeal squamous cell carcinoma.

IntroductionThe aim of the study was to investigate the mRNA expression and protein levels of matrix metalloproteinases (MMP) 2 (MMP-2), 9 (MMP-9), 7 (MMP-7) and their tissue inhibitor TIMP-2 in patients with laryngeal squamous cell carcinoma and control subjects and additionally to evaluate a possible correlation with clinicopathological features.Material and methodsThe expression levels of MMP-2, MMP-9, MMP-7, and TIMP-2 mRNA were detected by the real-time quantitative real time polymerase chain reaction method in 96 cases of laryngeal carcinoma vs. non-tumor tissue. The blood serum levels of MMP-2, MMP-9, MMP-7, and TIMP-2 in patients with laryngeal cancer and 100 healthy subjects were measured using the enzyme-linked immunosorbent assay (ELISA) method.ResultsThe present study demonstrated that MMP-2, MMP-7, MMP-9 and TIMP-2 mRNA expression levels in carcinoma tissue vs. non-tumor tissue and protein levels in the preoperative serum vs. those obtained in healthy controls were statistically significantly higher than in the healthy controls (p = 0.001). The only significant correlation between mRNA or concentration of measured MMPs and TIMP and the clinicopathological features was found for TIMP-2 protein and for patients with lymph node metastasis. Serum levels of TIMP-2 were higher in cases with lymph node metastasis than in those without lymph node metastasis (p < 0.05).ConclusionsThe results may suggest that MMPs and TIMP-2 are associated with laryngeal tumorigenesis, but we did not find any distinct correlation between the clinicopathological features of laryngeal squamous cell carcinoma patients and expression levels of MMPs and TIMP. The results suggest that the measurement of serum MMP-2, MMP-7, MMP-9 and TIMP-2 concentration might be helpful to diagnose laryngeal squamous cell carcinoma.

A rare case of laryngeal carcinoma in pregnancy

Cancer in pregnancy is uncommon. The ethical problem of managing head and neck cancer in pregnancy has always been a common issue to ensure the mother and fetal well-being. Choices for the diagnostic and treatment modalities are crucial to ensure optimal outcome for the mother as well as to preserve fetal vitality. Best clinical decision for the pregnant patient should be made through a multidisciplinary approach. We are reporting a case of a 24-year-old female patient who presented with stridor owing to supraglottic squamous cell carcinoma at 13 weeks of gestation. She underwent tracheostomy as an emergency procedure and later had tumor excision using carbon dioxide laser at 15 weeks of gestation. She was subsequently decannulated off tracheostomy and later underwent normal vaginal delivery at term and had no recurrence within 2-year follow-up.

MicroRNA‑195 inhibits cell proliferation, migration and invasion in laryngeal squamous cell carcinoma by targeting ROCK1.

Laryngeal carcinoma is the second most common malignancy of the head and neck cancers. The most common type of laryngeal carcinoma comprises laryngeal squamous cell carcinoma (LSCC), which accounts for ~95% of laryngeal carcinoma cases. Despite great progress in diagnostic and therapeutic techniques over the last few decades, the prognosis for patients with LSCC remains poor. A number of studies reported that various miRNAs are dysregulated in LSCC and serve critical roles in LSCC tumorigenesis and tumor development. The present study aimed to evaluate the expression level of microRNA (miR)‑195 and its possible roles in LSCC. Briefly, miR‑195 was downregulated in LSCC tissues and cell lines. In addition, low miR‑195 expression was significantly correlated with lymph node metastasis and TNM stage of LSCC patients. Further study has demonstrated that miR‑195 overexpression suppressed cell proliferation, migration and invasion of LSCC. Moreover, rho‑associated kinase 1 (ROCK1) was identified as a direct target gene of miR‑195. Downregulation of ROCK1 exerted similar roles to that of miR‑195 overexpression in LSCC, suggesting ROCK1 was a direct downstream target of miR‑195. These findings elucidated a novel molecular mechanism for the pathogenic mechanism in LSCC carcinogenesis and progression, and may have a potential role in the treatment of patients with LSCC.