Carrageenan-induced Hind Paw Edema Research Articles

In vivo Anti-Inflammatory and Antinociceptive Activity Evaluation of Brassica Rapa Ssp. Chinensis Ethanolic Extract with In Vitro Thrombolytic and Anthelmintic Activity Test

Objective: Brassica rapa ssp. chinensis is a well-known vegetable plant (Family: Brassicaceae) often grown as a type of Chinese cabbage. The present study was designed to explore the thrombolytic, anthelmintic, anti-inflammatory, and antinociceptive activities of ethanolic extract of the Brassica rapa ssp. chinensis leaves. Methods: The in vitro thrombolytic activity of the leaf extract was evaluated by clot analysis using Streptokinase as a standard drug. The in vitro anthelmintic activity was assessed by applying four different concentrations of the plant extract (10 mg/ml, 20 mg/ml, 40 mg/ml, and 60mg/ml) and recording the time of paralysis and death of worms. Two different doses (100mg/kg and 250mg/kg b.w.) were administered to evaluate the anti-inflammatory activity of the carrageenan-induced paw edema model. The antinociceptive activity was evaluated by hot plate test in mice by two different doses (200mg/kg and 400 mg/kg b.w.). Results: The ethanolic leave extract had thrombolytic action, which broke up blood clots that had already formed. The ethanolic leaf extract showed both paralysis and faster worm death at those concentrations in the anthelmintic test. The extract showed significant anti-inflammatory action in pedal edema compared to the control group. The results show that the highest level of anti-inflammatory action against carrageenan-induced hind paw edema may be achieved with an ethanolic extract of Brassica rapa ssp. chinensis (250 mg/kg, p.o.). The proportion of maximum possible effect in the hot plate test was 82.49 percent in the antinociceptive evaluation. Conclusion: The pharmacological test of leaf extract endorsed the clinical uses of Brassica rapa ssp. chinensis in treating thrombosis, intestinal worms, and inflammation. Thus, this plant could be subjected to extensive bioactive compounds to discover novel therapeutic agents. Keywords: Brassica rapa ssp. chinensis, ethanolic extract, thrombolytic activity, anthelmintic activity, anti-inflammatory activity, antinociceptive activity

Formulation and Evaluation of Herbal Emulsion-Based Gel Containing Combined Essential Oils from Melaleuca alternifolia and Citrus hystrix

Aims: This study aimed to investigate the synergistic anti-inflammatory effects of combined essential oils from Melaleuca alternifolia and Citrus hystrix peel. Methodology: An emulsion-based gel containing combined essential oils from Melaleuca alternifolia and Citrus hystrix peel was topically applied to the injected area of the carrageenan-induced hind paw edema mouse model. Diclofenac sodium was used as a positive control. The inhibition percentage of protein denaturation in all mice was assessed and calculated based on paw volume (Pv). Results: The highest inhibition percentage, recorded at the third hour in the carrageenan-induced hind paw edema mouse model, was 44.76%, achieved by the mice treated with combined essential oils. This was followed by diclofenac-treated (37.14%), tea tree essential oil-treated (27.62%), and kaffir lime peel essential oil-treated mice (20.10%). A similar trend was observed after 24 hours of treatment, with the anti-inflammatory activity of combined essential oils-treated mice recorded at 46.74%, followed by diclofenac-treated (35.87%), tea tree essential oil-treated (24.97%) and kaffir lime peel essential oil-treated mice (11.97%). Conclusion: These findings confirm the synergistic anti-inflammatory effects of the combined essential oils-incorporated emulsion-based gel on the carrageenan-induced hind paw edema mouse model.

Antioxidant and Biological Activities of Mahajanaka Mango Pulp Extract in Murine Models

Mahajanaka mango, a hybrid cultivar of Mangifera indica Linn., is a highly nutritional fruit that is popularly consumed in Thailand. It has been used in traditional medicine due to its abundance of phytonutrients. The present study aimed to investigate the chemical compositions and antioxidant activity of Mahajanaka mango pulp extract (MPE) in vitro. Additionally, we examined its biological activities, including the analgesic, anti-inflammatory, gastroprotective, and hepatoprotective effects of MPE, in murine models. MPE exhibited high levels of phenolic compounds, mangiferin, β-carotene, and vitamin C, and it potentially showed antioxidant properties in an ABTS scavenging assay. The animal results have revealed that oral administration of MPE (1000 mg/kg body weight (BW)) significantly decreased acetic acid-induced writhing responses in mice. Interestingly, local applications of MPE at 1 mg/ear ameliorated ethyl phenylpropiolate (EPP)-induced ear edema, while gavage of MPE at 1000 mg/kg BW significantly decreased carrageenan-induced hind paw edema in rats. MPE can also protect against gastric ulcers induced by stress, hydrochloric acid/ethanol, and indomethacin in rats. Indeed, MPE (250 mg/kg BW) markedly lowered the level of serum alanine aminotransferase activity and hepatic lipid accumulation in rats with CCl4- and paracetamol-induced hepatotoxicity. Taken together, the findings suggested that MPE exerts potent antioxidant, analgesic, anti-inflammatory, gastroprotective, and hepatoprotective effects.

Phytochemical characterization of Typha domingensis and the assessment of therapeutic potential using in vitro and in vivo biological activities and in silico studies.

Typha domingensis, a medicinal plant with significant traditional importance for curing various human diseases, has potentially bioactive compounds but was less explored previously. Therefore, this study aims to investigate the therapeutic potential of T. domingensis by evaluating the phytochemical profile through high-performance liquid chromatography (HPLC) techniques and its biological activities (in vitro and in vivo) from the methanolic extract derived from the entire plant (TDME). The secondary metabolite profile of TDME regulated by reverse phase ultra-high-performance liquid chromatography-mass spectrometry (RP-UHPLC-MS) revealed some bioactive compounds by -ve and +ve modes of ionization. The HPLC quantification study showed the precise quantity of polyphenols (p-coumaric acid, 207.47; gallic acid, 96.25; and kaempferol, 95.78μg/g extract). The enzyme inhibition assays revealed the IC50 of TDME as 44.75 ± 0.51, 52.71 ± 0.01, and 67.19 ± 0.68µgmL-1, which were significant compared to their respective standards (indomethacin, 18.03 ± 0.12; quercetin, 4.11 ± 0.01; and thiourea, 8.97 ± 0.11) for lipoxygenase, α-glucosidase, and urease, respectively. Safety was assessed by in vitro hemolysis (4.25% ± 0.16% compared to triton × 100, 93.51% ± 0.36%), which was further confirmed (up to 10g/kg) by an in vivo model of rats. TDME demonstrated significant (p < 0.05) potential in analgesic activity by hot plate and tail immersion tests and anti-inflammatory activity by the carrageenan-induced hind paw edema model. Pain latency decreased significantly, and the anti-inflammatory effect increased in a dose-dependent way. Additionally, in silico molecular docking revealed that 1,3,4,5-tetracaffeoylquinic acid and formononetin 7-O-glucoside-6″-O-malonate possibly contribute to enzyme inhibitory activities due to their higher binding affinities compared to standard inhibitors. An in silico absorption, distribution, metabolism, excretion, and toxicological study also predicted the pharmacokinetics and safety of the chosen compounds identified from TDME. To sum up, it was shown that TDME contains bioactive chemicals and has strong biological activities. The current investigations on T. domingensis could be extended to explore its potential applications in nutraceutical industries and encourage the isolation of novel molecules with anti-inflammatory and analgesic effects.

Nanofibers of Glycyrrhizin/Hydroxypropyl-β-Cyclodextrin Inclusion Complex: Enhanced Solubility Profile and Anti-inflammatory Effect of Glycyrrhizin.

Despite having a wide range of therapeutic advantages, glycyrrhizin (GL) has few commercial applications due to its poor aqueous solubility. In this study, we combined the benefits of hydroxypropyl β-cyclodextrin (HP-βCD) supramolecular inclusion complexes and electrospun nanofibers to improve the solubility and therapeutic potential of GL. A molecular inclusion complex containing GL and HP-βCD was prepared by lyophilization at a 1:2 molar ratio. GL and hydroxypropyl β-cyclodextrin inclusion complexes were also incorporated into hyaluronic acid (HA) nanofibers. Prepared NF was analyzed for physical, chemical, thermal, and pharmaceutical properties. Additionally, a rat model of carrageenan-induced hind paw edema and macrophage cell lines was used to evaluate the anti-inflammatory activity of GL-HP-βCD NF. The DSC and XRD analyses clearly showed the amorphous state of GL in nanofibers. In comparison to pure GL, GL-HP-βCD NF displayed improved release (46.6 ± 2.16% in 5min) and dissolution profiles (water dissolvability ≤ 6s). Phase solubility results showed a four-fold increase in GL solubility in GL-HP-βCD NF. In vitro experiments on cell lines showed that inflammatory markers like IL-1β, TNF-α, and IL-6 were significantly lower in GL-HP-βCD NF compared to pure GL (p < 0.01 and p < 0.05). According to in vivo results, the prepared nanofiber exhibits a better anti-inflammatory effect than pure GL (63.4% inhibition vs 53.7% inhibition). The findings presented here suggested that GL-HP-βCD NF could serve as a useful strategy for improving the therapeutic effects of GL.

Antiinflammatory, Antinociceptive and Antioxidant activities of aqueous and methanolic stem bark extracts of Fagaropsis hildebrandtii (Engl.) Milne-Redh. (Rutaceae)

Background: Fagaropsis hildebrandtii has been widely used in African traditional medicine to treat various illnesses, particularly those associated with inflammation, pain and oxidative stress. However, experimental data on the anti-inflammatory, anti-nociceptive and antioxidant of the different parts of F. hildebrandtii is still limited. Objectives: The objectives of this study were to determine the phytochemical composition, anti-inflammatory, antinociceptive and antioxidant activities of stem bark extract of F. hildebrandtii from Makueni County, Kenya. Materials and Methods: The stem bark of F. hildebrandtii was extracted by maceration using water and methanol. Standard qualitative methods were then used to determine the presence or absence of various phytochemicals in the extracts. Total phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminium chloride calorimetric methods, respectively. Anti-inflammatory and anti-nociceptive activities of increasing water and methanol extracts doses (2-250mg/Kg) were tested using carrageenan-induced paw edema and acetic acid-induced writhing animal models, respectively in comparison with dexamethasone and aspirin as standard. Antioxidant activities of plant extracts were assayed by 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method, with ascorbic acid as the standard. Data analysis was conducted by two-way ANOVA with appropriate post hoc tests. Statistical significance level was set at p ≤ 0.05. Results: Phenols, flavonoids, alkaloids, saponins, tannins, terpenoids and coumarin were detected in the extracts. However, anthocyanins and anthraquinones were absent in both water and methanolic extracts. Notably, methanol extract had a high total phenolic level compared to water extract (34.590 ± 2.490 mgGAE/g vs. 7.893 ± 1.619 mgGAE/g; p&lt;0.05). Total flavonoid content of F. hildebrandtii methanol extract was also higher in comparison to water extract (132.18 ± 0.26 mgCE/g vs. 42.68 ± 0.93mgCE/g; p&lt;0.05). F. hildebrandtii water and methanol extracts had a radical scavenging activity IC50 of 0.98 µg/ml and 0.987 µg/ml respectively, which was significantly lower than 5.674 µg/ml for ascorbic acid, indicating a higher antioxidant potency. The percentage inhibition of carrageenan-induced hind paw edema by F. hildebrandtii extracts was not significantly different in comparison with 10 mg/Kg of dexamethsone standard over a 5-hour test period (p&gt;0.05). F. hildebrandtii water and methanol extracts exhibited a dose-dependent inhibition of acetic acid-induced writhing in mice, reaching a maximum of 53.1 ± 6.1% and 80.24 ± 12.59%, respectively, at 250 mg/Kg. Notably, the percentage inhibition of acetic acid-induced writhing by 50 mg/kg and 250 mg/kg of F. hildebrandtii methanolic extract was similar to that of aspirin standard at 150 mg/kg (77.75 ± 19.64% vs. 80.24 ± 12.59% vs. 84.99 ± 3.26%, p&gt;0.05, two-way ANOVA). Conclusion: F. hildebrandtii has anti-inflammatory, antinociceptive and antioxidant activities. These findings validate the use of F. hildebrandtii crude extracts among Kenyan ethnic groups in management of diseases associated with inflammation, pain, and oxidative stress.

Anti-Inflammatory, Antinociceptive, Antipyretic, and Gastroprotective Effects of Eurycoma longifolia Jack Ethanolic Extract.

Tongkat ali (Eurycoma longifolia Jack) (ELJ) is a plant in the Simaroubaceae family. Its roots are used in traditional Thai medicine to treat inflammation, pain, and fever; however, the antiulcer abilities of its ethanolic extract have not been studied. This study examined the anti-inflammatory, antinociceptive, antipyretic, and gastroprotective effects of ethanolic ELJ extract in animal models and found that ELJ effectively reduced EPP-induced ear edema in a dose-dependent manner and that a high dose of ELJ inhibited carrageenan-induced hind paw edema formation. In cotton-pellet-induced granuloma formation, a high dose of ELJ suppressed the increases in wet granuloma weight but not dry or transudative weight. In the formalin-induced nociception study, ELJ had a significant dose-dependent inhibitory impact. Additionally, the study found that yeast-induced hyperthermia could be significantly reduced by antipyretic action at the highest dose of ELJ. In all the gastric ulcer models induced by chemical substances or physical activity, ELJ extracts at 150, 300, and 600 mg/kg also effectively prevented gastric ulcer formation. In the pyloric ligation model, however, the effects of ELJ extract on gastric volume, gastric pH, and total acidity were statistically insignificant. These findings support the current widespread use of Eurycoma longifolia Jack in traditional medicine, suggest the plant's medicinal potential for development of phytomedicines with anti-inflammatory, antinociceptive, and antipyretic properties, and support its use in the treatment of gastric ulcers due to its gastroprotective properties.

Fused Triazole-Azepine Hybrids as Potential Non-Steroidal Antiinflammatory Agents

Non-steroidal anti-inflammatory drugs (NSAIDs) are one of the oldest and most widely used groups of drugs nowadays. However, the problem of searching for and creating new NSAIDs remains open, primarily due to the risks owing to their short- and long-term use. In this context, triazole-azepine hybrid molecules are attractive and prospective objects for the rational design of novel potential NSAIDs. In the present work studies of 3-aryl-6,7,8,9-tetrahydro-5H-[1,2,4]triazolo[4,3-a]azepines as potential non-steroidal anti-inflammatory agents are reported. Evaluation of drug-like properties for all tested triazole-azepine hybrids was performed in silico using SwissADME. The screening of analgesic and anti-inflammatory activities was performed in vivo using acid-induced writhing and carrageenin-induced hind paw oedema models in mice. Derivatives with activity levels more potent compared with reference drugs ketorolac and diclofenac sodium were identified. Preliminary SAR was performed based on the screening results.

The Impact of the Recipe from Zanthoxylum leprieurii Bark, Harungara madagascariensis Leaves and Xylopia aethiopica Fruits on Nociception and Inflammation in Wistar Rats

Inflammation has a major role in the pathophysiology of sickle cell disease, in fact when it is recurrent, it contributes more to the obstruction of blood vessels through several biochemical mechanisms by provoking vaso-occlusive crises. In order to mitigate these adverse effects in sickle cell patients, the analgesic and anti-inflammatory potentials of extracts of a combination of organs of three plants were evaluated in this study. The analgesic and anti- inflammatory activities of the aqueous (DZHm) and hydroethanolic (EZHm) extracts (200, 400 and 800 mg/kg) were evaluated in rats as models of acetic acid-induced torsion and Carrageenan-induced hind paw edema, using Diclofenac and Ibuprofen as reference molecules. Oral administration of ZHm extracts at doses of 200, 400 and 800 mg/kg produced anti-inflammatory and analgesic effects. These extracts significantly reduced edema and pain. These effects are time and dose dependent. In terms of analgesic effect, the dose of 800 mg/kg bw generated a high inhibition rate of 84.51% and 73.06% for DZHm and EZHm respectively, while the inhibition percentage of Ibuprofen was 78.3 ± 2.09 at the dose of 100 mg/kg bw. The anti-inflammatory effect was 60, 55% for DZHm, 56.08% for EZHm and 62.91% for Diclofenac at 10 mg/kg Pc. Diclofenac and Ibuprofen used for these two tests generated similar activities with ZHm extracts at the dose of 800 mg/kg Pc. The tested extracts (DZHm and EZHm) have analgesic and peripheral anti-inflammatory potentials justifying the use of this recipe in the traditional environment.

Leaf-buds of Pistacia atlantica: a novel source of bioactive molecules with high anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial properties.

In this study, for the first time, the anti-inflammatory, antioxidant, anti-tyrosinase and antimicrobial property of P. atlantica Desf. subsp. atlantica leaf-bud extracthave been investigated. The anti-inflammatory activity was determined in vivo by reducing carrageenan-induced hind paw edema in mice, while the antiradical function was evaluated using DPPH, total antioxidant capacity (TAC) and reduction power assays. The extract induced a significant reduction of the edema, from 1 to 6h in a dose-dependent manner (150, 200 and 300mg/kg). Histological observations of the inflamed tissues also confirmed this. An effective antioxidant activity of the plant samples was demonstrated, showing an EC50 = 0.183 ± 0.005mg/mL for the DPPH test, a value of 28.776 ± 2.541mg AAE/g for the TAC and an EC50 = 0.136 ± 0.003mg/mL for reducing power. The leaf-bud extract also revealed a good antimicrobial activity against S. aureus and L. monocytogenes (mean diameter of inhibition zones of 13.2 and 17.0mm, respectively), while a slight antifungal effect was observed. The plant preparation was then documented to inhibit tyrosinase activity, with an EC50 value of 0.098 ± 0.00mg/mL in a dose-dependent manner. HPLC-DAD analysis revealed that dimethyl-allyl caffeic acid and rutin were the most abundant molecules. The current data documented that P. atlantica leaf-bud extract has strong biological properties and constitutes a potential source of pharmacological molecules.

Anti-inflammatory Activity of the Phenolic Compounds from Vanda roxburghii R. Br.

Inflammation is implicated in the pathogenesis of several neurodegenerative diseases including Alzheimer’s disease (AD). Vanda roxburghii R. Br. is used as folk medicine in the treatment of various ailments and diseases including inflammation, rheumatoid arthritis and neurological disorders. We reported earlier that the plant possesses anti-inflammatory activity and the activity is high in the chloroform fraction among other fractions tested. Therefore, the present work was designed to isolate the compounds from the chloroform fraction responsible for anti-inflammatory activity. Four compounds namely syringaldehyde (1), vanillin (2), dihydroconiferyl dihydro-p-coumarate and (3) and gigantol (4) were isolated and purified using chromatographic methods. The isolated compounds were identified by analysis of their NMR spectral data. The compounds were evaluated for anti-inflammatory activity using carrageenan-induced hind paw edema model in mice. All the compounds showed significant activity at a dose of 100 mg/kg. Among the compounds, gigantol displayed the highest activity which was comparable to the activity of the standard drug indomethacin. At the same dose the other three compounds syringaldehyde, vanillin and dihydroconiferyl dihydro-p-coumarate exhibited similar activity. These results suggest that the isolated compounds from the chloroform fraction have potential anti-inflammatory activity that might be useful in the prevention and treatment of AD. Bangladesh Pharmaceutical Journal 26(1): 1-6, 2023 (January)

Analgesic and Anti-Inflammatory Effects of 80% Methanol Extract and Solvent Fractions of the Leaves of Vernonia auriculifera Hiern. (Asteraceae).

The leaves of V. auriculiferahas been used traditionally for the treatment of inflammatory disorders, and pain in various parts of Ethiopia. However, to our knowledge, the analgesic and anti-inflammatory activity of the crude extract and solvent fractions has never been experimentally studied. To assess the analgesic and anti-inflammatory activitiesof V. auriculifera leaf extract and solvent fractions in rodent models. Air-dried leaves ofV. auriculiferawere grounded and macerated using 80% methanol. The air-dried, grounded leaves were also successively extracted with ethyl acetate, and methanol. The residue was then macerated in water for 72 hr. The extract's peripheral analgesic activity, as well as the solvent fractions, were determined using an acetic acid-induced writhing test. The hot plate model was used to assess the central analgesic effect. Carrageenan-induced hind paw edema and cotton pellet-induced granuloma models were used to assess the anti-inflammatory effect in rats. The 80% methanol leaf extract and solvent fractions have demonstrated significant (p < 0.05) peripheral and central analgesic activity. Both 80% methanol leaf extract and solvent fractions of V. auriculifera were found to have anti-inflammatory activity in a carrageenan-induced rat paw edema model. In the cotton pellet-induced granuloma model, all concentrations of 80% methanol leaf extract (ME), methanol fraction (MEF), and aqueous fractions (AQF) of V. auriculifera inhibited exudate and granuloma formation. Although all tested doses significantly inhibited granuloma mass formation, only the medium and highest ethyl acetate fraction (EAF) doses significantly inhibited the generation of inflammatory exudate. This study's findings indicate that the solvent fractions and 80% methanol extract of V. auriculifera have analgesic and anti-inflammatory properties. This study's findings not only confirm the plants' traditional claim but also provide clues for further investigation of the active principles of this plant for the development of effective and safe analgesic and anti-inflammatory drugs.

Synthesis and Biological Evaluation of Some New Chalcone Derivatives as Anti-inflammatory Agents.

Aim-The present research work aims to prepare a series of 1-(4-(2-(1H-indol-1-yl)-2-oxoethoxy)phenyl)-3-phenylprop-2-en-1-one derivatives. Method- The major compound was achieved by the reaction of indole with chloroacetylchloride in benzene afforded 2-chloro-1-(indoline-1-yl) ethanone which reacts o- hydroxy acetophenone in presence of acetonitrile to form 2-(4-acetylphenoxy)-1-(1H-indol-1-yl)ethan-1-one then is goes through aldol condensation to give various final derivatives. Results and conclusion- After synthesis of compounds, the synthesized compounds were characterized by checking their solubility, melting point, thin layer chromatography, IR, 1HNMR spectral data and elemental analysis. All of prepared derivatives were evaluated for their anti-inflammatory activity on wistar albino rats by following carrageenan induced Rat Hind Paw Edema model.

In-vivo Wound Healing and Anti-Inflammatory Activity of the Solvent Fraction of Zehneria scabra L.F. Sond (Cucurbitaceae) Leaves.

Wounds continue to be a difficult clinical problem, with early and late consequences causing significant morbidity and death. As a result, proper wound management is critical. In addition to contemporary medicine, medicinal herbs serve an essential role in the treatment of wounds and bacterial infections. Z. scabra is a medicinal plant that has traditionally been used to treat wounds. However, there are no scientific reports on solvent fraction wound healing activities. As a result, the current study presents a scientific assessment of the wound healing ability of the solvent fractions of Z. scabra leaves. The leaves were crushed and macerated three times in 80% methanol. Chloroform, ethyl acetate, and aqueous fractions of simple ointment at 5% w/w and 10 percent w/w strengths were prepared using the fusion technique based on the British Pharmacopoeia. Excision and incision wound models were used to assess the solvent fractions' wound healing activities. The anti-inflammatory efficacy of crude and solvent fractions was tested in mice utilizing a carrageenan-induced hindpaw edema model. In rats, a test dose of 2000 mg/kg of the 10% w/w crude extract ointment was found to be safe. Groups treated with the 5% and 10% ethyl acetate fractions of the extract experienced significant (p<0.05 and p<0.01) wound reduction in the excision wound model. When compared to the negative control, the length of epithelization in groups treated with 10% ethyl acetate fraction and aqueous fractions of Z. scabra was statistically significant (p 0.001). By lowering the amount of carrageenan-induced paw edema, the leaf extract and the chloroform fraction of Z. scabra demonstrated a dose-dependent anti-inflammatory effect. The extract showed remarkable wound healing and anti-inflammatory activity and might be recommended for the treatment of many types of human wounds.

Evaluation of Anti-Inflammatory and Immunomodulatory Activities of Ayapoonaga Chenduram, Seenthil Choornam and their Combination in Wistar Rats

Ayapoonaga chendooram (APNC) and Seenthilchoornam (SC) are two polyherbal Siddha formulations that traditionally have been used as a folklore medicine to treat rheumatism, orchitis, bronchitis, asthma, tuberculosis (TB), and cough. In the present study, an attempt has been made to demonstrate the antiinflammatory and immunomodulatory activities of individual APNC and SC and also in their combination (APNC+SC). The acute anti-inflammatory effect of APNC, SC and their combination was evaluated in carrageenan-induced hind paw edema in wistar rats. Diclofenac sodium was used as reference drug for comparison. The immunomodulatory activity was evaluated in rats by studying the hemagglutination antibody (HA) titer, delayed type hypersensitivity (DTH) reactions and the Ig E levels. The cyclophosphamide is used as an immunosuppressant.Diclofenac Sodium (50mg/kg) significantly inhibited the carrageenan-induced hind paw edema, indicating its strong anti-inflammatory activity. APNC (65 mg/kg b.w.p.o) at 2nd hour and APNC+SC (13+200 mg/kg b.w.) at 3rd hour had shown moderate inhibition of paw edema, indicating their mild-moderate anti-inflammatory activity but statistically, no significant difference was observed (p&gt;0.05). Oral administration of APNC, SC, and APNC + SC for 21 days increases the left paw volume at 1st hour and 2nd hour resulting in hypersensitivity reaction in rats. The agglutination (dilution factor 1:200) was visually observed in APNC 130mg/kg b.w. (moderate agglutination) and in APNC + SC (high agglutination) results in humoral immunity. Mild increased levels of Ig E were observed in APNC (30 mg/kg b.w.), SC (200 mg/kg b.w.) and in APNC + SC (65+1000 mg/kg b.w.). Under the conditions of the present study, single oral administration of APNC, SC and their combination has shown mild anti-inflammatory activity against carrageenan induced paw edema in experimental rats. The immunity has been stimulated by APNC (13mg/kg b.w.) and SC (2000mg/kg b.w.).

Ginger Extract-Loaded Sesame Oil-Based Niosomal Emulgel: Quality by Design to Ameliorate Anti-Inflammatory Activity.

Ginger, a natural plant belonging to the Zingeberaceae family, has been reported to have reasonable anti-inflammatory effects. The current study aimed to examine ginger extract transdermal delivery by generating niosomal vesicles as a promising nano-carrier incorporated into emulgel prepared with sesame oil. Particle size, viscosity, in vitro release, and ex vivo drug penetration experiments were performed on the produced formulations (ginger extract loaded gel, ginger extract loaded emulgel, ginger extract niosomal gel, and ginger extract niosomal emulgel). Carrageenan-induced edema in rat hind paw was employed to estimate the in vivo anti-inflammatory activity. The generated ginger extract formulations showed good viscosity and particle size. The in vitro release of ginger extract from niosomal formulation surpassed other formulations. In addition, the niosomal emulgel formulation showed improved transdermal flux and increased drug permeability through rabbit skin compared to other preparations. Most importantly, carrageenan-induced rat hind paw edema test confirmed the potential anti-inflammatory efficacy of ginger extract niosomal emulgel, compared to other formulations, as manifested by a significant decrease in paw edema with a superior edema inhibition potency. Overall, our findings suggest that incorporating a niosomal formulation within sesame oil-based emulgel might represent a plausible strategy for effective transdermal delivery of anti-inflammatory drugs like ginger extract.

Evaluation of Wound Healing and Anti-Inflammatory Activity of Hydroalcoholic Leaf Extract of Clematis simensis Fresen (Ranunculaceae).

IntroductionClematis simensis is one of the most widely used medicinal plant for the treatment of wound traditionally. However, its claim was not scientifically tested, the current study therefore assessed the anti-inflammatory and wound healing properties of 80% methanol leaf extract of C. simensis.MethodsThe dried and powdered leaf of C. simensis was macerated with 80% methanol. The topical ointment was then made in two concentrations (5% and 10% w/w), and two models, excision and incision, were used to test the extract’s capacity to treat wounds in mice. In addition, anti-inflammatory test was also conducted using carrageenan-induced hind paw edema model in three doses (100, 200, and 400 mg/kg) in rats. The DPPH test was used to determine the extract’s anti-oxidant properties where ascorbic acid was used as standard agent.ResultsWhen wounds were treated with ointments containing 5% and 10% (w/w) extract, the rate of wound contraction, the length of time it took for the epithelium to form, and the strength of the skin to break were all significantly increased (p < 0.05). There was no discernible difference in wound healing activity between the 10% (w/w) and 5% (w/w) extracts. Moreover, they had also similar impact as that of the positive control nitrofurazone in wound healing activity. Compared to the negative control, C. simensis extract considerably (p < 0.01-p < 0.001) reduced inflammation. The extract also demonstrated antioxidant activity with IC50 values of 0.7 mg/mL for the extract and 1.04 mg/mL for ascorbic acid, respectively.ConclusionOverall, it is plausible to draw the conclusion that C. simensis 80% methanol extract possesses wound healing activity, perhaps as a result of its anti-inflammatory and antioxidant effects.

Anti-Inflammatory Activity of Essential Oil from Zingiber ottensii Valeton in Animal Models.

Zingiber ottensii (ZO) Valeton, a local plant in Northern Thailand, has been widely used in traditional medicine. Many studies using in vitro models reveal its pharmacological activities, including the anti-inflammatory activity of ZO essential oil, extracted from ZO rhizomes. However, the scientific report to confirm its anti-inflammatory activity using animal models is still lacking. The present study aimed to evaluate the anti-inflammatory activity and explore the possible mechanisms of action of ZO essential oil in rats. The results revealed that ZO essential oil significantly reduced the ear edema formation induced by ethyl phenylpropiolate. Pre-treatment with ZO essential oil significantly reduced the carrageenan-induced hind paw edema and the severity of inflammation in paw tissue. In addition, pre-treatment with ZO essential oil exhibited decreased COX-2 and pro-inflammatory cytokine TNF-α expression in paw tissue, as well as PGE2 levels in serum. On this basis, our study suggests that ZO essential oil possesses anti-inflammatory activity in animal models. Its possible mechanisms of action may involve the inhibition of TNF-α expression as well as the inhibition of COX-2 and PGE2 production. These findings provide more crucial data of ZO essential oil that may lead to new natural anti-inflammatory product development in the future.

Anti-inflammatory, antinociceptive effects and involvement of opioid receptors in the antinociceptive activity of Eugenia uniflora leaves obtained with water, ethanol, and propylene glycol mixture

Ethnopharmacological relevanceEugenia uniflora (Myrtaceae) is a species native to Brazil and has a traditional use in the treatment of inflammation. Aim of the studyTo evaluate the anti-inflammatory and antinociceptive effects, and the involvement of opioid receptors in the antinociceptive activity of extract and fractions from Eugenia uniflora leaves. Materials and methodsTLC and HPLC were used to characterize the spray-dried extract (SDE) and fractions. In the in vivo assays, Swiss (Mus musculus) mice were used. Carrageenan-induced hind-paw edema and carrageenan-induced peritonitis models were used to determine the anti-inflammatory effect of the extract (50, 100, or 200 mg/kg). Acetic acid-induced writhing, tail-flick, and formalin tests were used to determine the antinociceptive effect of the extract (50, 100, or 200 mg/kg). The aqueous (AqF) and ethyl acetate (EAF) fractions (6.25, 12.5, and 25 mg/kg) were then combined with naloxone to evaluate the involvement of opioid receptors in the antinociceptive activity. ResultsIn this work, the TLC and HPLC analysis evidenced the enrichment of EAF, which higher concentration of gallic acid (5.29 ± 0.0004 %w/w), and ellagic acid (1.28 ± 0.0002 %w/w) and mainly myricitrin (8.64 ± 0.0002 %w/w). The extract decreased the number of total leukocytes and neutrophils in the peritoneal cavity (p < 0.05), at doses of 100 and 200 mg/kg and showed significant inhibition in the increase of paw edema volume (p < 0.05). The treatment per oral route (doses of 50, 100, and 200 mg/kg) significantly reduced the nociceptive response in acetic acid-induced abdominal writhing (p < 0.05). The effect of the extract on the tail-flick test showed a significant increase in latency time of animals treated at doses of 200 and 100 mg/kg (p < 0.05). The extract and ethyl acetate fraction reduced the nociceptive effect in both phases of formalin at all tested doses. The naloxone reversed the antinociceptive effect of EAF, suggesting that opioid receptors are involved in mediating the antinociceptive activity of EAF of E. uniflora in the formalin test. ConclusionThe current study demonstrates the anti-inflammatory and analgesic activities of water: ethanol: propylene glycol spray-dried extract from E. uniflora leaves using in vivo pharmacological models in mice. Our findings suggest that spray-dried extract and ethyl acetate fraction exhibit peripheral and central antinociceptive activity with the involvement of opioid receptors that may be related to the presence of flavonoids, mainly myricitrin.

Tailoring dexamethasone loaded albumin nanoparticles: A full factorial design with enhanced anti-inflammatory activity In vivo

Protein-based nanoparticles (NPs) are biodegradable, biocompatible, and easily amenable to chemical modifications to allow for the incorporation of bioactive compounds. In the current study, we adopted a full factorial design to optimize dexamethasone disodium phosphate (Dex) encapsulation within NPs formed with bovine serum albumin (BSA) and stabilized using polyethylenimine (PEI). The optimized NP size (<200 nm dia.), zeta potential (−23.3 mV), and entrapment efficiency (67.4%) were occurred at 0.652 mg, 0.04, 5%, and 8.3 for solution concentration of Dex, PEI/BSA molar ratio, BSA solution concentration, and solution pH, respectively. Incorporation of Dex resulted in a decrease in alpha helix content of BSA indicating a change in its secondary structure. Dex loaded NPs yielded a bimodal release of Dex over an extended period of time via a quasi-Fickian diffusion mechanism. The in vivo anti-inflammatory activity of BSA/PEI Dex NPs surpassed that of free Dex in carrageenan-induced hind paw edema in rats as evidenced by enhanced suppression of oxidative stress, abrogation of NF-κB-p65 expression, as well as reduced myositis and inflammatory cell infiltration. The extended-release profile of BSA/PEI Dex NPs is crucial for achieving a significantly significant anti-inflammatory activity.