Captive Orangutans Research Articles

Isolation of a CTX-M-55 (ESBL)-Producing Escherichia coli Strain of the Global ST6448 Clone from a Captive Orangutan in the USA.

The purpose of this study was to determine if orangutans (Pongo spp.) living in captivity at a zoo in Wisconsin were colonized with antimicrobial-resistant bacteria and, if found, to identify underlying genetic mechanisms contributing to their resistant phenotypes. We hypothesize that since antimicrobial-resistant bacteria are so prevalent within humans, the animals could also be carriers of such strains given the daily contact between the animals and the zoo staff that care for them. To test this theory, fecal samples from two orangutans were examined for resistant bacteria by inoculation on HardyCHROM™ ESBL and HardyCHROM™ CRE agars. Isolates were identified using MALDI-TOF mass spectrometry and antimicrobial susceptibility testing was performed using a Microscan autoSCAN-4 System. An isolate was selected for additional characterization, including whole genome sequencing (WGS). Using the Type (Strain) Genome Server (TYGS) the bacterium was identified as Escherichia coli. The sequence type identified was (ST/phylogenetic group/β-lactamase): ST6448/B1/CTX-M-55.

Understanding the welfare requirements of a neurologically and physically divergent captive male Sumatran orangutan (Pongo abelii)

Background Understanding the needs of animals with physical and cognitive impairments is essential for zoos, rehabilitation centres, and other captive contexts. This case study describes the atypical physical and cognitive development of Jiwa, an adult male Sumatran orangutan (Pongo abelii), to evaluate how these differences may impact Jiwa and to explore how cognitive enrichment can be tailored to his skill level. Methods Keeper records from Jiwa’s birth (1999) to February 2022 were scrutinised to identify key developmental milestones, changes in body weight, and deciduous dental emergence. These were compared with expected milestones for wild and captive orangutans. In addition, a probe feeder apparatus was gradually tailored to an appropriate skill level between January and March 2022. Results Many of Jiwa’s developmental milestones were consistent with expected orangutan development, although there were noticeable differences in body weight, dental emergence, locomotion, and cognition. The welfare domains most likely to be negatively impacted by these differences were physical health, expression of agency, and mental wellbeing. After the enrichment device was tailored to an appropriate level of challenge, Jiwa showed marked improvement in engagement and ability to use the device. Conclusions This study helps to inform future case studies of atypical great apes by detailing the physical and cognitive development of an individual orangutan. Although Jiwa’s differences may impact his own wellbeing in some areas, he has largely been able to overcome them. However, this case study highlights the challenges involved in tailoring enrichment apparatuses and resilience interventions for atypical individuals.

A new species of Versteria (Cestoda: Taeniidae) parasitizing Neogale vison and Lontra canadensis (Carnivora: Mustelidae) from Western Canada.

Via molecular and morphological analyses, we describe adult specimens of a new species of Versteria (Cestoda: Taeniidae) infecting mink and river otter (Carnivora: Mustelidae) in Western Canada, as well as larval forms from muskrat and mink. These sequences closely matched those reported from adult specimens from Colorado and Oregon, as well as larval infections in humans and a captive orangutan. We describe here a new species from British Columbia and Alberta (Canada), Versteria rafei n. sp., based upon morphological diagnostic characteristics and genetic distance and phylogeny. Versteria rafei n. sp. differs from the three other described species of the genus in the smaller scolex and cirrus sac. It also differs from V. mustelae (Eurasia) and V. cuja (South America) by having an armed cirrus, which is covered in hair-like bristles, and in the shape of its hooks, with a long thorn-like blade, and short or long handle (vs. a short sharply curved blade and no difference in handle size in previously described species). The poorly known V. brachyacantha (Central Africa) also has an armed cirrus and similarly shaped hooks. However, it differs from the new species in the number and size of hooks. Phylogenetic analysis of the cox1 and nad1 mitochondrial regions showed that our specimens clustered with isolates from undescribed adults and larval infections in North America, and separate from V. cuja, confirming them to be a distinct species from the American Clade.

Metagenomic analysis of mycobiome in wild and captivity Sumatran orangutans (Pongo abelii).

This study analyzes the mycobiome in wild and captive Sumatran orangutans. Nine orangutan feces samples from the wild and nine from captivity were divided into three repeats from 11- to 15-year-olds in good health. The Illumina platform for analysis of ITS bioinformatics was used according to the Qiime2 and CCMetagen approaches. Wild Sumatran orangutans include 53% Ascomycota, 38% uncultured fungi, and 4% Basidiomycota. Orangutans in captivity are 57% Ascomycota, 26% uncultured fungi, and 2% Basidiomycota. Based on genus level, uncultured Neurospora (31%), Penicillium (10%), Aspergillus (3%), Fusarium (3%), Candida (2%), Cutaneotrichosporon (2%), and Limonomyces (2%) are found in wild orangutans. The most prevalent genus among captivity orangutans is Aspergillus (32%), followed by fungal sp. (11%), Lasiodiplodia (18%), Devriesia (2%), and Sordariomycetes (2%). According to the Chao1 diversity index and Shannon and Simpson, there was no significant difference between wild and captive Sumatran orangutans. Neurospora is unique to wild Sumatran orangutans, although Aspergillus predominates in captive orangutans. We hypothesize that the gut mycobiome of wild orangutans will resemble that of orangutans in captivity. The excellent range of food sources in the forest does not result in the prevalence of fungi in the typical gut microbiome.

First Study on profiling of gut microbiome in wild and captive Sumatran orangutans (Pongo abelii).

Orangutans are an "umbrella species" for conserving tropical forests in Sumatra and Kalimantan. There are remarkable changes between the gut microbiomes of wild and captive Sumatran orangutans. This study aimed to profile gut microbiota of wild and captive Sumatran orangutans. Nine fecal samples collected from wild orangutans and nine fecal samples collected from captive orangutans were divided into three replicates. Each replicate randomly combined three pieces and were analyzed on the Illumina platform. A bioinformatics study of 16S rRNA according to Qiime2 (Version 2021.4) and microbiome profiling analysis was conducted. The relative abundance of different microbial taxa varied significantly between wild and captive Sumatran orangutans. Among the operational taxonomic units, various proportions of Firmicutes, Proteobacteria, Bacteroidetes, Euryarchaeota, Acidobacteria, Actinobacteria and Verrucomicrobia predominated. Solobacterium was found only in 19% of captive orangutans. Methanobrevibacter was identified to be prevalent among wild orangutans (16%). Analysis of the core microbiome from the combined wild and captive data revealed seven species as cores. According to linear discriminant analysis effect size, Micrococcus luteus, Bacteroidescaccae, Lachnospiraceae bacterium, Ruthenibacterium lactatiformans, Haemophilus haemolyticus, and Chishuiella spp. were microbiome biomarkers in captive orangutans, whereas Roseburia inulinivorans, Collinsella aerofaciens, Oscillibacter spp., and Eubacterium hallii were microbiome biomarkers in wild orangutans. There were differences in the microbiome biomarkers of wild and captive Sumatran orangutans. This study is important for understanding the role of gut bacteria in the health of Sumatran orangutans.

Identification of potentially zoonotic parasites in captive orangutans and semi-captive mandrills: Phylogeny and morphological comparison.

Cysts and trophozoites of vestibuliferid ciliates and larvae of Strongyloides were found in fecal samples from captive orangutans Pongo pygmaeus and P. abelii from Czech and Slovak zoological gardens. As comparative material, ciliates from semi-captive mandrills Mandrillus sphinx from Gabon were included in the study. Phylogenetic analysis of the detected vestibuliferid ciliates using ITS1-5.8s-rRNA-ITS2 and partial 18S ribosomal deoxyribonucleic acid(rDNA) revealed that the ciliates from orangutans are conspecific with Balantioides coli lineage A, while the ciliates from mandrills clustered with Buxtonella-like ciliates from other primates. Morphological examination of the cysts and trophozoites using light microscopy did not reveal differences robust enough to identify the genera of the ciliates. Phylogenetic analysis of detected L1 larvae of Strongyloides using partial cox1 revealed Strongyloides stercoralis clustering within the cox1 lineage A infecting dogs, humans, and other primates. The sequences of 18S rDNA support these results. As both B. coli and S. stercoralis are zoonotic parasites and the conditions in captive and semi-captive settings may facilitate transmission to humans, prophylactic measures should reflect the findings.

Potential resilience treatments for orangutans (Pongo spp.): Lessons from a scoping review of interventions in humans and other animals.

Wild orangutans (Pongo spp.) rescued from human-wildlife conflict must be adequately rehabilitated before being returned to the wild. It is essential that released orangutans are able to cope with stressful challenges such as food scarcity, navigating unfamiliar environments, and regaining independence from human support. Although practical skills are taught to orangutans in rehabilitation centres, post-release survival rates are low. Psychological resilience, or the ability to 'bounce back' from stress, may be a key missing piece of the puzzle. However, there is very little knowledge about species-appropriate interventions which could help captive orangutans increase resilience to stress. This scoping review summarises and critically analyses existing human and non-human animal resilience literature and provides suggestions for the development of interventions for orangutans in rehabilitation. Three scientific databases were searched in 2021 and 2023, resulting in 63 human studies and 266 non-human animal studies. The first section brings together human resilience interventions, identifying common themes and assessing the applicability of human interventions to orangutans in rehabilitation. The second section groups animal interventions into categories of direct stress, separation stress, environmental conditions, social stress, and exercise. In each category, interventions are critically analysed to evaluate their potential for orangutans in rehabilitation. The results show that mild and manageable forms of intervention have the greatest potential benefit with the least amount of risk. The study concludes by emphasising the need for further investigation and experimentation, to develop appropriate interventions and measure their effect on the post-release survival rate of orangutans.

A composite menstrual cycle of captive orangutans, with associated hormonal and behavioral variability.

Knowledge of species-typical reproductive endocrinology profiles is crucial for testing hypotheses pertaining to the evolutionary history, reproductive parameters, and life history of a species, and for managing the well-being of individual animals in human care. Large-scale empirical measurements of ovarian hormones, however, are rare for most primate species, including orangutans. In this study, we used enzyme immunoassays (EIA) to quantify estrogen (estrone conjugates; E1 C) and progesterone (pregnanediol-3-glucuronide; PdG) levels for 98 cycles in 7 cycling zoo-housed female orangutans (10-43 years old). We use a subset of these cycles (N = 44) to create the first composite menstrual cycle for orangutans, which serves as a valuable baseline for future comparative analyses and veterinary considerations. Similar to previous studies, we determined the mean ovarian cycle length of orangutans to be 29.7 days (N = 98 cycles), although we illustrate evidence of both intra- and interindividual variation in ovarian steroid production. Given that this study took place in captivity, we consider how energetic and psychosocial aspects of the zoo environment, such as greater food availability and potential stress, may affect the reproductive physiology and sexual behavior of these females. Furthermore, we discuss the role that age and genetic background may play in producing variability. Finally, we test whether ovarian hormone levels correlate with the reproductive behaviors of these female orangutans using associated behavioral data. Our results suggest that matings are more common during the periovulatory period than outside of it, but do not support a consistent link between hormonal indices of fecundability and mating behaviors in these individuals.

Preliminary findings of age and male sexual characteristics andand potential effect to semen characteristics and cryopreservation of the critically endangered Bornean orangutan in Malaysia

Bornean orangutan is a critically endangered non-human primate; however, the threat of extinction is not merely from poaching and habitat loss. Orangutan survival is also threatened by the genetic loss and genetic bottleneck due to the low effective population, prompting the dire need for an immediate genetic preservation program through systematic biobanking and assisted reproductive technology (ART). This study aims to provide integral data to the semen characteristics, extension, and cryopreservation of the Bornean orangutan and the potential relationship to male traits. Five captive orangutans from Sepilok Orangutan Rehabilitation Centre (SORC) with a mean body weight of 52.81 ± 7.00kg were used for this study. Semen collection was performed using electroejaculation (EE) under complete general anesthesia. Semen was subjected to macroscopic and microscopic evaluation while testicular measurement was obtained using digital calipers. The semen characteristics of the orangutans are volume (778 ± 250.21µl), pH (7.80 ± 0.25), concentration (32.38 ± 17.40 × 106 sperm/ml), total motility (61.00 ± 12.88%), adjusted motility index (48.76 ± 11.32%), live spermatozoa (77.75 ± 6.94%) and normal spermatozoa (11.48 ± 11.34%). Analysis of variance statistical analysis test was used to compare the significant difference between means, at (p < 0.05). Spermatozoa concentration was the only significant different parameter between individuals. Testes biometry parameters are statistically significant between the flanged and unflanged individuals. Live spermatozoa are different in adult and sub-adult individual while teratospermia was found to be consistently high in all individuals. Chilled and post-thaw quality after cryopreservation suggests promising survivability of spermatozoa. Semen collection with EE yields a consistent and acceptable quality of spermatozoa for cryopreservation, biobanking purposes, and potential application of ART.

The Gospel According to Ah Meng: Conservation, Celebrity, and the Singapore Story

Abstract This essay reflects on the story of probably the world’s most famous captive orangutan, Ah Meng, who died in 2008 but has since been “replaced” by her granddaughter, Ishta, who took over as the “new face” of Singapore Zoo in 2016. Ah Meng’s story is interesting for what it conceals and what it reveals, including the recent history of wildlife trafficking in Southeast Asia, for which Singapore – despite its conservationist credentials – acts as an important hub. Ah Meng’s rescue and rehabilitation also raises interesting questions about the Singapore Story, the popular name given to the heroic narrative of historical survival and economic development associated with Singapore’s ruling party, the PAP (People’s Action Party), and especially its former leader, Lee Kuan Yew. This essay considers the extent to which Ah Meng is more than just a national ambassador animal or a global conservation icon, but is also a multifaceted figure for Singapore’s progress in an unevenly developed capitalist world order in which it insists on playing a leading part.

Orangutan (Pongo pygmaeus wurmbii) human-oriented behavior at the Lamandau Wildlife Reserve, Indonesia

Abstract. Amalia N, Fajarallah DP, Atmoko SSU. 2021. Orangutan (Pongo pygmaeus wurmbii) human-oriented behavior at the Lamandau Wildlife Reserve, Indonesia. Biodiversitas 23: 396-402. Unlike other conservation efforts, rehabilitation and reintroduction face different challenges due to humans' role in developing orphaned orangutans. Long-term interactions between orangutans and humans in captivity will gradually encourage orangutans to shape and increase human-oriented behavior (interest in humans). The Lamandau Wildlife Reserve (Lamandau WR) applies the soft-release method for the orangutan release process. Additional feed is provided every day. The correlation between animal-human interactions as part of the conservation efforts evaluation is essential to study. This study aimed to analyze the human-oriented behavior of the orangutan population in the Lamandau WR based on the observation sites (camp, feeding site, and forest) and the age-sex class of orangutans. This study also identified the factors that affected them. The study was conducted for five months (July-November 2019). During the observation, the factor of current age was suspected to affect human-oriented behavior. Human-oriented behavior is divided into three categories based on the orangutans' response: approaching, avoidance, and no response. The highest rates of human-oriented behavior in orangutans were approaching category. The highest promoting behavior was noticed at the camp and feeding site, especially young orangutans and mothers. Interest in humans can be explained by the 'captive effect' phenomenon where captive orangutans are more likely to independently explore the novelty (unfamiliar objects/foods) than their wild counterparts.

Carbon and nitrogen stable isotopic offsets between diet and hair/feces in captive orangutans.

Estimating stable isotopic offset values is crucial for dietary reconstructions. Although research into stable isotope ecology of wild nonhuman primates is increasing overall, only a minority of studies involve laboratory experiments. This study is the first to report the carbon and nitrogen stable isotopic offset values in hair and feces of orangutans. During an experiment lasting 1week, the weight of each consumed food item was recorded for each of six captive Bornean orangutan (Pongo pygmaeus)individuals. The food, hair, and fecal samples were collected for a few days, and their stable carbon and nitrogen isotope ratios were measured using an elemental analyzer/isotope ratiomass spectrometer. Subsamples of feces were treated with ethanol during the preservation process. Monte Carlo analyses showed that the 95% confidence intervals (CIs) of the carbon and nitrogen offset values between hair and diet were +0.9‰ to +3.9‰ and +2.3‰ to +4.5‰, respectively. The 95% CIs of the carbon and nitrogen offset values between feces and diet were -3.7‰ to -0.9‰ and +0.3‰ to +2.7‰, respectively. The effect of ethanol treatment on the stable isotope ratios of feces was unclear and inconclusive. The computed offset values of hair in captive orangutans are similar to those reported in other nonhuman primates, although those of feces showed greater interspecies variations. The offset values estimated in this study contribute to isotopic studies into the feeding ecology of free-ranging orangutans who are critically endangered in most wild settings.

USE OF COMPUTED TOMOGRAPHY (CT) TO DETERMINE THE SENSITIVITY OF CLINICAL SIGNS AS A DIAGNOSTIC TOOL FOR RESPIRATORY DISEASE IN BORNEAN ORANGUTANS (PONGO PYGMAEUS).

Orangutans are noteworthy among great apes in their predilection for chronic, insidious, and ultimately fatal respiratory disease. Termed Orangutan Respiratory Disease Syndrome (ORDS), this cystic fibrosis-like disease is characterized by comorbid conditions of sinusitis, mastoiditis, airsacculitis, bronchiectasis, and recurrent pneumonia. The aim of this retrospective study was to determine the sensitivity of clinical signs in the diagnosis of ORDS in Bornean orangutans (Pongo pygmaeus) compared with the gold standard for diagnosis via computed tomography (CT). We retrospectively compared observed clinical signs with CT imaging in a population of clinically affected animals at an orangutan rescue center in southeastern Borneo. From August 2017 to 2019, this center housed 21 ORDS-affected animals, all of which underwent CT imaging to delineate which areas of the respiratory tract were affected. We reviewed clinical signs recorded in medical records and keeper observation notes for each individual for the period of 2 years prior to the date of the CT scan. A chi-square test of association was used to assess whether the observed clinical signs could predict the results of CT imaging. Results show that clinical signs may not be sensitive indicators in predicting respiratory disease identified by CT imaging. Based on the results of this study, clinical signs appear to be very poor predictors of underlying respiratory pathology in orangutans, based on high P-values, low sensitivity, and low specificity. This result is observed even with clinical signs data gathered over a full 24-mo period prior to CT scan performance. The findings of this study suggest the need for advanced imaging to properly diagnose and manage the most common health issue of captive orangutans.

Activity Budget and Postural Behaviors in Orangutans on Bukit Merah Orang Utan Island for Assessing Captive Great Ape Welfare

ABSTRACT Assessments of the welfare status of captive and semi-captive animals often compare how their expression of natural behaviors differs from that of free-ranging conspecifics. From December 2015–2016, we recorded and analyzed the activity budget and postural behaviors of three orangutans in Bukit Merah Orang Utan Island (BMOUI) to evaluate their welfare status. The orangutans’ activity budget was dominated by resting (60%), feeding (13%), playing (14%), and moving (9%). Behavioral categories followed a similar trend: resting > feeding > moving > playing, except that the subadult male spent significantly more time playing than the two adults. The most predominant posture was sitting (47.0%), followed by pronograde standing (29.4%), lying (10.5%), and clinging (4.5%). Our results suggest that orangutans on BMOUI engage in less feeding but more resting, and show less postural diversity than free-ranging individuals. We propose that appropriate interventions to shift activity budgets, especially feeding vs. resting, and postural behaviors of captive orangutans toward those found in free-ranging orangutans might be beneficial for their welfare and survival.

The activity budgets of captive orangutan (Pongo pygmaeus) in two different Indonesian zoos

Abstract. Dalimunthe NP, Alikodra HS, Iskandar E, Atmoko SSU. 2021. The activity budgets of captive orangutan (Pongo pygmaeus) in two different Indonesian zoos. Biodiversitas 22: 1912-1919. In nature, orangutans spent most of the activity budgets for foraging. It is presumed that orangutan in captivity carries out different activities. This study aimed to investigate the activity of Bornean Orangutan (Pongo pygmaeus) in different zoos with different types of cage according to their design, enrichments, and diets. It was conducted at two different Indonesian zoos, namely Ragunan Zoo (TMR) and Taman Safari Indonesia (TSI). The study used a total of 11 orangutans. From each zoo, orangutan individuals from each zoo and each age and sex class (adult male, adult female, and juvenile/juvenile) were used in the present study. The activity budgets were compared between age-sexual classes, cage types, and weekday-holiday categories. Environmental enrichment analysis was also used. The total observation time in this study for each individual was 4,500 minutes. The adult male had more resting activity than other groups, while the juvenile group showed a higher percentage of social, moving, and feeding activities. It was also observed that there were different activity budgets between individuals in a separate cage and week-holiday groups. Overall, it was assumed that the difference in orangutan activity budgets was influenced by cage types, individual arrangements, and environmental enrichment. Meanwhile, a juvenile showed a higher percentage of enrichment using. Enrichment was influenced by cage type and individual arrangement.

Orangulas: effect of scheduled visual enrichment on behavioural and endocrine aspects of a captive orangutan (Pongo pygmaeus)

Captivity may have adverse effects on captive great apes, who would spend much more of their time engaged in foraging and other activities in the wild. Enrichment interventions have the potential to alleviate the adverse effects of captivity by introducing novel stimuli. In orangutans, interactive digital enrichment has proven effective at engaging users out of their own free will, in exchange for nothing but the experience. In this article, we report the results of scheduled visual enrichment in the form of “orangulas” - one-hour long videos of footage consisting mainly on open spaces in different environments, with which our pongid participant could engage at free will. We measured the efficacy of our orangulas with both behavioral and endocrine measurements, concluding that scheduled visual enrichment has the potential to improve the welfare of captive orangutans by providing novel stimuli in the context of largely stable environments.

Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans

Abstract. Abdul-Manan MN, Mohd-Ridwan AR, Aifat NR, Osman NA, Abdul-Latiff MA, Dharmalingam S, Md-Zain BM. 2020. Short Communication: Effectiveness of nuclear gene in species and subspecies determination of captive orangutans. Biodiversitas 21: 3665-3669. Genetic identification of captive orangutans is of paramount importance in providing a correct identity that is essential for captive management. Thus, the utility of nuclear DNA sequences was tested in this study to identify the genetic identity of captive orangutans at Bukit Merah Orang Utan Island. Out of 24 DNA samples that were successfully extracted, only 10 orangutan samples were successfully sequenced for the von Willebrand factor (vWF) gene. From the results, this gene was able to separate the genus Pongo at the species level. Distance and character analyses indicated that a clear separation between P. pygmaeus and P. abelii at the species level. However, the degree of separation at species level was indicated in tree topology with moderate bootstrap values. At the subspecies level of P. pygmaeus, this gene was unable to show a clear separation between three Bornean subspecies. All the subspecies were formed clade together with each other. The vMF gene is a good nuclear gene for the study of phylogenetic relationships of orangutans in captivity at the species level, but the genetic identification at subspecies level in the genus level remains unclear. We suggest that future studies should involve multiple independent nuclear markers to increase the probability of getting reliable results.

Low relationship quality predicts scratch contagion during tense situations in orangutans (Pongo pygmaeus).

Primates show various forms of behavioral contagion that are stronger between kin and friends. As a result, behavioral contagion is thought to promote group coordination, social cohesion, and possibly state matching. Aside from contagious yawning, little is known about the contagious effect of other behaviors. Scratching is commonly observed during arousal and as such may play a role within group dynamics. While the Bornean orangutan (Pongo pygmaeus) is commonly considered the least social great ape, orangutans do engage in social interactions. Therefore, their social organization makes them a suitable case for studying the social function of behavioral contagion. Through behavioral observations of captive orangutans, we recorded all yawn and scratch events together with the corresponding behavior of all bystander group‐members. As yawning was rarely observed, no conclusions could be drawn regarding this behavior. Scratching was contagious and occurred within 90 s after the triggering scratch. Specifically, orangutans showed increased scratch contagion when they had seen a weakly bonded individual scratch during tense contexts. When the orangutan had not seen the triggering scratch, the contagiousness of scratching was not affected by context or relationship quality. Our results indicate that behavioral contagion is not simply higher between individuals with stronger social relationships, but that the contagiousness of behaviors may vary based on the context and on social factors. We discuss these findings in light of an adaptive function that may reduce aggression.

Potentially lethal cystic fibrosis gene variant in the orangutan.

A syndrome of chronic upper and lower airway disease leading to increased morbidity and mortality occurs primarily in captive orangutans. Similarities in symptoms to the inherited human respiratory disease, cystic fibrosis, led us to hypothesize that orangutan respiratory disease is a result of variants in the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. We identified the nonsense variant, c.484A>T (p.Lys162X), in heterozygosity in an unaffected orangutan. Analysis of the pedigree of this orangutan confirmed that both his sire and deceased fetus also harbored the c.484A>T allele. An expression minigene harboring c.484A>T produced no full-length CFTR protein in HEK293 cells. Finally, the c.484A>T CFTR messenger RNA abundance was severely reduced in primary nasal epithelial cells of the orangutan indicating that c.484A>T (p.Lys162X) is potentially lethal. Genetic screening of the captive orangutan population could be used to prevent transmission of this potentially lethal variant, and thus aid in the conservation of this critically endangered species.

Narrative inquiry, creative nonfiction and two braided stories of the rehabilitation and release of orang-utans in Sebuyau, Sarawak

The rehabilitation and release of captive orang-utans remains a contentious issue in the conservation of the endangered species. In the 1970s, three confiscated and captive-raised orang-utans were released at Sebuyau, Sarawak: using narrative inquiry as the research method, the researcher–writer tells the stories of two people instrumental in this experiment. Their stories tell of personal experience working with the rescued animals and the consequences of well-meaning but ill-fated human actions. In this creative nonfiction work, the voices of the wildlife officer, forest guard and researcher–writer take the reader through a gamut of emotions: wonder, compassion, frustration and sorrow. This writing is part of the researcher–writer’s postgraduate work using creative nonfiction to tell the stories of men and women working to conserve the orang-utan over a span of fifty years in Sarawak, Malaysian Borneo.