Urinalysis is a rapid, non-invasive method used to obtain diagnostic information on primates. While several studies have investigated dipstick and specific gravity values in chimpanzees, urine sediment analysis is often excluded. Crystalluria, observed during urine sediment analysis, can be benign or indicate renal pathologies. In total, 665 urine samples from sanctuary-housed chimpanzees were analyzed over the course of 17 months for pH, specific gravity, time of collection, and crystalluria. Calcium salt crystalluria was seen in 9.0% of the samples from 23.7% of the individuals in the study. Urinary pH and specific gravity were significantly higher in samples with crystalluria than in those lacking crystalluria, while time of collection did not differ between the two groups. While diet is the most likely cause of the crystalluria in this population, several medications could also cause urinary crystallization. Further exploration of the significance of calcium salt crystalluria in chimpanzees is warranted.