Background: The farmers are rearing duck in small scale in semi intensive system without investing more capital but it contributes a major part to uplift the socio-economic condition of the rural poor people of Assam. The Pati duck (Anas platyrhynchos domesticus) population constitutes a major indigenous non-descript economically important duck variety in the state of Assam. The Harderian gland acts as a part of the head associated lymphoid tissue (HALT) and provides local innate immunity to the upper respiratory system, to the eye and oral cavity. Since literature on histological study of Harderian gland of Pati duck is scant, hence the present study was designed to establish the basic histological details on Harderian gland at different stages of postnatal development of Pati duck of Assam. Methods: The present study was conducted on total forty five (45) numbers Pati duck of Assam at different stages of postnatal development. The experimental birds were brought to the Department of Anatomy and Histology, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati-22 and was sacrificed according to the method of Gracy (1986). For histological study the tissue samples from the Harderian gland of all the age group were fixed in 10% neutral buffered formalin solution and processed to prepare paraffin blocks as per the procedure described by Luna (1968). The paraffin blocks were sectioned in Shandon Finesse microtome in 5 µm thickness and the sections were stained by different methods as described by Luna (1968). Result: The Harderian gland of Pati duck had a capsule, the thickness of the capsule of the Harderian gland was increased slightly along the advancement of the age. The capsular connective tissue penetrated the parenchyma of the Harderian gland in the form of septa. The collagen, reticular, elastic and nerve fibers were observed along with the capsule and as well as in the septa. The Harderian gland of the Pati duck was compound tubular gland. The columnar epithelial cells lined the tubules. There was no cortex and medulla in the gland and the tubular structure were uniformly distributed within the lobules. A central canal was present in the gland which converged to the duct of the gland. The lymphocytes, plasma cells were found in the intertubular connective tissue. The numbers or population of plasma cell and lymphocytes were accelerated by the advancement of age. The myoepithelial cells with spindle shaped nucleus were present near the basement membrane close to the secretary cells. The histology of the duct of the Harderian gland of Pati duck was like that of typical hollow tubular organs. There were few lymphatic nodules very distinctly present extended from the lamina propria to the muscle layer.