Capsicum Annuum Extract Research Articles

Investigation of novel, green synthesized and photocatalytic Sn-doped MoO3 nanomaterial for the degradation of methyl orange

Nowadays, water resources are being contaminated owe to the waste organic pollutants. A novel Sn-doped MoO3 nanomaterial was developed in the presence of extract from the skin of Capsicum annuum fruit to decontaminate the waste water by degrading this organic pollutant. For the preparation of these nanomaterials, a facile green synthesis approach was adopted by using Capsicum annuum extract. The as designed nanomaterials were thoroughly characterized by X-ray diffraction spectroscopy (XRD, scanning electron microscopy (SEM), ultraviolet visible spectroscopy (UV–vis) and photoluminescence spectroscopy (PL) techniques. The photocatalytic activity of as designed nanomaterials was observed against the methyl orange (MO) a model dye under the irradiation of visible light. The results obtained from the characterization of these nanomaterials have shown reduced sized particles with cone, sphere and rod like morphologies. In comparison to pure MoO3 prepared without extract (Sample-1) and MoO3 prepared with extract (Sample-2), the Sn-doped MoO3 prepared in the presence of extract (Sample-3) has shown a reduced optical energy bang gap value i.e 2.84 eV and an enhanced photocatalytic activity against MO i.e about 95%. This optimized sample has also shown a low charges recombination rates. This excellent photocatalytic performance is credited to the increased active sites owe to the development of large surface area and a significant decrease in the recombination rate of charge carriers along with a reasonable decrease in optical band gap. These results have exhibited a boosted photocatalytic performance of this material in visible light region.

Effect of dietary intervention with Capsicum annuum extract on growth performance, physiological status, innate immune response, and related gene expression in Nile tilapia

The red pepper (Capsicum annuum) has gained great attention recently because of its biological and pharmacological characteristics. The present approach aimed to evaluate the effects of C. annuum alcoholic extract (CAE) supplementation on Nile tilapia (Oreochromis niloticus) growth performance, physiological status, some metabolic, immune, and regulatory genes expression, and resistance against Streptococcus agalactiae infection. Fish (22.26 ± 0.19 g) were assigned to four treatments (five replicates, each with 10 fish replicate−1) and fed tested diets for 60 days. The experimental diets were supplemented with CAE at 0, 0.4, 0.8, and 1.6 g kg−1, expressed as CAE0, CAE0.4, CAE0.8, and CAE1.6, respectively. The findings exhibited that CAE dietary supplementation improved growth performance, feed utilization, elevated growth hormone level, and digestive enzyme activities (amylase and protease), and lowered leptin hormone in a level-dependent manner. Boosting the mRNA expression of the transporter proteins (solute carrier family 15 member 2 and solute carrier family 26 member 6) and insulin-like growth factor-1 genes with a decrease in the myostatin gene expression was noticed in the CAE-fed groups. The innate immune (serum bactericidal activity %, complement 3, and phagocytic activity %) and antioxidant (glutathione peroxidase and total antioxidant capacity) parameters were significantly (p < 0.05) improved, and the serum malondialdehyde level was significantly decreased by CAE dietary inclusion. A marked upregulation in the mRNA expression of interleukins (il-1β, il-6, il-8, and il-10), transforming growth factor-β, glutathione peroxidase, and glutathione synthetase genes were observed in CAE-fed groups. Dietary CAE decreased the cumulative mortalities after the challenge with S. agalactiae by 20, 13.33, and 10% in CAE0.4, CAE0.8, and CAE1.6, respectively, compared to the control (40%). Overall, dietary supplementation with CAE could improve growth performance and physiological status, and modulate the expression of several regulatory genes in Nile tilapia. The recommended level of CAE is 1.6 g kg−1 to augment growth and health status.

Modification and development of optical, thermal, dielectric properties and antibacterial activity of PVA/SA blend by Ag/Se nanofillers: Nanocomposites for energy storage devices and food packaging applications

Silver nanoparticles (Ag NPs) were synthesized using Capsicum annuum extract, which is a cost-effective and green technique. At the same time, selenium nanoparticles (Se NPs) were prepared by sol-gel technique. Then, a series of PVA/SA blend samples filled with different contents of Ag–Se nanoparticles were prepared via the solution casting way. The nanocomposite films were investigated using different measurements. XRD scans of the nanocomposites displayed a decrease in the crystallinity of the PVA/SA blend with increasing the content of Ag–Se NPs as nanofillers in the PVA/SA matrix. FT-IR spectra indicated the complexation and positive interactions between the PVA/SA blend and Ag–Se NPs. The thermal stability of the polymeric samples was improved after the addition of the nanofiller (Ag–Se NPs), as shown by TGA scans. Moreover, the indirect/direct optical energy gap decreased from 3.96/5.28 to 2.73/3.59 eV with increasing concentrations of Ag–Se NPs, confirming the improvement in the optical properties of the filled polymeric samples, as indicated by UV/visible spectra. Dielectric studies revealed that the filling of Ag–Se NPs increases the defects and leads to a greater number of dipoles within the blend and, thus, an increase in the dielectric constant and dielectric loss of the nanocomposite samples. The antibacterial test showed that all filled samples had broad antibacterial activity against Gram-positive and Gram-negative bacteria compared to pure PVA/SA blend and that it was linearly related to the nanofillers concentrations in the polymeric matrix. Improvements in thermal, optical, and electrical properties make the present nanocomposite samples suitable for multiple functions in many applications, such as high-density energy storage and flexible electronic devices. In contrast, the enhancement in the antibacterial activity could indicate that these nanocomposites can also be used for antibacterial packaging and biomedical purposes.

Inhibition of bacterial virulence factors of foodborne pathogens by paprika (Capsicum annuum L.) extracts

Capsicum annuum extracts of different polarities were tested for their antimicrobial, antibiofilm, and anti-Quorum sensing (QS) activity against several pathogenic microorganisms and possible toxic effects using an in vivo model (Galleria mellonella L.). Hexane (HE) and Chloroform extract (CE) were the most active in inhibiting biofilm formation of the eight bacteria tested, with inhibition percentages between 22 and 88% for a concentration of 100 μg/mL. These extracts were potent inhibitors of Staphylococcus aureus biofilm, and microscopy analysis confirmed a significant reduction in adhered cells. Moreover, polystyrene surfaces coated with these extracts decreased Staph. aureus adhesion resulting in biofilm formation and activity inhibition. In addition, they interfere with the hemolysin and coagulase activities. The extracts inhibited biofilm formation and swarming motility in Gram-negative bacteria. Furthermore, in Pseudomonas aeruginosa, elastase activity and pyocyanin production were reduced. In the most active extracts, the main compound was linoleic acid, followed by oleic and hexadecanoic acids. Paprika extracts capable of controlling bacterial virulence represent a promising alternative as a natural preservative to restrict contamination or spoilage of food.

Antibacterial and Therapeutic Potentials of the Capsicum annuum Extract against Infected Wound in a Rat Model with Its Mechanisms of Antibacterial Action.

The wound healing process is essential to reform the damaged tissue and prevent its invasion by pathogens. The present study aims at evaluating the antibacterial and therapeutic properties of the Capsicum annuum L. (Solanaceae) extract against infected wound in a rat model with its mechanisms of antibacterial action. The fruit extract was prepared by maceration in methanol. The broth microdilution method was used to investigate the antibacterial activity of the methanol extract of C. annuum fruits. The therapeutic effect of the extract gel was performed on an excision wound infected with Staphylococcus aureus using a rat model. The total phenol, flavonoid, and tannin contents as well as the antibacterial mechanisms of action of the extract were determined using spectrophotometric methods. The C. annuum fruit extract showed antibacterial properties which can be linked to its total phenolic, flavonoid, and tannin contents. The antibacterial activity is due to the inhibition of the biofilm formation, ATPases/H+ proton pump, and dehydrogenase activity as well as the alteration of the bacterial cell membrane through the leakage of nucleic acids, reducing sugars and proteins. The extract gel showed a significant (p < 0.05) increase in the percentage of wound closure and eradicated S. aureus at the infection site. The extract gel was nonirritating to the skin and slightly irritating to the eyes and should be used with caution. Overall, the findings of the present study support the traditional use of the studied plant in the treatment of wounds and infectious diseases associated with the tested bacteria.

Oral Capsaicinoid Administration Alters the Plasma Endocannabinoidome and Fecal Microbiota of Reproductive-Aged Women Living with Overweight and Obesity.

Capsaicinoids, the pungent principles of chili peppers and prototypical activators of the transient receptor potential of the vanilloid type-1 (TRPV1) channel, which is a member of the expanded endocannabinoid system known as the endocannabinoidome (eCBome), counteract food intake and obesity. In this exploratory study, we examined the blood and stools from a subset of the participants in a cohort of reproductive-aged women with overweight/obesity who underwent a 12-week caloric restriction of 500 kcal/day with the administration of capsaicinoids (two capsules containing 100 mg of a capsicum annuum extract (CAE) each for a daily dose of 4 mg of capsaicinoids) or a placebo. Samples were collected immediately before and after the intervention, and plasma eCBome mediator levels (from 23 participants in total, 13 placebo and 10 CAE) and fecal microbiota taxa (from 15 participants in total, 9 placebo and 6 CAE) were profiled using LC–MS/MS and 16S metagenomic sequencing, respectively. CAE prevented the reduced caloric-intake-induced decrease in beneficial eCBome mediators, i.e., the TRPV1, GPR119 and/or PPARα agonists, N-oleoyl-ethanolamine, N-linoleoyl-ethanolamine and 2-oleoyl-glycerol, as well as the anti-inflammatory N-acyl-ethanolamines N-docosapentaenyl-ethanolamine and N-docosahexaenoyl-ethanolamine. CAE produced few but important alterations in the fecal microbiota, such as an increased relative abundance of the genus Flavonifractor, which is known to be inversely associated with obesity. Correlations between eCBome mediators and other potentially beneficial taxa were also observed, thus reinforcing the hypothesis of the existence of a link between the eCBome and the gut microbiome in obesity.

Sol-gel synthesis of DyFeO3/CuO nanocomposite using Capsicum Annuum extract: Fabrication, structural analysis, and assessing the impacts of g-C3N4 on electrochemical hydrogen storage behavior

Nano-sized DyFeO3/CuO composites were fabricated via a green pechini sol-gel route using Capsicum annuum L. extract as a natural chelating agent. Capsicum annuum L. chelating agent contains a set of environmentally friendly biomolecules, which control the nucleus and the growth of formed crystals by creating a space barrier around the cations, and ultimately preventing the accumulation of nano-products. Changing of parameters in synthesis reaction consisting of chelating agents (natural and chemical) and calcination temperature, for its part, offer a righteous grip on the nano-composites dimension and morphology which pure DyFeO3/CuO nano-composites attained. The as-prepared DyFeO3/CuO nanostructures were investigated through Fourier transform infrared (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), BET, vibrating sample magnetometer (VSM), and energy-dispersive X-ray (EDX) analysis in terms of structure, uniformity, porosity, shape, and size. The effect of adding CuO and graphite carbon nitride (g-C3N4) nanostructures was evaluated on discharge capacity and cyclic voltammetry methods. The attained DyFeO3/CuO/g-C3N4 nano-products could be utilized as a promising new nanocomposite for electrochemical hydrogen storage. It was found that DyFeO3/CuO/g-C3N4 nanocomposites have a special hydrogen storage accomplishment due to the synergistic effects of CuO and g-C3N4 components, which can increase the storage capability to 1600 mAhg−1 after 20 cycles by facilitating the hydrogen charge-discharge process.

The Beneficial Effects of Red Sun-Dried Capsicum annuum L. Cv Senise Extract with Antioxidant Properties in Experimental Obesity are Associated with Modulation of the Intestinal Microbiota.

Capsicum annuum L. cv Senise is a sweet pepper containing health promoting compounds that can be modified by ripening and drying. This study focuses on finding the peppers with the best antioxidant properties, which are evaluated on an experimental model of obesity. Phytochemical profile and antioxidant activity are evaluated on several peppers obtained from the same cultivar at different ripening stages. Red sweet peppers show the highest content in polyphenols, β-carotene, lycopene, and capsinoids, and demonstrate the best antioxidant activity in vitro. Mice fed a high fat diet are orally treated with an extract from these peppers (Capsicum annuum extract [CAE]) (1, 10, and 25mg/kg/day). It promotes weight loss and improves plasma markers related to glucose and lipid metabolisms. CAE also ameliorates obesity-associated systemic inflammation reducing the expression of pro-inflammatory cytokines in adipose and hepatic tissues and improving the expression of different markers involved in the gut epithelial barrier function. These effects are associated with a modulation of the intestinal microbiome, which appears altered. The extract can be considered a new potential approach for the treatment of obesity, complementary to dietary restrictions.

Assessment of the insecticidal property of the pepper Capsicum annuum extracts on the larval stages of the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae).

The aqueous fruit extracts of the pepper Capsicum annuum L. (Solanales: Solanceae) were tested against the four larval stages of the tomato leafminer (TLM) Tuta absoluta Povolny (Lepidoptera: Gelechiidae). Random samplings were made from two greenhouses of Biskra, in the Southeast of Algeria. About 750 to 800 plants of the Sahara variety of tomatoes were planted in both greenhouses. In the laboratory, conditions with temperature, relative humidity (RH) and the photoperiod were 25° to 30°C, from 50% to 60% and 16L: 8D, respectively. A breeding of our species in the laboratory was made during six months of the study. For the toxicological assessments, three concentrations were chosen; 50 mg/L, 100 mg/L and 200 mg/L of the extracts. The results revealed that the extracts of C. annuum were very effective against the larvae of T. absoluta. After 24 h post-treatment, the mortality was less than 25% compared to that after 48h and 72h post-treatment which exceeded 50%. There was a non-significant difference in mortality rates between the four larval stages (F3, 140 = 1.12; P = 0.344). In contrast, highly significant differences existed between the time of exposure (F2, 141 = 26.2; P = 0.001) and the concentrations under study (F3, 140 = 59.6; P = 0.001).&#x0D; J. bio-sci. 26: 25-30, 2018

The Inhibitory Effect of Essential Oils on Rhizopus stolonifer, Trichophyton mentagrophytes, and Microsporum gypseum.

Rhizopus stolonifer is an opportunistic fungus that causes respiratory infections, sinusitis, and otomycosis. Trichophyton mentagrophytes and Microsporum gypseum cause athlete's foot, ringworm, and nail infections. Previous research has shown that some essential oils inhibit fungal growth. We investigated the effects of 0.5% and 1% concentrations of Rosmarinus officinalis, Cinnamomum verum, Citrus paradisi, and Capsicum annuum extracts on the growth of R. stolonifer and T. mentagrophytes. The combined effects also were determined at 0.5% concentration for these fungal species and for M. gypseum. Fungal species were grown in flasks, along with growth media, for 7, 14, and 21 days, with each species of essential oil or combination of oils and mass determined and compared to its control. Rosmarinus officinalis, Cinnamomum verum, and Citrus paradisi had overall significant inhibitory effects (P ≤.05) on R. stolonifer and T. mentagrophytes. C. annuum significantly (P ≤.05) inhibited the growth of T. mentagrophytes and enhanced that of R. stolonifer. The combination of essential oils resulted in inhibition of growth (P ≤.05) at similar percentages as R. officinalis, C. verum, and C. paradisi only. Essential oils such as Rosmarinus officinalis, Cinnamomum verum, and Citrus paradisi may be useful for treating opportunistic and dermatophytic fungal diseases.

Zero valent silver nanoparticles capped with capsaicinoids containing Capsicum annuum extract, exert potent anti-biofilm effect on food borne pathogen Staphylococcus aureus and curtail planktonic growth on a zebrafish infection model

Food plants Hungarian wax pepper (HWP) and Green Bell pepper (GBP), belonging to Capsicum annuum were utilized for biogenic fabrication of zero valent, nano-silver (AgNPs) through a photo-mediation procedure. In the bacterial strains evaluated, HWP/GBP AgNPs demonstrated effective bacteriostatic and bactericidal effect against Staphylococcus aureus. Time kill results portrayed that HWP/GBP nano-silver exhibited comparable bactericidal potency on S. aureus. Anti-biofilm potential of HWP/GBP AgNPs displayed significant effects at sub MIC levels, by triggering 50% biofilm reduction of the food spoilage microbe S. aureus, inferring that the anti-biofilm outcome is not dependent on antibacterial result, and this was confirmed by SEM and fluorescence studies. Histopathological analyses of S. aureus infected zebrafish liver did not display any abnormality changes such as extensive cell death and degeneration, upon treatment with HWP/GBP AgNPs and the zero-valent silver nanoparticles were comparatively less toxic and more operative in restraining the bioburden in S. aureus infected zebrafish model by a >1.7 log fold. Ability of light reduced HWP/GBP AgNPs to alleviate the in vitro and in vivo planktonic mode of growth and curb the biofilm formation of S. aureus is also demonstrated.

A study on the effect of temperature,PH and Capsicum annuum extract on the viability of Listeria monocytogenes in kariesh cheese

A study on the effect of temperature,PH and Capsicum annuum extract on the viability of Listeria monocytogenes in kariesh cheese

Reverse phase chromatographic behaviour of major components in Capsicum Annuumextract

BackgroundThe purpose of the study is to develop a suitable analytical method in order to establish appropriate conditions for isolation and assay of the dominant compounds in extracts of Capsicum annuum.ResultsThe studies are performed with standard substances to establish the HPLC conditions for complete separation of capsaicin and dihydrocapsaicin, the two major components of interest. Because of their prevalent apolar features, reverse phase chromatographic version was approached. Systematic studies on different eluents revealed the 65% methanol–35% water mixture as a proper mobile phase providing a complete separation of the components according to analytical claims.ConclusionsThe results may be of interest to develop a specific analytical procedure with advanced specificity for quantitative assay of capsaicin and dihydrocapsaicin in pharmaceutical products and in foods.

The Antioxidant Activity and Polyphenolic Contents of Different Plant Seeds Extracts

Different plant seeds extracts of Citrus sinensis, Hordeum sativum, Triticum sativum, Canna indica, Citrullus vulgaris and Capsicum annuum were evaluated for their antioxidant activity by the following methods: 2,2-diphenyl-1-pycril-hydrazyl (DPPH) radical scavenging, reducing power, RBCs hemolysis and linoleic acid oxidation, a long with the determination of total phenolic and flavonoids contents. All the methanolic extracts showed high antioxidant activity and have high contents of phenolic and flavonoid. The Canna indica extract exhibited strong antioxidant as a reducing power and as DPPH radical-scavenging (3.61 absorbance, 87.12%, respectively), while the Hordeum sativum extract exhibited highest inhibitory effect on RBCs hemolysis (59.55%) and the Capsicum annuum extract has highest inhibitory effect on linoleic acid peroxidation (65.06%).

Final Report on the Safety Assessment of Capsicum Annuum Extract, Capsicum Annuum Fruit Extract, Capsicum Annuum Resin, Capsicum Annuum Fruit Powder, Capsicum Frutescens Fruit, Capsicum Frutescens Fruit Extract, Capsicum Frutescens Resin, and Capsaicin1

Final Report on the Safety Assessment of Capsicum Annuum Extract, Capsicum Annuum Fruit Extract, Capsicum Annuum Resin, Capsicum Annuum Fruit Powder, Capsicum Frutescens Fruit, Capsicum Frutescens Fruit Extract, Capsicum Frutescens Resin, and Capsaicin1

Identification of the major vanilloid component in Capsicum extract by HPLC-EC and HPLC-MS.

A sensitive multi-channel HPLC-electrochemical (EC) method has been developed to determine the vanilloid content in the complex Capsicum annuum extract Capsibiol. Chromatographic separation was achieved within 10 min using a YMC Basic S5 column with a mobile phase containing chloroacetic acid, heptane sulphonic acid and acetonitrile. The multi-channel detector simultaneously applied four potentials between +500 and +800 mV (referenced to a silver/silver chloride electrode) to four glassy carbon working electrodes. The most abundant (0.94 mg/g) vanilloid analogue in the Capsibiol sample demonstrated an electrochemical reactivity and retention time similar to that of vanillic acid in HPLC-EC analysis. Its identity was confirmed by HPLC-MS using a Zorbax SB-CN column with a mobile phase containing formic acid and methanol.

Ingestion of chilli pepper (Capsicum annuum) reduces salicylate bioavailability after oral aspirin administration in the rat

The bioavailabilities of aspirin (acetylsalicylic acid) and of salicylic acid were studied in male Wistar rats after acute and chronic administration of a Capsicum annuum extract, containing 100 mg of capsaicin per gram. With a single administration of 100 mg/kg of the extract, aspirin blood levels remained unchanged, but salicylic acid bioavailability was reduced in 44% compared with control animals. With a single administration of 300 mg/kg of the extract, aspirin blood levels were undetectable while salicylic acid bioavailability was reduced in 59%. Chronic administration once daily for 4 weeks of 100 and 300 mg/kg of the extract resulted in undetectable aspirin blood levels, while salicylic acid bioavailability was reduced in 63 and 76%, respectively, compared with controls. Results show that Capsicum ingestion reduces oral drug bioavailability, likely as a result of the gastrointestinal effects of capsaicin.

Ingestion of chilli pepper (&lt;i&gt;Capsicum annuum&lt;/i&gt;) reduces salicylate bioavailability after oral aspirin administration in the rat

The bioavailabilities of aspirin (acetylsalicylic acid) and of salicylic acid were studied in male Wistar rats after acute and chronic administration of a Capsicum annuum extract, containing 100 mg of capsaicin per gram. With a single administration of 100 mg/kg of the extract, aspirin blood levels remained unchanged, but salicylic acid bioavailability was reduced in 44% compared with control animals. With a single administration of 300 mg/kg of the extract, aspirin blood levels were undetectable while salicylic acid bioavailability was reduced in 59%. Chronic administration once daily for 4 weeks of 100 and 300 mg/kg of the extract resulted in undetectable aspirin blood levels, while salicylic acid bioavailability was reduced in 63 and 76%, respectively, compared with controls. Results show that Capsicum ingestion reduces oral drug bioavailability, likely as a result of the gastrointestinal effects of capsaicin.