Capitella Sp Research Articles

Response of benthic assemblages to heavy metal contamination from shipbreaking operations in a tropical coastal area

The response of macrobenthic communities to both environmental and anthropogenic pressures serves as a valuable indicator of ecosystem integrity. This study aimed to unravel the benthic community structure, examine the heavy metal contamination status, and explore the response of the microbenthic community to heavy metals in a tropical shipbreaking region. For this, sediment and benthos samples were taken from different sampling stations of the shipbreaking area and the non-shipbreaking sites. The results showed the benthic community consisted of 23 taxa, including Polychaeta, Oligochaeta, Amphipoda, Decapoda, Gastropoda, Bivalvia, and Priapulida, with polychaetes being the predominant group. The benthic community and diversity values were significantly varied between the two sampling sites (p <0.05). Taxa richness was lower in the shipbreaking area, primarily characterized by the dominance of opportunistic benthic groups, particularly Capitella sp. The concentrations of heavy metals (µg. g−1) in the sediment varied within the following ranges: 35.54–258.021 for Zn, 4.84–132.08 for Pb, 22.82–75.25 for Cu, 198.74–764.16 for Mn, 14.57–42.13 for Cr, 4.03–42.23 for Ni, and 3.82–14.85 for Co. Based on concentration levels, Zn, Pb, and Cu were identified as major contaminants with moderate to high potential for adverse effects, particularly evident in the shipbreaking area compared to non-shipbreaking areas. Pollution indices, including enrichment factors and contamination factors, also highlighted contamination by Zn, Pb, and Cu. Among the benthic taxa, Polychaeta demonstrated a high degree of tolerance to elevated concentrations of these heavy metals. Amphipoda density was significantly negatively correlated with Zn, Pb, and Cu concentrations, with significant coefficients of determination (r2= 0.38, p <0.10; r2= 0.64, p <0.01 and r2 = 0.63, p <0.01, respectively). Multivariate analyses (cluster analyses, MDS and CCA) demonstrated both positive and negative relationships among heavy metals and benthic groups. Canonical Correspondence Analysis (CCA) showed the benthic community was negatively correlated with Zn, Pb, and Cu. This finding provided critical insights into the ecological health of aquatic ecosystems, aiding in the development of informed conservation and management strategies.

Diversity of Annelida Worm as a Bioindicator of Water Quality in Unus Estuary, Mataram Lombok

Condition of river is strongly influenced by the characteristics and the surrounding environment. Unus River is one of the rivers in Mataram City that flows throughout the year. The flow of the Unus River is used by residents for irrigation purposes, household waste disposal, market waste, industrial waste and so on. The purpose of this study was to determine annelida diversity index and to determine chemical and physical conditions of waters. The study was conducted by dividing into 10 stations by stratified random sampling. Physical and chemical parameters measured were pH, temperature, DO, BOD, C Organic, depth and turbidity. Data analysis is descriptively. The results showed that 6 species found in the estuary of Unus such as Tubifex sp, Branchiura sp, Nereis sp, Lumbriculus sp, Capitella sp and Halobdella sp. Ecosystem conditions are not balanced and there is a tendency to high ecological pressure. Physical and chemistry waters quality are still below the quality standard but there is a tendency DO deficit at each station.

The effects of teflubenzuron on mortality, physiology and accumulation in Capitella sp.

The chitin synthesis inhibitor teflubenzuron (TFB) is a feed antiparasitic agents used to impede molting of the salmon lice, an ecto-parasite that severely affects the salmon industry. Low absorption of oral administered TFB may cause elevated concentrations in the feces discharged from the salmon into the benthic environment. The polychaete Capitella sp. are often dominant in such habitats and consume organic waste deposited on the sediment. In the present study, Capitella sp. were exposed to doses of TFB in salmon feed of 1, 2 and 4 g TFB kg−1 (0 g TFB kg−1 in control group) over an experimental period of 32 days. Cumulative mortality was 12%–15% in both treatment groups with 1 and 2 g TFB kg−1 and reached 27% in the group with 4 g TFB kg−1. Only the highest dose (4 g TFB kg−1) negatively affected feed intake, growth and respiration of the polychaetes while food conversion efficiency was not affected. At the end of the experiment, the concentrations of TFB in the Capitella sp. were high, in the range of 9.24–10.32 μg g−1 for the three treatment groups. It was suggested that a maximum level of absorption rate was reached, also for the lowest dose. High concentrations of TFB in the Capitella sp. might pose a risk to crustaceans that forage for polychaetes in the vicinity of fish farms. We conclude that the effects of TFB on Capitella sp. may therefore primarily be to the predators rather than the Capitella sp.

Bioecology of macrobenthic communities in the microtidal monsoonal Kodungallur–Azhikode Estuary, southwest coast of India

AbstractA comprehensive study of the macrobenthic communities in the Kodungallur–Azhikode Estuary (KAE) was conducted during 2009–2011 period. A total of 18,846 organisms were collected, with 60% being malacostracans, followed by polychaetes (20%), molluscs (9%) and ‘others’ group (11%). A total of 79 species in 71 genera belonging to 49 families were identified, with 33 spp. being polychaetes, 26 spp. being malacostracans, 11 spp. being molluscs and 9 spp. being in the ‘others’ group. A single species of opportunistic amphipod (Americorophium triaeonyx) comprised more than 62.05% of the total numerical abundance of macrobenthos. The other dominant species were Obelia bidentata, Arcuatula senhousia, Cirolana fluviatilis, Prionospio cirrifera and Capitella sp. Canonical correspondence analysis (CCA) results indicated environmental parameters such as water column salinity, turbidity, sediment Eh, substratum type, chlorophyll‐a concentration, depth and organic matter in sediment were the significant factors influencing the distribution of macrobenthic species in the KAE. The present study provides baseline information for future KAE benthic studies.

TOXICIDAD AGUDA DEL MALATION 500® Y TYSON 4E® EN Capitella sp.

The toxicity of Malation 500 ® and Tyson 4E ® , whose active ingredients are malathion and chlorpyrifos ethyl, respectively, was experimentally determined using as test organism Capitella sp. collected in the Chetumal Bay and acclimated on lab. To do that, 48 h static toxicity bioassays were done using five nominal concentrations distributed in a logarithmic scale, a blank and three replicates for each bioassay chamber. Ten organisms were exposed to each concentration in crystallizer dishes with a sediment layer, saline solution and their respective volume of pesticide. Mortality was registered and median lethal concentrations (LC 50 ) were determined through the Probit analysis with 95 % confident intervals. The LC 50 were 27.79 ± 2.67 mg/L for Malation 500 ® and 7.46 ± 1.59 mg/L for Tyson 4E ® , therefore this last one is more toxic. ANDEVA showed significant differences in the tested concentrations (p < 0.05). Malation 500 ® was classified as slightly toxic and Tyson 4E ® as moderately toxic for Capitella sp. Both risk quotients of the pesticides (RQ < 1) indicate that the ecological risk is negligible.

Macrobenthic functional feeding groups in a microtidal monsoonal estuary (Kodungallur–Azhikode estuary, India)

The functional feeding groups of macrobenthos in the Kodungallur–Azhikode estuary were studied on a monthly basis for a two year period (2009–2011). The main aim of the study was to understand the distribution pattern of macrobenthic functional feeding groups and their relationship to environmental drivers in the estuary. Malacostracan crustaceans dominated the system (60%) by numerical density, while molluscs contributed significantly to macrobenthic biomass (63%). Many species exploit the same class of resources within an assemblage of macrofaunal groups. The suspension feeding corophiid amphipod Americorophium triaeonyx was the most represented macrofaunal species. Other dominant macrofaunal feeding groups were the suspension feeding hydroid colonies of Obelia bidentata, surface deposit feeding polychaetes Prionospio cirrifera, suspension feeding mussels Arcuatula senhousia, carnivorous isopods Cirolana fluviatilis and sub-surface deposit feeding polychaetes Capitella sp. The sampling stations were compared using taxonomic distinctness (AvTD), and variations in taxonomic distinctness (VarTD), the values of AvTD and VarTD for all stations during the entire monthly survey fell within the confidence intervals of probability funnel (95%, p<0.05). However, a significant variation was noticed in the values between stations (AvTD: F (6,161) = 3.105, p=0.007; VarTD: F (6,161) = 2.489, p=0.025). The abundance biomass curves were plotted separately for each site as well as combined for the entire estuary. The relatively high negative trend was observed in the station 5 (W=−0.139) which indicates potential for the relatively high disturbance among all other stations. However, the W-value for the entire estuary was -0.079, which depicted moderate disturbance.

Laboratory development of &lt;em&gt;Capitella&lt;/em&gt; sp. A (Annelida: Capitellidae) from a NW Mediterranean fish farm reared under different organic enrichment conditions

The polychaete Capitella sp. A, collected in a NW Mediterranean fish farm (Les Cases d’Alcanar, Tarragona, Spain), was cultured for the first time under experimental conditions with different organically enriched sediments to study the differences in development and growth. The species proved to be dioecious and had lecithotrophic development. Sizes of individuals and duration of the developmental stages varied widely, as in most known species of Capitella. In organically enriched sediments, the juveniles were seen one day after hatching and immature females (i.e. with yellow ovaries) after 52 days. Females may reach maturity (i.e. show white intra-coelomic oocytes) at about 64 days old, and the species had a life span of 167 days. According to its development, Capitella sp. A differs from all known lecithotrophic species of the genus. The results also proved that organically enrichment enhanced growth and survival, whereas lowering food can cause morphological alterations such as reduced size in male genital spines.

Acute toxicity and risk assessment of three commercial detergents using the polychaete Capitella sp. C from Chetumal Bay, Quintana Roo, Mexico

The acute toxicity of three formulations of commercial detergent (ROMA®, FOCA® and BLANCA NIEVES®) was evaluated using the polychaete Capitella sp. C in static bioassays over a 48-h exposure period. The probit method was used to determine the median lethal concentration (LC50) of each formulation as a whole as well as the LC50 of the active ingredient, linear alkylbenzene sulfonate (LAS), using 95 % confidence intervals. The formulations and LAS showed LC50 values of between 70.79 and 147.91 ppm and 15.48 and 22.38 ppm, respectively, at 48 h. FOCA® was the most toxic detergent, followed by BLANCA NIEVES® and finally ROMA®. The variation in the toxicity of the three detergents could have been caused both by differences in the relative concentrations of the anionic surfactant LAS contained in each formulation and the presence of other ingredients (enzymes, sodium silicate, sodium tripolyphosphate, bleachers and perfumes) which can also increase formulation toxicity. Correlation analysis revealed that percent mortality of Capitella sp. C increased with increase in the concentrations of the detergent over the 48-h exposure period. The risk quotient was greater than one for all three evaluated detergents, indicating that there is a high risk that they adversely affect the aquatic biota, particularly sediment-dwelling organisms such as the test species.

Benthic community response to sediment organic enrichment by Mediterranean fish farms: Case studies

There is a great deal of scientific information about the biogeochemical processes in sediments near fish farms and how those processes may be driven under nutrient enrichment to environmental negative effects, as biodiversity decrease is observed in benthic assemblages. Within an adaptive management framework, a good monitoring program can be used to adjust farm management to avert serious and persistent impacts to the sediment. Macrobenthic community represents a good indicator able to detect environmental effects resulting from organic enrichment and, based on this, was selected to monitor Italian marine fish farms. In order to assess the environmental sediment disturbance resulting from marine aquaculture along the Italian coasts three marine fish farms were considered based on their different environmental features: two located in the Tyrrhenian Sea and one in the Adriatic Sea. The selected cage fish farms produce European sea bass Dicentrarchus labrax (L.), gilthead sea-bream Sparus aurata (L.) and meagre Argyrosomus regius (A.) The study ranges from 2001 to 2012, at different times in each fish farm, and was planned according to the fattening cycles. The samples for benthic community analyses were collected by means of a van Veen grab in selected stations located from 0 to 1000m from the sea cages, the farthest as control stations. In total 134 stations were sampled for more of 20,000 individuals belonging to more than 450 species. Data analyses were performed with statistical methods and biotic indices were calculated. The results showed changes in benthic communities at the stations located just beneath the cages and connected with high cultured biomass. The impacted sites showed loss of biodiversity and increase of opportunistic species such as Capitella Sp. I and Nebalia strausi. Changes in the benthic communities were more or less evident in relation to substrata composition, hydrodynamic conditions and cultured biomass. The observed impact was limited to a few tens of meters around the sea cages of all the three studied fish farms.

Accumulation and effects of sediment-associated silver nanoparticles to sediment-dwelling invertebrates

Sediment is increasingly recognized as the major sink for contaminants including nanoparticles (NPs). Thus, sediment-living organisms are especially susceptible to NP exposure. Studies of the fate and effects of NPs in the sediment matrix are still in their infancy, and data from such studies are in high demand. Here, we examine the effects of exposure to sediment mixed with either aqueous Ag (administered as AgNO3) or Ag NPs (13nm, citrate-capped) at a nominal exposure concentration of 100μg Ag/g dry weight sediment on four benthic invertebrates: two clones of the gastropod Potamopyrgus antipodarum (clones A and B) and two polychaete species (Capitella teleta, Capitella sp. S). Our results show that both species sensitivity and Ag form (aqueous Ag, Ag NPs) play a role in bioaccumulation and effects. Following two weeks of exposure, both clones of P. antipodarum were found to be insensitive towards both Ag forms (generally low Ag accumulation, no toxicity). In contrast, the two Capitella species varied widely with respect to Ag uptake and observed toxicity. Capitella sp. S was adversely affected by both aqueous Ag (mortality) and Ag NPs (growth), whereas C. teleta was not affected by either Ag form. For neither polychaete species was the observed toxicity directly related to bioaccumulation. Therefore, future nano-ecotoxicological research should focus on understanding differences in uptake and handling mechanisms among species and the relationship between bioaccumulation and toxicity.

Nervous system development in lecithotrophic larval and juvenile stages of the annelid Capitella teleta.

BackgroundReconstructing the evolutionary history of nervous systems requires an understanding of their architecture and development across diverse taxa. The spiralians encompass diverse body plans and organ systems, and within the spiralians, annelids exhibit a variety of morphologies, life histories, feeding modes and associated nervous systems, making them an ideal group for studying evolution of nervous systems.ResultsWe describe nervous system development in the annelid Capitella teleta (Blake JA, Grassle JP, Eckelbarger KJ. Capitella teleta, a new species designation for the opportunistic and experimental Capitella sp. I, with a review of the literature for confirmed records. Zoosymposia. 2009;2:25–53) using whole-mount in situ hybridization for a synaptotagmin 1 homolog, nuclear stains, and cross-reactive antibodies against acetylated α-tubulin, 5-HT and FMRFamide. Capitella teleta is member of the Sedentaria (Struck TH, Paul C, Hill N, Hartmann S, Hosel C, Kube M, et al. Phylogenomic analyses unravel annelid evolution. Nature. 2011;471:95–8) and has an indirectly-developing, lecithotrophic larva. The nervous system of C. teleta shares many features with other annelids, including a brain and a ladder-like ventral nerve cord with five connectives, reiterated commissures, and pairs of peripheral nerves. Development of the nervous system begins with the first neurons differentiating in the brain, and follows a temporal order from central to peripheral and from anterior to posterior. Similar to other annelids, neurons with serotonin-like-immunoreactivity (5HT-LIR) and FMRFamide-like-immunoreactivity (FMRF-LIR) are found throughout the brain and ventral nerve cord. A small number of larval-specific neurons and neurites are present, but are visible only after the central nervous system begins to form. These larval neurons are not visible after metamorphosis while the rest of the nervous system is largely unchanged in juveniles.ConclusionsMost of the nervous system that forms during larvogenesis in C. teleta persists into the juvenile stage. The first neurons differentiate in the brain, which contrasts with the early formation of peripheral, larval-specific neurons found in some spiralian taxa with planktotrophic larvae. Our study provides a clear indication that certain shared features among annelids - e.g., five connectives in the ventral nerve cord - are only visible during larval stages in particular species, emphasizing the need to include developmental data in ancestral character state reconstructions. The data provided in this paper will serve as an important comparative reference for understanding evolution of nervous systems, and as a framework for future molecular studies of development.Electronic supplementary materialThe online version of this article (doi:10.1186/s12983-015-0108-y) contains supplementary material, which is available to authorized users.

Burrowing by small polychaetes - mechanics, behavior and muscle structure of Capitella sp.

Worms of different sizes extend burrows through muddy sediments by fracture, applying dorso-ventral forces that are amplified at the crack tip. Smaller worms displace sediments less than larger worms and therefore are limited in how much force they can apply to burrow walls. We hypothesized that small worms would exhibit a transition in burrowing mechanics, specifically a lower limit in body size for the ability to burrow by fracture, corresponding with an ontogenetic transition in muscle morphology. Kinematics of burrowing in a mud analog, external morphology and muscle arrangement were examined in juveniles and adults of the small polychaete Capitella sp. We found that it moves by peristalsis, and no obvious differences were observed among worms of different sizes; even very small juveniles were able to burrow through a clear mud analog by fracture. Interestingly, we found that in addition to longitudinal and circular muscles needed for peristaltic movements, left- and right-handed helical muscles wrap around the thorax of worms of all sizes. We suggest that in small worms helical muscles may function to supplement forces generated by longitudinal muscles and to maintain hydrostatic pressure, enabling higher forces to be exerted on the crack wall. Further research is needed, however, to determine whether surficial sediments inhabited by small worms fail by fracture or plastically deform under forces of the magnitudes applied by Capitella sp.

Effects of the antidepressant fluoxetine in spiked-sediments on developmental and reproductive features of the polychaetes Capitella teleta and Capitella sp A.

The cosmopolitan species-complex Capitella, a deposit-feeding polychaete, is widely used as an indicator of organic pollution and plays an important role in sewage waste cycling in marine and estuarine ecosystems. The antidepressant fluoxetine can be accumulated in sewage effluents and it could pose a hazard to infauna inhabiting surrounding areas. The study aimed to assess effects of fluoxetine on juveniles and adults of Capitella teleta and Capitella sp A. Sediments were spiked with four fluoxetine concentrations (0.001, 0.03, 0.3 and 3.3µg/g dry wt. sediment). Chronically exposed worms of C. teleta showed delay of maturity, delay or inhibition of copulation and morphological abnormalities (genital spines in males and juveniles) with adverse physiological consequences. Alternatively, in C. teleta fluoxetine enhanced proteroginous individuals that may be beneficial for the population. Worms of Capitella sp A only showed delay or inhibition of copulation. Observed fluoxetine adverse effects could have important ecological implications in natural populations of aquatic invertebrates due to the possible alteration or even inhibition of reproductive processes.

Morphological features and viability of Scrippsiella trochoidea cysts isolated from fecal pellets of the polychaete Capitella sp.

To investigate the impact of grazing by the polychaete Capitella sp. on the two cyst morphotypes of Scrippsiella trochoidea, the typical morphotype with short calcareous spines (spiny-type cyst) and artificially induced the transparent type without calcareous spines (naked-type cyst), we examined the morphological features and germination capability of the two cyst morphotypes isolated from fecal pellets of the polychaete Capitella sp. produced in a restricted habitat. The morphological destruction was observed in both spiny- and naked type cysts after passage through the gut of Capitella sp., and this seemed to occur rapidly for naked-type cysts. In addition, the germination of both spiny- and naked-type cysts isolated from fecal pellets on day 2 of harvesting was significantly reduced and subsequently completely abolished, in contrast to previous findings from ingestion studies. Our results indicate that continual grazing by Capitella sp. within a restricted habitat can compromise the survival of S. trochoidea cysts.

Interactive effects of hypoxia and PBDE on larval settlement of a marine benthic polychaete

Marine benthic polychaete Capitella sp. I is widely known to adapt to polluted habitats; however, its response to xenobiotics under hypoxic conditions has been rarely studied. This research aimed to test the hypothesis that interactive effects of hypoxia and congener BDE-47 of polybrominated diphenyl ethers (PBDE), which is ubiquitous in marine sediments, may alter the settlement of Capitella sp. I. Our results revealed that under hypoxic condition, settlement success and growth in body length of Capitella larvae were significantly reduced compared to those under normoxia of similar BDE-47 concentration. While no significant changes in morphology of settled larvae were noted in both exposure conditions, the presence of BDE-47 could enhance polychaete growth. The present findings demonstrated that the interactive effects of hypoxia and environmentally realistic concentrations of BDE-47 in sediments could affect polychaete settlement, which, in turn, reduce its recruitment and subsequent population size in the marine benthic ecosystem.

Detecting benthic community responses to pollution in estuaries: A field mesocosm approach

Biological stress responses in individuals are used as indicators of pollution in aquatic ecosystems, but detecting ecologically relevant responses in whole communities remains a challenge. We developed an experimental approach to detect the effects of pollution on estuarine communities using field-based mesocosms. Mesocosms containing defaunated sediments from four estuaries in southeastern Australia that varied in sediment contamination were transplanted and buried in sediments of the same four estuaries for six weeks. Mesocosm sediment properties and metal concentrations remained representative of their source locations. In each estuary, fauna communities associated with sediments derived from the site with the highest metal concentrations were significantly different from other communities. This pattern was evident for some of the individual taxa, in particular the polychaete Capitella sp. Consistent responses across estuaries suggest numbers of individuals, and especially Capitella sp., could be used to identify contaminated sediments in estuaries with similar fauna and site characteristics.

Differential expression of proteins and phosphoproteins during larval metamorphosis of the polychaete Capitella sp. I

BackgroundThe spontaneous metamorphosis of the polychaete Capitella sp. I larvae into juveniles requires minor morphological changes, including segment formation, body elongation, and loss of cilia. In this study, we investigated changes in the expression patterns of both proteins and phosphoproteins during the transition from larvae to juveniles in this species. We used two-dimensional gel electrophoresis (2-DE) followed by multiplex fluorescent staining and MALDI-TOF mass spectrometry analysis to identify the differentially expressed proteins as well as the protein and phosphoprotein profiles of both competent larvae and juveniles.ResultsTwenty-three differentially expressed proteins were identified in the two developmental stages. Expression patterns of two of those proteins were examined at the protein level by Western blot analysis while seven were further studied at the mRNA level by real-time PCR. Results showed that proteins related to cell division, cell migration, energy storage and oxidative stress were plentifully expressed in the competent larvae; in contrast, proteins involved in oxidative metabolism and transcriptional regulation were abundantly expressed in the juveniles.ConclusionIt is likely that these differentially expressed proteins are involved in regulating the larval metamorphosis process and can be used as protein markers for studying molecular mechanisms associated with larval metamorphosis in polychaetes.

Macrobenthic community structure in a Brazilian chocked lagoon system under environmental stress

Saquarema-Jaconé lagoonal system (SJLS) comprises a sequence of five interconnected shallow brackish lagoons with access to the sea by a single permanent tidal channel. It is a eutrophic system, receiving constant input of organic load from its urbanized catchments. The relationship between several environmental variables and the spatial-temporal distribution of the benthic macrofauna was assessed during four seasonal samplings (dry and wet periods of 2007-2009). Sediment replicates were sampled at seven sites for biological identification and analyzes of organic matter, carbonates, phytopigments, grain size and heavy metals. Salinity, dissolved oxygen and redox potential were measured in situ. SJLS was characterized by sandy bottoms with very reducing conditions. Redox potential significantly discriminated between the dry and wet periods and anoxic conditions were observed in the latter. No significant seasonal differences were observed in the macrofauna. A total of 37 taxa were identified, of which Capitella sp, oligochaetes and Laeonereis culveri (Webster, 1880) were the dominant, representing the early stage of community recovery following dystrophic crises. The faunistic pattern seems to be determined by complex combinations of silt+clay with salinity, organic matter and redox potential. On the other hand, the low concentrations of heavy metals found did not seem to influence the structure and distribution of the biota. SJLS is undergoing persistent environmental stress, dominated by first-order opportunistic species linked to organically enriched sediments.

Increase in Alphaproteobacteria in association with a polychaete, Capitella sp. I, in the organically enriched sediment

We conducted bioremediation experiments on the organically enriched sediment on the sea floor just below a fish farm, introducing artificially mass-cultured colonies of deposit-feeding polychaete, Capitella sp. I. To clarify the association between the Capitella and bacteria on the efficient decomposition of the organic matter in the sediment in the experiments, we tried to identify the bacteria that increased in the microbial community in the sediment with dense patches of the Capitella. The relationship between TOC and quinone content of the sediment as an indicator of the bacterial abundance was not clear, while a significant positive correlation was found between Capitella biomass and quinone content of the sediment. In particular, ubiquinone-10, which is present in members of the class Alphaproteobacteria, increased in the sediment with dense patches of the Capitella. We performed denaturing gradient gel electrophoresis (DGGE) analyses to identify the alphaproteobacterial species in the sediment with dense patches of the worm, using two DGGE fragments obtained from the sediment samples and one fragment from the worm body. The sequences of these DGGE fragments were closely related to the specific members of the Roseobacter clade. In the associated system with the Capitella and the bacteria in the organically enriched sediment, the decomposition of the organic matter may proceed rapidly. It is very likely that the Capitella works as a promoter of bacteria in the organically enriched sediment, and feeds the increased bacteria as one of the main foods, while the bacteria decompose the organic matter in the sediment with the assistance of the Capitella.

Somatic and germline expression of piwi during development and regeneration in the marine polychaete annelid Capitella teleta

BackgroundStem cells have a critical role during adult growth and regeneration. Germline stem cells are specialized stem cells that produce gametes during sexual reproduction. Capitella teleta (formerly Capitella sp. I) is a polychaete annelid that reproduces sexually, exhibits adult growth and regeneration, and thus, is a good model to study the relationship between somatic and germline stem cells.ResultsWe characterize expression of the two C. teleta orthologs of piwi, genes with roles in germline development in diverse organisms. Ct-piwi1 and Ct-piwi2 are expressed throughout the life cycle in a dynamic pattern that includes both somatic and germline cells, and show nearly identical expression patterns at all stages examined. Both genes are broadly expressed during embryonic and larval development, gradually becoming restricted to putative primordial germ cells (PGCs) and the posterior growth zone. In juveniles, Ct-piwi1 is expressed in the presumptive gonads, and in reproductive adults, it is detected in gonads and the posterior growth zone. In addition, Ct-piwi1 is expressed in a population of putative PGCs that persist in sexually mature adults, likely in a stem cell niche. Ct-piwi1 is expressed in regenerating tissue, and once segments differentiate, it becomes most prominent in the posterior growth zone and immature oocytes in regenerating ovaries of regenerating segments.ConclusionsIn C. teleta, piwi genes may have retained an ancestral role as genetic regulators of both somatic and germline stem cells. It is likely that piwi genes, and associated stem cell co-regulators, became restricted to the germline in some taxa during the course of evolution.