Lepus Capensis Research Articles

A chromosome-level genome assembly of Cape hare (Lepus capensis)

The Cape hare (Lepus capensis) is among the most widely distributed hare species globally, inhabiting extensive regions across Africa, the Middle East, and Central Asia. However, evolutionary and genetic research on L. capensis was seriously impeded by the absence of a reference genome. Here, we assembled and constructed a chromosome-level genome of L. capensis (with scaffolds anchored to 25 chromosomes and a total assembled length of 2.9 Gb, achieving a contig N50 length of 124.44 Mb) using PacBio HiFi sequencing and Hi-C assembly technology. Evaluation using BUSCO indicated the genome assembly to be 98.2% complete. The de novo prediction revealed that repetitive sequences constitute 46.13% of the entire genome, and long interspersed nuclear elements (LINEs) constituted the largest portion. We annotated a total of 13, 868 protein-coding genes using transcriptomes from two tissues (muscle and skin). This high-quality reference genome serves as a valuable genomic resource for advancing genetic studies in this species.

Safeguarding the last stronghold: Ecology and conservation of Asiatic Cheetah's prey species in Turan Biosphere Reserve (Iran)

Identifying suitable habitats for wild felids is essential for effective area-based conservation efforts. However, modeling habitat suitability for extremely rare and endangered taxa such as the Asiatic Cheetah (Acinonyx jubatus venaticus) is challenging due to limited data availability. To address this, our study focused on analysing the distribution factors of the Asiatic Cheetah's main prey species, including the Wild Goat (Capra aegagrus), Urial (Ovis vignei), Goitered Gazelle (Gazella subgutturosa), Jebeer Gazelle (Gazella bennettii), and Cape Hare (Lepus capensis) in the Turan Biosphere Reserve (TBR), a critical Cheetah habitat in Iran. To determine habitat suitability for these prey species, we conducted a systematic field survey along 30 transects measuring 16 km each by collecting data in each 1 km interval, resulting in 1172 direct observations of their presence. These observations were then correlated with 44 variables grouped into four categories: bioclimatic, topography, vegetation, and distance to anthropogenic and natural features. Suitability maps were generated using a hierarchical modeling approach. Among the observed prey species, Urial had the highest frequency of sightings (675 visual observations over eight seasons), followed by Goitered Gazelle (564 observations), Cape Hare (278 observations), Jebeer Gazelle (232 observations), and Wild Goat (208 observations). The most influential predictors affecting prey species distribution were mean summer temperature (with optimal ranges at lower temperatures), terrain relief roughness (with optimal ranges at intermediate roughness), distance to wells (with optimal ranges at shorter distances), and distance to corrals (with variable optimal ranges across species). Our findings indicate that the western and eastern mountainous areas, along with their hilly surroundings, as well as the northwest and western plains of TBR, constitute the most suitable habitats for these five prey species. These models and suitability maps provide crucial evidence for implementing conservation measures in the vulnerable habitats of the Asiatic Cheetah and its primary prey species. Environmental conditions can be enhanced through the management of water sources, roads, corrals, and livestock grazing. In addition, the fragmentation of suitable areas within TBR highlights the importance of preserving corridors between these fragmented habitats. We recommend re-evaluating and expanding the boundaries of Turan National Park, Turan Wildlife Refuge, and Turan Biosphere Reserve to ensure the long-term conservation of this threatened felid species and its associated ecosystems.

Phylogenetic status and diet of red fox (Vulpes vulpes griffithii) inhabiting Ayubia National Park, Pakistan

Abstract The red fox (Vulpes vulpes) is a medium-sized carnivore that occurs in different regions of Pakistan, however, still lacks scientific data on its ecology and distribution. The current study investigated the phylogenetic status and diet of the red fox (V.v. griffithii) occurring in Ayubia National Park, Pakistan. Through camera trapping and molecular analysis, we confirmed the occurrence of red fox in the study area. Based on mitochondrial cytochrome B (304 bp) and limited sampling, nearly all red foxes of Ayubia National Park and surrounding Himalayan ranges fall within Holarctic maternal lineage, whereas red foxes found in plains of Pakistan are part of the basal Palearctic maternal lineage. Using 32 scats, we found that red fox diet comprises of 80% animal-based prey species (both wild and domestic) and 19% plant matter. The wild animal prey species included Cape hare (Lepus capensis) and flying squirrel (Pteromyini sp.), which constituted 17% and 15% of diet, respectively. Red foxes infrequently consumed House mouse (Mus musculus), Himalayan Palm civet (Paguma larvata) and sheep (Ovis aries), each comprising around 6% to 9% of red fox diet. The fox species also scavenged on domestic donkey opportunistically. Based on our sampling, our study suggests that the red fox (V.v. griffithii) that occurs in Ayubia National Park and across the lesser Himalayan ranges belongs to Holarctic maternal lineage. The study also highlights consumption of plant seeds by red foxes, indicating it may play an important ecological role in seed dispersal in Ayubia National Park.

Estrogen regulated transcription of the non-estrogen-regulated hamster uteroglobin gene in MCF-7 cells

To study the estrogen regulated transcription of the uteroglobin (UG) gene, the founding member of the secretoglobin family widely expressed in many different mammalian species, we re-created functional estrogen response elements (EREs) in the UG gene promoter from a species where UG expression is not regulated by estrogens: the hamster Mesocricetus auratus (Ma), to ascertain if the lack of functional EREs is the real cause of its estrogen insensitivity. Functional EREs in the hamster promoter, including the consensus ERE (cERE), failed to respond to an appropriate estrogen stimulus compared with its estrogen regulated ortholog from the brown hare Lepus capensis (Lc). As the nucleotide sequence is the only difference between genetic constructs from these two species, we suspected that the UG promoter from the hamster probably contains cis-acting genetic elements that negatively impairs the estrogen-regulated transcription mediated by the functional ERE. Accordingly, we prepared chimeric DNA constructs which eventually allowed to identify a region located 29 base pairs (bp) downstream of the ERE as responsible for the lack of estrogen-responsiveness of the Ma-UG gene in the breast cancer cell line MCF-7. This region contains the sequence ACACCCC which has been identified as the core sequence of the Sp/ Krüppel-like factor (KLF) family of transcription factors. This finding is relevant, not only due to the observation on a novel mechanism that control estrogen-induced transcription, but also because it may encourage further investigation for better defining specific genes with an ERE that do not respond to estrogen signaling in MCF-7 cells, a cell line widely employed as an in vitro model in breast cancer research.

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The skull is one of the largest bones in the body. It is classified into flat bones that maintain the important organic structures; which are the brain, eyes, and tongue. The skull is a strong support for preserving these organs but they are various according to the type of animals and the environments in which they live and the nature of their nutrition. There are many differences among living organisms in terms of the bones in the skull, their difference or disappearance and their length in the shape of the head. The samples were taken from the scientific storage in the Iraq Natural History Research Center and Museum; Cape hare Lepus capensis (Linnaeus, 1758) and Red fox Vulpes vulpes (Linnaeus, 1758) and the study was conducted on them in a comparative morpho-anatomical way; it is noted that it differs from one animal to another. The dentition formula was added because the dental tissue is embedded in the jaw bone. Differences were noted in the current study in comparison to other previous studies. However the study of bones needs development in methods and requires an extensive investigations in Iraq as a result of the dissimilarities in species and the nature of living; in addition to the species itself, differences are registered.

Diversity and Density of Small Mammal Species with Different Seasons and Habitats in Federal University Gashua, Yobe State, North-east Zone of Nigeria

The study examined diversity and density of small mammal species with different seasons and habitats in Federal University Gashua, Yobe State, North-East, Nigeria. The university environment was divided in to four (4) habitat types, namely; University residential area (URA), Acacia woodlot (AWL), Lake Area (LA) and Farm Land (LF) were used for the study. Small Mammal species survey was carried out for the period of six (6) months from August to October, 2022 (wet season) and January, to March, 2023 (dry season). Capture Mark–Recapture (CMR) and indirect observations methods were adopted; Survey was conducted using spring door traps of different sizes set at the habitat types during late evening hours 6:00 pm and checked in the morning hours from 6:00-8:30am for four (4) days per week with each habitat types being visited one day per week, making total of (96) man-days. Each habitat type was visited twice a day (morning and evening) which gave a total of (65;60) trapping night in both wet and dry seasons, the number decreased to 60 trapping night in the dry season was due to the wearing a way of vegetation cover. (32) Visits per habitat types and one (1) night survey per month. A total of ten (10) species of small mammals belonging to ten (10) families and seven (7) orders were recorded in the study. African Hedgehog Atelerix albiventris and White toothed shrew Crocidura flavescens were the most occurring species across The findings of the study shows that the overall diversity index was (1.692), where Four-toed hedgehog Atelerix albiventris had the highest species diversity90.363) and estimated population density of (64.44) followed by Giant pouched rat Cricetomys gambianus with (0.277; 0.018) respectively, Cape hare Lepus capensis and Mongoose Herpestes ichneumon who’s having the lowest diversity of (0.017) with (0. 564) density each in the study area. The month of February has higher number of captured percentage with (24%) in dry season, followed by October with (19%) in the wet season, the lowest capture percentage occurred with August (14%) in the wet season. University Residential Area had more number of individual species having (37.82 %), while Farm land had the least captured percentage of(16.85%).The trap success in both wet season and dry season’s period were (33.04%; 21.67%)respectively. Habitat alteration and destruction due to ongoing constructions project, mainly for university deployment might have accounted for small mammal species low diversity and density in Federal University Gashua.

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Ecological ApplicationsVolume 33, Issue 3 e2659 COVER IMAGEFree Access Cover Image First published: 03 April 2023 https://doi.org/10.1002/eap.2659AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Graphical Abstract COVER PHOTO: Pictured is a Cape hare (Lepus capensis) photographed in 2018 in Wadi Tznifim within the Nehalim Gdolim Nature Reserve, situated in the southern Negev Desert and Arava mountains of Israel. The Cape hare is native to the region. In their study published in this issue, Lewin et al. (Article e2804; doi:10.1002/eap.2804) show that nearby intensive agroecosystems have impacted these and other native desert species, as well as invasives, through added agricultural resources mediated by trophic cascades. Photo credit: Doron Nissim. Volume33, Issue3April 2023e2659 RelatedInformation

Francisella tularensis PCR detection in Cape hares (Lepus capensis) and wild rabbits (Oryctolagus cuniculus) in Algeria

Tularemia is a zoonosis caused by the bacterium Francisella tularensis. Leporids are primary sources of human infections in the northern hemisphere. Africa is classically considered free of tularemia, but recent data indicate that this dogma might be wrong. We assessed the presence of this disease in wild leporids in Algeria. Between 2014 and 2018, we collected 74 leporids carcasses from spontaneously dead or hunted animals. Francisella tularensis DNA was detected by specific real-time PCR tests in 7/36 (19.44%) Cape hares (Lepus capensis) and 5/38 (13.15%) wild rabbits (Oryctolagus cuniculus). Known tularemia arthropod vectors infested half of the PCR-positive animals. At necropsy, F. tularensis-infected animals presented with an enlarged spleen (n = 12), enlarged adrenal glands (12), liver discoloration (12), hemorrhages (11), and pneumonia (11). Immunohistological examination of liver tissue from one animal was compatible with the presence of F. tularensis. Our study demonstrates the existence of tularemia in lagomorphs in Algeria. It should encourage investigations to detect this disease among the human population of this country.

Green and blue infrastructure design in a semi-arid region

The Green and Blue Infrastructure (GBI) is a new approach for dealing with biodiversity conservation based on maintaining the ecological networks by ensuring the survival of animal and plant species. GBI is considered a new public policy to avoid the loss of biodiversity by preserving landscape connectivity and protecting natural habitats from fragmentation. Countries including the United States, Canada, the United Kingdom, Italy, Portugal, Japan, China, Brazil, and Turkey are seeking to integrate this concept into their environmental policies. This study proposed a methodology to design a GBI at a regional scale applied to the Sousse region in Tunisia. Two main approaches were used. The first was based on identifying three reference species marked by different dispersal distances. The second applied GIS spatial analysis using the buffer model to help assess the ecological connectivity. The methodology developed comprises five steps. In the first step, a forest habitat sub-frame, a semi-open habitat sub-frame, and a wetland sub-frame were determined from a detailed land-use map. Then, different biodiversity reservoirs were identified. In the third step, buffers varying between 200, 250, and 300 m depending on the dispersal distance of the reference species (Cape hare, North African Hedgehog, and Marbled Teal) were applied around the reservoirs to identify zones of potential connections between them. Next, buffers were also applied to detect fragmentation zones along road networks and built-up areas. Finally, the ecological corridors were mapped from the overlay between zones of potential connections and the fragmentation map. The result was the creation of a GBI of the Sousse region with reservoirs of biodiversity and ecological corridors connecting them. This methodological approach and the characterization of the Sousse city GBI map represent the first innovative research applied in Tunisia. This prototype can be a reference for other local or regional spaces, contributing toward realizing a national GBI and promoting sustainable projects.

Assessing Asiatic cheetah’s individual diet using metabarcoding and its implication for conservation

Knowledge on diet composition allows defining well-targeted conservation measures of large carnivores. Little is known about ecology of critically endangered Asiatic cheetah, especially the overall diet and its possible regional differences. We used cheetah scats, metabarcoding technique and microsatellite markers to assess the individual and overall diet composition of the species across its entire range in Asia. Cheetahs were primarily predating on mouflon; following by ibex, cape hare and goitered gazelle. Despite their high availability, small-sized livestock was never detected. Goitered gazelles were only detected in an area where the habitat is mainly flatlands. In hilly areas, mouflon was the most frequent prey item taken. Ibex was typically taken in rugged terrain, but mouflon was still the most frequently consumed item in these habitats. High consumption of mouflon in comparison to goitered gazelle suggests that human pressure on lowland habitats has possibly forced Asiatic cheetahs to occupy suboptimal habitats where gazelles are less abundant. The protection of flatlands and the removal of livestock from them are needed to ensure the long-term survival of Asiatic cheetah. The laboratory and bioinformatics pipelines used in this study are replicable and can be used to address similar questions in other threatened carnivores.

Spatial diversity of MHC class II DRB exon2 sequences in North African cape hares (Lepus capensis): positive selection and climatic adaptation signals

Spatial diversity of MHC class II DRB exon2 sequences in North African cape hares (Lepus capensis): positive selection and climatic adaptation signals

Histomorphological and histochemical study of the fallopian tube during pregnancy and post-partum of the cape hare rabbit (Lepus Capensis)

This research was investigate the Anatomical and Histochemical structure of uterine tube during period of pregnancy and postpartum in cap hare rabbits. Eighteen female rabbits were used. The target samples were processing with paraffin embedding technique the tissue sections stained with H&E stain, and Masson's trichrom stain. Results; The length and weight of the uterine tube were (91.31 ± 0.72mm), (0.53 ±0.01g) at pregnancy. While. its (84.76 ±0.22mm), (0.41 ±0.01g) at post-partum. The infundibulum has a funnel-shaped end. Simple mucosal folds are bordered with pseudo stratified columnar epithelium. The lamina propriea-sub-mucosa is made up of connective tissue that is both vascular and cellular. The ampulla was highly vascularized and had a twisted appearance. The mucosal folds of the ampulla were lined by pseudo stratified columnar epithelium, which consisted of three cell types: mucous secretory cells, non-secretory cells and basal cells. The lamina propria was a thin layer of fibrous connective tissue that exposed a significant amount of fibroblast. The isthmus was short and straight at post-partum, and it was held in place by the mesosalpinx. Mucosal folds in the tunica mucosa are mild and short.

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Roads that traverse through protected areas if not well managed can have adverse impact on wildlife such as road-kills which is of global conservation concern. Though mammalian road-kills have been reported in different protected areas worldwide, very little information on the problem is available in the Serengeti ecosystem. This study employed both cross sectional observation and opportunistic encounter methods to determine the patterns of mammalian road-kills along the existing gravel road networks in the area. The results indicated that 29 mammals with encounter rates of 0.016 animals/kilometer including herbivores (75.9%), carnivores (13.8%) and omnivores (10.3%) were found killed more frequently on good roads, probably because of over speeding behavior by drivers. Mammals with small body sizes (<10.0 kg, 44.8%) predominantly Cape hares (Lepus capensis, 31.0%) and Thomson gazelle (Eudorcas thomsonii, 27.6%) were most frequently killed probably because they are less avoided by motorists than larger mammals; and also, smaller mammals move slowly in crossing the roads than larger mammals, which increases the chances of being hit by vehicles. Cape hares and Thomson gazelles are more abundant species in the Serengeti and their behavior of foraging on road verges and frequently crossing roads to access resources in the area is additional risk. The study findings recommend for high penalties to over speeding drivers and placing wildlife warning signs on the roadside, and education to drivers to change behaviour and reduce road-kills.   Key words: Small body-size, impact, mammals, protected areas, roads, tourism.

THE DIET OF THE TUCÚQUERE (BUBO VIRGINIANUS MAGELLANICUS) IN ANDEAN CENTRAL CHILE

The tucúquere (Bubo virginianus magellanicus) is a bird of prey with an agro-ecological and environmental relevance in Chile. However, there is a gap about the composition of the diet of B. v. magellanicus in mountainous regions of central Chile, where interaction with the human population is large and anthropic derived habitat changes are increasing. Ninety-one regurgitated pellets from two birds were collected. Analysis of pellet weight, length and width and prey characterisation to the species level, absolute and relative abundance were obtained. Trophic niche (Levin Shannon-Wiener and Smith index) were calculated. The average weight, length and width of pellets were 9.84 g, 5.67 cm and 3.26 mm, respectively. A consumption of 12 different species of prey was evidenced, being the most consumed Lepus capensis (23.53%), followed by Abrocoma bennetti (20.59%) and then other species of small rodents, and an undetermined bird and insect. The trophic niche amplitude for normal and standardised Levin, normal and standardised Shannon-Wiener and Smith (TF) indexes were 6.72, 0.52, 2.11, 0.85 and 0.90, respectively. This is the first evidence of a diet based mainly on Lepus capensis in central Chile for B. v. magellanicus, who seems to be a more generalist predator than previous studies. The results are of significance for the understanding of feeding habits and conservation efforts for raptor species in Chile and South America.

Epidemiological study and identification of some flea species infesting wild rabbits (Oryctolagus cuniculus) and cape hares (Lepus capensis) in northern Algeria

Wild rabbit (Oryctolagus cuniculus) and cape hare (Lepus capensis) are hosts for different flea species and have high medical and veterinary importance, fleas of wild leporids are of special concern since they can act as vectors for diseases, including zoonoses. This study was designed to identify flea species infesting these animals with the description of their epidemiology and the assessment of factors influencing their infestation importance in five provinces located in northern Algeria, to evaluate their implication in the transmission of pathogens. In total, 86 wild leporids were checked from July 2014 to September 2020. Fleas were collected from the fur of animals with tweezers and identified using appropriate keys. The findings showed that 32/86 (37.2%) of animals were infested with fleas. Four flea’s species were identified on cape hares Ctenocefalides felis (51.06% of fleas identified), Ctenocefalides canis 34.04%, Spilopsyllus cuniculi 10.63%, and Archaeopsylla erinacei 4.25%,with a seasonal peak in December. The same, four flea species were found parasitizing wild rabbits Spilopsyllus cuniculi 90.7%, Ctenocefalides felis 4.65%, Ctenocefalides canis 2.32%, and Pulex irritans 2.32%,with the highest importance in March and April. Moreover, this study showed no influence of female gestations on the rate of flea hare infestations. However, S cuniculi rate was correlated with the breeding cycle of the wild rabbits. These findings revealed that, the temperature and the humidity can be important factors that influence on wild leporids flea infestation.

A large mammal survey in Koyli Alpha Community Wildlife Reserve and its surroundings in the Great Green Wall extension area in Senegal

Wildlife in the Great Green Wall (GGW) area, northern Senegal, is threatened by the cumulative effects of anthropogenic activities, drought, and climatic changes. To support conservation planning in line with the GGW management objectives, we studied large mammal diversity in the Koyli Alpha Community Wildlife Reserve in the GGW extension area. We interviewed local communities, made reconnaissance & transect walks, placed camera traps, and carried out fixed point surveys to map the distribution of mammals & to estimate their relative abundance. The surveys were conducted between May 2017 and December 2019. We identified the presence of nine mammal species belonging to six families: one lagomorph, Cape Hare Lepus capensis; seven carnivores, Common Jackal Canis aureus, Pale/Sand Fox Vulpes pallida, Wild Cat Felis silvestris, Marsh Mongoose Atilax paludinosus, Honey Badger Mellivora capensis, Zorilla Ictonyx striatus, Common Genet Genetta genetta, and one primate, Patas Monkey Erythrocebus patas. Our results indicate that the most of the observed species range broadly across the Koyli Alpha Reserve. The Wild Cat and the Sand Fox were also found outside the reserve around the village of Koyli Alpha. Camera trapping events of humans and domestic animals were high throughout the survey area, and the majority of the large mammals observed were mainly nocturnal. The diversity of large wild mammals identified during this study points to the urgent need for the implementation of a management plan of the biodiversity in this area of the Ferlo.

Magnitude, patterns and composition of wildlife roadkill in the Serengeti ecosystem, northern Tanzania

Globally, traffic associated with roads that pass through protected areas are the cause of many negative impacts on wildlife, such as wildlife roadkill, which has occurred within the Serengeti ecosystem. A 200 km stretch of gravel road networks that passes through the Serengeti ecosystem was surveyed in 2015 for five consecutive days during each study period during the wet and dry seasons, between March and August 2015, to document the magnitude, patterns and composition of wildlife roadkill. We found a total of 51 wildlife roadkills that consisted of 26 species of wild animals and included 18 bird and 8 mammal species. Bird species (60.8%) were more frequently killed than mammal species (39.2%). Moreover, a higher incidence of wildlife roadkill was recorded in the morning (56.9%) than in the afternoon (43.1%) and more mammals (51.7%) than birds (48.3%) were recorded in the morning. Greater numbers of wildlife roadkill were recorded along road segments with good road conditions (69.4%) that were wider (>8 m) and with higher traffic volumes than along roads with poor conditions (30.6%). Overall, the Cape hare (Lepus capensis) was the most frequently killed species (17.6%) among the mammals, whereas the helmeted guineafowl (Numida meleagris) (13.7%) was most frequently killed among the avifauna. To reduce the number of roadkill incidents, we recommend changing driver speeding behaviours by installing wildlife-warning signage that consists of picture-based signs, because they have been proved to be more effective than word-based signs to reduce speeds and hence, reduce collisions. Additionally, the responsible management authorities should provide education to drivers on safe driving and enforcement of laws and regulations. Further, policy briefings that focus on wildlife roadkill should be made available to assist decision-makers and engineers to improve road design in protected areas.

On the Hunting of, and Threats Facing, the Cape Hare (Lepus capensis Linnaeus, 1758) in the Gaza Strip, Palestine

The Cape Hare (Lepus capensis Linnaeus, 1758) is the only lagomorph in Palestine (27,000 km2). Although it was common in the Gaza Strip 4-5 decades ago, its present occurrence is restricted by several overlapping factors. The current study aims to spotlight the hunting of, and the threats facing, Cape Hares in the Gaza Strip (365 km2). This descriptive study, which lasted 5 years (2016 – 2020), was based on frequent field visits and observations in addition to meetings and discussions with wildlife hunters and farmers. We found that the eastern parts of the Gaza Strip were the main hunting places for Cape Hares. The methods involved in hare hunting included shooting, foothold traps, live traps known locally as "maltash", net walls, pit traps and coursing which involves the use of greyhounds. Wildlife hunters described the hunting of Cape Hares as a complicated task due to its low occurrence, extreme caution, difficulty of tracking it, presence near the Israeli security fence, which poses a danger to hunters, in addition to the great experience and high concentration that such hunting requires. Cape Hares face several threats, some of which are Israeli, and some specific to Gazans and the environment of the Gaza Strip. The Israeli threats include the Israeli security fence which prevents the natural flow of wildlife between the Gaza Strip and the rest of the Palestinian territories, Israeli wars and invasions, and the Israeli spraying of herbicides at the eastern border areas of the Gaza Strip for claimed security reasons which eradicates the plant cover supporting hares and other fauna with shelter and food. The Gazan threats include urban encroachment at the expense of natural habitats, excessive use of chemical pesticides, overhunting of Cape Hares, noticeable increase of opportunistic carnivores feeding on Cape Hares, and weakness of environmental awareness campaigns and laws that protect wildlife. Finally, the study recommends the use of all possible means to sustainably protect and conserve wildlife including Cape Hares in the Gaza Strip.

Protozoa and Helminth Parasitic Fauna of Wild Animals in Daquq District, Kirkuk Province, Iraq.

In the current study, a number of wild animals were collected, examined, and dissected, and the following types of parasites were found: The parasitic protozoan: the genus Haemoproteus, and this is the first record of this genus in the lizard Uromastyx loricata. The genus Eimeria was found in the rabbit Lepus capensis and the turtle Mauremys caspica, while Entamoeba was found in the pig Sus sucrofa. The tapeworm’s genus Anoplocephala in hedgehog Hemiechinus auritus, which is a "new" host for this worm, while three genera were isolated for the first time from the fox, Vulpes vuIpes which are Fossor angertrudae, Pericapsifer pagenstecheri, and Cycloskrjabinia taborensis. The nematode genera Rhaphidascaris acus and Cosmocerca trispinosa were found in the turtle Mauremys caspica. The Acanthocephalas theGenus AcanthocephaIa was isolated from the hedgehog Hemiechinus auritus for the first time.

Hare today, gone tomorrow: the role of interspecific competition in shaping riverine rabbit occurrence

Effective conservation, particularly of threatened species, requires an understanding of both abiotic and biotic drivers of distribution. In the case of one of Africa’s most endangered mammals, the riverine rabbit Bunolagus monticularis, only environmental covariates of presence have been used to provide coarse predictions of their distribution. Two potential competitors, namely scrub hare Lepus saxatilis and cape hare L. capensis, have significant (>90%) range overlap with the riverine rabbit, yet little is known about how these species interact. We used multi-species occupancy models, which model co-occurrence as a function of environmental variables, to assess the spatial response of riverine rabbits to both species of hare in Sanbona Wildlife Reserve, South Africa. We also examined temporal overlap between riverine rabbits and hares. Camera trapping data were collected from 150 camera traps distributed in clusters of 5 cameras at 30 independent sites, covering 223.24 km2. Contrary to prior studies, we found that riverine rabbits were not restricted to riparian habitat, and that their occurrence was conditional on hare absence and was negatively affected by terrain ruggedness. Whilst hare occurrence was independent of terrain ruggedness, it was negatively affected by rabbit presence. Activity patterns revealed high temporal overlap between hares and rabbits (Δ = 0.828, CI = 0.745-0.940); however, neither species co-occurred at any given site. Our results suggest that conservation management has greatly underestimated the importance of competition with other lagomorphs in understanding riverine rabbit occurrence.