We present ostracod data from the Middle Palaeolithic open air site of Nahal Mahanayeem Outlet (NMO) at the southern edge of the Hula Basin, northern Israel.Sediments of two Pleistocene water bodies are exposed at the site. The first one is an archaeologically sterile, light-colored limnic carbonate with an Early Pleistocene age. It contains an ostracod fauna assemblage dominated by Candona neglecta, Candonopsis kingsleii, and Pseudocandona sp., and, in minor abundances, Cypria ophtalmica, Cyprideis sp., Humphcypris sp., Fabaeformiscandona cf. fabaeformis and Ilyocypris sp. These sediments were deposited in a shallow, freshwater to oligohaline lake under stable conditions.Sediments of the second water body are silty and dark-colored with a depositional age of 65 ka, belonging to the Late Pleistocene Ashmura Formation. The unit covers a geologically complex topography of tectonically uplifted limnic deposits and a hill-like gravel bar at the site. The most important archaeological layer is situated at its base, containing a lithic assemblage ascribed primarily to the Middle Palaeolithic Mousterian tradition and very well preserved flora and fauna. In the sediments from the archaeological layers, the brackish water ostracod Cyprideis torosa and the foraminifer Ammonia tepida could be identified. In sediments of the Ashmura Formation taken near the site, C. neglecta, Candona angulata, Ilyocypris sp., C. kingsleii, Pseudocandona sp., C. ophtalmica, Darwinula stevensoni, Trajancypris sp. and Potamocypris smaragdina were found, indicating a freshwater to slightly oligohaline stagnant water body.The ostracod fauna of the NMO site, together with geochemical data, allow us to reconstruct a depositional environment of the margin at a shallow lake with brackish or saline springs nearby during the site's occupation by Middle Palaeolithic humans.Additionally, Candona weltneri, Candona cf. meerfeldiana, C. kingsleii, Cyclocypris laevis, C. ophtalmica, Cyprideis sp., Fabaeformiscandona cf. fabaeformis, P. smaragdina, Pseudocandona depressa, Trajancypris sp, Zonocypris cf. costata and A. tepida could be recorded for the first time for the Pleistocene limnic strata of the Hula Basin.
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