Candida Albicans In Patients Research Articles

Fungal Infection of the Oral Cavity by Candida Albicans in Patients Wearing a Removable Dental Prosthesis

Permanent wearing of a removable prosthesis can be a source of mechanical irritation, which will lead to the appearance of pathological conditions. These conditions can be of several types but the most common are oral candidiasis with Candida albicans. As part of the scientific research, we have begun a practical study that aims to establish the relationship between the wearing of a removable denture and the colonization of the oral cavity by Candida albicans. This study was carried out in collaboration with the central laboratory of parasitology –mycology of the Ibn Sina University Hospital Center of Rabat (CHU) and assistant prosthesis department of the clinic of the Faculty of Dentistry of Rabat. The present study was carried out on 45 specimens from the oral cavity in subjects with a removable prosthesis. We observed that most subjects affected by oral candidiasis had inflammation in the oral cavity and these subjects did not have adequate oral hygiene. 30% of these subjects had chronic diseases and 20% of male subjects were smokers. Most subjects with prostheses older than 10 years were the most affected. Mycological examination of the samples made allowed us to note 35.5% of fungal infection by the genus Candida and 27% by the species Candida Albicans. C. albicans was the most isolated yeast representing 75% of the yeasts isolated in this study, followed by C. famata, after C.tropicalis with a lower frequency and finally C.dubluensis with the lowest frequency. We also observed a relationship between the nocturnal use of the prosthesis and the presence of Candida albicans, indeed most subjects affected by oral candidiasis kept their prostheses during the night.

Activation of CD4+CD25+CD45+ T-lymphocytes by Candida albicans in vitro in patients with leukoplakia associated with candidal stomatitis

The mechanisms and patterns of T-lymphocyte activation in response to the effects of Candida albicans are currently insufficiently studied. Therefore, the study of of lymphocytes expressing CD4, CD25, CD45 against the background of precancer and cancer of the oral mucosa is very relevant. The purpose of the study was to study the in vitro effect of Candida albicans on the expression of CD4+CD25+CD45+ T-lymphocytes in the blood of patients with candidal stomatitis with and without leukoplakia. Material and methods. The study involved 93 patients with candidal stomatitis (with and without leukoplakia) aged from 36 to 79 years with chronic and recurrent candidiasis. 30 patients with leukoplakia without candidal stomatitis aged from 38 to 71 years old. The control group consisted of 30 patients aged from 34 to 75 years without candidal stomatitis and leukoplakia. Leukocytes from peripheral blood patients were incubated for 24 hours at 37°C with 2.5 μl of Candida albicans (experimental sample) and with 2.5 μl of buffered saline (control sample). The cells were then phenotyped with CD4+CD25+CD45+ monoclonal antibodies. Conclusions. In the control sample, CD25 expression was higher in the groups of patients with candidiasis (with and without leukoplakia). The expression of activation markers – CD25+-increased on patient lymphocytes in the experimental sample. There were more activated T lymphocytes in response to stimulation by fungi of the genus Candida albicans (p<0.05), compared to lymphocytes in the control sample. The main increase was observed in the group of patients with leukoplakia, candidal stomatitis and endothelial dysplasia, in which Treg lymphocytes were determined. These data confirm the presence of a pronounced response of CD4+ T lymphocytes to fungi of the genus Candida albicans in patients with candidal stomatitis.

Ophthalmia " Adultorum" Secondary to Cytomegalovirus and Candida albicans Isolates from Right Eye Ulcer of an Alcoholic: The Benin City Experience

Background: Eye injuries are more common and have more severe effects when they occur in developing countries. The factors reponsible for these eye injuries can be linked to socioeconomic status, insufficient safety precautions, a lack of ideal treatment facilities, the use of conventional eye medication, and low levels of education. Ocular trauma has been identified in studies from Nigeria and other African countries as a major contributing factor to monocular blindness.
 In neonates, conjunctivitis within the first 28 days of life is termed “Ophthalmia neonatorium”. Conjunctivitis arising from either viral, bacteria or fungal causes in an Adult is what we termed as “Ophthalmia Adultorum".
 In our study the individual was a chronic alcoholic who was already immunosuppressed from his large alcohol intake. This study therefore is case report of a traumatic innoculation causing Ophthalmia Adultorum in a male alcoholic, a university of Benin Teaching Hospital experience.
 Methods: This is a case report.
 Results: A 57 year old man who was admitted on 25 September, 2023, as a case of Cytomegalovirus (CMV ) and Candida albicans associated Conjunctivitis ,after a traumatic sandball inoculation to his right eye . Conjunctiva swab for microscopy and culture was sent to the medical microbiology laboratory. Gram stain showed yeasts cells, while 10%giemsa staining of the eye swab showed ztanck cells. Culture yielded Candida albicans.
 He was placed on chloramphenicol eye drop, steroid eye drops, Acyclovir (patient could not afford gangcyclovir the specific antiviral drug due to high cost) and fluconazole eye drops. Patient was managed by the ophthalmologists and the Medical Microbiology team.
 Patient was discharged home on the 5th day and was asked to come for follow up after one week .On follow up visit , previous symptoms had resolved and right and left eyes were both normal on examination.
 Conclusion: We are unaware of any previous reports of Ophthalmia Adultorium involving Cytomegalovirus and Candida albicans in patients with sandball eye accidents. Patients with sandball injuries should be assessed quickly and antibiotic and antifungal therapy should be considered if cultures are positive.

EVALUATING CHANGES IN SALIVARY FLOW RATE, PH & MICROBIAL COUNT DURING FIXED ORTHODONTIC TREATMENT - AN IN VIVO STUDY

Aim of the study: Aim of this study is to assess the changes in salivary flow rate, salivary pH, plaque index, Streptococcus mutans, Lactobacillus, and Candida albicans in patients undergoing fixed orthodontic treatment, before and after placement of fixed orthodontic appliances. Materials and Method: In present study salivary samples from 60 patients (30 Males and 30 Females) with age group of 14-25 years would be undergoing fixed orthodontic treatment at the department of orthodontics. Salivary flow rate, pH, plaque index and microbial colony count were measured. Streptococcus mutans, Lactobacillus and Candida albicans colony counts were measured using mitis salivarius agar, rogosa agar and sabouraud dextrose agar respectively. Saliva collected for 5 mins and measured in ml/min. salivary pH measured with digital pH meter. Salivary samples obtained from the same patients at intervals of 8 weeks and 16 weeks after commencement of orthodontic treatment and evaluated for the same. Result: This study showed fixed orthodontic appliances increases salivary flow rate (p=0.0001), decreases salivary pH (p=0.03), increases plaque index (p=0.0001), also increases Streptococcus mutans, Lactobacillus and Candida albicans colony count (p=0.0003, p=0.0001and p=0.019 respectively). Independent t test reported salivary flow rate significantly increases more in male patients at 16 weeks interval (p=0.03). Conclusion: The results of this study confirmed that after placement of fixed orthodontic appliances plaque retention increases, which sequentially decrease the salivary pH and decreased salivary pH promote the growth of microbiota.

Antifungal activity of propolis extract against Candida albicans in patients with vulvovaginal candidiasis

Background: The aim was to evaluate the antifungal activity of propolis extract against Candida albicans (or C. albicans) in patients with vulvovaginal candidiasis. Methods: The research presents a quantitative approach with an experimental design, with a population of C. albicans strains isolated from patients diagnosed with vulvovaginal candidiasis who were admitted to the Gynecology Service of the Maria del Socorro Clinic, district of Ate, Lima; a sample of 34 strains was chosen in total, considering three repetitions per strain, obtaining 102 experimental units. The antifungal susceptibility test was carried out by the disc diffusion plate method using a single concentration of propolis extract, with fluconazole as a positive control. Results: Propolis extract was shown to inhibit all strains of C. albicans, as was fluconazole, with the effect of the latter being significantly greater than that of the product under investigation. Conclusions: The conclusion is that propolis extract does show antifungal activity against C. albicans in patients with vulvovaginal candidiasis.

Epidemiological factors associated with Candida albicans in patients using complete denture: a scoping review

Objective: This study undertakes a scoping review of research on epidemiological factors of Candida albicans in patients using complete denture (CD). Methods: PubMed, LILACS, Embase, SciELO, Web of Science, and Scopus databases were used. Searches were conducted in December 2020. Keywords used in this search were Candida albicans, Complete Denture, Mouth, and Microbiology. Results: Initially, 89 articles were identified; 19 of these comprised the final sample after applying the exclusion/inclusion criteria. There was a greater tendency for females to use DC. In addition, among all samples evaluating the incidence of the fungus (n = 2,724), approximately 44.7% (n = 1,218) had C. albicans-associated prosthetic stomatitis. Conclusion: Prosthetic stomatitis is a condition that mainly affects women aged 50–70 years and has a multifactorial predisposition. Also, Newton's classification proposed in 1962 is the most used for the clinical diagnosis of oral candidiasis. The CD's hygiene status is a crucial factor for the progression of candidosis, in the same way that the C. albicans fungus plays an important role in the progression of this pathogenesis in the host.

The First Evidence of a Disturbed Immune Response toward Candida Albicans in Patients with Sars-Cov-2 and Co-Morbility. A Novel Case Report

The First Evidence of a Disturbed Immune Response toward Candida Albicans in Patients with Sars-Cov-2 and Co-Morbility. A Novel Case Report

The First Evidence of a Disturbed Immune Response toward Candida Albicans in Patients with Sars-Cov-2 and Co-Morbility. A Novel Case Report

The First Evidence of a Disturbed Immune Response toward Candida Albicans in Patients with Sars-Cov-2 and Co-Morbility. A Novel Case Report

EPIDEMIOLOGY OF NOSOCOMIAL INFECTION COMPLICATIONS IN A SURGICAL CLINIC

The analysis of the data of literary sources of domestic and foreign researchers on the problem of the occurrence and development of nosocomial infections in general and in the surgical departments in particular is carried out. The relevance of the study is confirmed by the following data: the spread of nosocomial infections in the Russian Federation is 2.5 million annually; the minimum damage inflicted by them in Russia alone is approximately 6-7 million rubles. The data of foreign scientists have been studied, suggesting that infections in the field of hirirurgic intervention were recorded in 13% of cases after performing clean operations, in 16% after and in 29% after . In St. Petersburg, with selective studies, the frequency of infections in the field of surgical intervention revealed 9% of patients among 1,500 operated It was revealed that the number of potential causative agents of nosocomial infections increases every year due to opportunistic bacteria that differ in biological characteristics from hospital strains and have multiple drug antibiotic resistance. A retrospective and prospective analysis of the frequency of occurrence of microorganisms in patients of the surgical department of 2008-2019 was carried out. The microbial spectrum and prevalence of nosocomial infection pathogens in the surgical department were investigated. An epidemiological analysis of the causative agents of nosocomial infection in patients with pneumonia, infections in the surgical field and urinary tract, as well as with generalized infections, was carried out. The analysis of the bacterial flora of the clinical microbiological culture of the wound discharge, sputum, urine and blood was carried out. The following microorganisms were most prevalent in these samples: Staphylococcus aureus, Acinetobacter baumannii, Candida albicans, Klebsiella pneumonia. A tendency to increase the proportion of Candida albicans in patients of the surgical department, as well as a high prevalence of Staphylococcus epidermidis in blood cultures, was revealed.

Effect of celery leaves extract on the growth of Candida albicans in patients wearing removable orthodontic appliances

Background: Maintaining the cleanliness of removable orthodontic appliances is often overlooked so it can lead to changes in Candida albicans into the pathogenic form. Celery leaves are one of the natural ingredients that have active compounds as antimicrobial. Objective: To determine the effects of celery leaf extract in inhibiting the growth of C.albicans found on re-movable orthodontic appliances. Methods: Laboratory experimental studies were conducted with post test only control group design involving 5 samples of removable orthodontic appliances, which were swabbed to obtain C.albicans. Samples were divided into 5 groups, 1 control group and 4 treatment groups with 10%, 30%, 50%, and 70% extract concentration and the inhibitory test was performed. Result: The greater the concentration of celery leaf extract, the greater the diameter of the inhibi-tory zone formed. Conclusion: Celery leaf extract has the effect in inhibiting the growth of C.albicans found on removable or-thodontic appliances.

Effect of binahong leaf extract on the growth of Candida albicans in patients using removable orthodontic appliances

Background: In the oral cavity there are countless microorganisms, about 40% of which are Candida albicans as normal flora. The use of removable orthodontic appliances in the oral cavity greatly inhibits cleanliness and increases the number of retentive areas in the oral cavity resulting in risk factors of increased C.albicans. Binahong leaves contain many active compounds that are antimicrobial. Objective: To determine the effect of binahong leaf extract in inhibiting the growth of C.albicans in patients using removable orthodontic appliances. Method: Laboratory experimental research was conducted with the design of the posttest only control group design involving 5 samples of removable orthodontic appliances which were swabbed to obtain C.albicans, then each tested with binahong leaf extract concentrations of 10%, 30%, 50%, 70% and the control group. Next is the inhibitory test. Results: The greater concentration of the Binahong leaf extract, the greater the diameter of the resistance. Conclusion: Binahong leaf extract has an effect on inhibiting the growth of C.albicans in patients using removable orthodontic appliances.

A pilot study: a possible implication of Candida as an etiologically endogenous pathogen for oral lichen planus

BackgroundThe aim of this study was to investigate the prevalence and genotypic profiles of Candida albicans in patients with oral lichen planus (OLP).Materials and methodsPositive rates and genotypic profiles of Candida albicans strains from OLP patients and healthy controls were analyzed. Random amplified polymorphic DNA and internal transcribed spacer of ribosome DNA polymerase chain reactions were used to sequence the DNA of these strains, and then their genetic similarity was measured using BLAST, UIV Band, and Vector NTI Suite Sequence Analyses Software.ResultsThe prevalence of C. albicans strains detected from erosive-OLP, non-erosive OLP, and normal individuals was 18.87, 18.75, and 7.92%, respectively. Four different genotypes were revealed by the two methods. To be specific, type I was found only in the healthy subjects; type II a and II b were found in non-erosive OLP, and type III was identified in erosive OLP. Intragroup similarity coefficients, i.e. SAB were 100%, and inter-groups similarity coefficients, i.e. SAB were less than 30%.ConclusionsThe genotypic results of C. albicans in OLP revealed an endogenous rather than exogenous infection of C. albicans. In addition, a possible pathogenic role of C. albicans in OLP, with the etiologic sense contributing to a more proper recognition on the pathogenesis, development, and progression of OLP, as well as some strategies for its diagnosis and treatment were identified.

Modulation of the Oral Microbiome to Improve Oral Health and Reduce BSI from Oral Flora in Pediatric Hematopoietic Stem Cell Transplant Recipients: A Randomized Controlled Trial

BackgroundBlood stream infections (BSI) in hematopoietic stem cell transplant (HSCT) patients with central venous catheters may arise from translocation of bacteria through a non-intact gastrointestinal mucosa and are known as mucosal barrier injury–laboratory confirmed blood stream infections (MBI-LCBI). Central venous catheter maintenance care doesn't prevent MBI-LCB and currently there are no proven strategies to prevent bacteremia secondary to mucosal barrier injury. Gingivitis and periodontitis are features of the mucosal toxicity seen post-HSCT and are associated with BSI frequency. Xylitol is a non-fermentable polysaccharide that reduces dental caries, plaque accumulation, and oral disease progression by inhibiting bacterial growth and community development. We hypothesized that daily dental xylitol, in addition to current oral care practice, is effective at reducing gingivitis, periodontitis and BSI from oral organisms.MethodsWe performed a prospective randomized controlled trial to test our hypothesis. All patients received standard oral care and half were randomized to additionally receive daily xylitol. Oral exams were performed at baseline and at intervals for the first 28 days post-HSCT. Metagenomic shotgun sequencing of gingival samples was performed at these same intervals. For patients who develop BSI, whole genome sequencing of bacterial isolates is being performed to assess for genetic relatedness to corresponding strains present within the patient's oral microbiome preceding the infection.ResultsWe enrolled 35 patients, 17 cases and 18 controls before ending the study early for efficacy. Five patients withdrew from the study (3 cases, 2 controls). Patients who received xylitol had a significantly lower rate of gingivitis, oral plaque and oral ulcers greater than 10 mm. There was a significant decrease in BSI from oral organisms in the first 30 days post HSCT in the xylitol group, 0/14 (0%) vs 5/16 (31%) (p = 0.04). Preliminary microbiome analysis showed decrease relative abundance of Candida albicans in patients receiving xylitol and individual patient analysis shows that xylitol may also impede Streptococcus mitis/oralis dominance in the oral microbiome. Additionally, we found that Staphylococcus aureus, a common cause of Central Line Associated Blood Stream Infection (CLABSI), was prominent in several gingival samples, suggesting BSI with Staphylococcus aureus may indeed be related to mucosal barrier injury.ConclusionsThe addition of xylitol to oral standard care appears to decrease BSI secondary to oral organisms, dental plaque, gingivitis and oral ulcerations in patients undergoing SCT. Blood stream infections (BSI) in hematopoietic stem cell transplant (HSCT) patients with central venous catheters may arise from translocation of bacteria through a non-intact gastrointestinal mucosa and are known as mucosal barrier injury–laboratory confirmed blood stream infections (MBI-LCBI). Central venous catheter maintenance care doesn't prevent MBI-LCB and currently there are no proven strategies to prevent bacteremia secondary to mucosal barrier injury. Gingivitis and periodontitis are features of the mucosal toxicity seen post-HSCT and are associated with BSI frequency. Xylitol is a non-fermentable polysaccharide that reduces dental caries, plaque accumulation, and oral disease progression by inhibiting bacterial growth and community development. We hypothesized that daily dental xylitol, in addition to current oral care practice, is effective at reducing gingivitis, periodontitis and BSI from oral organisms. We performed a prospective randomized controlled trial to test our hypothesis. All patients received standard oral care and half were randomized to additionally receive daily xylitol. Oral exams were performed at baseline and at intervals for the first 28 days post-HSCT. Metagenomic shotgun sequencing of gingival samples was performed at these same intervals. For patients who develop BSI, whole genome sequencing of bacterial isolates is being performed to assess for genetic relatedness to corresponding strains present within the patient's oral microbiome preceding the infection. We enrolled 35 patients, 17 cases and 18 controls before ending the study early for efficacy. Five patients withdrew from the study (3 cases, 2 controls). Patients who received xylitol had a significantly lower rate of gingivitis, oral plaque and oral ulcers greater than 10 mm. There was a significant decrease in BSI from oral organisms in the first 30 days post HSCT in the xylitol group, 0/14 (0%) vs 5/16 (31%) (p = 0.04). Preliminary microbiome analysis showed decrease relative abundance of Candida albicans in patients receiving xylitol and individual patient analysis shows that xylitol may also impede Streptococcus mitis/oralis dominance in the oral microbiome. Additionally, we found that Staphylococcus aureus, a common cause of Central Line Associated Blood Stream Infection (CLABSI), was prominent in several gingival samples, suggesting BSI with Staphylococcus aureus may indeed be related to mucosal barrier injury. The addition of xylitol to oral standard care appears to decrease BSI secondary to oral organisms, dental plaque, gingivitis and oral ulcerations in patients undergoing SCT.

A Case Report of a Successful Allergen Immunotherapy with Candida Albicans in Patient with Sever Atopic Dermatitis Sensitive to Candida Albicans

Introduction: Atopic dermatitis (AD) is an inflammatory, chronic, relapsing, itchy skin disease with an immunologic basis. This disease is associated with itchy skin lesions (pruritus), dry skin (xerosis) and plaques of eczema. The role of aeroallergens, such as house dust mite (HDM) and food allergens has been proven to exacerbate skin eczema lesions. Alongside drugs such as corticosteroids, topical emollients, antihistamines, tacrolimus, and immune suppressants, phototherapy and subcutaneous immunotherapy (SCIT) also done. SCIT is mostly using for sensitization to mite allergens.Case Presentation: We present a 30 y/o Iranian woman with severe atopic dermatitis and sensitization to Candida allergens. We initiated SCIT with candida allergen and the patient had obvious improvements in her signs and symptoms 3 months after starting SCIT.Conclusion: Although subcutaneous immunotherapy was only approved for mite sensitization in atopic dermatitis, it should be considered in other aeroallergen sensitizations.

Autoimmunity and intestinal colonization by Candida albicans in patients with type 1 diabetes at the time of the diagnosis

Purpose Type 1 diabetes mellitus (T1DM) is a chronic and immune-mediated disease, which is characterized by the progressive destruction of pancreatic beta cells. T1DM precipitates in genetically susceptible individuals through environmental factors. In this study, we aimed to evaluate the impact of autoimmunity and intestinal colonization of Candida albicans on the development of T1DM.Methods Forty-two patients newly diagnosed with T1DM and 42 healthy subjects were included in this monocentric study. The basic and clinical characteristics of the patients were recorded. T1DM-, thyroid-, and celiac-associated antibodies were evaluated. Stool cultures for C. albicans were performed to assess whether or not gut integrity was impaired in patients with T1DM.Results The evaluation of T1DM- and thyroid-associated antibodies showed that the prevalences of islet cell antibodies and antithyroperoxidase positivity were higher in the study patients than in the patients in the control group. Furthermore, the direct examination and culture of fresh stool samples revealed that 50% of the patients with T1DM and 23.8% of the control subjects had fungi (C. albicans).Conclusion Through this study, we suggest that the presence of intestinal C. albicans colonization at the time of the diagnosis of T1DM may indicate impairment of normal intestinal microbiota. We also suggest that there may be a tendency of T1DM in patients with a high prevalence of intestinal C. albicans.

PREVALENCIA DE CANDIDA ALBICANS EN INDIVIDUOS PORTADORES DE PRÓTESIS TOTAL QUE ASISTEN A LA CLÍNICA ODONTOLÓGICA DE LA UNIVERSIDAD SANTO TOMÁS Y A INSTITUCIONES GERIÁTRICAS DE FLORIDABLANCA

Objective: To identify the frequency of Candida albicans in patients with dental prosthesis with or without signs and evident clinical symptoms those attend the Dental Clinics of the Santo Tomas University and geriatric institutions of Floridablanca in the period of a year.Materials and methods: An analytical observational study of cross section was made. The collected information related to clinical background in oral cavity and general demographics variables. The prevalence of Candida albicans was determined by means of the test of germinal, blue tube of lactofenol and hydroxide of potassium, to statistical analysis like chi2 and exact test of Fisher were applied.Results: Seventy samples, 47 of them in patients with signs and symptoms of candidiasis were taken (67.1%), 38 located in palate, being eritema of palate most frequent in a 37.1%. The laboratory tests of blue of lactofenol (ALF) and hydroxide of potassium (KOH) turned out to be specific but not it sufficiently sensible. On the other hand, the germinal tube test demonstrated to be positive in 47 samples with a prevalence of (67.14%) of the patients in samples of oral mucosa and 53 (75.71%) in prosthesis samples.Conclusions: It was an associate important prevalence with the use of total prosthesis and the presence of signs and symptoms, specially, red injuries of the palatal mucosa that suggests an important relation with the diagnosis of prosthesis stomatitis.

Candida albicans in patients with denture stomatitis of the center for the elderly adult, Chiclayo, Perú

Objective: Determine if there is an association between the presence of Candida albicans and the denture stomatitis type in patients who attend to the aged center of Chiclayo – July 2012. Materials and Methods: 58 patients with removable dental prostheses diagnosed clinically with denture stomatitis were evaluated. A palatal mucosal swab was performed and then cultured directly in the medium of the CHROMagar Candida culture at 37 ° C for 48 hours. The crop that tested positive for Candida albicans were submitted to germ-tube testing. Results: A total of 35 (60%) cultures tested positive for Candida albicans, with grade III of denture stomatitis being 100% associated with Candida albicans (n = 3), grade II presented 70.8% (n=17). The total prosthesis was more involved in the presence of Candida albicans (65.5%) with no statistical significance, p= 0.591. Conclusions: There is association between the denture stomatitis and Candida albicans. Key words: Candida albicans; denture st

Evidence of an abnormal epithelial barrier in active, untreated and corticosteroid-treated eosinophilic esophagitis.

Eosinophilic esophagitis (EoE) is a chronic, immune/antigen-mediated disease characterized by symptoms related to esophageal dysfunction and an eosinophil-predominant inflammation. This study has aimed to investigate whether the recently observed sensitization to Candida albicans in patients with EoE is owing to pre-existing disease and its underlying abnormal epithelial barrier or, alternatively, is linked to corticosteroid (CS) therapy. Medical histories, as well as serum and tissue samples of 60 patients with EoE (15 CS naive, 45 with current or previous CS therapy) and 20 controls, stored in the Swiss Eosinophilic Esophagitis Database (SEED) and Biobank, were analyzed. We applied ImmunoCAP to measure IgE levels and immunofluorescence techniques to examine epithelial barrier components. Patients with EoE had higher total IgE levels and were more frequently sensitized to C. albicans than controls. In EoE tissue specimens, increased numbers of eosinophils and mast cells, a higher expression levels of thymic stromal lymphopoietin (TSLP), cathelicidin, proteases, that is, the kallikreins (KLK)-5 and KLK-7, were observed as compared with controls, while reduced expression of lympho-epithelial Kazal-type-related inhibitor (LEKTI), filaggrin, E-cadherin, claudin, occludin, desmoglein-1 was found, independent of CS therapy. In CS-treated EoE, significantly lower numbers of CD1a+ cells and cathelicidin expression were noted as compared to CS-naive EoE. This study provides further evidence that EoE is associated with an abnormal epithelial barrier and postulates that CS therapy, by reducing innate immune mechanisms, may promote C. albicans colonization and likely subsequent sensitization.

Oral Candida albicans in Patients in the ICU of a Brazilian Hospital School and in vitro Susceptibility of Isolated Yeasts to Extra-virgin Coconut Oil

Oral Candida albicans in Patients in the ICU of a Brazilian Hospital School and in vitro Susceptibility of Isolated Yeasts to Extra-virgin Coconut Oil

The Effect of Gold Nano Particles Compared to Dioxide Titanium Nano Particles on Vital Factors of Isolated Candida albicans in Patients with Oral Candidiasis in Vitro

Background: Oral Candidiasis is fungal infection that affects the oral cavity. Oral infections caused by yeast of the genus Candida and particularly Candida albicans (oral candidiasis) have been recognized throughout recorded history. Objectives: The aim of this study was to compare the antifungal effects of gold nanoparticles and dioxide titanium nanoparticles on patients with Oral Candidiasis patients. This review is to give the reader a contemporary overview of oral candidiasis, the organisms involved, and the management strategies that are currently employed or could be utilized in the future. Methods: This experimental study has been done in Isfahan city totally with 56 numbers of patients suffering from Candidiasis in groups of different ages from hospitals and laboratories The resulted from swap in Sabouraud Dexteros agar and finally with complementary experiments 56 isolated Candida albicans (oral Candidiasis) detected and grew in culture milieu then gold nanoparticles (10 nanometers) and titanium dioxide nanoparticles (10 - 15 nanometers) in different consistencies add to this milieu and the least density of halting and the least density of killing fungi for different suspension thinness containing Candida albicans. The data were analyzed by spss 15 version software. Results: The results showed that gold nanoparticles have a good anticandidial effects and can be used to treat infections of Candida, it is recommended that further research considered the effects of different infections candidiasis in In vitro condition. Conclusions: Using gold nanoparticles with 10 nanometer diameters have high antifungal effect on oral candidiasis and its function has been proved. In current study halting effect of gold nanoparticles on micro-organisms experimented in different densities was observed.