The development of diseases of the pelvic organs microbial etiology depends on the properties of persistent microorganisms aimed at inactivating factors natural resistance of the organism. The species composition and biological properties of the microflora of the vagina and cervical canal in women with inflammatory diseases of internal genital organs, and shown that they occur against the background dysbiotic states. In patients with chronic salpingoophoritis vaginal microflora changes occur, accompanied by incoordination its functioning as a single ecosystem, which is manifested disorders microbiological status due to the formation of biofilms, they in turn, is a major survival strategies of bacteria in the environment, which is realized only if achievement a certain level of population density. The purpose of the study is to improve the diagnosis and treatment of patients with chronic salpingo (HSO) based on determination mikrobiotsynozu vagina.The study evaluated the clinical status and characteristics of vaginal microflora in 70 patients with HSO depending on the limitations of the inflammatory process. The study patients breach vaginal microbiota. Analyzing the data obtained, it was found that the most common microorganisms that removed from the vaginal discharge were: Peptostreptococcus spp - 78% and 75%, Enterococcus - 69% and 57%, S.aureus - 62,9% and 60, 1%, E.coli - 64,2% and 69,3%, Fusobacterium spp - 61,0% and 58.0%; S. pyogenes - 58,0% and 60%, Candida spp - 47,0% and 44%.The results of testing the ability of isolates to form biofilms showed that all pathogens are removed form a dense biofilm. Analysis of the results showed that planktonic cells pathogens tested, actively formed biofilm within 24 hours, and anaerobic bacteria - within 48 hours; the degree bioplivkoutvorennya most active were staphylococci, E. coli, and fungi of the genus Candida, actinomycetes. Results of the study can establish the facts of the formation of biofilms isolates microorganisms asotsiantiv depending on the facts and confirm that this reflects the adaptive capacity pathogen. Investigation of the sensitivity of microorganisms in the form of biofilms to antimicrobials found that they adverse drug at therapeutic doses.Thus dysbiotic abuse play a significant role in the pathogenesis salpingo flow and deepen the severity of complications and only purpose of adjuvant therapy with the inclusion of immunomodulating drugs can normalize and promote vaginal mikrobiotsynoz reduced chronic inflammation. This creates prerequisites for further research on sustainable correction therapies and a complete description of the mechanisms that contribute to the development of inflammatory diseases and research background biofilms.