We conducted two experiments to evaluate the impact of cultural treatments on growth and establishment of container-grown London planetrees (Platanus × acerifolia’ Bloodgood’). In both experiments, 48 trees grown in 100 l (#25) black plastic containers were assigned at random to one of three root-ball treatments prior to planting; no treatment (Control), outer 3 cm of roots removed around entire root-ball (Shave), or outer circling roots disentangled from the root-ball (Tease). In Experiment 1, half of the trees were fertilized with 400 g of controlled release fertilizer (15-9-12; N-P2O5-K2O) at planting and the remainder of the trees were not fertilized. In Experiment 2, half of the trees were mulched with an 8 cm deep × 2 m diameter ring of coarse ground pine bark at planting and the remainder of the trees were not mulched. In Experiment 1, fertilization at planting increased SPAD chlorophyll content on two of four measurement dates but did not affect cumulative height or caliper growth after two years. After two growing seasons, root-ball treatments (shaving or teasing) increased root growth outside the original root-ball compared to control trees. Both root-ball treatments also reduced circling roots. In Experiment 2, mulching at planting increased soil moisture and cumulative tree height and diameter growth. Shaving increased new root growth and both root-ball treatments improved root architecture and reduced circling roots. Overall, the study demonstrates that root-ball manipulations can stimulate new root growth and reduce circling roots. Mulch is a valuable aid to conserve soil moisture and increase tree growth. Fertilization at planting provided little benefit in this experiment, which may have been related to a high level of soil fertility at the site or nutrient loading of the trees from nursery culture prior to transplanting.