Calcium Phosphate Silicate Research Articles

Calcium-based triphasic powder synthesis for strengthening 3D printed bone scaffolds

This study aims to improve the mechanical properties of biocompatible ceramic materials, which are known for their superior potential to integrate with bone compared to metallic implants. However, achieving sufficient mechanical strength, particularly for weight-bearing areas, poses a challenge. Hydroxyapatite (HA) powder is synthesized, taking into consideration the mechanical mixing and aging effects to achieve the desired composition and properties. The synthesized HA powder undergoes calcination under various atmospheres to assess its impact on the crystal structure. The study also explores the coating of HA particles with silicon dioxide (SiO2) using tetraethyl orthosilicate (TEOS) to enhance surface modification and wettability. This process results in a triphasic powder consisting of HA, tricalcium phosphate (TCP), and calcium phosphate silicate (CPS). The morphology of the powder is analyzed using techniques such as energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), and X-ray diffraction (XRD). Moreover, a refined sintering procedure is developed to minimize cracking volume, leading to a significant 117 % increase in compressive strength compared to commercial materials. Finally, the powder is optimized for 3D printing by adding water, simplifying its use in biomedical applications.

Biomimetic Scaffolds of Calcium-Based Materials for Bone Regeneration.

Calcium-based materials, such as calcium carbonate, calcium phosphate, and calcium silicate, have attracted significant attention in biomedical research, owing to their unique physicochemical properties and versatile applications. The distinctive characteristics of these materials, including their inherent biocompatibility and tunable structures, hold significant promise for applications in bone regeneration and tissue engineering. This review explores the biomedical applications of calcium-containing materials, particularly for bone regeneration. Their remarkable biocompatibility, tunable nanostructures, and multifaceted functionalities make them pivotal for advancing regenerative medicine, drug delivery system, and biomimetic scaffold applications. The evolving landscape of biomedical research continues to uncover new possibilities, positioning calcium-based materials as key contributors to the next generation of innovative biomaterial scaffolds.

A ternary calcium silicate bone cement with high mechanical properties and good operability

The treatment of vertebral compression fractures of the spinal necessitates the use of injectable bone repair materials with the adaptive modulus of elasticity. Currently, newly developed injectable self-curing materials for minimally invasive surgery are primarily categorized into calcium phosphate and calcium silicate systems, both of which exhibit excellent biocompatibility. However, calcium phosphate bone cement lacks adequate mechanical properties and the stress does not match between bone and material. Calcium silicate cement has a long curing time, low mechanical strength, and high alkalinity. These deficiencies render them inadequate for meeting clinical requirements. Therefore, we incorporated inorganic disodium phosphate, organic polyvinyl alcohol, and solid component cerium oxide into the matrix phase of tricalcium silicate bone cement to establish a ternary self-curing hydration reinforcement system and produced an organic-inorganic composite injectable bone cement. Even though the composite bone cement has a longer setting time, it exhibited high compressive strength (61.9Mpa) and fracture strain (7.88 %), demonstrating excellent mechanical properties. Furthermore, it also demonstrates good injectable properties (86 %), anti-collapse properties, good biological activity, and outstanding antibacterial properties. The composite bone cement has great potential to develop a new injectable self-curing material to replace PMMA bone cement in clinical vertebral compression fractures.

Evaluation of the elemental composition and radiological density of bone tissue when replacing a metaphyseal defect with bioceramic phosphate-silicate granules (experimental study)

Background: It is known that bioceramic implants containing various calcium or silicon compounds in isolation demonstrate osteoconductive effect in the replacement of post-traumatic bone defects. The combined use of these elements in single material should potentiate the organotypic filling of the bone cavity by creating favorable ion microenvironment and staged biodegradation. AIM: To identify the correlation of radiological indicators of the density of newly formed bone tissue and content of micro- and macronutrients in a bone defect when it is replaced by bioceramics with various mass ratio of calcium phosphate and silicate. MATERIALS AND METHODS: The study was performed on male rabbits of the “white giant” breed, which, after receiving a standardized delimited metaphysical bone defect, implants with variable ratio of calcium phosphate and calcium silicate (in proportions of 40/60, 50/50 and 60/40 wt. %) were used to replace it. The results were evaluated using multispiral computed tomography and scanning electron microscopy energy dispersive analysis with detection by the method of correlation analysis of possible connections between the obtained data. RESULTS: Quantitative indicators of calcium and phosphorus content in bone regenerate in all groups increased mainly in the period from 30 to 60 days, and silicon content, reaching maximum amounts by the 30th day of the experiment, subsequently decreased monotonously, which showed participation of this element in the starting regenerative processes, and its decrease served as a marker of organotypic restructuring. In the elemental analysis of newly formed bone tissue during implantation of bioceramics containing phosphate and calcium silicate in the proportion of 60/40 wt. %. The highest amounts of calcium, phosphorus and silicon and the highest density of newly formed bone tissue were noted, which had direct correlation, and this pattern was observed both in the early stages (30 days) and throughout the experimental study. CONCLUSION: Analyzing the data obtained, it can be concluded that it is advisable to study the features of the course of reparative osteogenesis depending on the ionic environment, as well as the high potential of using synthetic bioceramics in general and the prospects of using implants on the basis of phosphate-silicate composites for bone defects replacement.

Evaluation of baghdadite (Ca3ZrSi2O9) cements for the application as novel endodontic filling materials

ObjectivesBaghdadite (Ca3ZrSi2O9) cements of various composition have been investigated in this study regarding an application as endodontic filling materials. MethodsCements were either obtained by mixing mechanically activated baghdadite powder with water (maBag) or by subsequently substituting the ß-tricalcium phosphate (ß-TCP) component in a brushite forming calcium phosphate cement. The cements were analyzed for their mechanical performance, injectability, radiopacity, phase composition and antimicrobial properties. ResultsThe cements demonstrated sufficient mechanical performance with a compressive strength of ∼1 MPa (maBag) and 2.3 – 17.4 MPa (substituted calcium phosphate cement), good injectability > 80 % depending on the powder to liquid ratio and an intrinsic radiopacity of 1.13 – 2.05 mm aluminum equivalent. Immersion in artificial saliva proved their bioactivity by the formation of calcium phosphate and calcium silicate precipitates on the cement surface. The bacterial activity of Staphylococcus aureus cultured on the surface of the cements was found to be similar compared to clinical standard ProRoot MTA cement or even reduced by a factor of 3 for Streptococcus mutans. SignificanceIn combination with their antibacterial properties, baghdadite cements are thought to have the potential to fulfil the clinical requirements for endodontic filling materials.

Limitations and modifications in the clinical application of calcium sulfate.

Calcium sulfate and calcium sulfate-based biomaterials have been widely used in non-load-bearing bone defects for hundreds of years due to their superior biocompatibility, biodegradability, and non-toxicity. However, lower compressive strength and rapid degradation rate are the main limitations in clinical applications. Excessive absorption causes a sharp increase in sulfate ion and calcium ion concentrations around the bone defect site, resulting in delayed wound healing and hypercalcemia. In addition, the space between calcium sulfate and the host bone, resulting from excessively rapid absorption, has adverse effects on bone healing or fusion techniques. This issue has been recognized and addressed. The lack of sufficient mechanical strength makes it challenging to use calcium sulfate and calcium sulfate-based biomaterials in load-bearing areas. To overcome these defects, the introduction of various inorganic additives, such as calcium carbonate, calcium phosphate, and calcium silicate, into calcium sulfate is an effective measure. Inorganic materials with different physical and chemical properties can greatly improve the properties of calcium sulfate composites. For example, the hydrolysis products of calcium carbonate are alkaline substances that can buffer the acidic environment caused by the degradation of calcium sulfate; calcium phosphate has poor degradation, which can effectively avoid the excessive absorption of calcium sulfate; and calcium silicate can promote the compressive strength and stimulate new bone formation. The purpose of this review is to review the poor properties of calcium sulfate and its complications in clinical application and to explore the effect of various inorganic additives on the physicochemical properties and biological properties of calcium sulfate.

Fibroin reinforced, strontium-doped calcium phosphate silicate cements for bone tissue engineering applications

Fibroin reinforced, strontium-doped calcium phosphate silicate cements for bone tissue engineering applications

Identification of defect centers responsible for thermoluminescence emission in sol-gel synthesized calcium silicate phosphor

The thermoluminescence (TL) of calcium silicate phosphor (CSO) prepared by the sol-gel method and sintered at 1200 °C were investigated. From Tm-Tstop curve, TL emission spectrum and computer deconvolution using electron traps with discrete and continuous distributions, the glow curves were found to be composed of four TL peaks (117, 190, 250 and 275 °C) with a single emission band centered at 370 nm. Electron paramagnetic resonance (EPR) investigation has been carried out to identify the defect centers formed in the CSO phosphor by γ-irradiation and find the centers related to the TL process in the phosphor. At room temperature, three defect centers were observed. The first center, characterized by the principal g-values of 2.014, 2.011, and 2.0080 was assigned to an O− ion. The second center with g-values 2.015, 2.013, and 2.010 is also attributed to an O− ion and is associated with the TL peak at 280 °C. The third center, with an isotropic g-value of 2.0011 was identified as the F+ center (singly ionized oxygen vacancy) and relates to the TL peak at 280 °C.

3D interconnected porous PMMA scaffold integrating with advanced nanostructured CaP-based biomaterials for rapid bone repair and regeneration

Bioactive scaffolds with polymer and nanostructured bioactive glass-based composites are promising materials for regenerative applications in consequence of close mimics of natural bone composition. Poly methyl methacrylate (PMMA) is a highly preferred thermoplastic polymer for orthopedic applications as it has good biocompatibility. Different kinds of bioactive, biodegradable as well as biocompatible biomaterial composites such as Bioglass (BG), Hydroxyapatite (Hap), and Tricalcium phosphate (TCP) can be integrated with PMMA, so as to augment the bioactivity, porosity as well as regeneration of hard tissues in human body. Among the bioactive glass, 60S BG (Bioactive glass with 60 percentage of Silica without Sodium ions) is better materials among aforementioned systems owning to mechanical stability as well as controlled bioactive material. In this work, the fabrication of PMMA-CaP (calcium phosphate)-based scaffolds were carried out by Thermal Induced Phase Separation method (TIPS). X-ray diffractogram analysis (XRD) is used to examine the physiochemical properties of the scaffolds that evidently reveal the presence of calcium phosphate besides calcium phosphate silicate phases. The Field Emission Scanning Electron Microscopy (FESEM) studies obviously exhibited the microstructure of the scaffolds as well as their interconnected porous morphology. The PMMA/60S BG/TCP (C50) scaffold has the maximum pore size, measuring 77 ± 23 μm, while the average pore size ranges from 50 ± 20 to 80 ± 23 μm. By performing a liquid displacement method, the C50 scaffold is found to have the largest porosity of 50%, high hydrophilicity of 118.16°, and a compression test reveals the scaffolds to have a maximum compressive strength of 0.16 MPa. The emergence of bone-like apatite on the scaffold surface after 1st and 21st days of SBF immersion is further supported by in vitro bioactivity studies. Cytocompatibility and hemocompatibility analyses undoubtedly confirmed the biocompatibility behavior of PMMA-based bioactive scaffolds. Nano-CT investigation demonstrates that PMMA-CaP scaffolds provide more or less alike morphologies of composites that resemble the natural bone. Therefore, this combination of scaffolds could be considered as potential biomaterials for bone regeneration application. This detailed study promisingly demonstrates the eminence of the unique scaffolds in the direction of regenerative medicines.

Structural, optical, and luminescence properties of Dy3+ -activated potassium calcium silicate phosphor for white light-emitting diodes.

A dysprosium (Dy3+ )-activated potassium calcium silicate (K4 CaSi3 O9 ) phosphor was prepared using a solid-state synthesis route. The phosphor had a cubic structure with the space group Pa as confirmed using X-ray diffraction (XRD) measurements. Details of surface morphology and elemental composition of the as-synthesized undoped KCS phosphor was obtained using scanning electron microscopy (SEM) and energy-dispersive X-ray (EDX) spectroscopy. The chemical structure as well as the vibrational modes present in the as-prepared KCS phosphor was analyzed using Fourier transform infrared (FT-IR)spectroscopy. Diffuse reflectance spectra (DRS) were used to determine the optical bandgap of the phosphors and were found to be in the optical range 3.52-3.71 eV. Photoluminescence (PL) spectra showed intense yellow emission corresponding to the 4 F9/2 →6 H13/2 transition under 350 nm excitation. Commission International de l'Eclairage colour chromaticity coordinates were evaluated using the PL spectral data lie within the white region. Dexter theory and the Inokuti-Hirayama (I-H) model were applied to study the nature of the energy transfer mechanism in the as-prepared phosphors. The relatively high activation energy of the phosphors was evaluated using temperature-dependent PL (TDPL) data and confirmed the high thermal stability of the titled phosphor. The abovementioned results indicated that the as-prepared KCS:Dy3+ phosphor was a promising candidate for n-UV-based white light-emitting diodes.

Study of mineralization of lithium calcium phosphosilicate glass ceramics in vivo during bone tissue regeneration

Prospective directions for the creation of biologically active substitutes for bone tissue were analyzed. The effectiveness of the use of calcium phosphosilicate materials modified with CuO, ZnO, Ag2O, Fe2O3, TiO2, SrO and Nb2O5 to ensure high biocompatibility and antibacterial properties of bone endoprostheses has been established. The prospective use of lithium calcium phosphate silicate glass ceramics for obtaining strengthened, biologically active bone implants was substantiated. The main criteria for the development of biocompatible glass-ceramic materials regarding their composition, structure, texture, and surface properties have been established. The influence of differences in the structure and resorption of calcium phosphosilicate glass ceramics on the mechanism of formation of an apatite-like layer in vivo was analyzed. The features of mineralization of calcium phosphosilicate glass ceramics in vivo during bone tissue regeneration were determined, and the effectiveness of the use of glass ceramics based on hydroxyapatite and lithium disilicate in bone tissue replacement was established to reduce the rehabilitation period and long-term use of endoprostheses under variable loads. The developed OS-7 calcium phosphosilicate glass-ceramic material is characterized by the content of crystalline phases of 10 vol.% lithium disilicate and 55 vol.% hydroxyapatite with a ratio of CaO/P2O5=1.67, surface microrelief of 6 m, surface free energy value of 75 mJ/m2 and crack resistance of 6.0 MPam1/2. This material is biocompatible due to the formation of carbonate hydroxyapatite crystals already on the 14th day, which allows us to consider its promising use in the treatment of fractures, defects of long bones and in the replacement of short or tubular bones.

Europium-Doped Calcium Silicate Nanoparticles as High-Quantum-Yield Red-Emitting Phosphors.

Europium ion-activated calcium silicate phosphors (Ca2SiO4:Eu3+) with sharp red-light emission were fabricated via the hydrothermal method. The size of Ca2SiO4:Eu3+ phosphors was controlled between 20 and 200 nm by precursor silicate particle sizes. Systematic studies to determine morphology, crystal phase, and photoluminescence (PL) were carried out for all the phosphors, and their optical efficiencies were compared. We found that the luminescence intensity and emission wavelength of Ca2SiO4:Eu3+ phosphors depend on their particle sizes. Particularly, the Ca2SiO4:Eu3+ synthesized with 20 nm silica seed contains the most intense red emission, high color purity, and high PL quantum yield. For the 20 nm-sized Ca2SiO4:Eu3+ phosphor, PL quantum yields are measured to be above 87.95% and high color purity of 99.8%. The unusually high intensity of 5D0 → 7F4 emission (712 nm) is explained by structural distortion arising from silicate particle size reductions. We show that the obtained phosphor is a suitable candidate for solid-state lighting as a red component through CIE chromaticity coordinate and color purity measurements. Furthermore, the Ca2SiO4:Eu3+ particles are examined for their validity as promising bio-imaging probes through cell labeling and imaging experiments and biodegradability studies.

Calcium Phosphate Silicate Microspheres with Soybean Lecithin as a Sustained-Release Bone Morphogenetic Protein-Delivery System for Bone Tissue Regeneration.

Bone morphogenetic protein (BMP) is a growth factor that effectively promotes osteogenesis. Microsphere-based drug-delivery systems can facilitate an increase in the local concentration of BMP, thus promoting bone formation. In this study, calcium phosphate silicate (CPS) microspheres were used as drug-loading systems for BMP. Three groups─CPS, CPS + BMP, and CPS + BMP + soy lecithin (SL)─were set up, where SL was used to prolong the osteogenic effect of the microsphere system. Bone marrow mesenchymal stem cells and femoral defects in rats were used to compare the osteogenic ability of the three groups. The results indicated that CPS microspheres were good carriers of BMP, facilitating a smoother release into the cells and tissues. SL loading improved the loading rate of BMP, which promoted the osteogenic effect of the microspheres with BMP. We propose CPS microspheres as potential drug-delivery systems that can be effectively used in the treatment of bone defects.

The effects of BaTiO3 on the handleability and mechanical strength of the prepared piezoelectric calcium phosphate silicate for bone tissue engineering

Natural bone is a piezoelectric material that can generate electrical signals when subjected to an external force. Although many studies have attempted to develop piezoelectric biomaterials for bone regeneration, post-treatment steps, such as sintering, are always needed. In this study, we prepared an injectable and piezoelectric bone substitute based on nanosized BaTiO3 (nBT)-added calcium phosphate silicate (CPS). The impacts of nBT on the CPS handleability and mechanical strength were characterized, and show that adding nBT could improve the CPS handleability but affect the CPS mechanical strength in a concentration-dependent manner (from 25.3 ± 1.0 MPa for 10BC to 13.5 ± 1.0 MPa for 40BC). In addition, our approach could fabricate a piezoelectric bone substitute with comparable piezoelectricity to the native bone without any post-treatment. The in vitro analyses demonstrated that nBT/CPS was biocompatible and could promote osteoblast differentiation. In conclusion, our results strongly indicate that the injectable formulation based on nBT/CPS can be a promising candidate in bone tissue engineering, and further research is needed to investigate the biomaterial's performance in bone defect animal models.

Alkaline-Activation Technique to Produce Low-Temperature Sintering Activated-HAp Ceramic

The fabrication of hydroxyapatite (HAp) ceramics prepared by existing conventional sintering requires high-temperature sintering of 1250 °C to 1300 °C. In this paper, the activated metakaolin (MK)/HAp specimens were prepared from varied mix design inputs, which were varied solid mixtures (different amounts of MK loading in HAp) and liquid-to-solid (L/S) ratios, before being pressed and sintered at 900 °C. Phase analysis, thermal analysis, surface morphology, and tensile strength of the specimens were investigated to study the influences of the Al, Si, Fe, Na, and K composition on the formation of the hydroxyapatite phase and its tensile strength. XRD analysis results show the formation of different phases was obtained from the different mix design inputs HAp (hexagonal and monoclinic), calcium phosphate, sodium calcium phosphate silicate and calcium hydrogen phosphate hydrate. Interestingly, the specimen with the addition of 30 g MK prepared at a 1.25 L/S ratio showed the formation of a monoclinic hydroxyapatite phase, resulting in the highest diametrical tensile strength of 12.52 MPa. Moreover, the increment in the MK amount in the specimens promotes better densification when sintered at 900 °C, which was highlighted in the microstructure study. This may be attributed to the Fe2O3, Na2O, and K2O contents in the MK and alkaline activator, which acted as a self-fluxing agent and contributed to the lower sintering temperature. Therefore, the research revealed that the addition of MK in the activated-HAp system could achieve a stable hydroxyapatite phase and better tensile strength at a low sintering temperature.

Powder Synthesized from Aqueous Solution of Calcium Nitrate and Mixed-Anionic Solution of Orthophosphate and Silicate Anions for Bioceramics Production

Synthesis from mixed-anionic aqueous solutions is a novel approach to obtain active powders for bioceramics production in the CaO-SiO2-P2O5-Na2O system. In this work, powders were prepared using precipitation from aqueous solutions of the following precursors: Ca(NO3)2 and Na2HPO4 (CaP); Ca(NO3)2 and Na2SiO3 (CaSi); and Ca(NO3)2, Na2HPO4 and Na2SiO3 (CaPSi). Phase composition of the CaP powder included brushite CaHPO4‧2H2O and the CaSi powder included calcium silicate hydrate. Phase composition of the CaPSi powder consisted of the amorphous phase (presumably containing hydrated quasi-amorphous calcium phosphate and calcium silicate phase). All synthesized powders contained NaNO3 as a by-product. The total weight loss after heating up to 1000 °C for the CaP sample—28.3%, for the CaSi sample—38.8% and for the CaPSi sample was 29%. Phase composition of the ceramic samples after the heat treatment at 1000 °C based on the CaP powder contained β-NaCaPO4 and β-Ca2P2O7, the ceramic samples based on the CaSi powder contained α-CaSiO3 and Na2Ca2Si2O7, while the ceramics obtained from the CaPSi powder contained sodium rhenanite β-NaCaPO4, wollastonite α-CaSiO3 and Na3Ca6(PO4)5. The densest ceramic sample was obtained in CaO-SiO2-P2O5-Na2O system at 900 °C from the CaP powder (ρ = 2.53 g/cm3), while the other samples had densities of 0.93 g/cm3 (CaSi) and 1.22 (CaPSi) at the same temperature. The ceramics prepared in this system contain biocompatible and bioresorbable phases, and can be recommended for use in medicine for bone-defect treatment.

Different cellulose nanofibers impact properties of calcium phosphate silicate cement for bone tissue engineering

Different cellulose nanofibers impact properties of calcium phosphate silicate cement for bone tissue engineering

A Preliminary Review of Modified Polymethyl Methacrylate and Calcium-Based Bone Cement for Improving Properties in Osteoporotic Vertebral Compression Fractures

The incidence of osteoporotic vertebral compression fractures (OVCFs) increases gradually with age, resulting in different degrees of pain for patients, even possible neurological damage and deformity, which can seriously affect their quality of life. Vertebral augmentation plays an important role in the surgical treatment of OVCFs. As the most widely used bone cement material, polymethyl methacrylate (PMMA) offers inherent advantages, such as injectability, ease of handling, and cost-effectiveness. However, with its application in the clinic, some disadvantages have been found, including cytotoxicity, high polymerization temperature, high elastic modulus, and high compressive strength. To improve the mechanical properties and the biological performance of conventional PMMA bone cement, several studies have modified it by adding bioceramics, bioglass, polymer materials, nanomaterials, and other materials, which have exhibited some advantages. In addition, other alternative materials, such as calcium phosphate, calcium sulfate, and calcium silicate cements—including their modifications—have also been explored. In this review, we examined the existing research on the side-effects of conventional PMMA bone cement, modified PMMA bone cement, and other alternative materials designed to improve properties in OVCFs. An overview of various modified bone cements can help further scientific research and clinical applications.

Favorable osteogenic activity of iron doped in silicocarnotite bioceramic: In vitro and in vivo Studies.

BackgroundCalcium phosphate silicate (Ca5(PO4)2SiO4 or CPS) is a promising bioceramic for bone grafting. Iron (Fe) is a trace element in the human body that has been reported to enhance the mechanical strength of CPS ceramics. However, the exact biofunctions of Fe, combined with another human trace element, viz. silicon (Si), in CPS and the optimal dose for Fe addition must be further investigated.MethodsIn vitro: the morphology, structure and cell adhesion were observed by SEM; the ability to promote osteogenic differentiation and mineralization was explored by ALP and alizarin red staining; the expression of osteogenic-specific genes and proteins was detected by PCR, WB and immunofluorescence. In vivo: Further exploration of bone regeneration capacity by establishing a skull defect model.ResultsIn vitro, we observed increased content of adhesion-related proteins and osteogenic-related genes expression of Fe-CPS compared with CPS, as demonstrated by immunofluorescence and polymerase chain reaction experiments, respectively. In vivo micro-computed tomography images, histomorphology, and undecalcified bone slicing also showed improved osteogenic ability of Fe-CPS bioceramics.ConclusionWith the addition of Fe2O3, the new bone formation rate of the Fe-CPS scaffold after 12 weeks increased from 9.42% to 43.76%. Moreover, both in vitro and in vivo experimental outcomes indicated that Fe addition improved the CPS bioceramics in terms of their osteogenic ability by promoting the expression of osteogenic-related genes. Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability.The translational potential of this articleThis study suggests that Fe-CPS bioceramics can be employed as a novel material for bone tissue engineering on account of their outstanding new bone formation ability,which provides promising therapeutic implants and strategies for the treatment of large segmental bone defects.

Comparative evaluation of incorporation calcium silicate and calcium phosphate nanoparticles on biomimetic dentin remineralization and bioactivity in an etch-and-rinse adhesive system.

This study aimed to evaluate the remineralization potential and bioactivity of adhesives, containing amorphous calcium phosphate (ACP) and calcium silicate (CS) nanoparticles (NPs). In this study, dentin slices (n=60) were prepared and etched with phosphoric acid. Next, they were divided into two groups: pre- and post-immersion in a simulated body fluid (SBF) for three weeks. The two groups were also divided into five subgroups (n=6 per subgroup), including the control (0 wt.% NPs); adhesives containing 1 wt.% and 2.5 wt.% (CS) nanoparticles; and adhesives containing 1 wt.% and 2.5 wt.% ACP nanoparticles. The remineralization potential and bioactivity of the adhesives were evaluated. The shear bond strength of the samples (n=18) was also assessed using a universal testing machine. The present results revealed that the adhesive containing ACP and CS nanoparticles showed bioactivity and remineralization potential without any reduction in the bond strength. The outcomes revealed that Cs and ACP nanoparticles induced mineralization in the dentin and incorporation of these nanoparticles to dentin bonding agents could improve the bio-functionalization of dentin bond. Key words:Calcium phosphate, calcium silicate, fourier transform infrared spectroscopy, scanning electron microscopy, tooth remineralization.