The Cryogenian Period (717–635 Ma), or ‘Snowball Earth’, was an unusually cool period of Earth history when glaciers extended to low latitudes. Past ideas on causes of this widespread glaciation include increased consumption of atmospheric carbon dioxide by silicate weathering due to continental drift into tropical paleolatitudes, or by voluminous, easily weathered volcanic tuffs. Alternatively, carbon sequestration from the atmosphere may have been intensified by advances in biomass on land or at sea. These hypotheses are tested with a new study of red siltstones of the Johnnys Creek Formation (785–717 Ma) in central Australia, where paleosols have long been recognised. Although these dolomitic red siltstones look like shales, they lack lamination. Instead, they have the massive bedding and grainsize distribution of dolomitic and calcareous loess, which precede tillites of the Areyonga Formation. Paleomagnetic studies indicate little drift from a paleolatitude of 26.2° during accumulation of the Johnnys Creek Formation. Nor does the Johnnys Creek Formation contain easily weathered volcanic ash, only local basalt flows. Paleoproductivity of the paleosols increases up section, as estimated in ppm soil CO2 from depth in paleosols to gypsic (By) and then calcic (Bk) horizons. Deepening and intensification of soil respiration reflects greater terrestrial carbon sequestration, and increased chemical weathering up section, and both would have drawn down atmospheric CO2. Comparable transition from gypsic to calcic soils in modern deserts reflects change from cyanobacterial-gypsic to fungal–algal calcic ecosystems. Snowball Earth glaciation may have been induced by evolutionary advances to eukaryotic and multicellular life on land, in the same way as Ordovician glaciation was induced by land plants, Permo-Carboniferous glaciation by trees, and Pleistocene glaciation by grasslands. KEY POINTS Johnnys Creek Formation of central Australia has paleosols dated 785–717 Ma. Little drift from paleolatitude 26.2°, and no volcanic ash, only local basalt flows. Paleosol paleoproductivity and depth of weathering increased steadily up section. Glaciation may have been induced by eukaryotic and multicellular life on land.