Caesalpinia Spinosa Research Articles

Influence of Molasses and Caesalpinia spinosa Meal Inoculums on Biogas Production from Cattle Manure

The management of organic waste through anaerobic digestion is an alternative to energy recovery. This research focused on evaluating the production of biogas with different inoculums. For this purpose, two types of systems were implemented—one used a heating system controlled by an STC-1000 thermostat, while the other used a solar heating system under a polycarbonate parabolic trough. The experiment was carried out at laboratory level with 3 L PET bottle biodigesters and the biogas produced was collected with the water displacement technique in 3 L bottles, calibrated every 50 mL, over 43 days. Inoculums of the following manure concentrations were used: water (1:5, 1:2, 1:3) mixed with Caesalpinia spinosa meal and molasses. The results determined that the thermostat-controlled heating system generated 69.6 mL/day of biogas while the other system produced 610.9 mL/day. On the other hand, the T1 treatment with a manure:water ratio of 1:5 and molasses and Caesalpinia spinosa meal inoculums in both systems had a higher average biogas volume. In terms of methane (CH₄), the highest value of 76.9% was obtained through the T1 treatment under the controlled heating system. This allows the production of biogas with a high concentration of methane, which in future applications can be utilized for residential or industrial purposes, promoting economic, social and environmental development. Since the main challenge in the production of biogas is to reduce the digestion time, which is influenced by the temperature of the site, two types of inoculums with a low cost and easy access were used.

DNA Barcoding Unveils Novel Discoveries in Authenticating High-Value Snow Lotus Seed Food Products.

Snow Lotus Seed (SLS), esteemed for its nutritional and market value, faces challenges of authentication due to the absence of appropriate testing standards and methods. This results in frequent adulteration of SLS sourced from Gleditsia sinensis (G. sinensis) with other plant seeds endosperm. Traditional chloroplast DNA barcoding methods are inadequate for species identification due to the absence of chloroplasts in G. sinensis seeds endosperm. In this study, the homology of 11 ITS genes among 6 common Gleditsia species was analyzed. Universal primers suitable for these species were designed and screened. A DNA barcoding method for distinguishing SLS species was developed using Sanger sequencing technology, leveraging existing GenBank and Barcode of Life Data System (BOLD) databases. Optimized sample pretreatment facilitated effective DNA extraction from phytopolysaccharide-rich SLS. Through testing of commercial SLS products, the species origin has been successfully identified. Additionally, a novel instance of food fraud was uncovered, where the Caesalpinia spinosa endosperm was used to counterfeit SLS for the first time. The study established that the developed DNA barcoding method is effective for authenticating SLS species. It is of great significance for combating food fraud related to SLS, ensuring food safety, and promoting the healthy development of the SLS industry.

Eco-friendly fabricating Tara pod extract-soy protein isolate film with antioxidant and heat-sealing properties for packaging beef tallow

Plastic pollution and its detrimental environmental effects have become a global concern. In this study, biodegradable packaging materials were developed by incorporating Tara pod extract (TPE) into soy protein isolate (SPI) films. TPE-SPI films exhibited improved mechanical properties, UV-blocking and heat-sealing performances, and barrier properties. The non-covalent interaction of SPI and Tara tannins enriched in TPE increased the films' tensile strength (TS). At 10% TPE content, the TS of the composite films increased by 56.4%, while the elongation at break (EB) reduced by 26.2%, in comparison to the pure SPI film. With 6% TPE, the composite film efficiently blocked UV lights. The oxygen permeability (OP) of 8TPE-SPI film (0.99 cm3 mm m−2 day−1 atm−1 × 10−9) was significantly lower than that of polyethylene (PE) film (59.5 cm3 mm m−2 day−1 atm−1 × 10−9). At heat-sealing conditions (120 °C, 2.0s, and 0.3Mpa), beef tallow packaged in 8TPE-SPI film showed an increase in POV from 0 to 0.002 g/100 g over 90 days, which was much lower than that of in PE pouches (0.0835 g/100 g). These findings highlight TPE-SPI films have potential to be applied in packaging oily foods.

Identification of polyphenols by UPLC-MS/MS and their potential in silico antiviral activity from medicinal plants in Trujillo, Peru

Context: Given the growing public health crisis caused by viral diseases, traditional medicine stands as one of the fundamental pillars for the study and discovery of phytometabolites with antiviral properties. It is necessary to investigate and evaluate these compounds, mostly phenolic, that could be used as drugs. Aims: To identify the polyphenols by UPLC-MS/MS and the potential in silico antiviral activity of medicinal plants in Trujillo, Peru. Methods: The ethnobotany of the eight most widely used medicinal plants in the city of Trujillo was described (Azadirachta indica A. Juss. “paradise”, Caesalpinia spinosa (Molina) Kuntze “tara”, Citrus limon (L.) Osbeck “lemon”, Clinopodium pulchellum (Kunth) Govaerts "panizara", Cordia lutea Lam. "overo", Ocimum basilicum L. "basil", Schinus molle L. "molle", and Taraxacum campylodes G.E. Haglund "dandelion"). The phytometabolites responsible for the antiviral activity were identified by LC-MS and evaluated in silico against the viral proteins NS2B/NS3 (DENV-2), NS5B (HCV), and ICP27 (HSV-1) by molecular docking using Chimera 1.16 software and molecular interaction by Maestro 13.1 software to identify the position and type of interaction. Results: Five polyphenols (chlorogenic acid, gallic acid, caffeic acid, rosmarinic acid, and rutin) were found, and in the in-silico test, the antiviral activity of chlorogenic acid stood out against DENV-2 and HCV, rutin against HCV and HSV-1, rosmarinic acid against DENV-2 and HCV. Conclusions: It is demonstrated that the medicinal plants studied contain phytometabolites that make them possible antiviral candidates, which would support their use in the traditional medicine of the province of Trujillo, Peru.

Comprehensive molecular level characterization of protein- and polyphenol-rich tara (Caesalpinia spinosa) seed germ flour suggests novel hypothesis about possible accidental hazards

Tara (Caesalpinia spinosa, Leguminosae) seed germ (TSG), a by-product of tara gum (E417) extraction, has been used as a protein- and polyphenol-rich food ingredient for human and animal nutrition. Nevertheless, TSG is the alleged culprit for a recent foodborne outbreak of even severe acute illnesses that have affected hundreds of individuals in the USA, perhaps triggered by nonprotein amino acids such as baikiain. Herein, the composition of TSG has been characterized at molecular level, with a focus on proteins, phenolics, lipids, and mineral composition. TSG contains 43.4 % (w/w) proteins, tentatively identified for the first time by proteomics, and 14 % lipids, consisting of 83.6 % unsaturated fatty acids, especially linoleic acid. Ash is surprising high (6.5 %) because of an elevated concentration of P, K, Ca, and Mg. The detection of a rare earth element such as gadolinium (Gd, 1.6 mg kg−1), likely sourced from anthropogenic pollution, suggests alternative hypotheses for the origin of TSG hazards.

Sustainable production strategies to increase the commercialization of tara in Peru

Tara (Caesalpinia spinosa) is a native Peruvian plant that grows in the dry forests of the inter-Andean valleys. It is considered a relevant opportunity for eco-business in the country due to its high efficiency of use and versatility in various industries. This study aims to characterize the production and commercialization of tara by producers located in the Sonche River Valley, in the province of Chachapoyas, in order to provide some opportunities for implementing sustainable tara production strategies to increase its production. The results indicate that tara production activity is being developed in a precarious manner, since production per hectare is below average and there is inefficient use of spaces suitable for cultivation; the production units also have organizational deficiencies such as lack of leverage, little training on sustainability issues and neglect in the harvesting process. These deficiencies are invaluable opportunities to implement strategies such as the practice of sustainable crops, the use of improved varieties, and education, research and development regarding tara production.

Molecular characterization of a galactomannan extracted from Tara (Caesalpinia spinosa) seeds

Tara gum (TG) is a polysaccharide extracted from the seeds of a South American tree called Tara (Caesalpinia spinosa). TG is a galactomannan with many applications in the food industry, mainly as an emulsifier and stabilizer agent. In addition, it is also used in the paper and cosmetic industries. In the present study, we performed a molecular characterization based on chemical composition and physicochemical properties to understand the properties behind TG applications. TG was extracted and purified from Tara seeds distributed in different ecoregions of Bolivia. The monosaccharide composition analysis was determined by high-performance anion-exchange chromatography/pulsed amperometric detection (HPAEC-PAD). At the same time, their molecular characteristics, such as molar mass, root-mean-square radius, hydrodynamic radius, conformation, and densities, were studied by asymmetrical flow field-flow fractionation coupled to multi-angle light scattering refractive index (AF4-MALS-dRI), also the specific refractive index increment (dn/dc) was determined for the first time using AF4 for TG. The results revealed that the gum samples are galactomannans composed of mannose (Man) and galactose (Gal) in a ratio of 3.37 (Man/Gal), with an average molar mass range from 2.460 × 107 to 3.699 × 107 Da, distributed in a single population. The root-mean-square radius range from 260.4 to 281.6 nm, and dn/dc is 0.1454. The Kratky plots based on 14 scattering angles indicated that the conformation of all samples corresponds to random coil monodisperse, while their gyration radius/hydrodynamic radius ratio (ρ) is high. All these results suggest that the chains have a low branched density, consistent with the Gal/Man composition. To the best of our knowledge, we report for the first time an integrated physicochemical study of TG relevant to developing emulsifier and stabilizer formulations.

Plant extracts modulate cellular stress to inhibit replication of mouse Coronavirus MHV-A59

The Covid-19 infection outbreak led to a global epidemic, and although several vaccines have been developed, the appearance of mutations has allowed the virus to evade the immune response. Added to this is the existing risk of the appearance of new emerging viruses. Therefore, it is necessary to explore novel antiviral therapies. Here, we investigate the potential in vitro of plant extracts to modulate cellular stress and inhibit murine hepatitis virus (MHV)-A59 replication. L929 cells were treated with P2Et (Caesalpinia spinosa) and Anamu SC (Petiveria alliacea) plant extracts during infection and virus production, ROS (reactive oxygen species), UPR (unfolded protein response), and autophagy were assessed. P2Et inhibited virus replication and attenuated both ROS production and UPR activation induced during infection. In contrast, the sustained presence of Anamu SC during viral adsorption and replication was required to inhibit viral infection, tending to induce pro-oxidant effects, and increasing UPR gene expression. Notably, the loss of the PERK protein resulted in a slight decrease in virus yield, suggesting a potential involvement of this UPR pathway during replication. Intriguingly, both extracts either maintained or increased the calreticulin surface exposure induced during infection. In conclusion, our findings highlight the development of antiviral natural plant extracts that differentially modulate cellular stress.

Utilization of Guarango (Caesalpinia spinosa) in the Province of Loja

Guarango (Caesalpinia spinosa) was studied in different parts of Loja province, mainly Loja, Catamayo, Gonzanamá, and Paltas, to use this species in the food and chemical industries because many studies in our country show that this species is not correctly used. It started with studies of the yields according to the place and time of recollection, after which the correct treatment was found, like the kind of grinding to optimize the handling of the sample, the chemical determination (tannins) in the pod, and its bromatological characteristics (moisture, fat, and protein). The chemical determination of tannins was done through titration of a macerated pod using carmine indigo and KMnO4 to 0,1 N, while bromatological determination with moisture, fat, and protein was applied using the standard methods of AOAC 7003, AOAC 7.056 y AOAC 47.021, respectively. In these results, it was found that there is little variability in experiments of percent of integument, endosperm, and germ, while in experiments of moisture, the amount of pod and seeds had significant differences. In addition, the percentage of tannins was excellent for use in the leather industry. While the germen had qualities of a good food supplement due to its high fat and protein content and low moisture. Finally, a method of extraction of the guarango parts could be designed that can be easily replicable by farmers. Based on the results, we can conclude that it is possible to establish ventures with guarango (C. spinosa) due to the remarkable properties of this species in food, textiles, and other industries.
 Keywords: Caesalpinia spinosa, guarango, pod, germ, used.
 Resumen 
 Se realizó un estudio sobre el guarango (Caesalpinia spinosa) de diferentes cantones de la provincia de Loja, principalmente: Loja, Catamayo, Gonzanamá y Paltas, con la finalidad de aprovechar esta especie ofreciendo alternativas de uso dentro de la industria química y alimentaria ya que, según estudios realizados, dentro de nuestro país no es aprovechada. Se partió desde el estudio de rendimientos según el lugar y tiempos de recolección, posterior a esto se buscó el tratamiento adecuado como molienda para el manejo óptimo de la muestra, la determinación química (taninos) en vaina y bromatológica (humedad grasa y proteína). Para la determinación química (taninos) se realizó titulación al macerado de la vaina usando índigo carmín y KMnO4 al 0,1 N, mientras que para determinación bromatológica como la humedad, grasa y proteína se aplicó métodos estandarizados de la AOAC 7003, AOAC 7.056 y AOAC 47.021, respectivamente. En los resultados se obtuvo ensayos que demostraron la poca variabilidad del porcentaje de tegumento, endospermo y germen, mientras que en los ensayos de humedad, cantidad de vainas y semillas, taninos, hay variabilidades significativas.

Atmospheric immersion and vacuum impregnation of gallotannins and hydrolysed gallotannins from tara pods (Caesalpinia spinosa) mitigate acrylamide and enhances the antioxidant power in potato chips

This research aimed to evaluate atmospheric pressure immersion (API) and vacuum impregnation (VI) of non-hydrolyzed and hydrolyzed tara gallotannins (TE and THE) extracts and the purification process by leaching with ethyl acetate (TE-L and THE-L) and absorption chromatography on the acrylamide (AA) mitigation in potato chips. Better results were obtained with THE-L at hydrolysis degrees of 48.5 and 99.8 % and API with AA reduction of 57.9 and 61.7 %, respectively (p > 0.05), while with TE-L, AA reduction was 29.2 %. Instead, THE-L and TE-L with VI reduced AA by 70.3 and 66.4 %, respectively. In potato chips subjected to THE-L treatment, phenolic compounds and ABTS AC increased in 86.5–91.0 % and 71.0–103.2 %, respectively compared to the control. The main antioxidants were identified in the extracts and in potato chips. Tara gallotannins are an interesting alternative to mitigate AA formation and to enhance the antioxidant power of potato chips.

Plant-derived extracts P2Et and Anamu-SC affect NO and ROS levels in leukemic cells

Leukemic cells often show high nitric oxide (NO) and reactive oxygen species (ROS) levels. These can lead to resistance to apoptosis and therapy and increased proliferation. Plant-derived extracts decrease chemoresistance in cancer cells. In this study, we evaluated the effects of the plant-derived extracts P2Et (Caesalpinia spinosa) and Anamu-SC (Petiveria alliacea) and their combination with chemotherapeutic agents on NO and ROS levels in leukemic cell lines K562 and Reh. NO and ROS were determined using the DAF-FM DA and H2DCFDA probes. The mean fluorescence intensity for each variable was measured by flow cytometry. The extracts showed an antioxidant effect on both cell lines leading to a significant decrease in ROS levels without decreasing cell viability. Anamu-SC also increased NO levels in K562 cells when combined with idarubicin. Both extracts reduced the number of leukemic cells after 12 hours of treatment. Further studies are necessary to evaluate their effect on primary human leukemia cells. These findings suggest the potential of P2Et and Anamu-SC as adjuncts in leukemia treatment.

Doxorubicin Activity Is Modulated by Traditional Herbal Extracts in a 2D and 3D Multicellular Sphere Model of Leukemia.

The modulation of the tumor microenvironment by natural products may play a significant role in the response of tumor cells to chemotherapy. In this study, we evaluated the effect of extracts derived from P2Et (Caesalpinia spinosa) and Anamú-SC (Petiveria alliacea) plants, previously studied by our group, on the viability and ROS levels in the K562 cell line (Pgp- and Pgp+), endothelial cells (ECs, Eahy.926 cell line) and mesenchymal stem cells (MSC) cultured in 2D and 3D. The results show that: (a) the two botanical extracts are selective on tumor cells compared to doxorubicin (DX), (b) cytotoxicity is independent of the modulation of intracellular ROS for plant extracts, unlike DX, (c) the interaction with DX can be influenced by chemical complexity and the expression of Pgp, (d) the 3D culture shows a greater sensitivity of the tumor cells to chemotherapy, in co-treatment with the extracts. In conclusion, the effect of the extracts on the viability of leukemia cells was modified in multicellular spheroids with MSC and EC, suggesting that the in vitro evaluation of these interactions can contribute to the comprehension of the pharmacodynamics of the botanical drugs.

Antimicrobial Potential of Tara Hydroalcoholic Extract (Caesalpinia spinosa) against Streptococcus Associated with Strep Throat

Antibiotics are often prescribed to treat infections caused by group B Streptococcus; however, inappropriate use of antibiotics can develop resistance. Because of this, the research was carried out with the aim of evaluating the in vitro effect of the hydroalcoholic extract of Caesalpinia spinosa (Molina) Kuntze known as Taya or Tara on the viability of β-hemolytic streptococci; an experimental investigation of increasing stimulation was carried out. The hydroalcoholic extract of C. spinosa pods was worked in concentrations of 250, 500, 750, and 1000 mg/mL, which were placed on filter paper discs to perform the sensitivity test following the Kirby–Bauer method. The greatest inhibition of bacterial viability was observed in the penicillin control group (GPT-01) followed by the TCT-04 group (hydroalcoholic Tara extract 1000 mg/mL). In addition, it was found that these groups are statistically different from the rest (p < 0.05), while the lowest bacterial inhibition was obtained for the erythromycin group and the TCT-01 group (250–1000 mg/mL). It was concluded that the hydroalcoholic extract of the pods of C. spinosa (Molina) Kuntze affects the viability of β-hemolytic streptococci associated with strep pharyngitis and that this antimicrobial activity is due to the presence of tannins, steroidal flavonoid, and alkaloids. Likewise, the tested concentrations of hydroalcoholic Tara extract were found to have better antibacterial activity than erythromycin (15 µg/mL) against β-hemolytic streptococci. These results are hopeful for the traditional or herbal medicine field. However, future in vivo research is needed to determine its effectiveness in humans.

Plant extract from Caesalpinia spinosa inhibits cancer-associated fibroblast-like cells generation and function in a tumor microenvironment model

Interactions in the tumor microenvironment (TME) between tumor cells and stromal cells such as cancer-associated fibroblasts (CAF) favor increased survival, progression, and transformation of cancer cells by activating mechanisms of invasion and metastasis. The design of new therapies to modulate or eliminate the CAF phenotype or functionality has been the subject of recent research including natural product-based therapies. We have previously described the generation of a standardized extract rich in polyphenols obtained from the Caesalpinia spinosa plant (P2Et), which present antitumor activities in breast cancer and melanoma models through activities that modulate the metabolism of tumor cells or induce the development of the immune response. In this work, a model of CAF generation was initially developed from the exposure of 3T3 fibroblasts to the cytokine TGFβ1. CAF-like cells generated in this way exhibited changes in the expression of Caveolin-1 and α-SMA, and alterations in glucose metabolism and redox status, typical of CAFs isolated from tumor tissues. Then, P2Et was shown to counteract in vitro-induced CAF-like cell generation, preventing caveolin-1 loss and attenuating changes in glucose uptake and redox profile. This protective effect of P2Et translates into a decrease in the functional ability of CAFs to support colony formation and migration of 4T1 murine breast cancer tumor cells. In addition to the functional interference, the P2Et extract also decreased the expression of genes associated with the epithelial-mesenchymal transition (EMT) and functional activities related to the modulation of the cancer stem cells (CSC) population. This work is an in vitro approach to evaluate natural extracts’ effect on the interaction between CAF and tumor cells in the tumor microenvironment; thus, these results open the chance to design a more profound and mechanistic analysis to explore the molecular mechanisms of P2Et multimolecular activity and extent this analysis to an in vivo perspective. In summary, we present here a standardized polymolecular natural extract that has the potential to act in the TME by interfering with CAF generation and functionality.

Increased Production of Tara (Caesalpinia spinosa) by Edaphoclimatic Variation in the Altitudinal Gradient of the Peruvian Andes

Tara production occurs mainly in the altitude gradient, where the edaphoclimatic conditions that affect the production of pods still need to be understood. The goal was to determine the altitudinal and edaphoclimatic effect on the production of tara pods in agroforestry and natural remnants in Cajamarca, Peru. Data analyses performed were the following: principal component analysis (PCA), regression analysis, the bootstrap method, and Pearson correlation analysis. For each 1 °C increase in temperature, the length and width of the pod decreased by 2.1 and 0.62 mm, and of the seed by 0.17 and 0.12 mm in the agroforestry environment; likewise, pod, valve, seed and gum weights were reduced by 23.9, 10.9, 13 and 2.3 g in the agroforestry environment, and 22.3, 13, 9.3 and 2.1 g in the natural environment. Activities such as association with annual crops and perennial pasture possibly favor the length and width of the pod and seed and the weight of the pod, valve, seed and gum in the agroforestry environment when compared to the natural environment. Larger pod and seed dimensions and higher pod, valve, seed and gum weights are related to higher soil CaCO3 contents in the natural environment and higher soil P and B contents in the agroforestry environment at higher altitudes. Higher Fe contents in the soil suggest an improvement in tara’s tannin weight (valve) in the natural and agroforestry environment. The effective response of tara, reflected in its weight and size of pods, was higher in an agroforestry environment than in a natural environment. Further studies on the production of tara pods are necessary for a better understanding of the interaction between altitude and soil fertility to expand the revenue and employment of Peruvian tara farmers.

Tara (Caesalpinia spinosa) in Natural and Agroforestry Systems under an Altitudinal Gradient in the Peruvian Andes: Responses to Soil and Climate Variation

This research examined how edaphoclimatic variations are related to dendrometric variables of the Tara tree in natural and agroforestry systems in Cajamarca, Peru. Evaluations followed three approaches: (a) principal components analysis (PCA) with environmental factors and altitude classes with 1 °C of temperature variation for edaphic and dendrometric variables; (b) evaluation of possible differences by the bootstrap method for the different variables in the PCAs; and (c) correlation analysis between plant density, plant and crown height, stem and crown diameter, and the number of stem branches with the physical and chemical attributes of the soil and with air temperature. In the altitudinal gradient from 2021 to 3007 ± 7 m, the temperature ranged from 19.8 to 13.4 ± 0.4 °C; the soils possessed alkaline pH, high organic matter (OM), K and CEC, lower contents of CaCO3, N, P, K, B, Cu, Fe, Mn, and Zn. The soil properties with the most significant contribution to PCAs were OM, CEC, N content, and sand, with no variation among environments but among altitudes. None of the dendrometric variables varied as a function of altitude and temperature in PCAs run in the natural environment. However, in the agroforestry environment, there was a greater crown diameter, and tree and crown height in the 2185 m altitude class associated with Medicago sativa. In contrast, the opposite behavior was found in these variables and in the altitude class 2798 m associated with low Fe content, and already in the altitude class at 3007 m, a larger stem diameter is associated with higher levels of CaCO3. The anthropic effect on the agroforestry environment did not significantly alter the soil’s CEC, OM, N, and sand. Tara’s crown diameter and tree height appeared higher in agroforestry environments. The variations of Fe and CaCO3 in the soil might have influence on the development of Tara individuals in natural and agroforestry environments. It is important to carry out further studies for a better understanding of the relationship between the production of Tara pods and soil fertility in altitudinal variation, aiming to improve the income and employment of family farmers who exploit Tara in the Peruvian Andes.

Effect of water deficit on water status and growth of five tropical species used in urban forestry

Due to the urban environment urban trees must deal with drier and hotter than in rural areas. Knowing the water consumption of each species and the indicators of water deficit is useful to decide the frequency and volume of irrigation and to select species according to the microclimate of the location. To determine approximately the irrigation frequency and to identify physiological variables that indicate water stress, it was carried out an experiment in which five tropical species (Citharexylum montanum M., Citharexylum sulcatum M., Caesalpinia spinosa K., Inga edulis M. and Retrophyllum rospigliosii P.) were subjected to water deficit. After a month of planting, eight trees per species were subjected to four treatments: control treatment (volumetric water content higher than 45% (TC)), the volumetric water content of 20% (VM20), fifteen and thirty days after the soil had reached VM20 (T15 and T30, respectively). In trees with similar height, it was found that the descending order of water consumption was I. edulis, C. montanum, C. spinosa, C. sulcatum, R. rospigliosii and that the best indicator of water deficit was the stem water potential. In general, volumetric moisture of soil of 20% was a suitable threshold to decide when irrigating regardless of the species. Deeming the effect of the treatments on the growth of the assessed species, T30 diminished severely the growth by 50% in comparison to the control, except for C. sulcatum in which there were no significant differences.

Combination of simultaneous extraction–hydrolysis and intermittent feeding of tara pod for efficient production of gallic acid

The pods of Caesalpinia spinosa consists of gallotannins, which represent more than 50 % of its weight. The traditional chemical procedure used to produce gallic acid (GA) from tara gallotannins is a high-pollution process. Although biological procedures, including microbial fermentation and enzymatic hydrolysis, are environmentally friendly, insufficient production efficiency significantly hinders industrial application. In this study, we proposed a novel strategy for the simultaneous extraction and enzymatic hydrolysis of tara gallotannins with intermittent feeding. A recombinant thermostable tannase (Tan1) derived from Aspergillus oryzae was first constructed to adapt to the high-temperature simultaneous extraction and enzymatic hydrolysis process. Tan1 showed competent activity yield (83.11 U/mL) and excellent heat resistance, exhibiting the highest activity at 80 °C and half-lives at 60 °C for more than 6 h. The production efficiencies of GA using different enzymatic hydrolysis strategies were compared. The simultaneous extraction and enzymatic hydrolysis strategy combined with intermittent feeding minimized the inhibition of tara gallotannins on Tan1 activity and resulted in an increased GA yield. Using this strategy, 192 g/L tara powder (intermittent feed for 6 times) was treated with Tan1 at 60 °C for 177 min, resulting in 78.5 g/L GA output which was the highest among the currently reported values obtained using biological protocols for GA production.

Effect of the Integrated Addition of a Red Tara Pods (Caesalpinia spinosa) Extract and NaCl over the Neo-Formed Contaminants Content and Sensory Properties of Crackers.

A 2k factorial design with three centrals points was considered to evaluate the effect of adding red Tara pods extract (Caesalpinia spinosa) (440–2560 µg/mL of dough water) and NaCl (0.3–1.7 g/100 g of flour) on the acrylamide (AA) and hydroxymethylfurfural (HMF) content and sensory attributes of crackers. Additionally, the best formulation, defined as that with the lowest AA and HMF content, was compared with a commercial formulation cracker. Red Tara pods extracts were obtained through conventional extraction using pure water (60 °C, 35 min). AA and HMF content were quantified by GC-MS and HPLC-DAD, respectively. The sensory evaluation was carried out using a descriptive analysis on a 10 cm non-structured linear scale. Red Tara pods extract significantly reduced (p < 0.05) the AA and HMF content, while NaCl only influenced the HMF formation. However, the sensory attributes did not significantly change (p > 0.05), excepting the violet-gray color and salty flavor, but at acceptable levels compared with the control sample. The higher the red Tara pods extract concentration (2560 µg/mL of dough water), the lower the neo-formed contaminants (NFCs) content of crackers (AA: 53 µg/kg and HMF: 1236 µg/kg) when salt level was below 3 g/100 g of flour. The action of the proanthocyanidins present in the extracts which trapped the carbonyl groups of sugars probably avoided the formation of both NFCs. Contrarily, NaCl addition (from 0.3 to 1.7 g/100 g of flour) significantly increased (p < 0.05) the HMF formation (from 1236 µg/kg to 4239 µg/kg, respectively), probably through the dehydration of carbohydrates during the Maillard reaction. When explored treatments were compared with a commercial formulated cracker, the highest mitigation effect (reductions of 40% and 32% AA and HMF, respectively) was reached with the addition of 2560 µg/mL of dough water of red Tara pods extract and 0.3 g/100 g of flour of NaCl. The addition of red Tara pods extracts integrated with the control of NaCl levels mitigated the NFCs in crackers, preserving their sensory properties. Future research should be focused on scaling this mitigation technology, considering a better chemical characterization of red Tara pods extracts as well as the validation of its use as functional food ingredient.

Flavonoids from Chromolaena leivensis and Caesalpinia spinosa as potential protectors against doxorubicin-induced cardiomyopathy

Flavonoids from Chromolaena leivensis and Caesalpinia spinosa as potential protectors against doxorubicin-induced cardiomyopathy