Cadmium Levels In Liver Research Articles

Survey for Cadmium Concentration in Liver, Kidney and Muscle of Slaughtered Sheep in Butcher Shops of Fallujah City, Iraq

Cadmium (Cd) is regarded as one of major the presence of hazardous substances in the environment, with a broad variety toxicity to organs and an extended half-life of removal. Cd exposure for farmed ruminants can come from industrial processing and intensive agricultural operations, which can pollute feed and water. It was noted that most of the wastes produced by nearby factories in Al Fallujah city are either strewn around the highways or use as landfills while the sewage is used for irrigation, while sheep graze on the edges of runways and other sites that might have been contaminated with hazardous materials. EF (enrichment factor) According to the findings, the concentration of Cd in the soils of Al Fallujah city was exceptionally high, and according to sediment content criteria, the soils in Fallujah city were contaminated with high concentrations of Cd, revealing the influence of human and industrial activity on Cd accumulation in the soil. Cadmium levels in liver, kidney and muscle from 216 samples for 72 slaughtered sheep in slaughterhouse at Al Fallujah city, Iraq during the period of October to December 2020 were analyzed. The values of Cadmium detected in sheep liver, kidney and muscle samples were 0.138± 0.020, 0.432 ± 0.090, and 0.037± 0.003 mg/kg, respectively which refer to high concentration of Cd in sheep viscera also This study demonstrates the need of assessing and monitoring cadmium and other hazardous metal levels in meals in order to create population-safe intake values.

Early-Life Exposure to Low-Dose Cadmium Accelerates Diethylnitrosamine and Diet-Induced Liver Cancer.

Maternal exposure to cadmium causes obesity and metabolic changes in the offspring, including nonalcoholic fatty liver disease-like pathology. However, whether maternal cadmium exposure accelerates liver cancer in the offspring is unknown. This study investigated the impact of early-life exposure to cadmium on the incidence and potential mechanisms of hepatocellular carcinoma (HCC) in offspring subjected to postweaning HCC induction. HCC in C57BL/6J mice was induced by diethylnitrosamine (DEN) injection at weaning, followed by a long-term high-fat choline-deficient (HFCD) diet. Before weaning, liver cadmium levels were significantly higher in mice with early-life cadmium exposure than in those without cadmium exposure. However, by 26 and 29 weeks of age, hepatic cadmium fell to control levels, while a significant decrease was observed in copper and iron in the liver. Both male and female cadmium-exposed mice showed increased body weight compared to non-cadmium-treated mice. For females, early-life cadmium exposure also worsened insulin intolerance but did not significantly promote DEN/HFCD diet-induced liver tumors. In contrast, in male mice, early-life cadmium exposure enhanced liver cancer induction by DEN/HFCD with high incidence and larger liver tumors. The liver peritumor tissue of early-life cadmium-exposed mice exhibited greater inflammation and disruption of fatty acid metabolism, accompanied by higher malondialdehyde and lower esterified triglyceride levels compared to mice without cadmium exposure. These findings suggest that early-life exposure to low-dose cadmium accelerates liver cancer development induced by a DEN/HFCD in male mice, probably due to chronic lipotoxicity and inflammation caused by increased uptake but decreased consumption of fatty acids.

Sub-chronic ascorbic acid supplementation accelerates reduction of liver cadmium levels in broiler chicken

Sub-chronic ascorbic acid supplementation accelerates reduction of liver cadmium levels in broiler chicken

Lead and Cadmium Levels in Liver, Kidney and Muscle of Harvested Wild Springbok (Antidorcus marsupialis) Under Extensive Management in Southern and Southeastern Namibia

The aims of the study were to assess heavy metals (lead and cadmium) values in meat and offal from harvested springbok. For cadmium (Cd), mean concentrations in liver and kidney were 0.10 ± 0.04 mg/kg, 0.33 ± 0.05 mg/kg and 1.04 ± 0.15 mg/kg, 0.91 ± 0.12 mg/kg for lead (Pb). None of the samples had a Cd level above the limits set for the liver (0.5 mg/kg) and 1.0 mg/kg set for the kidney while two of the samples had a Pb level above the limits for the liver (0.5 mg/kg) and 12 samples had Pb values above the limits for the kidney (0.5 mg/kg). The mean Pb and Cd concentrations in muscles were below the detection limit. In the current study, interpretation of the results was limited by the small number of positive samples; however, values were within the range previously reported in literature for wildlife species elsewhere.

Cadmium levels in the lung, liver, kidney cortex, and urine samples from Australians without occupational exposure to metals.

The authors undertook this study to assess levels of cadmium exposure in the general population. Samples of lung, liver, and kidney were obtained from 61 cadavers (43 males, 18 females; 2-89 yr of age, mean age = 38.5 yr) who died from accidental causes and who were subject to postmortem examinations at the John Tonge Centre for Forensic Sciences, Queensland Health Scientific Services, Brisbane, Australia, in 1997 and 1998. Samples of bladder urine were also obtained from 22 cadavers. Tissue and urine samples were analyzed for cadmium, zinc, and copper with inductively coupled plasm (ICP) mass spectrometry. The overall mean values for cadmium in the lung, liver, and kidney cortex samples were 0.13, 0.95, and 15.45 microg/gm wet tissue weight. The average renal cadmium level in subjects with high lung-cadmium levels (n = 13) was 6 microg/gm wet tissue weight higher than that of similarly aged subjects who had medium lung-cadmium levels (n = 30). In females, the average level of cadmium in the liver was 74% greater than in males, and the average liver cadmium in females with high lung-cadmium levels was 100% higher than in males in the same age range who had the same high lung-cadmium levels. Renal cadmium accumulation tended to be greater in females than in males who were in the same age range and who had similar lung-cadmium levels, a result that suggested that there was a higher absorption rate of cadmium in females. The mean value for a urinary cadmium excretion of 2.30 microg/gm creatinine was found in a subset of samples that had a mean age of 39 yr and a renal cortex cadmium concentration of 18.6 microg/gm wet tissue weight. Urinary cadmium excretion rates were correlated more strongly with lung and kidney cadmium content than with age or liver cadmium levels. The results suggest that urinary cadmium excretion may be increased in smokers and could provide some estimate of body cadmium burdens in future Australian epidemiological studies.

Cadmium levels in liver, kidney and meat in calves from Asturias (North Spain)

The aim of the present study was to investigate the levels of cadmium in calves from Asturias and to compare them with the levels reported in other countries and with the maximum tolerance levels in products for human consumption. The Principado de Asturias contains a large mining area and, for that reason, may deserve special attention with regard to possible heavy metal contamination. Samples of liver, kidney and meat from 312 animals aged 6–12 months were collected from the whole region. Samples were acid digested and cadmium concentrations were determined by graphite furnace atomic absorption spectrophotometry (GFAAS). A strict analytical quality control was carried out during the study. The average wet weight concentrations in liver, kidney and meat were respectively 30.7 μg/kg, 161 μg/kg and 2.03 μg/kg and the levels found in the different tissues were comparable to those reported from other investigations carried out in different countries in recent years. Only 2.9% of kidneys exceeded the tolerance limits established in the strictest legislation, whereas no samples of liver and meat exceeded the limits.

In vivo cadmium mobilization by three novel bis(carbodithioates).

Four novel cadmium-chelating agents N,N'di(D-glucosyl)alkanediamine-N,N'-bis(carbodithioates) 4a-e were prepared as disodium salts of the general formula (CH2)n[N(CS2Na)CH2(CHOH)4CH2OH]2, where n = 7, 8, 10, and 12. They were synthesized by the reductive coupling of 2 mol of alpha-D-(+)-glucose (1) with 1 mol of the corresponding alkanediamines 2a-e followed by reaction with CS2 in a basic medium. The elemental analyses of the Cd complexes 5a, b, d, and e prepared revealed that these bis(carbodithioates) form 1:1 complexes with Cd2+. The in vivo cadmium-mobilizing efficacy of three of the new chelators (4a, b, and d) was determined in rats pretreated ip with CdCl2.H2O containing 2 microCi of 109Cd (74 kBq), and comparable data were obtained on a previously reported member of this series (4c, n = 9), and with BGDTC (sodium N-benzyl-D-glucamine-N-carbodithioate, 6), all given once at 1.0 mmol/kg ip. The compound with n = 12 (4e, C12G2DTC) proved to be too toxic in the preliminary study and was not evaluated further. A single injection of the 4a, b, and d caused a reduction in Cd levels of the whole body to ca. 50% and liver to ca. 12% of control levels. Comparable experimental data on BGDTC resulted in a reduction of whole-body cadmium to only 78% of the control levels and a reduction in liver cadmium to only 56% of control. After one and six injections at the same dosage, the new chelators (4a, b, and d) and C9G2DTC (4c, n = 9) significantly surpassed BGDTC for whole-body and liver Cd depletion but caused only a very modest depletion of renal Cd. While these compounds were designed to allow the two dithiocarbamate groups to coordinate to the same Cd2+ in vivo, the data do not prove that the two > NCS2Na groups bind to the same cadmium ion in vivo. The very rapid reduction in liver cadmium levels following only a single injection indicates that these -2 anions rapidly gain access to intracellular hepatic sites by some transport system.

A review of heavy metal and organochlorine levels in marine mammals in Australia

Study of toxic contaminants in marine mammal specimens collected around Australia is currently uncoordinated and piecemeal. Most states collect samples but there is little or no financial support for their analysis. This study combines data, published or unpublished, from 13 sources. Heavy metals have been analysed in about 676 specimens; over 400 were for mercury levels in P. macrocephalus taken at a whaling station. The remaining samples were mostly from toothed whales, a few baleen whales (< 20), pinnipeds (41) and dugongs (49). The most consistently analysed metals were lead, mercury and cadmium. Liver and kidney lead levels ranged from < 1–3 ppm; levels in bone were 0–418 ppm, withmost less than 10 ppm. Mercury levels in a large sample of P. macrocephalus muscle were < 12.2 ppm. Mercury levels in the small number of samples from other species were 0.51–143 ppm (kidney), 1.52–479 ppm (liver) and < 0.1–36 ppm (muscle). Cadmiumlevels in liver (0–52 ppm) and kidney (0–106 ppm) were extremely variable. Levels greater than 10 ppm were recorded in many species and were especially high in Hydrurga leptonyx, Dugong dugon, Mesoplodon layardii and Pseudorca crassidens. Adult Tursiops truncatus inhabiting the inshore gulfs of South Australia had considerably higher levels of cadmium compared with other regions. Information on organochlorine levels is sparse (approximately 39 specimens) and suggest low levels when compared to other parts of the world. Total DDT was highest (28.4 ppm) in a neonatal Orcinus orca. Some high levels of DDT were recorded in Tursiops truncatus, Delphinus delphis and Arctocephalus pusillus doriferus. PCBs ranged from < 0.05 to 3.87 ppm. A comprehensive pathological assessment of marine mammals is needed in order to evaluate the effects of toxic contaminants.

Seasonal variation in heavy metal levels in tissues of common guillemots, Uria aalge from northwest Scotland.

Mercury, cadmium, zinc, and copper concentrations were analyzed in three samples of common guillemot (in April, June, and November). Levels measured were uniformly low, and not enough to have any toxic effects. Adult guillemots had significantly more cadmium in their livers and kidneys than juveniles, with juvenile levels ranging from 25% to 89% of adult levels. Mercury concentrations in liver and kidney were also higher in adults. Juvenile levels represented from 80% to 94% of adults, but there were no age differences in feather and muscle mercury. Mercury levels declined throughout the year in internal tissues from April through June to November. There was a strong seasonal fluctuation in cadmium levels in liver and kidney, rising significantly between April and June and declining in both adult and juvenile birds. The influences of seasonal processes (namely breeding and moult) and seasonal dietary differences as causative factors in the changes in metal burdens are discussed. These findings have implications for the use of seabirds as monitors of heavy metals in the marine environment.

Biological monitoring for occupational cadmium exposure: the urinary metallothionein

The relationship between urinary metallothionein and kidney and liver cadmium levels was examined in 68 active and retired smelter workers. Metallothionein was analyzed by a radioimmunoassay and liver and kidney cadmium levels were determined by in vivo neutron activation. Four workers suffered from severe renal dysfunction and excreted high amounts of total protein and β 2-microglobulin and greater than 1 mg metallothionein/g creatinine. In the remaining 64 workers the urinary metallothionein levels correlated significantly with the cadmium levels in both liver and kidney. Similarly, in these individuals urinary metallothionein was significantly related to cadmium in blood and urine. These results demonstrate that urinary metallothionein is a sensitive biological indicator of cadmium exposure and body burden, before the onset of severe renal dysfunction.

Meso-2,3-dimercaptosuccinic acid and sodium N-benzyl-N-dithiocarboxy-D-glucamine as antagonists for cadmium intoxication.

Orally administered meso-2,3-dimercaptosuccinic acid (DMSA) is an effective antagonist for acute oral cadmium chloride (1 mmol/kg) intoxication in mice when administered up to 8 h after cadmium ingestion. Administration of sodium N-benzyl-N-dithiocarboxy-D-glucamine (NaB) i.p. along with DMSA p.o. resulted in kidney and liver cadmium levels only marginally smaller than those obtained with DMSA alone. Both chelation treatment regimens permitted survival of 80% or more of the animals, in comparison to a survival rate of 40-50% in untreated animals. Intraperitoneally administered NaB by itself is a very effective antagonist for cadmium chloride administered intraperitoneally in either acute or chronic cadmium intoxication. A dose-response study was made of the mobilization of cadmium from the liver and kidney of cadmium-loaded mice by NaB; this showed that NaB is one of the most effective cadmium mobilizing agents developed to date. We have also confirmed the earlier report of Kojima and his co-workers of the ability of NaB to remove cadmium from animals which have been treated with cadmium over an extended period of time. NaB causes a very large increase in the biliary excretion of cadmium. Nuclear magnetic resonance (NMR) spectra of 113Cd in bile from treated animals and model solutions indicates that such cadmium is undergoing rapid ligand exchange.

Use of the Topliss scheme for the design of more effective chelating agents for cadmium decorporation.

The Topliss scheme, which designates a special series of substituents for use in sequence in the search for suitably substituted aromatic compounds with biological properties superior to the parent unsubstituted aromatic compound, has been applied to the design of new chelating agents for the decorporation of cadmium from its deposits in vivo. Substituted benzylamines have been used in the syntheses of the corresponding substituted sodium N-benzyl-D-glucamine-N-carbodithioates, i.e., RC6H4CH2N(CS2Na)CH2(CHOH)4CH2OH (or RBGDTC): CF3BGDTC, R = 3-CF3; MeBGDTC, R = 4-CH3; MeOBGDTC, R = 4-OCH3; ClBGDTC, R = 4-Cl. Examination of the behavior of these compounds as agents for the mobilization of cadmium from its deposits in vivo shows that such substitution can have an appreciable effect on the organ selectivity for cadmium removal, as well as the efficacy. Of the four compounds suggested by the Topliss scheme for pilot studies, two were much more effective than the parent compound (BGDTC), and of these the best one, sodium N-(4-methoxybenzyl)-D-glucamine-N-carbodithioate (MeOBGDTC) reduced kidney cadmium levels to 30% and liver cadmium levels to 50% of those obtained with the parent compound. Sodium N-[3-(trifluoromethyl)benzyl]-D-glucamine-N-carbodithioate (CF3BGDTC) was also found to be superior to the parent compound in reducing the liver cadmium levels, though it was equivalent in reducing renal cadmium levels.

Levels of cadmium in liver and kidneys from Norwegian cervides.

Levels of cadmium in liver and kidneys from Norwegian cervides.

Paradoxical effects of cadmium exposure on antibacterial antibody responses in two fish species: Inhibition in cunners ( Tautogolabrus adspersus) and enhancement in striped bass ( Morone saxatilis)

Previous work in a marine fish, the cunner ( Tautogolabrus adspersus), showed that endocytosis of bacteria by cells in the liver and spleen was affected by 96-h exposure of the fish to cadmium at a concentration of 12 μg/mρ; however, antibody response to sheep erythrocytes was not affected. Since the latter finding was questionable because of short immunization times, and data from more than a single fish species were desirable, both the cunner and an anadromous fish, the striped bass ( Morone saxatilis), were examined for antibody responses against the bacterium Bacillus cereus in Freund's complete adjuvant during a 6- to 8-wk time period. Exposure to 12 μg/mρ cadmium caused significant inhibition of serum antibody titers (P<0.007) in cunners. Paradoxically, antibody response in striped bass exposed to 10 μg/mρ cadmium was enhanced sixfold. This enhancement was weaker but still evident when the antigen was injected 48 d after cadmium exposure. Peritoneal exudate cells from cadmium-exposed striped bass also showed more active migration through microporous filters than cells from non-exposed fish. Since the 96-h cadmium LC 50 was 26 μg/mρ for cunners and 20 μg/mρ for striped bass, the differences in antibody response could not be explained on the basis of differences in cadmium toxicity. Although geometric mean liver cadmium levels after exposure at 15°C were higher in cunners (163.8 ± 1.2μg/gm) than in striped bass (64.4 ± 1.2μg/g), cunners exposed to cadmium at 2°C had lower cadmium levels (46.4 ± 1.2μg/g) which were still effective in inhibiting antibody production at 8°C. On the other hand, striped bass not exposed to cadmium showed a strong, exponential rise in serum antibody when the temperature at immunization, 14°C, was reduced to 9°C; whereas cunners held in the same tanks exhibited a weaker, biphasic serum antibody response. Regardless of the cause (innate differences in cellular response, or better stress adaptability in an anadromous fish), the data show that chemical-stress effects on the immune system of one fish species cannot be extrapolated to another species.

Effect of zinc content in the fodder on cadmium retention in the liver and kidneys in chickens

The influence of zinc content in the diet on cadmium retention in the liver and kidney was studied in growing chicks. Cadmium and zinc concentrations used were similar to those occurring in normally feeds. Increasing cadmium levels in feeds resulted in increasing cadmium concentrations in organs. The cadmium levels in liver were about half as high as in kidneys. Physical conditions and growth rate of the chicks remained unaffected by the cadmium feeding. Cadmium retention in liver and kidneys was related to the zinc content of the diet. Feeding zinc-deficient diets resulted in a 40% higher cadmium retention than feeding adequate zinc diets (50 mg/kg). Excessive zinc levels had no additional effect on cadmium retention.

Long-term sublethal physiological effects on rainbow trout, Salmo gairdneri, during exposure to cadmium and after subsequent recovery

The effects on hematology, blood plasma ion balance and carbohydrate metabolism were studied in rainbow trout, Salmo gairdneri, kept in brackish water, and after 18 and 30 wk of exposure to sublethal levels of cadmium (10 and 100 μg Cd/liter), after 25 and 57 wk of subsequent recovery in clean water. Cadmium exposure caused anemia, hypocalcemia and hypermagnesemia. These effects have previously been observed in fish during shorter periods of exposure, but disappeared in the present study within 25 wk of recovery. This suggests that it is the presence of free cadmium ions during the exposure that is responsible for most of the hematological and biochemical effects. Contrary to these findings, the hyperglycemia, that was present during the exposure, persisted throughout the recovery period. Further evidence for a disturbed carbohydrate metabolism after cessation of the exposure was indicated by a dose-dependent decrease in muscle glycogen content after 25 wk of recovery, and by a dose-dependent increase in liver glycogen content after 57 wk of recovery. These effects on the carbohydrate metabolism could be mediated via the selective uptake of cadmium ions in the pancreas and an inhibitory effect on the insulin secretion, thus resulting in an abnormal endocrine control of the metabolism. Analyses of cadmium revealed a dose-dependent accumulation of cadmium in the muscle, that had reached equilibrium after 18 wk of exposure, while a continuing and dose-dependent accumulation was found in the liver throughout the exposure. The levels attained in the liver were about 100 times higher than in the muscle. The slow elimination from liver and kidney suggest a biological half-life for cadmium of more than 1 yr in these tissues. A close positive correlation was found between cadmium levels in liver and in kidney ( r = 0.90) in fish after 57 wk of recovery.

Heavy Metals in Tissues and Organs of the Narwhal (Monodon monoceros)

Sixty narwhals (Monodon monoceros) were sampled in the vicinity of Pond Inlet, N.W.T., during the summer of 1978 and 1979. Concentrations of Pb, Cu, Cd, Hg, Se, As, and Zn were measured in liver, kidney, muscle, and blubber. All elements except As were lowest in concentration in the blubber and highest in kidney and liver; Cd and Zn were generally higher in kidney than in liver, while the converse was true for Pb, Cu, and Hg. Cadmium levels in liver and kidney had high interanimal variability but were generally higher than would be expected based on existing marine mammal Cd content data. Certain metals were correlated with animal size and sex. Mercury in kidney, muscle, and blubber and Cd in muscle appear to accumulate during growth. A number of interelement associations with extremely high probabilities were found: among them, that between Hg and Se and Cd and Zn in liver and kidney tissues. The latter association is thought to be related to the presence of metallothionein. The data presented are discussed in terms of what little is known of the biology of the narwhal and represent a baseline for a resource organism which is harvested in an area already subject to mining activity, the level of which is due to increase in the future.

Trace metals in the common porpoise, Phocoena phocoena

Mercury, cadmium, copper and zinc concentrations in brain, liver, kidney, heart and spleen of twenty-six specimens of the common porpoise ( Phocoena phocoena) are presented. Mercury and cadmium levels in liver and kidneys tended to increase with length of animal, but copper and zinc levels show no such trends. The proportion of methylmercury to total mercury (9%–57%) in the liver decreased with increasing total mercury concentrations. Lead, chromium, nickel and cobalt levels were below the analytical detection limits (0·5, 1·0, 1·0 and 2·5 μg −1, respectively).

Liver cadmium levels in North Carolina residents who died of heart disease.

Using data from an autopsy series, we found a strong positive correlation between liver concentration of cadmium and death from heart disease. In view of recent experiments reported in the literature, the possibility that a low level of cadmium has a toxic effect on the cardiac conduction system is supported.

Cadmium levels in hair and other tissues during continuous cadmium intake

Rats that received cadmium 300 ppm in drinking water (average daily cadmium intake = 4.5 mg/rat) for 12 wk attained peak cadmium levels of 112, 34, and 19 mug/g in hair, liver, and kidney, respectively, at week 4. Rats that ingested cadmium 200 ppm (average daily cadmium intake = 3.6 mg/rat) for 13 wk attained peak cadmium levels of 29 mug/g in kidney at week 7, and 94 and 27 mug/g in hair and liver, respectively, at week 9. Despite continuous exposure to the heavy metal, tissue cadmium concentrations declined to steady-state levels of 24-33 mug/g in hair and 10-17 mug/g in liver and kidney. Histopathologic effects were not observed in liver or kidney. In contrast to cadmium in hair, blood cadmium levels, which remained consistently low (less than 0.04 mug/ml) throughout the study, did not correlate with changes in cadmium levels in liver and kidney.