This study explores the enhancement of catalytic efficiency and 1-ethyl-3-methylimidazolium acetate ([Emim][OAc]) tolerance in the single-step saccharification of mixed cabbage residue (MCR) biomass using entrapped cellulase. Entrapping cellulase in calcium alginate hydrogel beads achieved an entrapment efficiency of 75.90 ± 1.83 %. In hydrolysis systems with 2 % and 10 % [Emim][OAc], the entrapped cellulase demonstrated 5.3 % and 6.5 % higher activity than free cellulase, respectively. After five cycles, the entrapped cellulase retained 93 % and 87 % of its initial activity in 0 % and 2 % [Emim][OAc] hydrolysis systems, respectively. Remarkably, cumulative glucose yields with entrapped cellulase were 3.51 and 3.10 times greater than those with free cellulase in 0 % and 2 % [Emim][OAc] hydrolysis systems, respectively. These results highlight the superior performance of entrapped cellulase in maintaining enzyme activity and improving glucose yields. Notably, while [Emim][OAc] is effective in separate pretreatment stages, its addition is unnecessary for the single-step saccharification of MCR biomass. This study underscores the promising potential of entrapped cellulase as a catalyst in enhancing the efficiency of biomass saccharification processes.
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