Burnt Bones Research Articles

An innovative way to use 3D modeling on burnt bone to differentiate heat fractures from blunt and sharp force trauma

AbstractAssessments of blunt and sharp force trauma in forensic research are frequently reliant on research with individual long bones. As a result, information on the interpretation of the trauma on irregular bones is limited in unburned bones and an even bigger discrepancy is found if the fracture relates to blunt/sharp force in cremated bone. This research strives to differentiate between traumatic fractures and heat fractures in flat and irregular bones. Five human calottes and five human hemipelves were exposed to either blunt or sharp force trauma and then all were incompletely cremated. One hundred and eighty fractures, representing a mixture of traumatic and heat fractures, were captured using a Keyence VHX‐2000 digital microscope and analysis was done in combination with 3D software, Geomagic Studio 2014 and Geomagic Design X (2016). With virtual reconstructions and reverse engineering facilitated by the software, we were able to discern fracture boundaries, slopes, and variances between fracture types. 3D representation provided the ability to differentiate peri‐mortem trauma from heat fractures based on curvature analysis of fracture walls. Evidence of trauma types (blunt versus sharp) were found to be distinguishable at their impact site based on this curvature examination; however, shallow, secondary or tertiary trauma fractures were difficult to discern from heat fractures. Blunt force trauma impact sites and sharp force trauma impact sites were easily identifiable; secondary trauma fractures were sometimes clearly noted but may be misinterpreted. Overall, deep trauma fractures and heat fractures can be discerned from one another using this technology.This article is categorized under: Forensic Anthropology > Taphonomic Changes and the Environment Forensic Anthropology > Trauma Analysis Forensic Chemistry and Trace Evidence > Fire Debris Analysis

Cooked or discarded? Experimental distinction of rabbit burnt bones and its application to the archaeological record

Leporid bones from Middle Palaeolithic assemblages may result from the contributions of various predators, including humans, or natural processes. Although the acquisition, processing, and consumption of small mammals by Neanderthal groups has been widely demonstrated, there are still some unresolved issues. Cut marks are typically the most reliable indicators of human processing of carcasses. However, animals the size of rabbits often pose a challenge in this regard, as the use of stone tools is not always necessary for their consumption, particularly for meat or marrow extraction. Consequently, the quantity of these types of marks, such as cuts or hammer-stone percussion damage, indicating human processing, may be limited. Burning is another type of bone damage that could be indicative of human action, although both intentional and accidental processes could lead to the thermal alteration of remains without necessarily linking them to consumption. Therefore, efforts to distinguish the processes resulting in bone burning are of vital importance in determining the origin of these animals in archaeological assemblages. In this work, the results of several experimental series designed to characterise the roasting and subsequent cleaning of waste on rabbit bones are presented. These results confirm most of the characteristics described in previous experimental works on burnt bones, highlighting the differential damage between bones with and without meat. The current study aims to contribute new data for characterising burned rabbit bones resulting from human actions, which can then be applied to Middle Palaeolithic assemblages with this type of thermal alteration bone modifications.

A Study of Species and Individual Differentiation of Burnt and Unburnt Bones Using X-Ray Fluorescence

Commingled remains analysis is a fundamental problem in bioarchaeology and forensic anthropology. In cases of commingling, the bones of all individuals represented must be accurately and reliably differentiated. X-ray fluorescence spectrometry (XRF) can identify species and individuals when bone fragments are found in archaeological or forensic contexts. The present study aimed to verify whether portable X-ray fluorescence spectrometry (pXRF) can be used to differentiate bone fragments at the species level (Bos taurus, Sus domesticus, Equus caballus, and Homo sapiens) and at the individual level of human individuals. We also aimed to verify whether species and individuals can be differentiated using pXRF even if the bones were burned. A total of 119 adult human bones from archaeological sites in Central Moravia and Silesia and 17 adult non-human bones from archaeological sites in Central Moravia (Czech Republic) were examined. All bones are dated to various periods (from the Bronze Age to Modern Times). When differentiating the unburnt and burnt bones at the species level, the overall accuracy of classification was 84.6% and 93.9%, respectively. When differentiating unburnt human bones at the individual level, the correct classification ranged from 88.1% to 72.7%. The differentiation of the burnt bones of humans at the individual level achieved an average success rate of more than 60%. The results confirmed that pXRF can be used for species and individual differentiation of unburnt bones and is almost equally applicable to burnt bones.

The ecology, subsistence and diet of ~45,000-year-old Homo sapiens at Ilsenhöhle in Ranis, Germany

Recent excavations at Ranis (Germany) identified an early dispersal of Homo sapiens into the higher latitudes of Europe by 45,000 years ago. Here we integrate results from zooarchaeology, palaeoproteomics, sediment DNA and stable isotopes to characterize the ecology, subsistence and diet of these early H. sapiens. We assessed all bone remains (n = 1,754) from the 2016–2022 excavations through morphology (n = 1,218) or palaeoproteomics (zooarchaeology by mass spectrometry (n = 536) and species by proteome investigation (n = 212)). Dominant taxa include reindeer, cave bear, woolly rhinoceros and horse, indicating cold climatic conditions. Numerous carnivore modifications, alongside sparse cut-marked and burnt bones, illustrate a predominant use of the site by hibernating cave bears and denning hyaenas, coupled with a fluctuating human presence. Faunal diversity and high carnivore input were further supported by ancient mammalian DNA recovered from 26 sediment samples. Bulk collagen carbon and nitrogen stable isotope data from 52 animal and 10 human remains confirm a cold steppe/tundra setting and indicate a homogenous human diet based on large terrestrial mammals. This lower-density archaeological signature matches other Lincombian–Ranisian–Jerzmanowician sites and is best explained by expedient visits of short duration by small, mobile groups of pioneer H. sapiens.

Burning temperature and bone modification: The cremation dynamics

Burning a body is one of the best-known methods to conceal a crime. The aim of this study is to identify thermal changes in bones burned at pre-set temperatures. 100 cadavers undergone cremation were analysed. Cremation temperatures ranged from 600 to 1200 °C, whereas cremation time was monitored. Morphological and colour changes of the bones, the development of fire-related fractures and surface were studied to analyse bone response to thermal alteration.

Formation processes, fire use, and patterns of human occupation across the Middle Palaeolithic (MIS 5a-5b) of Gruta da Oliveira (Almonda karst system, Torres Novas, Portugal).

Gruta da Oliveira features a c. 13 m-thick infilling that includes a c. 6.5 m-thick archaeological deposit (the "Middle Palaeolithic sequence" complex), which Bayesian modelling of available dating results places in MIS 5a (layers 7-14) and MIS 5b (layers 15-25), c. 71,000-93,000 years ago. The accumulation primarily consists of sediment washed in from the slope through gravitational processes and surface dynamics. The coarse fraction derives from weathering of the cave's limestone bedrock. Tectonic activity and structural instability caused the erosional retreat of the scarp face, explaining the large, roof-collapsed rock masses found through the stratification. The changes in deposition and diagenesis observed across the archaeological sequence are minor and primarily controlled by local factors and the impact of humans and other biological agents. Pulses of stadial accumulation-reflected in the composition of the assemblages of hunted ungulates, mostly open-country and rocky terrain taxa (rhino, horse, ibex)-alternate with interstadial hiatuses-during which carbonate crusts and flowstone formed. Humans were active at the cave throughout, but occupation was intermittent, which allowed for limited usage by carnivores when people visited less frequently. During the accumulation of layers 15-25 (c. 85,000-93,000 years ago), the carnivore guild was dominated by wolf and lion, while brown bear and lynx predominate in layers 7-14 (c. 71,000-78,000 years ago). In the excavated areas, conditions for residential use were optimal during the accumulation of layers 20-22 (c. 90,000-92,000 years ago) and 14 (c. 76,000-78,000 years ago), which yielded dense, hearth-focused scatters of stone tools and burnt bones. The latter are ubiquitous, adding to the growing body of evidence that Middle Palaeolithic Neandertals used fire in regular, consistent manner. The patterns of site usage revealed at Gruta da Oliveira are no different from those observed 50,000 years later in comparable early Upper Palaeolithic and Solutrean cave sites of central Portugal.

Determining the Postmortem Timing of Sharp Force Damage and the Pre-burning Condition of Burnt Bone

Determining the Postmortem Timing of Sharp Force Damage and the Pre-burning Condition of Burnt Bone

Analysing criteria of formation in multi-stratified karst deposits: the observation of surfaces in Late Pleistocene faunal bones from Mora Cavorso cave (Latium, Central Italy)

ABSTRACT Mora Cavorso cave is mostly known for preserving one of the largest human burials of early Neolithic in Central Italy, but it holds archaeological and palaeontological finds ranging from Late Pleistocene to historical times. The observation of faunal bone surfaces from Late Pleistocene layers has been carried out mainly to verify the human contribution to the formation of the bone accumulations. The assemblages are made up of about 3750 finds, 48% of which taxonomically identified, while 31% of the total show traces of surface alterations. Layer 8, the deeper and partially excavated, shows few traces on 28 finds with a dark patina. For Layer 7, boluses by birds of prey, traces of digestion and gnawing by small carnivores show the main agents of the formation of the bone accumulation. For Layer 6, just a small percentage of finds shows traces referrable mostly to small carnivores, with a few traces referrable to rodents. For Layer 5, gnawing of small carnivores was observed, however the occurrence of burnt bones and cut traces confirmed the hypothesis, based on the discovery of lithic artefacts, that the oldest human attendance in the cave is here attested and dates to Epigravettian period.

WOODEN CONSTRUCTIONS IN THE BARROWS OF THE RAIKY CULTURE: GENESIS, ANALOGIES, SEMANTICS

Wooden constructions of various types were found in the barrow cremation burials of the Raiky culture of the 8th—10th centuries. People of this culture were the Slavic tribes of the Right Bank of the Dnieper, Volynia, and Dniester River regions. The burnt rectangular constructions made of horizontal logs (Mezhyrichky, Velyka Horbasha and Radastst in Polesie) were the most widespread. Burial chambers from the Don River basin (Borshevo culture) or Transylvania are usually cited as parallels. However, in terms of typology, wooden constructions spread over the territory of Poland, the Czech Republic, Slovakia and East Germany are much closer. They are considered as models of residential buildings — «houses of the dead».
 Circular fences made of burnt pillars are less common (Holovne and Mylanovichi in Volynia). It is believed that they were supposed to separate the World of the Living from the World of the Dead.
 Most of the barrows from the Chornivka (Bukovina) and Dobrostany (Roztocze) cemeteries had the pillar holes on the periphery. This element which has usually been overlooked by researchers is also widely known in West Slavic lands: modern Czech archaeologists even consider the burials of this type to be dominated in the territory of Bohemia. It is believed that burial urns were placed on the pillars because around them on the surface of the mound the burnt bones and fragments of ceramics were often recorded. Thus, the words of the Old Rus chronicler who described the burial of pagan Slavs «on a pillar by the roads» should be understood quite literally. The recording of such burials during archaeological excavations causes understandable difficulties.
 In general, in the area of the Raiky culture, there can be counted at least one and a half dozen mound burials with the remains of wooden structures. The homogeneity of constructions demonstrates the common worldviews that were widespread in the 8th—10th centuries among the Slavs from the Elba to the Dnieper.

Detection of methamphetamine in mouse femurs exposed to high temperature.

Bone samples are valuable for examining the cause of death and circumstance leading up to death when body fluids are not available for forensic toxicological analysis. Examined were heat-induced changes in methamphetamine and amphetamine concentrations in femurs removed from methamphetamine-injected mice to determine if the burned bones could be used for toxicology testing. The femurs were heated at 100°C, 300°C, or 500°C for 10 or 30 min. The tissue structure of the heated femurs was preserved at 100°C for 30 min but was destructed at higher temperatures. Methamphetamine and amphetamine were detected in femurs heated at 100°C for 10 min, 100°C for 30 min, and 300°C for 10 min (with methamphetamine and amphetamine concentrations ranging from 0.36 to 35 μg/g and 0.54 to 47 μg/g, respectively). Methamphetamine and amphetamine were detectable when heated above their decomposition temperature as a result of limited heat transfer do to protection provide by the femoral muscle. Thus, the bone could be a useful analytical sample in cases of burn-related deaths, where it is difficult to collect body fluids.

Red and Black Paints on Prehistoric Pottery of the Southern Russian Far East: An Archaeometric Study

This paper considers the results of an examination of painted pottery from prehistoric sites of the Prmor’ye region (Southern Russian Far East) in the northwestern part of the Sea of Japan basin. Red-painted and black-painted ceramic wares occur here only in the remains of the Yankovskaya archaeological culture dated to the 1st mil. BCE. Red painting appears as a colored surface coating, and black painting is represented by very simple drawn patterns. Until recently painting decorations have not been intentionally studied. The objects of our investigation are a small series of red-painted and black-painted ceramic fragments originated from archaeological sites. The methods of optical microscopy, SEM-EDS, and Raman spectroscopy were applied to the study of research materials. As a result, the data on characteristics of texture and composition of red and black paints were obtained. Both were determined to be pre-firing paints. Red paint is a clayish substance mixed with natural ochre pigment containing the hematite coloring agent. Black paint is carbon-based. Black carbon and burnt bone are recognized as colorants. The presented materials are new evidence of pottery paint technologies in prehistoric Eurasia.

Development of a Colorimetric Scale as an Aid for Estimating Temperature of Burnt Bone

Background: Forensic pathologists, archeologists, and forensic anthropologists are often required to interpret burnt bones. One of the key factors for an accurate interpretation of the remains and the events leading to incineration is the estimation of the maximum exposure temperature of the human remains. Several types of research focus on temperature estimation, using color assessment, histology observations, and mechanical changes, which can help in the most accurate interpretation. The color of burnt bone is inevitably related to the time and temperature(s) to which it was exposed, the size and condition of the bone, and the environment. Nevertheless, it can be possible to estimate exposure temperature based on visual observation and analysis. Objective: Development of a colorimetric scale for photographic documentation and the color calibration of burnt bone. Method: This paper proposes a patented prototype scale with calibrated colors of burnt bone colors at different temperatures, based on the existing RGB color published data as a visual aid in the interpretation and estimation of the maximum temperature exposure of burnt bone. Results and Discussion: Authors are aware that the estimation of the maximum temperature of burnt bone cannot be determined solely by colors. Visual color recognition is prone to errors. Conclusion: The proposed device should be considered a tool during photography of burnt bone and aid in interpreting temperature estimates.

Survival of sharp force trauma in burnt bones: effects of environmental factors

This study investigates how environmental variables, such as temperature and rainfall, affect previously induced cut marks on burnt bones. This research used non-serrated and serrated blade knives to inflict trauma on Sus scrofa ribs (n = 240). The bones were later burnt and left for 1 month in a taphonomic experimental facility. Qualitative and quantitative examinations were conducted using macroscopic and microscopic techniques to assess specific characteristics of the cut marks. Any changes to the dimension and morphology of the cut marks as well as their level of fragmentation were recorded.This study has led to three important outcomes: (1) identification of pre-existing cut marks is possible in reconstructed burnt bone fragments; (2) cut marks from different types of knife blades showed dissimilar responses to heat and the environment; and (3) specific environmental variables affect burnt bone fragmentation. These results have implications for trauma analysis on burnt remains in forensic anthropology casework.

Reaching new heights: Testing the performance of metric approaches to estimate stature from burned skeletal remains.

Bone heat-induced changes complicate osteometric stature estimation of human remains from forensic settings. The validity of current methods has not been tested to a great extent. Our aim was to determine how precise are stature estimations obtained from three different approaches, namely by using (i) Rösing's method (Rösing 1977), (ii) a 10% shrinkage correction factor (Strzałko et al. 1972) and (iii) chemosteometry (Gonçalves et al. 2020). For this purpose, pre- and post-burned head measurements from the humerus, radius and femur were used. The sample comprised 46 human skeletons of known sex and age-at-death. These were experimentally burnt to maximum temperatures ranging from 700 to 1100°C (attained after 90-188 min) for other research purposes. Stature estimations were performed through measurements in both pre-burned and burned bones using the three approaches and based on the method of Olivier and Tissier (1975). Mean absolute differences and the relative technical errors of measurements (TEM%) between the pre-burned and the estimations were calculated. Absolute mean differences indicated that the chemosteometric approach allowed for potentially more precise stature estimations than the other two procedures. However, the TEM% was very low for all approaches (smaller or close to 1%), and stature estimations were thus well within the error margin reported by Olivier and Tissier (1975). Results suggest that the chemosteometric approach enables more accurate predictions of the actual heat-induced shrinkage of each bone thus rendering more precise stature estimations. Nonetheless, the other procedures also provided quite reliable estimations although they require confirmation that the bone is calcined.

A micro-geoarchaeological view on stratigraphy and site formation processes in the Middle, Upper and Epi-Paleolithic layers of Sefunim Cave, Mt. Carmel, Israel

This paper presents a micro-geoarchaeological study carried out on the sedimentary sequence exposed at the entrance of Sefunim Cave, Israel, a sequence that spans from the Middle Paleolithic to the early Epipaleolithic periods. Using FTIR and micromorphological techniques, we investigated the stratigraphic sequence to reconstruct patterns of site use and archaeological formation processes. We identified formation processes that are common among Paleolithic caves sites in the Southern Levant, mainly the deposition of local terra rossa through colluvial sedimentation. Taphonomic disturbances of the deposits range from minimal to moderate, exhibited mainly by root and burrowing activity, but with no evidence for significant transport of archaeological materials. While the upper layers (II–III) are decalcified, the precipitation of secondary calcite results in increasing cementation of the sediments with depth in the lower layers (V–VII). We observed variation at the microscopic scale and identified an inverse correlation between human and carnivore activity throughout the layers. We observed human activity by the presence of micro-archaeological materials such as chert, bone, charcoal, rubified clay, burnt bone and shell, and wood ash. We observed carnivore activity by the presence of phosphatic grains and coprolite fragments as well as chewed and digested bones. We conclude that human activity at the site was characterized by episodes of varying intensity, based on the frequency of archaeological finds within the different layers. The alternating episodes of human and carnivore activity at Sefunim Cave may demonstrate the close-knit interactions and reciprocal relations that humans and carnivore shared at Paleolithic caves.

Faunal Remains Associated with Human Cremations: The Chalcolithic Pits 16 and 40 from the Perdigões Ditched Enclosures (Reguengos de Monsaraz, Portugal)

Abstract Different funerary behaviors are recorded in the Iberian Peninsula during Late Prehistory. Cremation is not the most common practice and the association between human cremains and fauna is even scarcer. We present two Chalcolithic pits (pits 16 and 40) from the Perdigões ditched enclosures, Reguengos de Monsaraz, Portugal. Humans were accompanied by animals and other votive materials such as arrowheads, ivory anthropomorphic figurines, and marble idols. Differences between the two contexts are discussed regarding the selection of faunal anatomical parts, the abundance of species, and the manipulation of remains. The results obtained were compared to previously published data from anthropological analysis. Burning damage intensity is different among pits and between humans and fauna. Hence, this suggests that the latter also resulted from diverse practices, including the possible selection of animal body portions for cremation and/or the deposition of selected burned bones or even related to patterns existing in the contexts of the provenance of the cremated materials before the cremation events.

DNA extraction of burnt bone and teeth casework samples using bead-beating homogenization technique

Sample disruption was a necessary step for DNA isolation. Bone and teeth were useful biological sources particular in human remains and advance decomposed bodies. The compact bone and teeth required several preparation steps prior to analyzing process. However, the methods in standard protocol were laborious and time consuming. An alternating pulverization, bead beating homogenizer, was purposed in its effectiveness for forensic casework. (1) Here, we applied this technique to the burnt cracked bone and tooth that recovered from house fire for forensic DNA analysis. After cleansed an external surface, the eight multidirectional motion tissue homogenizer, Precellys® evolution, was utilized to pulverize bone and tooth followed by a DNA extraction and amplification. For detection with a capillary electrophoresis, full profiles of autosomal STRs and Y-chromosomal STRs were recovered from tooth sample but the partial profile STR was demonstrated in bone sample. The new technique in bone homogenization was less time consuming (around 30 s), less exposure to chemical agents (no need of liquid nitrogen), high efficiency, with high-throughput productivity.

Analysis of an ancestry using cremated old human remains from the Korean War victims

Analysis of DNA from burnt bone fragment is the very hard work for the human identification in forensic casework. In general, cremated bone with an artificial damage is more difficult to get an intact DNA than a singed one because of the chemical and biological drastic changes such as protein denaturation and destruction. In this study, we pursue the best technical approach for the minimal damage and the contamination of DNA from other factors in the preconditioning and the extraction process based on over 70 years old Korean War victim skeletal that was burnt and buried in Korean Peninsula. First of all, we removed the pollutant and the dust from the burnt bones using dental instruments, and then incubated with EDTA buffer at 25 ℃ to remove inhibitors such as calcium and mineral. In order to compare the DNA preservation ability between a pellet and a supernatant, samples are repeatedly tested to collect washed EDTA buffer several times to separate. Each of isolated materials is secondly cleaned with the organic extraction method using phenol and analyzed mtDNA sequence with the in-house method for the ancestry assay. The better discrimination ability was appeared in the supernatant than the pellet. Nevertheless, many of the forensic geneticists use a powdering method for getting more DNA, we applied EDTA buffer in the preconditioning step to eliminate every contamination. As a result, the contamination factor was efficiently removed and the ancestry was estimated as per the written information. Consequently, cremated bone is identified to belong in the D4 mtDNA haplogroup which is commonly reported in ethnic groups in Asia especially Korea. This is a preliminary study of a human identification over an ancestry analysis to give information against a mass disaster in a future. Through a higher process optimization and better analytical methods toward more remains, which are genetically difficult to analyze, will support to examine the identity of the post cremated remains.

Let's make a mess, maybe no one will notice. The impact of bioturbation activity on the urn fill condition.

The research was carried out at the cremation cemetery of the Lusatian culture in Wtórek, Ostrów Wielkopolski district, Wielkopolska province, Poland. Contrary to the so-far-studied topics related to the CT imaging of burnt bones and their virtual exploration, we concentrated on the analysis of the structures formed by the soil fauna activity in the fills of urns and additional vessels, and reconstruction of the dynamics of the ecosystem variability within the cemetery area based on thereof. We also demonstrated the impact of macrofaunal activity on stratigraphy and bone fragmentation. From the total of 222 excavated burials in 18 urns and one additional vessel, the remains of macrofauna or its bioturbation activity were identified. Out of 19 vessels subjected to CT examinations, traces of macrofaunal activity were demonstrated in 13: in five vessels animal bioturbative activity was not observed and in one, observations was impossible (due to significant metal-related artifacts). In two vessels both macrofaunal remains and traces of activity were identified. Discovered bioturbations were associated with specific species or genera. Nests or their parts of the genus Geotrupes sp. beetles were the most frequently observed traces of macrofaunal activity. Tunnels and aestivation chambers of earthworms and chambers of the genus Harpalus sp. beetles filled with Setaria sp. caryopses were discovered. The chitinous parts of other insects and the humerus bones of the vole of the genus Microtus sp. were also identified. It was shown, especially due to the non-destructive method, that rodents activity had the most destructive effect on the bone stratigraphy as well as on the movement and fragmentation of the burnt bones. The chances of visualizing bioturbations decreased with time since their creation. The process of disappearance of traces of macrofaunal activity concerned both traces of rodent activity and nests set up by Geotrupes sp. and other species.

FTIR study on the phase transition of experimental and archaeological burnt ivory

Recent excavation of the elephant ivories at the Sanxingdui site of the Shang Dynasty in Sichuan, China brings attention to the burnt features of the ivories. However, burnt ivories cannot be assessed by the same criteria established for burnt bones because of the differences in the ways that the two materials respond to heat. This study examines differences in the phase transition characteristics of ivory and bone, and confirms that the threshold temperature of the alteration in ivories is lower than that in bones. In our analysis, elephant ivories and bovine bones burnt at the temperatures between 200 and 1000 °C in oxidizing or reducing atmosphere are prepared and comparatively investigated by attenuated total reflection Fourier transform infrared spectroscopy. The results show that the transformation from magnesium-substituted hydroxyapatite (Mg-HAp) to magnesium-substituted β-tricalcium phosphate in ivory dentin takes place at the temperature as low as 800 °C, while β-tricalcium phosphate is not observed in bovine bones burnt at 1000 °C or below mainly because of the different magnesium contents. Due to the destruction of Mg-HAp at 800 °C in ivories, cyanamidapatite that could form in bones burnt under reducing atmosphere is absent in burnt ivories. With reference to the experimental burnt ivories, archaeological burnt ivories from the Sanxingdui site can now be clearly determined to be burnt unevenly at temperatures up to around 800 °C. This study may provide further information for archaeological research on ivories from the Sanxingdui site and identification of burnt bioapatite materials in other archaeological findings.