Treatment Of Burns Research Articles

PSYCHOLOGICAL DISORDERS IN BURN PATIENTS: A META-ANALYSIS OF DISORDERS AND TREATMENTS

The main subject of this paper is the psychological disorders experienced by burn patients, with a particular emphasis on the necessity of psychological intervention in their treatment. The research seeks to investigate prevalent mental health conditions, including post-traumatic stress disorder, depression, and anxiety, and assess the efficacy of various therapeutic approaches.The method relied on a comprehensive review of prior literature and a thorough evaluation of the outcomes from relevant studies. The findings indicated that a significant portion of burn victims experience various psychological disorders. This includes post-traumatic stress disorder (PTSD), depression impacting, and anxiety affecting. The research also highlighted the significance of providing psychosocial support to enhance treatment results, as well as the effectiveness of Eye Movement Desensitization and Reprocessing (EMDR), Cognitive-Behavioral Therapy (CBT) and Virtual Reality (VR) techniques in addressing these conditions.To ensure comprehensive and effective patient care, it is recommended to integrate psychological interventions into burn treatment protocols, as stated in the paper's conclusion.

Effects of burning on vegetation, soil physicochemistry and prokaryotic microbial communities in surface and subsurface peat

Prescribed burning is a common management strategy in peatlands that has the potential to affect soil physicochemistry, alter biogeochemical cycles and trigger changes in vegetation structure. How burning affects prokaryotic community composition across different soil profiles is not well understood. This study explored the effects of prescribed burning on the diversity of prokaryotic communities in peat soils. Soil samples were collected from Moor House Nature Reserve, UK, a long-term monitoring site initiated in 1954 subject to three burning treatments: Burning at short rotations every 10 years, burning at long rotations every 20 years and a non-burn control. Observed species richness for archaea was highest in the topsoil of the non-burn control plots and highest for bacteria in the topsoil of the non-burn control and plots under a long rotation regime. Community composition was significantly different between different burn treatments and soil depth. Archaeal community structure was shaped by NH4+ and pH in the topsoil; by Pb, moisture and Al in the 20–40 cm profile; and by total N, total C, Al, Ca, Fe and pH in the 40–60 cm profile. Bacterial community structure was shaped by NH4+, heather cover, pH and Mg in the topsoil; by Fe, K and Pb in the 20–40 cm profile; and by Al, Ca and Fe in the 40–60 cm profile. A co-occurrence network analysis revealed that the topsoil of the non-burn control plots had a more complex network structure with more positive links than those under a rotational burn, but a higher average connectivity with a higher number of negative links was observed in the long rotation 20–40 cm profile. The results provide a new insight into the response processes of soil prokaryotic communities to burning in peatland soils, providing valuable knowledge that can support the evaluation and management of ecosystem services in peatlands.

Cubosomes hydrogel containing silver based organometallic compounds for enhanced wound healing of burns: In vitro and in vivo studies

The aim of this study is to investigate the treatment of burn wounds and bacterial infections, specifically focusing on silver(I) N-heterocyclic carbene (Ag-NHC) loaded into cubosomes. Ag-NHC is an organometallic complex of silver (Ag) and can be considered an active pharmaceutical ingredient for the topical treatment of burns. A novel nanoparticle system, cubosome dispersions, was formulated using high pressure homogenization methods with different concentrations of lipid phase glyceryl monooleate (GMO), surfactants such as poloxamer 407 (F-127), and polyvinyl alcohol (PVA). The optimized formulation (U6) was characterized by in vitro drug release profile (90.54 ± 1.17 %), particle size (126.7 ± 0.98 nm), zeta potential (−21.6 ± 6.83 mV), entrapment efficiency (89.30 ± 1.46 %), and morphology as observed by transmission electron microscopy (TEM). The U6 formulation was incorporated into a hydrogel containing Carbopol 940 to form a cubosomal hydrogel (cubogel). The cubogel was characterized by the in vitro release of Ag-NHC (92 %), entrapment efficiency (>90 %), pH (5.3), viscosity (1.2 cP), spreadability, and TEM. From the in vitro drug release pattern, it was concluded that the U6 formulation showed slow and sustained drug release over 24 h, and demonstrated superior burn healing compared to marketed products. The in vivo wound healing study revealed that the cubogel demonstrated a superior burn healing rate compared to commercially available products (Quench® and Clotrimazole®). The in vivo histopathological results showed that the prepared cubogel was effective in treating second-degree burns, with no side effects or skin irritation, compared to commercial products. Additionally, Ag-NHC-loaded cubogel facilitated sustained release for treating burn wounds without any bacterial infections. The current findings provide a strong basis for the initiating phase 0 clinal trials, highlighting significant potential for the further research to benefit the pharmaceutical industry.

Survey about target temperature and thermal management in intensive care for severe thermal trauma in burn centres of Germany, Austria and Switzerland

Burn trauma induces hypermetabolism and alters thermoregulation resulting in elevated body temperature. Because patients with burns are prone to heat loss and hypothermia, maintaining physiologic body temperature is important. However, optimal target temperature is widely unknown because thermoregulation of burn trauma has mainly been studied in the last century when treatment concepts differed from current one. The aim of this study was to investigate current thermal management of burn treatment and to investigate the discrepancies between classical concepts of thermoregulation in burn trauma and current practice of temperature management.A paper-based survey was conducted in burn centres in Germany, Austria and German-speaking Switzerland. Participants were asked for expected temperatures, temperature goals and thermal management of severely burned patients. Results were evaluated for adults and children.37 of 38 approached burn centres participated in this survey. 59% expected that adults with burn trauma would develop hyperthermia (>37.5°C) but only 27% expected hyperthermia in children (>38°C). The average target body temperature was 37.1°C for adults and 36.9°C for children. Adults below 35.7°C and children below 36.0°C were supposed to be hypothermic. Temperatures above 38.8°C in adults and 38.7°C in children raised suspicion for sepsis. Antipyretic treatment was assumed to be justified at temperatures above 39.1°C in adults and 38.5°C in children. Although the most common warmth protective method was to increase ambient temperature, 89% of all participants felt their wellbeing was affected by an increased ambient temperature and 68% were concerned about temperature related negative effects. Although 57% of the responding centres had established a standard operating procedure for thermal management, only 41% considered the available literature to be very relevant in daily practice and 89% criticized the lack of guidelines.Limit- and target-temperatures in European burn centres are heterogeneous. Classic concepts of thermal management in burn care are not generally adopted. A majority of the centers expresses the need for specific guidelines. The basis for this should be multicentre clinical trials on temperature management in burn trauma.

Fresh tilapia fish skin graft treatment of severe skin burns in a cat

AbstractAn entire female cat presented with extensive third‐degree burns covering greater than 30% of total body surface. The wound was cleaned and debrided with daily dressing management to heal with second intention. Dressing applied included honey‐based product, L‐Mesitran Ointment, and silver alginate dressing, Askina Calgitrol Thin, until healthy granulation tissue had formed. Fresh tilapia skin grafts were harvested and prepared and secured onto the wound bed. Daily lavage and graft evaluation occurred, and subsequently the skin grafts were removed on Day 13 and Day 7, respectively. Wound healing was observed without any complications or adverse side effects. In conclusion, the use of fresh tilapia skin grafts proves to be a safe, innovative, low‐cost, and effective therapeutic and promising option in treatment of challenging skin burn wounds. Tilapia fish skin grafts resulted in a clinically favourable outcome in a cat with extensive third‐degree burn wounds.

Silver loaded functionalized calix [4]arene nanofibers and evaluation in burn wound healing

Burn treatments with gene therapy and healing factors requires highly cost techniques. Instead, topical formulations including plant extract or pharmaceuticals are offered for wound-healing effect. However, these have low water solubility and cannot uptake into cells sufficiently. To overcome disadvantages, new effective treatment approaches should have been accessible. This study aimed to develop nanofiber-based wound coverings using four different Ag(I)-calixarene derivatives. Their nanofibers were produced by the electrospin method, and evaluated in vivo in rats with a second-degree burn model. Biochemical analysis has been maintained to exmine the effects of formulations on such as growth factors and some cytokines that are effective in wound healing process. In addition, histopathological analyzes has been performed to evaluate the presence and density of granulation tissue, inflammation, epithelial thickness and fibrosis. All data were compared with a commercial topical, silver sulfadiazine, whose usage is limited due to various disadvantages in the treatment processes of burn wounds. In this study, four different calixarene derivatives were synthesized their nanofibers were produced by the electrospin method, and their effectiveness was evaluated in vivo in rats with a second-degree burn model. Then, various biochemical and histopathological analyses were done to elucidate the possible wound-healing mechanism. When considering both pathological and biochemical data, the nanofibers of CLX-SAMD-Ag (5,11,17,23‐tetra‐tert‐butyl‐25,27‐bis(Sulfadiazine)‐26,28‐propylcalix (Xie and Unser, 2013) [4]arene - Ag) and CLX-MORF- Ag (5,11,17,23‐tetra‐tert‐butyl‐25,27‐bis(3-Morpholinopropil)‐26,28‐dihydroxycalix (Xie and Unser, 2013) [4]arene-Ag) were found to be more effective than silver sulfadiazine -silver content application, also known as traditional burn treatment for wound healing.

Understanding Burn Management For Nursing Students

Background: Managing burn injuries, particularly in mass-burn scenarios, requires immediate first aid and early care, which are critical for improving outcomes. The quality of burn treatment is highly dependent on the knowledge and practical skills of nursing students, particularly those assigned to burn units. Knowledge Gap: Despite the critical nature of burn care, limited research has been conducted on the level of knowledge nursing students possess in this area, especially in regions like Basra, Iraq. Aims: This study aimed to assess the knowledge of burn management among nursing students at Basrah University, identifying gaps and correlations with sociodemographic factors. Methodology: A descriptive cross-sectional study was conducted among 150 nursing students at Basrah College of Nursing. Data was collected using a structured questionnaire divided into two sections: (1) sociodemographic information, and (2) burn management knowledge, assessed through a standardized 3-point Likert scale. Results: The majority of respondents (78% female, 22% male) were aged 19-28 years. The results revealed that students demonstrated a good level of knowledge regarding burn management. Furthermore, there was a highly significant correlation between the academic stage of the students and their test scores, suggesting that academic progression improves burn management understanding. Novelty: This study provides one of the first comprehensive assessments of burn care knowledge among nursing students in southern Iraq, highlighting the critical role of academic progression in enhancing practical burn care skills. Implications: The study suggests that incorporating practical burn care training into nursing education can significantly improve clinical outcomes in mass-burn scenarios. Highlights: Knowledge of burn care improves with academic progression among nursing students. Majority of participants were female, aged 19-28 years. Enhanced burn care training is essential in nursing education curricula. Keywords: Burn management, Nursing students, Knowledge assessment, Basrah University, Cross-sectional study

Music therapy with adult burn patients in the intensive care unit: short-term analysis of electrophysiological signals during music-assisted relaxation

Burn patients often face elevated pain, anxiety, and depression levels. Music therapy adds to integrative care in burn patients, but research including electrophysiological measures is limited. This study reports electrophysiological signals analysis during Music-Assisted Relaxation (MAR) with burn patients in the Intensive Care Unit (ICU). This study is a sub-analysis of an ongoing trial of music therapy with burn patients in the ICU. Electroencephalogram (EEG), electrocardiogram (ECG), and electromyogram (EMG) were recorded during MAR with nine burn patients. Additionally, background pain levels (VAS) and anxiety and depression levels (HADS) were assessed. EEG oscillation power showed statistically significant changes in the delta (p < 0.05), theta (p = 0.01), beta (p < 0.05), and alpha (p = 0.05) bands during music therapy. Heart rate variability tachograms high-frequencies increased (p = 0.014), and low-frequencies decreased (p = 0.046). Facial EMG mean frequency decreased (p = 0.01). VAS and HADS scores decreased − 0.76 (p = 0.4) and − 3.375 points (p = 0.37) respectively. Our results indicate parasympathetic system activity, attention shifts, reduced muscle tone, and a relaxed state of mind during MAR. This hints at potential mechanisms of music therapy but needs to be confirmed in larger studies. Electrophysiological changes during music therapy highlight its clinical relevance as a complementary treatment for ICU burn patients.Trial registration: Clinicaltrials.gov (NCT04571255). Registered September 24th, 2020. https//classic.clinicaltrials.gov/ct2/show/NCT04571255.

Development of N-alkylated benzimidazole based cubosome hydrogel for topical treatment of burns.

The current study focuses on assessing the activity of the N-alkylated benzimidazole based cubosomal hydrogel (cubogel) for the topical treatment of burn wounds. The study involves the synthesis of six benzimidazole derivatives (1-6) and their characterization by FT-IR and 1H and 13C NMR spectroscopy. The further study involves the design and formation of nanoparticles known as cubosomes loaded with selected 1-benzyl-1-benzimidazole (API 6) and the development of a cubogel for the topical treatment of burn wounds. Cubosomes were prepared by the homogenization method, using glyceryl monooleate (GMO) as a lipid polymer and poloxamer 407 (P407) as a surfactant. Cubosomes undergo in vitro characterizations (measurement of particle size, zeta potential, polydispersity index (PDI), % entrapment efficiency, drug release in phosphate buffer saline of pH 6.8, and surface morphology by utilizing TEM (transmission electron microscopy). Formulation D3 (2.5% of GMO, 1% of P407, and 2.5% of PVA) emerged as the optimized formulation, displaying a minimum particle size (PS) of 129.9 ± 1 nm, entrapment efficiency (%EE) of 96.67 ± 0.89%, and a drug release of 86 ± 2.7% at 24 h. Carbopol 940 hydrogel was prepared and incorporated with the optimized formulation to prepare cubogel. This optimized cubogel provided 92.56 ± 0.014% in vitro drug release within 24 h. An in vivo histopathological study was conducted on an animal model (rabbit) to assess the efficacy of cubogel in wound healing and wound contraction. Then cubogel was compared with the commercially available creams Clotrimazole® and Polyfax®. The wound treated with newly developed cubogel has maximum wound contraction (96.70%) as compared to the standard creams. The findings revealed that the newly formulated cubogel was highly effective in treating burns, showing superior performance to commercial products without inducing side effects. Additionally, benzimidazole derivative loaded cubogel caused a sustained release for treating burn wounds without any bacterial infections. The current results further suggested phase 0 clinical trials.

Arisarum vulgare: Bridging Tradition and Science Through Phytochemical Characterization and Exploring Therapeutic Potential via In vitro, In vivo, and In silico Biological Activities

AbstractArisarum vulgare O. Targ. Tozz. (Araceae), locally known as “Elbgouga,” holds significant traditional importance in Algeria for the treatment of various human ailments, including pain, infections, inflammation, digestive disorders, cancer, skin problems, eczema, wounds, and burns. The aim of this study was to explore for the first time the phytochemical profile, antioxidant, antibacterial, and inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, DNA protection, and capacity to promote wound healing. Preliminary phytochemical experiments were conducted to evaluate the major classes of bioactive compounds, in addition to the total phenol and flavonoid amounts in AVEE. An LC‐MS/MS analysis was conducted to clarify the phytochemical composition of this particular botanical species. The antioxidant capacity was assessed using DPPH· and ABTS·+ radical scavenging tests. The agar diffusion approach was used to ascertain the antibacterial efficacy against four bacteria (Staphylococcus aureus, Enterococcus faecium, Escherichia coli, and Salmonella typhimurium). Cholinesterase inhibition was evaluated using a colorimetric method that relies on Ellman's reaction. The protective effects of AVEE on pBR322 plasmid DNA damaged by H2O2 and UV treatment were assessed by their DNA‐breaking forms. The in vivo acute dermal toxicity and wound healing potential of the AVEE ointment (1–5% AVEO) were also investigated, and histological analyses were carried out on biological samples. Five protein targets were the subject of an in silico molecular docking investigation (TNFα, TGFBR1, IL‐1ß, GSK‐3ß, and NOS) and ADMET studies of the main components of the extracts were performed. The screening of phytochemicals revealed a significant concentration of phenolic compounds (176.00 ± 1.08 mg of gallic acid equivalents/g of dry extract), mainly flavonoids (81.21 ± 1.24 mg of quercetin equivalents/g of dry extract). Three major compounds were identified by LC‐MS/MS analysis, belonging mainly to the class of flavonoids, with rutin as the most abundant compound (11.85 mg/g extract), followed by hesperidin (4.125 mg/g extract). A conspicuous presence of isoquercitrin (1.327 mg/g extract) was also found. Small amounts of flavonoids and phenolic acids were also detected (catechin, chlorogenic acid, syringic acid, salicylic acid, kaempferol, and luteolin). DPPH assay showed higher antioxidant capacity compared to the ABTS·+ assay (142.60 ± 5.52 µg/mL and 236.10 ± 0.22 µg/mL, respectively). AVEE was effective against all selected bacterial strains; however, the highest zone of inhibition (36.00 ± 0.1 mm) was noted against E. faecium. The extract of the plant significantly inhibited both AChE and BChE (89.98 ± 18.76 µg/mL inhibitory activity against AChE and 98.28 ± 44.68 µg/mL inhibitory activity against BChE, respectively). The ethanolic extract of A. vulgare and quercetin exhibited more significant DNA protection action in form I, with percentages of 90.41 and 94.23%, respectively, compared to form II, where the percentages were 27.91 and 51.92%, respectively. The AVEO formulation may be safely administered by topical application. A statistically significant wound contraction was discovered in the group treated with 5% AVEO compared to the groups that were not treated or treated with petroleum jelly. Furthermore, there was no notable disparity detected between the group treated with 5% AVEO and the group treated with the reference drug. The 5% AVEO‐treated group showed, after the research was complete, the most significant percentage of wound contraction (96.90 ± 0.42%). Moreover, the topical application of the formulation improved histological parameters. In silico study showed that rutin, hesperidin, and isoquercitrin had a high affinity to the five main targets that might contribute to the wound healing potential of A. vulgare ethanolic extract. The impressive biological capabilities of A. vulgare indicate that the plant has the ability to be a valuable source of bioactive chemicals with various medical applications.

Comparative Efficacy of Silver Sulfadiazine and Aquacel Ag in Superficial Partial Thickness Burn Treatment: A Retrospective Analysis

Comparative Efficacy of Silver Sulfadiazine and Aquacel Ag in Superficial Partial Thickness Burn Treatment: A Retrospective Analysis

Topical application of anti-inflammatory agents on burn wounds and their effect on healing

Advancements in the treatment of burns have considerably improved overall survival rates, but they have also highlighted several long-term sequelae related to the injury. Hypertrophic scars can impair function, reduce quality of life, and require multiple procedures as well as physical therapy. The purpose of this study was to investigate the effects of topical application of anti-inflammatory drugs in the treatment of burns. Up to 15 deep-partial thickness burns were created on the dorsum of four anesthetized swine. Subsequently, the burn wounds were randomized to receive amiloride, celecoxib, dexamethasone or minocycline mixed in a hydrogel. Silver sulfadiazine cream and blank hydrogel acted as controls. The animals were followed for 90 days and the wounds were assessed on days 3, 7, 14, 28 and 90 post-burn. Assessments were performed using photographs (macroscopic healing, contraction), laser-speckle imaging (blood perfusion), 3D camera (scarring, pigmentation), and histology (inflammation, burn depth, epidermal maturation). Inflammation was present in all burn wound histology specimens and peaked on day 7 in all groups. Regardless of the treatment the burns progressed and were deeper on day 7 in comparison to day 3. The burns were 50 – 80 % healed by day 14, but no significant differences were observed. No differences in epidermal thickness, rete ridges, contraction, hypopigmentation, or scar elevation were seen on day 90. Topical anti-inflammatories did not significantly decrease inflammation or mitigate burn wound progression in deep partial thickness burns in pigs. Also, no significant differences in wound healing or quality of healing were observed.

A case-control study evaluating single and dual layer dermal matrix in treating scar contractures using the Vancouver and POSAS scar scales, and correlations with retraction and function in treating scar contractures

IntroductionThe dermal matrix appeared in the late 1990s and became an important tool in burn treatment. Following its success, new matrices soon emerged and were employed to treat burn contracture, but few studies have compared the different types available. This study evaluated two different dermal regeneration matrices comprised of one (Matriderm) or two layers (Integra) according to the Vancouver and the Patient and Observer Scar Assessment (POSAS) evaluation scales with regard to treatment of burn scar contracture. MethodsIn this prospective, case-control study, 24 patients with impaired mobility secondary to burns were divided into two groups and treated with dual or single layer matrices. Data were collected for intraoperative and postoperative complications, percentage take for matrix and graft areas, Vancouver scar scale score, POSAS score for skin quality assessment, area of matrix take, and skin graft take, and degree of mobility. Results and conclusionBoth matrices produced significant improvement according to the Vancouver and POSAS assessments over 12 months, but the dual layer matrix yielded better results than the single layer matrix in terms of retraction of the grafted area at 12 months, functional improvement, and skin quality as measured by the Vancouver and POSAS scales. According to statistical analysis, with p<0.05.

Two new flavonoids from the leaves of Garcinia smeathmannii, in vitro and in silico anti-inflammatory potentials

Garcinia smeathmannii is a well-known plant for its uses in the effective treatment of intestinal parasites, skin eruptions and skin burns. The dichloromethane-methanol (2:3) crude extract of the leaves of G. smeathmannii led to the isolation and characterization of twenty compounds (1−20) using chromatographic and spectroscopic techniques. Extracts and compounds were screened in vitro for their anti-inflammatory (ROS), antiglycation and antileishmanial (L. tropica) activities. Compounds were also screened for their in silico anti-inflammatory activities using Maestro 4.2.1 software with the co-crystal complex structures of the ovine oCOX-1: meloxicam (PDB Id: 4O1Z) and murine mCOX-2: meloxicam (PDB Id: 4M11) proteins. An unprecedented flavonol (1) and a flavone dimer (2) together with eighteen known compounds (3−20) were characterized. All the tested samples in vitro revealed no antiglycation and antileishmanial activities. Beside, extracts revealed moderate anti-inflammatory activities (IC50 ranging from 24.1 ± 2.0 to 34.7 ± 0.8 μg/mL). Only compound (13) revealed an anti-inflammatory activity which was 9.33 times more active than the reference (Ibuprofen, IC50 = 11.2 ± 1.9 μg/mL) with IC50 of 1.2 ± 0.0 μg/mL. Compounds (2–9, 11–13 and 19–20) were docked and the docking scores were ranging from −10.178 to −6.119 (kcal/mol) which were in agreement with the experimental anti-inflammatory activity. These results are in agreement with the traditional uses of the leave of G. smeathmannii as cataplasm for skin eruption and as analgesic agent.

Cost Analysis of Pure Hypochlorous Acid Preserved Wound Cleanser versus Mafenide for the Irrigation of Burn Wounds.

Over 40,000 patients in the United States (US) require hospitalization for burns annually. The treatment regimen can cost more than $6,000 a day and requires the use of numerous supplies to ensure the graft takes for successful wound healing. Irrigation of the wound is a critical step for burn treatment, yet little is known about the cost-effectiveness of different irrigation modalities. In a recent study, pure hypochlorous acid preserved wound cleanser (pHA) was shown to be safe and effective compared to mafenide. This study estimated the associated costs of two common wound irrigation modalities, pHA and mafenide solution, for the treatment of patients with burns. In this study, a patient-level Monte Carlo simulation model using data from a randomized control trial (RCT) was used to conduct the cost analysis from the US Hospital perspective. Based upon 100,000 simulated patients, pHA was expected to save $133 ($123 to $144, 10th to 90th percentile) for the hospital compared to using a mafenide solution over 14 days. Adoption of pHA should be considered a cost-saving strategy when treating patients with burns.

Achieving The Nutritional Goal Of Burn Patients In A Referral Hospital In The Northern Region Of Brazil

Background: Burns represent a significant health challenge, not only due to the pain and suffering they cause, but also due to metabolic complications. Oral nutritional therapy is a powerful tool in the recovery of these patients, as the burn requires double the energy demands. Therefore, achieving the nutritional goal in burn patients should be considered a priority in clinical management. Materials and Methods: Patients admitted to the Burn Treatment Center at a reference hospital in the North region were evaluated. For nutritional diagnosis, the gold standard tools for nutritional risk analysis were applied, Nutritional Risk Screening (NRS-2002) for adults and Screening Tool for Risk on Nutritional Status and Growth (Strongkids, 2012) for children. These are validated low-cost and non-invasive methods. Monitoring the achievement of VET and protein was carried out through food records and the percentages achieved in accordance with the prescribed calories and proteins were calculated. Results: 22 adult patients were evaluated. Of these, 15 men and 7 women, with an average age of 35.8 years. The assessment revealed 7% malnourished, 67% eutrophic, 33% obese and 13% obese. Adults averaged 2026 cal/day with 112% VET attainment and 90g protein with 125% intake. It is worth highlighting the joint action of nutrition professionals with the prescription of hypercaloric and hyperprotein preparations to adapt to the needs recommended for burn patients, in addition to Oral Nutritional Therapy (ONT), with an increase of 600cal/day and 40g of proteins. The use of hypercaloric and hyperprotein supplementation in burn patients is a strong ally in diet therapy, given the catabolic state and high risk of depletion. Among the children's audience, 7 children were served: 4 boys and 3 girls, an average age of 4.4 years. The nutritional diagnosis showed 85% in eutrophy, and 15% in nutritional risk. The VET of this group was on average 1215 cal and 39g of protein. Children achieved an average of 142% of daily VET and 175% of protein needs. To this end, 85% used TNO, accounting for 300 cal and 7.6 g of additional protein to the oral diet. Conclusion: Achieving the nutritional goals prescribed for burn patients has a significant impact on the meffectiveness of treatment

Measurement of thermophysical parameters of artificial skin

Computer methods for process research and methods for processing the measurement results of the parameters of these processes are widely used in biology and medicine. Therefore, the problem of automation of biophysical measurements and computer processing of the results of such experiments is relevant. In the treatment of wounds and burns with a large surface area of tissue damage, medicine has to resort to artificial skin. A procedure for studying thermal processes in artificial skin and measuring its thermophysical parameters was developed. The measurement process was described. The thermophysical parameters of the artificial skin samples used in medicine for the treatment of wounds and burns were determined. The samples of the artificial skin used were made in Ukraine. Each sample was made from six layers of skin. To control the temperature distribution in the sample between the skin adjacent layers and on the surface of the sample, thermocouples were placed. The surface of the sample was heated with an incandescent lamp. The thermocouple signals were sent to an analog-to-digital converter (ADC), amplified, and digitally transmitted to a computer. Processing was carried out using the ADC software and the MATHCAD program. A mathematical model was obtained. For this purpose, a differential equation describing thermal processes in the sample was solved. The found temperature distribution function and its changes over time are in a good agreement with experimental data. The analysis of the function allows finding the thermal conductivity coefficient of artificial skin, its volume heat capacity, thermal diffusivity coefficient, and heat exchange coefficient with the external environment. The proposed method of measuring and processing experimental data makes it possible to detect main thermophysical parameters of artificial skin. Based on these results, a theoretical model was obtained that well describes the course of thermal processes in artificial skin, which makes it possible to predict the course of these processes. Such method is based on heating the skin with optical radiation. The thermal conductivity and thermal diffusivity of artificial skin are significantly less than such parameters of human skin, which should be considered when using it.

Enhanced Third Degree Burn Wound Healing by Hypoxic Mesenchymal Stem Cells' Secretome: IL-10 Upregulation and TNF-α/PGE2 Suppression

Background: Severe third-degree burns pose a notable obstacle in clinical settings, frequently leading to hindered healing of wounds and the development of scars. In this research, we explored how the secretome of hypoxic mesenchymal stem cells (HS-MSCs) could be improved the healing of burn wounds. Methods: In this study, we used 20 male Wistar rats that induced third degree wound by metal 80 exposure for 10 s. These rats divided into 4 groups: Healthy, control, HS-MSCs 100 µL, and HS-MSCs 200 µL groups. We injected the HS-MSCs, which carry various beneficial molecules like IL-10, TGF-β, PDGF, and VEGF, directly into the skin in a third-degree burn rat model on day 1 after burn induction, and harvest on day 14. Results: Our research discovered that using HS-MSCs resulted in a dosage-related rise in IL-10, an anti-inflammatory cytokine, alongside a notable decrease in TNF-α and PGE2, pro-inflammatory cytokines. These effects quickened the healing process by improving re-epithelialization and minimizing scar formation. Conclusions: Our findings indicate that HS-MSCs show promise as a treatment for severe burns by potentially controlling inflammation and fostering a regenerative healing process. More investigation is needed to understand the exact mechanisms and to explore how HS-MSCs could be applied in clinical treatments. HIGHLIGHTS HS-MSCs suppress inflammation and promote regenerative healing in severe burns HS-MSCs accelerated re-epithelialization and reduced scarring GRAPHICAL ABSTRACT

The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study.

Background/Objectives: The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. Methods: The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm2). Radical necrectomy was performed one day after the burn. Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel). The treatment lasted for five days. The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods. Results: The differences in the rate of wound healing and the characteristics of wound cytology were identified. In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples from non-treated and mock-treated animals. Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels. The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity. The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups). A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment. Conclusions: The cell product is an effective agent for promoting wound healing during the regenerative phase. The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds.

Exosomes and microRNAs: insights into their roles in thermal-induced skin injury, wound healing and scarring.

A burn is a type of injury to the skin or other tissues caused by heat, chemicals, electricity, sunlight, or radiation. Burn injuries have been proven to have the potential for long-term detrimental effects on the human body. The conventional therapeutic approaches are not able to effectively and easily heal these burn wounds completely. The main potential drawbacks of these treatments include hypertrophic scarring, contracture, infection, necrosis, allergic reactions, prolonged healing times, and unsatisfactory cosmetic results. The existence of these drawbacks and limitations in current treatment approaches necessitates the need to search for and develop better, more efficient therapies. The regenerative potential of microRNAs (miRNAs) and the exosomal miRNAs derived from various cell types, especially stem cells, offer advantages that outweigh traditional burn wound healing treatment procedures. The use of multiple types of stem cells is gaining interest due to their improved healing efficiency for various applications. Stem cells have several key distinguishing characteristics, including the ability to promote more effective and rapid healing of burn wounds, reduced inflammation levels at the wound site, and less scar tissue formation and fibrosis. In this review, we have discussed the stages of wound healing, the role of exosomes and miRNAs in improving thermal-induced wounds, and the impact of miRNAs in preventing the formation of hypertrophic scars. Research studies, pre-clinical and clinical, on the use of different cell-derived exosomal miRNAs and miRNAs for the treatment of thermal burns have been documented from the year 2000 up to the current time. Studies show that the use of different cell-derived exosomal miRNAs and miRNAs can improve the healing of burn wounds. The migration of exosomal miRNAs to the site of a wound leads to inhibition of apoptosis, induction of autophagy, re-epithelialization, granulation, regeneration of skin appendages, and angiogenesis. In conclusion, this study underscores the importance of integrating miRNA and exosome research into treatment strategies for burn injuries, paving the way for novel therapeutic approaches that could significantly improve patient outcomes and recovery times.