Burnetii Antibodies Research Articles

Seroepidemiology of Coxiella burnetii in Domestic and Wild Ruminant Species in Southern Spain

The European Food Safety Authority has recently listed Q fever as a priority for setting up a coordinated surveillance system. Although Spain is the country with the highest human incidence of the disease in the European Union, updated data on Coxiella burnetii in ruminants are still limited. A total of 780 serum samples from small ruminants and 605 sera from wild ruminants were collected in the Mediterranean ecosystems of southern Spain during the period 2015–2023. Anti-C. burnetii antibodies were detected using a commercial indirect ELISA. The overall individual seroprevalence in the small ruminants was 49.1% (383/780; 95% CI: 45.6–52.6). Antibodies against C. burnetii were identified in 40.0% (156/390) of sheep and in 58.2% (227/390) of goats. At least one seropositive animal was observed in all sheep (100%) and in 92.3% of goat flocks. The species (goat) and the existence of reproductive disorders in primiparous females were potential risk factors for C. burnetii exposure in small ruminant farms. In the wild ruminants, the overall seroprevalence against C. burnetii was 1.5% (9/605; 95% CI: 0.8–2.8). Anti-C. burnetii antibodies were found in 1.8% (2/110) of mouflon, 1.5% (6/390) of red deer, and 1.0% (1/105) of Iberian ibex. The high exposure of the small ruminants to C. burnetii, particularly in goats, detected in the present study is of animal and public health concern. Our results denote that wild ruminants only play a minor role in the epidemiology of this bacterium in southern Spain and suggest an independent epidemiological cycle of C. burnetii in domestic and wild ruminant species in the study area.

Q Fever in Greece and Factors of Exposure: A Multiregional Seroprevalence Study.

The epidemiology of Q fever, caused by Coxiella burnetii, varies significantly worldwide. This study aimed to document the prevalence of Coxiella burnetii in Greece by measuring specific IgG antibody levels in serum samples from the general population and high-risk groups, including farmers, veterinarians, and laboratory workers. A multiregional, stratified sampling design was employed, with 1,345 participants from Thessaly and Central Macedonia. Serum samples were tested for Coxiella burnetii IgG antibodies, and multivariate analysis was conducted to identify factors associated with seroprevalence. Overall, 8.1% of participants tested positive for Coxiella burnetii antibodies, with the highest seroprevalence in Larissa (22.2%) and Karditsa (16.1%). High-risk occupational groups, particularly those with direct animal contact, showed a higher seroprevalence (13.6%). Multivariate analysis identified significant associations between seroprevalence and factors such as geographic region, occupation, and gender. The study reveals regional and occupational disparities in Q fever seroprevalence in Greece, particularly in rural areas. These findings underscore the need for targeted public health measures, including heightened surveillance and preventive interventions for high-risk groups.

A sero-epidemiological analysis of Coxiella burnetii infection and its risk factors in livestock from Addis Ababa, Adama, and Modjo abattoirs and pastoral areas of Oromia, Ethiopia.

Coxiella burnetii is causing infections in both humans and animals, resulting in Q fever and Coxiellosis, respectively. Information on the occurrence of C. burnetii infection is scarce in Ethiopia. This study estimated the sero-prevalence of C. burnetii infection and associated risk factors in four common livestock species from Addis Ababa, Adama, and Modjo abattoirs and pastoral areas of Oromia, Ethiopia. Sera samples were analyzed for the presence of anti-C. burnetii antibodies using an indirect Enzyme Linked Immunosorbent Assay kit. Out of the 4140 serum samples tested, 777 (18.77%; 95% CI: 17.59, 19.99) were found positive for C. burnetii. The sero-prevalence estimate was 27.17% at Addis Ababa abattoir, 19.41% at Adama abattoir, 19.13% at Modjo abattoir and 12.1% in animals tested from pastoral areas. Sera analysis at the animal species level showed that cattle exhibited the lowest sero-prevalence estimate (11.83%; 95% CI, 10.27-13.53%), while the highest was observed in camels (28.39%; 95% CI, 25.16-31.80%). The sero-prevalence estimate was 21.34% (95% CI, 18.86-23.99%) in goats and 20.17% (95% CI, 17.49-23.07%) in sheep. The results of multivariable logistic regression analysis showed that species, age, sex of animals and tick infestation were important risk factors for C. burnetii infection. The odds of infection were 3.22 times higher in camels and almost twice as high in goats and sheep compared to cattle. Adult animals were infected more likely (OR = 3.23) than young ones. Interestingly, a significant difference was observed in the sero-prevalence of infection between animals that were infested with ticks (OR = 16.32) and those which were tick-free. This study provides valuable insights into the sero-epidemiology of C. burnetii infection in four common livestock species at major abattoirs and pastoral areas of Ethiopia. The findings highlight the need for further studies and implementing surveillance and biosecurity measures to prevent the spread of the disease in both humans and livestock to safeguard the economical and public health aspects.

Seroprevalence of Coxiella burnetii and potential tick vectors infesting domestic ruminants and community perception of the disease in pastoral areas of south Omo zone, southern Ethiopia

BackgroundQ fever is a worldwide occurring neglected zoonotic disease with great economic importance. The etiological agent, Coxiella burnetii, is a bacterium usually associated with subclinical infections in livestock, but may also cause reproductive pathology and spontaneous abortions in artiodactyl species including goats, sheep and cattle which are deemed to be the primary reservoirs of this disease. AimsThe present cross-sectional and questionnaire survey was undertaken in three districts of the South Omo zone with the aims to comprehend the community perception of livestock keepers and professionals about the disease, estimate the seroprevalence of Coxiella burnetii (C. burnetii) in cattle and small ruminants and to determine the species of potential tick vectors of C. burnetii infesting cattle, sheep and goats. MethodsA standard questionnaire was used to assess the community perception of livestock keepers and animal health professionals in the area about Q fever. Sera samples were collected from 1350 ruminants comprising 450 cattle, 450 goats and 450 sheep to detect C. burnetii antibodies using the ELISA technique. Furthermore, a total of 279 cattle, 197 goats and 73 sheep were examined for the presence of ticks, and overall, 2720 ticks were collected (1299 from cattle, 1020 from goats and 401 from sheep) and identified to the species level using morphologically identification keys. ResultsFindings of the study indicated that 43% of animal owners were aware of the main symptoms of the disease while the remaining 57% did not notice these symptoms in their animals. Additionally, majority of animal health professionals 76.2% in the area reported they were familiar with the causative agent of Q fever, while 23.8% expressed uncertainty regarding the cause of coxiellosis. An overall seroprevalence of C. burnetii of 37.6% in cattle (37.4% in female and 37.8% in male cattle) and 28.7% in small ruminants was recorded (which is significantly higher in goats than in sheep). The study indicated statistically significantly higher seroprevalence of C. burnetii (49.8%) in cattle infested with ticks than in those cattle free of ticks (24.2%), with three times higher seropositivity (OR = 2.97, p = 0.000) as compared to those cattle free of ticks (24.2%). Similarly, statistically significantly higher seroprevalence of C. burnetii was recorded in both sheep and goats infested with ticks (43.6%) as compared to those animals without ticks (22.9%), with the former being twice as likely to test seropositive (OR = 2.15, p = 0.000). A total of nine different tick species were identified, namely Amblyomma variegatum (Am. variegatum) with 26.3% (342; 217 males, 101 females and 24 nymphs), Amblyomma cohaerens (Am. cohaerens) with 47.96% (370 males, 253 females), Amblyomma gemma (Am. gemma) with 4.00% (52; 29 males, 23 female), Rhipicephalus pulchellus (Rh. pulchellus) with 10.6% (138; 87 males, 51 females), Rhipicephalus pravus (Rh. pravus) with 0.2% (3; 2 males, 1 females), Rhipicephalus evertsi (Rh. evertsi) with 4.7% (61; 39 males, 22 females), Rhipicephalus praetextatus (Rh. praetextatus) with 0.8% (10; 7 males, 3 females), Rhipicephalus decoloratus (Rh decoloratus) with 2.9% (38; 4 males, 34 females) and Hyalomma truncatum (Hy. truncatum) with 2.5% (32 females). ConclusionThe present study highlighted the significance of Q fever in ruminants and compiled information about the community perception of livestock keepers and veterinary professionals of the study areas. The role of ruminants and their ticks in the epidemiology of C. burnetii requires further research using molecular tools to better understand appropriate method of intervention that will help to reduce negative impacts on the productivities of livestock and the health of humans in Ethiopia.

Seroprevalence of Q fever (Coxiella burnetii) in sheep in the Kwahu West municipality, Eastern Region, Ghana

Query fever, also known as Q fever, is a zoonotic disease caused by Coxiella burnetii. It is a cause of abortion in livestock and presents as a febrile illness in humans. A correlation between the incidence of the disease in humans and abortion in goats and sheep farms has been reported in countries such as the Netherlands and Australia. In Ghana, the occurrence of Q fever in both livestock and humans has not been fully explored. This study sought to determine the seroprevalence of Q fever in livestock in Nkawkaw, in the Eastern Region of Ghana. Sera obtained from 92 sheep from 12 farms were tested using the indirect multi-species ELISA for the detection of anti-Coxiella burnetii antibodies. Animal demographics, farms’ proximity to human settlement and history of abortion in relation to the Q fever status were assessed.The overall prevalence of Q fever was 13.0 % [95 % CI 6.9–21.6] (12/92). Both sexes were equally affected, with a sex-specific prevalence of 13.0 % each. The farm-specific prevalence was 50 %. Abortions were reported on eight (8) of the 12 farms, and all farms were located less than 200 m from human habitation. Only proximity of farm to human settlement showed statistical significance. Q fever is prevalent in Nkawkaw and requires the attention of both animal and health authorities, using the One- Health approach to nip any future epidemics in its bud.

Acute Q fever revealed by an anti-phospholipid syndrome: A case report

IntroductionQ fever is a zoonosis caused by Coxiella burnetii. Acute infection is mainly asymptomatic. In other cases it mainly causes a flu-like illness, a pneumonia, or an hepatitis. We present an atypical case of an acute Q fever revealed by a massive pleural effusion. Case reportWe report the case of a 43-year-old man referred to our hospital for an acute respiratory distress. Further analyses showed an exudative eosinophilic pleural effusion, associated with a pulmonary embolism and a deep femoral vein thrombosis. Aetiologic explorations revealed an acute Q fever (IgM and IgG against C. burnetii phase II antigens) associated with anti-phospholipids. The outcome was favorable with vitamin K antagonists, doxycycline, and hydroxychloroquine, till the negativation of the anti-phospholipid antibodies. Discussion and conclusionDuring acute C. burnetii infections, anti-phospholipid antibodies are highly prevalent but thrombotic complications are rare. The 2023 ACR/EULAR APS criteria restricts the diagnosis of APS, as in our case of acute severe infection. In front of an atypical pneumonia and/or thrombotic events, screening of C. burnetii and anti-phospholipid antibodies could be useful. Given its low level of evidence, prolongated treatment by doxycycline, hydroxychloroquine ± anticoagulant for C. burnetii's associated anti-phospholipid syndrome is discussed, but succeeded in our case.

One Health approach to Coxiella burnetii in Brazilian indigenous communities

Indigenous health has posted complex challenges worldwide, particularly due to historical economic, territorial, social and environmental processes, which may lead to emergence and reemergence of pathogens. In addition to few Coxiella burnetii serosurveys in vulnerable populations, especially in developing tropical countries, no comprehensive One Health approach has focused on human-animal infection along with potential environmental determinants. Accordingly, this study aimed to assess the seroprevalence of anti-C. burnetii antibodies in indigenous populations and their dogs from 10 indigenous communities distributed in southern and southeastern Brazil, along with the correspondent healthcare professionals. In overall, 8/893 (0.90%; 95% CI 0.45–1.76) indigenous and 1/406 (0.25%) dog samples were seropositive, with 7/343 (2.04%) individuals the 1/144 (0.69%) dog from the Ocoy community, located in the city of São Miguel do Iguaçu, bordering Argentina at south, and far 10 km at west from Paraguay. All 84 healthcare professionals tested seronegative.

Serosurvey of Coxiella burnetii in Police Officers and Working Dogs in Brazil: Case Report and One Health Implications.

Although the Coxiella burnetii infection has been investigated in dogs, its role in human transmission remains to be fully established, particularly in close and daily human-dog contact settings, such as in Police K-9 Units. Accordingly, this study aimed to assess anti-C. burnetii antibodies in clinically healthy police officers by an in-house indirect immunofluorescence assay (IFA), and working dogs by a commercial IFA Kit, from the State Special Operations Battalion, Paraná, Southern Brazil. Overall, 1/18 (5.5%) police officers and 9/30 (30.0%; CI 95% 16.66-47.88) dogs tested seropositive to anti-C. burnetii IgG antibodies. To date, this is the highest prevalence of Q fever seropositivity among military dogs worldwide. Despite the low sampling rate, a statistically significant association was found between seropositivity and female dogs (p = 0.0492). Further studies with larger sample sizes should be conducted to establish the prevalence of Q Fever in other Brazilian K-9 Units. In summary, this study is the first to conduct a concomitant serosurvey of police officers and working dogs, and its findings should be considered a warning for cross-exposure and transmission of Coxiella burnetii among Police K-9 Units in Brazil and worldwide.

Exposure of small ruminants and humans to Coxiella burnetii in the semi-arid region of Northeastern Brazil.

Q fever is a zoonotic disease caused by the obligate intracellular pathogen Coxiella burnetii, for which domestic ruminants are the primary source of infection in humans. Herein, we investigated the presence of C. burnetii in humans, sheep, and goats in the semi-arid region of northeastern Brazil. The presence of anti-C. burnetii antibodies was surveyed using indirect immunofluorescence assay, and detection of C. burnetii DNA was performed by polymerase chain reaction (PCR). Anti-C. burnetii antibodies were detected in 60% of farms, 4.8% of goats, 1.5% of sheep, and 4.5% of human samples. PCR was positive in 18.9% of blood samples, 7.7% of milk samples, and 7.7% of vaginal mucus samples. A DNA sequence of a C. burnetii DNA sample extracted from the goat vaginal mucus showed 99.2-99.4% nucleotide identity with other strains previously reported in Brazil. These results indicate that C. burnetii is present in the surveyed area, where it poses a risk to both public and animal health. These findings indicate an urgent need for educative actions to protect population, as well as better training of veterinarians to detect and report Q fever.

Prevalence and associated risk factors of anti-Coxiella burnetii antibodies in dairy cattle herds using bulk tank milk analysis in Kabylia area, north Algeria.

Coxiella burnetii, or Q fever agent, has notable implications for human and livestock health. Infections in cattle primarily manifest through reproductive issues where infected animals shed the bacterium in birth fluids, placental tissues, and milk, serving as potential sources of transmission. Bovine herds become reservoirs, contributing to the environmental contamination of farming areas. Comprehensive studies on the prevalence, transmission routes, and associated risk factors among cattle contribute to the development of effective control strategies, ultimately safeguarding both livestock and public health.Here we determine the prevalence of Coxiella burnetii antibodies against in dairy cattle farms from Kabylia (northern Algeria) and identify the associated risk factors. Bulk tank milk samples from 184 farms were analyzed by indirect ELISA technique, 49 of them were tested positive which corresponds to a prevalence rate of 26.63% (95% CI 20.25-33.01%). Multivariate analysis by logistic regression showed that the risk factors associated with detection of anti-Coxiella burnetii antibodies are: cohabitation of cattle with small ruminants(OR = 3.74 95% CI [1.41-8.92]), exposure to prevailing winds (OR = 5.12 95% CI [2.11-13.45]), and the veterinarian visits frequency(OR = 5.67 95% CI [2.55-13.60]). These findings underscore the susceptibility of dairy cattle to Q fever in the Kabylia region, highlighting practices that pose risks. We recommend the implementation of hygienic measures and adherence to proper farming conditions to mitigate the transmission of Q fever and reduce the associated zoonotic risk.

Serosurvey of Coxiella burnetii in Descendants of Former Black Slaves (Quilombola Communities) of Southern Brazil.

Brazilian descendants of former Black-slave (quilombola) communities have been predisposed to several zoonotic diseases due to social vulnerability, characterized by subsistence and close contact with livestock and companion animals. Accordingly, the present study has assessed anti-Coxiella burnetii antibodies in 200 individuals and 20 dogs from four quilombola communities located in Paraná State, southern Brazil. Serum samples were tested by indirect immunofluorescence assay (IFA) using in-house and commercial diagnostic protocols, with analysis of seropositive titers and antibody type. Fisher's exact test was used to compare seropositivity to C. burnetti with binary variables, with variables with three or more possible responses submitted to logistic regression. In total, 44/200 (22%; 95% CI 16.82-28.24) people tested positive, and 4.5% had titers higher than 128, indicating a recent onset of C. burnetii infection. Seropositive individuals were statistically associated with the Limitão community (p = 0.0013), urban workers as occupations (p = 0.0475), consumption of undercooked meat (p = 0.0159), and contact with animal abortion (p = 0.0276). No seropositivity association was found for age, sex, education, habit of entering forest areas, consumption of game meat, consumption of raw milk, flea and tick bites, dog contact, or history of female miscarriage. Only one of 20 dogs was seropositive with a titer of 128, probably related to an acute animal infection. Despite the prevalence here being higher than previous Brazilian reports, including with symptomatic populations, the results were within range for worldwide outbreaks and occupational risk populations. To the reader's knowledge, this is the first human survey of Q fever in southern Brazil and should be considered a warning for C. burnetii in vulnerable populations, particularly Quilombola communities.

Coxiella burnetiid seroprevalence, risk factors, and health hazards in sheep and goats in Upper Egypt

Query fever (Q fever) or coxiellosis is a serious bacterial infection caused by Coxiella (C.) burnetii and affects various animals and humans. Clinically, Q fever ranges from various degrees of fever to abortion, either in infected animals or humans. Such infection is especially important in cattle and small ruminants industry, particularly sheep and goats. Thus, the current study aimed to recognize the prevalence of specific antibodies against C. burnetii in serum samples collected from sheep and goats in Sohag governorate, Upper Egypt, using a commercially available enzyme-linked immunosorbent assay (ELISA). The overall seroprevalence was found against C. burnetii (25.6%; 56/219), subdivided as 22.8% (23/101) in sheep and 28% (33/118) in goats. Animals used for this study are representative of different small ruminants (sheep and goats), age (various ages), sexes (males and females), locations (different localities in Sohag governorate), physiological and pathological conditions, and many other factors related to animals, farm, and the environment. Female animals exhibited a higher rate of C. burnetii antibodies than males (p = 0.0637). Also, females in the dry period showed a higher seropositive rate than those pregnant females (p < 0.0001). In addition, the breeding system was reported as a risk factor for infection because animals bred in smallholders demonstrated a lower prevalence rate than those reared in individual breeding (p= 0.010) and mass farming (p= 0.006). Clinical and biochemical variables were estimated to recognize the health impact of seropositivity. Seropositive animals exhibited marked alterations in the selected clinical parameters and alanine transaminase (ALT) compared to the seronegative ones. Determining the exact occurrence of Q fever in sheep and goats might assist in developing a control policy for this infection and thus improve the income of the small ruminants industry and protect humans from infection.

Coxiella burnetii seroprevalence in sheep herd from Paraguay: First evidence of bacterial circulation in the country

Coxiella burnetii is the agent of Q fever, a disease that poses risks to public health and damages livestock. We discovered the circulation of C. burnetii for the first time in Paraguay, based on the seropositivity of a flock of >300 sheep. The animals were tested by IFA for anti-C. burnetii antibodies and by SAM for anti-Leptospira spp. antibodies, an important differential diagnosis for reproductive disorders in sheep in Paraguay. C. burnetii seropositivity was determined in 45%, in contrast to Leptospira spp. which had no reactive samples. Cases of miscarriage and fetal resorption were associated with high seropositivity titers. This study suggests the circulation of a unique genotype in the country and an imminent risk to public health, since in addition to being highly transmissible and infectious to humans, Q fever is still not a cause for concern on the part of government and health agencies in the country.

Distribution and Prevalence of Coxiella burnetii in Animals, Humans, and Ticks in Nigeria: A Systematic Review.

'Query' (Q) fever is a neglected but emerging or re-emerging zoonotic disease caused by the bacterium Coxiella (C.) burnetii. Several host species are considered or speculated to be the primary reservoir hosts for human infection. In the past, several research groups in Nigeria have evaluated the prevalence of C. burnetii in various vertebrate and invertebrate hosts. Currently, there is a paucity of knowledge regarding the epidemiology of the pathogen in Nigeria with limited or no attention to control and prevention programs. Therefore, this review was undertaken to comprehend the current situation of C. burnetii infection in human, domestic and peri-domestic animals, and some tick species in Nigeria since 1960 with the aim to help identify future research priorities for the country. A comprehensive literature search was performed using the PRISMA guidelines on five scientific databases including Google Scholar, PubMed, AJOL, Science Direct, and Scopus for articles published from Nigeria dealing with the screening of blood, milk, or tick DNA for evidence of C. burnetii using any standard diagnostic approach. Of the 33 published articles subjected to full-text evaluation, more than 48% of the articles met the inclusion criteria and were thus included in this review. We observed different ranges of prevalence for C. burnetii antibodies from four vertebrate hosts including cattle (2.5-23.5%), sheep (3.8-12.0%), goats (3.1-10.9%), and humans (12.0-61.3%). Additionally, the use of molecular diagnostics revealed that the DNA of C. burnetii has been amplified in eight tick species including Hyalomma (Hy) dromedarii, Hy. truncatum, Hy. impeltatum, Hy. rufipes, Hy. impressum, Amblyomma (Am.) variegatum, Rhipicephalus (Rh.) evertsi evertsi, and Rh. annulatus. Two rodent's species (Rattus rattus and Rattus norvegicus) in Nigeria were documented to show evidence of the bacterium with the detection of the DNA of C. burnetii in these two mammals. In conclusion, this review has provided more insight on the prevalence of C. burnetii and its associated host/vector in Nigeria. Domestic animals, peri-domestic animals, and ticks species harbor C. burnetii and could be a source of human infections. Due to the paucity of studies from southern Nigeria, we recommend that research groups with interest on vector-borne diseases need to consider more epidemiological studies in the future on C. burnetii prevalence in diverse hosts to help unravel their distribution and vector potentials in Nigeria as a whole.

Q fever and toxoplasmosis in South African livestock and wildlife: a retrospective study on seropositivity, sporadic abortion, and stillbirth cases in livestock caused by Coxiella burnetii

BackgroundQ fever and toxoplasmosis are economically important zoonoses as they cause considerable losses in livestock (cattle, sheep and goats) and wildlife (antelopes, giraffes, lions, and cheetahs) through reproductive disorders such as abortions and stillbirths. Q fever and toxoplasmosis testing in South Africa is conducted by the Agricultural Research Council-Onderstepoort Veterinary Research (ARC-OVR). However, both zoonoses are understudied and not monitored in South Africa as they are not considered controlled or notifiable diseases in the Animal Disease Act 35 of 1984. A retrospective study was conducted on Q fever (2007–2009) and toxoplasmosis (2007–2017) using diagnostic laboratory data at the ARC-OVR. Also, we report on sporadic abortion and stillbirth cases in livestock from diagnostic tissue samples submitted for Coxiella burnetii polymerase chain reaction (PCR) detection at the ARC-OVR.ResultsDuring 2007 to 2009, 766 animal samples were tested for C. burnetii antibodies and seropositivity was 0.9% (95%CI: 0.3–1.7) with sheep (1.9%; 95%CI: 0.6–4.4) having the highest seropositivity followed by cattle (0.7%; 95%CI: 0.09–2.6), while all goats (0.0%; 95%CI: 0.0–4.2) and wildlife (0.0%; 95%CI: 0.0–2.5) tested were negative. From 2007 to 2017, 567 sera were tested for T. gondii antibodies; overall seropositivity was 12.2% (95%CI: 9.6–15). Wildlife had highest seropositivity to T. gondii antibodies (13.9%; 95%CI: 9.0–19.7) followed by goats (12.9%; 95%CI: 9.2–17.4) and sheep (12.3%; 95%CI: 5.1–23.8) while seropositivity in cattle was 2.4% (95%CI: 0.06–12.9). Of 11 animals tested by C. burnetii PCR detection (2021–2022), 10 (91.0%) were positive. The amplicon sequences showed similarity to Coxiella burnetii strain 54T1 transposase gene partial coding sequence.ConclusionsWe have confirmed the occurrence of the causative agents of Q fever and toxoplasmosis in livestock and wildlife in South Africa, with data limitations. These zoonoses remain of importance with limited information about them in South Africa. This study provides baseline information for future studies on Q fever and toxoplasmosis in South African livestock and wildlife, as well other African countries. Due to limited data collection experienced in this study, it is recommended that improvements in data collection samples tested should include associated factors such as sex, age, and breed of the animals.

Antibody-Based Assessment of Coxiella burnetii Circulation in Algerian Goat Herds.

Q fever is a zoonotic disease caused by Coxiella burnetii (C. burnetii), a pathogen with a high capability for infection. The disease primarily affects ruminants, leading to reproductive disorders, but can also be transmitted to humans through contact with infected animals or their products. In Algeria, Q fever is endemic, but little is known about the presence and circulation of C. burnetii in domestic goats. This study aimed to perform a multicentric serological analysis of C. burnetii antibodies in domestic goats from four provinces in the North East Region of Algeria. A total of 504 goat serum samples were collected from 77 herds, and serological analysis was performed using an indirect ELISA. The overall seroprevalence at the herd level was 35.06%, and 8.73% at the individual level. Herds with a history of abortions showed a high seropositivity rate of 88.9%. This research indicates the wide distribution of C. burnetii in goats in this region, suggesting the potential for zoonotic transmission to humans. Further studies and monitoring programs are essential to gain a comprehensive understanding of C. burnetii epidemiology in Algeria and to prevent or mitigate potential outbreaks. Awareness among practitioners and farmers is crucial to address this public health concern effectively.

Epidemiological investigation of Coxiella burnetii in cattle and its association with Ixodid tick infestation in different agro-ecological zones of Southwest Ethiopia

Coxiella burnetii is a serious zoonotic disease that causes significant economic losses in cattle production, including abortion, stillbirth, infertility, and reduced milk yield. However, little is known about the epidemiology of C. burnetii in Ethiopia. From November 2020 to November 2021, a cross-sectional study was conducted to estimate the seroprevalence and associated risk factors of C. burnetii in cattle in various agro-ecologies of Southwest Ethiopia. Blood samples were collected from 461 cattle, and the serum samples were tested for the presence of C. burnetii antibodies using an indirect ELISA. To identify potential risk factors for C. burnetii seropositivity, a multivariable mixed-effect logistic regression analysis was used. The study found an overall seroprevalence of 8.68% (95% CI: 6.11–11.25) and 13.57% (95% CI: 9.56–17.58) at the animal and herd levels, respectively, in the study areas. The results of the study indicated that C. burnetii infection was a widespread disease in the study areas. C. burnetii seropositivity at the animal level was significantly associated with age (OR = 4.1, 95%CI: 1.47–10.92), herd size (OR = 3.9, 95%CI: 1.21–12.66), management system (OR = 9.7, 95%CI: 1.27–27.25), cattle access to dogs, cats, and mice (OR = 2.5, 95%CI: 1.21–5.28), accessibility of cattle to wild animals (OR = 4.2, 95%CI: 1.01–17.18), presence of ticks on cattle (OR = 2.3, 95%CI: 1.12–4.83), and history of abortion (OR = 3.8, 95%CI: 1.78–8.23). A herd level analysis identified several risk factors for C. burnetii infection, including the management system (OR = 3.8, 95%CI: 1.59–8.98), agro-ecology (OR = 2.8, 95%CI: 1.43–7.21), herd size (OR = 4.3, 95%CI: 1.69–9.76), and accessibility of cattle to dogs, cats, and mice (OR = 2.6, 95%CI: 1.18–3.96). Therefore, it is important to implement appropriate control methods and raise public awareness about C. burnetii zoonotic transmission. Moreover, further studies should be conducted to isolate and characterize C. burnetii as a cause of reproductive problems and in disease reservoirs such as ticks and wildlife in the study areas.

Seroprevalence of Coxiella burnetii in sheep and goats in the Istrian Region, Croatia

A seroepidemiological study was conducted on the presence of Coxiella burnetii (C. burnetii) antibodies in sheep and goats in Istria, the largest peninsula in Croatia. Random blood samples were taken from 634 sheep and goats at different localities throughout the region. The aim of the study was to assess the prevalence of C. burnetii infection in sheep and goats, which represent the most important reservoir of infection in humans. C. burnetii antibody detection was performed by ELISA (LSIVet Ruminant Q Fever-Serum/Milk). Seroprevalence of C. burnetii was proven in 6.2% of sheep and 3.5% of goats. Larger herds, poor hygienic conditions on farms, a higher presence of goats in a restricted area, and the northern part of Istria proved to be significant risk factors for the seropositivity of animals.The southern part of Istria is known to have endemic Q fever, but no studies have been carried out so far to explore this issue. To gain a more complete epidemiological picture of Q fever in Istria, studies in humans, especially those professionally exposed to C. burnetii infection, should be performed.

Seroprevalence of Q Fever and Risk Factors Affecting Transmission of Coxiella burnetii in Industrial Slaughterhouse; A Survey from Northeastern Iran

Background: Q fever is a generally neglected infection caused by Coxiella burnetii. Slaughterhouse workers exposed to livestock are among occupationally at-risk people. Objectives: This study was conducted to investigate the seroprevalence of anti-Coxiella burnetii (Q fever) IgG antibody among industrial slaughterhouse workers and factors affecting the risk of infection. Methods: In this cross-sectional study serum samples were taken from 91 individuals working at the central industrial abattoir in Mashhad, Iran using a convenient sampling method. Sera were kept at -80°C until assayed for specific anti-Coxiella burnetii IgG antibodies (phase 1) using the commercial ELISA kit. The participants filled out a checklist addressing potential risk factors of acquiring the infection. SPSS 11.5 was used for data analysis considering a significance level of P < 0.05. Results: The participants’ mean age was 38.7 ± 8 years. Fifty-six percent of the studied individuals (51 out of 91) were found positive for anti-Coxiella burnetii antibodies. The most prevalent cases were sheep (29, 57%) and cow (18, 35%) butchers. The odds of Q fever infection increased among those with a history of accidental hand cuts of more than five times during the previous years (OR = 2.56, CI95% = 1.02 - 6.33, P-value = 0.04) and those dealing with sheep as the primary livestock (OR = 2.9, CI95% = 1.09 - 7.66, P = 0.02). Conclusions: The high seropositivity rate of anti-Coxiella burnetii IgG reflects high exposure rate of workers to this potentially serious pathogen in slaughtherhouses; therefore, careful education, follow-up, and revision of decontamination policies and improved occupational care and environmental hygiene should be strictly implemented in slaughterhouses to reduce the risk.

Coxiella burnetii and its risk factors in cattle in Egypt: a seroepidemiological survey

Animal production is greatly affected by Q fever. As a result of a lack of methodology and financial means to perform extensive epidemiological surveys, the disease's underdiagnosis has proven to be a challenge for effective control. The present study aimed to determine the seroprevalence of C. burnetii in cattle raising in four governorates situated at Nile Delta of Egypt and assess the associated risk factors for infection. A total of 480 serum samples were collected from cattle and examined for presence of anti-C. burnetii antibodies using indirect ELISA assay. The overall seroprevalence of C. burnetii among examined cattle was 19.8%, with the Qalyubia governorate having the highest prevalence. The results of multivariable logistic regression analysis revealed significant association between C. burnetii seropositivity and age, communal grazing and/or watering, contact with small ruminants and history of infertility. According to the findings of this work, C. burnetii is circulating among cattle living in Nile Delta. It is suggested that adequate hygiene procedures and biosecurity measures should be implemented to limit the transmission of pathogens within cow herds and potential human exposure.