Burley Tobacco Research Articles

Nontarget analysis of natural flavor metabolites in tobacco extracts

Large-scale production of tobacco generates significant amounts of waste, making the reuse and recycling of tobacco waste crucial for sustainability. However, the high diversity of tobacco metabolites requires a comprehensive analysis of tobacco waste extracts, so as to optimize extraction selectivity and recovery. In this study, we thoroughly analyzed the metabolic composition of 168 tobacco extracts using nontarget analysis. The extracts were derived from 56 tobacco wastes covering all four major tobacco types: flue-cured, dark air-cured, Burley, and Oriental tobaccos. From each sample, we obtained three types of extracts, such as concretes, absolutes, and supercritical fluid extraction (SFE) oils, through various extraction protocols. By matching deconvolved experimental MS/MS spectra with databases, 587 metabolites were putatively annotated, which were categorized into 24 groups, including alkaloids, indoles, and flavor compounds. The metabolic compositions of extracts from dark air-cured and Burley tobaccos were found to be similar, whereas flue-cured and Oriental tobaccos contained more diverse flavor components. Notably, concretes of various tobaccos prepared with 70 %-ethanol contained similar amounts of addictive alkaloids. However, the absolutes obtained by sequential extraction of these concretes with 95 %-ethanol effectively removed alkaloids while modestly maintaining the flavor compounds. Conversely, extraction protocols incorporating supercritical fluid extraction followed by 95 %-ethanol extraction resulted in the loss of most flavor compounds, likely due to the incompatibility between the solvents used in the two extraction steps. In conclusion, our study demonstrates that absolutes derived through secondary extraction with varying ethanol-water ratios holds significant potential for utilization.

Use of heterosis and transgression events to optimize sizes of leaves in variety group Burley tobacco

The manifestations of heterosis and transgression regarding the length and width of the leaves in ten hybrid combinations of Burley tobacco аre investigated. The results of the study show that in almost all variants and in both indicators, the values of the hypothetical heterosis exceed those of the real one. According to length of leaves heterosis with economic significance is observed only in one of the investigated hybrid combinations. Transgressive manifestations for this indicator are even less pronounced and are not significant in any of the studied options. In contrast to length of leaves the manifestations of heterosis are of economic importance in six of the ten crosses studied and could be successfully used to increase of width of leaves. Transgressive events in width of leaves are less pronounced than heterosis, but much more pronounced than those in the length. Direction of crossing affects the heterosis manifestations only of the length of the leaves but not those concerning their width. In terms to width of leaves, the manifestations of heterosis and transgression are related. Of all the studied variants, the one with the highest selection value regarding sizes of leaves is Hybrid 1555.

Integrative physiological and transcriptome analysis unravels the mechanism of low nitrogen use efficiency in burley tobacco.

Burley tobacco, a chlorophyll-deficient mutant with impaired nitrogen use efficiency (NUE), generally requires three to five times more nitrogen fertilization than flue-cured tobacco to achieve a comparable yield, which generates serious environmental pollution and negatively affects human health. Therefore, exploring the mechanisms underlying NUE is an effective measure to reduce environmental pollution and an essential direction for burley tobacco plant improvement. Physiological and genetic factors affecting tobacco NUE were identified using two tobacco genotypes with contrasting NUE in hydroponic experiments. Nitrogen use inefficient genotype (TN90) had lower nitrogen uptake and transport efficiencies, reduced leaf and root biomass, lower nitrogen assimilation and photosynthesis capacity, and lower nitrogen remobilization ability than the nitrogen use efficient genotype (K326). Transcriptomic analysis revealed that genes associated with photosynthesis, carbon fixation, and nitrogen metabolism are implicated in NUE. Three nitrate transporter genes in the leaves (NPF2.11, NPF2.13, and NPF3.1) and three nitrate transporter genes (NPF6.3, NRT2.1, and NRT2.4) in roots were down-regulated by nitrogen starvation, all of which showed lower expression in TN90 than in K326. In addition, the protein-protein interaction (PPI) network diagram identified eight key genes (TPIP1, GAPB, HEMB, PGK3, PSBO, PSBP2, PSAG, and GLN2) that may affect NUE. Less advantageous changes in nitrogen uptake, nitrogen assimilation in combination with nitrogen remobilization, and maintenance of photosynthesis in response to nitrogen deficiency led to a lower NUE in TN90. The key genes (TPIP1, GAPB, PGK3, PSBO, PSBP2, PSAG, and GLN2) were associated with improving photosynthesis and nitrogen metabolism in tobacco plants grown under N-deficient conditions.

Биологични и стопански показатели на хибриди в първо поколение тютюн Бърлей

An assessment is made the biological and economical chemical indicators of 11 samples Burley tobacco, which 10 new promising hydrides in first generation and standard Pliska 2002 variety. As a result, the study found that the most favorable biometric indicators is presented Hybrid 1595A. With the short vegetative period is characterized Hybrid 1594, and the longest such – standard Pliska 2002 variety. All new selection lines excel at length of the vegetative period standard Pliska 2002 variety. The highest yield is represented by Hybrid 1595A, followed by Hybrid 1593. The highest percentage of first grade is derived from Hybrid 1593А, which gives the lowest percentage of third grade. With favorable ratio of grades is formed and Hybrid 1593. All new lines superior the standard variety Pliska 2002, to as yield, so and percentage of first grade. The studied hybrids, according to their biometric indicators, are grouped into two clusters, and two main components influence the clustering of the hybrids and explain 77.17% of the total variation. Depending on their economic indicators, the hybrids are arranged in two clusters, and in them, too, two main components are the cause of 89.27% of the total variation. Complex assessment of biological and economic indicators shows that with the best parameters differs Hybrid 1593, and work with it should continue with production testing. All new lines superior the standard variety Pliska 2002, to as yield, so and percentage of first grade, which is an indicator of the success of the selection work.

Pyrolysis characteristics of different types of tobacco and its correlation with the released aroma components under heating condition

An in-depth insight into the pyrolysis characteristics of tobacco is of great significance for the utilization of tobacco wastes. The effects of glycerol addition on the pyrolysis characteristics and distribution of aroma components of four types of tobacco was investigated in this study by using thermogravimetry and self-built rapid heating furnace capture and GC-MS analysis. Further, correlation network analysis was used to study the correlation between pyrolysis characteristics and released aroma components. It was found that the thermal weight loss behavior of different samples exhibited both commonalities and differences, the thermal weight loss process could be divided into four stages. After the application of glycerol, the overall performance of all types of tobacco leaves at stage II showed that DTGmax2 and WL2 increased, and glycerol had a relatively obvious effect on the pyrolysis characteristic parameters of flue-cured tobacco. Pyrolysis kinetic analysis based on Coats-Redfern method showed that the activation energy and pre-exponential factor of tobacco samples were decreased after the addition of glycerol. Under the heating condition, the total amount of released aroma components from each type of tobacco followed the order of burley tobacco > flue-cured tobacco >oriental tobacco > yellow sun-cured tobacco. The release amount of heterocyclic and olefins compounds from burley tobacco were higher, while flue-cured tobacco released higher contents of esters, ketones and furans compounds. The addition of glycerol promoted the release of aroma components. In terms of correlation, Tmax2 was negatively correlated with esters, aldehydes, and furans compounds, and positively correlated with DTGmax3. Tmax3 was significantly correlated with a lot of indicators, positively with acids, aldehydes, and furans compounds, but negatively with olefins and heterocyclic compounds.

Sensitivity of Phytophthora nicotianae in Tennessee and North Carolina to Mefenoxam, Oxathiapiprolin, Mandipropamid, and Fluopicolide.

Phytophthora nicotianae causes devastating disease in a range of hosts, including tobacco (N. tabacum L.), tomato, citrus, strawberry, and numerous ornamentals. Black shank, caused by P. nicotianae, is the most economically important disease to tobacco production in Tennessee and North Carolina. Black shank management includes the use of resistant cultivars, crop rotation, and fungicides. Fungicide resistance is a concern for black shank management due to the limited number of active ingredients available and the repeated exposure of pathogen populations to these products. In vitro fungicide sensitivity assays were conducted on 155 P. nicotianae isolates collected in Tennessee and North Carolina in 2021 and 2022 to determine their EC50 values for oxathiapiprolin, mandipropamid, and fluopicolide. The P. nicotianae was isolated predominantly from burley, dark, and flue-cured tobacco showing symptoms of black shank as well as tomato with buckeye rot symptoms. A discriminatory dose was used to determine each isolate's sensitivity to mefenoxam in 2021 and 2022. In 2021, EC50 values were determined for oxathiapiprolin, mandipropamid, and fluopicolide. In 2022, discriminatory doses based on EC75 values were used to determine each isolate's sensitivity to these fungicides. All isolates from the 2 years were sensitive to mefenoxam, mandipropamid, and fluopicolide. One isolate in 2022 was moderately sensitive to oxathiapiprolin, while all other isolates were sensitive.

Кoнкурсно изпитване между местни и интродуцирани сортове тютюн Бърлей

During the period 2016-2020, is made in the experimental field of TTРI a comparative complex evaluation of six samples of tobacco from the Burley varietal group, of which three introduced varieties and the same number of indigenous varieties,. The subject of the study are biometric, biological, economic and chemical indicators of the studied variants. As a result of the study, it is found that the researched genotypes presented with favorable Burley tobacco biometric indicators. All studied varieties have a shorter vegetative period than the standard Pliska 2002 variety. The highest yield is presented by Burley 1000 variety, followed by a small but proven difference by Burley 1317 variety. The highest percentage of the first grade is distinguished by the Tennessee 90 variety, which also gives the lowest percentage of the third grade. Tennessee 90 variety also has the most favorable chemical composition in terms of all the investigated chemical indicators.The complex assessment of the biological, economic and chemical indicators shows that it is not possible to distinguish a variety that is superior to the other tested variants. In terms of percentage of grades and chemical composition, the introduced variants are superior to the local varieties, but they are seriously inferior to them in terms of yield. The Tennessee 90 and Tennessee 86 varieties have similar biometric, economic and chemical characteristics with a slight predominance of Tennessee 90. Tennessee 90 variety namely has the highest selection value of the introduced varieties. The studied introduced varieties are valuable genetic material that can be successfully used in the selection of Burley tobacco.

Comparative Technological Assessment of the Quality Formation of Local and Introduced Varieties of Virginia Flue-cured and Burley Tobaccos

The production of “marketable” tobacco is decisive in the pReferences of local farmers towards certain varieties of Virginia and Burley tobaccos. The aim of the study was to complete a comparative technological evaluation of the quality potential of introduced and local varieties of Virginia flue-cured and Burley air-cured tobaccos in Bulgaria. The investigation was conducted with tobaccos from 2018 crop year and included 4 varieties of Virginia flue-cured and 2 varieties of Burley tobacco (local and introduced), grown in different regions of Southern and Northern Bulgaria. The complex assessment of tobacco quality was based on leaf and smoke chemical indicators, external leaf quality elements and smoking performance of the studied tobaccos. The final rating was achieved by referring to the value of the calculated “quality indices”. Two of the local varieties, V 0454 from region Parvomay and PVH 19 from Central Northern Bulgaria were rated the best in the complex evaluation of the FCV tobaccos. A better complex expression of quality was found in the introduced (trial) variety Burley N compared to the local Burley 1317 in Southern Bulgaria region.

Single-cell transcriptome analyses reveal cellular and molecular responses to low nitrogen in burley tobacco leaves.

Tobacco (Nicotiana tabacum) is cultivated and consumed worldwide. It requires great amounts of nitrogen (N) to achieve the best yield and quality. With a view to sustainable and environmentally friendly agriculture, developing new genotypes with high productivity under low N conditions is an important approach. It is unclear how genes in tobacco are expressed at the cellular level and the precise mechanisms by which cells respond to environmental stress, especially in the case of low N. Here, we characterized the transcriptomes in tobacco leaves grown in normal and low-N conditions by performing scRNA-seq. We identified 10 cell types with 17 transcriptionally distinct cell clusters with the assistance of marker genes and constructed the first single-cell atlas of tobacco leaves. Distinct gene expression patterns of cell clusters were observed under low-N conditions, and the mesophyll cells were the most important responsive cell type and displayed heterogene responses among its three subtypes. Pseudo-time trajectory analysis revealed low-N stress decelerates the differentiation towards mesophyll cells. In combination with scRNA-seq, WGCNA, and bulk RNA-seq results, we found that genes involved in porphyrin metabolism, nitrogen metabolism, carbon fixation, photosynthesis, and photosynthesis-antenna pathway play an essential role in response to low N. Moreover, we identified COL16, GATA24, MYB73, and GLK1 as key TFs in the regulation of N-responsive genes. Collectively, our findings are the first observation of the cellular and molecular responses of tobacco leaves under low N stress and lay the cornerstone for future tobacco scRNA-seq investigations.

Hybridological analysis of inheritance of length and width of leaves in hybrid combinations Burley tobacco

The way of inheritance, the coefficient of heritability, the manifestations of heterosis, transgression and depression in terms of the length and width of the leaves in hybrid combinations Burley tobaco are established. For that purpose are studied, the Р1 , Р2 , F1 and F2 populations of eight crosses Burley tobacco. The obtained results suggest that inheritance of the length or leaves is overrdominantly, incompletely dominantly or additive, and of the width of leaves is incompletely dominantly and overrdominantly,, as in both indicators it is always in the direction of the parent with higher values. Manifestations of heterosis and transgression are of economic importance only in terms of leaf width. The minimal number of genes that control the expression of the studied indices is small and uniform and the length and width of the leaves. The phenotypic expression of leaf size is strongly influenced by negative epistatic interactions, especially pronounced at their width. The established medium to higher values of the coefficient of heritability of the length of the leaves are an indication of a relatively equal share of influence of genotype and environmental conditions on the phenotypic manifestation of leaf length, which suggests that the selection on this indicator to be effective in earlier hybrid generations. The selection of width of the leaves will be effective in later hybrid generations due to lower values coefficient of the heritability, for this indicator. This shows that the coefficient of heritability and the related coefficient of efficiency of the selection is different in both indicators, making it difficult selection work.

Pepper vein mottling virus (PVMV) – нов вирус по тютюна в България

The aim of the present study is to trace the emergence and development of TEV and PVMV, new viruses for tobacco in Bulgaria, in the three varietal groups of Burley, Virginia and Basmi tobacco grown in the central part of southern Bulgaria. During the period 2017-2019, immunological studies were carried out to prove new viruses for tobacco in Bulgaria from the group of potyviruses PVMV and TEV. A kit for serological identification of the French company SEDIAG S.A.S. was used to prove the viruses. To detect TEV, TAS-ELISA was performed with IgG dilution of 1:100 and DAS-ELISA - to detect PVMV with IgG dilution of 1:100. In Virginia and Basmi tobacco variety groups, the potyvirus PVMV was serologically proven, and the virus was identified for the first time in tobacco in Bulgaria. PVMV was demonstrated in monoinfection and in complex with TEV in isolates from the cultivar group Virginia and Basmi. TEV and PVMV were not detected in isolates from the Burley cultivar group. In tobacco, the two potyviruses cannot be distinguished based on symptoms alone. Accurate diagnosis is possible by applying an ELISA or PCR test

Reducing nitrate and tobacco-specific nitrosamine level in burley tobacco leaves through grafting on flue-cured tobacco rootstock.

Nitrosation of pyridine alkaloids in tobacco generates tobacco-specific nitrosamines (TSNAs), which are notable toxicants in tobacco products and smoke. Burley tobacco, a chloroplast- and nitrogen (N)-deficient phenotype that accumulates high levels of nitrate-nitrogen (NO3-N) in its leaves, is particularly susceptible to TSNAs formation. In this study, reciprocal pot and field grafting experiments were conducted using burley tobacco Eyan No.1 and flue-cured tobacco K326 to investigate whether grafting burley tobacco scions on flue-cured tobacco rootstocks could enhance pigment biosynthesis and photosynthesis, while reducing the NO3-N level in burley tobacco leaves. Grafting burley tobacco scions on flue-cured tobacco rootstocks significantly increased the total pigment content, photosynthetic rate, biomass, nitrate reductase and glutamine synthetase activities, as well as ammonium-nitrogen (NH4-N), total soluble and reducing sugar, and soluble protein levels in burley tobacco leaves compared with burley tobacco self-rooting, while decreasing the NO3-N level and nitrate-N to total N ratio. Transcriptomic analysis revealed that grafting resulted in upregulated expression of genes involved in starch, sucrose, porphyrin, chlorophyll, and N metabolism, as well as carbon fixation and carotenoid biosynthesis. The findings suggest that grafting on high N use efficiency rootstock is an exceptionally promising means of decreasing NO3-N accumulation by improving photosynthesis and N metabolism in the scion, thereby reducing the levels of harmful TSNAs.

Modified physiology of burley tobacco plants genetically engineered to express Yb1, a functional EGY enzyme.

Transgenic overexpression of a NtEGY2 gene restores normal green color of burley tobacco plants, but does not increase nitrogen utilization efficiency beyond that exhibited by wild-type individuals. Nitrogen physiology is important in tobacco because of its role in generation of leaf yield and accumulation of nitrogen-containing alkaloids that can react with nitrosating agents in the formation of carcinogenic tobacco-specific nitrosamines. Cultivars of the burley tobacco market class are homozygous for deleterious mutant alleles at the duplicate Yb1 and Yb2 loci which have previously been associated with decreased nitrogen use and utilization efficiency; increased leaf nitrate, total nitrogen, and alkaloid levels; and reduced yields. How mutant alleles at these two loci affect these traits is not well understood. Recent characterization of the Yb1 and Yb2 genes (homologs of Arabidopsis EGY1 gene) enabled overexpression of the wild-type Yb1 allele in yb1yb1yb2yb2 plants to determine if observed unfavorable effects were due to linkage or pleiotropy, and to determine if overexpression could lead to beneficial modifications in any of these traits in transgenic plants relative to naturally-occurring wild-type genotypes. Yb1 overexpression was found to confer an agronomic benefit to yb1yb1yb2yb2 genotypes but no advantage to wild-type genotypes. RNA-Seq was used to carry out a comparative transcriptome analysis of genetically engineered and wild-type nearly isogenic lines (NILs) to gain insight on metabolic pathways affecting carbon and nitrogen metabolism that might be altered as the result of genetic variability at the Yb1 and Yb2 loci. Results indicate that complex changes in the transcriptome of tobacco can be manifested by altered expression of Yb1.

Differential oxidative stress homeostasis in two burley tobacco varieties is linked to different peroxisomal glycolate oxidase levels

Previous analyses of burley tobacco varieties KT 204LC and NCBH 129LC revealed that NCBH 129LC has a higher oxidative stress tolerance level and accumulates more hydrogen peroxide. We conducted untargeted metabolomics analyses to deepen our understanding of these varietal differences in redox status, and here, we show that KT 204LC and NCBH 129LC significantly differ in lipid metabolism, with NCBH 129LC containing more lipids than KT 204LC. Considering that NCBH 129LC also has more hydrogen peroxide and that peroxisomes play a major role in hydrogen peroxide production and lipid metabolism, we next analyzed peroxisomal markers. Glycolate oxidase, the key photorespiratory enzyme and a significant producer of hydrogen peroxide, accumulated significantly higher in NCBH 129LC. Our data suggest that differences in the oxidative stress status of burley varieties KT 204LC and NCBH 129LC are – at least partially - caused by different peroxisomal activities of the two varieties.

Energy and emission properties of burley tobacco stalk briquettes and its combinations with other biomass as promising replacement for coal.

As a tobacco producer, Serbia has to deal with large amounts of leftover tobacco stalks after harvesting. One option for this type of biomass is to burn it, but burning is not encouraged in Serbia, since the levels of its combustion products have not been investigated yet. The aim of this study was therefore to determine the elemental composition, ash and nicotine content, heat values, and composition of gaseous combustion products of tobacco stalk briquettes and to see if their mixing with other types of biomass available in Serbia could improve their ecological profile. We made 11 different types of briquettes: six of pure raw materials, including burley tobacco stalks, sunflower head remains, wheat straw, corncob, soy straw, and beech sawdust and five by mixing tobacco stalks with these other raw materials in a 50:50 mass ratio. All briquettes meet the ecological criteria regarding the emission limits for nitrogen oxides (NOx), sulphur dioxide, carbon monoxide, and carbon dioxide. Nicotine content in flue gases (<10 mg/kg) is far below the maximum level allowed by the European Union. Heat values of all biomass samples are acceptable, although lower than those specified for solid biofuels (≥16.0 MJ/kg), save for corncob and beech sawdust and their mixtures with tobacco stalks. Our findings therefore encourage the use of tobacco stalks as a viable biofuel.

Green Yield as a Potential Predictor of Cured Burley Tobacco Yield in a Phytophthora nicotianae-Infested Field over Four Seasons

Phytophthora-induced plant diseases are most effectively managed using an integrated strategy that includes host resistance, best cultural practices, and fungicide applications. Black shank, caused by Phytophthora nicotianae, is the most significant soilborne disease affecting tobacco, and extensive breeding for resistance has resulted in an array of varieties with a spectrum of black shank resistance. Estimates of plant survival are the most common measurement in black shank variety and disease management trials; however, survival might not be the most accurate indicator of yield for varieties with robust resistance. Through the presented analyses, at-harvest green yield estimates are shown to be more closely associated with cured tobacco yields than survival counts, irrespective of black shank resistance status. However, under high black shank pressure, green yield is an excellent descriptor of only low-resistance varieties’ cured yield, with other factors likely contributing to moderate- and high-resistance varieties’ cured yield models. This prevents development of a unified (variety-nonspecific) cured yield model, which would be useful not only for tobacco scientists but also commercial growers in planning labor activities and the industry at large in managing cured tobacco inventory. More broadly, this suggests that research in other Phytophthora pathosystems may benefit from preliminary yield estimates in place of or in addition to survival counts in resistant variety evaluations.

Understanding the Effects of Infill Patterns and Fiber Loading on Mechanical Properties of 3D Printed Tobacco Stalks-PLA Composites Using X-Ray Tomography

This study utilized the Philippine burley and native tobacco as reinforcing materials to Polylactic Acid (PLA) in creating novel natural fiber-thermoplastic 3D printing filaments. As a new material for 3D printing, the effects of fiber loading, fiber species, and printing pattern on the mechanical properties of the tobacco fiber-PLA composites were investigated. Regardless of tobacco species and fiber loading, the honeycomb pattern showed superior tensile strength than its rectilinear counterpart. The same trend was also observed in the impact strength of some tobacco-PLA samples. Increase in fiber loading on the other hand, decreased the flexural strength and flexural modulus of the tobacco fiber-PLA composites. The trends in the mechanical properties were then analyzed using 3D X-Ray Computed Tomography, which allowed analysis and visualization of voids and layer patterns.

Management of Pythium myriotylum in burley tobacco transplants and its effect on field productivity

Pythium root rot (PRR) is a significant disease of tobacco transplants produced in the greenhouse floatbed system, but little is known about its influence once PRR-infected plants are transplanted in the field. Tobacco producers have limited chemical options to manage PRR, and additional data are needed on the efficacy of the fungicide active ingredients etridiazole, mefenoxam, and hydrogen peroxide-peroxyacetic acid. With the support of a pictorial, ordinal root ball quality (RBQ) rating scale, etridiazole and mefenoxam improved RBQ in tobacco transplants inoculated with Pythium myriotylum in greenhouse trials. In multi-year, generalized linear mixed model analyses, higher average RBQ in transplants significantly increased plant establishment, survival to season-end, and cured yield in research farm trials. To further apply the pictorial scale, a potential threshold of average RBQ >3.0 would be expected to result in 201 kg/ha more cured yield, over transplants with average RBQ ≤3.0. These results document for the first time the influence of transplant PRR on cured tobacco yield, in addition to its interim effects on tobacco establishment and survival. While additional data are needed to support a critical RBQ value, estimated effects of transplant quality on cured tobacco yield may assist growers in post-transplant farm management decisions, such as labor planning.

Effects of different tobacco materials on the release characteristics of aroma components

In order to reveal the release characteristics of different tobacco materials, the characteristics of tobacco materials and the release characteristics of smoke were analyzed. This paper studied 17 kinds of tobacco materials, the thermogravimetric analyzer was used to study the thermogravimetric characteristics of the tobacco materials, meanwhile the gas chromatography was used to analysis original aroma composition characteristics of the raw tobacco material, the smoke released from the pyrolysis of tobacco in a fixed bed reactor was also studied. The results showed: ① The elemental composition of different kinds of tobacco materials was similar, but there were significant differences in the volatile content (aroma components), among which the aroma components of tobacco stem were the highest. ②Different categories of tobacco raw materials have different properties of the aroma components. Among them, the nicotine content of roasted tobacco was higher, spice tobacco and tobacco stems mainly contain acid and ketone aroma components. However, burley tobacco and cigar tobacco are distinguished from other types of tobacco by the presence of aromatic substances such as new phytodiene.③ Through the analysis of thermal release characteristics of aroma components of different tobacco raw materials, it was found that the yield of aroma components was positively correlated with the volatile content of tobacco materials. The results of this study clarified the effects of different tobacco raw materials on the release characteristics of aroma components and provided theoretical guidance for the subsequent compound utilization of tobacco.

Effects of different tobacco materials on the release characteristics of aroma components

In order to reveal the release characteristics of different tobacco materials, the characteristics of tobacco materials and the release characteristics of smoke were analyzed. This paper studied 17 kinds of tobacco materials, the thermogravimetric analyzer was used to study the thermogravimetric characteristics of the tobacco materials, meanwhile the gas chromatography was used to analysis original aroma composition characteristics of the raw tobacco material, the smoke released from the pyrolysis of tobacco in a fixed bed reactor was also studied. The results showed: ① The elemental composition of different kinds of tobacco materials was similar, but there were significant differences in the volatile content (aroma components), among which the aroma components of tobacco stem were the highest. ②Different categories of tobacco raw materials have different properties of the aroma components. Among them, the nicotine content of roasted tobacco was higher, spice tobacco and tobacco stems mainly contain acid and ketone aroma components. However, burley tobacco and cigar tobacco are distinguished from other types of tobacco by the presence of aromatic substances such as new phytodiene.③ Through the analysis of thermal release characteristics of aroma components of different tobacco raw materials, it was found that the yield of aroma components was positively correlated with the volatile content of tobacco materials. The results of this study clarified the effects of different tobacco raw materials on the release characteristics of aroma components and provided theoretical guidance for the subsequent compound utilization of tobacco.