Investment in the food cold chain has been reportedly identified as a solution to extensive postharvest losses worldwide. However, cold chain investment in the Ugandan food value chain is largely unknown. This study located, characterised, and determined the efficacy of bulk cold storage facilities used in handling perishable agricultural food produce in the 16 city districts of Uganda. Only 9/16 city districts had bulk cold storage facilities, translating to a cold storage capacity of 4.5 m3 per 1,000 inhabitants. The private sector owned the highest proportion by volume (90.3%) of these facilities with the highest volume used for handling multiple perishable products (11,590 m3). Generally, temperature varied significantly from the respective set points in the facilities. Variations were observed for between 1/3 to 3/4 of the logged period above maximum allowable storage temperatures for the different products, showing efficacy lapses. These results indicate investment and management gaps and opportunities in this indelible sector in food value chain by both government and private sector players.