Abstract In 2005 and 2007, prompted by Nipah and avian influenza outbreaks, respectively, faculties at Chattogram Veterinary and Animal Sciences University (CVASU) in Bangladesh initiated a civil society network of researchers in animal and human health. This network led to the launch a year later of One Health Bangladesh (OHB), a platform with membership open to multi-disciplinary scientists, practitioners, and activists. Along with continued advocacy to advance One Health agenda, OHB partnered with government, UN agencies, and other stakeholders to develop, in 2012, a National One Health Strategic Framework and Action Plan. The National Plan was later endorsed by the Ministry of Health and Family Welfare (MoHFW), Ministry of Fisheries and Livestock (MoFL), and Ministry of Environment, Forest, and Climate Change (MoEFC) followed by its implementation which introduced a three-tier multi-sectoral coordination mechanism including the establishment of a One Heath Secretariat in 2016. In 2017, an operational budget for the One Health Secretariat (OHS) was allocated by the MoHFW and three mid-level government officers from three government agencies, and the Directorate General of Health Services (DGHS), Department of Livestock Services (DLS), and Bangladesh Forest Department (BFD) were seconded to the OHS to make the Secretariat functional. OHB and the OHS now work together to advance the institutionalization process and implement One Health activities including One Health capacity building, joint surveillance, outbreak investigation and intervention, advocacy in the form of arranging dialogs, seminars, workshops, and conferences, and promoting cross sectoral and transdisciplinary collaboration, networking, and partnership nationally and internationally. Information © The Authors 2024