Buccal Glands Research Articles

Capybara (Hydrochoerus hydrochaeris) Salivary Glands Morphology.

Morphological studies concerning salivary glands have emerged as an exciting tool to understand feeding habits. In this sense, this study aimed to describe capybara salivary glands morphology to understand potential morphological associations to this species feeding habits. Macroscopic dissections and microscopic analyses were performed on eight specimens. The findings indicate that capybaras have three pairs of major (parotid, mandibular and sublingual) and two pairs of minors (dorsal and ventral buccal) salivary glands. The parotid gland, the largest, is irregularly shaped, forming small macroscopic lobes. The mandibular gland is rounded and found syntopically alongside the mandible angle and arranged medially to the ventral projection of the parotid gland. The sublingual gland is a compact elongated and semicircular mass. The dorsal and ventral buccal glands are located in the buccal portion of the buccinator muscle. Histologically, the parotid and mandibular glands are composed of serous acini, while the sublingual and buccal (dorsal and ventral) glands consist of mucous tubules containing serous demi-lunes at their periphery. Capybara salivary glands reveal morphological associations with a predominantly herbivorous diet. The parotid gland, for example, notably developed in size and volume, suggests a specialisation for increased saliva production, necessary for chewing, forming the food bolus, swallowing fibrous foods, and protecting the oral cavity ecosystem.

New record of Joffre's pipistrelle, Mirostrellusjoffrei (Chiroptera, Vespertilionidae), in China.

Mirostrellusjoffrei Thomas, 1915 is the sole species within the genus Mirostrellus and its occurrence is notably rare in China. Despite the recent discovery of four previously unreported specimens from western Yunnan, China (Zaoteng River, 25.31°N, 98.80°E, altitude 1451 m) at the National Museum of Prague in the Czech Republic, comprehensive descriptions or detailed accounts of this species within China have yet to be published. During a field expedition within the Guanyinshan Provincial Nature Reserve, Yuanyang, Yunnan, China, a bat was captured using a mist net. The specimen was of moderate size, with its ventral surface presenting a golden-brown hue and its dorsal surface ranging from dark brown to obsidian. The forearm measured 37.31 mm, while the fifth digit was conspicuously diminished in comparison to the others. The snout was robust, featuring prominent buccal glands. It was characterised by a well-developed upper frame and a barely visible low sagittal crest. The upper canines possessed well-developed posterior cusps. The dentition comprised two upper and two lower premolars, with the first upper premolar being notably small and the lower molars being distinctly myotodont. These attributes correspond with the known traits of Mirostrellusjoffrei. Phylogenetically, the sequence of this specimen clustered with that of M.joffrei, forming an independent clade. Through an examination of its morphological characteristics and a phylogenetic analysis of the mitochondrial cytochrome b (Cyt b), cytochrome oxidase subunit I (COI) and recombination activating gene 2 (RAG2) sequences, we identified the specimen as M.joffrei, thus confirming the presence of Joffre's pipistrelle in China.

Identification of antibacterial activity of liver-expressed antimicrobial peptide 2 (LEAP2) from primitive vertebrate lamprey

Liver-expressed antimicrobial peptide 2 (LEAP2) is a member of the antimicrobial peptides family and plays a key role in the innate immune system of organisms. LEAP2 orthologs have been identified from a variety of fish species, however, its function in primitive vertebrates has not been clarified. In this study, we cloned and identified Lc-LEAP2 from the primitive jawless vertebrate lamprey (Lethenteron camtschaticum) which includes a 25 amino acids signal peptide and a mature peptide of 47 amino acids. Although sequence similarity was low compared to other species, the mature Lc-LEAP2 possesses four conserved cysteine residues, forming a core structure with two disulfide bonds between the cysteine residues in the relative 1–3 (Cys 58 and Cys 69) and 2–4 (Cys 64 and Cys 74) positions. Lc-LEAP2 was most abundantly expressed in the muscle, supraneural body and buccal gland of lamprey, and was significantly upregulated during LPS and Poly I:C stimulations. The mature peptide was synthesized and characterized for its antibacterial activity against different bacteria. Lc-LEAP2 possessed inhibition of a wide range of bacteria with a dose-dependence, disrupting the integrity of bacterial cell membranes and binding to bacterial genomic DNA, although its inhibitory function is weak compared to that of higher vertebrates. These data suggest that Lc-LEAP2 plays an important role in the innate immunity of lamprey and is of great value in improving resistance to pathogens. In addition, the antimicrobial mechanism of LEAP2 has been highly conserved since its emergence in primitive vertebrates.

Spatial Metabolomics Reveals the Multifaceted Nature of Lamprey Buccal Gland and Its Diverse Mechanisms for Blood-Feeding

Lampreys are blood-sucking vampires in marine environments. From a survival perspective, it is expected that the lamprey buccal gland exhibits a repository of pharmacologically active components to modulate the host’s homeostasis, inflammatory and immune responses. By analyzing the metabolic profiles of 14 different lamprey tissues, we show that two groups of metabolites in the buccal gland of lampreys, prostaglandins and the kynurenine pathway metabolites, can be injected into the host fish to assist lamprey blood feeding. Prostaglandins are well-known blood-sucking-associated metabolites that act as vasodilators and anticoagulants to maintain vascular homeostasis and are involved in inflammatory responses. The vasomotor reactivity test on catfish aortic ring showed that kynurenine can also relax the blood vessels of the host fish, thus improving the blood flow of the host fish at the bite site. Finally, a lamprey spatial metabolomics database (https://www.lampreydb.com) was constructed to assist studies using lampreys as animal model.

Peptidomics Analysis Reveals the Buccal Gland of Jawless Vertebrate Lamprey as a Source of Multiple Bioactive Peptides.

Various proteins with antibacterial, anticoagulant, and anti-inflammatory properties have been identified in the buccal glands of jawless blood-sucking vertebrate lampreys. However, studies on endogenous peptides in the buccal gland of lampreys are limited. In this study, 4528 endogenous peptides were identified from 1224 precursor proteins using peptidomics and screened for bioactivity in the buccal glands of the lamprey, Lethenteron camtschaticum. We synthesized four candidate bioactive peptides (VSLNLPYSVVRGEQFVVQA, DIPVPEVPILE, VVQLPPVVLGTFG, and VPPPPLVLPPASVK), calculated their secondary structures, and validated their bioactivity. The results showed that the peptide VSLNLPYSVVRGEQFVVQA possessed anti-inflammatory activity, which significantly increased the expression of anti-inflammatory factors and decreased the expression of inflammatory factors in THP-1 cells. The peptide VVQLPPVVLGTFG showed antibacterial activity against some gram-positive bacteria. The peptide VSLNLPYSVVRGEQFVQA possessed good ACE inhibitory activity at low concentrations, but no dose-related correlation was observed. Our study revealed that the buccal glands of the jawless vertebrate lamprey are a source of multiple bioactive peptides, which will provide new insights into the blood-sucking mechanism of lamprey.

Light and Electron Microscopy of Buccal Salivary Glands of the Dromedary Camel

The study was aimed to investigate the light and electron microscopic structure of buccal salivary glands in the dromedary (Camelus dromedarius). Anatomically, the dromedary camel buccal glands were well-developed and were located on the lateral side of the cheek. Three parts (groups) of the buccal glands could be observed: dorsal, middle and ventral parts. Histologically, the secretory units were tubule-acinar, surrounded by myo-epithelial cells and supported by connective tissue. Whereas, the dorsal glands were purely mucous and the ventral glands were purely serous in secretion, the middle ones were muco-serous with predominant mucous cells. Ultra-structurally, the glandular cells of the serous secretory units (type I cells) contained numerous microvilli, many electron-dense secretory granules, abundant rough endoplasmic reticulum and mitochondria. On the other hand, the mucous-secreting cells (type II cells) appeared with numerous electron-translucent supra-nuclear granules and flat basal nuclei; dilated tubules of rough endoplasmic reticulum; fewer mitochondria and Golgi bodies were also observed. The obtained results have been compared with the previous reports on salivary glands of camel and other mammalian species.

A Single Firm Nodule of the Upper Lip: A Case Report

Canalicular Adenoma (CA) is a benign accessory salivary gland neoplasm. It represents less than 1% of salivary gland tumors. It occurs generally on the upper lips in female patients over 50 years of age. We report a case of canalicular adenoma that arose from buccal minor salivary glands of the upper lip without any symptomatology. Clinically, CA appears as an often single firm mass of 1cm, sparing the main salivary glands which is not pathognomonic of canalicular adenoma. Treatment of choice is enucleation to rule out many differential diagnoses that may present clinically as an asymptomatic nodule. Recurrences are rare. Histological features are characteristic and usually allow a definitive diagnosis to be made with confidence. However, many differential diagnoses remain to be ruled out.

A Single Firm Nodule of the Upper Lip: A Case Report

Canalicular Adenoma (CA) is a benign accessory salivary gland neoplasm. It represents less than 1% of salivary gland tumors. It occurs generally on the upper lips in female patients over 50 years of age. We report a case of canalicular adenoma that arose from buccal minor salivary glands of the upper lip without any symptomatology. Clinically, CA appears as an often single firm mass of 1cm, sparing the main salivary glands which is not pathognomonic of canalicular adenoma. Treatment of choice is enucleation to rule out many differential diagnoses that may present clinically as an asymptomatic nodule. Recurrences are rare. Histological features are characteristic and usually allow a definitive diagnosis to be made with confidence. However, many differential diagnoses remain to be ruled out.

Morphology of the lips, cheeks, and the hard palate of the Egyptian water buffalo (Bubalus bubalis): a focus on histological, histochemical, and ultrastructural aspects.

The available data is scanty about Egyptian water buffalo lips, cheeks, and palate. The current investigation was focused on describing the morphology of the lip, cheek, and palate. Our study included the gross, light, and electron microscopic examinations of ten heads of the Egyptian water buffalos. The nasolabial plate surface carried numerous scales of keratinized epithelium. Internal labial surface and labial mucocutaneous junctions were covered with stratified squamous keratinized epithelium. Two types of hair follicles in the dermis included ordinary and cavernous types characterized by cavernous space. The conical papillae on the internal aspect of the oral commissure were projected from the mucous membrane. Seromucous glands were occasionally observed under the oral mucous membrane of the commissure and gave positive PAS and AB. Conical papillae density on the inner cheek surface had some variations: the rostral part had large papillae, while the dorsal part had numerous papillae than the ventral part, the caudal part had a smaller number of papillae, while the middle part was devoid of papillae. Parotid duct opening in the buccal vestibule was without papillae. Conical papillae had two surfaces; the rostral surface was highly keratinized than the caudal one. The buccal gland was a compound tubuloacinar mixed (mucoserous) gland and mucus acini only reacted to PAS and AB. The oral surface of palatine rugae was covered with highly keratinized epithelium than the aboral surface. Palatine glands showed PAS and AB positive. The result describes the relationship between the available food particles, environmental conditions and the lip, cheek, and palate appearance, and structure.

Morphological Analysis of Angiotensin-Converting Enzyme 2 Expression in the Salivary Glands and Associated Tissues

We evaluated localization of angiotensin-converting enzyme 2 (ACE2), the receptor for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), in the salivary and associated tissues using immunohistochemistry. Fifty paraffin-embedded blocks from 48 anonymized patients, biopsied or operated on for diseases of the oral and maxillofacial region before 2010, were analyzed. ACE2-expressing cells were observed in the parotid, sublingual and the buccal glands, the conduits, the acinar regions of the serous glands, and sparsely in the mucous glands. Scattered ACE2-positive endothelial cells were also observed in nearby capillaries nourishing the salivary glands, as well as in the juxta-epithelial capillaries of the oral mucosa. ACE-2-positive adipocytes were scattered within the stroma of the parotid gland. These observations suggest the possibility that SARS-CoV-2 may travel through the bloodstream to the capillaries that nourish the salivary glands and oral mucosa, and inducing vasculitis and damage of oral tissues. SARS-CoV-2 infection of salivary glands through the bloodstream implies the main cause of salivary contamination. Similarly, ascending infection from oral fluid to the salivary gland conduit has been shown to be another possible mute. Moreover, infection of ACE2-positive parotid adipocytes may lead to parotid glands inflammation and contribute to systemic progression of coronavirus disease 2019.

Juvenile pleomorphic adenoma of the cheek: Report of a rare case and literature analysis

Abstract Purpose The aim of this paper is to describe a rare case of children with pleomorphic adenoma in buccal minor salivary gland and present a brief review of the previous cases in the literature. Methods Research was conducted in the PubMed/MEDLINE/Google Scholar databases. The research was supplemented with a manual search to identify related published articles in dental journals. Results Articles published from 1986 onwards, in English language with full texts, were selected. Our search revealed that only 6 cases of children with pleomorphic adenoma in cheek (including pleomorphic adenoma arising from accessory parotid gland) were reported in the last 34 years. This paper also reports a case of pleomorphic adenoma on the cheek in a 10-year-old girl. The mass diagnosed with Ultrasonography was removed by wide local excision and no recurrence has been noted with a follow-up period of 14 months. Conclusion Salivary glands tumors are very rare in the pediatric age group, and rarely in minor salivary glands. Proper examination and assessment with preoperative imaging are necessary for its diagnosis. Pleomorphic adenoma should be included in the differential diagnosis of cheek lesions in young and adult patients. The possibility of late recurrences and malignant transformation should be regarded, thus prolonged follow-up is essential.

Cysteine-Rich Secretory Proteins (CRISPs) From Venomous Snakes: An Overview of the Functional Diversity in A Large and Underappreciated Superfamily.

The CAP protein superfamily (Cysteine-rich secretory proteins (CRISPs), Antigen 5 (Ag5), and Pathogenesis-related 1 (PR-1) proteins) is widely distributed, but for toxinologists, snake venom CRISPs are the most familiar members. Although CRISPs are found in the majority of venoms, very few of these proteins have been functionally characterized, but those that have been exhibit diverse activities. Snake venom CRISPs (svCRISPs) inhibit ion channels and the growth of new blood vessels (angiogenesis). They also increase vascular permeability and promote inflammatory responses (leukocyte and neutrophil infiltration). Interestingly, CRISPs in lamprey buccal gland secretions also manifest some of these activities, suggesting an evolutionarily conserved function. As we strive to better understand the functions that CRISPs serve in venoms, it is worth considering the broad range of CRISP physiological activities throughout the animal kingdom. In this review, we summarize those activities, known crystal structures and sequence alignments, and we discuss predicted functional sites. CRISPs may not be lethal or major components of venoms, but given their almost ubiquitous occurrence in venoms and the accelerated evolution of svCRISP genes, these venom proteins are likely to have functions worth investigating.

A Novel Mutant of rLj-RGD3 (rLj-112) Suppressed the Proliferation and Metastasis of B16 Cells through the EGFR Signaling Pathway.

Lj-RGD3, which contains three Arg–Gly–Asp (RGD) motifs, was first identified from the buccal glands of Lampetra japonica and has been shown to suppress the tumor progression in the previous studies. Apart from the three RGD motifs, Lj-RGD3 is also characterized by its high content of histidine in its amino acid sequence. In order to clarify whether the histidine-rich characterization of Lj-RGD3 is also associated with its anti-tumor activity, mutants were designed in which the three RGD motifs (Lj-112), or all histidines (Lj-27) or both (Lj-26) were deleted. Furthermore, a mutant (Lj-42) in which all histidines and three RGD motifs were respectively substituted with alanines and three Ala–Gly–Asp (AGD) motifs, as well as a mutant (Lj-41) in which all histidines were substituted with alanines was synthesized to avoid alterations in structure which might further cause changes in the peptides’ functions. After recombination and purification, recombinant Lj-112 (rLj-112), recombinant Lj-27 (rLj-27), recombinant Lj-41 (rLj-41), and recombinant Lj-RGD3 (rLj-RGD3) exhibited anti-proliferative activity in B16 cells, respectively; while recombinant Lj-26 (rLj-26) and recombinant Lj-42 (rLj-42) did not affect the proliferation of B16 cells significantly. In addition, the anti-proliferative activity of rLj-112 in B16 cells was due to apoptosis. Typical apoptosis features were observed, including chromatin condensation, fragmented DNA, and increased levels of cleaved caspase 3/caspase 7/nuclear enzyme poly (ADP-ribose) polymerase (PARP) in B16 cells. Similar to rLj-RGD3, rLj-112 was also capable of suppressing the migration and invasion of B16 cells by disturbing the F-actin arrangement. After labeling with FITC, rLj-112 was found localized in the cytoplasm of B16 cells, which induced the internalization of epidermal growth factor receptor (EGFR), suggesting that rLj-112 might block the EGFR mediated signaling pathway. Actually, the phosphorylation level of EGFR and its downstream signal molecules including Akt, PI3K, p38, and ERK1/2 was reduced in the rLj-112 treated B16 cells. In vivo, rLj-112 also inhibited the growth, weight, and volume of the tumors in B16 xenografted C57BL/6 mice without reducing their body weight, indicating that rLj-112 might be safe and might be used as an effective anti-tumor drug in the near future.

The Anti-Angiogenic Activity of a Cystatin F Homologue from the Buccal Glands of Lampetra morii.

Cystatins are a family of cysteine protease inhibitors which are associated with a variety of physiological and pathological processes in vivo. In the present study, the cDNA sequence of a cystatin F homologue called Lm-cystatin F was cloned from the buccal glands of Lampetra morii. Although Lm-cystatin F shares a lower homology with cystatin superfamily members, it is also composed of a signal peptide and three highly conserved motifs, including the G in the N-terminal, QXVXG, as well as the PW in the C-terminal of the sequence. After sequence optimization and recombination, the recombinant protein was expressed as a soluble protein in Escherichia coli with a molecular weight of 19.85 kDa. Through affinity chromatography and mass spectrometry analysis, the purified protein was identified as a recombinant Lm-cystatin F (rLm-cystatin F). Additionally, rLm-cystatin F could inhibit the activity of papain. Based on MTT assay, rLm-cystatin F inhibited the proliferation of human umbilical vein endothelial cells (HUVECs) dose dependently with an IC50 of 5 μM. In vitro studies show that rLm-cystatin F suppressed the adhesion, migration, invasion, and tube formation of HUVECs, suggesting that rLm-cystatin F possesses anti-angiogenic activity, which provides information on the feeding mechanisms of Lampetra morii and insights into the application of rLm-cystatin F as a potential drug in the future.

Anatomic and magnetic resonance imaging features of the salivary glands in the dog.

The aim of the study was to describe the anatomical location and the topographic relationship of the major salivary glands to associated tissues in six dog cadavers by high-field magnetic resonance imaging (MRI) and gross anatomical dissections and sections. Spin-echo T1-weighted and T2-weighted magnetic resonance (MR) images were obtained in transverse, sagittal, and dorsal planes with a 1.5 T magnet. The digastric muscle, the external auditory canal, the eyeball, and the medial pterygoid muscle were the most consistent topographic landmarks for the identification of the mandibular, parotid, and zygomatic salivary glands, respectively. The best differentiation and contrast of the glands was observed in the T2-weighted images. In contrast with the osseous and muscular tissues, the salivary glands appeared hyperintense in greater or lesser measure except for the parotid gland, which displayed a hypointense signal. The rostral part of the monostomatic sublingual gland was precisely located between the tongue and the mylohyoid muscle. The best delineation between the caudal part of the monostomatic sublingual gland and the cranial aspect of the mandibular gland was observed in dorsal MRI at the level of the palate and the branches of the superior dental arcade. Good evaluation of the mandibular, parotid, caudal part of the monostomatic sublingual, zygomatic and buccal ventral glands was possible in sagittal MRI in close relation with the external auditory canal and the temporomandibular joint. The ventral buccal glands were also observed near the buccal vestibule as was the palatine glands within the soft palate.

Proteomic analysis of buccal gland secretion from fasting and feeding lampreys (Lampetra morii)

BackgroundPrevious studies have shown that lamprey buccal glands contain some regulators related to anticoagulation, nociception, and immune responses due to the blood sucking habit. Regrettably, the protein expression profile in the buccal glands of feeding lampreys has never been reported yet. The present study was performed in order to further identify more proteins which are closely associated with lamprey feeding process.Methods2D-PAGE, NanoLC–MS/MS with higher resolution, Ensembl lamprey and NCBI protein databases, as well as western blot was used to compare the proteomics of buccal gland secretion from China northeast lampreys (Lampetra morii) which had been fed for 0, 10, and 60 min, respectively.ResultsIn the present study, the number of identified protein species in the buccal glands of feeding groups (60 min) was increased significantly, nearly ten times of that in the fasting group. During the feeding stage, novel proteins emerged in the buccal gland secretion of lampreys. According to gene ontology (GO) analysis and function predictions, these proteins were summarized and discussed based on their potential roles during feeding process. Furthermore, some of the identified proteins were confirmed to express during the feeding time of lampreys.ConclusionWhen lampreys attack host fishes to suck blood and flesh, their buccal glands could secrete enough proteins to suppress blood coagulation, nociception, oxidative stress, immune response, as well as other adverse effects encountered during their parasitic lives. The present study would provide clues to clarify the feeding mechanism of the bloodsucking lampreys.

An improbable opportunistic predator: the functional morphology ofPinna nobilis(Bivalvia: Pterioida: Pinnidae)

The functional morphology ofPinna nobilisis described, with special reference to the uniquely pinnid pallial organ, the similarly unique buccal (formerly pallial) gland, the stomach and its contents. The pallial gland produces sulphuric acid which as well as functioning as a shell cleaning swab may be involved in prey capture. The buccal gland discharging into the oesophagus has proteolytic digestive functions while the stomach is adapted for the reception and digestion of captured, mucous-bound, mesozooplanktonic and epi- and endo-benthic, prey items.Pinna nobilisis thus not simply either an accidental or incidental predator of such species but is opportunistic. The buccal glands and stomachs of other Pinnidae are not so specialized as inP. nobilis,possibly indicating that in the particular, oligotrophic, environment of the Mediterranean and Adriatic Seas, and in which it is endemic,P. nobilishas, in addition to being a typical ctenidial suspension feeder, become an opportunistic predator. Although the deep sea representatives of the Septibranchia (Anomalodesmata) and Propeamussidae are obligate predators, this is the first record of any bivalve functioning as an opportunistic predator with unique morphological adaptations to facilitate this.

Explorative study on mucosal and major salivary secretion rates, caries and plaque microflora in head and neck cancer patients.

To analyse mucosal and major salivary secretion rates, caries and plaque microflora in connection with treatment for cancer in the head and neck region. Thirty-three patients were included and the number of teeth, filled surfaces and caries lesions registered. The labial and buccal gland secretion and stimulated whole salivary secretion rates were determined. Supragingival plaque microflora was analysed using cultivation technique. Data were collected pretreatment, during treatment and 6months, 1year and 2years post-treatment. Two years post-treatment, 36% had new caries lesions, which had been restored. The labial secretion was comparable with pretreatment, while the buccal secretion was lower (P<.001). The stimulated secretion rate was lower compared with pretreatment (P<.001) and was ≤0.7mL/minute for 50%. Growth of lactobacilli increased during treatment (P<.001) and remained increased (P<.001), while growth of mutans streptococci was decreased (P<.01) 2years post-treatment. Growth of Candida increased over time and was higher 2years post-treatment compared with pretreatment (P<.001) while growth of Prevotella was lower (P<.01). Two years post-treatment, the stimulated salivary secretion rate was substantially decreased, acid-tolerant lactobacilli and Candida increased, acid-sensitive microorganisms decreased, and the caries prevalence was low.

Pleomorphic Adenoma of Buccal Minor Salivary Gland: A Case Report

Salivary gland tumors are relatively uncommon tumors, accounting approximately 3-10% of head and neck neoplasm. Most of them are benign and pleomorphic adenoma is the commonest among them that involves both major (90%) and minor (10%) salivary glands. The most predominant site of pleomorphic adenoma is palate; however, it may occur in any part of oral cavity like lip, floor of mouth, tongue, tonsil, pharynx, retromolar area and nasal cavity. Mucosa of cheek (4%) is an extremely rare site. A case of 25 years young male is reported here possessing pleomorphic adenoma of buccal minor salivary gland that was presented to us with a mobile swelling over right cheek that found to be pleomorphic adenoma of buccal minor salivary gland after histopathological examination. Surgical excision was done by intra oral approach with adequate margins. Patient had an uneventful postoperative course and discharged same day on oral antibiotics.

The PR-1 domain accounts for the anti-angiogenic activity of a cysteine-rich secretory protein member from the buccal glands of Lampetra japonica

Previous studies have shown that cysteine-rich buccal gland protein (CRBGP) from buccal glands of Lampetra japonica could suppress angiogenesis in chick chorioallantoic membrane models. As CRBGP is composed of a pathogenesis-related group 1 (PR-1) domain and a cysteine-rich domain (CRD), which domain accounts for the effects of CRBGP on anti-angiogenesis? In the present study, recombinant PR-1 and CRD (rL-PR-1 and rL-CRD) were obtained. MTT assays showed rL-PR-1 inhibited the proliferation of HUVECs significantly in a dose-dependent manner with an IC50 of 2μM, while rL-CRD had no obviously inhibitory effect on the proliferation of HUVECs, suggested that PR-1 is the main function domain on the anti-angiogenic activity of CRBGP. Similar to CRBGP, rL-PR-1 induced apoptosis in HUVECs in a mitochondrial-dependent pathway by affecting the level of BAX, BCL2 and caspase 3. Also, the cytotoxic property of rL-PR-1 might be one of the factors which suppressed the proliferation of HUVECs. Furthermore, rL-PR-1 blocked the adhesion, migration, invasion and tube formation of HUVECs by disturbing the cytoskeleton arrangement and down-regulating the level of matrix metallo-peptidase 2. In summary, rL-PR-1 has the anti-angiogenic activity which would provide the information on the functions and mechanisms of cysteine-rich secretory protein family members.