Broiler Parts Research Articles

Concentrations, Sources, and Health Risk of Heavy Metals in Edible Parts of Broilers from Northeast of Algeria.

Heavy metals contamination of poultry products is a major concern for public health. This study aimed to determine the concentration of mercury (Hg), lead (Pb), and iron (Fe) in the edible parts of broilers, as well as in feed, drinking water, and litter as sources of contamination and to assess their possible human health risk in the province of Jijel (Northeast Algeria). The range of Hg, Pb, and Fe in edible parts were 0.004-0.007, 0.185-0.480, and 28.536-88.306mg/kg, respectively, and the difference in content was only significant (p < 0.05) for lead. Breast and thigh samples had Pb concentrations above the maximum limit. Spearman coefficient analysis revealed that most correlations were positive between metals detected in feed, water, and litter and those in the edible parts of broilers. But most of them were insignificant (p > 0.05). The estimated daily intake (EDI) values of examined metal exceeded the tolerable daily intake (PTDI). The target hazard quotients (THQ) and hazard index (HI) of all metals were lower than 1, suggesting no significant carcinogenic risks. The calculated incremental lifetime cancer risk (ILCR) of Pb was higher than 10-4 for men, women, and children, indicating the presence of carcinogenic risk. Considering the wide consumption of broiler meat, regular national monitoring of heavy metals in the broiler production chain is recommended to protect population health.

Effects of Protease and Sex on Skin, Bone and Muscle Yields and Percentages of Major Primal Cuts in Broilers

Summary This study aimed to assess the effects of different dietary levels of the protease enzyme and sex on skin, bone and muscle yields and percentages of breast, drumsticks and thighs as the most valuable carcass parts of broilers. A total of 300 one-day-old, mixed-sex broiler chickens (Cobb 500) were randomly divided into 3 dietary treatments with different levels of protease supplementation (group C: a standard diet without Ronozyme ProAct; group E-I: a diet with 200 mg/kg Ronozyme ProAct and a 4% crude protein reduction compared to the standard diet; and group E-II: a diet with 300mg/kg Ronozyme ProAct and a 6% crude protein reduction compared to the standard diet). All diets were maize- and soybean meal-based. Feed and water were provided ad libitum throughout the 49-day feeding trial. The results obtained indicate that the dietary treatments had no significant effect on skin, bone and muscle yields and percentages of the drumsticks, thighs and breast (P &gt; 0.05), except for the breast muscle weight in the female chickens (especially between the E-I and E-II groups), and the drumstick bone percentage in the males between the C and E-2 groups (P &lt; 0.05). In all three groups, the male chickens exhibited higher weights of skin, muscle and bone in breast, drumsticks and thighs than the female chickens, and the differences were not significant only in the skin weight of drumsticks and thighs (P &gt; 0.05). The females had a higher yield of muscle tissue in breast and drumsticks, a higher percentage of skin in drumsticks and thighs, and a lower percentage of bone in drumsticks and thighs than the males (P &lt; 0.05). Major differences resulted from the effect of broiler sex, whereas the dietary treatments containing 3 levels of protease supplementation had a very small effect on the yields and percentages of skin, bone and muscle in breast, drumsticks and thighs.

Effects of organic zinc on production performance, meat quality, apparent nutrient digestibility and gut microbiota of broilers fed low-protein diets

The high cost of feed and nitrogen pollution caused by high-protein diets have become major challenges restricting sustainable development in China's animal husbandry sector. Properly reducing protein levels and improving protein utilization in feed are effective approaches to solving this problem. To determine the optimal dose of methionine hydroxyl analogue chelated zinc (MHA-Zn) in broiler diets with a 1.5% reduction in crude protein (CP), a total of 216 1-day-old broilers were randomly assigned into 4 groups (each group consisted of 3 replications with 18 broilers per replicate), and growth and development indexes were assessed after 42 days. The broilers in control group were fed a basic diet, whereas those in the three test groups were fed diets with a 1.5% reduction in CP. The results showed no significant difference in the edible parts of broilers between low-protein (LP) diet group (90 mg/kg MHA-Zn) and normal diet group (p > 0.05), and adding 90 mg/kg MHA-Zn to LP diet significantly improved ileum morphology and apparent total tract digestibility (ATTD) of nutrient (p < 0.01; p < 0.05). A 16S rRNA sequencing analysis indicated that supplementing the LP diet with 90 mg/kg MHA-Zn was adequate for production performance of broilers and promoted beneficial bacteria in the cecum (Lactobacillus, Butyricoccus, Oscillospira, etc.) (p < 0.01). In summary, adding an optimal dose of organic zinc (90 mg/kg MHA-Zn) in low protein diets led to enhanced production performance of broilers and optimized cecum microbiota. Additionally, the reduction of crude protein consumption in broiler production proved to be a cost-effective measure, while also mitigated nitrogen pollutant emissions in the environment.

Does environmental enrichment improve performance, morphometry, yield and weight of broiler parts at different ages?

ABSTRACT The inclusion of environmental enrichment in conventional broiler rearing can increase mobility, bone mass and muscle. This research aimed to evaluate the use of environmental enrichment in the rearing of broilers at different ages and its influence on performance, morphometry, yield and weight of the parts. It was used the completely randomized design in split-plot scheme, being the plots the treatments presence (T1) and absence (T2) of environmental enrichment and the subplots the broiler ages (1, 7, 14, 21, 28, 35 and 42 days), with 56 chicks in each treatment (T1 and T2). Data obtained were analyzed by the linear effect model of fixed effects and compared by Tukey’s test of means. The animals were raised in a controlled environment, divided into four boxes with dimensions: 1.5 × 1.0 × 0.7 m, containing rice straw bed. In T1 a ladder with a perch on top was used, distributed every 1.5 m2. Environmental enrichment used did not influence broiler’s zootechnical performance. Broilers’ morphometric properties, parts weight and body weight increased due to environmental enrichment and, improvement was observed in chicks rearing’s final phase. The environmental enrichment was beneficial for muscle and bone mass gain in the main commercial parts of the chicken carcass, in addition to reducing the allometric coefficient of the breasts in chickens.

Pengaruh Ransum Basal Dan Feed Additive yang Berbeda Terhadap Bobot, Persentase Karkas Dan Recahan Karkas Ayam Broiler

The objective of the present study was to evaluate the effect of different basal diet and feed additive on the percentage of carcass and component parts of broilers. This research has been conducted for four months (June to September 2020) at State Polytechnic of Agriculture Kupang. A total of 264 one-day old chicks were distributed to 24 pens (11 birds/pen).The experiment was designed using a 2 x 4 factorial design with 8 tretment combinations and three replications for each treatment. The treatments were control (0% sago) (P0), control (0% sago) + avyzime 0,10 % and phyzyme 0,05% (P1), control (0% sago) + allzime 0,05% (P2), control (0% sago) + synbiotic probio FMplus 40 mL/L drinking water (P3), Diet containing 10% sago (P4), Diet containing 10% sago + allzyme 0,10 % and phyzyme 0,05% (P5), Diet containing 10% sago + allzyme 0,05% (P6) and Diet containing 10% sago + synbiotic probio FMplus 40 mL/L drinking water (P7). The result shows that type of basal diet (TBD) and feed additive (FA) affect (P &lt; 0,05) the carcass weight but it did not afect (P&gt;0,05) carcass percentage of broiler chickens. Except for thigh, TBD did not affect (P&gt;0,05) on component parts of carcass. Feed additive had no effect (P&gt;0,05) on carcass component, with the exception of thigh. Interaction between TBD x FA was significant (P&lt;0,05) only for weight and percentage of carcass and back percentage of broilers. Broilers fed basal diet A plus Allzyme SSF-E had lower weight and percentage of carcass and also back percentage than other treatment diets. To sum up,, 1) basal diet B produced higher carcass weight than basal diet A; 2) supplementation of Allzyme SSF-E on basal diet A reduced the weight and percentage of broiler carcass.

Predicting the extent to which excess lipid is deposited in the physical components of a broiler when dietary protein content is reduced

Context The weight of each of the physical parts of the body of broilers, predicted using their allometric relationship with feather-free body protein, differs with the level of dietary protein offered. Aims The objective of this study was to account for the excess lipid that is deposited differentially in the physical parts of the body of broilers when dietary protein content is decreased. Methods In total, 2496 day-old Cobb 500® and Ross 308® broilers, equally divided between males and females, were used in a 56-day feeding trial. The experimental design used was a response experiment with six balanced protein concentrations (0.60, 0.70, 0.85, 1.00, 1.15 and 1.30 of the recommendation), with two factors (males and females) and two strains (Cobb and Ross). On Days 14, 28, 42 and 56 post-hatch, eight broilers from each feed × sex × strain combination were euthanised and partitioned into breast, legs, wings, and remainder. Each component was weighed and subsequentially analysed for water, protein and lipid. Allometric equations between the component weights and body protein weight were fitted to describe the responses. Key results In the allometric equations used to describe the additional weight of each component, at a given body protein weight, resulting from the additional amount of lipid that is deposited in the component as a result of reducing the dietary protein content, only the constant terms were affected. By expressing these constant terms as a proportional increase above the genetically determined level of fatness, described by males on the highest dietary protein feed, equations were derived that described the rate of increase in lipid weight with a change in dietary protein content. Conclusions When predicting the weights of different components in the body by using the allometric relationships between the component weight and body protein weight, equations are now available to correct the weights of the respective components of broilers for the additional amount of lipid that would be deposited as a result of feeding dietary protein contents below that required to achieve the genetically desired level of fatness in each component. Implications With the equations presented herein, one can predict the weights of commercial broiler parts, considering the extra fat deposited due to the dietary balanced protein offered, which may lead to decisions that increase the economic return of poultry production.

Profitabilitas dan Efisiensi Usaha Ternak Ayam Broiler di Tasikmalaya Jawa Barat

The key of the Indonesian government as far as the livestock sector is concerned is the expansion of the poultry industry, in particular the broiler industry. Policy maskers see the broiler industry as an important source of animal protein since in comparison with cattle poultry are efficient converters of feed grains into meat. The research area is Tasikmalaya, as is considered to be a potential broiler production area that develops better among regions in the West Java. This study is focused on broiler business pattern, both partnership and autonomous, using the profitability and efficiency to measure the tingkat of comparative advantage. Policy Analysis Matrix (PAM) based on primary data were constructed for the different pattern of production system. Scale of production was also taken into account. The larger were farmers who exerted more than 2000 heads per production cycle. The study find that broiler production in Tasikmalaya is an efficient industry and can exist without any protection. The partnership and the larger farmers enjoy more profit relative to the autonomous and the smaller ones. Tingkats of efficiency and profitability are influenced by production factor prices of feed and DOC as well as broiler. Other finding is that government plant to set 80% imported tariff on chicken leg quarter (broiler part) can not avoid yet to makes the domestic product of broiler less competitive in the domestic market. Key Words: efficiency, profitability, competitive advantage.

Assessment of Stabilized Hydrogen Peroxide for Use in Reducing Campylobacter Levels and Prevalence on Broiler Chicken Wings

Poultry processing establishments use antimicrobial aids on broiler parts to minimize Campylobacter contamination. A silver-stabilized hydrogen peroxide (SHP) product was assessed for use as an antimicrobial processing aid. In a series of experiments, wing segments with skin were inoculated with 103 to 107 cells of Campylobacter coli, followed by treatment with SHP at 15,000 or 30,000 mg/L, peroxyacetic acid (PAA) at 300 or 3,000 mg/L (parts per million), or water. Each treatment was applied by either dip or spray. Rinsates from each wing segment were analyzed for direct counts and prevalence of Campylobacter. Treatment with SHP or PAA significantly reduced Campylobacter levels compared with water controls by up to 2.22 log CFU/mL. At high inoculum levels (106 to 107), SHP and PAA applied by dip had up to 1.27 log CFU/mL further reductions of Campylobacter levels compared with spray-treated wing segments. Additionally, wing drumettes were observed to retain higher levels and prevalence of Campylobacter recovery compared with wing flats at a low inoculation level (103). The results indicated that there was no carryover effect of SHP (same day versus 24 h) and dip treatment with SHP or PAA decreased Campylobacter recovery on broiler chicken wing segments compared with a water control. Although a 2-log reduction was modest, SHP had similar efficacy as the commonly used processing aid PAA. SHP shows potential for further investigation as an antimicrobial processing aid for use on poultry parts.

Impact of both early-age acclimation and linseed dietary inclusion on fat deposition and fatty acids' meat traits in heat-stressed broiler chickens.

Objective:The purpose of this work was to investigate the combination of early-age acclimation and linseed dietary inclusion in enriching polyunsaturated fatty acids (PUFAs) in broilers’ meat as a strategy to mitigate heat stress.Materials and Methods:A total of 400 broiler chicks were assigned to four experimental groups with four duplicates (25 animals each): C: control (basal diet), AC: early-age acclimated (basal diet), Cl: fed 5% ground linseed, and Acl: early-age acclimated and fed 5% ground linseed. The lipid and fatty acid contents of different parts (breast, thigh, liver, subcutaneous, and abdominal fat) of broilers were determined.Results:Low levels of lipids and unsaturated fatty acids have been found in the meat of acclimated broilers. Higher levels of linolenic acids were noted in Cl thigh meat compared to C (6% vs. 2.68%, respectively). The results showed that oleic and linoleic acids constitute a large part of the PUFAs of different meats. The most elevated levels of monounsaturated fatty acids were recorded in the breast meat of AcL animals. The highest content of omega-3 was recorded in the liver of AcL animals compared to that of C (14.98% vs. 7.8%, respectively). Conclusion:We suggest that the combination of treatments during hot conditions has led to the reversion of the environment-affected variables to accepted values, and yields better thermoresistance, PUFA-enriched meat, and safeguard animal health which conferred to birds’ better solutions to reduce fatigue and hypoxic activities, which induces a considerable consumption of oxygen.

REDUCTION OF CAMPYLOBACTER ON POULTRY THIGHS USING SEQUENTIAL TREATMENTS OF ANTIMICROBIALS

Campylobacter is a major concern for poultry processors, as USDA performance standards have become stricter. This study evaluated CMS PoultrypHresh™, a low pH processing aid, and peracetic acid using consecutive and sequential dip treatments to reduce Campylobacter in thighs. Thighs (n=3/treatment group) were inoculated with a C. coli marker strain (108) and each dipped into bags containing 1 L of treatment 1 for 6 s. Thighs were allowed 5 s to drip, placed onto foil for 60 s, and dipped into treatment 2 for 6 s. After 5 s drip time, each was placed in a bag with 150 mL buffered peptone water and hand shaken for 60 s; controls same procedure, no treatment. Rinsates were serially diluted, plated onto Campy Cefex agar with 200 ppm gentamicin and incubated microaerobically for 48 h at 42°C. Procedures were replicated 5 times. Significant reductions compared to untreated using consecutive dips of PoultrypHresh™ and PAA were 98.2% and 99.3%, respectively. Treatments of PoultrypHresh™ then peracetic acid reduced Campylobacter 99.2% from untreated thighs. Peracetic acid then PoultrypHresh™ showed significant reductions compared to all other treatments (99.9% from untreated). Treating with this sequence may allow processors to meet the strict performance standards on Campylobacter in broiler parts.

PENAMPILAN KARKAS AYAM PEDAGING DENGAN PEMBERIAN KULIT KOPI (Coffea sp) PENGOLAHAN SEDERHANA SUBSTITUSI SEBAGIAN JAGUNG DENGAN LEVEL YANG BERBEDA

CARCASS PERFORMANCE OF BROILER FED RATION CONTAINING COFFEE (Coffea sp) HUSK WASTE PRODUCT TREATED BY SIMPLE PROCESSING AND SUBSTITUTING PARTS OF CORN WITH DIFFERENT LEVELS. Broilers had rapid growth rate and need high nutrient protein and energy balance in the diet. This study was conducted to evaluate the use of coffee husk waste product treated by simple processing substituted with part of corn in ration on carcass percentage of broiler. Study was applied at Faculty of Animal Science, Sam Ratulangi University during 35 days. Total of 144 day old chick unsexed broilers, strain of CP 707, involving 36 experimental units of battery pens were used in this study. Each unit of pen was filled 4 birds under completely randomized design on factorial patter of 3x4. Factor A was focused on three processing treatments including coffee husk heated under sun light (A1), coffee husk immersed in the water (A2), and coffee husk boiled under water (A3). Factor B was focused on four treatments of substitution levels including ration containing corn without coffee husk substitution (B0), ration containing corn substituted with 25% coffee husk (B1), ration containing corn substituted with 50% coffee husk (B2), ration containing corn substituted with 75% coffee husk (B3). Variables observed were including percentages of thigh, wing, breast and back part. Analysis of variance showed that interaction of processing treatments did not affect significantly on the percentages of thigh, wing, breast and back part. This study indicated that simple processing of coffee husk were not affected the carcass percentages of broiler. The substitution levels significantly affected carcass percentage of broiler. Percentages of thigh, wing, breast and back part were 28.92%-30.98%, 10.38%-12.68%, 35.10%-38.55% and 19,68%-22,71%, respectively. This study showed that utilization of coffee husk waste product by above simple processing could substitute part of corn in ration of 25% producing high carcass percentage of broiler.Keywords: Coffee husk by product, broiler part of carcass percentage

Application of antimicrobial treatment to whole carcasses during prechill can improve microbial quality of broiler parts

AbstractBroilers cut up into parts have been reported to have higher microbial numbers than corresponding whole carcasses. Prechill immersion leads to water uptake by whole carcasses which is released during cut‐up. The objective of this study was to determine if an antimicrobial treatment applied during prechill can reduce the prevalence or numbers of total aerobic bacteria and Escherichia coli on corresponding chicken parts. Commercially eviscerated broiler carcasses were subjected to sequential immersion prechill, final ice water chill, and cut‐up procedures. Enumeration of total aerobic bacteria, coliforms, and E. coli was performed using carcass rinses at postevisceration, after 15 min prechill (22–25°C water), after 30 min ice water immersion chill (0–4°C water), and following cut‐up into parts. Six prechill, antimicrobial treatments were evaluated: 50 ppm chlorine, 50 ppm chlorine + 0.5% T‐128 (a chlorine stabilizer), 20 ppm peracetic acid, 20 ppm peracetic acid + 0.5% T‐128, 0.5% T‐128, and water. Addition of 0.5% T‐128 to water, 20 ppm peracetic acid, or 50 ppm chlorine during prechill significantly (p &lt; .05) decreased total aerobic bacteria and E. coli/coliforms following prechill by ∼1.5–2.0 log compared to the water control.Practical applicationsAntimicrobial treatment of whole broiler carcasses during prechill is a practical means to lessen microbial contamination of corresponding broiler parts. Chlorine and peracetic acid applied with a chlorine stabilizer (T‐128) can be expected to be effective for this purpose.

Efficacy of Bacillus subtilis and bacitracin methylene disalicylate on growth performance, digestibility, blood metabolites, immunity, and intestinal microbiota after intramuscular inoculation with Escherichia coli in broilers

This experiment was conducted to evaluate the effect of Bacillus subtilis (BS) on broiler performance and health after intramuscular inoculation with E. coli and compare its effect with a growth promoter antibiotic. In a completely randomized design manner, 360 male Ross 308 chicks were divided into 6 treatments and 5 replicates of 12 chicks in each replicate. Experimental treatments included control diet, control + E. coli (0.5 mL of culture containing 108 CFU of E. coli/ml), control + 0.1% BS, control + 0.05% bacitracin methylene disalicylate (BMD), control + E. coli and BS, and control + E. coli and BMD in a factorial arrangement (3 × 2). Addition of BMD or BS to the control diet significantly (P < 0.01) increased body weight and decreased FCR, but E. coli challenge adversely reduced (P < 0.01) body weight and increased FCR, so that the addition of BMD or BS did not compensate growth reduction. E. coli challenged chicks had the lowest vaccine titers for ND, IB, AI, and IBD and the highest were observed in chicks fed BS. The E. coli challenge significantly (P < 0.01) increased albumin, globulin, cholesterol, triglyceride, LDL, ALT, and ALP indices. Addition of BMD and BS decreased albumin and globulin in challenged chick's plasma but had no effect on plasma lipid profile concentration. The E. coli challenge decreased villus height and increased crypt depth and goblet cell numbers significantly (P < 0.01). In birds subjected to BMD or BS, crypt depth decreased and villus height increased (P < 0.01), compared with the control diet. Challenge of E. coli significantly (P < 0.01) increased the bacterial population of E. coli, coliforms, and Salmonella in cecal parts of broilers’ intestines. In challenged birds receiving BMD or BS, E. coli, coliform, and Salmonella populations of ceca showed a significant (P < 0.01) reduction. Both BMD and BS increased the digestibility of nutrients significantly (P < 0.01), but a reduction was observed in E. coli challenged groups. Results of the study suggest that spore-forming probiotics are partially effective in unsuitable rearing situations such as colibacillosis in which the load of harmful bacteria is high.

Treatment with a low pH processing aid to reduce Campylobacter counts on broiler parts

New regulations and performance standards for Campylobacter have been implemented by the USDA - Food Safety and Inspection Service (FSIS). The objective of this study was to evaluate treatment with a low pH processing aid (CMS1 PoultrypHresh), a formulated low pH processing aid, to reduce numbers of Campylobacter which could help companies meet regulatory requirements. Two experiments (3 replicates each) were conducted. Experiment 1, in each of 3 replicates, skin-on split chicken breasts (n = 15) were obtained from a local grocery and divided into groups of 5. The skin of each part was inoculated with approximately 107 cells of a gentamicin resistant C. coli (CCGR) marker strain in an area of approximately 6.5 cm2. CCGR cells were allowed to attach for 5 min prior to treatment. Ten inoculated breasts were individually placed into separate 6 L plastic storage boxes containing either 3.5 L deionized water or PoultrypHresh solution at a pH of 1.4. Parts were subjected to agitation (bubbled air) for 25 s. After treatment, each part was removed, allowed to drain for 5 s, and placed into a plastic bag prior to mechanical rinsing with 150 mL of buffered peptone water for 60 s. Five inoculated breasts served as controls, were untreated with a dip or agitation and sampled as above. Experiment 2 procedures were repeated using skin-on thighs under the same conditions. Rinsates were collected from each chicken part, serially diluted, and plated onto Campy Cefex agar with 200 ppm gentamicin (CCGen). All plates were incubated microaerobically (5% O2, 10% CO2, 85% N2) for 48 h at 42°C, colonies were counted and the cfu/mL was log transformed. The use of PoultrypHresh on split breast produced a 99.6% reduction compared to untreated controls, while thighs showed a 99.4% reduction. This study demonstrated an approximate 3 log reduction (P < 0.05) using a 25 s air agitation treatment in PoultrypHresh at pH 1.4 with no observable damage, which will help processors meet FSIS regulations.

Effect of Different Dress Weight Categories on Yield Part Percentage and Relationship of Live and Dress Weight of Broiler Carcasses Slaughter at Different Conditions

Commercially reared mixed sex broilers were utilized to determine the effect of dress carcass weight on percentage yield of broiler parts, breast meat yield in weight category of &lt;1.0, 1.0-1.2, 1.2-1.4, 1.4-1.6, 1.6-1.8, 1.8-2.0 and &gt;2.0 kg. Then pH, cooking yield and moisture content of the breast meat with different time intervals (0 hours, 12 hours, 24 hours, 36 hours, 48 hours) and proximate compositions of meat were measured. Percentage yield of whole leg, thigh, breast, back and rib, neck, wings, gizzards, heart, liver, drumstick and total giblet were significantly affected by dress carcass weight at p&lt;0.05. Yield of body components changed with the increasing body weight. pH values was significantly affected by time after slaughtering and finally it's reduced up to 5.69 at 48 hr after slaughtering. Suitable processing weight of broiler carcass to gain maximum breast yield is &gt;2.0 kg which could be obtained highest fillet yield. To gain highest cooking yield (83.55%), carcass should be cooked immediately after slaughtering. Regression analyses revealed that linear relationship between live weight and dress weight of broiler carcass at different transport distance, starvation period, life time, and cage system at p&lt;0.01 except &gt;150 kg transport distance. Based on the dressing percentage, weight of total edible meat, it may be concluded that broiler chickens are optimally slaughtered up to 2.0 kg live weight. Live and dress weight had highly significant relationship with the transport distance, starvation period, life time, cage system and live weight category.

Utilization of buffered vinegar to increase the shelf life of chicken retail cuts packaged in carbon dioxide

Poultry processors commonly place whole parts of broilers in plastic packages and seal them in an atmosphere of 100% carbon dioxide before shipping them to food service and retail customers. This practice extends the shelf life of retail cuts to approximately 12 d under refrigerated conditions. The objective of this study was to determine the antimicrobial efficacy of vinegar for growth inhibition of mesophilic and lactic acid bacterial counts and enhancement of shelf life in CO2-packaged refrigerated chicken thigh samples. Meat quality, sensory differences, and microbial enumeration were evaluated for chicken thighs that were sprayed with 0, 0.5, or 1.0% vinegar. No differences were observed (P > 0.05) among treatments (control vs. 0.5 and 1.0% vinegar-treated chicken thighs) with respect to pH and Commission Internationale d'Eclairage L*a*b*for both chicken skin and the meat tissue. The difference from the control test indicated that trained panelists were not able to detect a difference (P > 0.05) in flavor between the chicken thigh treatments. The mesophilic and Lactobacillus bacterial counts were enumerated after 0, 4, 8, 12, 16, and 20 d of storage. The mesophilic bacterial load for the 1.0% vinegar treatment was less than all other treatments after 8, 12, 16, and 20 d of storage, whereas the 0.5% vinegar treatment had lower bacterial counts at d 12 than both controls and had an approximate shelf life of 16 d. For lactic acid bacteria, the vinegar 1.0% treatment had lower counts than the control treatments at d 12 and 16. The results from the study indicate that a combination of 1.0% vinegar with CO2 packaging can extend the shelf life from 12 to 20 d for chicken retail cuts without negatively affecting the quality and sensory properties of the broiler meat.

Is Poultry Exudate a Missing Link in Spreading Campylobacter SP. In Poland?

Abstract The aim of the work was to find out what is the prevalence and burden of Campylobacter sp. in the poultry exudates, what are the predominant species, how diverse they are, and can poultry exudates be considered as the essential carriers of campylobacters posing a health risk of campylobacteriosis in Poland. Broilers’ and turkeys’ exudates, collected directly from the bulk containers at the day of delivery were subjected to pre-enrichment and direct plate counting according to ISO 10272 (1995) with presumptive C. jejuni strains subjected to confirmatory genetic identification by the nested PCR and RAPD typing. The results indicated that over 90% of the exudates tested carried Campylobacter sp., with their number being significantly higher in broilers’ (103 to105 cfu mL-1) compared to turkeys’ (102 to 104 cfu mL-1) (P≤0.05), and essentially higher (P≤0.05) for the broiler parts’ “skin on” exudates. C. jejuni was the only species present in the turkeys’ and predominant in broilers’ parts’ exudates. RAPD-typing of the isolates revealed 14 distinct RAPD types with the majority of C. jejuni isolates considered to be closely related.

Assessment of human exposure to 3rd generation cephalosporin resistant E. coli (CREC) through consumption of broiler meat in Belgium

Acquired resistance of Escherichia coli to 3rd generation cephalosporin antimicrobials is a relevant issue in intensive broiler farming. In Belgium, about 35% of the E. coli strains isolated from live broilers are resistant to 3rd generation cephalosporins while over 60% of the broilers are found to be carrier of these 3rd generation cephalosporin resistant E. coli (CREC) after selective isolation. A model aimed at estimating the exposure of the consumer to CREC by consumption of broiler meat was elaborated. This model consists of different modules that simulate the farm to fork chain starting from primary production, over slaughter, processing and distribution to storage, preparation and consumption of broiler meat. Input data were obtained from the Belgian Food Safety agencies' annual monitoring plan and results from dedicated research programs or surveys. The outcome of the model using the available baseline data estimates that the probability of exposure to 1000 colony forming units (cfu) of CREC or more during consumption of a meal containing chicken meat is ca. 1.5%, the majority of exposure being caused by cross contamination in the kitchen. The proportion of CREC (within the total number of E. coli) at primary production and the overall contamination of broiler carcasses or broiler parts with E. coli are dominant factors in the consumer exposure to CREC. The risk of this exposure for human health cannot be estimated at this stage given a lack of understanding of the factors influencing the transfer of cephalosporin antimicrobial resistance genes from these E. coli to the human intestinal bacteria and data on the further consequences of the presence of CREC on human health.

Medidas morfológicas em função do peso e da idade da ave, por meio de imagens

Neste estudo objetivou-se quantificar a distribuição da temperatura superficial do corpo da ave, determinar a relação de proporção da taxa de perda de calor (TPC) e de resfriamento (TR) em função do peso e da idade da ave, além de realizar avaliações morfológicas por meio de análise de imagens. Os pintos de um dia ROSS 508 foram acompanhados semanalmente, durante 42 dias de idade e distribuídos em dois grupos de 10 animais. Em período igual cada ave foi pesada, fotografada e coletada a temperatura de cada parte do corpo. Os dados da temperatura das partes do corpo da ave foram avaliados através do PROC MIXED do SAS. Em ambas as medidas da temperatura das partes da ave ocorreu variabilidade não apresentando, no entanto, um comportamento definido com a idade. A taxa de perda de calor (TPC) aumentou com a idade e com o peso da ave. Apesar disto, a taxa de resfriamento (TR) foi inversamente proporcional ao peso da ave. A utilização de imagens digitais para estimar a área, o peso e as medidas morfológicas em função da idade das aves, é viável e pode ser utilizada no cálculo de transferência de calor e de massa para dimensionar sistemas de climatização dos aviários e também para o desenvolvimento de equipamentos utilizando-se visão computacional.

Efficacy of the Precure™ Antimicrobial1 (pH = 1.5) as a Dip Treatment (3 vs. 30 seconds) for Controlling Microorganisms on Broiler Parts

Efficacy of the Precure™ Antimicrobial1 (pH = 1.5) as a Dip Treatment (3 vs. 30 seconds) for Controlling Microorganisms on Broiler Parts