Broad Changes Research Articles

Responding to platform firm power: differing national responses

ABSTRACT Aware of the power of platform firms and the risks of the platform economy, governments are reconsidering the dominant laissez faire approach in favour of strategies that regulate the power of platform firms. transnational dynamics play an important role in shaping emerging national regimes, but we argue that governments are also influenced by three factors that operate primarily at domestic level: the agency of platform firms, the impact of first-mover US platforms, and domestic politics. We illustrate our approach through an analysis of the trajectories and regulatory measures of four economies: the US, the EU, China, and India. As governance efforts expand to emerging technologies such as AI, our analysis presages broader changes to the structure of what was, with a few important exceptions, a relatively borderless global online economy.

Culture as Nature

Metaphors are widely employed in academia and beyond to render complex concepts more accessible. This paper investigates the use of metaphors drawn from nature to approach culture and culture contact in the ancient Mediterranean, focusing on examples evoking the three fundamental states of matter: solid, liquid and gas. Although the Mediterranean is celebrated as ‘the mother of all metaphors’, this has not attracted any systematic analysis, nor has it been problematized. States of matter metaphors are widely used in the study of Mediterranean antiquity and can be shown to be more than figures of speech or heuristics; they convey particular modes of conceptualizing culture which depend on a range of empirical and theoretical approaches developed across the humanities and social sciences, including some that are outdated and that fail to capture the complexity of culture. My critical examination of such metaphorical thinking covers a heterogenous body of material that has become interwoven through selective and messy exchanges of ideas across different disciplines and between disciplines and popular culture. In such exchanges, the pedigree and conceptual load of associated terms is often forgotten or neglected. My aim is to identify the main threads and investigate how shifts in the use of states of matter metaphors for Mediterranean culture and culture contact relate to broader shifts in approaches to the topic. The exercise reveals how states of matter metaphors are also applied to different phases of the historiography of the ancient Mediterranean, as well as to the periodization of modernity. Liquid metaphors are found to predominate, solid ones are often employed only to be dismissed and gaseous ones are rarely attested. In some cases, the shift from one set of metaphors to another can be shown to relate to broader changes in theoretical approaches.

CSIG-34. TYROSINE PHOSPHORYLATION OF NON-CANONICAL NF-ΚB P52 PROMOTES GLIOMA GROWTH AND CHEMORESISTANCE IN GBM

Abstract p52 is a nuclear factor-κB (NF-κB) transcription factor that comprises the non-canonical NF-κB pathway. The impact and mechanism of non-canonical NF-κB signaling remains understudied in glioblastoma (GBM). Subunit post-translational modification is a known mechanism of NF-κB activation, however phosphorylation of p52 has never been demonstrated. Elevated tyrosine kinase activity is a known-feature of GBM and portends worse survival. Further, p52 has been shown to upregulate factors that likely promote invasion of GBM, thereby reducing survival. Activation of p52 may serve as a link between increased kinase activity and poor survival in GBM. p52-knockout cell lines were generated using the CRISPR-Cas9 system and assessed for growth and survival differences in vitro and in syngeneic murine glioma models. Public glioma datasets and an institutional cohort were surveyed to corroborate p52-dependent survival differences in GBM. To determine whether p52 is post-translationally modified, we performed tandem mass spectrometry on p52 in GBM cells. Interacting factors were also analyzed and tested for site-specific modification of p52 via co-IP studies. We then generated PTM-null, knock-in glioma cell lines using CRISPR-CAS9 editing to demonstrate the functional significance of p52 PTMs, which were assessed using assays of relative cell growth, stemness, chemosensitivity and survival. Finally, we performed unbiased analysis of the p52 PTM-dependent transcriptome via RNA-seq. p52 loss significantly reduced glioma growth rate in vitro and improves murine survival. Retrospective analysis of GBM in patients suggests that p52 levels are negatively prognostic. Mass spectrometry identified multiple p52 PTMs with tyrosine phosphorylation sites as top hits. Identified phospho-tyrosine residues specifically interact with SRC-family kinases. PTM site editing significantly reduces glioma cell growth and stemness while enhancing chemosensitivity, reflecting broad transcriptome level changes. In sum, p52 activity enhances GBM growth and chemoresistance, which is partially modulated by tyrosine-specific phosphorylation. p52 warrants investigation as a therapeutic target in GBM, possibly in combination with kinase inhibitors and standard alkylating chemotherapy.

Climate warming detected in caves of the European Alps

Cave air temperatures in four caves in the European Alps show statistically significant warming trends of about 0.2 °C per decade over the last two decades (2000–2020). These trends are about half as large as those observed outside and are characterized by a remarkable spatial and temporal consistency. The investigated caves represent different types in terms of their ventilation regime and one of them also hosts perennial ice. Key observation sites are located in cave sections where the temporal variability of air temperature is strongly attenuated compared to outside conditions and data from different cave sections show that the main results are valid for large parts of the investigated caves. Continued warming will lead to broad changes in alpine cave environments, including changes in strength and direction of air flow in caves, karst hydrology and subsurface ecosystems. The observed subsurface warming has a particular strong effect on the long-term preservation of perennial ice present in some of these caves. This is shown for an ice cave in the Austrian Alps, where enhanced melt of ice correlates with the observed warming. This cave (and similar ones) will not be able to hold perennial ice beyond the next decade.

How Did Rituals Change with the Changing Ritual Places During the Second Millennium BC in Southwest England?

This study explores the transformation of ritual spaces in southwest England during the second millennium BC, focusing on the transition from monumental structures to residential settlements. Initially, ritual activities predominantly occurred within barrows and ring-cairns. By the Middle Bronze Age, however, these activities shifted towards houses and settlements, reflecting broader changes in architectural practices and social dynamics. This research examines the implications of these shifts through archaeological findings and literature review, highlighting how rituals became integrated into daily domestic spaces and the continuity of ritual practices through the reconfiguration of abandoned settlements into monument-like structures. The findings suggest that the evolution of ritual spaces was closely tied to the socio-spiritual landscape of the community, revealing significant insights into the cultural and social dynamics of prehistoric Europe.

Alternative polyadenylation shapes the molecular and clinical features of lung adenocarcinoma.

Alternative polyadenylation (APA) is a major mechanism of post-transcriptional regulation that affects mRNA stability, localization and translation efficiency. Previous pan-cancer studies have revealed that APA is frequently disrupted in cancer and is associated with patient outcomes. Yet, little is known about cancer type-specific APA alterations. Here, we integrated RNA-sequencing data from a Korean cohort (GEO: GSE40419) and The Cancer Genome Atlas (TCGA) to comprehensively analyze APA alterations in lung adenocarcinomas (LUADs). Comparing expression levels of core genes involved in polyadenylation, we find that overall, the set of 28 of 31 genes are upregulated, with CSTF2 particularly upregulated. We observed broad and recurrent APA changes in LUAD growth-promoting genes. In addition, we find enrichment of APA events in genes associated with known LUAD pathways and an increased heterogeneity in polyadenylation (polyA) site usage of proliferation-associated genes. Upon further investigation, we report smoking-specific APA changes are also highly relevant to LUAD development. Overall, our in-depth analysis reveals APA as an important driver for the molecular and clinical features of lung adenocarcinoma.

Proactive Monitoring of Changes in the Microbial Community Structure in Wastewater Treatment Bioreactors using Phospholipid Fatty Acid Analysis

Diverse microbial community structures (MCS) in wastewater treatment plants (WWTPs) are vital for effectively removing nutrients and chemicals from wastewater. However, the regular monitoring of MCS in WWTP bioreactors remains unattractive owing to the skill and cost required for deploying modern microbial molecular techniques in the routine assessment of engineered systems. In contrast, low-resolution methods for assessing broad changes in the MCS, such as phospholipid fatty acid (PLFA) analysis, have been used effectively in soil studies for decades. Despite using PLFA analysis in soil remediation studies to capture the long-term effects of environmental changes on MCS, its application in WWTPs, where the microbial mass is dynamic and operational conditions are more fluid, remains limited. In this study, microbial communities in a controlled pilot plant and 12 full-scale activated sludge plants (ASPs) were surveyed over a two-year period using PLFA analysis. This study revealed that changes in the MCS in wastewater bioreactors could be detected using PLFA analysis. The MCS comprised 59% Gram-negative and 9% Gram-positive bacteria, 31% fungi, and 1% actinomycetes. The abundances of Gram-negative bacteria and fungi were strongly inversely correlated, with an R2 = 0.93, while the fatty acids cy17:0 and 16:1ω7c positively correlated (R2 = 0.869). Variations in temperature, solid retention time, and WWTP configuration significantly influenced the MCS in activated sludge reactors. This study showed that WWTP bioreactors can be routinely monitored using PLFA analysis, and changes in the bioreactor profile that may indicate imminent bioreactor failure can be identified.

α-synuclein overexpression and the microbiome shape the gut and brain metabolome in mice

Pathological forms of α-synuclein contribute to synucleinopathies, including Parkinson’s disease (PD). Most cases of PD arise from gene-environment interactions. Microbiome composition is altered in PD, and gut bacteria are causal to symptoms in animal models. We quantitatively profiled nearly 630 metabolites in the gut, plasma, and brain of α-synuclein-overexpressing (ASO) mice, compared to wild-type (WT) animals, and comparing germ-free (GF) to specific pathogen-free (SPF) animals (n = 5 WT-SPF; n = 6 ASO-SPF; n = 6 WT-GF; n = 6 ASO-GF). Many differentially expressed metabolites in ASO mice are also dysregulated in human PD patients, including amine oxides, bile acids and indoles. The microbial metabolite trimethylamine N-oxide (TMAO) strongly correlates from the gut to the plasma to the brain in mice, notable since TMAO is elevated in the blood and cerebrospinal fluid of PD patients. These findings uncover broad metabolomic changes that are influenced by the intersection of host genetics and microbiome in a mouse model of PD.

(In)tangible Journeying and Its Transformation among the Rang from Byans, Far Western Nepal

The main inhabitants of Byans have traditionally undertaken seasonal migrations between their home villages and their winter residences in the southern hills of the Himalayas. Some have also been trans-Himalayan traders. Upon their death, their souls were traditionally guided from the Himalayan foothills in the south, through Chaudans and Byans, to their ancestral lands in the far north, by a long oral text called se yāmo. However, over the past seventy years, sociocultural transformation and the development of transport infrastructure have substantially transformed both their tangible and intangible journeyings. In this article, I demonstrate, through linguistic anthropological and ethnographic analyses, the interdependency between these two aspects of their journeying experience, and show how changes in the performance of posthumous rituals mirror the broader changes affecting their traditional journeying and movements across familiar landscapes.

Chronic polypharmacy, monotherapy, and deprescribing: Understanding complex effects on the hepatic proteome of aging mice.

Polypharmacy (use of ≥5 concurrent medications) is highly prevalent among older adults to manage chronic diseases and is linked to adverse geriatric outcomes, including physical and cognitive functional impairments, falls, frailty, hospitalization, and mortality. Deprescribing (withdrawal) is a potential strategy to manage polypharmacy. The broad molecular changes by which polypharmacy causes harm and deprescribing may be beneficial are unknown and unfeasible to study rigorously in tissue from geriatric patients. Therefore, in a randomized controlled trial, we administered therapeutic doses of commonly used chronic medications (oxycodone, oxybutynin, citalopram, simvastatin, or metoprolol) as monotherapy or concurrently (polypharmacy) from middle-age (12 months) to old-age (26 months) to male C57BL/6J (B6) mice and deprescribed (gradually withdrew) treatments in a subset from age 21 months. We compared drug-related hepatic effects by applying proteomics along with transcriptomics and histology. We found that monotherapy effects on hepatic proteomics were limited but significant changes were seen with polypharmacy (93% unique to polypharmacy). Polypharmacy altered the hepatic expression of proteins involved in immunity, and in drug, cholesterol, and amino acid metabolism, accompanied by higher serum drug levels than monotherapies. Deprescribing not only reversed some effects but also caused irreversible and novel changes in the hepatic proteome. Furthermore, our study identified several hepatic protein co-expressed modules that are associated with clinically relevant adverse geriatric outcomes, such as mobility, frailty, and activities of daily living. This study highlights the complex molecular changes following aging, chronic polypharmacy, and deprescribing. Further exploration of these mechanistic pathways may inform management of polypharmacy and deprescribing in older adults.

Generation Method for HVAC Systems Design Schemes in Office Buildings Based on Deep Graph Generative Models

The design process of heating, ventilation, and air conditioning (HVAC) systems is complex and time consuming due to the need to follow design codes. Since the design standards are not fixed, the final outcome often depends on the designer’s experience. The development of building information modeling (BIM) technology has made information throughout the building lifecycle more integrated. BIM-based forward design is now widely used, providing a data foundation for combining HVAC system design with machine learning. This paper proposes an unsupervised learning method based on deep graph generative models to uncover hidden design patterns and optimization strategies from the design results. We trained and validated four deep graph generative models—GAE, GNF, GAN, and diffusion—using HVAC system terminal pipeline layout data. Accuracy and precision metrics were used to compare the generated designs with automated forward design solutions, assessing the models’ ability to capture both local variations and broader changes in design logic. A graph-neural-network-based evaluation method was employed to measure the models’ capacity to detect changes. The results indicate that all four models achieved prediction accuracies exceeding 90% and precision rates above 75%. The models effectively captured both local modifications made by designers and global design changes, showing greater sensitivity to global layout adjustments than to local updates. When comparing the results generated by deep graph generative models and the actual design, it is obvious that the accuracy of the predictions varies significantly due to the complexity of the test buildings.

From ‘Practical Application of Knowledge’ to ‘Academic Self-Awareness’: A Historical Examination of Knowledge Production in Chinese Middle East Studies

The knowledge production in Chinese Middle East studies boasts a long history. The article traces the evolution of Chinese scholarship on Middle East studies from ancient times to the present. Initially driven by the practical needs for imperial expansion and foreign trade, this scholarship focused on the academic tradition of the ‘practical application of knowledge.’ Over time, it evolved into a phase of academic self-awareness, characterized by a more systematic and independent approach to Middle East studies. This shift reflects broader changes in China’s foreign relations and academic priorities, with contemporary scholars emphasizing the development of an autonomous knowledge production system through various models, such as major-theme research, discipline-driven research, and integrated research. The article suggests that future research should adopt an ‘issue-driven’ model to enhance further the depth and autonomy of Chinese Middle East studies.

Electrical Resistivity Tomography and Boreholes Data to Investigate the Near-Surface Structure under the Campus Area of Çanakkale Onsekiz Mart University, Çanakkale, Turkey (Türkiye)

We study the soil foundation underneath the Çanakkale Onsekiz Mart University (ÇOMU) campus, Çanakkale, Türkiye by employing the electrical resistivity tomography – ERT supported by 27 boreholes data. The studied area taking place in southwest Marmara region was historically affected by large earthquakes () created by the North Anatolian Fault system. The boreholes data show that the near surface structure beneath the ÇOMU campus is made of mostly silty sands and marls. A high sensitivity resistivity instrument is used to collect the field data in which nine ERT profiles reaching lengths as long as 315 m are utilized. The current geoelectrical measurements are simulated by using two numerical models to estimate the inversion depth sensitivity from which it is found satisfactory in the depth range 0-30 m and then somewhat decreasing. The observed electrical resistivity values are in the range 2-160 W m. The geoelectrical structure corresponding to the silty sands are represented by low resistivities (<20 W m) while the high resistivity (>40 W m) depth sections are associated with the marl units. The resistivity structure beneath the ÇOMU campus is complex where both low and high resistivity depth sections reside side by side. The groundwater and clay mineralogy contribute to the broad changes in the subsurface resistivities. The groundwater flow below the steep terrain of the ÇOMU campus causes low resistivities (<10 W m) deeper than 10-m depth. The boreholes data superimposed on the two-dimensional (2-D) ERT profiles show consistency with the resistivity-depth distributions at corresponding depths.

Motor-Related EEG Analysis Using a Pole Tracking Approach.

This study introduces an alternative approach to electroencephalography (EEG) time-frequency analysis based on time-varying autoregressive (TV-AR) models in a cascade configuration to independently monitor key EEG spectral components. The method is evaluated for its neurophysiological interpretation and effectiveness in motor-related brain-computer interface (BCI) applications. Specifically, we assess the ability of the tracked EEG poles to discriminate between rest, movement execution (ME) and movement imagination (MI) in healthy subjects, as well as movement attempts (MA) in individuals with spinal cord injury (SCI). Our results show that pole tracking effectively captures broad changes in EEG dynamics, such as transitions between rest and movement-related states. It outperformed traditional EEG-based features, increasing detection accuracy for ME by an average of 4.1% (with individual improvements reaching as high as 15%) and MI by an average of 4.5% (up to 13.8%) compared to time-domain low-frequency EEG features. Similarly, compared to alpha/beta band power, the method improved ME detection by an average of 5.9% (up to 10.4%) and MI by an average of 4.3% (up to 10.2%), with results averaged across 15 healthy participants. In one participant with SCI, pole tracking improved MA detection by 12.9% over low-frequency EEG features and 4.8% over alpha/beta band power. However, its ability to distinguish finer movement details within specific movement types was limited. Additionally, the temporal evolution of the extracted pole tracking features revealed event-related desynchronization phenomena, typically observed during ME, MA and MI, as well as increases in frequency, which are of neurophysiological interest.

“Girls’ things” make headlines: menstruation in the media coverage of women’s tennis

ABSTRACT The aim of this article is to analyze the news media discourse around menstruation in women’s sport by examining news media coverage of two tennis players, Heather Watson and Qinwen Zheng, who attributed their defeat in 2015 and 2022, respectively, to “girls’ things.” Using thematic analysis, the article analyses four themes: (1) breaking the menstrual taboo in sport, (2) specificity and marginalization of the female body, (3) the risk of a leaking body and (4) the wider context beyond the world of sport. The analyzed discourse is part of the broader changes observed since the mid-2010s regarding the greater presence of menstruation in public discourse. The most significant difference between the 2015 and 2022 corpora lies in the framing of menstruation as taboo. Whereas an open statement about menstruation in 2015 was perceived as something both brave and shocking, such discourse was practically absent in 2022. Despite this change, this discourse illustrates the tension between the public exposure of menstruation alongside efforts aimed at keeping it hidden. It can be read in the framework of neoliberal feminism by emphasizing the privileging of the male body and the current product-focused menstrual activism by highlighting the need to keep menstruation invisible.

DELE1 maintains muscle proteostasis to promote growth and survival in mitochondrial myopathy.

Mitochondrial dysfunction causes devastating disorders, including mitochondrial myopathy, but how muscle senses and adapts to mitochondrial dysfunction is not well understood. Here, we used diverse mouse models of mitochondrial myopathy to show that the signal for mitochondrial dysfunction originates within mitochondria. The mitochondrial proteins OMA1 and DELE1 sensed disruption of the inner mitochondrial membrane and, in response, activated the mitochondrial integrated stress response (mt-ISR) to increase the building blocks for protein synthesis. In the absence of the mt-ISR, protein synthesis in muscle was dysregulated causing protein misfolding, and mice with early-onset mitochondrial myopathy failed to grow and survive. The mt-ISR was similar following disruptions in mtDNA maintenance (Tfam knockout) and mitochondrial protein misfolding (CHCHD10 G58R and S59L knockin) but heterogenous among mitochondria-rich tissues, with broad gene expression changes observed in heart and skeletal muscle and limited changes observed in liver and brown adipose tissue. Taken together, our findings identify that the DELE1 mt-ISR mediates a similar response to diverse forms of mitochondrial stress and is critical for maintaining growth and survival in early-onset mitochondrial myopathy.

6813 Des-Ciclesonide: A Novel Selective Glucocorticoid Receptor Modulator With Therapeutic Potential for Bronchopulmonary Dysplasia, a Chronic Lung Disease of Prematurity

Abstract Disclosure: N. El Khoury: None. J. Jaumotte: None. C. Min: None. C. Madigan: None. J. Wang: None. U. Chandran: None. V. Sampath: None. T.J. Cole: None. P. Monaghan-Nichols: None. R. Houtman: None. K.W. Nettles: None. D.B. DeFranco: None. Infants born before 30 weeks gestation are at risk of developing bronchopulmonary dysplasia (BPD), a prevalent chronic lung disease of prematurity. Glucocorticoids (GCs) such as dexamethasone (DEX) have been a mainstay of BPD prevention as they reduce lung inflammation and injury. However, their use is encumbered with several adverse effects including hyperglycemia, impaired somatic and brain growth and cerebral palsy. These major concerns have resulted in the AAP recommending caution in GC use in preterm infants.Ciclesonide (CIC), an inhaled GC prodrug approved for the treatment of asthma and allergic rhinitis in children, is converted to the potent glucocorticoid receptor (GR) agonist des-ciclesonide (DES) by enzymes enriched in the airways. We previously reported that s.c. delivery of CIC in neonatal rats activated GR transcriptional responses in lung but did not trigger the adverse effects on somatic growth, brain weight or cerebral white matter loss caused by DEX leading us to propose CIC as a potential new pharmacotherapy for BPD. To determine whether limited systemic metabolism of CIC in neonatal rats was solely responsible for its enhanced safety profile, we assessed various outcomes in neonatal rats followed s.c. delivery of its active metabolite DES. Consistent with previous studies, five daily s.c. injections of DEX led to a reduction in body and brain weight as well as reduced serum levels of insulin-like growth factor-1 and chronic hyperglycemia. Surprisingly, analogous s.c. injection of neonatal rats with DES, which has a higher binding affinity for GR than DEX, did not trigger any of these adverse effects. However, DES is as effective as DEX in reducing the expression of various proinflammatory cytokine mRNAs in neonatal rat lung induced by an 11-day systemic treatment with bleomycin. Bulk RNAseq analyses revealed transcriptional responses of neonatal lung and liver to DES, although less robust than that observed to DEX (i.e., 25% and 13% in lung and liver, respectively). Nonetheless, a NAPing assay for coregulator peptide binding showed increased binding of all the DES responsive peptide interactions relative to DEX and cortisol. All-atom molecular dynamics simulations revealed site-specific H-bonding changes within the GR ligand binding domain (LBD) caused by the bulky C16/C17substitution within DES, which was translated into broader changes in LBD structure, specifically revealed as differences in RMSF in helix 7/8 and the surfaces interacting with the NCOA2 peptide. Furthermore, network pathway analysis illustrated that DES mobility at helix 7/8 uniquely generated a meaningful allosteric signaling pathway between Q642 and NCOA2 peptide. In conclusion, the pharmacokinetic properties of the GR prodrug CIC coupled with the tissue-selective pharmacodynamic properties of its active metabolite DES make this FDA approved drug a potential new therapeutic agent for BPD. Presentation: 6/3/2024

Identity Struggle and Ethno-Social Conflict in Papua: Uncovering the Root of the Problem and Finding Sustainable Solutions

Background: Papua Province is a region rich in diverse ethnicities and has complex social dynamics due to significant ethnic differences. Social conflicts in Papua are not only conflicts between individuals, but also reflect broader changes with cultural, political and socio-economic aspects.Objective: The purpose of this study is to examine and analyze the determinants of social conflict in Papua based on ethnicity and to propose strategies for achieving sustainable social harmony.Method: This research employs a qualitative method focusing on indigenous Papuans, selecting informants such as traditional leaders, community leaders, youth, women, religious figures, and bureaucrats through in-depth interviews and literature studies conducted from July to August 2023.Results: Papuan ethnic identity is shaped by their unique cultural values and traditions, distinguishing them from other ethnic groups in Papua. However, the government's development programs fail to address this identity, contributing to the marginalization of Papuans.Conclusion: The conflict in Papua stems from Papuans fighting for their basic rights tied to their ethnic identity, rather than economic or political issues, highlighting the need for future research on development policies that respect this identity.

Deconstruction the feedforward inhibition changes in the layer III of anterior cingulate cortex after peripheral nerve injury

The anterior cingulate cortex (ACC) is one of the critical brain areas for processing noxious information. Previous studies showed that peripheral nerve injury induced broad changes in the ACC, contributing to pain hypersensitivity. The neurons in layer 3 (L3) of the ACC receive the inputs from the mediodorsal thalamus (MD) and form the feedforward inhibition (FFI) microcircuits. The effects of peripheral nerve injury on the MD-driven FFI in L3 of ACC are unknown. In our study, we record the enhanced excitatory synaptic transmissions from the MD to L3 of the ACC in mice with common peroneal nerve ligation, affecting FFI. Chemogenetically activating the MD-to-ACC projections induces pain sensitivity and place aversion in naive mice. Furthermore, chemogenetically inactivating MD-to-ACC projections decreases pain sensitivity and promotes place preference in nerve-injured mice. Our results indicate that the peripheral nerve injury changes the MD-to-ACC projections, contributing to pain hypersensitivity and aversion.

Nuclear Type I Myosins are Essential for Life and Genome Organization.

The active transport of large biomolecules within a cell is critical for homeostasis. While the cytoplasmic process is well-studied, how the spacing of nucleoplasmic cargo is coordinated is poorly understood. We investigated the impact of myosin motors in the nucleus of budding yeast. We found that life requires a nuclear type I myosin whereas the essential type II or V myosins were not requisite in the nucleus. Nuclear depletion of type I myosins triggered 3D genome disorganization, nucleolar disruption, broad gene expression changes, and nuclear membrane morphology collapse. Genome disorganization occurred first supporting a model where type I myosins actively maintain genome architecture that scaffolds nuclear membrane and nucleolar morphologies. Overall, nuclear myosin is critical for the form and function of the nucleus.