Brackish Water Ponds Research Articles

Mud crab, Scylla tranquebarica (Fabricius, 1798), crablet grow‐out in lined ponds with different stocking densities to support soft‐shell crab production

AbstractCrab stocking densities in brackish water ponds can influence crab growth rate, survival, and production volume. This study aimed to determine the optimum grow‐out stocking density for Scylla tranquebarica crablets reared in lined ponds to supply the soft‐shell crab industry. Three stocking density treatments (1, 4, and 8 ind./m2) were applied to crablets reared in lined ponds for 90 days. The crablets were fed low‐value fish (Leiognathus equula) at an initial rate of 100% of total body weight for two weeks, reduced to 3% toward the end of the study. Crablets stocked at 1 and 4 ind./m2 had a significantly (p < 0.05) higher net growth (NG) and daily growth rate (DGR) than crablets stocked at 8 ind./m2. Deformed crabs were also most common at the highest stocking density. The intermediate stocking rate achieved moderate survival and feed conversion rates, with the highest crab production volume (19.65 ± 0.10 kg/100 m2) and net income (6845.60 US$/Ha/2 crops/year). Based on these findings, a stocking rate of around 4 ind./m2 is recommended for the grow‐out of S. tranquebarica crablets in lined ponds.

Soil remediation and nano-biosilica: a potential combination to improve the environmental quality of brackishwater aquaculture ponds affected by acid sulfate soils.

The successful management of ASS-affected brackishwater aquaculture ponds necessitates overcoming associated environmental limitations. This study investigated the potential application of nano-biosilica from rice husk ash (RHA) and soil remediation techniques to improve the environmental quality of ASS-affected brackishwater ponds. The study followed a completely randomized design (CRD) with four treatments and three replicates. The treatments comprised applying soil remediation, nano-biosilica fertilizer, and their combination. The study generally revealed that the combination of soil remediation technique and RHA-driven nano-biosilica improved the water quality of ASS-affected brackishwater ponds. Soil remediation improved water quality by reducing acidity levels. However, excessive lime application as an integral part of the remediation might release acidity and toxic metals into water, potentially increasing calcium-phosphorus fixation. Despite liming potential negative consequences, if mixed with nano-biosilica could increase diatom-phytoplankton growth by reducing dissolved Al and Fe levels while boosting P and Si availability. Liming could also help boost diatom photosynthesis and inhibit unwanted algae blooms by decreasing water turbidity and increasing sunlight penetration. This study emphasized that the effectiveness of nano-biosilica in promoting diatom growth depends on appropriate nitrogen (N) and phosphorus (P) concentrations and ratios, which should not be a limiting factor. However, the required N/P concentration and ratio are only met if the remediation method is effectively implemented. The combination of nano-biosilica and soil remediation treatment maintained SiO2 concentrations above the average natural seawater concentration; however, availability may be limited due to complexes containing Ca, Al, Mg, and Fe. Regularly applying cost-effective nano-biosilica fertilizer in combination with N and P fertilizers is recommended to enhance water remediation efficiency by boosting Si availability and decreasing the toxicity of dissolved toxic metal ions.

Identifying the Environmental Factors that Determine the Agar Content of Seaweed <i>Gracilaria verrucosa</i> in Acid Sulfate Soil-Affected Brackishwater Ponds

Identifying the Environmental Factors that Determine the Agar Content of Seaweed <i>Gracilaria verrucosa</i> in Acid Sulfate Soil-Affected Brackishwater Ponds

Integrative data of a novel ciliate (Alveolata, Ciliophora) propose the establishment of Heterodeviata nantongensis nov. sp.

BackgroundAs unicellular eukaryotes, ciliates are an indispensable component of micro-ecosystems that play the role of intermediate nutrition link between bacteria or algae and meiofauna. Recent faunistic studies have revealed many new taxa of hypotrich ciliates, indicating their diversity is greater than previously thought. Here we document an undescribed form isolated from an artificial brackish water pond in East China. Examination of its morphology, ontogenesis and molecular phylogeny suggests that it represents a new species.ResultsThe morphology and morphogenesis of the new brackish-water deviatid ciliate, Heterodeviata nantongensis nov. sp., isolated from Nantong, China, were investigated using live observations and protargol staining. The diagnostic traits of the new species include three frontal cirri, one buccal cirrus, one or two parabuccal cirri, an inconspicuous frontoventral cirral row of four to six frontoventral cirri derived from two anlagen, three left and two right marginal rows, two dorsal kineties, dorsal kinety 1 with 9–14 dikinetids and dorsal kinety 2 with only two dikinetids, and one to three caudal cirri at the rear end of dorsal kinety 1. Its main morphogenetic features are: (i) the old oral apparatus is completely inherited by the proter except undulating membranes, which are reorganized in situ; (ii) anlagen for marginal rows and the left dorsal kinety develop intrakinetally in both proter and opisthe; (iii) dorsal kinety 2 is generated dorsomarginally; (iv) five cirral anlagen are formed in both proter and opisthe; (v) in the proter, anlagen I and II very likely originate from the parental undulating membranes and the buccal cirrus, respectively, anlage III from anterior parabuccal cirrus, anlage IV originates from the parental frontoventral cirri and anlage V from the innermost parental right marginal row; and (vi) anlagen I–IV of the opisthe are all generated from oral primordium, anlage V from the innermost parental right marginal row. Phylogenetic analyses based on SSU rRNA gene sequence data were performed to determine the systematic position of the new taxon.ConclusionsThe study on the morphology, and ontogenesis of a new brackish-water taxon increases the overall knowledge about the biodiversity of this ciliate group. It also adds to the genetic data available and further provides a reliable reference for environmental monitoring and resource investigations.

Philippine mangroves: species composition, characteristics, diversity, and present status

Through the years mangrove areas in the Philippines continued to decline due to human activities. This paper aims to present the status of the Philippine mangroves based on species composition, diversity, and mangrove forest area through manuals, books, and other local and international online resources. Results showed that there were 35 species of mangroves described in terms of their external features, substrate, and zone preference. Bohol had the most diverse mangrove ecosystem with 26–34 species. The most prevalent species were Avicennia, Sonneratia, and Rhizophora that grew in muddy substrates. Among the provinces, 18.52% had mangrove areas recognized as protected areas. Mangrove decline is attributed to the increase of brackish water culture ponds and the greatest decline occurred when shrimp culture boomed in the 1980s. Other causes are over exploitation, weak law enforcement, lack of manpower and resources, corruption, and poor management of mangrove areas and brackish water ponds. Along with this is the loss of flood control and coastal protection indicating increased susceptibility to climate change. Thus, reforestation and reversion of abandoned brackish water ponds are needed. Reforestation must ensure the suitability of mangrove species to the physical characteristics of the environment. Also, the government must actively spearhead the sustainable use of mangrove ecosystems.

Simultaneous aerobic nitrogen and phosphate removal capability of novel salt-tolerant strain, Pseudomonas mendocina A4: Characterization, mechanism and application potential

A salt-tolerant strain, Pseudomonas mendocina A4, was isolated from brackish-water ponds showing simultaneous heterotrophic nitrification-aerobic denitrification and phosphorus removal capability. The optimal conditions for nitrogen and phosphate removal of strain A4 were pH 7–8, carbon/nitrogen ratio 10, phosphorus/nitrogen ratio 0.2, temperature 30 °C, and salinity range of 0–5 % using sodium succinate as the carbon source. The nitrogen and phosphate removal efficiencies were 96–100 % and 88–96 % within 24 h, respectively. The nitrogen and phosphate removal processes were matched with the modified Gompertz model, and the underlying mechanisms were confirmed by the activities of key metabolic enzymes. Under 10 % salinity, the immobilization technology was employed to enhance the nitrogen and phosphate removal efficiencies of strain A4, achieving 87 % and 76 %, respectively. These findings highlight the potential application of strain A4 in both freshwater and marine culture wastewater treatment.

Characteristics of the aquatic environment as a basis for brackish water pond development in the Padang Pariaman regency area, Indonesia

The coast of Padang Pariaman Regency is one of the shrimp productions centra area in West Sumatera Province. This study aims to show water conditions in aquaculture areas for vanname shrimp pond. Carried out every month through insitu and exsitu water sampling and analysis in 6 locations from May-July 2022. Observations were made on water physical-chemistry parameters (temperature, pH, salinity, dissolved oxygen-DO, COD, ammonia, TSS and phosphate). Data were analyzed descriptively, compared with environmental quality standards for biota based on decree of Government Number 22 of 2021. Based on the analysis, it is known that the range (and standard deviation) of water quality varies, namely temperature 26.7-33.5 °C (29.79±1.93 °C), pH 7.37-8.8 (7.86±0.31), salinity 25-36 ‰ (29.83±2.94 ‰), DO 6.01-8.90 mg/l (7.70±0.99 mg/l), ammonia 0.01-3.25 mg/l (1.65±0.91 mg/l), phosphate 0.03-0.33 mg/l (0.18±0.09 mg/l), TSS 2.03-14.46 mg/l (4.49±3.32 mg/l), and COD 17.6-88.0 mg/l (54.76± 21.25 mg/l). Based on the observations, it can be concluded with the condition of the brackish pond waters is good for the development of vanname shrimp aquaculture.

FEASIBILITY OF CULTIVATING MILKFISH WITH A NET CAGE SYSTEM, API-API VILLAGE, WONOKERTO DISTRICT, PEKALONGAN REGENCY

Api-Api Village has potential fishery resources, especially milkfish cultivation, but milkfish cultivation has obstacles and challenges due to sea tidal disasters and other things. Farmers continue to exploit the existing potential by cultivating the stepped net cage system. The objectives of this research were (1) to determine the general condition of brackish water ponds in Api-Api village and (2) to analyze the financial feasibility of cultivating milkfish using the stepped net cage system. The data collection method used was direct interviews with 30 farmers. The analytical tool used is financial feasibility analysis (NPV, Net B/C, IRR, PP). The results of the financial feasibility analysis show an NPV value of Rp. 71,838,127, Net B/C of 1.64, IRR of 15.33% and Payback Period of 2 years seven months 17 days. Therefore, cultivating milkfish using the KJT system in Api-Api Village is feasible.

Characteristics of the aquatic environment as a basis for brackish water pond development in the Cilacap Regency Area

The coastal of Cilacap is a large aquaculture area for vannamei shrimp (Litopenaeus vannamei). The purpose of this study is to determine the concentration of water quality in borewell and pond as the basis for brackish water development. The research method used a descriptive method by describing the sample results compared to environmental quality standards for biota based on Government Regulation Number 22 of 2021. Sampling was carried out monthly during May-July 2022 at 3 location. Observations were made on water physico-chemical parameters (temperature, pH, salinity, dissolved oxygen, BOD, nitrite, nitrate, ammonia and phosphate). Based on the analysis, it is known that the water quality of the borewell varied, namely temperature (29.75±0.96°C), pH (7.74±0.26), salinity (15±6.42%o), DO (6.52±2.83) mg/l, BOD (2.48.±1.67) mg/l, nitrate (0.43±0.38) mg/l, nitrite (0.02±0.02) mg/l, ammonia (0.46±0.30) mg/l and phosphate (0.13±0.10) mg/l. As for the pond water quality parameters, the temperature values were (30.14±1.46°C), pH (7.94±0.22), salinity (12.22±6.02%o), DO (7.58±1.30) mg/l, BOD (26.10±24.83) mg/l, nitrate (1.86±1.30) mg/l, nitrite (0.09±0.15) mg/l, ammonia (1.07±0.30) mg/l and phosphate (0.128±0.098) mg/l. Based on Government Regulation Number 22 of 2021 it is concluded that the water conditions are still suitable for the life of aquatic biota and good enough for fishery development.

Halophyte and bivalve-based integrated multi-trophic aquaculture (IMTA): effect on growth, water quality, digestive and antioxidant enzymes of Penaeus monodon and Chanos chanos reared in brackishwater ponds

Halophyte and bivalve-based integrated multi-trophic aquaculture (IMTA): effect on growth, water quality, digestive and antioxidant enzymes of Penaeus monodon and Chanos chanos reared in brackishwater ponds

Effects of stocking densities on tilapia seed production under high salinity biofloc system

Provision of suitable technique for saline tilapia seed is required since demand of these species increased continuously. Most saline tilapia seed rely on sourcing freshwater fingerling and further acclimatized in brackishwater ponds. The study aimed to evaluate the effects of various fry densities on seed production parameters of tilapia under high salinity-biofloc system. Three fry densities being tested and cultured under biofloc technology (BFT) at salinity level above 20 ppt, such as: A (BFT 2.5 fry L−), B (BFT 5.0 fry L−), C (BFT 7.5 fry L−) and one treatment as control, D (Non-BFT 2.5 fry L−). The experimental set-up was completely random design and triplicated replications. Animals test were fed two times a day of 32% crude protein content and it was lasted for three weeks. Results from the experiment shown that treatment A (BFT 2.5 fry L−) performed best in term of biomass (18.00±3.61g), survival (98.00±2.00%), SGR (20.72±0.20% d−), FCR (1.72±0.38) and CF (1.59±0.17). An increasing stocking density up 7.5 fry L− will reduced survival rate, final individual weight and increased FCR. It can be concluded that production of tilapia seed at a density of 2.5 fry L− under BFT conditions performed healthy seed and best production parameters even reared at higher salinity.

Survival and Immune Responses of F1 Transgenic Tiger Shrimp Penaeus monodon Against White Spot Syndrome Virus (WSSV)

Transgenic technology has been applied to tiger shrimp Peneus monodon to produce a resistant strain to white spot syndrome virus (WSSV) by antiviral gene overexpression. The founders (F0) transgenic tiger shrimp have been successfully bred to produce the first generation (F1). The present study aimed to evaluate the survival and immune responses of F1 transgenic tiger shrimp against WSSV. The F1 transgenic and non-transgenic shrimp (control) with an average weight of 7.93±1.49 g were collected from the controlled brackish water ponds and stocked in a 20 L fiberglass tank with a density of 5 shrimp/tank. The transgenic shrimps were confirmed by a PCR assay. The shrimp were intramuscularly injected with WSSV to evaluate the performance between transgenic and non-transgenic. The survival (SR) was observed daily after the challenge test. Measurement of immune responses, namely total haemocyte count (THC), differential haemocyte count (DHC), prophenoloxidase (proPO) activity, and RNA content in haemolymph was conducted on before challenge, and on the 1st, 3rd, and 5th days after challenge. The survival and immune responses were statistically analysed by the t-student test. The results showed that the SR of transgenic (52.0%), with a relative percentage survival of 47.82%, was greater (P<0.05) than the control shrimp (8.0%). The THC, proPO, and RNA content of the transgenic shrimp was higher (P<0.05) than the non-transgenic shrimp. The results suggested that the transgenic tiger shrimp, due to antiviral gene overexpression, increased the resistance against WSSV.

Evaluation of ammonium issues in Indonesian groundwater: Potential sources and removal methods

Elevated concentration of ammonium (NH4 +) has been identified as one of the problems with groundwater because it indicates anthropogenic disturbance. In this study, we evaluated the NH4 + presence in Indonesian groundwater and the land-use conditions possibly related to the issues. In addition, this research presents practical strategies for reducing NH4 + concentrations in groundwater. The spatial distribution shows that high levels of NH4 + are found in densely populated areas. Furthermore, land-uses analysis reveals that considerable concentrations of NH4 + were found in several land-uses, including settlement, agricultural, fishpond, industrial, and garbage disposal, reaching 24.16, 7.73, 16.7, 7.42, and 84.9 mg/L, respectively. However, the statistical analysis shows that not all land-uses types significantly correlated with groundwater NH4 + contents. Based on the p-value and confidence interval of 95%, NH4 + concentrations are significantly correlated with settlements, brackishwater ponds, and garbage disposal. Similarly, industrial and agricultural land-uses have positive correlations with NH4 + concentrations, although these correlations are insignificant. Finally, the promising methods for NH4 + removal are the adsorption and ion exchange methods using zeolite or biochar, which could reduce NH4 + levels by 90% and more. Furthermore, these methods do not require complicated application procedures.

Lime application to condition soil to pH 11 controls growth of harmful vibrios and WSSV

Shrimp farming has been devastated by several diseases. Disease outbreaks can be prevented by optimising environmental conditions or by eliminating risks factors including carrier organisms. These can be achieved through proper pond preparation. One important step in pond preparation is the application of hydrated lime. This paper describes the effect of hydrated lime application to condition pond soil to pH 11 on the pond ecosystem, more specifically on soil pH and biota. The experiment was done using three 600 m2 brackish water ponds. After hydrated lime application, the wet soil pH on the 1st-8th day was significantly higher (P<0.05) compared to the pH before liming to pH 11, and flushing after flooding for 24 h. Soil pH of 11 was observed 24-48h after lime application. Moreover, unwanted species like crabs and fish were found dead. Liming to pH 11 negatively affects the counts of green vibrio colonies thereby decreasing the percentage of green vibrios (PGV). In another experiment, three 750m2 earthen ponds were used. The pond soils were previously positive for the Whitespot Syndrome Virus (WSSV), hydrated lime were applied to bring the pond soil pH to 11. After lime application, WSSV was not detected in the pond soils. Results showed that increasing the soil pH to ≥11 positively affects the pond ecosystem. Liming encourages the proliferation of yellow vibrios and the eradication of carrier species (i.e. crabs) as well as predators (i.e. fish) in the ponds. Furthermore, liming to pH 11 may also eradicate White spot virus from the pond sediment. To implement a 1-unit increase in wet pH, hydrated lime should be applied at 2T/ha.

Role of Natural Food in Enhancing the Productivity of Saline Nile Tilapia in the Mangrove Ecosystem of Segara Anakan Lagoon, Brackish Water Culture

Saline Nile Tilapia, Oreochromis niloticus (Linnaeus, 1758) and Milkfish, Chanos chanos (Forskal, 1775) polyculture systems in brackish water culture require natural food to sustain their brackish water fish production. Brackish water culture of Saline Nile Tilapia is developed to improve the productivity of abandoned shrimp or crab pond. At present, there are no studies examining the potential of natural food to improve the productivity of these polyculture systems in the brackish water pond located in Tritih Kulon Village, Cilacap, Central Java. To assess the availability of this food source, the diversity and abundance of plankton in the water body were evaluated through analysis. Therefore, this study aimed to analyze plankton diversity in the water body and the relationship between plankton abundance as a natural feed and saline Nile Tilapia productivity in the brackish water polyculture. Phytoplankton and zooplankton were identified according the plankton characteristic available in the references. The results showed that 21 planktons, comprising 12 phytoplankton species and 9 zooplankton groups, were identified. In spite of low plankton diversity, however, this study proved that the plankton abundance positively supported Saline Nile tilapia productivity in the polyculture system with milkfish, as indicated by low mortality (17,5 ±8,59 %; R2= 0.825-0.908), absolute high weight gain (208.2 ± 22,5 gr; R2= 0.881-0.874), and high specific growth (2,28 ± 0.77 % day-1; R2= 0.87-0.91). The productivity of Saline Nile Tilapia in brackish water polyculture with milkfish is supported by the availability of natural food, with 5.95 to 18.50% of their gut content obtained from plankton.

Microplastics contaminant in Telescopium telescopium (gastropods), the keystone mangrove species and their habitat at brackish water pond, East Java, Indonesia

Microplastics (MPs) have become a compelling topic of discussion, particularly after numerous studies reported their presence in aquatic ecosystems, which can contaminate pond areas directly connected to water. Therefore, this study aimed to identify the abundance and the characteristics of MPs in surface water, sediment, and T. telescopium (Gastropods) in pond areas. The samples were obtained from three site categories, namely rivers, canals, and ponds, located in the Pasuruan pond areas, East Java, Indonesia. After an optimized extraction protocol, a stereomicroscope and Fourier transform infrared spectroscopy (FTIR) spectroscopic imaging was used to identify MPs polymer. The results showed that the average MPs abundance in the three site categories were 33.87 ± 8.95, 21.73 ± 10.28, and 3.77 ± 1.90 particles/L at surface water, 404.43 ± 101.18, 328.88 ± 98.8, and 317.77 ± 100.07 particles/kg dry weight (dw) at sediment and 5.7 ± 5.03, 5.8 ± 5.27, and 4.4 ± 3.86 particles/individual or 0.8 ± 0.7, 0.8 ± 0.8, and 0.63 ± 0.51 particles/g wet weight (ww) at T. telescopium. The forms found were fragments, fibers, films, and pellets, while the dominant colors were black, blue, red, and white, and the highest amounts were <300 μm in size. Subsequently, the polymer types identified were Low-Density Polyethylene (LDPE), Ethylene Vinyl Acetate (EVA), Latex, High-Density Polyethylene (HDPE), Polypropylene (PP), Polyvinyl Chloride (PVC), Polycarbonate, and Polystyrene (PS). Further study is needed to understand MPs impact in brackish water aquaculture and the role of T. telescopium as a MPs bioindicator agent in aquatic ecosystems.

Mud Crab Culture in the Coastal Brackish Water Wetlands of West Bengal – A Promising Enterprise

Mud crab culture or crab fattening in brackish water wetlands of coastal West Bengal locally known as Chamber chas was initiated in the Sundarban coast since 1990s. Two species, viz., Scylla serrata and Scylla tranquebarica are reared in small and medium sized brackish water ponds for a period of about 18 days, using undersized and discarded crabs, mainly with help of household manpower to earn their living in this region. This is briefly reviewed herein to highlight the crab fattening enterprise and to promote the mud crab aquaculture so that the dependency on capture fishing may be reduced especially from mangrove tigerland of Sundarban, West Bengal.

The Effect of Use Herbal Plus Probiotic on Nursery Stage Milkfish (Chanos Chanos) Culture

The milkfish, or Chanos chanos, is a fish that is frequently raised in brackish water ponds and is in high demand by consumers. It has a large economic value. A procedure known as nursery cultivation is used to maintain milkfish fry and produces fish that are prepared to be reared in ponds when they reach a specific size. The goal of this study is to determine how probiotics and herbs affect the growth and survival of milkfish. The materials used in this study were milkfish with an average weight of 0.018−0.01 g, commercial milkfish feed with a 20% protein content, probiotics, and a mixture of turmeric extract and curcuma. The investigation was conducted utilizing an experimental technique and a thoroughly randomized design with three replications of each treatment and one control. Data analysis used analysis of variance (One Way Anova) to compare milkfish cultivation in treatment A (milkfish with herbal feed), treatment B (milkfish with feed containing probiotics), treatment C (milkfish with feed containing herbs and probiotics), and control (milkfish nursery with feed). The analysis’s results showed that while adding herbs, probiotics, or herbs plus probiotics to the diet had no significant effects on milkfish survival (P > 0.05), they did significantly influence fish growth when compared to controls (P 0.05). When compared to controls, W herbs + probiotics in feed had a substantial impact on fish development but no significant impact (P>0.05) on milkfish survival. When compared to controls, W herbs + probiotics in feed had a substantial impact on fish development but no significant impact (P>0.05) on milkfish survival. During the maintenance period, the water quality metrics were within an acceptable range for milkfish to survive and thrive..

Enrichment of hydrogen-oxidizing bacteria using a hybrid biological-inorganic system.

Hybrid biological-inorganic (HBI) systems comprising inorganic water-splitting catalysts and aerobic hydrogen-oxidizing bacteria (HOB) have previously been used for CO2 conversion. In order to identify new biocatalysts for CO2 conversion, the present study used an HBI system to enrich HOB directly from environmental samples. Three sediment samples (from a brackish water pond, a beach, and a tide pool) and two activated sludge samples (from two separate sewage plants) were inoculated into HBI systems using a cobalt phosphorus (Co-P) alloy and cobalt phosphate (CoPi) as inorganic catalysts with a fixed voltage of 2.0V. The gas composition of the reactor headspaces and electric current were monitored. An aliquot of the reactor medium was transferred to a new reactor when significant consumption of H2 and CO2 was detected. This process was repeated twice (with three reactors in operation for each sample) to enrich HOB. Increased biomass concomitant with increased H2 and CO2 consumption was observed in the third reactor, indicating enrichment of HOB. 16S rRNA gene amplicon sequencing demonstrated enrichment of sequences related to HOB (including bacteria from Mycobacterium, Hydrogenophaga, and Xanthobacter genera) over successive sub-cultures. Finally, four different HOB belonging to the Mycobacterium, Hydrogenophaga, Xanthobacter, and Acidovorax genera were isolated from reactor media, representing potential candidates as HBI system biocatalysts.

Heavy Metals Configuration in Different Saprobic Level of Water Bodies

The environmental conditions for aquaculture needs to be done in line with pollution that occur in waters which is categorized by saprobic index. The heavy metals content in cultured organisms present in the different levels of saprobity was informed by geospatial analysis through the overlay method. The Pb, Cd and Cr content were taken from tissues of milkfish, wild white shrimp and vaname shrimp. An Ikonos satelite Imagery data of Semarang used for geospatial analysis. The research was carried out from June to October 2021 in the brackish water ponds At Tugu district, Semarang, Central Java. The level of saprobity reported in oligosaprobic up to β-mesosaprobic which means lightly polluted to heavily polluted. The content in the water bodies (mg/L) of Pb 0.046 to 0.105, Cd 0.033 to 0.062 and Cr from 0.018 to 0.054 has exceeded the permittable level. Meanwhile, in the pond sediment (mg/kg) : Pb 3.56-6.93; Cr 1.08-3.01 is within permittable limits, however, Cd 1.77-3.53 has exceeded permittable levels. The content of heavy metals (mg/kg) in the tissues of milkfish, wild shrimp and white leg shrimp (L. vannamei): Pb 0.27 to 0.39, Cr 0.16 to 0.23 and Cd from 0.12 to 0.22 is still within permittable limits