Bovine Serum Antigens Research Articles

Effect of Sarang Banua (ClerodendrumfragransVent Willd) Leaves Extract on Serum Globulin Levels of Rabbit (Oryctolagus cuniculus)

Sarang banua plants are found in Simalungun, North Sumatra, Indonesia, which have been used by the community as traditional medicinal plants and is a type of ClerodendrumfragransVent Willd, including the family Verbanaceae. The study design was completely randomized design (CRD) with five treatments namely 1% Na-CMC (K1, negative control), bovine serum antigen (K2), ethanol extract of sarang banua leaf 200 mg/kg BW (K3), ethanol extract of sarang banua leaf 200 mg/kg BW with bovine serum antigen (K4) and ethanol extract of sarang banua leaf 150 mg/kg BW with bovine serum antigen (K5). The results showed that administration of ethanol extract of sarang banua leaf (ClerodendrumfragransVent Willd) significantly affected serum globulin levels of rabbits (Oryctolagus cuniculus). The ethanol extract of sarang banua leaves 200 mg/kg BW with bovine serum antigen (K4) produced the highest serum globulin levels of rabbits (12.91 mg/mL).

Isolation and Large Scale Expansion of Adult Human Endothelial Colony Forming Progenitor Cells

This paper introduces a novel recovery strategy for endothelial colony forming progenitor cells (ECFCs) from heparinized but otherwise unmanipulated adult human peripheral blood within a mean of 12 days. After large scale expansion >1x10(8) ECFCs can be obtained for further tests. Advantageously by using pHPL the contact of human cells with bovine serum antigens can be excluded. By flow cytometry and immunohistochemistry the isolated cells can be characterized as ECFC and their in vitro functionality to form vascular like structures can be tested in a matrigel assay. Further these cells can be subcutaneously injected in a mouse model to form functional, perfused vessels in vivo. After long term expansion and cryopreservation proliferation, function and genomic stability appear to be preserved. (3,4) This animal-protein free isolation and expansion method is easily applicable to generate a large quantity of ECFCs.

The effects of glaucoma filtering surgery on anterior chamber-associated immune deviation and contribution of lymphatic drainage in rats

To investigate whether glaucoma filtering surgery (GFS) in rats would impair the eye's capacity to induce anterior chamber-associated immune deviation (ACAID) and assess the possible mechanism involved. Rats subjected to GFS were injected with bovine serum antigen (BSA) into the anterior chamber to induce ACAID. Animals that had their cervical lymph nodes (CLNs) excised before filtering surgery and those that had sham filtering surgery served as control comparison groups. Antigen-specific delayed-type hypersensitivity (DTH) was used to identify the induction of ACAID. Antigen level in the CLNs was indicated by the percentage of FITC-positive cells in CLNs after FITC dextran was injected into the anterior chamber. Statistical analyses were performed using the student's t-test for comparison of data between control and experimental groups. A P<0.05 was required for results to be considered statistically significant. Rats undergoing GFS demonstrated antigen-specific DTH, while those in the sham filtering surgery or CLNs excised groups failed to acquire an antigen-specific DTH response. The percentage of FITC-positive cells in CLNs was significantly increased (P=0.001) in GFS (mean+/-SD: 2.96+/-0.67%) vs controls (1.57+/-0.48%) at 1 day, but not at 3, 5, 7, or 12 days post-antigen injection. GFS prevents the induction of ACAID in rats, and the antigen drainage to CLNs plays a critical role in this process. The results suggest that the ocular immune status might be altered by GFS.

Antigenicity of Proteins from Cultured Synovial Fibroblasts

We were able to characterize about 25 antigenic proteins of cultured synovial fibroblasts with apparent molecular weights of 15–230 kd by SDS-electrophoresis and immunoblotting using guinea pig antiserum. Some of these proteins were bovine-serum-derived, adsorbed onto fibroblasts from culture medium. Both rheumatoid and normal sera contained natural antibodies reacting with synovial fibroblast as well as bovine serum antigens when studied with immunoblotting. The amounts of antibodies were quantitated with enzyme immunoassay, measuring IgG and IgA class antibodies against plasma membrane preparations of rheumatoid and normal synovial fibroblasts. No rheumatoid arthritis-related changes were detected in synovial fibroblast protein antigens or antibodies against them in rheumatoid patient specimens.

Serotonin- and histamine-affecting agents and experimental vascular sensitization

1. 1. Nine serotonin- and histamine-affecting drugs were studied for an evaluation of their effects upon the development of lesions of hypersensitivity vasculitis produced in rabbits by passive reversed subanaphylactic sensitization techniques with bovine serum antigens. 2. 2. Only promethazine and cyproheptadine, with both potent antiserotonin and antihistamine actions, and the combination of chlorpromazine and tripelennamine were effective in suppressing and attenuating microscopic lesions of pulmonary periarteritis and panarteritis. 3. 3. Those pharmacologic agents whose actions include serotonin antagonism (brom-lysergic diethylamide, chlorpromazine, and hydroxyzine), serotonin release (reserpine), serotonin potentiation (iproniazid), and antihistaminics (tripelennamine and diphenhydramine) were relatively ineffective within the limits of this experimental procedure. 4. 4. It is suggested that the development of hypersensitivity vasculitis in the rabbit may be dependent upon multiple pathophysiologic processes, including those associated with tissue response to both serotonin and histamine release.