Bovine Fatty Acid Binding Protein Research Articles

Genetic variability of FABP4 c.328 G>A (rs110652478) polymorphism and its association with slaughter weight and carcass traits in Aberdeen Angus and Hereford bulls imported into Turkey

The study aimed to determine the genotypic distribution of the c.328 G>A (rs110652478) polymorphism in the bovine fatty acid-binding protein 4 (FABP4) gene and to determine its effect on slaughter weight in Aberdeen Angus and Hereford bulls imported into Turkey. A total of randomly selected 95 bulls were used in this study. Bulls were housed for fattening in semi-open pens for approximately nine months with the same management procedures. Genotyping of the FABP4 c.328 G>A (rs110652478) polymorphism, located in exon 3, was performed by the PCR-RFLP method. Allele and genotype frequencies were calculated by the standard procedures and the Hardy-Weinberg equilibrium was tested by comparing expected and observed genotype frequencies using chi-square and Fisher’s exact tests. Indices of genetic diversity were calculated based on the allelic frequencies. The least-squares method as applied in a GLM procedure was used to test the association between FABP4 c.328 G>A and phenotypic traits including slaughter weight, hot and cold carcass weight, chilling loss, dressing percentage, and carcass pH. Results revealed that the most frequent genotype was GG (49.18% and 52.94% for Angus and Herefords, respectively) and the minor allele frequency (A allele) was 0.32 in the total population. The genotype distribution was in agreement with Hardy Weinberg equilibrium (P>0.05). The moderately low levels of genetic variability observed in this study may be caused as a result of eventual inbreeding. PIC levels suggest that FABP4 c.328 G>A can be a mildly informative genetic marker for the tested population. Association analysis indicated that the effect of the FABP4 on slaughter weight was statistically significant (PA may provide valuable, but limited, information on imported beef cattle raised in Turkey conditions.

Association of Single Nucleotide Polymorphisms in the FABP4 Gene with Carcass Characteristics and Meat Quality in Holstein Bulls

Abstract The aim of this study was to characterize the bovine fatty acid binding protein (FABP4) gene 3691G>A and 2834C>G polymorphisms and to evaluate interaction effects on the live weight, carcass characteristics and meat quality of Holstein bulls in the South Marmara region of Turkey. A total of 400 Holstein bulls grown on a private farm and slaughtered at 14-21 months of age, were randomly selected for use in this study. Initially, genotyping was performed by PCR-RFLP method and statistical analysis was carried out using least square methods of the GLM procedure. A SNP located in intron 1 (2834C>G) was associated with desirable increases in live weight, hot carcass weight and chilled carcass weight, and a SNP located in exon 3 (3691G>A) was associated with significant increases in marbling scores and first compressive stress point of the longissimus dorsi muscle (P<0.05). The interaction analysis of the 3691G>A and the 2834C>G polymorphisms revealed significant effects for hot carcass weight, chilled carcass weight and backfat thickness (P<0.05). There were no significant associations between the SNPs and carcass measurements. Results indicated that the FABP4 3691G>A and 2834C>G polymorphisms and the 3691G>A and 2834C>G interactions can be used as selection parameters in breeding programmes to improve meat yield and carcass characteristics.

Tissue expression analysis, cloning, and characterization of the 5'-regulatory region of the bovine fatty acid binding protein 4 gene.

Fatty acid binding protein 4 (FABP4) is a member of the FABP family of proteins that bind fatty acids and play important roles in fatty acid uptake and intracellular transport. In the present study, we cloned the 5'-regulatory region of bovine and identified its transcription initiation sites. Sequence comparative analysis demonstrated amino acids and promoter sequences of were highly conservative in mammals. Real-time PCR analysis revealed the products of bovine were very highly expressed in subcutaneous adipose tissue. Serial deletion constructs of the bovine 5'-regulatory region evaluated in a dual-luciferase reporter assay showed that 209 bp upstream from the transcription initiation site was its core promoter. An electrophoretic mobility shift assay combined with a site-directed mutation experiment indicated that peroxisome proliferator activated receptor gamma (PPARγ), CCAAT/enhancer-binding protein β (C/EBPβ), and sterol regulatory element-binding protein (SREBP) were important transcription factors for bovine . These results provide an important basis for further understanding the regulation of bovine .

Association of Microsatellite Marker in FABP4 Gene with Marbling Score and Live Weight in Hanwoo

The bovine fatty acid binding protein 4 (FABP4) plays an important role to uptake intracellular fatty acid. It has been previously reported as a positional candidate gene for marbling score in livestock. The re-sequencing of FABP4 gene detected a polymorphic AT repeated sequence in intron II of FABP4 gene. Allelic distribution for this microsatellite marker was examined in other cattle breeds. A total of 8 alleles were detected with diverse repeat units (14 to 21 AT repeat) in Hanwoo and 7 breeds. Of the 8 alleles, the predominant alleles were (AT)16, (AT)18 and (AT)19 in the Hanwoo and 7 cattle breeds. The linear mixed model for genotypic effect (3237AT) on carcass traits showed a significant effect on marbling score (MAR P=0.025) and live weight (LWT; P=0.04) in the 583 Hanwoo cattle population. Live weight (LW) was highest in the homozygous (AT)17 genotype (557.5 ± 6.94) and lowest in the heterozygous (AT)16/17 genotype (521.7 ± 7.70). On the other hand, the homozygous (AT)17 genotype (3.0 ± 0.15) has the highest effect on marbling score and the lowest effect was in homozygous (AT)18 genotype (2.2 ± 0.15). The marbling score difference between both groups was 0.8 which is around two times higher than SNP genotype effect on marbling score in Limousin × Wagyu crosses. (Key words : Fatty acid binding protein 4 (FABP4), Microsatellite marker, Marbling score (MAR))

Spectroscopic investigations on the binding site of bovine hepatic fatty acid binding protein. Evidence for the existence of a single binding site for two fatty acid molecules

The hydrophobic region of the binding site of a bovine fatty acid binding protein (pI 7.0-FABP) has been characterized using fluorescence and circular dichroism (CD) spectroscopy. Blue-shifts of fluorescence emission maxima and increased lifetimes of naphthylamine dyes, anthroyloxy-fatty acids, pyrene nonanoic acid and trans-parinaric acid indicated a hydrophobic interaction with FABP. The fluorescence quenching of various anthroyloxy-fatty acids by iodide and acrylamide showed lower accessibility to the fluorophore linked to the carbon adjacent to the carbonyl group and towards the methyl end of the fatty acid. Binding stoichiometries were different for fatty acids and their bulky fluorescent analogues. trans-Parinaric acid when bound to FABP showed a complex induced CD-spectrum, which is explained by a close proximity of two ligands in the same binding site. Fluorescent derivatives of phosphatidylcholine with trans-parinaric acid and cholesteryl trans-parinarate did not bind to FABP. Thus, the binding site appears to be constructed for high affinity binding of long chain fatty acids.