Bovine Fasciolosis Research Articles

An application for mobile devices as a decision support for the treatment and prevention of bovine fasciolosis - A survey among organic dairy farms in Bavaria

Based on health applications used in human medicine for the self-management of chronic diseases, the aim of this study was to evaluate a mobile app in veterinary medicine using the example of a decision-making aid for the control and prevention of bovine fasciolosis on dairy cattle farms. The study was carried out on 37 organic dairy cattle farms in Bavaria. The farms were divided into two groups: one group received a mobile app (n=17) as a decision-making aid, while the other group received a printed brochure (n=20) with identical content. At the beginning of the study, all participants were questioned by telephone concerning their farm, among other things. After using the respective tool, the participants were again interviewed by telephone. At the beginning of the study, the majority of participants were aware of the options for drainage (n=28; 75.7%) and fencing off wet areas (n=36; 97.3%). 37.8% (n=14) stated that they were familiar with a pasture rotation system. After using the respective aid, the majority of participants described the tool as helpful (94.1% app; 80% brochure). Many participants (app group: n=10 (58.8%); brochure group: n=16 (80%)) stated that they wanted to change their approach based on the new knowledge gained, e. g. introducing a pasture rotation system (app group: n=9 (52.9%); brochure group: n=13 (65.0%)). Both study groups enjoyed working with the tool they used. They viewed the knowledge imparted by the mobile app or brochure as helpful and useful. Most participants expressed a desire to continue using these tools in animal health management on farms in the future. The use of tools such as mobile apps may improve veterinary advice, e. g. in the management of parasitoses in cattle herds.

Prevalence of bovine fasciolosis and direct financial losses in cattle slaughtered in Bamenda and Bafoussam abattoirs, Western Highlands, Cameroon.

Bovine fascioliasis is a parasitic disease that affects cattle. It leads to direct and indirect great economic loss due to animal mortalities, growth retardation and expenditure on anthelmintics, reduction livestock productivity and essentially condemnation of infected liver by inspection service. The study was carried out to determine the seasonal prevalence and estimated financial losses of fascioliasis in cattle in the Western Highlands of Cameroon. A total of 2167 cattle were selected. Later, the cattle, have being the carcasses, were dissected, and the livers were dissected and carefully examined for adult liver flukes. Faeces were collected immediately after the cattle were killed and examined using the formol-ether concentration technique. Condemned livers were weighted to estimate the financial losses using the average price of a kilogram of liver. A total 428 of cattle were found with infected livers or egg in the faeces giving a total prevalence of 19.75%. Among the infected carcass, 18.64% had both the Fasciola eggs in the faeces and flukes in the liver while 1.10% had only flukes in the liver. Results revealed that prevalence of Fasciola spp. was significantly higher during the rainy season (23.48%) than 16% in the dry season (p=0.00). A significantly (p=0.000) higher prevalence was also observed in females (33.9%) than in males (13.1%). Age range showed significant (p=0.000) influence with the animals of 8-10 years old recorded the highest prevalence of 39.3%. Furthermore, 433.1kg of liver was condemned giving a direct financial loss of 1221,550 FCFA (2049.64 USD) and an annual financial loss due to liver condemnation estimated to 1814,775 (3045.01 USD). Findings of this study indicated that bovine fascioliasis is prevalent in cattle in the Western Highlands of Cameroon, and it leads to an important financial loss particularly in the rainy season.

EPIDEMIOLOGY OF BOVINE FASCIOLIASIS: PREVALENCE AND HAEMATO-BIOCHEMICAL ALTERATIONS IN KATSINA ABATTOIR NORTH WESTERN NIGERIA

Bovine fascioliasis, caused by liver flukes Fasciola hepatica and Fasciola gigantica, significantly affects cattle health and production globally. This study evaluated the prevalence and haemato-biochemical changes associated with bovine fascioliasis in 134 cattle at Katsina Central Abattoir. Using the sedimentation technique, faecal samples revealed a 3.73% infection rate, with females showing a higher prevalence (4.55%) than males (2.17%), though not statistically significant (P > 0.05). The age group 2-3 years had the highest prevalence (25.00%), with significant differences among age groups (P < 0.05). Among breeds, Wadara had the highest prevalence (20.00%) compared to White Fulani (4.93%), with a significant difference (P < 0.05). Medium-conditioned cattle showed the highest prevalence (6.67%) versus good-conditioned ones (1.37%) with significant difference (P < 0.05). Infected cattle exhibited significant reductions (P < 0.05) in packed cell volume (PCV), haemoglobin (Hb), and total erythrocyte count (RBC), as well as decreases in white blood cell count (WBC), mean cellular volume (MCV), and mean cellular haemoglobin concentration (MCHC). Biochemical analysis indicated elevated alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), and total bilirubin (TB) levels (P < 0.05), with reduced albumin (ALB), total protein (TP), and conjugated bilirubin (CB) levels (P < 0.05). These results highlight the significant impact of bovine fascioliasis on cattle health in Katsina State, emphasizing the need for effective control strategies.

Prevalence of Bovine Fasciolosis with Fasciola Hepatica in the M'pila Slaughter Areas of Congo Brazzaville

Fasciolosis is a hepatobiliary dystomatosis due to the migration into the liver parenchyma of immature form of parasites called fasciola hepatica or liver fluke. It is a cosmopolitan disease which represents economic and health problems whose diagnosis is rarely made, it is usually a discovery at the slaughterhouse. It mainly affects animals and sometimes humans. It is in this context that this study was carried out at two slaughterhouses in Mpila in order to determine the prevalence of bovine fasciolosis according to breeds, age, sex, origin and slaughter sector. The liver incision technique to search for flukes was carried out on 356 cattle. The results of the analysis carried out on 356 cattle including 134 infested cattle showed a prevalence of 37.64%. Furthermore, the highest prevalence was observed among mixed race people, a rate of 53.84%. Thus, cattle aged over 4 years were the most exposed to this pathology. Our results show that fasciolosis is a reality in the slaughter areas of Brazzaville. Monitoring of this pathology must be carried out to protect the health of our animals and that of humans.

Estimation of diagnostic sensitivity and specificity of abattoir registrations and bulk tank milk ELISA as herd-level tests for Fasciola hepatica using Bayesian latent class modelling

The common liver fluke, Fasciola hepatica, is a trematode parasite found worldwide, typically with a focal distribution due to its requirement for suitable climatic and environmental conditions to complete its lifecycle. Bovine fasciolosis causes suboptimal production and economic losses, including liver condemnation at slaughter. The lack of reliable diagnostic methods is a disadvantage to the increasing demand for surveillance and control. The aim of this study was to evaluate the diagnostic accuracy of bulk tank milk (BTM) antibody testing and aggregated abattoir registrations (AAR) of liver fluke as herd-level tests for F. hepatica infection using Bayesian latent class models. Data from the abattoirs in 2019–2021 and BTM, sampled in the winter of 2020/2021, were collected from 437 herds on the southwest coast of Norway. The BTM samples were analysed with the SVANOVIR® F. hepatica-Ab ELISA test, with results given as an optical density ratio (ODR), and later dichotomized using the recommended cut-off value from the test manufacturer (ODR ≥0.3). Based on the BTM ELISA test, 47.8% of the herds tested positive. The AAR test was defined as the herd-level proportion of female slaughtered animals registered with liver fluke infection during the study period. For this test, three cut-offs were used (a proportion of 0.05, 0.1 and 0.2). The herds were split into two subpopulations (“Coastal” and “Inland”), which were expected to differ in true prevalence of F. hepatica infection based on climate-related and geographical factors. The diagnostic accuracies of both tests were estimated using Bayesian latent class models with minimally informative priors. Post-hoc analysis revealed that the maximum sum of sensitivity (Se) and specificity (Sp) of the tests was achieved with a herd-level proportion of ≥0.1 registered with liver fluke as the AAR test. Using this cut-off, the median estimate for the diagnostic accuracy of the BTM ELISA was 90.4% (84.0–96.2 95% Posterior Credible Interval (PCI)) for Se and 95.3% (90.6–100% PCI) for Sp, while the median estimate of Se for AAR was 87.5% (81.4–93.1% PCI) and the median estimate of Sp for AAR was 91.0% (85.2–96.5% PCI). The cut-off evaluation of the SVANOVIR® F. hepatica-Ab ELISA test for BTM confirmed the manufacturer's recommended cut-off of ODR ≥0.3 to denote positive and negative herds. This study suggests that AAR and BTM ELISA test can be used as herd-level tools to monitor liver fluke infection, so that appropriate interventions against infection can be implemented as necessary.

Diagnosis of fasciolosis antibodies in Brazilian cattle through ELISA employing both native and recombinant antigens.

The aim of this article was to identify antibodies against fasciolosis in cattle in Brazil. The methodology was reproduced in our laboratory and applied for the first time to the Brazilian cattle herd. The antigens tested can be used as a screening test and thus speed up the diagnosis of bovine fascioliasis.

Correlation between the Fasciola hepatica infection rate (number of parasites in the liver parenchyma) and the antibody titration, detected by ELISA assay.

This study aimed to demonstrate the existence of a possible correlation between the level of liver fluke infection in cattle caused by Fasciola hepatica and the titers of anti-parasite antibodies in the blood. 113 blood samples were taken from non-dewormed pasture cattle in the communal slaughterhouse of the Jijel city in order to determine the serological titration of anti-fluke antibodies by the ELISA method. After slaughtering the animals studied, a count of parasites present in the inspected livers was carried out in order to assess the level of parasitic infection. The prevalence of bovine fasciolosis (due to Fasciola hepatica) based on counting the number of flukes in the inspected livers was around 61.06%, while the seroprevalence obtained is 55.75%. The sensitivity and specificity of the ELISA test used in this study were, respectively, 91.3% and 100%. A very strong correlation (r = 0.93 to p < 0.0001) was statistically demonstrated between the antibody titers and the number of flukes in the inspected livers. According to the results of this study, there is a strong statistical correlation between serum antibody titers and the number of flukes present in the liver (the higher the number of parasites in the liver, the higher the level of anti-flukes antibodies in the blood).

Comparative Efficacy of Triclabendazole, Oxyclozanide and Nitroxynil against Bovine Fasciolosis in Gomal Zam Dam Command Areas of District Tank, Khyber Pakhtunkhwa

Comparative Efficacy of Triclabendazole, Oxyclozanide and Nitroxynil against Bovine Fasciolosis in Gomal Zam Dam Command Areas of District Tank, Khyber Pakhtunkhwa

Liver condemnation due to bovine and buffalo fasciolosis in Antioquia province, Colombia

Background: Fasciola hepatica affects cattle and buffaloes, causing decreased production and liver condemnation at the slaughterhouse level. Objective: To determine liver condemnation frequency, temporal distribution, and associated factors, as well as the spatial distribution and economic impact of F. hepatica infection, based on post mortem inspection in cattle and buffaloes processed between 2010 and 2019 in one of the main slaughterhouses in Colombia. Methods: An observational, descriptive, and cross37 sectional study was carried out, with a convenience non-probability sampling. The temporal distribution of the frequency of liver condemnations was analyzed, the Pearson ꭕ2 test was used to determine the association between the dependent variable (i.e. presence/absence of adult parasites of F. hepatica) and the frequency rates between months and years, and the monthly rainfall data was compared with the accumulated monthly frequency. The association between the dependent variable and the type of production (i.e. industrial-type bovine cattle—intended for milk production, commercial44 type bovine cattle —intended for meat production), weight, and sex (odds ratios, OR and confidence intervals, 95%CI) was explored. Descriptive maps of the municipalities that presented at least one positive case of F. hepatica in 2019 and of the weighted prevalences, both for bovines and buffaloes, were designed. Only losses due to liver condemnation between 2010 and 2019 were considered for the analysis of the economic impact. Results: A total of 1,947,233 animals were processed during the study period, with an overall frequency of fasciolosis of 3.1%. Industrial-type cattle presented a 7.4% higher risk of developing F. hepatica compared to commercial-type cattle (OR=0.074; 95%CI: 0.07-0.08). Likewise, male cattle presented 3.88 times more risk compared to females (OR=3.88; 95%CI: 3.49-4.33). Economic losses due to liver condemnation were estimated at US$1,129,660 for the study period. Conclusion: The frequency of condemnations in Colombia occurs throughout the year, although it increases in the rainy season. The economic losses, only estimated by liver condemnation due to fasciolosis, are important. However, it is necessary to explore such losses considering other productive indicators and in larger animal populations. Fasciola hepatica was reported for the first time in the country in buffaloes. This work demonstrates the importance of systematizing the information generated by animal slaughterhouses.

Random forest classification as a tool in epidemiological modelling: Identification of farm-specific characteristics relevant for the occurrence of Fasciola hepatica on German dairy farms.

Fasciola hepatica is an internal parasite of both human and veterinary relevance. In order to control fasciolosis, a multitude of attempts to predict the risk of infection such as risk maps or forecasting models have been developed. These attempts mainly focused on the influence of geo-climatic and meteorological features. Predicting bovine fasciolosis on farm level taking into account farm-specific settings yet remains challenging. In the present study, a new methodology for this purpose, a data-driven machine learning approach using a random forest classification algorithm was applied to a cross-sectional data set of farm characteristics, management regimes, and farmer aspects within two structurally different dairying regions in Germany in order to identify factors relevant for the occurrence of F. hepatica that could predict farm-level bulk tank milk positivity. The resulting models identified farm-specific key aspects in regard to the presence of F. hepatica. In study region North, farm-level production parameters (farm-level milk yield, farm-level milk fat, farm-level milk protein), leg hygiene, body condition (prevalence of overconditioned and underconditioned cows, respectively) and pasture access were identified as features relevant in regard to farm-level F. hepatica positivity. In study region South, pasture access together with farm-level lameness prevalence, farm-level prevalence of hock lesions, herd size, parity, and farm-level milk fat appeared to be important covariates. The stratification of the analysis by study region allows for the extrapolation of the results to similar settings of dairy husbandry. The local, region-specific modelling of F. hepatica presence in this work contributes to the understanding of on-farm aspects of F. hepatica appearance. The applied technique represents a novel approach in this context to model epidemiological data on fasciolosis which allows for the identification of farms at risk and together with additional findings in regard to the epidemiology of fasciolosis, can facilitate risk assessment and deepen our understanding of on-farm drivers of the occurrence of F. hepatica.

Prevalence and associated risk factors assessment of bovine fasciolosis in the Imbo Region, Burundi

Fasciolosis is a zoonosis that limits the productivity of ruminants worldwide, but there is a lack of information on its occurrence in Burundi. Therefore, this study aimed to fill the information gap by determining the prevalence and risk factors associated with bovine fasciolosis in the Imbo Region of Burundi. Two prevalence studies were conducted in parallel in the five communes of the five provinces in the Imbo region. In the first study, a total of 426 fecal samples were collected from randomly selected cattle farms and microscopically examined to determine Fasciola egg burden. Survey data on cattle husbandry were collected from owners of these cattle and analyzed to determine the risk factors for bovine fasciolosis. In the second study, 467 cattle were randomly selected in abattoirs and their livers were examined postmortem to determine liver fluke burdens. Data were entered separately into Microsoft Excel and analyzed using R software. The overall prevalence of bovine fasciolosis was 47.7% (42.9–52.4, 95% CI) for microscopic examination and 33.2% (28.9–37.5, 95% CI) for postmortem examinations. The majority of positive cattle (60.6%) had light intensity infections as determined by eggs per gram of feces (epg). Postmortem examinations corroborated these results and indicated that 80% of cattle had light intensity infections. Chi-square analysis showed a statistical association with the presence of bovine fasciolosis and the age, sex, and origin of cattle and the practices of cattle owners (P < 0.05).

Prevalence and Associated Risk Factors of Bovine Fasciolosis at Jima Rare Woreda, Ethiopia

A cross sectional study was conducted to determine prevalence and associated risk factors with fasciolosis in cattle at Jima Rare district, Ethiopia from June 2022 to November 2022. Examinations of the fasciolosis egg were performed by using sedimentation techniques. A total of 380 faecal samples from cattle were subjected to coprological investigation. Out of this 133 were positive for egg of Fasciola with an overall prevalence of 35% (133/380). The prevalence was 21.7% (20/92), 57.5% (54/94), 48% (46/96) and 13.3% (13/98) in Lenca Guracha, Dile kolba, Bebala and Misoma Dangago respectively. The variation of prevalence among kebeles were showed statistically significant difference (P&lt;0.05). The prevalence rates of fasciolosis based on sex were 35.5% (64/185) in females was higher when compared to 34.6% (69/195) in male sheep. However, the difference is not statistically significant (P&gt;0.05; X2 =0.03). Higher prevalence rate in adults 35.5% (65/181) was recorded when compared to young animals 34.2% (68/199). However, the difference is not statistically significant (P&gt;0.05; X2 =0.13). The prevalence rate among body condition is indicates that 43.2% (63/146), 34.8% (47/135) and 23.2% (23/99) in cattle with poor body condition, medium and good respectively. The analysis of data showed statistically significant (P&lt;0.05; X2 =10.3). As conclusion bovine fasciolosis was prevalent in district, thus causing major economic loss in the study area. Hence, control strategies targeted on the parasite and the intermediate hosts as well as implementation of appropriate grazing management in the study area were warranted.

Spatial analysis and risk mapping of Fasciola hepatica infection in dairy cattle at the Peruvian central highlands

This study aimed to develop maps for Fasciola hepatica infection occurrence in dairy cattle in the districts of Matahuasi and Baños in the Peruvian central highlands. For this, a model based on the correlation between environmental variables and the prevalence of infection was constructed. Flukefinder® coprological test were performed in samples from dairy cattle from 8 herds, during both the rainy and wet season. Grazing plots were geo-referenced to obtain information on environmental variables. Monthly temperature, monthly rainfall, elevation, slope, normalized difference vegetation index (NDVI), enhanced vegetation index (EVI), normalized difference water index (NDWI), distance to rivers, urban areas and roads were obtained by using remote sensor images and ArcGIS®. Multilayer perceptron Artificial Neural Networks modeling were applied to construct a predictive model for the occurrence of fasciolosis, based on the relationship between environmental variables and level of infection. Kappa coefficient (k > 0.6) was used to evaluate concordance between observed and forecasted risk by the model. Coprological results demonstrated an average prevalence from 20% to 100%, in Matahuasi, and between 0 and 87.5%, in Baños. A model with a high level of concordance between predicted and observed infection risk (k = 0.77) was obtained, having as major predicting variables: slope, NDWI, NDVI and EVI. Fasciolosis risk was categorized as low (p < 20%), medium (20% < p < 50%) and high (p ≥ 50%) level. Using ArcGIS 10.4.1, risk maps were developed for each risk level of fasciolosis. Maps of fasciolosis occurrence showed that 87.2% of Matahuasi area presented a high risk for bovine fasciolosis during the dry season, and 76.6% in the wet season. In contrast, 21.9% of Baños area had a high risk of infection during the dry season and 12.1% during the wet season. In conclusion, our model showed areas with high risk for fasciolosis occurrence in both districts during both dry and rainy periods. Slope, NDWI, NDVI and EVI were the major predictors for fasciolosis occurrence.

Bovine Fasciolosis in Slaughtered Cattle at Akinyele, Ibadan, Nigeria

Bovine Fasciolosis in Slaughtered Cattle at Akinyele, Ibadan, Nigeria

Prevalence of bovine fasciolosis in Jalingo abattoir, Taraba State, Nigeria

Prevalence of bovine fasciolosis in Jalingo abattoir, Taraba State, Nigeria

Green and Smart Quantitative Quality Control for Veterinary Mixture of Ivermectin and Clorsulon; Ecological Evaluation of Spectral Analyses via Analytical Eco-Scale, Green Analytical Procedure Index and Analytical GREEnness Metric Approaches.

Global financial market is still highly threatened by bovine fasciolosis; a parasitic infection, that targets cattle, mainly in tropical regions. Binary combination of Ivermectin (IVER) and Clorsulon (CLO), in challenging concentration ratios, is typically indicated for treatment and control of fasciolosis. Present study aims at smart simultaneous spectrophotometric assay of both compounds at their high ratio in marketed formulation and synthetic mixtures, without any prior separation. Furthermore, their greenness profile was evaluated and compared with previous reported assay methods including the official one. Mathematical-based proposed methods are Dual wavelength, Induced Dual wavelength and First Derivative Ratio methods. Each is developed, optimized and applied to determine simultaneously IVER and CLO at linear ranges of 1-30 and 5-40 μg/mL, respectively. They have been validated according to ICH guidelines. Statistical student t- and F-tests, compared the proposed methods with USP chromatographic technique. Ecological appraisal is accomplished using three independent metrics; Analytical Eco-Scale (AES), Green Analytical Procedure Index (GAPI) and Analytical GREEnness Metric Approach (AGREE). Satisfactory recoveries, ICH compliance and adherence of proposed methods to the ecological safety margin are achieved. Developed methods are eco-friendly, cost effective and can accomplish a routine quantitative quality control for concurrent determination of both drugs. Veterinary antimicrobials need analytical quality control using safer and green methodologies. Data manipulated spectral analyses of IVER and CLO, in ratio 1:10%v/v, is developed and optimized. AES, GAPI and AGREE approaches illustrate the high green compliance in respect to literary reported assays. Furthermore, the United States Pharmacopeia (USP) assay for IVER and CLO in injectable dosage form depends on analysis of each drug separately in the presence of the other drug but it cannot determine both drugs simultaneously at the same time.

Prevalence, Economic Loss, Risk Factor and Coprology of Bovine Fasciolosis in Selected District’s Municipal Abattoirs of Wolaita Zone, Southern Ethiopia

Prevalence, Economic Loss, Risk Factor and Coprology of Bovine Fasciolosis in Selected District’s Municipal Abattoirs of Wolaita Zone, Southern Ethiopia

Coprological and postmortem assessment and economic significance of bovine fasciolosis in cattle slaughtered at Tarcha Municipal Abattoir, Southern Ethiopia

Bovine fasciolosis caused by Fasciola hepatica and Fasciola gigantica, is a neglected tropical snail-borne trematode disease of cattle that poses an adverse effect on animals' health culminating in economic damage. Cross-sectional investigation on coprological and postmortem assessment and economic significance of bovine fasciolosis in cattle slaughtered at Tarcha Municipal Abattoir, Southern Ethiopia through simple random sampling techniques was conducted from December 2020 to August 2021. Out of 384 cattle examined, 20.3% and 29.94% were found harboring Fasciola through coprological examination and postmortem examination respectively. The leading cause of fasciolosis in the study area was Fasciola gigantica (53.9%) as compared to F. hepatica (46.1%). The investigation revealed that the sex, origin, and body condition of the cattle has a statistically significant effect on the prevalence of bovine fasciolosis. The odds of male animals infested with fasciolosis were 2.25 times higher than female cattle and those of poor body-conditioned cattle were three times higher than cattle with good body condition. In postmortem examination, an average of 6 flukes were found in a single infested liver and the mean fluke count was (10 ± 1.97) in severely infested livers while in lightly infested livers (3± 1.79). Considering postmortem examination as the gold standard for diagnosis of fasciolosis, the sensitivity of the direct sedimentation technique was found to be 68% and the specificity 100% with substantial agreement (k = 0.74) between the two methods. The estimated yearly direct financial losses incurred owing to fasciolosis was around 2,227,536 2, Birr (47,945.24 USD). Thus, bovine fasciolosis is one of the economically important animal diseases in the study area, which necessitates integrated control measures to tackle its effect on animal health and subsequent economic impact.

"Agro-Ecological Difference in Prevalence and Associated Risk Factor of Bovine Fasciolosis in Wolaita Zone, Ethiopia: Field Survey"

"Agro-Ecological Difference in Prevalence and Associated Risk Factor of Bovine Fasciolosis in Wolaita Zone, Ethiopia: Field Survey"

Influence of irrigation type in the prevalence of bovine fasciolosis, Cajamarca-Peru

Fasciolosis is a relevant zoonotic disease in the Peruvian Andes, it causes considerable economic losses by reducing cattle productivity and currently, resistance to anthelmintics is reported, so it is necessary to find new ways to comprehensively control this parasitosis. In this context, the objective of this study was to demonstrate the influence of the type of irrigation on the prevalence of bovine (Bos taurus) fasciolosis in dairy production units in the province of San Marcos, Cajamarca region, Peru. A total of 20 dairy cattle were introduced in two farms with different irrigation systems: traditional or flood irrigation (n = 10) and technified or sprinkler irrigation (n = 10). Starting in March and for ten consecutive months they were fed the rations produced on the farms and the prevalence of fasciolosis was monitored using the coproparasitological technique of natural sedimentation. In the livestock unit using flood irrigation, eggs excretion began in August, with a final prevalence of 60%; while in the livestock unit using sprinkler irrigation, the first cases were detected in September and the final prevalence was 20%. To the results, Fischer’s test determined that there was no association between the prevalence of the parasite and the type of irrigation (p=0.1698). The study suggests that the prevalence of fasciolosis in cattle fed rye-grass (Lolium perenne) / clover (Trifolium repens) grown with flood irrigation was higher than that of sprinkler irrigation; however, it is recommended to investigate with a larger number of samples and use more specific diagnostic methods.