AbstractThe spatial scale of submesoscales is an important parameter for studies of submesoscale dynamics and multiscale interactions. The horizontal spatial scales of baroclinic, geostrophic-branch mixed layer instabilities (MLI) are investigated globally (without the equatorial or Arctic oceans) based on observations and simulations in the surface and bottom mixed layers away from significant topography. Three high-vertical-resolution boundary layer schemes driven with profiles from a MITgcm global submesoscale-permitting model improve robustness. The fastest-growing MLI wavelength decreases toward the poles. The zonal median surface MLI wavelength is 51–2.9 km when estimated from the observations and from 32, 25, and 27 km to 2.5, 1.2, and 1.1 km under the K-profile parameterization (KPP), Mellor–Yamada (MY), and κ–ε schemes, respectively. The surface MLI wavelength has a strong seasonality with a median value 1.6 times smaller in summer (10 km) than winter (16 km) globally from the observations. The median bottom MLI wavelengths estimated from simulations are 2.1, 1.4, and 0.41 km globally under the KPP, MY, and κ–ε schemes, respectively, with little seasonality. The estimated required ocean model grid spacings to resolve wintertime surface mixed layer eddies are 1.9 km (50% of regions resolved) and 0.92 km (90%) globally. To resolve summertime eddies or MLI seasonality requires grids finer than 1.3 km (50%) and 0.55 km (90%). To resolve bottom mixed layer eddies, grids finer than 257, 178, and 51 m (50%) and 107, 87, and 17 m (90%) are estimated under the KPP, MY, and κ–ε schemes.