Population In Botswana Research Articles

Well-Being, Loneliness, and Quality of Relationships Before and During the COVID-19 Pandemic in Botswana

This paper explored the determinants of well-being, quality of relationships (QOR), and loneliness among people of Botswana before and during the novel COVID-19 pandemic. It also examined whether there were significant differences between participants’ well-being, loneliness, and QOR before and during the COVID-19 pandemic. Factors associated with the well-being, loneliness, and QOR of the Botswana population before and during the COVID-19 pandemic were also explored. A cross-sectional design was used to collect information on the three variables before and during the COVID-19 pandemic using online platforms. Convenience sampling was used to recruit participants ( N = 422) using the WHO-5, Three-Item Loneliness Scale, and the Relationship Quality Index (RQI). Data were analyzed using bivariate and multivariate mixed-effects linear models. Age, relationship status, concern about own and family health, difficulty switching on and off media reporting on COVID, change in lifestyle, participation in sports, and resting were significantly associated with well-being ( p < .001) and loneliness ( p < .05). Sleep was associated with QOR. There were significant differences in the well-being, QOR, and loneliness scores before COVID-19 conditions and during COVID-19 conditions. Stakeholders should take cognizance of the impact of predictor variables on well-being and loneliness to mitigate the impact of current and future pandemics.

Self-reported cardiovascular disease risk factor screening among people living with HIV vs. members of the general population in Botswana: a community-based study.

Morbidity and mortality due to cardiovascular diseases (CVDs) are high and increasing in low- and middle-income countries. People living with HIV (PLWH) are more likely to experience CVD than members of the general population. Therefore, we aimed to assess whether PLWH were more likely to have previously been screened for cardiovascular disease risk factors (CVDRFs) than people without HIV. A population-based, cross-sectional study was conducted among individuals aged 16 to 68 years across 22 communities in Botswana from February to August 2017 as part of a larger community-based cluster randomized HIV treatment-as-prevention trial. Participants were asked if they had been screened for and counselled on cardiovascular disease risk factors (history of hypertension or blood pressure check, blood glucose and cholesterol measurements, weight check and weight control, tobacco smoking and cessation, alcohol use and physical activity) in the preceding 3 years. HIV testing was offered to those with an unknown HIV status. Multiple logistic regression analysis controlling for age and sex was used to assess the relationship between CVDRF screening and HIV status. Of the 3981 participants enrolled, 2547 (64%) were female, and 1196 (30%) were PLWH (93% already on antiretroviral therapy [ART]). PLWH were more likely to report previous screening for diabetes (25% vs. 19%, p < 0.001), elevated cholesterol (17% vs. 12%, p < 0.001) and to have had their weight checked (76% vs. 55%, p < 0.001) than HIV-uninfected participants. PLWH were also more likely to have received counselling on salt intake (42% vs. 33%, p < 0.001), smoking cessation (66% vs. 46%, p < 0.001), weight control (38% vs. 29%, p < 0.001), physical activity (46% vs. 34%, p < 0.001) and alcohol consumption (35% vs. 23%, p < 0.001) than their HIV-uninfected counterparts. Overall, PLWH were more likely to have received screening for and/or counselling on CVDRFs (adjusted odds ratio 1.84, 95% CI: 1.46-2.32, p < 0.001). PLWH were almost two times more likely to have been previously screened for CVDRFs than those without HIV, indicating a need for universal scale-up of integrated management and prevention of CVDs in the HIV-uninfected population.

Whole genome sequencing reveals population diversity and variation in HIV-1 specific host genes.

HIV infection continues to be a major global public health issue. The population heterogeneity in susceptibility or resistance to HIV-1 and progression upon infection is attributable to, among other factors, host genetic variation. Therefore, identifying population-specific variation and genetic modifiers of HIV infectivity can catapult the invention of effective strategies against HIV-1 in African populations. Here, we investigated whole genome sequences of 390 unrelated HIV-positive and -negative individuals from Botswana. We report 27.7 million single nucleotide variations (SNVs) in the complete genomes of Botswana nationals, of which 2.8 million were missing in public databases. Our population structure analysis revealed a largely homogenous structure in the Botswana population. Admixture analysis showed elevated components shared between the Botswana population and the Niger-Congo (65.9%), Khoe-San (32.9%), and Europeans (1.1%) ancestries in the population of Botswana. Statistical significance of the mutational burden of deleterious and loss-of-function variants per gene against a null model was estimated. The most deleterious variants were enriched in five genes: ACTRT2 (the Actin Related Protein T2), HOXD12 (homeobox D12), ABCB5 (ATP binding cassette subfamily B member 5), ATP8B4 (ATPase phospholipid transporting 8B4) and ABCC12 (ATP Binding Cassette Subfamily C Member 12). These genes are enriched in the glycolysis and gluconeogenesis (p < 2.84e-6) pathways and therefore, may contribute to the emerging field of immunometabolism in which therapy against HIV-1 infection is being evaluated. Published transcriptomic evidence supports the role of the glycolysis/gluconeogenesis pathways in the regulation of susceptibility to HIV, and that cumulative effects of genetic modifiers in glycolysis/gluconeogenesis pathways may potentially have effects on the expression and clinical variability of HIV-1. Identified genes and pathways provide novel avenues for other interventions, with the potential for informing the design of new therapeutics.

Advancing Vocationalization of Open Schooling in Botswana: Addressing Critical Skills Gaps and Alleviating Unemployment

Vocationalization of open schooling is paramount for Botswana, a nation dedicated to sustainable development and economic growth. This article emphasizes the urgent need to integrate vocational education within the open schooling framework, focusing on its potential to address skills gaps, empower marginalized groups, and contribute to Botswana’s knowledge-based economy. By equipping the youth with relevant skills, vocationalized open schooling offers a pathway to alleviate future unemployment challenges. This article estimates the demographic potential benefiting from this approach, particularly the youth population of Botswana. Vocational education stands as a strategic imperative for Botswana, aligning with its goals of inclusive growth and equipping learners with practical skills that match industry demands. The convergence of vocational education and open schooling holds transformative power, providing accessible and relevant education to those historically excluded while preparing a capable and adaptable workforce. In a nation of approximately 2.35 million people, the youth represent both its strength and promise. Vocationalized open schooling emerges as a conduit to empower this demographic, ensuring they possess skills that align with industry needs and fostering a generation poised to contribute to the nation’s aspirations for progress and prosperity. As Botswana navigates the complexities of a changing global landscape, the integration of vocational education into open schooling shines as a beacon, illuminating pathways to employability, innovation, and socio-economic advancement.

Socio-economic differentials of under-five mortality in Botswana: an application of the indirect estimation method

BackgroundSocio-economic differentials in infant and under-five mortality exist in all countries worldwide, but the extent of differentials varies across countries. This study aimed to examine the socio-economic differentials of under-five mortality in Botswana within the framework of equality and equity.Methods and findingsThe analysis for this paper was based on the data from 2001 and 2011 Botswana Population and Housing Censuses and the 2017 Botswana Demographic Survey. One census method was used to indirectly estimate under-five mortality for different socio-economic groups using Princeton west model life tables. Results from this study revealed that socio-economic disparities in under-five mortality disfavouring the worse-off exist in all the selected socio-economic variables. The variations of socio-economic groups by mother’s place of residence and employment status have been narrowing over time, whereas the differences of socio-economic groups by mother’s level of education and marital status have been widening over time.ConclusionsThe findings of this study show that policies and interventions targeted at reducing inequalities should reach all socioeconomic groups across the country. Differences of each society and localities should be considered while designing and implementing policies and interventions.

No Difference in the Prevalence of HIV-1 gag Cytotoxic T-Lymphocyte-Associated Escape Mutations in Viral Sequences from Early and Late Parts of the HIV-1 Subtype C Pandemic in Botswana.

HIV is known to accumulate escape mutations in the gag gene in response to the immune response from cytotoxic T lymphocytes (CTLs). These mutations can occur within an individual as well as at a population level. The population of Botswana exhibits a high prevalence of HLA*B57 and HLA*B58, which are associated with effective immune control of HIV. In this retrospective cross-sectional investigation, HIV-1 gag gene sequences were analyzed from recently infected participants across two time periods which were 10 years apart: the early time point (ETP) and late time point (LTP). The prevalence of CTL escape mutations was relatively similar between the two time points-ETP (10.6%) and LTP (9.7%). The P17 protein had the most mutations (9.4%) out of the 36 mutations that were identified. Three mutations (A83T, K18R, Y79H) in P17 and T190A in P24 were unique to the ETP sequences at a prevalence of 2.4%, 4.9%, 7.3%, and 5%, respectively. Mutations unique to the LTP sequences were all in the P24 protein, including T190V (3%), E177D (6%), R264K (3%), G248D (1%), and M228L (11%). Mutation K331R was statistically higher in the ETP (10%) compared to the LTP (1%) sequences (p < 0.01), while H219Q was higher in the LTP (21%) compared to the ETP (5%) (p < 0.01). Phylogenetically, the gag sequences clustered dependently on the time points. We observed a slower adaptation of HIV-1C to CTL immune pressure at a population level in Botswana. These insights into the genetic diversity and sequence clustering of HIV-1C can aid in the design of future vaccine strategies.

Evaluation of the suitability of the Greulich and Pyle atlas in estimating age for the Botswana population using hand and wrist radiographs of young Botswana population

The applicability of Greulich and Pyle atlas for estimating age for court cases that require age estimation amongst the Botswana population is often questioned because the atlas has been adopted without being evaluated for Botswana. It has long been established that accuracies of such age estimation methods are dependent on different factors such as the general health and nutrition of the population. We present a study aimed at determining the level of accuracy of the Greulich and Pyle atlas at estimating age for the Botswana population. It is the first time that this type of study is being performed for Botswana. The results will be used to confirm if there is need to develop specific age estimation standards for Botswana. A total of 140 x-ray image samples were obtained from local hospitals for patients aged between 5 and 18 years. The sample images were compared with the Greulich and Pyle age estimation standards. In forensic age estimations, there are several important age limits which are considered. The legal age of maturity for Botswana is 18 years. This age limit is very important in determining whether offenders should be tried as children or adults and at the same time, in determining if victims are minors or adults. It also forms the basis for sentencing of offenders for crimes committed against children. For the important age group 15 ≤ 18 the mean skeletal age difference was found to be 0.94 (statistically significant) indicating that age was underestimated for males. This could be a problem where a perpetrator’s age is underestimated as a result not sentenced accordingly as they are mistaken for a minor. The mean skeletal age difference for females was found to be 0.64 years, indicating that age was underestimated by up to 0.64 years for females while the mean skeletal age difference was found to be 0.50 years for males in overall. This indicated that the Greulich and Pyle atlas underestimated age by 0.50 years overall. Significant statistical difference was observed between the mean chronological age and the mean estimated age as the p-values were found to be 0.03 for females and 0.05 for males. Based on these findings, it can be concluded that the Greulich and Pyle atlas can be used to estimate age but with caution as there is a possibility of errors. There is need for this atlas to be revised to reduce errors in age estimation for the Botswana context.

Prevalence and Associated Risk Factors of Anemia Among Hospitalized Children in a Tertiary Level Hospital in Botswana.

Anemia is a global health concern and has been associated with long term cognitive and behavioral adverse effects. A cross sectional study was conducted to determine the prevalence of and risk factors for anemia in infants and children between 6 months to 5 years of age admitted to a tertiary hospital in Botswana. Baseline full blood count of every patient admitted during the study period was assessed to determine if anemia was present. Data were collected from patient's medical inpatient chart, electronic medical record (Integrated Patient Management System (IPMS)), and through interviewing parents and caregivers. Multivariate logistic regression model was used to identify risk factors of anemia. A total of 250 patients were included in the study. Prevalence of anemia in this cohort was 42.8%. There were 145 (58%) males. Of the patients with anemia, 56.1%, 39.2%, and 4.7% had mild, moderate, and severe anemia, respectively. Microcytic anemia consistent with iron deficiency was identified in 61 (57%) patients. Age was the only independent predictor of anemia. Children aged 24 months and more had a 50% lower risk of having anemia than their younger counterparts (odds ratio (OR) 0.52; 95% Confidence Interval (95% CI) 0.30 to 0.89). The findings of this study demonstrate anemia as a serious health concern in the pediatric population in Botswana.

Non-seasonal Landsat based bare area gain detection in Botswana during 2002 to 2020 Period using Maximum Likelihood Classifier (MLC).

This paper estimates bare area gain detected using cloud free Landsat 7 (ETM+) and Landsat 8 (OLI) in Botswana. From 2002 to 2020, agricultural fields shrunk by 76.4%, while built-up increased by 49.2%, and bare areas increased from 3.32% to 7.03% (or 111.7%). There is a significant seasonal change in bare area detected reaching maximum during the dry season when there is little or no ground cover. In this study, the seasonality of bare area gain was overcome by only considering a bare area pixel to contribute to bare area gain if it exists during both the winter and summer months. The probability of bare area detection was 75.0% and probability of false detection 13.3% respectively. The 13% false detection tended to be built-up areas which had similar spectral characteristics as bare areas since most built-up areas have no ground cover. The bare area gain is driven by the high population growth rate of 3.4%. From 2001 to 2017, the population of the study area has increased by 34% and now accounts for 47% of the population of Botswana.

Acceptance rate and risk perception towards the COVID-19 vaccine in Botswana.

The COVID-19 disease burden continues to be high worldwide and vaccines continue to be developed to help combat the pandemic. Acceptance and risk perception for COVID-19 vaccines is unknown in Botswana despite the government's decision to roll out the vaccine nationally. This study aims to assess the acceptance rate and risk perception of COVID-19 vaccines amongst the general population in Botswana. We interviewed 5300 adults in Botswana from 1-28 February 2021 using self-administered questionnaires. The main outcomes of the study were vaccine acceptance and hesitancy rates. Demographic, experiential and socio-cultural factors were explored for their association with outcome variables. Two-thirds of the participants were females (3199), with those aged 24-54 making the highest proportion (61%). The acceptance rate of COVID-19 vaccine was 73.4% (95% CI: 72.2%-74.6%) with vaccine hesitancy at 31.3% (95% CI: 30.0%-32.6%). When the dependent variable was vaccine acceptance, males had higher odds of accepting the vaccine compared to females (OR = 1.2, 95% CI: 1.0, 1.4). Individuals aged 55-64 had high odds of accepting the vaccine compared to those aged 65 and above (OR = 1.2, 95% CI: 0.6, 2.5). The odds of accepting the vaccine for someone with primary school education were about 2.5 times that of an individual with post graduate level of education. Finally, individuals with comorbidities had higher odds (OR = 1.2, 95% CI: 1.0, 1.5) of accepting the vaccine compared to those without any underlying conditions. This study demonstrated a high acceptance rate for the COVID-19 vaccine and a low risk perception in Botswana. In order to achieve a high vaccine coverage and ensure a successful vaccination process, there is need to target populations with high vaccine hesitancy rates. A qualitative study to assess the factors associated with vaccine acceptance and hesitancy is recommended to provide an in-depth analysis of the findings.

Genetic diversity in L1 ORF of human papillomavirus in women with cervical cancer with and without human immunodeficiency virus in Botswana and Kenya

BackgroundThe variation of human papillomavirus (HPV) genotypes shapes the risks of cervical cancer and these variations are not well defined in Africa. Nucleotide changes within the L1 gene, nucleotide variability, and phylogeny were explored in relation to HIV in samples from Botswana and Kenya.MethodsA total of 98 HPV-positive cervical samples were sequenced to identify different HPV variants. Phylogenetic inferences were used to determine HPV genotypes and investigate the clustering of sequences between women living with HIV (WLWHIV) and -women not living with HIV (WNLWHIV).ResultsOut of 98 generated sequences, 83.7% (82/98) participants had high-risk (HR) HPV genotypes while 16.3% (16/98) had low-risk (LR) HPV genotypes. Among participants with HR-HPV genotypes, 47.6% (39/82) were coinfected with HIV. The prevalence of HR-HPV genotypes was statistically higher in the Botswana population compared to Kenya (p-value < 0.001). Multiple amino acid mutations were identified in both countries. Genetic diversity differed considerably among WLWHIV and WNLWHIV. The mean pairwise distances between HPV-16 between HIV and HIV/HPV as well as for HPV-18 were statistically significant. Six (6) new deleterious mutations were identified in the HPV genotypes based on the sequencing of the L1 region, HPV-16 (L441P, S343P), HPV-18 (S424P), HPV-45 (Q366H, Y365F), and HPV-84 (F458L). The majority of the patients with these mutations were co-infected with HIV.ConclusionsGenomic diversity and different genomic variants of HPV sequences were demonstrated. Candidate novel mutations within the L1 gene were identified in both countries which can be further investigated using functional assays.

The Role of Confucius Institute in Shaping China’s National Image in Botswana: Case of CI Students

Abstract The purpose of this article is to examine the role of Confucius Institute in Shaping China’s National Image in Botswana in the case of CI Students. The Confucius Institute at the University of Botswana (CIUB) is employed as a case study which is identified as the most active Chinese soft power instrument in Botswana. The paper uses a qualitative research method to help better understand the benefits and limitations of CIUB in creating China’s positive image in Botswana. Primary data is obtained from questionnaires whilst secondary data is derived from journal papers, thesis, and books. The study is framed by soft power theory to assist in learning how China utilises its soft power to improve its image in Botswana. The analysis revealed that CIUB has been fruitful in promoting Chinese language and culture in Botswana impacting positively on China’s national image. The findings of the study show that CIUB has the potential to clear some negative perceptions about China in Botswana. However, the limitation as indicated by the study is that CIUB does not have wide coverage of the population of Botswana as it targets mostly students. It is suggested that CIUB as a single tool cannot fully address all the concerns affecting China’s image.

The potential impact of country-level migration networks on HIV epidemics in sub-Saharan Africa: the case of Botswana.

Generalised HIV epidemics in sub-Saharan Africa show substantial geographical variation in prevalence, which is considered when designing epidemic control strategies. We hypothesise that the migratory behaviour of the general population of countries in sub-Saharan Africa could have a substantial effect on HIV epidemics and challenge the elimination effort. To test this hypothesis, we used census data from 2017 to identify, construct, and visualise the migration network of the population of Botswana, which has one of the most severe HIV epidemics worldwide. We found that, over 12 months, approximately 14% of the population moved their residency from one district to another. Four types of migration occurred: urban-to-urban, rural-to-urban, urban-to-rural, and rural-to-rural. Migration is leading to a marked geographical redistribution of the population, causing high rates of population turnover in some areas, and further concentrating the population in urban areas. The migration network could potentially be having a substantial effect on the HIV epidemic of Botswana: changing the location of high-transmission areas, generating cross-country transmission corridors, creating source-sink dynamics, and undermining control strategies. Large-scale migration networks could present a considerable challenge to eliminating HIV in Botswana and in other countries in sub-Saharan Africa, and should be considered when designing epidemic control strategies.

Recent Molecular Assessment of Plasmodium vivax and Plasmodium falciparum Asymptomatic Infections in Botswana.

.In 2016, we reported the presence of Plasmodium vivax in Botswana through active case detection. A real-time PCR was used during a similar study in 10 districts to assess changes in the P. vivax prevalence. We assessed 1,614 children (2–13 years of age) for hemoglobin (Hb; g/dL) and Plasmodium parasites. The median age of all participants was 5.0 years (25th percentile, 3 years; 75th percentile, 8 years). The median Hb (g/dL) level was 12.1, but 18.3% of the participants had anemia (Hb < 11.0 g/dL); these participants were clustered in the younger than 5 years age group in all districts (P < 0.001). The risk of anemia decreased with age 5 years or older (odds ratio [OR], 0.26; 95% confidence interval [CI], 0.197–0.34; P < 0.001). The prevalence rates of Plasmodium parasites were as follows: P. vivax, 12.7%; P. falciparum, 12.7%; P. malariae, 0.74%; and P. ovale (P. ovale curtisi), 0.68%. Mixed infection rates were as follows: P. falciparum and P. vivax, 2.35%; P. falciparum and P. ovale curtisi, 0.56%; P. vivax and P. malariae, 0.06%; and P. falciparum and P. malariae, 0.68%. The infections were largely asymptomatic (99.6%). Using logistic regression, the risk of infection with P. vivax was highest in Kweneng East (OR, 6.2; 95% CI, 2.9–13.1), followed by South East (OR, 5.6; 95% CI, 2.5–12.3) and Ngami (OR, 5.1; 95% CI, 2.2–12.0). Compared to the risk of infection for children younger than 5 years, the risk of infection decreased for children 5 years or older in regions with high rates of P. vivax and P. falciparum infections. P. vivax and P. falciparum have expanded within the asymptomatic population in Botswana; therefore, careful attention is required for their elimination.

Sociodemographic features of a cohort of people living with albinism in Botswana

BackgroundOculocutaneous albinism is disproportionately prevalent in Africa; however, the medical and psychosocial characteristics of people living with albinism (PWA) in Botswana have not been studied.ObjectiveTo characterize the demographics, health-related factors, sun-protective behaviors, and psychosocial challenges of PWA in Botswana.MethodsOverall, 50 PWA and 99 patients without albinism (non-PWA) were recruited and surveyed.ResultsHigher proportions of PWA lived in rural villages compared with non-PWA (odds ratio [OR], 2.59; 95% confidence interval [CI], 1.26-5.34). PWA reported limited access to health care more frequently compared with non-PWA (OR, 2.72; 95% CI, 1.11-6.62). High proportions of PWA adopted sun-protective measures, including sunscreen, clothing, and sunlight avoidance. Despite high rates of feeling accepted by family and peers, PWA had increased odds of feeling unaccepted by their community (OR, 15.16; 95% CI, 5.25-31.81), stigmatized by society (OR, 9.37; 95% CI, 3.43-35.62), and affected by stigma in social interactions (OR, 2.21; 95% CI, 1.08-4.54) compared with non-PWA. Three-quarters of PWA had witnessed mistreatment of PWA.LimitationsStudy limitations include the small sample size, convenience sampling, and a non-validated survey instrument.ConclusionPWA faced increased medical and psychosocial challenges compared with non-PWA in Botswana. Our findings can begin to inform public health strategies aimed at promoting improved health care, education, and social inclusion for this population in Botswana and other regions in Africa.

PRODUCTION AND MARKETING CONSTRAINTS OF SMALL STOCK FARMING: EVIDENCE FROM LIMID AND NON-LIMID FARMERS IN BOTETI SUB-DISTRICT, BOTSWANA

The majority of the rural population in Botswana keep small stock as a source of livelihood. However, small stock farmers face many constraints which impede maximization of their production and returns. Yet there is dearth of information on the major challenges they face. This study was intended to give an overview of major production and marketingconstraints faced by small stock farmers; and to identify factors influencing farmers’ participation in the LIMID program in Boteti sub-district, Botswana. Multistage samplingtechnique was used to collect data from 150 respondents selected randomly. Descriptive statistics, factor analysis and probit regression analytical techniques were used in data analysis. Factors that significantly influenced effective participation of small stock producers in the program are positive perception of the program, distance to a LIMID office, distance to a nearby cattle post, and household income. Production constraints included predators, theft, pasture scarcity, natural disasters, water scarcity and lack of transport. Further, marketing constraints were reported to be low prices, delayed payments from the government, poor roads and lack of marketing information. The study provides a basis for policy formulation to improve the effectiveness of smallholder farmers and develop measures required to help them improve their productivity.

Perceptions and attitudes towards acceptability of HIV self-testing among female sex workers in Selibe Phikwe, Botswana

Background: Sub-Saharan Africa accounts for 71% of the global burden of HIV infection. For the general population of Botswana, the estimated HIV prevalence is 18.5%; for female sex workers it is 61.9%. This study explored and documented female sex workers’ perceptions and attitudes towards the acceptability of HIV self-testing in Selibe Phikwe, north-eastern Botswana.Methods: Purposive convenience sampling and snowballing approaches were used to recruit 17 participants into the study which was carried out in collaboration with a community-based organisation, the Silence Kills Support Group. Two focus group discussions and five in-depth interviews were conducted. Information was collected on female sex workers’ awareness of HIV self-testing, their willingness to use it, their preferred distribution model, and their preferred test kit. Themes and subthemes that emerged were interpreted based on the Integrated Behavioural Model.Results: HIV self-testing (HIVST) was not known to most participants. Participants expressed negative attitude towards HIVST due to a lack of knowledge and confidence to carry out self-testing independently. Participants preferred facility-based services and a blood test over HIVST. Inadequate post-test counselling and lack of assisted HIVST were among their major concerns. Raising community awareness of HIVST through education was suggested.Conclusion: Improving the uptake of HIVST will require community sensitization, the availability of counselling services, close follow-ups, and the introduction of assisted HIVST approaches.

Combining molecular and incomplete observational data to inform management of southern white rhinoceros (Ceratotherium simum simum)

Conservation efforts have preserved the southern white rhinoceros (SWR) in protected areas and have resulted in substantial overall growth in population size, but in small, fragmented populations in which inbreeding is an important risk. However, field observation of breeding often lacks sufficient accuracy to inform translocation strategies that are intended to increase genetic variation. The purpose of this study was to integrate microsatellite genotypes with an incomplete, field-observed pedigree to make inferences about mean kinship and basic demographic data that could be used to inform translocation programmes for SWR in a confined population in Botswana. Using this approach, we identified parents for 29 out of 45 offspring born in the reserve between 1993 and 2013 and detected eight non-breeding bulls with high mean kinship as candidates for translocation. The method also allowed inferences about demographic parameters that could influence the effectiveness of intervention strategies, such as age and timing of reproduction, and natal sex ratios. Importantly, the reproductive dominance of the bulls was not as skewed as expected after the original dominant bull was removed from the population, suggesting that closed populations can maintain multiple, simultaneously breeding males. The genetic data also confirmed that the accuracy of field-based parentage assignment was increased after implementation of an ear-notching programme. This study demonstrates the value of combining genetic information with ongoing surveillance to inform management of threatened populations, and of using mean kinship to inform metapopulation management by identifying candidates for translocation.

Reaching marginalized young women for HIV prevention in Botswana: a pilot social network analysis.

Almost one-fifth of Botswana's population is infected with HIV. The Inter-Ministerial National Structural Intervention Trial is a trial to test the impact on HIV rates of a structural intervention that refocuses government structural support programs in favor of young women. Ensuring that the intervention reaches all vulnerable young women in any given community is a challenge. Door-to-door recruitment was inefficient in previous work, so we explored innovative ways to reach this population. We sought to understand the support networks of marginalized young women, and to test the possibility of using social networks to support universal recruitment in this population. Ego-centric and sociometric analyses were used to describe the support networks of marginalized young women. Marginalized young women go to other women and relatives for support, and they communicate face to face rather than using social media. Network maps show how young women were connected to each other. Lessons from the pilot include a better understanding of how to use social networks as a recruitment method, such as the time required and the types of community members that can help. Social networks could help reach other hard-to-reach populations.

Phylogeography and Population Structure of the Mediterranean Corn Borer, Sesamia nonagrioides (Lepidoptera: Noctuidae), Across Its Geographic Range.

Sesamia nonagrioides (Lefèbvre) (Lepidoptera: Noctuidae), is a widespread insect pest in Africa, the Middle East, and Europe. However, its pest status varies across its distribution range. It is a major pest of maize in Europe and of sugarcane in Iran. In Africa, it is a major pest of maize in West Africa but not considered as a pest in East Africa. Recent surveys conducted in 2015 recorded S. nonagrioides to be a major pest of sugarcane in Ethiopia and reported the species for the first time in Botswana, outside its known geographic range. The genetic relationship of these records with the previously recorded population of S. nonagrioides was investigated using the cytochrome oxidase subunit I region of the mitochondrial genome. In total, 113 individuals across the geographic range of the species were analyzed and 63 haplotypes were identified. Phylogenetic analysis separated the populations into two clades with no distinct geographic distribution pattern. The genetic differentiation was also not associated with host plants and geographic distances. Results of the molecular analysis revealed the long-time establishment of S. nonagrioides population in Botswana and identified the newly recorded sugarcane population from Ethiopia as part of the wild host population in the country. The phylogeographic patterns observed among population of S. nonagrioides have probably been shaped by Pleistocene's climatic oscillations and geographic range expansions from different refugia with secondary contact and admixture. Possible reasons for the host-plant expansion by the Ethiopian population are discussed.