Boric Acid Concentration Research Articles

Boric acid supplementation promotes the development of in vitro-produced mouse embryos by related pluripotent and antioxidant genes.

Boric acid (BA) is an important mineral for plants, animals and humans that assists metabolic function and has both positive and negative effects on biological systems. The present study aimed to investigate the effects of different concentrations of BA added to the culture media, the quality and in vitro development potential of mouse embryos. Superovulated C57Bl6/6j female mice were sacrificed ∼18 hours after human chorionic gonadotropin (hCG) injection. Single-cell-stage embryos were collected from the oviduct, divided into experiment groups and cultured in embryo medium with supplemented BA+ in 5% CO2 at 37 °C until 96 hours at the blastocyst stage. The blastocyst development rates of 0, 1.62 × 10-1, 1.62 × 10-2, 1.62 × 10-3 and 1.62 × 10-4 µM BA were 51.52%, 73.47%, 77.36% and 81.13%, respectively. The in vitro development rates were significantly higher in the 1.62 × 10-3 (p < 0.05) and 1.62 × 10-4 µM BA groups than in the control group (p < 0.001). These results indicated that low BA doses influenced embryo development by positively affecting in vitro development rates, embryo cell numbers, biochemical parameters and development at the molecular level by pluripotent and antioxidant genes. Therefore, BA seems to play an important role on in vitro embryo development.

Lethal disruption of the bacterial gut community in Eastern subterranean termite caused by boric acid.

The Eastern subterranean termite, Reticulitermes flavipes (Kollar) (Blattodea: Rhinotermitidae), is a significant pest, causing extensive damage to structures that amount to substantial economic losses. Boric acid is widely used for wood preservation due to its stability and broad-spectrum insecticidal properties, yet its impact on termite gut microbiomes and the implications of such effects remain understudied. Our study evaluates the dose-dependent mortality of R. flavipes upon being provided boric acid treated filter papers and investigates the resulting dysbiosis within the termite gut microbiome. Consistent with reports from other insects, mortality increased in a dose-dependent manner, with the highest boric acid concentration (203.7 µg/cm2 of filter paper) significantly reducing termite survival. 16S rRNA gene sequencing of the gut bacterial microbiome revealed notable shifts in composition, indicating boric acid-induced dysbiosis. Aside from an overall decrease in bacterial diversity, the relative abundance of some symbionts essential for termite nutrition decreased in response to higher boric acid concentrations, while several opportunistic pathogens increased. Our findings extend the understanding of boric acid's mode of action in termites, emphasizing its ability to significantly modulate the bacterial symbiont community, which can have dire effects on termite biology. Considering its ability to protect wood from further termite consumption, our study supports the continued use of boric acid and related compounds for termite-resistant treatments for wood.

Improving fruit set and quality of Daisy mandarin through foliar application of nutrients

Daisy mandarin is a widely cultivated citrus species valued for its nutritional qualities and organoleptic properties, favored for both fresh consumption and processing. Optimal growth and fruit production is depend upon the availability of essential nutrients. However, soils of the central zones of Punjab are deficient in potassium which hinders the quality and production and their deficiency impairs physiological and biochemical processes, reducing fruit set, yield and quality. Potassium and calcium are vital for metabolic functions and cell wall integrity. A deficiency in any of these nutrients can results in smaller fruits, lower fruit retention and diminished organoleptic and nutritional qualities, such as reduced antioxidants content. So that, a research was conducted to evaluate the effect of different concentrations of potassium nitrate, potassium sulfate, calcium nitrate and boric acid along with paddy straw mulching on Daisy mandarin during the years 2021–22. The pooled data of this research showed that potassium nitrate (2%), is the best treatment with maximum fruit set (14.37%) and retention (72.35%) in Daisy mandarin. Additionally, the average fruit weight (207.50 g), number of fruits (162), fruit yield (33.79 kg/tree), fruit length (6.25 cm) and breadth (6.73 cm) also enhanced. Furthermore, potassium nitrate (2%) application increased the antioxidant activity (39.43%), phenolic content (230.00 mg kg−1 FW) and flavonoid content (3.69 mg RE g−1 FW). The findings suggest that application of potassium nitrate presents a promising strategy for enhancing fruit set, yield and quality of Daisy mandarin, providing valuable insights for improved cultivation practices.

Influence of boric acid on the speciation of iron (III) in aqueous solutions

During the work, the speciation of iron (III) was studied at the concentration of ~ 1∙10–5 mol/dm3 in aqueous solutions in the presence of boric acid at a concentration of 5–20 g/dm3 in a wide pH range using ion exchange, ultrafiltration and centrifugation methods. The regions of existence of ionic and nonionic forms of iron (III) were determined by ion exchange in solutions with different boric acid concentrations. Experimental data have shown that the pH of the onset of iron (III) colloid formation shifts in the presence of boric acid. The formation of complex compounds of iron (III) with polyborate ions in the neutral – slightly alkaline pH region was established by ultrafiltration and centrifugation. As shown in the paper, the increase in the concentration of boric acid in solution leads to the increase in the region of existence of the supposed complexes. The identified features of the behavior of iron (III) in solutions containing boric acid determine the possibility of using ion exchange and membrane separation methods for purifying solutions. Information about the physical-chemical properties of various metal-ions can provide optimal conditions for the purification of liquid radioactive waste at various nuclear industry facilities.

Optimizations of Liquid Phase Deposition Processes for Enhanced Photoelectrocatalytic Activities of Tungsten Oxide Thin Films.

This study focuses on the preparation of tungsten oxide (WO3) as the photoanode for water oxidations by the liquid phase deposition (LPD) technique and its optimizations to improve the photoelectrochemical performance. The alternative precursor large stock solution process was achieved to simplify the LPD process for WO3 thin film preparation. The effect of boric acid in the precursor solutions on the physicochemical properties of the deposited WO3 thin films was investigated. As a result, we found that the optimized concentration of boric acid realized the highest photoelectrochemical performance. Through the optimizations of reaction conditions and surface analyses, we concluded that the preparations of a semiconductor film via the LPD technique had the potential to obtain high-performance photoelectrocatalytic applications.

Characteristics of heat transfer in corrosion products deposited on the surface of pressurized water reactor fuel elements

The occurrence of porous deposits on the core surface during the operation of a pressurized water reactor can seriously affect its heat transfer characteristics and increase the surface temperature of the cladding. Continued heat transfer deterioration causes subcooled boiling and boron enrichment within porous deposits, leading to axial power anomalies in the core, and the study of porous deposits is beneficial to improving the economics and safety of reactor operation. In this paper, a two-dimensional boiling unit model of a porous deposits was established using COMSOL software to couple heat conduction, darcy flow, solute transport and chemical reactions in a multiphysics field to reasonably predict the temperature field, velocity field, boric acid concentration distribution, and PH distribution within the porous deposits. On this basis, the influence of different parameters (porosity, boiling unit radius of porous deposits, heat flow density) on the maximum effective thermal conductivity, the pattern of thermal parameters (temperature, superheat, percentage of subcooled boiling, and saturation temperature) on the chimney boundary are analyzed. The results show that localized superheating, maximum boric acid concentration and subcooled boiling occur at the bottom of the porous deposits. There is a negative correlation between the porosity and the percentage of subcooled boiling area, and a positive correlation between the radius of the porous deposits and the heat flow density and the percentage of subcooled boiling area.

Influence of nicotinic acid additive on the electrodeposition of nickel from aqueous solution

In nickel-plating aqueous baths, both organic and inorganic additives are employed to enhance surface finish, reduce roughness, and achieve a uniform morphology of the coatings. This study investigates how four different concentrations of nicotinic acid (NA) additive, combined with a fixed concentration of boric acid, influence nickel electrodeposition in an aqueous solution, with minimal effect on the speciation of Ni(II) clusters. NA inhibits the Ni(II) reduction process even at a low concentration of 0.01 M, resulting in negligible changes in behavior compared to the additive-free environment. A higher concentration of 0.06 M NA is required to achieve a high-quality surface. The study demonstrates that the inclusion of NA significantly alters the electrochemical behavior relative to the additive-free regime. For instance, the use of both additive systems leads to coatings with relatively high microhardness and brighter, smoother deposits.

Determining appropriate methods for estimating pollen viability and germination rates in lisianthus

Crossbreeding is a multi-stage process with inherent challenges and risks in developing new varieties. Success hinges on selecting highly fertile parents. In species like lisianthus, uncertainty persists regarding the optimal methods for assessing pollen quality, which is crucial for evaluating pollen parent fertility. This study seeks to identify the most reliable techniques for this purpose. Fresh and dead pollen from four lisianthus (Eustoma grandiflorum) varieties was used. The dead pollen was obtained by thermal inactivation. Five chemical staining methods (iodine-potassium iodide, 2,3,5-triphenyltetrazolium chloride – TTC, lactophenol cotton blue, safranin, acetocarmine) were employed to assess pollen viability, and two biological methods (Petri dishes, hanging drops) were used to determine the germination rate. Four solid medium cultures were employed in Petri dishes, while the hanging drop utilised four liquid medium cultures. Thirteen tests were conducted for each variety, evaluating fresh and dead pollen. The study found significant variations in pollen quality among lisianthus varieties and methods. Fresh pollen showed viability rates ranging from 56.87% to 99.41% and germination rates from 0.20% to 45.11%. TTC exhibited the lowest viability rate across all varieties, while the highest germination rate was observed in the liquid culture medium with only boric acid and PEG1500. Notably, TTC was the sole viability method that did not stain dead pollen, and no germination occurred in any method for dead pollen. TTC is the most reliable staining method, and a liquid culture medium with boric acid and PEG1500 effectively determines lisianthus pollen quality. Varying boric acid and PEG1500 concentrations are advisable.

Research on fuzzy adaptive GPC of average coolant temperature based on regulation of boric acid concentration

In order to keep the balance of power distribution in the core of a nuclear reactor, the method of adjusting the boric acid concentration to regulate the average temperature of the coolant has been adopted to track the demands of power changes in the core. However, the chemical reaction process of regulating the average coolant temperature by adjusting the boric acid concentration is too slow to better meet the demands of power changes. In view of this challenge, this paper proposes an innovative fuzzy adaptive incremental generalized predictive control strategy to optimize the process of regulating the coolant temperature. This strategy employs a dynamic adjustment of control parameters, specifically through the application of incremental factors γ, which is calibrated using a fuzzy logic-based algorithm to enhance system responsiveness and robustness in real-time. The strategy aims to improve the efficiency and responsiveness of the nuclear reactor control mechanism and further enhance the safety and stability of nuclear power generation.Firstly, the system state space equations are established based on the boric acid concentration equation and the nuclear reactor kinetics. Secondly, the step factors β and incremental factors γ are used in improved-GPC to improve control real-time and robustness. The step factor β speeds up the rolling optimization, and the incremental factor γ is adjusted based on the fuzzy algorithm according to the online recognition degree of the system. Finally, the 1000 MW Pressurized Water Reactor is modeled and simulated. The results show that improved-GPC has a shorter regulation time and a stronger ability to cope with the model mismatch in this system than other controllers.

Evaluation of Possible Toxic Effects of Boric Acid in Palourde Clam (Ruditapes decussatus) Through Histological Changes and Oxidative Responses.

The extensive utilization of boric acid, particularly in industrial and agricultural sectors, also engenders concerns regarding the toxicity of boron and its derivatives. Particularly, the behavior of boric acid at increasing concentrations in aquatic ecosystems remains poorly understood. In light of these concerns, this study aimed to investigate the toxicity of boric acid in bivalves, which occupy a critical position in the food chain. Specimens of Ruditapes decussatus, which had not been previously exposed to any pollutants and were cultivated under controlled conditions, were subjected to three different concentrations of boric acid (0.05mg/L, 0.5mg/L, and 5mg/L) in vitro for 96h. Following the exposure period, the specimens were assessed for histological changes (the mantle, gill, and digestive gland) and specific oxidative parameters (the gill and digestive gland), including superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase, and lipid peroxidation (LPO). The research findings indicated that boric acid primarily induced oxidative damage at the applied concentrations and increased antioxidant levels (p < 0.05). Moreover, although no significant histopathological abnormalities were observed in the examined histological sections, subtle changes were noted. This study evaluated the potential adverse effects of boric acid on bivalves, which are crucial components of the aquatic food chain, utilizing histological and specific physiological parameters following its introduction into aquatic environments. It is anticipated that the findings of this study will contribute to the development of new insights and perspectives regarding the extensive use of boric acid.

Concentration-Dependent Effects of Boric Acid on Osteogenic Differentiation of Vascular Smooth Muscle Cells.

Vascular calcification can be triggered by oxidative stress and inflammation. Although boron possesses antioxidant and anti-inflammatory properties, its effect on osteogenic differentiation of vascular smooth muscle cells (VSMCs) has yet to be examined. Therefore, we aimed to investigate the effect of boric acid (BA), the main form of boron in body fluids, on the osteogenic differentiation of VSMCs. Following the isolation of VSMCs, the effects of BA on cell proliferation were determined by MTT. The impact of various BA concentrations on the osteogenic differentiation of VSMCs was evaluated by Alizarin red S and alkaline phosphatase (ALP) stainings and the o-cresolphthalein complexone method. In addition, mRNA expressions of osteogenic-related (Runx2 and ALP) and antioxidant system-related genes (Nrf2 and Nqo1) were detected using qRT-PCR analysis. BA treatments did not alter the proliferation of VSMCs. Osteogenic differentiation of VSMCs treated with 100 and 500μM BA (moderate and high plasma concentrations) was no different from untreated cells. However, increased osteogenic differentiation was observed with the lowest blood level (2μM) and extremely high BA concentration (1000μM). Consistent with these results, mRNA expression of Runx2 increased with 2 and 1000μM BA treatments, while Nrf2 and Nqo1 expressions increased significantly with 100 and 500μM BA. BA has different effects on VSMCs at various concentrations. The low blood level and too high BA concentration appear detrimental as they increase the osteogenic differentiation of VSMCs in vitro. We propose to investigate BA's effects and mechanism of action on vascular calcification in vivo.

In Vitro Effects of Boric Acid on Cell Cycle, Apoptosis, and miRNAs in Medullary Thyroid Cancer Cells.

Medullary thyroid cancer (MTC) is a highly aggressive and chemotherapy-resistant cancer originating from the thyroid's parafollicular C cells. Due to its resistance to conventional treatments, alternative therapies such as boric acid have been explored. Boric acid, a boron-based compound, has shown anticarcinogenic effects, positioning it as a potential treatment option for MTC. TT medullary thyroid carcinoma cell line (TT cells) and human thyroid fibroblast (HThF cells) were utilized for the cell culture experiments. Cell viability was assessed using the 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay. Total RNA was extracted using Trizol reagent for gene expression and microRNA (miRNA) analysis via reverse transcription-polymerase chain reaction (RT-PCR). The extent of apoptosis induced by boric acid was determined using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Colony formation assays were conducted to evaluate the impact of boric acid on the colony-forming ability of MTC cells. At 48h, 50% inhibitory concentration (IC50) of boric acid was found to be 35μM. Treatment with boric acid resulted in significant modulation of apoptosis-related genes and miRNAs, including increased expression of phorbol-12-myristate-13-acetate-induced protein 1(NOXA), apoptotic protease activating factor 1 (APAF-1), Bcl-2-associated X protein (Bax), caspase-3, and caspase-9. In contrast, the expression of B cell lymphoma 2 (Bcl2), B cell lymphoma- extra-large (Bcl-xl), and microRNA-21 (miR-21), which are linked to the aggressiveness of MTC, was significantly reduced. The TUNEL assay indicated a 14% apoptosis rate, and there was a 67.9% reduction in colony formation, as shown by the colony formation assay. Our study suggests that boric acid may have anticancer activity in MTC by modulating apoptotic pathways. These findings suggest that boric acid could be a potential therapeutic agent for MTC and possibly for other malignancies with similar pathogenic mechanisms.

Investigation of VVER-1000 fuel assembly bowing effect on power distribution during cycle using neutron noise adiabatic approximation

Thermomechanical and radiation loads in the reactor core cause lateral changes in the shape of Fuel Assemblies (FAs). This phenomenon, known as FA bowing, has significant implications for reactor safety and operation. Bowing results in alterations to the spacing between FAs, which in turn leads to neutron flux perturbations affecting power distribution. These perturbations are time-dependent due to variations in boric acid concentration during the cycle. Neutron noise theory is employed to analyze these time-dependent neutron perturbations. In this research, it is investigated the bowing effect during the cycle in a VVER-1000 reactor using the neutron noise adiabatic approximation. It was initially considered a VVER-1000 reactor core to verify the calculations algorithm without bowed FAs. Results had acceptable accuracy in power, initial boric acid, and cycle length. Subsequently, it was explored various modes of FA bowing as neutron noise sources the results revealed that the power noise caused by bowing varies throughout the cycle, as expected. The noise amplitude during the cycle depends on changes in boric acid concentration, burnup, size, and the direction of bowing.

A comprehensive analysis of thermal–hydraulic signatures in neutron noise of WWER-type reactors

The neutron noise phenomenon, occurring in all types of nuclear reactors, provides valuable insights into the dynamic behavior of these reactors. In this work, a well-justified approach for modeling neutron noise induced by thermal–hydraulic sources in a hexagonal reactor core is presented in detail. These sources encompass fluctuations in key features of the inlet coolant, including flow rate, temperature, and boric acid concentration. Our proposed approach touches on various aspects of the distribution of thermal–hydraulic parameters among coolant loops, accounting for both uniform and non-uniform distributions. The latter allows for the asymmetric and asynchronous distribution of their fluctuating component among the coolant loops. To manage such complexity, it becomes imperative to determine the contribution of each fuel assembly based on each coolant loop. To achieve this, CFD simulations are conducted for the downcomer and lower plenum of the reactor pressure vessel. These simulations provide essential data to establish the coolant mixing matrix, which accounts for non-uniform distributions. The obtained mixing matrix is subsequently integrated into our neutron noise simulator, DYNOSIM, ensuring the faithful reproduction of neutron noise. Our study comprehensively analyzes both uniform and non-uniform scenarios. The radial and axial distributions of neutron noise amplitude and phase are examined for these scenarios. Their qualitative verifications are conducted by prior works in this field. Furthermore, we compare one of the uniform scenarios with reference results obtained using a frequency domain simulator. Notably, our CFD simulation results closely match the experimental data. Our findings demonstrate the effectiveness and efficiency of this methodology in modeling and understanding neutron noise in hexagonal reactor cores.

Genome-Wide Screens Identify Genes Responsible for Intrinsic Boric Acid Resistance in Escherichia coli.

Boric acid (BA) has antimicrobial properties and is used to combat bacterial infections, including Enterobacteria. However, the molecular mechanisms and cellular responses to BA are still unknown. This genomics study aims to provide new information on the genes and molecular mechanisms related to the antimicrobial effect of BA in Escherichia coli. The Keio collection of E. coli was used to screen 3985 single-gene knockout strains in order to identify mutant strains that were sensitive or hypersensitive to BA at certain concentrations. The mutant strains were exposed to different concentrations of BA ranging from 0 to 120mM in LB media. Through genome-wide screens, 92 mutants were identified that were relatively sensitive to BA at least at one concentration tested. The related biological processes in the particular cellular system were listed. This study demonstrates that intrinsic BA resistance is the result of various mechanisms acting together. Additionally, we identified eighteen out of ninety-two mutant strains (Delta_aceF, aroK, cheZ, dinJ, galS, garP, glxK, nohA, talB, torR, trmU, trpR, yddE, yfeS, ygaV, ylaC, yoaC, yohN) that exhibited sensitivity using other methods. To increase sensitivity to BA, we constructed double and triple knockout mutants of the selected sensitive mutants. In certain instances, engineered double and triple mutants exhibited significantly amplified effects. Overall, our analysis of these findings offers further understanding of the mechanisms behind BA toxicity and intrinsic resistance in E. coli.

Enhancement of mechanical behaviors of the 3D-printed polyvinyl alcohol–based scaffold by boric acid crosslinking

Abstract Polyvinyl alcohol (PVA)-based scaffold fabricated by fused deposition modeling (FDM) shows great potential in cartilage repair field. However, the limited mechanical properties after being swollen by water molecules in the body fluid hinder their applications. Herein, the boric acid is introduced to improve the mechanical behaviors of FDM-printed PVA-based scaffold. The ICP, FTIR, SEM, and swelling behaviors are utilized to explore the influence of boric acid concentration on the materials. The results indicate that the boric acid would form boronic ester-crosslinked PVA (B-PVA) and the density of the crosslink will increase at first then decrease with the boric acid concentration increases. As the concentration of boric acid is 1 %, the densest crosslink point in materials can be obtained. Then the fatigue, relaxation, and creep behaviors tests are carried out, which indicates that the crosslinking will improve the mechanical behaviors of scaffold at a great level. At last, the scaffold shows a good mineralization ability and excellent biocompatibility.

Boric Acid Affects the Expression of DNA Double-Strand Break Repair Factors in A549 Cells and A549 Cancer Stem Cells: An In Vitro Study

DNA double-strand break (DSB) repair genes interact with tumor stemness- and resistance-associated processes in cancer stem cells (CSCs). Therefore, targeting DNA DSB genes in cancer treatment is important for the CSC phenotype. Although the anti-cancer effect of boric acid (BA) has been studied, its effect on DNA DSB is unclear. Moreover, no studies investigate BA’s effects on DNA DSB of lung cancer stem cells (LC-SCs). To fill the gap, we aimed to assess the effects of BA on A549 cancer stem cells. CSCs were isolated from human non-small cell lung cancer cells (A549) and characterized by flow cytometry. Different concentrations of BA (at doses ranging from 1 to 100 mM) were applied to cancer stem cells. Cytotoxic activities were determined using the cell viability assay (MTT assay) at 24 and 48 h. Expression levels of DNA DSB genes that BRCA1, BRCA2, RAD51, KU70/80, ATM, and XRCC4 were evaluated by RT-qPCR. Additionally, immunofluorescence staining analysis was exploited for caspase-3 and E-cadherin. ATM expression increased significantly (p < 0.001). No significant change was observed in the expression of other genes. Moreover, BA up-regulated caspase-3 and E-cadherin expression. Consequently, we can say that BA affects DNA DSB and the apoptotic abilities of LC-SCs.Graphical

Interaction effect of boron and zinc on growth, yield and quality of guava (Psidium gaujava L.) Cv. ‘Lalit’

The present investigation was carried out at Agricultural Research Farm, Suresh Gyan Vihar University, Jaipur (Rajasthan) to study the effect of foliar application of boron and zinc on growth, yield and quality of guava (Psidium gaujava L.) during the year 2022-23. The various concentrations of boric acid, zinc sulphate and their interactions had significant effect on various vegetative growth, yield and quality parameters and the maximum (22.07 cm) tree height increment, (70.39 cm) annual shoot growth, (215.96) fruits per tree, longest (8.74 cm) fruit length, (7.84 cm) fruit diameter, heaviest (264.20 g) fruit, yield (58.57 kg/tree) and (16.27 t/ha), (15.87%) TSS, (7.60%) total sugar, (268.25 mg/100 g) ascorbic acid and (1.36%) pectin was recorded under Boric acid @ 0.25% + ZnSO4 @ 0.25% (T11).The non-significant effects were observed in application of different combinations of boric acid and zinc sulphate on acidity percentage.

Genome-wide transcriptome profiling of mulberry (Morus alba) response to boron deficiency and toxicity reveal candidate genes associated with boron tolerance in leaves

Mulberry (Morus alba) is an essential plant with countless economic benefits; however, its growth and metabolic processes are hampered by boron (B) stresses. Very little research has been performed to elucidate boron tolerance and detoxification mechanisms in this species. The M. alba cultivar, Yu-711, was exposed to five different concentrations of boric acid (H3BO3), including deficient (T1; 0 mM) moderate B deficiency (T2; 0.02 mM), sufficient (CK; 0.1 mM) and toxic (T3 and T4; 0.5 and 1 mM) levels for 18 days of growth in pots experiment. Transcriptome analysis of B deficiency and toxicity treatments was performed on mulberry leaves. The transcriptome data reveal that a total of 6114 genes were differentially expressed (DEGs), of which 3830 were up-regulated and 2284 were down-regulated. A comparative analysis between treatment groups CK-vs-T1 (deficiency) and CK-vs-T4 (toxicity) indicates that 590 and 1383 genes were down-regulated in both deficiency and B toxicity, respectively. The results show that 206 genes were differentially expressed in all treatments. B deficiency and toxicity significantly altered the expression of the key aquaporins (PIP2-1, PIP2-7, PIP2-4 and NIP3-1) and high-affinity boron transporter genes (BOR1 and BOR7). In addition, boron stress also altered the expression of antioxidants and photosynthesis-related genes. B stresses were found to alter several transcription factors including ERF1B, which is associated with the regulation of boron uptake and the synthesis and signaling of phytohormones. Unravelling the mechanisms of B tolerance and detoxification is important and would give us further insight into how B stresses affect mulberry plants.

Assessment of pollen quality and quantity in White and Black Turkish Myrtus communis L. accessions, through In Vitro pollen germination under varied boric acid concentrations

Assessment of pollen quality and quantity in White and Black Turkish Myrtus communis L. accessions, through In Vitro pollen germination under varied boric acid concentrations