Bloodstream Speed Research Articles

PB2364 UNSUCCESSFUL COLLECTION OF AUTOTRANSPLANT DURING THE FIRST SESSION OF LEUKOCYTAPHERESIS IN PATIENTS WITH MULTIPLE MYELOMA AND EFFECTIVE STEM CELL MOBILIZATION

Background:It is well known that the effectiveness of stem cell collection and the possibility to harvest suboptimal CD34+ cells number during the single apheresis session is dependent on the number of CD34+ cells circulating in the peripheral blood (PB), i.e. on the effectiveness of mobilization regimen.Aims:To determine the rate of unseccessful collections in patients with multiple myeloma (MM) when the number of CD34+ cells circulating in the PB is in accordance with the optimal level 20/1 mkl or more.Methods:Retrospective analyses of autotransplant collection in 55 MM patients have been done. Median age of patients was 56 (31‐67) years. Complete response, very good partial response and partial response were diagnosed in 12 (21.8%), 13 (23.6%) and 30 (54,5%) patients accordingly. 25 (45.5%) patients were treated with lenalidomide previously. Cyclophosphamide 3 g/m2 with granulocyte colony‐stimulating factor (G‐CSF) were used for stem cell mobilization in 28 (50.9%) patients. 26 (47.3%) patients were mobilized with vinorelbine 35 mg/m2 with G‐SCF and G‐SCF monotherapy was used in 1 (1.8%) patient.Results:The number of CD34+ cells circulating in PB at the day of the first apheresis procedure was 5‐694/1 mkl (Me 118). Median number of collected CD34+ cells was 5.53 × 10/6/kg (0.27‐29.23). The correlation between number of circulating CD34+ cells and the quantity of harvested CD34+ cells was confirmed: r = 0.503; p < 0.05. At the same time there were 7 (12.7%) cases in which the number of collected CD34+ cells were lower than suboptimal level (0.25‐1.9 × 10/6/kg, Me 1.33 × 10/6/kg) despite the successful mobilization rate: 26‐118/1 mkl (Me 44). The second procedure of apheresis was done on the next day and in all cases except for the one there was the possibility to harvest CD34+ cell number enough to perform a single stem cell transplantation: 2.58‐3.48 × 10/6/kg. The place of central veun catheterization has been changed in all these cases except for the one.Summary/Conclusion:We suppose that a reason of unseccessful stem cell collection in MM patients with adequate rate of mobilization may be disturbance of the bloodstream speed in the apheresis system due to the anatomical characteristics of subclavia vein localization. A method to correct the problem is relief the central catheter puncture place.

КЛИНИЧЕСКАЯ ОЦЕНКА ЭФФЕКТИВНОСТИ КОМПЛЕКСНОГО ЛЕЧЕНИЯ ПАЦИЕНТОВ С ХРОНИЧЕСКИМ КАТАРАЛЬНЫМ ГИНГИВИТОМ С ПРИМЕНЕНИЕМ ИНЪЕКЦИОННОГО ГЕЛЯ ГИАЛУРОНОВОЙ КИСЛОТЫ

Methodology. 48 somatically healthy patients of both gender in age from 25 up to 45 was examined, that included questioning, examination, index estimation of the state of fabrics of paradontium, doppler ultrasound of vessels of paradontium with the use of device of "Minimax-Doppler-K" NB model with the sensor 20 Mhz. Linear speed of blood stream, volume speed of blood stream were defined. Patients were divided into 3 groups: 1 - basic group (n=22) are patients with chronic catarrhal gingivitis where the standard treatment was complemented by application of injections of gel, containing 1 % of hyaluronic acid and 0,015 % of hydrophilic polysaccharide of AGEG, under a mucous membrane on a mucogingival fold; 2 is a control group (n=16) patients with chronic catarrhal gingivitis and the standard treatment (in accordance with clinical recommendations) was complemented; 3 group are people with a clinically healthy paradontium (n=10). Dynamic estimation of efficiency of treatment was conducted on the 2 day and 7th day, 14th day, in 1 month, 3 months.Results. There was a stable reduction of PMA index in a basic group, that shows the efficiency of treatment and remission in the period of 3 months observation. More low level of PBI and PI (p<0,05) indexes was determined statistically meaningfully in a basic group, according to the results of 12 weeks of supervision.. 
 Results. The indexes of hemodynamics had more obvious tendency to approach descriptions of intact paradontium.

Indices’ Dynamics of Microcirculatory Processes in Women with Habitual Miscarriage of Pregnancy Daily Wearing Prophylactic Trousers in the Course of the Third Pregnancy Term

It is acknowledged at present that habitual miscarriage of pregnancy is accompanied by disturbances of hemo-circulation process in vessels of little caliber. In our research we applied the author’s variant of medicinal-prophylactic trousers’ daily wearing in the course of the third term in women with habitual miscarriage of pregnancy who received traditional saving pregnancy therapy. In our research we applied instrumental, laboratory and statistical methods of investigation. The women wearing medicinal-prophylactic trousers were noted to have the improvement of microcirculation indices in lower extremities. It became possible in the result of the increase of linear systolic bloodstream speed by more than 16.4%, linear average bloodstream speed by more than 50.0%, and average volumetric bloodstream speed by more than 78.0% and level decrease of systolic bloodstream speed by more than 38.0%. The plasma of women with habitual miscarriage of pregnancy wearing medicinal-prophylactic trousers in the course of the third term, was reached to have leveling of existing imbalance of arachidonic acid metabolites on behalf of level decrease of thromboxane B2 and level rise of 6-keto-prostaglandin F1α till the values of the norm. It was accompanied by the rise of nitric oxide summary metabolites nearly till the control level in plasma of the examined women by the end of observation. Daily wearing of medicinal-prophylactic trousers was accompanied by content rise of erythrocytes-discocytes in blood of patients till 85.5 ± 0.18% at the decrease of erythrocytes’ reversibly and irreversibly modified forms till 10.9 ± 0.07% and 3.6 ± 0.04%, respectively. At the same time the group of observation reached normalization of erythrocyte aggregation on behalf of erythrocytes’ sum decrease in aggregates by 30.8% and quantity of these aggregates-by 29.2% at number increase of non-aggregated erythrocytes by 20.4%. So, at wearing of medicinal-prophylactic trousers in the third term the women with habitual miscarriage of pregnancy reach potentiation of the conducted therapy with normalization of microcirculation indices and erythrocytes’ rheological properties. Given alterations can optimize the processes of microhemodynamics and activate trophism in the field of small pelvis promoting successful carrying a baby to full term.

Results of clinical approbation of new local treatment method in the complex therapy of inflammatory parodontium diseases

Treatment and prevention of inflammatory diseases of parodontium are one of the most difficult problems in stomatology today.Purpose of research: estimation of clinical efficiency of local combined application of developed agent apigel for oral cavity care and low-frequency electromagnetic field magnetotherapy at treatment of inflammatory diseases of parodontium.Materials and methods: 46 patients with chronic generalized catarrhal gingivitis and chronic generalized periodontitis of 1st degree were included into the study. Patients were divided into 2 groups depending on treatment management: basic (n = 23) and control (n = 23). Conventional treatment with the local use of the dental gel with camomile was used in the control group. Patients of the basic group were treated with local combined application of apigel and magnetotherapy. Efficiency was estimated with clinical, laboratory, microbiological and functional (ultrasonic Doppler examination) methods of examination.Results: The application of the apigel and pulsating electromagnetic field in the complex medical treatment of patients with chronic generalized periodontitis (GhGP) caused positive changes in clinical symptom and condition of parodontal tissues, that was accompanied by decline of hygienic and parodontal indexes. As compared with patients who had traditional anti-inflammatory therapy, patients who were treated with local application of apigel and magnetoterapy had decline of edema incidence. It was revealed that decrease of the pain correlated with improvement of hygienic condition of oral cavity and promoted prevention of bacterial contamination of damaged mucous membranes. Estimation of microvasculatory blood stream with the method of ultrasonic doppler flowmetry revealed more rapid normalization of volume and linear high systole, speed of blood stream in the parodontal tissues in case of use of new complex local method.Conclusions: Effect of the developed local agent in patients with inflammatory diseases of parodontium restore gingival sulcus microbiocenosis and structural-functional state of parodontium.

Oxidative modification of proteins of blood plasma in the development of ovarian cancer relapse.

e22002 Background: Comparative analysis of oxidative modification of proteins of blood plasma level in patients with ovarian cancer in a state of remission and during the formation of tumor relapse with various intensity of bloodstream in it. Methods: The research includes 61 patients with ovarian cancer, from whom 11 women were in a state of remission, and 50 had local relapse in the cavity of the lesser pelvis. With the help of ultra-sonic scanner we evaluated the intensity of vascularization and the speed of bloodstream in relapsing tumors. We also studied the level of blood plasma spontaneous metal catalyzed oxidative modification of proteins (OMP), spectrophotometrically registering 2, 4-dinitrophenylhydrazines. Results: In remission there was noted the increase of OMP products level by 74.4% under 530 nm (p<0.05) in blood plasma as compared with the its level in healthy women. During the progress of avascular relapse there was noted the increase of OMP products level by 63.3% under 370 nm (p<0.001) and decrease of OMP products level by 32.2% under 530 nm as compared with the level in remission. In the group with hyper intensive bloodstream in relapse tumor the level of OMP products increased by 37.4% under 370nm (p<0.05) and the level of products decreased by 24% under 530 nm as compared with the group with avascular relapse. In case with metal catalyzed OMP the low level of OMP products under 370 nm (by 18-28% lower than the level of OMP of healthy women, p<0.01), the high level of OMP products under 530 nm (by 24-47% higher than the level of OMP of healthy women, p<0.01) remained, and the level of OMP under 430 nm was increasing as compared with healthy women. Conclusions: The increase of OMP products of neutral character registered under 370 nm, and the decrease of the products of the basic character registered under 530 nm take place during the progress of cancerous process and intensification of the bloodstream in a tumor. The dynamics metal catalyzed OMP products shows the heightened formation of the products of the basic character (430 and 530 nm), being the markers of proteins fragmentation.

Influences of early hyperbaric oxygenation on postoperative recovery of intracranial aneurysms patients

Objective To explore the effects of early hyperbaric oxygenation (HBO) on postoperative recovery of intracranial aneurysms patients.Methods The chnical data of 120 postoperative intracranial aneurysms patients were divided into HBO group and control group.The determination of average blood stream speed of middle cerebral artery(MCA) of operation side were recorded at the 1st,3th,7th,14th and 21st day after operation.The operative regional abnormity density volume of CT re - examination were calculated at the 1st,7th,14th and 21st day.The number of cases associated with “symptomatic cerebral vasospasm” was counted at the 3st,7th and 14st day.The functional state ( KPS score) was evaluated at the 3th and 21st day.Result There was no significant difference of MCA average blood stream speed,number of cases associated with “symptomatic cerebral vasospasm” and KPS score between two groups at 3rd day after operation( P ＞ 0.05 ).The MCA average blood stream speed of HBO group was lower than that of control group at the 7th and 14th day after operation ( P ＜ 0.05 and P ＜ 0.01 ).The abnormity density volume of HBO group was less than that of control group at the 14th and 21st day after operation ( P ＜ 0.05 ).The number of cases associated with “symptomatic cerebral vasospasm” of HBO group was smaller than that of control group at the 7th and 14th day after operation ( P ＜0.05).The KPS score of HBO group was higher than that of control group at the 21 st day after operation ( P ＜ 0.05 ).Conclusion The effects of early hyperbaric oxygenation therapy on postoperative intracranial aneurysms patients are good.One of the possible mechanism is release of cerebral vasospasm. Key words: Intracranial aneurysm ; Cerebral vasospasm ; Hyperbaric oxygen therapy ;

Arterial embolization in the therapy of prostatic hyperplasia (a report of 12 cases)

目的 探讨动脉栓塞治疗良性前列腺增生(BPH)的方法及疗效.方法 经穿刺活检确诊BPH患者12例,行动脉造影,确定为前列腺供血动脉后,经导管注入聚乙烯醇(PVA)和明胶海绵颗粒进行栓塞.对患者手术前后国际前列腺症状评分(I-PSS)、生活质量评分(QOL)、最大尿流率(Qmax)、剩余尿(RU)进行比较,并经直肠超声、CT观察前列腺大小、血流变化以评价疗效.结果 对12例患者的21支前列腺优势供血动脉进行了栓塞治疗.栓塞的前列腺供血动脉为髂内动脉5支、膀胱下动脉9支、阴部内动脉5支、闭孔动脉2支.单侧栓塞3例,双侧栓塞9例.术后I-PSS、QOL、Qmax、RU,从术前的24.2分、4.8 分、9.6 ml/s、138 ml,分别降至4.8分、1.3分、 18.9 ml/s、3 ml;术后彩色多普勒监测前列腺内血流信号明显减弱、最大血流速度从术前的(19.3±9.8) cm/s减慢到(10.0±8.9) cm/s; B超或CT检查前列腺体积从术前的平均127.6 ml 缩至65.1 ml,缩小51%.结论 动脉栓塞治疗BPH是一种创伤小、疗效好、安全性强,并发症少的新方法,具有良好的临床应用价值。

Approach of improving precision in ultrasonic Doppler bloodstream speed measurement by chaos-based frequency detecting

It is critical for cerebral vascular disease diagnosis through Doppler to detect the maximum and the minimum of the carotid blood flow speed accurately. A kind of Duffing system under an external periodic power with dump is introduced in the letter, numerical analysis is carried out by four-order Runge-Kutta method. An oscillator array is designed according to the frequency of the ultrasonic wave. When the external signals are inputted, computational algorithm is used to scan the array in turn and analyze the result, and the frequency can be determined. Based on the methods above, detecting the carotid blood flow speed accurately is realized. The Signal-to-Noise Ratio (SNR) of −20.23dB is obtained by the result of experiments. In conclusion, the SNR has been improved and the precision of the measured bloodstream speed has been increased, which can be 0.069% to 0.13%.

Chaos weak signal detecting algorithm and its application in the ultrasonic Doppler bloodstream speed measuring

At the present time, the ultrasonic Doppler measuring means has been extensively used in the human body's bloodstream speed measuring. The ultrasonic Doppler measuring means can achieve the measuring of liquid flux by detecting Doppler frequency shift of ultrasonic in the process of liquid spread. However, the detected sound wave is a weak signal that is flooded in the strong noise signal. The traditional measuring method depends on signal-to-noise ratio. Under the very low signal-to-noise ratio or the strong noise signal background, the signal frequency is not measured. This article studied on chaotic movement of Duffing oscillator and intermittent chaotic characteristic on chaotic oscillator of Duffing equation. In the light of the range of the bloodstream speed of human body and the principle of Doppler shift, the paper determines the frequency shift range. An oscillator array including many oscillators is designed according to it. The reflected ultrasonic frequency information can be ascertained accurately by the intermittent chaos quality of the oscillator. The signal-to-noise ratio of −26.5 dB is obtained by the result of the experiment. Compared with the tradition the frequency method compare, the dependence to signal-to-noise ratio is lowered consumedly. The measuring precision of the bloodstream speed is heightened.