Proteins In Blood Plasma Of Patients Research Articles

Dynamics of oxidative modification of blood plasma proteins in patients with acute epididymitis under the influence of treatment

У нашому дослідженні була виконана спроба з'ясування діагностичної та прогностичної значимості рівня окисної модифікації білків (ОМБ) у плазмі крові хворих на гострий епідидиміт (ГЕ) протягом лікування. У всіх обстежених пацієнтів до лікування виявили підвищення рівня окисної модифікації білків. У період надходження до стаціонару у всіх хворих на ГЕ рівні ОМБ виявились високими, що свідчить про високий окисний стрес при даному захворюванні. Протягом лікування ці показники поступово знижувалися до норми. Отримані дані можуть бути використані як для моніторингу лікування, так і одужання хворих.

396 - Oxidative and Nitrative Modifications of Blood Plasma Proteins in Patients with Myasthenia Gravis

Myasthenia gravis (MG) is an autoimmune disease caused by production of antibodies against acetylocholine receptors of the neuromuscular junction (Ab). As oxidative damage is involved in inflammatory and autoimmune-mediated diseases, we studied oxidative modifications of blood plasma proteins in patients with MG. The group studied consisted of 50 MG patients, 24 with ocular and 26 with generalized MG (28 females and 22 males), mean age 66.7 (30-81) y, mean duration of disease 9.5 (0.5-34) y, mean level of Ab of 8.9 (0.1-85) nmol/ml, and 25 age-matched healthy controls. The patients were classified according to the type of MG (Osserman's classification), duration of disease ( 5 y) and Ab level (low, 3 nmol/l). Glycophore fluorescence was increased in ocular MG a . Dityrosine was increased in both types of MG c , in patients ill b and >5 c y, with low c and high c levels of Ab. N-formylkynurenine was increased in the ocular a and generalized b MG, in both disease duration groups a , in the group of low Ab a . Kynurenine was incre a sed in the group with high Ab a . Tryptophan fluorescence was decreased in ocular b and generalized a MG, in patients ill for b and >5 a y, with low a and high c A b . Thiol groups were decreased in ocular a and generalized c MG, in patients ill for >5 a y, in the group with high Ab a . AOPP was elevated in p a tients with high Ab a . Protein carbonyls were increased in both ocular b and generalized b MG, in patients ill for >5 b y, with low b and high b A b . FRAP and ABTS due to fast antioxidants were unchanged but ABTS due to slow antioxidants was lower in ocular c and generalized b MG, in patients ill for >5 c y, in patients with low c and high b A b ( a P b P c P

Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection

Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection

Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection.

Blood plasma proteins of patients with Alzheimer's disease (AD; senile dementia) and non-AD-type dementia were resolved by two-dimensional electrophoresis and identified by migration position in the electrophoresis pattern, sequence homology, and immunodetection by using antibodies. For the control experiments, blood plasma proteins of a healthy young individual and non-dementia patients were examined in a manner similar to that of the plasma samples of AD patients. In the plasma sample of the healthy young individual, more than 350 spots of silver-stained proteins were observed and among these spots, 73 spots were identified. Blood plasma proteins of the AD and non-AD-type dementia patients were compared with those of the control and non-dementia patients. In the blood plasma samples of five AD patients, three patients had apolipoprotein E4, and another patient showed apolipoprotein L and complement factor H. For the AD-related proteins apolipoprotein E, tau-1, and presenilin 2, proteins were examined by immunostaining with antibodies, in both AD and non-AD patients. Among the three samples of non-AD-type dementia patients, one was distinguishable by amyloid A proteins, and the other by haptoglobin isoforms.

Blood plasma proteins in patients with heart failure: Heirmann, G. R.: Ann. Int. Med. 24: 893 (May), 1946

Blood plasma proteins in patients with heart failure: Heirmann, G. R.: Ann. Int. Med. 24: 893 (May), 1946