Abstract. The purpose of the study is to study the effect of bedding material on the physiological parameters of broiler chickens. Since the most important condition for optimizing the microclimate in the poultry house is compliance with the physiological state of the livestock and maintaining its vital activity at an optimal level. Research methods. The experiment was carried out in the industrial conditions of a poultry farm on broiler stock of the Arbor Acres+ cross in the experimental workshop, where four sections were formed. The growth and development of broilers was monitored daily, livestock records were carried out, and a weekly veterinary report was compiled on the causes of mortality. Also, laboratory studies were carried out according to approved methods for the following indicators: ammonia content, sanitary and hygienic indicators of bedding material, volume, weight and moisture content of bedding material, as well as productivity and basic blood parameters of broiler chickens. Results. Trends in the positive effect of the sorbent in the form of modified diatomite on the productivity of broiler chickens due to the improvement of zoohygienic growing conditions have been identified. In particular, the content of the total number of microorganisms, mold spores and ammonia content in the production experimental workshop is examined in detail. During the work, the volume and weight of bedding material was calculated, including at the exit from the poultry house. We tried to pay special attention to blood biochemistry, which made it possible to assess the physiological state of broiler chickens and draw conclusions about the quality of feeding rations. At the same time, problematic issues associated with feeding poultry were identified, which affect the content of mineral elements in the blood. When determining the performance criterion, calculations were carried out using the EPEF index. Scientific novelty. For the first time, it was proven that the use of diatomite with a bulk layer height of 0.5–1 cm with a granule size of at least 5 mm as bedding material leads to improved zoohygienic conditions for raising broiler chickens without additional capital investments in the form of installation of expensive ventilation equipment or the use of additional technological operations. This replacement option has a positive effect on the health of young poultry.