The purpose of the work was to conduct a scientific and experimental analysis of the study of the functional state of the organism of hydrobionts while growing in recirculation of aquaculture systems using an unconventional method of water treatment, which was used in the formation of feed forage for their feeding. The experimental part of the work was carried out on the basis of the laboratory of aquatic bioresources and aquaculture of the Biotechnology Faculty of the Dnipro State Agrarian and Economic University and the Scientific and Experimental Center “Aquatic Biodiversity and Aquaculture of the Pre Dnieper” of the Dniprovsky State Agrarian and Economic University. The practical part of the work consisted of several stages: the formation of the preparatory period (obtaining the breeding culture of phytoplankton and zooplankton, cultivation on different environments in order to establish an optimal scheme); obtaining of fish planting material and determination of fish objects for experiment; staging of the experiment (conducting research on feeding natural food of different processing of mother-crops). Scientific and experimental studies on the study of the functional state of the organism of hydrobionts when cultivated in the PAC with the use of an unconventional method of processing fodder mixed with their feeding give grounds to note the positive impact on its qualitative and functional characteristics. Investigation of the morpho-functional status of blood of freshwater fish on the background of the use of the proposed method for processing fodder mixed with fertilization in the early stages of ontogeny showed that stimulation of erythropoiesis, activation of protein metabolism in the organism of hydrobionts within the limits of the physiological norm occurs in the experimental group. The combination of several rapid methods (GRU) makes it possible to provide comprehensive research and acquires scientific and practical value, since the study of the physiological state of the object of biotesting against the background of the use of various factors involves the implementation of an entire chain of sequential research.