Blood Group Research Articles

Anemia in Reproductive Age Group Women Attending in General Health Check-up in the Tertiary Care Centre in Kathmandu, Nepal: A Descriptive Cross-sectional Study

Background: Anemia is common, particularly among women in Southeast Asia and other Asian countries. In reproductive-age women (15-49 years), increased demand without adequate supply often leads to deficiency. This study aimed to determine the prevalence of anemia in this group. Methods: A descriptive cross-sectional study was conducted among women of reproductive age (15-49 years) visiting Tribhuvan University Teaching Hospital August 1st 2024 to October 20th 2024, following ethical approval. Exclusions included those with chronic diseases, incomplete data, heavy menstrual loss, or pregnancy complications. A convenience sampling method was used, yielding 372 participants. Data were recorded in MS Excel and analyzed in SPSS. Results: Our study of 372 participants (mean age 35.39 ± 8.73 years) found that 22.8% were women aged 40-44 years. BMI analysis showed 43% were overweight, and 34.1% had normal BMI. Blood group distribution was 27.4% A+ and 28.8% B+, with A- being the least common (0.3%). Most participants (75.3%) were from the Hill region, and 71.8% were unmarried. Anemia prevalence was 24.46%, highest in the 45-49 age groups and lowest in the 20-24 groups. Anemia was more common in the underweight (44.44%) and very obese (50%) groups and most prevalent among Terai region women (35.59%). Conclusion: The study found a significant prevalence of anemia among women of reproductive age, with higher rates observed in specific age groups, BMI categories, and geographic regions. This highlights the need for targeted interventions to address anemia in vulnerable populations.

Біометричний аналіз показників прооксидантно-антиоксидантного стану плазми крові щурів за дії гістаміну і кверцетину

The regularities of the effect of histamine in concentrations of 0.01; 0.1; 1; 10 μM were studied and quercetin in concentrations of 0.1; 0.3; 0.5; 1; 3; 5 mM, as well as their combined effect on the prooxidant-antioxidant state of blood plasma of rats, using cluster and factor biometric analyses. It was established that the experimental groups according to the investigated indicators (TBА-positive products, lipid hydroperoxides, carbonyl groups of neutral and basic proteins, superoxide anion radical, superoxide dismutase, catalase, reduced glutathione, ATP) were distributed among 13 clusters (according to cluster analysis). In one group of similarities, the effect of histamine in concentrations of 0.01 µM and 1 µM was revealed. Quercetin at a concentration of 0.5 mM and histamine at a concentration of 0.1 µM have a similar effect on the indicators of the prоoxidant-antioxidant state of the blood plasma. Combined addition to the blood of histamine at a concentration of 10 μM and quercetin at a concentration of 0.1; 0.5; 3 mM lead to the same changes in the indicated studied indicators. Cluster analysis also combined the combined effects of 0.01 μM histamine and 0.1 and 3 mM quercetin. It is important to note that a decrease in the content of carbonyl groups of proteins was found in those classers whose experimental blood groups were added to histamine and quercetin, which indicates a decrease in protein damage due to free radical oxidation processes. Using factor analysis, it was established the presence of three hidden factors that affect the processes of free radical oxidation of blood under the action of histamine and quercetin. A high correlation of factor I with protein carbonyl groups, reduced glutathione, superoxide anion radical was revealed. Factor II is most correlated with ATP, superoxide dismutase, lipid hydroperoxides. There is a close relationship between factor III and TBА-positive products and catalase. Taking into account the closeness of the relationship, factor I was given the name “factor of action on proteins”, factor II – “factor of action on bioenergetics and initiator of lipid peroxidation processes”, factor III – “factor of enhancement of lipid peroxidation processes”. It was established that quercetin activates factor I (influence on proteins, causing their oxidation) and factor III (intensification of lipid peroxidation processes) depending on the concentration of the drug. Quercetin in a concentration of 1 mM has the property of both factors (I and III).

Blood Management System Using Java with GUI and Array List-based Database

This research paper presents the development of a Blood Management System (BMS) using Java with a graphical user interface (GUI), ArrayList as a data structure for managing records, and file handling for persistence. The system provides an efficient and user-friendly platform for blood donation management, focusing on functionalities accessible to an administrative user (admin) only. Key features include donor data management (adding, updating, searching, deleting), stock management of blood groups, and essential application controls like login/logout and exit functionalities. This system aims to streamline the management of blood banks by providing quick access to critical information such as donor location, blood group, and stock availability. The source code for the Blood Management System is accessible at https://github.com/BloodBankManagment.

Integrated Health Monitoring System using Fingerprint and Disease Risk Prediction

Recent developments in machine learning and biometric systems have had a big influence on healthcare applications. In order to provide a holistic approach to health monitoring, this research introduces a unique method that combines disease risk prediction with fingerprint-based blood group recognition. Based on user-provided health data, the system uses machine learning algorithms to determine the risk of common illnesses including diabetes, cardiovascular disease, and chronic kidney disease (CKD) and convolutional neural networks (CNNs) to identify blood type from fingerprint photos. Through the integration of fingerprint biometric data with health risk modelling, the suggested approach seeks to increase the precision and usability of health forecasts. The system's effectiveness is demonstrated by the evaluation's excellent classification accuracy for blood groups and trustworthy estimates of illness risk.This work demonstrates the possibility of integrating biometric data with predictive health models and offers a promising step toward customized healthcare solutions

Preparation and performance study of heparinized multi‐walled carbon nanotube/cellulose acetate hemodialysis membrane

AbstractCurrently, the incidence of kidney failure is increasing worldwide. Hemodialysis is the main method for the treatment of kidney diseases, and the continuous development of new hemodialysis membranes is the key to improve the treatment effect and the quality of life of patients. In this study, heparinized multi‐walled carbon nanotube/cellulose acetate (Hep/MWCNTs@CA) membranes were prepared by non‐solvent induced phase separation (NIPS). The morphology and pore structure of Hep/MWCNTs@CA membranes were characterized by scanning electron microscopy (SEM). The elongation at break and tensile strength of the membranes were characterized by a mechanical property universal tester. The results showed that mechanical properties of 0.1 wt% MWCNTs enhanced the membrane is best. The water contact angle test confirmed that MWCNTs can improve the membrane hydrophilicity, and combined with the adsorption test results for proteins, it was found that the hydrophilicity can reduce the adsorption of proteins to the composite membrane and improve the blood compatibility of the material. The dialysis performance of urea and lysozyme was tested by a self‐made simulated hemodialysis device, and the clearance rates of urea and lysozyme were 84% and 47.2%, respectively, which showed that the interfacial gap between MWCNTs and CA matrix can make the composite membrane retain a high retention rate of macromolecular proteins (92.3%) and further improved the clearance rate of small and medium molecular toxins. In addition, the good blood compatibility and biocompatible properties of the materials were confirmed by hemolysis, recalcification and cytotoxicity tests on Hep/MWCNTs@CA membranes. In conclusion, the prepared Hep/MWCNTs@CA holds great potential for application in the field of hemodialysis.

Abstract A013: Quality control of the biobanked matched tissue: Blood samples for the development of diverse liquid based molecular assays

Abstract The availability of high-quality matched biological samples (tumor-blood) is the major limiting factor for research and development of novel, liquid biopsy-based assays in oncology. Frequently, ongoing specifically timed collections of blood samples are required. De-identified tissue blocks (formalin-fixed, paraffin embedded (FFPE) or flash frozen) matched with patient's blood samples collected in fixative-containing (Streck) of EDTA containing tubes, were collected at worldwide geographic locations and transferred to biobanking facility (Reference Medicine, Phoenix, AZ). Additional blood samples were collected at various times regarding the cancer surgery. Plasma and buffy coat samples were locally prepared and shipped to the biobank. A subset of blood samples without matched tissues from healthy donors were also collected. All samples were shipped under strict transport conditions. Quality control performed at the biobanking facility included pathology review and nucleic acids quality assessment. A total of over 3,000 cases were collected and provided to end-users working on various assays based on cfDNA (e.g. monitoring for minimal residual disease or multi-cancer early detection). Immuno-reactivity to various antigens in samples collected in Streck and EDTA tubes (from 50 patients) was assessed by peptide microarray binding assay. Feedback information on the quality of samples was shared in 900 cases. Approximately 4% of cases had failed internal pathology review, due to poor preservation, lack of adequate tissue composition, or low tumor volume and 11% failed internal nucleic acid quality assessment (DNA concetration and integrity). The blood collection tubes had no significant impact on serologic testing using peptide microarary assay. Customer feedback data showed that overall success rate exceeded 85%. Type of blood collection tubes (Streck vs EDTA) had no impact on serologic testing and studies looking at predicted neoantigens based on NGS data can be confirmed using peptide epitope arrays. Blood collected for NGS works without compromise on peptide arrays. Future validation of other collection tubes for blood or other types of fluids is needed to confirm their adequacy for specific assays. Citation Format: Zoran Gatalica, Phillip Stafford, Chris Diehnelt, Inga Rose. Quality control of the biobanked matched tissue: Blood samples for the development of diverse liquid based molecular assays [abstract]. In: Proceedings of the AACR Special Conference: Liquid Biopsy: From Discovery to Clinical Implementation; 2024 Nov 13-16; San Diego, CA. Philadelphia (PA): AACR; Clin Cancer Res 2024;30(21_Suppl):Abstract nr A013.

Red cell specifications for blood group matching in patients with haemoglobinopathies: An updated systematic review and clinical practice guideline from the International Collaboration for Transfusion Medicine Guidelines.

Red blood cell (RBC) antigen matching beyond ABO and RhD is commonly recommended for patients with sickle cell disease (SCD) and thalassaemia. We present an updated systematic literature review to inform evidence-based guidelines on RBC matching. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) tool was used to develop recommendations. Six new observational studies (4 prospective, 2 retrospective) were identified. The six studies reported on 583 patients in total, including cross-over designs, with sample sizes from 10 to 343. Studies were heterogeneous, utilising varying degrees of RBC matching and different definitions for 'extended' matching. All reported on alloimmunisation. One study reported on molecular matching. The reported prevalence of alloimmunisation using limited matching was 0%-50% and with extended matching was 0%-24%. Eighty-two patients were alloimmunised before study entry. The risk of bias across studies was moderate to critical. The guideline panel recommends that ABO, RhDCcEe, and K-compatible RBCs are selected for individuals with SCD and thalassaemia, even in the absence of alloantibodies, and that RBCs which are antigen-negative to already existing clinically significant antibodies are chosen. There is a need for comparative research to define the benefit, impact, cost-effectiveness, and feasibility of extended RBC matching strategies to prevent alloimmunisation.

IMMUNOLOGICAL REACTIVITY AND REACTIVE RESPONSE OF PERIPHERAL BLOOD NEUTROPHILIC GRANULOCYTES IN PATIENTS WITH COVID-19 DEPENDING ON THEIR BLOOD GROUP

At the onset of the Coronavirus Disease 2019 (COVID-19) pandemic, a potential link between ABO blood type and susceptibility to the disease was reported. The evidence supporting this link is strengthening as the volume and quality of research expand. Recently, interest has shifted from merely examining susceptibility to exploring the severity of the disease and the immune response characteristics of patients in relation to their blood groups. Objective: to investigate the immunological reactivity and reactive response of peripheral blood neutrophilic granulocytes in patients with COVID-19 patients based on their blood group. Materials and methods. This prospective study included 198 patients with COVID-19-associated pneumonia of mild, moderate, and severe degrees. The cohort comprised 50.51% (100) men and 49.49% (98) women, with an average age of 54.18 ± 7.25 years (range: 18 to 80 years). The distribution of blood groups according to the ABO system was as follows: 0 (I) - 37.37% (74 patients), A (II) - 37.37% (74 patients), B (III) - 18.18% (36 patients), and AB (IV) - 7.07% (14 patients). The immunological reactivity and reactive response of neutrophilic granulocytes were assessed through the analysis of 14 integral leukocyte indices, including the leukocyte shift index, the ratio of the absolute number of leukocytes to the erythrocyte sedimentation rate (ESR), the lymphocyte-granulocyte index, the lymphocyte index, the index of immunological resistance, and reactivity, among others. Results. In moderate and severe coronavirus infection, the indices of cellular and total immune reactivity and resistance are significantly lower compared to mild coronavirus infection: 1.35-3.08 times (p≤0.042-0.001) for resistance, 2.02-3.87 times (p<0.001) for reactivity, respectively, showing the highest activity among them in the owners of blood group 0 (I), with significantly lower values for A (Ⅱ), B (Ⅲ) and AB (Ⅳ) group variants by 3.94-19.10%. Whereas, with increasing severity of the disease, the reactive response of neutrophilic granulocytes, on the contrary, increases significantly compared to the mild degree: in moderate disease by 43.79-87.62% (p≤0.018-0.005), in severe disease by 1.62-2.11 times (p≤0.012-0.001), with the highest average value in AB (Ⅳ) blood group owners – by 46.69-68.44% (p≤0.017-0.005). Conclusions. The highest index of immunological reactivity is observed in individuals with blood group AB (IV) experiencing a mild clinical course of the disease. Conversely, the lowest index of immunological reactivity is found in patients with blood group A (II) who have a moderate clinical course. Additionally, the reactive response of neutrophilic granulocytes significantly increases in patients with a severe clinical course, with the highest average value recorded in those with blood group AB (IV).

Pancreatic cancer – challenge for modern medicine

Introduction. Pancreatic cancer is one of the most dangerous carcinomas of the digestive tract, with a very poor prognosis for patients. Annually, more than three and a half thousand people in Poland are diagnosed with this tumor. Aim. The aim of the work was to assess and compile existing knowledge about pancreatic adenocarcinoma. Materials and methods. Data for the paper was gathered with the usage of Google Scholar. Moreover, articles from sources like PubMed or National Institutes of Health were used. Materials were published between 2015 and 2024. Results. Tumor morbidity is still increasing, the biggest group of patients are people who are more than 55 years old. Many modifiable and nonmodifiable factors play a role in the pathogenesis of pancreatic cancer. Lifestyle, stimulants, BMI or physical activity can be included into the first group. Nonmodifiable factors are sex, age, race, genetic predisposition or blood group. Typical symptoms of this tumor are loss of the body weight, fatigue, nausea, jaundice and abdominal pain. Clinicians use the following diagnostic methods: CT, MRI, ERCP, MRCP or USG. Only the radical chirurgical treatment gives a hope for total recovery, additionally chemotherapy can be admitted. Chemotherapy schemes are FOLFIRINOX or monotherapy with gemcitabine. Conclusions. Pancreatic cancer is still a challenge for clinicians, as its treatment is limited by poor effectiveness of possible ways of treatment. The majority of tumors are diagnosed at the advanced stage, when full patient recovery is not possible. New ways of treatment such as immunotherapy, monoclonal antibodies or gene therapy are a ray of hope. Keywords: pancreatic cancer, treatment of pancreatic cancer, epidemiology, diagnostic methods.

Stratification of Hand Bone Fractures in the Kyrgyz Republic

The study aimed to stratify hand bone fractures in the Kyrgyz Republic based on patient data, including age, gender, and other clinical characteristics. Data from patients treated in 2019 were analyzed, with assessments made using key medical parameters such as fracture type, age, gender, blood group, and other laboratory indicators. A retrospective analysis of 200 hand fracture cases was conducted, classified by various clinical and demographic criteria. Conclusions were drawn based on statistical analysis of the data. Results: Significant differences in fracture incidence were observed between males and females, as well as across different age groups. The highest incidence was found in the working-age population, indicating a need for enhanced preventive measures in this demographic.

Genetic analysis of a blood donor with combined FUT1 and ABO dual blood group gene variants resulting in para-Bombay and A2 subtype blood types and a literature review

To investigate the serological and molecular genetic characteristics of a voluntary blood donor with combined FUT1 and ABO blood group gene variants causing para-Bombay and A2 subtype, and to review relevant literature on para-Bombay blood types carrying alleles such as FUT101W.37 and FUT101W.23. A blood donor with para-Bombay and A2 subtype who participated in voluntary blood donation at the Dongguan Blood Center in August 2023 was selected as the study subject. Serological tests were performed to identify the ABO blood group, Lewis blood group antigens, and unexpected serum antibodies in the donor. Adsorption-elution test was conducted to detect trace antibodies in the blood donor's plasma to trace the A, B and H antigens on the red blood cell surface. Sanger sequencing was carried out to analyze the sequences of the FUT1 and ABO genes. Using keywords such as "para-Bombay" "FUT1*01W.37" and "FUT1*01W.23" both in Chinese and English, relevant literature on para-Bombay blood type subjects carrying FUT1*01W.37 and FUT1*01W.23 alleles was retrieved from the CNKI, Wanfang Data Knowledge Service Platform, and PubMed databases, and the retrieval time was set as from the establishment of database to December 2022. This study has been approved by the Ethics Committee of Dongguan Blood Center (No. 2022005), and informed consent of blood donation was obtained from the blood donor. Serological testing of the blood donor revealed inconsistent results between forward and reverse ABO blood typing, negative H antigen on the red blood cell surface, Le(a-b+) secretor type for Lewis blood group, and unexpected anti-H antibodies in the plasma, indicating a suspected para-Bombay type. Absorption-elution test suggested the blood type of the blood donor to be para-Bombay and A subtype. Sanger sequencing showed that the donor has harbored homozygous FUT1*(c.35T+c.803A)/(c.35T+c.803A) variant, with the FUT1*(c.35T+c.803A) allele containing a dual nucleotide variant unrecorded by the International Society of Blood Transfusion (ISBT) FUT1 gene variant database, which was similar to the weakly functional allele of FUT101W. 37(c.803G>A) as recorded by the ISBT database. The ABO genotype was heterozygous ABOA2.05/O.01.02. Combining the results of serological and genetic testing, the blood type of the blood donor was determined to be para-Bombay and A2 subtypes. Literature review has identified a pregnant women from Qingdao carrying the FUT1*01W.37 allele and 2 individuals carrying a heterozygous FUT1*01W.23 allele. This study has discovered a blood donor with coexisting para-Bombay and ABO subtype blood groups. Based on the characteristics of red blood cell surface antigens, the FUT1*01W.37 as classified as an FUT1 null allele.

Autonomy and prevention: From conflicting to complementary aims of prenatal screening.

From an ethical point of view, there is an important distinction between two types of prenatal screening. The first of these targets maternal or foetal conditions (e.g., infectious diseases, blood group sensitization) where early detection allows for interventions that improve the chances of a healthy pregnancy outcome. The second screens for foetal conditions such as Down syndrome, where a timely diagnosis in most cases only allows for a choice between preparation for a child with special needs or termination of the pregnancy. Whereas the former makes an easy fit with the prevention aim of most other population screening programmes, the latter does not. In order to steer clear from a possible eugenic reading of its aim, a wide international consensus has emerged for the view that prenatal screening of this type should have the atypical aim of helping women (couples) to make autonomous reproductive choices, rather than reducing the birth prevalence of the relevant disorders. However, keeping these types of prenatal screening apart may become increasingly difficult given the development of tests, such as the Non-Invasive Prenatal Test, which cannot only be used for both types of screening but may also lead to interconnected findings on both sides of the divide. This makes it an urgent question: What the aim or aims of this new hybrid screening should be? As neither 'prevention' nor 'autonomy' will do, we argue for a normative framework that gives both aims their due, while recognizing the tensions between them.

The relics of Jesus and Eucharistic miracles: scientific analysis of shared AB blood type.

Various relics ascribed to have been in physical contact with the historical Jesus of Nazareth have been evaluated for the presence of blood, including the Tunic of Argenteuil, the Sudarium of Oviedo, and most famously, the Shroud of Turin. Interestingly, the blood type on all three textiles was found to be AB by serological testing; a similar result was observed for various modern Eucharistic miracles, in which consecrated hosts are reported to change into human cardiac tissue and blood. As AB is a relatively rare blood type, these collective observations have been used in numerous contemporary media outlets to support the idea that all such objects share a common origin. Here, the scientific validity of mutual blood type expression is evaluated. As discussed, AB antigens are not unique to human red blood cells but are also expressed in bacteria, providing a practical connection between such varied objects. Moreover, this article clarifies that the communal presence of specific and unique polymorphic markers would be required to validate that bloodstains associated with such items truly originate from a single source.

Prevalence of ABH(O) Blood Group Secretors Among Indigenous Population of Shillong: A Cross-Sectional Study

Prevalence of ABH(O) Blood Group Secretors Among Indigenous Population of Shillong: A Cross-Sectional Study

In Situ Forming Supramolecular Nanofiber Hydrogel as a Biodegradable Liquid Embolic Agent for Postembolization Tissue Remodeling.

Embolic agents have been widely used to treat blood vessel abnormalities in interventional radiology as a minimally invasive procedure. However, only a few biodegradable liquid embolic agents exhibit high embolization performance, biodegradability, and operability. Herein, the design of in situ-forming supramolecular nanofiber (SNF) hydrogels is reported as biodegradable liquid embolic agents with the assistance of Bayesian optimization through an active learning pipeline. Chemically modified gelatin with hydrogen-bonding moieties produces fibrin-inspired nanofiber-based hydrogels with a high blood coagulation capacity. The low viscosity of the SNF hydrogels makes them injectable using a microcatheter, and the hydrogel shows sufficient tissue adhesion to the blood vessel walls and very weak adhesion to the catheter tubes. Moreover, the SNF hydrogels exhibit high blood compatibility, cytocompatibility, cell-adhesive properties, and biodegradability (in vitro and in vivo). Intravascularly delivered SNF hydrogels induce embolization of rat femoral arteries. This biodegradable liquid embolic agent could be a powerful tool for interventional radiology in the treatment of various diseases, including aortic aneurysm stent grafting, gynecological diseases, and liver cancer.

An investigation into the contributing factors to survival of ARDS patients supported by veno-venous ECMO.

This study aimed to identify characteristics associated with survival during and post Extra Corporeal Membrane Oxygenation (ECMO) therapy, in patients with acute respiratory distress syndrome (ARDS) during the COVID-19 pandemic. A retrospective observational study on 94 consecutive patients with confirmed COVID-19 induced ARDS supported by ECMO was carried out 49/94 (52.7%) patients survived to hospital discharge. Non-survivors were found to have significantly (p < .05) higher: Pre-ECMO International normalized ratios (INR), carbon dioxide partial pressure (pCO2), Acute Kidney Injury (AKI) scores and blood urea levels. Also, lower pre-ECMO peak inspiratory pressures (PIP), mean arterial pressure, saturation of arterial oxygen (SaO2), blood bicarbonate levels (HCO3), blood Ph and fewer trials off ECMO with shorter combined trial off times. Patients that did not survive were more likely to have renal impairment and have received peri-ECMO haemofiltration. Poor prognosis was significantly associated with: receiving pre-ECMO nitric oxide (HR = 3.047, CI = 1.247-7.447, p = .015), renal impairment (HR = 3.023, CI = 1.586-5.763, p < .001), AKI of 2 (HR = 3.611, CI = 1.382-9.441, p = .009) or 3 (HR = 3.275, CI = 1.235-8.685, p = .017), peri-ECMO haemofiltration (HR = 2.412, CI = 1.310-4.442, p = .005) and the ABO blood group B (HR = 3.103, CI = 1.335-7.212, p = .008). pre-ECMO high CO2 (HR = 1.134, CI = 1.031-1.248, p = .010), blood lactate (HR = 1.350, CI = 1.156-1.576, p < .001), INR (HR = 2.571, CI = 1.438-4.598, p=<0.001) and lower blood Ph (HR = 0.023, CI = 0.002-0.210, p < .001). Commonly used mortality scores may not be of use in a COVID-19 cohort of ECMO patients. The initiation of ECMO needs to be implemented prior to metabolic derangements, renal and fulminant respiratory failure.

Blood Group Detection by Using Fingerprint

Before performing blood transfusions in severe situations, blood group detection is necessary. It is done before a blood transfusion in an emergency or when checking a person's blood group for donation. Currently, lab personnel perform tests manually in the laboratory. This takes time and may result in human mistake when determining blood type. The goal of the study survey is to use image processing to reduce the amount of physical labor required to identify blood groups. The presence or absence of agglutination reaction of blood with antigen will be used to determine the blood group

A Case Report of a Suspected Neonatal Alloimmune Thrombocytopenia in a Tertiary Health Institution

Fetal and neonatal alloimmune thrombocytopenia FNAIT is the leading cause of severe thrombocytopenia in the fetus and neonate leading to serious bleeding, intracranial haemorrhage and death and also intracranial haemorrhage in full-term infants. We reported a case of a 33-year-old woman who was delivered of a male neonate via Emergency lower segment Cesarean section CS on account of premature rupture of the membrane with oligo hydramnios at an estimated gestational age EGA of 29 weeks 3 days. Her blood group is O Rhesus D positive, and Genotype HbAA. No known premorbid illness. Her last confinement was of a male child delivered via CS and died within 12 hours of birth. This reported case of suspected FNAIT was a baby received alive after CS with birthweight of 1,060g and APGAR score of 7185 transiently, and was noticed to be bleeding from the mouth and anus shortly after birth. Thereafter, he had thrombocytopenia, persistent anaemia, several apneic attacks, seizures, desaturation and died after 42 hours of life despite all prompt clinical interventions. Autopsy summary includes anaemia, central cyanosis, petechial hemorrhage, diffuse alveolar damage, cerebral edema, acute tubular necrosis, small for gestational age and placental vasculopathy. Conclusion: Fetal and neonatal alloimmune thrombocytopenia is a rare disorder and yet poses a challenging diagnosis and treatment plan in resource-limited centres especially in developing countries. Although the clinical findings of the reported suspected case were not classical as previously reported by other studies, they’re pointers to our curiosity and open to further criticism.

Fabrication of an Antibacterial/Anticoagulant Dual-Functional Surface for Left Ventricular Assist Devices via Mussel-Inspired Polydopamine Chemistry.

Infections and thrombosis remain unsolved problems for implanted cardiovascular devices, such as left ventricular assist devices. Hence, the development of surfaces with improved blood compatibility and antimicrobial properties is imperative to reduce complications after artificial heart implantation. In this work, we report a novel approach to fabricate multifunctional surfaces for left ventricular transplanted ventricular assist devices (LVADs) by immobilizing nitric oxide (NO) generation catalysts and heparin and reducing silver nanoparticles in situ. The general view, structure, and chemical compositions of the pure/modified surfaces were characterized using digital imaging, scanning electron microscope (SEM), atomic force microscope (AFM), water contact angle (WCA), X-ray photoelectron spectroscopy (XPS), and inductively coupled plasma (ICP). All of the results demonstrated that the AgNPs and heparin were successfully immobilized on the surface. The Cu ions and NO release experimental results showed that the immobilized copper ions could catalyze the production of NO from S-nitrosothiols within the biological system. Meanwhile, due to the synergistic anticoagulant effect of NO and surface-immobilized heparin, the fabricated modified surfaces exhibited antiplatelet adhesion activities and good hemocompatibility. Finally, the antimicrobial activity of the samples was evaluated by Escherichia coli and Staphylococcus aureus, and cytocompatibility was measured using human umbilical vein endothelial cells (HUVECs). The results demonstrated that silver nanoparticles (AgNPs) immobilized by surface reduction reaction did not cause any significant inhibition of cell proliferation while providing stable and effective antimicrobial properties. We envision that this simple surface modification strategy with bifunctional activities of antimicrobial and anticoagulant will find widespread use in clinically used indwelling left ventricular assist devices.

Assessment and preservation of the gene pool of the domestic &lt;i&gt;Kholmogorsk&lt;/i&gt; cattle breed in the Arkhangelsk region

The article presents the results of monitoring immunogenetic studies on the origin of Kholmogory cattle to establish their breed genetic passport for the EAB locus of blood groups. The importance of determining of the breeding animals statute taking into account the forms of the identified state of erythrocyte antigens for the purpose of registration in the federal state information and analytical system of breeding resources of the Russian Federation is noted. In breeding farms of the Arkhangelsk region over the past 29 years, the total number of animals tested for the reliability of origin was 57,311. The percentage of reliability in the monitored livestock numbers varied from 86.95% (1995-2000) to 97.28% (2016-2020). To establish the genetic passport of breeding female tribal species of the breed with up to 75% Holstein bloodline approved for breeding purposes when working with Kholmogory cattle, we conducted immunogenetic testing, which revealed 46 alleles of the EAB locus. The total frequency of the EAB locus alleles in animals of groups 1-2 with specific (typical) for the Kholmogory breed and with unique alleles was 69.38%. In group 3 of animals with alleles of the approved Holstein breed, the total frequency was set at 30.6%, in group 4 with alleles of unapproved breeds – 0.02%. It was established that the concentration of alleles G2O2, B2O2, B2G2KA’B’G’O’G’’, B2E’2G’, B2O2Y2D’, Q is very low, they are found only in some individuals. The degree of homozygosity (theoretical) is 5.6%, the number of effective alleles is 17.9.