Blanching Step Research Articles

The Effect of Washing, Blanching and Frozen Storage on Pesticide Residue in Spinach.

Spinach (Spinacia oleracea L.) is a representative green leafy vegetable commonly consumed fresh or as a ready-to-cook frozen product, with increasing consumption because of its many health-related properties. Among leafy vegetables, spinach poses a major concern in terms of pesticide residue detection due to common phytotechnical practices. In this study, spinach leaves were treated in the open field with three commercial pesticide formulations containing propamocarb, lambda-cyhalothrin, fluopicolide and chlorantraniliprole at the highest concentration. The effects of the successive processing steps of washing, blanching, freezing and frozen storage were evaluated on the levels of the four pesticide residues and the degradation product (propamocarb n-desmethyl). The washing step caused a reduction of fluopicolide and chlorantraniliprole of -47% and -43%, respectively, while having a mild effect on lambda-cyhalothrin content (+5%). A two-minute blanching step allowed for the reduction of pesticides content ranging from -41% to -4% with respect to the washed sample. Different behaviors were depicted for longer blanching times, mainly for propamocarb, reaching -56% after 10 min of treatment. Processing factors higher than 1 were reported mainly for lambda-cyhalothrin and fluopicolide. Frozen storage led to a slight increase in the pesticide content in samples treated for 6 and 10 min. The optimal blanching treatment for spinach, submitted to freezing and frozen storage, seems to be 2 min at 80 °C.

Effects of a brief blanching process on quality, safety, and shelf life of refrigerated cucumber pickles.

Refrigerated pickles are characterized by crisp, crunchy texture, opaque flesh, and fresh flavor. Typically produced without a thermal process, microbial safety relies on preventive controls, brine composition, and sufficient hold time prior to consumption. We hypothesized that brief blanching of whole cucumbers prior to pickling could provide an additional hurdle for pathogenic microbes without negatively impacting finished product quality. Blanch treatments (15, 90, or 180 s) in 80°C water were conducted in duplicate on two lots of cucumbers prior to cutting into spears, acidifying, and storing at 4°C. Enumeration of total aerobes, lactic acid bacteria, and glucose-fermenting coliforms was conducted for fresh and blanched cucumber. Texture, color, cured appearance development, and volatile compound profiles were analyzed for fresh and blanched cucumber and corresponding pickle products during refrigerated storage. The 90 s blanch consistently achieved a minimum 2-log reduction in cucumber microbiota and a predicted 5-log reduction of Escherichia coli O157:H7 up to 1.1mm into the cucumber fruit. Blanching had no impact on tissue firmness during refrigerated storage for 1 year (p>0.098). There were no differences in flavor-active lipid oxidation products (E,Z)-2,6-nonadienal and (E)-2-nonenal, and consumers (n=110) were unable to differentiate between control and 90 s blanched cucumber pickles stored for 62 days. Exocarp color and mesocarp opacity were preserved by the blanching treatment, potentially extending product shelf life. This method offers processors an option for reducing the risk of microbial contamination while maintaining the quality attributes associated with refrigerated cucumber pickles. PRACTICAL APPLICATION: Refrigerated pickles do not undergo thermal processing, which can leave them vulnerable to microbial contamination. This study illustrates that adding a brief blanching step in refrigerated pickle processing can reduce indigenous microbiota without negatively impacting quality attributes. This blanching process could assist pickled vegetable manufacturers in providing additional safeguards for consumers while maintaining a high-quality product.

Characterization of Ionic Exchange and Macroporous Resins for Their Application on the Separation and Recovery of Chlorogenic Acid from the Wastewater of Artichoke Blanching

Food wastes have traditionally been considered as dead-end materials; however, recent international, national, and regional policies strongly promote the use of these wastes as potential resources. This change of perception considers the need for sustainable exploitation of natural resources. In this context, artichoke processing produces high amounts of wastewaters, and specifically, wastewaters from the artichoke blanching step present a high content of valuable biocomponents such as carbohydrates and phenolic compounds (e.g., chlorogenic acid, 1700 ppm). In this work, the recovery of chlorogenic acid by applying sorption processes was studied. Five resins were tested, and it was found that the resin XAD 7 HP presented the best total adsorption-desorption yield (72.8%) at 20 °C. It was also found that there was a strong variation of the adsorption ratio depending on solution pH (3–7). Four models of isotherms were applied to the adsorption processes, and the Langmuir isotherm better explained the adsorption behavior. The kinetic study showed that the adsorption and desorption of chlorogenic acid followed a pseudo-second order model where the kinetic constant depended on the adsorbate concentration. In addition, it was found that the sorption process was controlled by more than just the intraparticle diffusion mechanism.

Toward the reduction of water consumption in the vegetable-processing industry through membrane technology: case study of a carrot-processing plant.

The food industry consumes large amounts of clean, potable water and in turn generates a significant amount of wastewater. In order to minimize water consumption, membrane technologies represent a suitable solution for the treatment of wastewater before it is recycled as process water. Many studies have shown the effectiveness of this technology in the dairy industry, but there are few studies in the fruit- and vegetable-processing sectors. A recently developed methodology for the reduction of water consumption was tested here. Compounds to be eliminated were identified through chemical analysis of several wastewater samples from a carrot-peeling process. Drinking-water quality was selected as our target. Total suspended solids (TSS), fructose, glucose and sucrose were identified as key parameters. Salts (particularly Ca2+ and Mg2+), pH and carbonate hardness (CH) were identified as indicators for evaluating the risk of scaling and corrosion. Based on these results, sieving followed by a 0.5-μm microfiltration (MF) was chosen as the process for pre-treatment. Four nanofiltration (NF) membranes (NFW from SYNDER, DK from GE, NF270 from DOW and SR3D from KOCH) and three reverse osmosis (RO) membranes (ESPA4 from Nitto Group Company, BW30 from DOW and HRX from KOCH) were then tested for the capacity to minimize chemical oxygen demand (COD) and to principally remove sugars. These membranes were then evaluated in terms of permeability and rejection rates. High-quality water could be obtained with RO membranes at low pressure (up to 15bar) while limiting fouling risks. Rejection rates up to 98.3, 98.0, 99.2, 99.2 and 99.4% for conductivity, COD, fructose, glucose and sucrose, respectively, were achieved. These results are very encouraging for future reuse in vegetable processing before the blanching step, after an additional disinfection treatment.

Blanching impact on pigments, glucosinolates, and phenolics of dehydrated broccoli by-products

Because of high water content, the valorisation of broccoli by-products requires dehydration that can preserve bioactive compounds. Blanching pre-treatment has been reported to improve the drying rate of broccoli. As a thermal treatment, it promotes also enzyme inactivation. Therefore, in this study, the impact of pre-dehydration blanching step, freeze-drying, air-drying at 40 °C, and microwave hydrodiffusion and gravity (MHG) dehydration on the levels of pigments, glucosinolates, and phenolics, was evaluated by UHPLC-DAD-ESI/MSn. When compared to freeze-drying, a technique known to preserve compounds, a pre-blanching step increased the extractability of both pigments and phenolics, while air-drying only retained 49% of the pigments and 70% of phenolics, both without affecting glucosinolates. However, when air-drying was preceded by blanching, less than 50% of compounds were retained. On the other hand, MHG dehydration increased the phenolics extractability by 26%, particularly that of kaempferol derivatives while also retaining the amount of the glucosinolate glucoraphanin, when compared to freeze-drying. Nevertheless, only 23% of indole glucosinolates were recovered and pigments were severely reduced, with lutein accounting only for 32% and only chlorophyll b was observed in trace amounts after MHG dehydration. Therefore, to valorise broccoli by-products as ingredients, different drying technologies may be used when targeting different composition richness: freeze-drying is suitable for pigments and glucosinolates, air-drying is suitable for glucosinolates, while MHG promotes the extractability of phenolic compounds.

Potato (Solanum tuberosum L.) nutritional changes associated with French fry processing: Comparison of low-temperature long-time and high-temperature short-time blanching and frying treatments

Six potato cultivars (Fianna, Innovator, Mondial, Navigator, Panamera and Savanna) were evaluated for moisture, colour, oil, protein and mineral element changes associated with French fry processing. Processing involved blanching French fry strips at 75 °C for 10 min (low-temperature long-time [LTLT] blanching) or 85 °C for 5 min (high-temperature short-time [HTST] blanching), before frying them at 160 °C for 2 min (LTLT frying) or 180 °C for 1 min (HTST frying). The results revealed that genotype and the blanching and frying treatments significantly affected the nutritional value of potatoes. Savanna showed the smallest changes, which generally did not differ among treatments, while nutritional changes varied widely in the other cultivars. Cultivars exhibited a reduction in moisture (30–60%), lightness (≤40%), Fe and Al (<150%). However, an increase in oil (8000–19307%), protein (≤110%) and mineral elements, such as Ca, Zn, Cu, P, K, Mg, Na and Mn (<150%), was detected. This increase was mainly attributed to moisture loss associated with the blanching step. HTST blanching resulted in the highest oil absorption in Fianna, Innovator and Panamera, compared to LTLT blanching. These results indicate that the nutritional value of French fries mainly depends on cultivar and the blanching parameters used.

Temperature impacts the sporulation capacities and spore resistance of Moorella thermoacetica

Temperatures encountered in cannery allow growth of thermophilic spore-forming bacteria, including the strictly anaerobe Moorella thermoacetica, which grows optimally from 55 °C to 65 °C and is the main cause of spoilage of low-acid canned foods (LACFs) at high temperature. Resistance to wet-heat, biocides and UV-C of spores formed at different temperatures was assessed either for a selection of M. thermoacetica strains or for the strain M. thermoacetica ATCC 39073. Spores formed at 45 °C were significantly more sensitive to wet-heat than spores produced at 55 °C, while spores produced at 65 °C were as heat-resistant as spores produced at 55 °C. Spores of M. thermoacetica ATCC 39073 produced at 45 °C were significantly less resistant to peracetic acid than spores formed at 55 °C, while no difference in sensitivity to H2O2 or to UV-C treatment was observed whatever the sporulation temperature. However, both types of treatment enabled at least a 3.3 log CFU/mL reduction of M. thermoacetica ATCC 39073 spores. M. thermoacetica spores thus showed higher resistance properties when sporulation temperature was close to optimal growth temperature. These findings suggest food spoilage due to M. thermoacetica species could be controllable by holding temperatures below optimal growth temperature from the blanching step to the can filling step.

Microbiological Load of Edible Insects Found in Belgium.

Edible insects are gaining more and more attention as a sustainable source of animal protein for food and feed in the future. In Belgium, some insect products can be found on the market, and consumers are sourcing fresh insects from fishing stores or towards traditional markets to find exotic insects that are illegal and not sanitarily controlled. From this perspective, this study aims to characterize the microbial load of edible insects found in Belgium (i.e., fresh mealworms and house crickets from European farms and smoked termites and caterpillars from a traditional Congolese market) and to evaluate the efficiency of different processing methods (blanching for all species and freeze-drying and sterilization for European species) in reducing microorganism counts. All untreated insect samples had a total aerobic count higher than the limit for fresh minced meat (6.7 log cfu/g). Nevertheless, a species-dependent blanching step has led to a reduction of the total aerobic count under this limit, except for one caterpillar species. Freeze-drying and sterilization treatments on European species were also effective in reducing the total aerobic count. Yeast and mold counts for untreated insects were above the Good Manufacturing Practice limits for raw meat, but all treatments attained a reduction of these microorganisms under this limit. These results confirmed that fresh insects, but also smoked insects from non-European trades, need a cooking step (at least composed of a first blanching step) before consumption. Therefore, blanching timing for each studied insect species is proposed and discussed.

Optimization of an incubation step to maximize sulforaphane content in pre-processed broccoli.

Sulforaphane is a powerful anticancer compound, found naturally in food, which comes from the hydrolysis of glucoraphanin, the main glucosinolate of broccoli. The aim of this work was to maximize sulforaphane content in broccoli by designing an incubation step after subjecting broccoli pieces to an optimized blanching step. Incubation was optimized through a Box-Behnken design using ascorbic acid concentration, incubation temperature and incubation time as factors. The optimal incubation conditions were 38°C for 3h and 0.22mg ascorbic acid per g fresh broccoli. The maximum sulforaphane concentration predicted by the model was 8.0µmolg-1, which was confirmed experimentally yielding a value of 8.1±0.3µmolg-1. This represents a 585% increase with respect to fresh broccoli and a 119% increase in relation to blanched broccoli, equivalent to a conversion of 94% of glucoraphanin. The process proposed here allows maximizing sulforaphane content, thus avoiding artificial chemical synthesis. The compound could probably be isolated from broccoli, and may find application as nutraceutical or functional ingredient.

Purification of artichoke polyphenols by using membrane filtration and polymeric resins

The present study aimed at evaluating the potential of an integrated process based on the use of membrane technology and adsorbent resins for the recovery, concentration and purification of phenolic compounds from artichoke wastewaters.In particular, artichoke wastewaters coming from the blanching step were pre-treated by ultrafiltration (UF) in order to remove suspended solids and macromolecular compounds. The UF permeate was submitted to a nanofiltration (NF) process producing a concentrated fraction enriched in phenolic and sugar compounds.Three different macroporous resins were tested through adsorption/desorption methods to produce purified phenolic fractions with high antioxidant activity. Samples produced in UF, NF and adsorption–desorption tests were assayed for phenolic composition (chlorogenic acid and apigenin 7-O-glucoside), sugar composition (fructose, glucose and sucrose) and antioxidant activity.Among the three different tested resins, the S 7968 offered the best performance in terms of adsorption/desorption ratio for chlorogenic acid, with a total adsorption/desorption yield (TADY) of 63.39%; for the apigenin 7-O-glucoside the S 7968 and the S 2328 resins showed a TADY in the range 68.31–78.45%.

Quantitative assessment of the risk of microbial spoilage in foods. Prediction of non-stability at 55 °C caused by Geobacillus stearothermophilus in canned green beans

Microbial spoilage of canned foods by thermophilic and highly heat-resistant spore-forming bacteria, such as Geobacillus stearothermophilus, is a persistent problem in the food industry. An incubation test at 55°C for 7days, then validation of biological stability, is used as an indicator of compliance with good manufacturing practices. We propose a microbial risk assessment model predicting the percentage of non-stability due to G. stearothermophilus in canned green beans manufactured by a French company. The model accounts for initial microbial contaminations of fresh unprocessed green beans with G. stearothermophilus, cross-contaminations in the processing chain, inactivation processes and probability of survival and growth. The sterilization process is modeled by an equivalent heating time depending on sterilization value F0 and on G. stearothermophilus resistance parameter zT. Following the recommendations of international organizations, second order Monte-Carlo simulations are used, separately propagating uncertainty and variability on parameters.As a result of the model, the mean predicted non-stability rate is of 0.5%, with a 95% uncertainty interval of [0.1%; 1.2%], which is highly similar to data communicated by the French industry. A sensitivity analysis based on Sobol indices and some scenario tests underline the importance of cross-contamination at the blanching step, in addition to inactivation due to the sterilization process.

Optimization of a blanching step to maximize sulforaphane synthesis in broccoli florets

A blanching step was designed to favor sulforaphane synthesis in broccoli. Blanching was optimised through a central composite design, and the effects of temperature (50–70°C) and immersion time in water (5–15min) on the content of total glucosinolates, glucoraphanin, sulforaphane, and myrosinase activity were determined. Results were analysed by ANOVA and the optimal condition was determined through response surface methodology. Temperature between 50 and 60°C significantly increased sulforaphane content (p<0.05), whilst blanching at 70 and 74°C diminished significantly this content, compared to fresh broccoli. The optimal blanching conditions given by the statistical model were immersion in water at 57°C for 13min; coinciding with the minimum glucosinolates and glucoraphanin content, and with the maximum myrosinase activity. In the optimal conditions, the predicted response of 4.0μmol sulforaphane/g dry matter was confirmed experimentally. This value represents a 237% increase with respect to the fresh vegetable.

Effects of blanching pre-treatment and sugar composition of the osmotic solution on physico-chemical, morphological and antioxidant characteristics of osmodehydrated blueberries (Vaccinium corymbosum L.)

The effect of a steam blanching preconditioning step was investigated on the chemical, nutraceutical and morphological characteristics of osmo-dehydrated blueberries (Vaccinium corymbosum L.) in sucrose or glucose/fructose osmotic solutions. Mass transfer phenomena (solid gain, water loss and sugar gain) were monitored into the samples at intervals over a 24-h period of osmodehydration. The antioxidant capacity of untreated and osmo-dehydrated fruits was evaluated by total phenolics, total and individual anthocyanins and antioxidant activity (amperometric method), while colorimetric (luminance) and morphological (area, shape factor, fractal dimension) characteristics of the berries were determined by image-analysis technique. Results showed that the blanching step increased mass transfer phenomena in the course of the osmodehydration treatments (approx +55% solid gain and +33% water loss) and reduced the loss of phenolic compounds, improving the retention of antioxidant capacity in the final product (from approx 66.9–56.9% in not blanched berries to 88.6–95.7% in blanched ones). Blanched berries had also deeper colour and smoother surface. Slight differences in mass balances and in the final composition of berries could be ascribed to the kind of osmotic solution used.

Optimization of a process to obtain selenium-enriched freeze-dried broccoli with high antioxidant properties

Broccoli exhibits a high content of antioxidant and anticarcinogenic compounds, such as polyphenols and selenium. Since it is mostly consumed as a processed food, elucidating the processing conditions that minimize nutritional losses becomes relevant. The process composed of a blanching step followed by freeze-drying of selenium-enriched broccoli was optimized, in order to maximize the content of Se-methyl selenocysteine (SMSeC), total polyphenols content and anti-radical power (ARP). The optimal blanching conditions agreed for the three responses, and were immersion in water at 72 °C during 1 min. The optimal freeze-drying conditions were slow freezing rate, atmospheric pressure (96.13 kPa), and application of infrared radiation (IR). These conditions allowed keeping 25% ARP, 18% polyphenols content and 174% SMSeC compared to the fresh vegetable. These results will help in establishing the most adequate processing conditions that allow keeping the functional properties of broccoli as a processed food.

Quality Comparison of Continuous Steam Sterilization Segmented‐Flow Aseptic Processing versus Conventional Canning of Whole and Sliced Mushrooms

This study was carried out to investigate segmented-flow aseptic processing of particle foods. A pilot-scale continuous steam sterilization unit capable of producing shelf stable aseptically processed whole and sliced mushrooms was developed. The system utilized pressurized steam as the heating medium to achieve high temperature-short time processing conditions with high and uniform heat transfer that will enable static temperature penetration studies for process development. Segmented-flow technology produced a narrower residence time distribution than pipe-flow aseptic processing; thus, whole and sliced mushrooms were processed only as long as needed to achieve the target F₀ = 7.0 min and were not overcooked. Continuous steam sterilization segmented-flow aseptic processing produced shelf stable aseptically processed mushrooms of superior quality to conventionally canned mushrooms. When compared to conventionally canned mushrooms, aseptically processed yield (weight basis) increased 6.1% (SD = 2.9%) and 6.6% (SD = 2.2%), whiteness (L) improved 3.1% (SD = 1.9%) and 4.7% (SD = 0.7%), color difference (ΔE) improved 6.0% (SD = 1.3%) and 8.5% (SD = 1.5%), and texture improved 3.9% (SD = 1.7%) and 4.6% (SD = 4.2%), for whole and sliced mushrooms, respectively. Segmented-flow aseptic processing eliminated a separate blanching step, eliminated the unnecessary packaging of water and promoted the use of bag-in-box and other versatile aseptic packaging methods. Segmented-flow aseptic processing is capable of producing shelf stable aseptically processed particle foods of superior quality to a conventionally canned product. This unique continuous steam sterilization process eliminates the need for a separate blanching step, reduces or eliminates the need for a liquid carrier, and promotes the use of bag-in-box and other versatile aseptic packaging methods.

Traditional and industrial oven-dry processing of olive fruits: influence on textural properties, cell wall polysaccharide composition, and enzymatic activity

The preparation of table olives according to the Italian traditional “Ferrandina” method (Fer) includes an initial blanching step of black Cassanese olives, followed by salting and oven-drying. Its industrial implementation, also called the “Sybaris” method (Syb), replaces the blanching procedure by cutting the olives followed by immersion in water. The measurement of tensile properties showed that the Fer processing increased the weakness, softness, and deformability of the skin and the flesh of olive fruits, while the flesh of the Syb fruits became stronger and stiffer. These differences are probably correlated to the degradation and/or reorganisation of cell wall polysaccharides in the fruits. The degradation of pectic and hemicellulosic polysaccharides in the Fer olives was inferred by their increased solubility in aqueous solutions. Contrarily, retention of pectic polysaccharides was observed in Syb olives. As no correlation was found between cell wall degrading enzymatic activities and cell wall polysaccharides extractability, it is probable that these modifications were driven by heat.

Steam-Blanched Highbush Blueberry (Vaccinium corymbosum L.) Juice: Phenolic Profile and Antioxidant Capacity in Relation to Cultivar Selection

High-quality standards in blueberry juice can be obtained only taking into account fruit compositional variability and its preservation along the processing chain. In this work, five highbush blueberry cultivars from the same environmental growing conditions were individually processed into juice after an initial blanching step and the influence was studied of the cultivar on juice phenolic content, distribution and relative antioxidant activity, measured as scavenging capacity on the artificial free-radical 2,2-diphenyl-1-picrylhydrazyl (DPPH*). A chromatographic protocol was developed to separate all main phenolic compounds in berries. A total of 15 glycosylated anthocyanins, catechin, galactoside, glucoside, and rhamnoside quercetin 3-derivatives, and main benzoic and cinnamic acids were identified. The total content and relative distribution in anthocyanins, chlorogenic acid, and quercetin of each juice were dependent upon cultivar, and the total content was highly correlated (rxy=0.97) to the antioxidant capacity. A selective protective effect of berry blanching in juice processing can be observed on more labile anthocyanin compounds.

The Influence of Processing and Preservation on the Retention of Health‐Promoting Compounds in Broccoli

In this work the effects of common household practices such as chilling, freezing, and cooking on vitamin C retention in broccoli (Marathon cv.), as well as their influence on the release of sulforaphane upon enzymatic hydrolysis of glucoraphanin by the endogenous enzyme myrosinase, were investigated. When chilled at 6 degrees C and 95% R.H. for 35 d, broccoli showed a vitamin C and sulforaphane loss of about 39% and 29%, respectively, while storage at -18 degrees C for 60 d resulted in similar losses, but mainly due to the blanching step. Boiling, steaming, microwaving, pressure-cooking, and the combined use of pressure and microwaves were the cooking methods investigated. Boiling and steaming caused significant vitamin C losses, 34% and 22%, respectively, while with the other treatments more than 90% retention was observed. Sulforaphane was no more detectable after boiling or steaming, while pressure/microwave cooking did not cause any significant loss. The quantitative distribution of these moieties in the florets and stems of fresh and chilled broccoli was also investigated.

Application of SAFES (systematic approach to food engineering systems) methodology to French fries manufacture

Because of the high quality demanded by consumers, the study of French fries manufacture considering the product as an homogeneous system does not provide enough information on main compositional and volumetric changes occurring during potato processing. Attending to recent food quality and safety requirements, the SAFES methodology has been developed to precisely describe and quantify changes taking place throughout the operations involved in food processing. Considering different phases, components and aggregation states present in the food and applying the knowledge about its structure and the interactions between its components, those properties directly connected to food quality and safety can be defined. In the present study, the application of SAFES methodology to French fries manufacture has been proved to be a useful tool to describe some textural attributes from quantitative changes in water and oil content, as well as in volume and starch aggregation state. Studying the French fries manufacture process by applying the SAFES methodology also showed the need of additional information in certain areas of knowledge, as it could be the inactivation rate of enzymes that catalyse oxidation and the extraction rate of reducing carbohydrates during the blanching step. Apart from the experimental data and the mathematical models found in the bibliography, several hypotheses were formulated in relation to the amount of water retained by starch granules after the blanching process and the crust formation and development during the deep frying step. Information on water vapour retention by the crust layer at the end of the deep frying process was also considered, as it is directly connected with French fries quality after cooling.

Solubilization of carotenoids from carrot juice and spinach in lipid phases: II. Modeling the duodenal environment.

We have been investigating the factors determining the bioavailability of carotenoids from vegetables. The previous paper [Rich, G.T., Bailey, A.L., Faulks, R.M., Parker, M.L., Wickham, M.S.J., and Fillery-Travis, A. (2003) Solubilization of Carotenoids from Carrot Juice and Spinach in Lipid Phases: I. Modeling the Gastric Lumen, Lipids 38, 933-945] modeled the gastric lumen and studied the solubilization pathway of carotenes and lutein from carrot juice and homogenized spinach to oil. Using the same vegetable preparations, we have extended our investigations to solubilization pathways potentially available in the duodenum and looked at the ease of solubilization of carotenes and lutein within simplified lipid micellar and oil phases present within the duodenum during digestion. Micellar solubility of raw spinach carotenoids was low and was enhanced by freezing, which involved a blanching step. The efficiency of solubilization of carotenoids in glycodeoxycholate micelles decreased in the order lutein(carrot) > lutein(blanched-frozen spinach) > carotene(blanched-frozen spinach) > carotene(carrot). Frozen spinach carotenoids were less soluble in simple micelles of taurocholate than of glycodeoxycholate. The results comparing the solubility of the carotenoids in mixed micelles (bile salt with lecithin) with simple bile salt micelles are explained by the relative stability of the carotenoid in the organelle compared to that in the micelle. The latter is largely determined by the polarity of the micelle. Below their critical micelle concentration (CMC), bile salts inhibit transfer of carotenoids from tissue to a lipid oil phase. Above their CMC, the bile salts that solubilize a carotenoid can provide an additional route to the oil from the tissue for that carotenoid by virtue of the equilibrium between micellar phases and the interfacial pathway. Mixed micellar phases inhibit transfer of both carotenoids from the tissue to the oil phase, thereby minimizing this futile pathway.