Blade Boundary Research Articles

Genome-wide analysis of spatiotemporal expression patterns during rice leaf development

BackgroundRice leaves consist of three distinct regions along a proximal-distal axis, namely the leaf blade, sheath, and blade-sheath boundary region. Each region has a unique morphology and function, but the genetic programs underlying the development of each region are poorly understood. To fully elucidate rice leaf development and discover genes with unique functions in rice and grasses, it is crucial to explore genome-wide transcriptional profiles during the development of the three regions.ResultsIn this study, we performed microarray analysis to profile the spatial and temporal patterns of gene expression in the rice leaf using dissected parts of leaves sampled in broad developmental stages. The dynamics in each region revealed that the transcriptomes changed dramatically throughout the progress of tissue differentiation, and those of the leaf blade and sheath differed greatly at the mature stage. Cluster analysis of expression patterns among leaf parts revealed groups of genes that may be involved in specific biological processes related to rice leaf development. Moreover, we found novel genes potentially involved in rice leaf development using a combination of transcriptome data and in situ hybridization, and analyzed their spatial expression patterns at high resolution. We successfully identified multiple genes that exhibit localized expression in tissues characteristic of rice or grass leaves.ConclusionsAlthough the genetic mechanisms of leaf development have been elucidated in several eudicots, direct application of that information to rice and grasses is not appropriate due to the morphological and developmental differences between them. Our analysis provides not only insights into the development of rice leaves but also expression profiles that serve as a valuable resource for gene discovery. The genes and gene clusters identified in this study may facilitate future research on the unique developmental mechanisms of rice leaves.

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement

High throughput phenotyping (phenomics) is a powerful tool for linking genes to their functions (see review and recent examples). Leaves are the primary photosynthetic organ, and their size and shape vary developmentally and environmentally within a plant. For these reasons studies on leaf morphology require measurement of multiple parameters from numerous leaves, which is best done by semi-automated phenomics tools. Canopy shade is an important environmental cue that affects plant architecture and life history; the suite of responses is collectively called the shade avoidance syndrome (SAS). Among SAS responses, shade induced leaf petiole elongation and changes in blade area are particularly useful as indices. To date, leaf shape programs (e.g. SHAPE, LAMINA, LeafAnalyzer, LEAFPROCESSOR) can measure leaf outlines and categorize leaf shapes, but can not output petiole length. Lack of large-scale measurement systems of leaf petioles has inhibited phenomics approaches to SAS research. In this paper, we describe a newly developed ImageJ plugin, called LeafJ, which can rapidly measure petiole length and leaf blade parameters of the model plant Arabidopsis thaliana. For the occasional leaf that required manual correction of the petiole/leaf blade boundary we used a touch-screen tablet. Further, leaf cell shape and leaf cell numbers are important determinants of leaf size. Separate from LeafJ we also present a protocol for using a touch-screen tablet for measuring cell shape, area, and size. Our leaf trait measurement system is not limited to shade-avoidance research and will accelerate leaf phenotyping of many mutants and screening plants by leaf phenotyping.

LeafJ: An ImageJ Plugin for Semi-automated Leaf Shape Measurement

High throughput phenotyping (phenomics) is a powerful tool for linking genes to their functions (see review1 and recent examples2-4). Leaves are the primary photosynthetic organ, and their size and shape vary developmentally and environmentally within a plant. For these reasons studies on leaf morphology require measurement of multiple parameters from numerous leaves, which is best done by semi-automated phenomics tools5,6. Canopy shade is an important environmental cue that affects plant architecture and life history; the suite of responses is collectively called the shade avoidance syndrome (SAS)7. Among SAS responses, shade induced leaf petiole elongation and changes in blade area are particularly useful as indices8. To date, leaf shape programs (e.g. SHAPE9, LAMINA10, LeafAnalyzer11, LEAFPROCESSOR12) can measure leaf outlines and categorize leaf shapes, but can not output petiole length. Lack of large-scale measurement systems of leaf petioles has inhibited phenomics approaches to SAS research. In this paper, we describe a newly developed ImageJ plugin, called LeafJ, which can rapidly measure petiole length and leaf blade parameters of the model plant Arabidopsis thaliana. For the occasional leaf that required manual correction of the petiole/leaf blade boundary we used a touch-screen tablet. Further, leaf cell shape and leaf cell numbers are important determinants of leaf size13. Separate from LeafJ we also present a protocol for using a touch-screen tablet for measuring cell shape, area, and size. Our leaf trait measurement system is not limited to shade-avoidance research and will accelerate leaf phenotyping of many mutants and screening plants by leaf phenotyping.

Interactive coupling between a tree and raindrops

ABSTRACTThis paper presents a novel approach for simulating the dynamic coupling between a tree and raindrops based on physical deformation and fluid simulation. By the approach, tree animation in the rain can be simulated in a two‐resolution way: branch motion and leaf motion. The branch is represented by the Euler–Bernoulli beam model, and the leaf petiole is represented by the three‐prism elastic model. Interaction coupling liquid motion on the hydrophilic surface with a flexible petiole is well implemented by a special design. To simplify the computation process, instead of the computation‐intensive three‐dimensional Navier–Stokes equations, shallow water equations are used to simulate the water dynamics together with the whole leaf deformation. Simulation has been also made to various phenomena incurred from the interactive coupling. These include, among others, part of impacting raindrops splashing into the air with the remaining flowing along the slant of the leaf and merging into larger ones or hanging on the blade boundary, with the leaf rebounding and vibrating after the drops fall off the leaf. A level‐of‐detail approach is exploited to accelerate rendering in views of different distances. The experimental results illustrate that the approach can be applied to efficiently generate realistic details of the interactive coupling between a tree and raindrops. Copyright © 2012 John Wiley & Sons, Ltd.

Advanced Nonaxisymmetric Endwall Contouring for Axial Compressors by Generating an Aerodynamic Separator—Part II: Experimental and Numerical Cascade Investigation

Modern methods for axial compressor design are capable of shaping the blade surfaces in a three-dimensional way. Linking these methods with automated optimization techniques provides a major benefit to the design process. The application of nonaxisymmetric contoured endwalls is considered to be very successful in turbine rotors and vanes. Concerning axial compressors, nonaxisymmetric endwalls are still a field of research. This two-part paper presents the recent development of a novel endwall design. A vortex created by a nonaxisymmetric endwall groove acts as an aerodynamic separator, preventing the passage vortex from interacting with the suction side boundary layer. This major impact on the secondary flow results in a significant loss reduction by means of load redistribution, reduction in recirculation areas, and suppressed corner separation. Part I of this paper deals with the endwall design and its compressor application. The resulting flow phenomena and physics are described and analyzed in detail. The second paper presents the detailed experimental and numerical investigation of the developed endwall groove. The measurements carried out at the transonic cascade wind tunnel of DLR in Cologne, demonstrated a considerable influence on the cascade performance. A loss reduction and redistribution of the cascade loading were achieved at the aerodynamic design point, as well as near the stall condition of the cascade. This behavior is well predicted by the numerical simulation. The combined analysis of experimental and numerical flow patterns allows a detailed interpretation and description of the resulting flow phenomena. In this context, high fidelity 3D-Reynolds-averaged Navier–Stokes flow simulations are required to analyze the complex blade and endwall boundary layer interaction.

Automated visual inspection for microdrills in printed circuit board production

The rapid growth in printed circuit board production has led to the growing importance of microdrilling. Owing to the trend for high-density circuits on printed circuit boards, the diameter of a microdrill has to be much finer than ever before. The inspection for microdrills becomes more difficult and the detection of defects is harder. An automated visual inspection scheme for detecting the major defects of microdrills is proposed. The colour images of microdrills are first derived, and the boundary of the lip relief plane is extracted. A robust K-curvature corner detection algorithm is then used to detect the corners on the blade boundaries and to split boundaries into segments. The least-squares linear regression method is used to fit the segments into linear equations. With the fitted equations, the intersections on the boundaries of two facets are located and their distances (gap or overlap) are measured. In addition, the defects of taper and flare are identified by comparing the angle measurements with predefined specifications. Two types of microdrills are used to verify the proposed inspection process. Experimental results show that the proposed scheme reliably achieves precise inspection.

Periodic Transition on an Axial Compressor Stator: Incidence and Clocking Effects: Part II—Transition Onset Predictions

Transition onset observations from a 1.5-stage axial compressor outlet stator presented in Part I of this paper are compared with the predictions of conventional transition correlations applied in a quasi-steady manner. The viscous/inviscid interaction code MISES is used to predict the blade surface pressure distributions and boundary layer development. The temporal variation in transition onset is then predicted using ensemble-averaged free-stream turbulence data from the compressor measurements. This simple procedure captures most significant features of the complex transition process on the compressor, and is clearly superior to fixed transition models based on long-term average free-stream turbulence levels. Parallel computations for both natural and bypass transition modes indicate that the natural transition mode tends to dominate on the compressor. This is at variance with turbine airfoil experience, where bypass transition is clearly more important. Comparison of prediction and experiment highlights the significance of leading edge potential flow interactions in promoting periodic wake-induced transition. Viscous/inviscid interactions in the neighborhood of transition can also have an important influence on boundary layer stability and separation phenomena.

Computational Modelling of a Transitional 3D Turbine-cascade Flow using a Modified Low-Re k — ϵ Model and a Multi-block Scheme

A computational finite-volume scheme, based on the structured-grid multi-block approach and incorporating a new variant from the group of low-Reynolds-number k — ϵ models, is applied to the transitional flow in a 3D turbine cascade. The turbulence model is designed, by reference to a particular one-equation model, to give the correct variation of turbulence length scale as the wall is approached. Its performance characteristics are first demonstrated through calculations for a transitional flat-plate boundary layer, prior to its application to the 3D turbine cascade. The characteristics of the cascade flow are examined in detail, especially in terms of the structure of the passage vortex and losses arising from turbulence. The latter are shown to be over-estimated relative to the experimental levels due to a premature onset of transition in the blade's boundary layers, especially near the end wall.

Prediction of Cascade Performance Using an Incompressible Navier–Stokes Technique

A fully elliptic, control volume solution of the two-dimensional incompressible Navier–Stokes equations for the prediction of cascade performance over a wide range is presented in this paper. The numerical technique is based on a new pressure substitution method. A Poisson equation is derived from the pressure-weighted substitution of the full momentum equations into the continuity equation. The analysis of a double circular arc compressor cascade is presented, and the results are compared with the available experimental data at various incidence angles. Good agreement is obtained for the blade pressure distribution, boundary layer and wake profiles, skin friction coefficient, losses and outlet angles. Turbulence effects are simulated by the low-Reynolds-number version of the k–ε turbulence model.

The Effect of Irreversibility on the Capacity of a Turbine Blade Row

If the assumption is made that the process followed by the expansion of a gas in a converging blade row is isentropic, thermodynamic analysis leads to the result that the attainment of maximum mass flow, for a given flow area and predetermined inlet pressure and temperature, occurs when sonic velocity is reached at the throat. If, however, as in practice, the expansion proceeds with internal frictional losses, and if certain common assumptions are made regarding these losses, then, assuming adiabatic flow, thermodynamic analysis shows that the maximum flow will occur at a Mach number less than unity. If this paradox is not noticed, it may lead to an appreciable underestimate of the mass flow passed by a blade row for a given set of inlet conditions. This paper examines the relationship between blade row losses and boundary layer characteristics in an attempt to rationalize the calculation of the capacity of a turbine as modified by irreversibility.

A Theory of the Direct and Inverse Problems of Compressible Flow Past Cascade of Arbitrary Airfoils

A Theory of the Direct and Inverse Problems of Compressible Flow Past Cascade of Arbitrary Airfoils