Blackberry Seeds Research Articles

Assessment of the Nutritional Benefits and Aflatoxin B1 Adsorption Properties of Blackberry Seed Cold-Pressed Oil By-Product.

This study explores the potential valorization of blackberry seed oil cake (BBSOC), a by-product of cold-pressed blackberry seed oil (Rubus fruticosus L.), as a nutritionally valuable material with aflatoxin B1 (AFB1) adsorption properties. The chemical and mineral composition, polyphenols, and antioxidant activity of BBSOC flour were assessed. BBSOC was found to be a significant source of fiber (62.09% dry weight) and essential minerals such as Fe (123.48 mg/kg), Mg (1281.40 mg/kg), K (3087.61 mg/kg), and Ca (1568.41 mg/kg). The high polyphenol content, especially ellagic acid, highlighted its biologically active potential. Moreover, BBSOC demonstrated effective biosorption of AFB1 under in vitro conditions at 37 °C, with adsorption efficiencies of 85.36% and 87.01% at pH 3 and 7, respectively. Characterization techniques including SEM, FTIR analysis, Boehm titration, and pH zero charge determination confirmed its AFB1 adsorbing properties. This valorization process reintroduces a secondary product into the food chain, supporting the circular economy and zero-waste concepts. Thus, BBSOC is nutritionally rich and effective in AFB1 biosorption, presenting potential applications as a food or feed additive.

Optimization and component identification of ultrasound-assisted extraction of functional compounds from waste blackberry (Rubus fruticosus Pollich) seeds.

Blackberry seeds, as a by-product of processing, have potential bioactive substances and activities. A response surface method was used to determine the optimal conditions of blackberry seed extracts (BSEs) with high 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity by ultrasound-assisted extraction (UAE). The composition and antioxidant capacity of BSEs were further analyzed. The optimal conditions were material-to-liquid ratio of 0.07 g mL-1, ethanol concentration of 56%, extraction temperature of 39 °C and ultrasonic power of 260 W. Using these conditions, the extraction yield and total polysaccharide, phenolic and anthocyanin contents in BSEs were 0.062 g g-1 and 633.91, 36.21 and 3.07 mg g-1, respectively. The Fourier transform infrared spectra of BSEs exhibited characteristic peaks associated with polysaccharide absorption. The antioxidant capacity, DPPH and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity, and ferric reducing antioxidant power of BSEs were 1533.19, 1021.93 and 1093.38 mmol Trolox equivalent g-1, respectively. The delphinidin-3-O-glucoside, paeoniflorin-3-O-glucoside and cyanidin-3-O-arabinoside contents in BSEs were 3.05,12.76 and 1895.90 ± 3.45 μg g-1. Five polyphenols including gallic acid, coumaric acid, ferulic acid, catechin and caffeic acid were identified and quantified in BSEs with its contents at 8850.43, 5053.26, 4984.65, 1846.91 and 192.40 μg g-1. These results provide a method for preparing BSE containing functional components such as polysaccharides, phenols and anthocyanins through UAE, and BSEs have potential application in food industries. © 2024 Society of Chemical Industry.

Blackberry synthetic seeds storage: effects of temperature, time, and sowing substrate

In vitro propagation, is becoming the predominant method for blackberry propagation due to its advantages compared to agamic traditional propagation methods. Synthetic seed technology represents a promising approach to further enhance the productivity of in vitro propagation facilitating the exchange of plant materials among laboratories and contributing to germplasm conservation efforts. This study aimed to establish an optimal protocol for the storage and sowing of synthetic blackberry seeds obtained through the encapsulation of clump bases. The synthetic seeds were sown without storage (Control) and after storage periods of 30, 60, and 120 days at 4 °C and 25 °C in the dark, in three different substrates (agarised, perlite, and potting). After forty-five days from sowing viability, regeneration rate, shoot and root numbers and lengths, as well as fresh and dry weights of the plantlets, were assessed. Results indicated that agarised substrate consistently exhibited favourable outcomes, with sustained regeneration rates and robust plantlet development even after prolonged storage at 4 °C. Synthetic seeds sown in perlite and potting substrates demonstrated enhanced regeneration rates following storage at 4 °C for 60 and 120 days. On the contrary, storage at 25 °C resulted in a notable decline in regeneration rate, highlighting its inadequacy for blackberry synthetic seed conservation purposes. These findings underscore the importance of sowing substrate selection and storage temperature in optimizing the storage and sowing protocols for synthetic blackberry seeds.

COMPARATIVE STUDY OF FATTY ACIDS FORMATION IN NATURAL AND GENETICALLY MODIFIED BLACKBERRY SEEDS

The work is devoted to a comparative study of fatty acids formation in natural and genetically modified blackberry seeds (collected in the foothill landscapes of Alaverdi Town, Republic of Armenia, and those of genetically modified blackberries (Rubus caesius L.), grown in greenhouse conditions in Yerevan City, harvest of 2023) capable of accumulating them in large quantities in order to justify the choice of the most valuable oilseed raw material for the production of natural oils that are in high demand in the food industry and cosmetology. It has been shown that the oil content of the studied genetically modified blackberry seeds is about two times higher than that of natural blackberry seeds. These seeds are the most promising oilseed raw material for the production of natural oils by the method of cold pressing that are in high demand in cosmetology, for example, for the production of moisturizing cream for hands and feet based on chemically pure glycerin.

Blackberry Seeds-Derived Carbon as Stable Anodes for Lithium-Ion Batteries.

The suitability of biocarbons derived from blackberry seeds as anode materials in lithium-ion batteries has been assessed for the first time. Blackberry seeds have antibacterial, anticancer, antidysentery, antidiabetic, antidiarrheal, and potent antioxidant properties and are generally used for herbal medical purposes. Carbon is extracted from blackberries using a straightforward carbonization technique and activated with KOH at temperatures 700, 800, and 900 °C. The physical characterization demonstrates that activated blackberry seeds-derived carbon at 900 °C (ABBSC-900 °C) have well-ordered graphene sheets with high defects compared to the ABBSC-700 °C and ABBSC-800 °C. It is discovered that an ABBSC-900 °C is mesoporous, with a notable Brunauer-Emmett-Teller surface area of 65 m2 g-1. ABBSC-900 has good electrochemical characteristics, as studied under 100 and 1000 mA g-1 discharge conditions when used as a lithium intercalating anode. Delivered against a 500 mA g-1 current density, a steady reversible capacity of 482 mA h g-1 has been achieved even after 200 cycles. It is thought that disordered mesoporous carbon with a large surface area account for the improved electrochemical characteristics of the ABBSC-900 anode compared to the other ABBSC-700 and ABBSC-800 carbons. The research shows how to use a waste product, ABBSC, as the most desired anode for energy storage applications.

Proteins from Blackberry Seeds: Extraction, Osborne Isolate, Characteristics, Functional Properties, and Bioactivities.

Blackberry fruit contains high levels of nutrients and phenolic compounds. Blackberry pomace accounts for 20~30% of its whole fruit during processing and is generally treated as fertilizer. Blackberry pomace has many seeds that contain carbohydrates, polyphenols, flavonoids, pectin, protein, and other bioactive nutrients. However, its functional properties and seed protein compositions have not been reported. We used a single-factor experiment, response surface, and Osborne isolate method to extract protein isolate, albumin, globulin, glutelin, and prolamin from blackberry seeds for the first time and evaluated their characteristics and functional properties. Glutelin and protein isolate showed good water-holding capacity, emulsification, and foaming capacity, while albumin and globulin showed good oil-holding capacity and thermal stability. They were found to have good antioxidant activities that might be good DPPH free radical scavengers, especially prolamin, which has the lowest IC50 value (15.76 μg/mL). Moreover, globulin had the lowest IC50 value of 5.03 μg/mL against Hela cells, 31.82 μg/mL against HepG2 cells, and 77.81 μg/mL against MCF-7 cells and a high selectivity index (SI), which suggested globulin had better anti-cervical, antihepatoma, and anti-breast activity but relatively low cytotoxicity. These seed proteins may have great prospects for the development and application of food and drugs in the future.

Optimization of the Green Chemistry-like Extraction of Phenolic Compounds from Grape (Vitis labrusca L.) and Blackberry (Rubus fruticosus L.) Seeds with Concomitant Biological and Antioxidant Activity Assessments.

The objective of this work was to determine the phenolic composition, chemical and cellular antioxidant activity, cytotoxicity in human cells, and peroxidative inhibition of the defatted fraction of grape (Vitis labrusca) and blackberry (Rubus fruticosus) seeds. Soxhlet extraction (Sox) was used to extract the fat and obtain the degreased material. A statistical optimization study was developed to maximize the extraction of bioactive compounds and antioxidant activity from defatted grape and blackberry seeds. Simultaneous optimization was applied with a combination of 35.9 min of extraction and a solid-to-solvent ratio of 1 g of defatted grape seed to 61.28 mL of an extracting solvent (60% ethanol) and 62.1 min of extraction and a solid-to-solvent ratio of 1 g of defatted blackberry seed to 64.1 mL of an extracting solvent (60% ethanol). In the cell viability assay, HepG2 cancer cells seemed more sensitive to grape and blackberry extracts, while Ea.hy926 hybrid cells showed more resistance to their effects. In general, the extracts presented low/no cytotoxicity, exhibited a protective effect against H2O2-induced ROS production, and demonstrated antioxidant activity and a protective effect on the erythrocytes when subjected to hypotonic and isotonic conditions not presenting hemolytic behavior (5.0 to 10.0 μg GAE/mL). Thus, the results provided a broad assessment of the bioactivity of the extracts obtained using a simple and low-cost process developed by employing non-toxic solvents and with the potential to be used in technological applications.

Ultrasound-Assisted Extraction of Blackberry Seed Oil: Optimization and Oil Characterization

Ultrasound-assisted extraction (UAE) was applied to extract oil from blackberry (BB) seeds. The effect of UAE conditions on oil recovery and quality was investigated. Favorable experimental conditions (ultrasound intensity (UI), extraction temperature, and time) were investigated using response surface methodology (RSM). A Box-Behnken design was used to predict optimized conditions for BB seed oil extraction. These conditions were as follows: 13.77 W/cm2 UI, 45 °C extraction temperature, and 15 min extraction time. The experimental value obtained for extraction efficiency under optimal conditions was 87 ± 0.34%, in good agreement with the optimized predicted value. UAE does not affect the oil composition and confers higher antioxidant values in BB seed oil in comparison with Soxhlet extraction.

Influence of the biologically active supplement "Immunocort" on the production and quality of the "Mozzarella Ukrainian" cheese

Experimental studies have proven the effectiveness of the use of the herbal dietary supplement "Immunocort" in the technology of rennet cheese "Mozzarella Ukrainian". "Immunocort" is an herbal preparation that contains a balanced complex of immunoactive plants aimed at strengthening the immune system and eliminating immunodeficiency. The composition of the dietary supplement includes astragalus herb, echinacea root, Damascus blackberry seeds, currant leaf, string grass, Uncaria bark, and ant tree bark. Based on organoleptic and physical-chemical studies, it was established that the best option in the manufacture of "Mozzarella Ukrainian" cheese is to add to the normalized mixture before its pasteurization of the "Immunocort" supplement in the amount of 3 % by weight of the normalized mixture. A study was conducted on the effect of the coagulation temperature and pasteurization temperature on the synergistic properties of the cheese clot. These indicators confirmed the significant importance for the transition of solids into whey in the process of making rennet cheese "Mozzarella Ukrainian" with the bio additive "Immunocort". It was found that with an increase in temperature, there was a decrease in the syneretic ability of cheese clots. The efficiency of using solids increased with increasing pasteurization temperature. The relatively low content of dry substances in whey at high pasteurization temperatures can be explained by the denaturation of whey proteins and their transition to a cheese clot. The best results were achieved at the pasteurization temperature (86±2) °C and the coagulation temperature (32±2) °C. The data obtained could make it possible to manage the process of making new types of cheeses with vegetable additives, depending on the specific conditions and requirements for the product

Composite of α-FeOOH and Mesoporous Carbon Derived from Indian Blackberry Seeds as Low-Cost and Recyclable Photocatalyst for Degradation of Ciprofloxacin

This study aims to analyse the use of biowaste-derived carbon in enhancing the photocatalytic effect of Earth-abundant visible light active goethite (α−FeOOH). The biowaste material used in this case is seeds of the Indian blackberry fruit. The FeOOH/C composite has been synthesized using an assisted sonochemical technique. The photocatalysts have been characterized using powder x-ray diffraction, nitrogen adsorption isotherms and scanning electron microscopy technique. FTIR and Raman studies have been carried out to understand the structure bonding correlation. The band gap has been ascertained using Tauc plots. The adsorption and consequent photodegradation of CIP have been studied via UV-visible spectroscopy and the mechanism has been ascertained by using radical quenching techniques. The charge separation efficiency has been ascertained through photoluminescence (PL) studies and electrochemical impedance studies (EIS). The pivotal role played by photogenerated holes (h+) in the photocatalytic degradation of CIP has been highlighted. The low cost biowaste-derived carbon as a constituent of the FeOOH/C composite shows great promise as a supporting material for enhancing the photocatalytic properties of such semiconductor materials.

Postprandial Glycemic Response to Whole Fruit versus Blended Fruit in Healthy, Young Adults.

While increased intake of dietary fiber is known to reduce postprandial glycemic response, it is less understood whether the disruption of dietary fiber, in a blender, alters the postprandial glycemic response. We compared the postprandial glycemic response in 20 young, healthy college students (12 female, 8 male) after consuming whole fruit vs. blended fruit. The fruit included gala apple, with the seeds removed, and blackberries. We used a repeated measures two-way ANOVA with fruit treatment as the within-subject variable, sex as the between-subjects factor, and glucose maximum, glucose incremental area under the curve (iAUC), and 60 min glucose as dependent variables. Glucose maximum and glucose iAUC were significantly lower (p < 0.05) in blended fruit compared to whole fruit and 60 min glucose was marginally significantly lower (p = 0.057) in blended fruit compared to whole fruit. Sex was not a significant main effect and sex*treatment was not a significant interaction for any of the dependent variables. We hypothesize that a reduced glycemic response in blended apple and blackberries compared to whole apple and blackberries might be associated with the release of dietary fiber and nutritive components from ground blackberry seeds.

Chemical Composition, Antioxidant, Antimicrobial and Cytotoxic/Cytoprotective Activity of Non-Polar Extracts of Grape (Vitis labrusca cv. Bordeaux) and Blackberry (Rubus fruticosus) Seeds.

The aim of this study was to compare the influence of the extraction method, chemical composition, antimicrobial effects, antioxidant activity, and cytotoxicity on human cells of the non-polar extracts of grape (Vitis labrusca) and blackberry (Rubus fruticosus) seeds. The Soxhlet (Sox), Bligh–Dyer (BD), and ultrasound (US) methods were used for extractions. For blackberry non-polar seed extract, extraction via the BD method showed the highest mean values of total phenolic content (TPC), expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (102.37 mg GAE/100 mL), and higher antioxidant activity in relation to the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, expressed in milligrams of gallic acid equivalent per 100 mL of non-polar seed extracts (11.50 mg AAE/100 mL), if compared with the Sox and US extractions. Similar results were obtained for the non-polar grape seed extracts, where BD extraction obtained the highest values for TPC (28.61 mg GAE/100 mL) and DPPH (35.36 mg AAE/100 mL). The type of extraction method had an impact on the composition of fatty acids. Only the non-polar blackberry and grape seed extracts obtained via the Sox method showed some in vitro inhibitory effect against Escherichia coli (IAL 2064) and Staphylococcus aureus (ATCC 13565). Regardless of the extraction method used, the non-polar blackberry and grape seed extracts did not decrease the cell viability (IC50 >1000 µg/mL) of cancer and normal cell lines, thus indicating the relative safety of the extracts. All the seed extracts decreased the generation of reactive oxygen species in the cell lines. Blackberry and grape seed lipid fractions can be utilized as antioxidants, and the extraction methods used cause significant changes in relation to their bioactivity and chemical composition.

Extraction and Characterization of the Polar Lipid Fraction of Blackberry and Passion Fruit Seeds Oils Using Supercritical Fluid Extraction

The study of the phytochemical composition of seed oils is of upmost importance for the food and cosmetic industries, mainly considering their associated biological properties. Extraction of seed oils using supercritical fluids (SFE) is an ecological and green alternative to conventional extraction processes since it is able to provide with potent bioactive extracts, avoiding degradation and transformation of the compounds present originally in the raw material. The objective of the present work was the extraction of pure fractions of polar lipids and their chemical characterization using chromatographic techniques such as GC-MS and LC-DAD-MS/MS of blackberry (Rubus glaucus) and passion fruit (Passiflora edulis) seed oils extracted by supercritical carbon dioxide. Oleamides derived from oleic acid were identified as the main compounds in both samples; in particular, 9-octadecenamide was the major identified oleamide. Besides, the extract obtained from passion fruit showed to be a source of linoleic acid, while the SFE extract from blackberry presented important concentrations of vanillin. The chemical composition of these seed oils can be of high interest for their further use in cosmetics and food industry.

Investigation of Tocopherol Biosynthesis in Blackberry Seeds (Rubus spp.)

Tocopherol (vitamin E) is widely recognized as a cellular antioxidant and can prevent oxidative damage during seed quiescence and dormancy in all angiosperms. Tocopherols biosynthesis was highly accumulated in blackberry seeds, but limited study was done on expression of critical genes involved in this biosynthesis pathway. In the present study, each RuHPT and RuTMT cDNA sequence was isolated from boysenberry (Rubus idaeus × fruticosus × loganobaccus Darrow) and their expression patterns and regulation of tocopherols contents were investigated during different seed development stages from 14 to 42 days. RuHPT contained an open reading frame (ORF) of 1224 bp that encoded 407 amino acid residues, and for RuTMT, an ORF of 1056 bp encoding 351 amino acid residues. Phylogenetic analysis indicated that RuHPT and RuTMT were closely related to other Rosaceae species, Rosa chinensis and Fragaria vesca, respectively. The transcript level of both RuHPT and RuTMT gradually increased and reached a maximum level in the seeds of late ripening stages, 35 days after flowering (DAF) and 42 DAF. Assay of total tocopherol, α-tocopherol, γ‑tocopherol and δ-tocopherol contents by HPLC all revealed a similar changing trend concomitantly with the rising expression of RuHPT, and reached the peak on Day 35. The α-tocopherol level showed itself a trend of descending after ascending and reached its peak on Day 28. Therefore, RuHPT and RuTMT may both play a role in tocopherols biosynthesis in the seeds of ripening fruits. These results provide a novel foundation for investigating the functions of key enzyme genes on tocopherols biosynthesis in blackberry plants.

An immunomodulatory polysaccharide from blackberry seeds and its action on RAW 264.7 cells via activation of NF-κB/MAPK pathways

ABSTRACT Currently there is tremendous interest in the discovery of natural polysaccharide immunomodulators for treating immune-related diseases. Herein, the immunomodulatory activities of a polysaccharide fraction isolated from blackberry seeds (BSP-2) were examined in RAW264.7 cells. The results revealed that BSP-2 at 37.5–300 µg/mL could significantly enhance the phagocytic activity of RAW264.7 cells and their ability to release NO, TNF-α and IL-6 cytokines. BSP-2 could significantly up-regulate the mRNA expression of iNOS, TNF-α and IL-6, as well as the phosphorylation of p65, p38, ERK and JNK proteins. The mechanisms underlying the immunomodulatory actions of BSP-2 likely involve the activation of the NF-κB and MAPK signaling pathways and up-regulation of the phosphorylation of key proteins involved in these pathways. These findings suggest that BSP-2 could be applied as a polysaccharide immunotherapeutic adjuvant with the effective dose as low as 70 µg/mL (BSP-2 may be cytotoxic at levels > 600 μg/mL). Highlights Immunostimulatory potential of a blackberry seed polysaccharide (BSP-2) was examined. BSP-2 at 37.5–300 µg/mL could enhance the phagocytic activity of RAW264.7 cells. BSP-2 could significantly up-regulate the mRNA expression of iNOS, TNF-α and IL-6. BSP-2 up-regulated the phosphorylation of p65, p38, ERK and JNK proteins. Immunostimulatory BSP-2 acted via activating the NF-κB and MAPK signalling pathways.

Assessment of Bioactive Activity of Blackberry Seed Extract

Assessment of Bioactive Activity of Blackberry Seed Extract

TRIBOLOGICAL PROPERTIES OF BLACKBERRY WASTE SEED EXTRACT OBTAINED UNDER SUPERCRITICAL CO2 CONDITIONS WITH SORBITAN ESTERS COMPOSITONS

This paper presents results of tribological research of a composition containing as a base, extract of waste blackberry seeds obtained by extraction under supercritical carbon dioxide conditions with sorbitan esters as additives (sorbitan trioleate (STO) sorbitan monolaurate (MLS) and etoxylated sorbitan monolaurate (EO20MLS)). The influence of the concentration of surfactants on the motion resistance, wear and anti-seize properties of the obtained lubricants was analysed. Two types of tests were performed: seizure, with linear load increase and constant load (four-ball device T02). The obtained test results compared to the results for the base (pure extract) to paraffin oil and rapeseed oil were related. It was found that the addition of sorbitan esters to the extract has a greater effect on the improvement of anti-seizure properties than on the reduction of resistance to movement and wear to the base.

Chemical Composition of Blackberry Press Cake, Polyphenolic Extract, and Defatted Seeds, and Their Effects on Cecal Fermentation, Bacterial Metabolites, and Blood Lipid Profile in Rats.

Blackberry fiber (BF), extracted blackberry fiber (EBF), defatted blackberry seeds (DBS), and blackberry polyphenols (BP) were added to the diets of rats. A control diet, fiber diets (BF, EBF, DBS) with 6% addition instead of cellulose, and a polyphenol diet (BP) with 1% addition were administered for 4 weeks. BF and DBS contained polyphenols (3.6%); EBF did not. DBS was free of fat. Ellagitannins were the main phenolics, mostly dimers (sanguiin H-6 with isomers) and monomers (pedunculagin, casuarictin isomer, and sanguiin H-2 isomer). Trimers (lambertianin C with isomers) and tetramers (lambertianin D) were in the minority. EBF increased the production of propionate and butyrate in the cecum and improved the blood lipid profile. Polyphenols beneficially decreased the activity of cecal β-glucuronidase, but they may have also increased cholesterol levels in blood. Bacterial metabolism of ellagitannins in the colon gave rise to nasutin A and urolithin A.

Antithrombotic mechanism of polysaccharides in Blackberry (Rubus spp.) seeds

ABSTRACTThe blackberry seed was typically removed as a byproduct and waste from blackberry fruits for juices. Developing value-added utilization of berry seeds will significantly expand the market for berry products as well as improve benefit to berry producers. However, the research on blackberry seed is limited. The objective of this paper was to research antithrombotic mechanism of polysaccharides in blackberry seeds. Polysaccharides in blackberry seeds were extracted, purified and identified by high-performance gel permeation chromatography (HPSEC), gas chromatography (GC), fourier transform infrared (FT-IR) spectrometer and nuclear magnetic resonance spectra (NMR). Anticoagulant activities were evaluated in vivo by measuring activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), fibrinogen (FIB) and plasma recalcification time (RRT). Four polysaccharides named BSP-1a, BSP-1b, BSP-2 and BSP-3 were isolated from Blackberry (Rubus spp.) seeds. The results indicated that BSP-1b, BSP-2 and BSP-3 exhibited the anticoagulant activity. Therefore, the anti-thrombosis effects of BSP-1b, BSP-2 and BSP-3 were investigated in vivo by 6-Keto-PGF1α, thromboxane B2 (TXB2), endothelial nitric oxide synthase (eNOS), endothelin-1 (ET-1), whole blood viscosity (WBV), plasma viscosity (PV), hematocrit (Hct), erythrocyte sedimentation rate (ESR), APTT, TT, PT and FIB. The results suggested that BSP-1b, BSP-2 and BSP-3 had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect.

Characteristics of blackberry and raspberry seeds and oils

This study is concerned with the determination of technological quality characteristics of dried pomaces, i.e. blackberry and raspberry seeds, along with the quality parameters, content of total carotenoids and chlorophyl and transparency of crude extracted oil (using organic solvent). Blackberry seeds (Rubus fruticosus L.) were obtained from a domestic variety Cacanska bestrna, while the raspberry seeds (Rubus idaeus L.) were of the variety Willamette. Oil content of the blackberry pomace was 13.97 and 14.34%, while the oil content of the raspberry pomace was 13.44 and 14.33% on dry basis (d.b.). In regard to technological characteristics of the pomaces, i.e. volumetric and specific weight, no considerably difference was found. However, a weight test for 1000 seeds showed a significant difference in weight: 3.5 g (d.b.) for the blackberry pomace and 1.5 g for the raspberry pomace (d.b.). Proximate analysis of blackberry seed oil showed that this oil had better quality since the FFA value was 3.43% (sample B1) and 3.53% (sample B2), while the peroxide value was 8.89 and 11.16 mmol/kg, respectively. Raspberry seed oil had higher FFA (8.59 and 8.83% for sample R1 and R2) and peroxide values (13.99 and 13.84 for sample R1 and R2) than the blackberry seed oil. Crude extracted blackberry seed oil had a brown-greenish color due to the high total chlorophyll content (around 3000 mg/kg dissolved in cyclohexane). Raspberry seed oil had a dark yellowishorange color, due to lower chlorophyll content (around 200 mg/kg) compared to the blackberry seed oil, while the content of total carotenoids was slightly higher in this oil (around 40 mg/kg) compared to the blackberry seed oil (33 mg/kg).