Black Heart Research Articles

基于透射光谱技术的马铃薯种薯黑心病检测研究

Black heart disease is one of the screening indicators of seed potatoes, which has a serious impact on the quality and yield of potato, and at present there are fewer non-destructive testing methods for internal defects of seed potatoes. This paper aims to utilize non-destructive testing technology to quickly identify qualified and black hearted seed potatoes, and then to protect yield and food security. In this paper, transmission spectroscopy system was utilized to collect the spectral data of 104 qualified seed potatoes and 104 black hearted seed potatoes in 450~940 nm band. Subsequently, four algorithms, namely Savitzky-Golay (SG), Standard Normal Variate (SNV), Multiplicative Scatter Correction (MSC) and First-order Derivative (FD), were utilized to pre-process the seed potatoes spectral data to improve the data noise ratio. Feature wavelength extraction was made using Competitive Adaptive Reweighted Sampling (CARS) and Successive Projections Algorithm (SPA) to enhance the sample data characteristics and improve the model interpretability. The construction of classification models for qualified and black hearted seed potatoes relied on two deep learning techniques, Convolutional Neural Networks (CNN) and Recurrent Neural Network (RNN), which were trained and tested for the feature bands respectively. The experimental results showed that SG-CARS-CNN was the optimal combination of classification algorithms, and the classification accuracies of both the training set and the test set reached 100%, which improved the accuracy of the test set by 3.85% compared with that of the traditional machine learning algorithms, and provided an accurate method for the rapid screening of qualified seed potatoes.

Research on the Harmonious Development of China's Old-age Care Institutions

With the development of socialization, old-age care institutions have been paid attention to in the old-age security system. After the occurrence of the "Black Heart Retirement Home" incident, we have to consider the imbalances in the development of old-age care institutions. Analysis of the current imbalance and its causes, hoping to find a solution to the problem of imbalance, to effectively prevent the occurrence of similar incidents, and solve the worry of the elderly.

(714) - Primary Graft Failure in the Black Heart Transplant Recipient

(714) - Primary Graft Failure in the Black Heart Transplant Recipient

Effect of trace elements on mycelial growth and sporulation of Alternata alternata (Fr.) Keissler causing black heart of pomegranate

Effect of trace elements on mycelial growth and sporulation of Alternata alternata (Fr.) Keissler causing black heart of pomegranate

Bacillus safensis filtrate-based ZnO nanoparticles control black heart rot disease of apricot fruits by maintaining its soluble sugars and carotenoids.

Black heart rot is a serious disease of apricot and it has been reported to be caused by Alternaria solani, around the world. The present research was designed to control this disastrous disease using zinc oxide nanoparticles (b-ZnO NPs). These NPs were synthesized in the filtrate of a useful bacterium (Bacillus safensis) and applied to control black heart rot of apricot. After synthesis, the reduction of b-ZnO NPs was confirmed by UV-visible spectroscopy, at 330nm. Fourier transform infrared (FTIR) spectra ensured the presence of multiple functional groups (alcohols, phenols, carboxylic acids, nitro compounds and amines) on the surface of b-ZnO NPs. X-Ray diffraction (XRD) analysis elucidated their average size (18nm) while scanning electron microscopy (SEM) micrograph described the spherical shape of b-ZnO NPs. The synthesized b-ZnO NPs were applied in four different concentrations (0.25mg/ml, 0.50mg/ml, 0.75mg/ml, 1.0mg/ml) under both in vitro and in vivo conditions. These NPs were very efficient in inhibiting mycelial growth (85.1%) of A. solani at 0.75mg/ml concentration of NPs, in vitro. Same concentration also performed best, in vivo, and significantly reduced disease incidence (by 67%) on self-inoculated apricot fruit. Apart from this, application of b-ZnO NPs helped apricot fruit to maintain its quality under fungal-stress conditions. The decay of apricot fruit was reduced and they maintained greater firmness and higher weight. Moreover, b-ZnO NPs treated fruits controlled black heart rot disease by maintaining higher contents of ascorbic acid, soluble sugars and carotenoids. These b-ZnO NPs were produced in powder form for their easy carriage to the farmers' fields.

Assessment of vitamin- and mineral-content stability of tomato fruits as a potential raw material to produce functional food

Background: Tomatoes are a valuable source of biologically active substances (vitamin C, lycopene, lutein, macro- and microelements) and can be used to produce functional foods.Objective: Nine varieties of tomato fruits, featuring a spectrum of colors such as red, yellow, and dark hues (brown, burgundy), were examined. These tomatoes were cultivated in open ground in both the Russian Federation and the Republic of Belarus. Methods: The mineral composition of tomato fruit samples was determined using an atomic emission spectrometer with inductively coupled plasma, mass fractions of vitamin C and carotenoids were determined by high-performance liquid chromatography.Results: The highest vitamin C content was observed in the fruits of tomato varieties Viagra and Chocolate, cultivated in the villages of Khokhol and Nikonovo in the Voronezh Region, Russia, and in the variety Budenovka, grown in Nikonovo. Additionally, the highest lycopene content was identified in the fruits of The Black Prince, cultivated in the agro-town Samokhvalovichi, Belarus; Volgogradskiy skorospelyi, grown in Nikonovo; Bull's Heart, cultivated in Khokhol in the Voronezh Region, Russia; and Black Heart of Bull, cultivated in Khokhol and Nikonovo respectively. Notably, tomatoes of the varieties The Black Prince (agro-town Samokhvalovichi, Belarus), Chocolate, Black Heart of Bull, and Bull's Heart (Khokhol, Russia) exhibited a significant amount of lutein. Furthermore, tomatoes of the variety Budenovka, grown in Khokhol, Russia, displayed high mineral saturation. Varieties Bull's Heart and Black Heart of Bull, grown in the same region, were found to be rich in macro- and trace elements (K, Ca, Mg, P, Mn, Fe). The optimal calcium-to-phosphorus ratio (1:1.5) was observed in varieties Bull's Forehead, Black Heart of Bull, and Budenovka, while the optimal calcium-to-magnesium ratio (1:0.7) was present in the fruits of tomato varieties Volgogradskiy skorospelyi, Bull's Forehead, Budenovka, Black Heart of Bull, Bull's Heart, and Orange, grown in various regions of the Russian Federation. Conclusion: The inclusion of biologically active substances, notably lycopene, lutein, Vitamin C, and essential minerals (Potassium, Calcium, Magnesium, Phosphorus), positions tomatoes as valuable sources for developing functional foods and those designed to prevent cardiovascular diseases. The composition of tomatoes varies significantly based on the fruit's color and the cultivation location. This variability underscores the importance of considering these factors to achieve the desired level of functional ingredients in the final product.Keywords: tomato fruits, biologically active substances, vitamin C, lycopene, lutein, macro- and microelements.

House Rent Prediction Method Based on Decision Tree: Take India as an Example

In this essay, this paper use decision trees to predict house rent and compare it to other linear regressions to find out why decision trees are a good fit for house rent and use house rent data 2023. This paper used the data set from Kaggle which has many factors and is up-to-date for my project, employing some visualization methods on Python to show these factors and their graphs so we can understand some important circumstances about house rent in India. This paper employed three different methods of training models to train them to predict their rent. In the last section, this paper use R2_score, RMSE, MAE and MSE to compare their conclusions. Thus, this paper show why decision trees are the best model for predicting house rent. After all process, this paper proposes that data rent price prediction is very important, because many people don’t have a good obedience on other things like other people’s advice or other black heart house property agents.

Beet Moth: Has Summer 2022 Introduced a New Threat to the UK Sugar Beet Crop?

When the first samples of ‘beet black heart’ arrived at the BBRO Plant Clinic in May 2022, the initial reaction was that it was due to downy mildew or boron deficiency, but as the long hot summer continued it became increasingly apparent some of these early samples were more likely to have been the first signs of beet moth damage. Although not a new problem (several isolated cases were recorded in commercial beet during 2020), the extent of the issue and the widespread impact on the crop took many by surprise. In this article we highlight some of the challenges caused by this pest and reflect on what the industry can do to limit its impact in the future. The beet moth (Scrobipalpa ocellatella) is not regarded as a regular pest in the UK, but adult moths are trapped and reported by UK entomologists. It is known to be more problematic in Mediterranean areas and across Eastern Europe/Asia where its incidence and reports of damage are usually associated with warmer and drier climates. Unfortunately, to date, we have limited information on its life cycle in relation to UK conditions and our sugar beet crops.

Is the Affordable Care Act Moving the Needle for Advanced Heart Failure Therapies for Black Patients?

Is the Affordable Care Act Moving the Needle for Advanced Heart Failure Therapies for Black Patients?

Improved 3 Year Heart Transplant Survival in Black recipients Following the Affordable Care Act.

To improve healthcare access, the US government implemented the Affordable Care Act (ACA) in 2014. Previous studies investigating its impact on healthcare inequities showed significant improvement in Black transplant recipient outcomes. Our objective is to determine the ACA's impact on Black heart transplant (HTx) recipients. Using the United Network for Organ Sharing database, we analyzed 3,462 Black HTx recipients pre- and post-ACA (January 2009 to December 2012, and January 2014 to December 2017). Black recipient numbers and rates of overall HTx, insurance effects on survival, geographic changes in HTx, and post-HTx survival were compared pre- and post-ACA. Black recipients increased from 1,046 (15.3%) to 2,056 (22.2%) post-ACA (p < 0.001). Three year survival increased among Black recipients (85.8-91.9%, p = 0.01; 79.4-87.7%, p < 0.01; 78.3-84.6%, p < 0.01). Affordable Care Act implementation was protective for survival (hazard ratio [HR] = 0.64 [95% confidence interval [CI], 0.51-0.81], p < 0.01). Publicly insured patient survival increased post-ACA to match that of privately insured (87.3-91.8%, p = 0.001). United Network for Organ Sharing (UNOS) Regions 2, 8, and 11 experienced improved survival post-ACA (p = 0.047, p = 0.02, and p < 0.01, respectively). The post-ACA era showed improved HTx access and survival in Black recipients, indicating that national medical policy may play a strong role in eliminating racial disparities. Further attention is required to improve inequities in medical care.

The Crucible

The Crucible

Racial disparities in post-transplant stroke and mortality following stroke in adult cardiac transplant recipients in the United States.

Black heart transplant recipients have a higher mortality rate than white recipients 6-12 months after transplant. Whether there are racial disparities in post-transplant stroke incidence and all-cause mortality following post-transplant stroke among cardiac transplant recipients is unknown. Using a nationwide transplant registry, we assessed the association between race and incident post-transplant stroke using logistic regression and the association between race and mortality among adults who survived a post-transplant stroke using Cox proportional hazards regression. We found no evidence of an association between race and the odds of post-transplant stroke (OR = 1.00, 95% CI: 0.83-1.20). The median survival time of those with a post-transplant stroke in this cohort was 4.1 years (95% CI: 3.0, 5.4). There were 726 deaths among the 1139 patients with post-transplant stroke, including 127 deaths among 203 Black patients and 599 deaths among 936 white patients. Among post-transplant stroke survivors, Black transplant recipients experienced a 23% higher rate of mortality compared to white recipients (HR = 1.23, 95% CI: 1.00-1.52). This disparity is strongest in the period beyond the first 6 months and appears to be mediated by differences in the post-transplant setting of care between Black and white patients. The racial disparity in mortality outcomes was not evident in the past decade. The improved survival of Black patients in the recent decade may reflect overall protocol improvements for heart transplant recipients irrespective of race, such as advancements in surgical techniques and immediate postoperative care as well as increased awareness about reducing racial disparities.

Abstract 9800: Racial Differences in Donor-Derived and Mitochondrial Cell-Free Dna Could Explain Inferior Outcomes in Black Heart Transplant Patients, on Behalf of the Graft Investigators

Introduction: Black heart transplant patients are at higher risk of acute rejection (AR) and death than White patients. Our group has shown that %donor-derived cell-free DNA (%ddcfDNA) is a sensitive and specific marker of AR in heart transplantation. Cell-free mitochondrial DNA (cfmtDNA) has been linked to allograft dysfunction and may mechanistically implicated in inferior outcomes for Black heart transplant recipients. Hypothesis: Higher levels of allograft injury when quantitated using %ddcfDNA and total cfmtDNA will be seen in Black heart transplant patients as compared to White patients. Methods: The Genomic Research Alliance for Transplantation (GRAfT) is a multicenter, prospective, longitudinal cohort study of heart transplant patients transplanted between 2015 and 2021. We included Black and White patients and shotgun sequencing was used to quantitate %ddcfDNA and polymerase chain reaction (PCR) to quantitate total cfmtDNA levels. AR was defined as grade ≥2R cellular rejection or pAMR ≥1 antibody-mediated rejection (AMR). Results: We included 151 patients (73 Blacks and 78 Whites), the median age was 56y (29% Female sex). Patients of both racial groups had comparable baseline characteristics. There were a ~1100 ddcfDNA and cfmtDNA measures included in the analysis. At 18-months, the incidence of AR was 2.8% in White patients and 9.2% in Black patients (p&lt;0.01). The diagnostic accuracy of %ddcfDNA to detect AR was comparable between Black and White patients (0.90 vs. 0.83, p=0.19). The %ddcfDNA and cfmtDNA was higher at serial time points after transplant in Black compared to White patients (Figure). CfmtDNA levels were also elevated with AMR which occurred almost exclusively in Black patients (No rejection: 146,345 copies/ul, AMR: 242,238 copies/ul; p=0.004). Conclusions: Elevated levels of %ddcfDNA and cfmtDNA after heart transplant may explain the higher incidence of AR, AMR, and inferior outcomes in Black heart transplant recipients.

Post-transplant mortality and graft failure after induction immunosuppression among Black heart transplant recipients in the United States

Black heart transplant recipients are more likely to receive induction immunosuppression compared to other races because of higher rates of acute rejection, graft failure, and mortality. However, it is not known whether contemporary induction immunosuppression improves their post-transplant outcomes. To evaluate whether Black patients who were prescribed induction immunosuppression therapy have lower all-cause mortality or graft-failure rates compared to those who were not, we studied Black U.S. adult heart transplant recipients in the Scientific Registry of Transplant Recipients database (2008-2018). We used multivariable Cox proportional hazards regression analysis to compare the hazards of all-cause mortality or graft failure as a composite, for patients who were prescribed induction immunosuppression and those who were not. Among 5160 recipients, 2787 (54.0%) were prescribed induction immunosuppression and 2373 (46.0%) were not. There was no evidence of survival differences according to induction immunosuppression for the composite of all-cause mortality or graft failure (aHR=1.13, 95% CI 0.96-1.32), mortality (aHR=1.14, 95% CI 0.97-1.34), graft failure (aHR=1.05, 95% CI 0.82-1.34) and acute rejection (aHR=1.00, 95% CI 0.89-1.12). Given the side effects of treatment, future guidelines should reconsider the recommendation for induction immunosuppression among Black patients.

Some biochemical changes and transcriptome analysis associated with ‘Queen’ pineapple fruit blackheart development

Blackheart (BH) disease is a major problem of pineapple (Ananas comosus L. ‘Comte de Paris’) during storage. In this paper, physiological and molecular responses to the blackheart (BH) disease of pineapple were determined during storage at room temperature (25 ℃). The results showed that the BH development of pineapple was accompanied by an increased rate of superoxide free radical (O2•−) production, increased malondialdehyde (MDA) content and polyphenol oxidase (PPO) activity, reduced the antioxidant enzymes activities of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX), and decreased amount of ascorbic acid (AsA) in flesh (F), flesh adjacent to the core (F/C) and core (C) tissues of pineapple. Meanwhile, a transcriptome analysis identified many differentially expressed genes (DEGs) associated with BH development. The up-regulated DEGs were significantly enriched in ribosome and the down-regulated DEGs were mainly related to the starch and sucrose metabolism. These results provide a theoretical basis for the mechanism of pineapple BH.

Postharvest Rot of Pomegranate Fruit in Southern Italy: Characterization of the Main Pathogens.

Pomegranate (Punica granatum L.) is an emerging crop in Italy and particularly in southern regions, such as Apulia, Basilicata, and Sicily, due to favorable climatic conditions. The crop is affected by several pathogenic fungi, primarily in the field, but also during postharvest phases. The most important postharvest fungal diseases in pomegranate are gray and blue molds, black heart and black spot, anthracnose, dry rot, and various soft rots. The limited number of fungicides allowed for treatment in the field and the lack of postharvest fungicides make it difficult to control latent, quiescent, and incipient fungal infections. Symptomatic pomegranates from southern Italy were sampled and isolated fungi were morphologically and molecularly characterized. The data obtained revealed that various species of Penicillium sensu lato (including Talaromyces genus), Alternaria spp., Coniella granati, and Botrytis cinerea were the principal etiological agents of postharvest pomegranate fruit diseases; other relevant pathogens, although less represented, were ascribable to Aspergillus sect. nigri, Colletotrichum acutatum sensu stricto, and Cytospora punicae. About two thirds of the isolated pathogens were responsible for latent infections. The results obtained may be useful in planning phytosanitary control strategies from the field to storage, so as to reduce yield losses.

Racial and Socioeconomic Disparities in Pediatric Heart Transplant Outcomes in the Era of Anti-Thymocyte Globulin Induction

<h3>Purpose</h3> A recent multicenter pediatric study using antithymocyte globulin (ATG) induction found a reduced risk of first yr rejection in Black heart transplant (tx) recipients. We investigated this finding in the broader field and studied the effects of race and socioeconomic disparity on outcomes in ATG-induced children. <h3>Methods</h3> Using the Pediatric Heart Transplant Society registry, we compared outcomes in Black and White children 1-17 yrs old who underwent heart tx with ATG induction between 2000-2020. The primary outcomes of treated rejection, rejection with hemodynamic compromise (HC), and graft loss (death or re-transplant) were studied over 1- and 10-yr time intervals as well as late outcomes conditional on 1-yr survival<i>.</i> We explored the association of these outcomes with race and socioeconomic disparity, assessed using neighborhood deprivation index [NDI] score at 1-yr post-tx (high NDI score implies more socioeconomic disadvantage). <h3>Results</h3> The study cohort included 1719 ATG-induced pediatric heart tx recipients (23% Black, 77% White). There was no racial difference in incidence of first yr treated rejection (Black 24.5%, White 28.1%, p = 0.2). During the 10 yr follow up, the risk of treated rejection was similar between Black and White recipients; however, Black recipients were at higher risk of HC rejection (p = 0.009) and graft loss (p = 0.02, log rank test). Black recipients had a higher mean NDI score (0.43 vs 0.36, p < 0.001). A higher risk of graft loss was associated with high NDI score in both White and Black recipients (p < 0.001, Fig). In a multivariable Cox model, both high NDI score (HR 2.03, 95% CI 1.38-2.98) and Black race (HR 1.99, 95% CI 1.36-2.93) were associated with graft loss. <h3>Conclusion</h3> Black race and socioeconomic disadvantage remain associated with HC rejection and graft loss after the first post-transplant year in pediatric heart tx patients with ATG induction. These disparities represent important opportunities to improve long term tx outcomes in children.

Microwave Scanning Technology as a Non-destructive Testing Method for Detecting Black Heart in Potato

In recent decades, food scientists and engineers have attempted to develop an improve technologies that can detect the internal damages of agricultural produce under postharvest conditions using a various methods of destructive and non-destructive technologies. This study presents the technique of microwave scanning as a non-destructive technology for application in agriculture to detect black heart in potato tubers in a non-destructive way. A bi-static antenna configuration of microwave scanning setup operating at 2.45GHz was assembled, with a customized slotted rectangular microstrip antenna designed and simulated with CST, thereafter fabricate the antenna to be used as a microwave sensor in the setup, a miniVNA tiny double as a RF generator and S-parameter measurement was used to measured transmission coefficient through samples of real potato, the analysis was based on the differences in the wave spectrum obtained between the good potato sample and the sample with black heart. The result obtained from the analysis of wave spectrum shows that there is a significant difference up to 5dB in the wave pattern of a good potato sample and the sample with internal damages. Accuracy up to 86% of true state of the tested sample was achieved using the technology. Hence, the method can be adequately used as a scientific way of grading and sorting a batch of potato non-destructively.

Mythological motives in the fairy tales of M. Vagatova

The Khanty writer Maria Kuzminichna Vagatova (Voldina) put into her fairy tales not only moral and ethical guidelines for the younger generation: what is good and evil, the need to respect nature and animals, but also the cultural layer of the indigenous people of the North, which consists of mythological motives. The tales of Maria Vagatova are studied in the national schools of Khanty-Yugra within the framework of the subject Native (Khanty) Literature, due to which students absorb a conscientious appeal to the world around them from childhood, and also study a significant part of Khanty mythology, which has not disappeared under the influence of modernity and forms the outlook of those who still honor the traditions of their ancestors. The relevance of this topic lies in its poor study. When looking for the necessary information, it was noticed that most often when considering the work of Maria Vagatova, researchers turn to her poetry, and not to fairy tales, and mostly in the aspect of comparison with the works of other Khanty writers. This explains the theoretical significance of this article. The practical significance implies the possible use of the analysis of mythological motives in the fairy tales of Maria Vagatova in the educational activities of national schools. The main content of the work is based on the analysis of mythological motives in the fairy tales of Maria Vagatova from the collection Tyoi, Tyoi: Hips-Vips, Hily and Aki Black Heart, Hare and Fox, Khlebushko, Bunny-Black Tail ... To identify these motives, elements of a system-holistic analysis of a work of art and a hermeneutic method were used.

Abstract 11003: Racial Disparities in Induction Immunosuppression Use Among Heart Transplant Recipients in the United States

Introduction: Black heart transplant recipients have higher rates of acute rejection, graft failure, and mortality compared to other races. Induction immunosuppression in the immediate peri-transplant period may lower the risk of acute rejection among high-risk patients, including Black transplant recipients. However, it is not known whether the odds of receiving this treatment are different across races. Therefore, we evaluated whether there are disparities in the use of induction immunosuppression following heart transplants among Black patients compared to other races. Methods: We conducted a cross-sectional study of U.S. adults in the Scientific Registry of Transplant Recipients who received their first heart transplant between 2008 - 2018. We used mixed-effects logistic regression to compare the odds of receiving induction immunosuppression agents (IL-2 receptor antagonists, polyclonal anti-lymphocyte antibodies, and CD52 monoclonal antibodies) across different races. Results: Among 23,935 adult heart transplant recipients, 5,160 (21.6%) were Black, including 2,797 (54.2%) who received induction immunosuppression, and 18,775 (78.4%) were non-Black, including 9,499 (50.6%) who received induction immunosuppression. After accounting for demographic and clinical characteristics and treatment center, Black race was associated with 28% higher odds (Odds Ratio=1.28, 95% CI 1.17-1.40) of induction immunosuppression use compared to other races. Similar results were observed when race was further stratified (Table 1). Conclusions: In our study, Black heart transplant recipients were more likely to receive induction immunosuppression than transplant recipients of other races. However, further research on post-transplant clinical outcomes including survival following induction immunosuppression among Black patients is needed to formulate treatment guidelines.