Blackfly Fauna Research Articles

Contribution to the Occurrence of the Blackfly (Diptera:Similiidae) in the Palestinian Territories, West Bank

Aims: The primary objective of the study was to gather evidence regarding the presence of black fly fauna in the Palestinian environment. The research extensively examined breeding sites and habitats, as well as studied the physiochemical parameters of the water in these breeding sites. Furthermore, the study assessed the potential impacts of these insects on both humans and animals
 Study Design: A cross- sectional study.
 Place and Duration of Study: From January 2023 to August 2023, a cross-sectional survey was conducted in the West Bank-Salfit district to collect larvae and adult blackflies within the Wadi Qana protected area.
 Methodology: In January 2023, a field survey was conducted in the Wadi Qana protected area (32.12582, 34.8952) in the Salfit district to study the freshwater fauna. A total of 220 larvae individual of the Blackfly were collected from the running water in the valley. Forceps used to collect specimens and preserved in 70% ethanol, while adults of this group were examined under a dissecting microscope. The specimens were identified using various identification keys [4,9,12].
 Results: During the study period, a total of 220 larvae were collected from breeding sites in the freshwater habitats of Wadi Qana. Two subgenera of blackflies were identified in the study area: Simulium (Wilhelmia) Enderlein and Simulium (Odgamia) Enderlein. The results indicate that blackflies breed in fast-flowing freshwater habitats covered by annual aquatic plants, with dissolved oxygen levels at 10.1 mg/L, pH average 8.2 and average temperature 21.6°C.
 No significant health effects were recorded or documented in the study area, except for the nuisance caused to humans and animals by the biting of blackflies.
 In conclusion, this study marks the first record and presence of the blackfly in the Palestinian environment. Further much more research is needed to explore their fauna, biology, ecology, habitats, distribution, and medical significance.
 Conclusion: blackfly was recorded for the first time in the Palestinian environment, more study research are needed to discussed the fauna, biology, ecology, habitats, distribution and the medically important.

From bites to barcodes: uncovering the hidden diversity of black flies (Diptera: Simuliidae) in Vietnam

BackgroundPrompt and precise identification of black flies (Simuliidae) is crucial, given their biting behaviour and significant impact on human and animal health. To address the challenges presented by morphology and chromosomes in black fly taxonomy, along with the limited availability of molecular data pertaining to the black fly fauna in Vietnam, this study employed DNA-based approaches. Specifically, we used mitochondrial and nuclear-encoded genes to distinguish nominal species of black flies in Vietnam.MethodsIn this study, 135 mitochondrial cytochrome c oxidase subunit I (COI) sequences were established for 45 species in the genus Simulium in Vietnam, encompassing three subgenera (Gomphostilbia, Nevermannia, and Simulium), with 64 paratypes of 27 species and 16 topotypes of six species. Of these COI sequences, 71, representing 27 species, are reported for the first time.ResultsCombined with GenBank sequences of specimens from Malaysia, Myanmar, Thailand, and Vietnam, a total of 234 DNA barcodes of 53 nominal species resulted in a 71% success rate for species identification. Species from the non-monophyletic Simulium asakoae, S. feuerborni, S. multistriatum, S. striatum, S. tuberosum, and S. variegatum species groups were associated with ambiguous or incorrect identifications. Pairwise distances, phylogenetics, and species delimitation analyses revealed a high level of cryptic diversity, with discovery of 15 cryptic taxa. The current study also revealed the limited utility of a fast-evolving nuclear gene, big zinc finger (BZF), in discriminating closely related, morphologically similar nominal species of the S. asakoae species group.ConclusionThis study represents the first comprehensive molecular genetic analysis of the black fly fauna in Vietnam to our knowledge, providing a foundation for future research. DNA barcoding exhibits varying levels of differentiating efficiency across species groups but is valuable in the discovery of cryptic diversity.Graphical abstract

DNA Barcoding of Black Flies (Diptera: Simuliidae) in Slovakia and Its Utility for Species Identification

DNA barcoding based on the cytochrome oxidase I gene is increasingly used in black flies (Diptera: Simuliidae), but extensive data for larger areas are still rare. Slovakia, with well-explored black fly fauna, was chosen to verify the reliability of DNA barcoding for species identification. The DNA barcoding region of the COI gene of 235 individuals of 25 black fly species from Slovakia was sequenced. Among them, 30 sequence clusters with assigned Barcode Index Numbers (BINs) were identified, and 5 of them were recorded for the first time. The average intraspecific genetic divergence was 0–3.24%, whereas the average interspecific divergence was 12.3–17.8%. Based on the barcode sequence, 14 species could be identified unambiguously, and 3 of them (Prosimulium latimucro, Simulium costatum, S. degrangei) are split into two or more barcode clusters. In eleven species, some degree of barcode sharing occurred, often combined with barcode splitting. The results showed hidden diversity as well as cases of shared barcode sequences among the studied species. Further investigation using other molecular markers is necessary due to the overlap of intraspecific and interspecific variability.

First record of black scavenger fly of the genus Meroplius Rondani, 1874 (Diptera: Sepsidae) from Pakistan

A new record is added to the black scavenger fly fauna of Pakistan by the reporting of Meroplius minutus (Wiedemann, 1830), a rare species from Rawalakot, Azad Jammu & Kashmir, Pakistan. The genus is also a new record for the country. Diagnostic characters of both the genus and the species are provided in detail with the help of images. In addition, detailed distribution notes and information about their habitats are provided.

DNA barcoding of blackflies (Diptera: Simuliidae) as a tool for species identification and detection of hidden diversity in the eastern regions of Spain

BackgroundBlackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately.MethodsBlackflies specimens (larvae, pupae, reared adults and biting females) were collected in the period 2015–2017 in and near rivers and streams from different regions in Spain. A modified Hotshot technique was used for the DNA extraction and the cox1 DNA barcoding region of the cytochrome c oxidase subunit 1 was sequenced from the specimens collected.ResultsIn total, we collected 239 specimens representing 22 species. Of these, six species are new records for the Aragón region: P. tomosvaryi, S. bertrandi, S. galloprovinciale, S. lineatum, S. rubzovianum and S. xanthinum. Cox1 DNA barcode sequences for 21 species were recovered, including four species of the genus Prosimulium and 17 species of the genus Simulium [Boophthora (1 species), Eusimulium (1 species), Nevermannia (4 species), Simulium (s.s.) (6 species), Trichodagmia (1 species) and Wilhelmia (4 species)]. For the first time the complete DNA barcodes for five species (P. tomosvaryi, S. carthusiense, S. brevidens, S. monticola and S. sergenti) were registered. Most of the specimens belonging to the same recognized species were clustered together in the neighbour-joining tree, except for S. argyreatum, S. monticola and S. variegatum. The overall genetic distance in the dataset was 0.14%. The average of the intraspecific genetic divergence within the different taxa was 1.47% (0.05–3.96%). In contrast, the interspecific divergence varied between 2.50–22.0%.ConclusionsIn this study we assessed the use of the cox1 DNA barcoding region for the identification of species of blackflies in Spain. Our results showed that combining DNA barcoding with morphology enhanced our taxonomic rationale in identifying the blackflies in the country.

The black flies (Diptera: Simuliidae) of Vietnam.

The biodiversity of black flies (Diptera: Simuliidae), which are biting insects of medical and veterinary importance, is strikingly high in Southeast Asian countries, such as Indonesia, Malaysia, Philippines and Thailand. In 2013, we began to explore the fauna of black flies in Vietnam, which has so far been poorly studied. In this monograph, the wealth of the biodiversity of black flies in Vietnam is also confirmed on the basis of the results of our recent investigations, though limited to five provinces in the country. Morphotaxonomic studies of black flies obtained from Sapa, Lao Cai Province, northern Vietnam, in 2014 and Nghe An Province, northern Vietnam, in 2015, and reexaminations of black flies collected from Tam Dao, Vinh Phuc Province, northern Vietnam, in 2013, Thua Thien Hue Province, central Vietnam, in 2014, and Lam Dong Province, southern Vietnam, in 2014, were conducted. A total of 22 species are described as new, including one in the newly recorded subgenus Montisimulium Rubtsov, and three species are recognized as new records from Vietnam. This investigation brings the number of species of black flies known in Vietnam to 70, all of which are assigned to the genus Simulium Latreille, and are placed in four subgenera (25 in Gomphostilbia Enderlein, one in Montisimulium, seven in Nevermannia Enderlein, and 37 in Simulium Latreille s. str.). The numbers of species-groups recognized include seven in Gomphostilbia, three in Nevermannia and nine in Simulium, indicating a high diversity of putative phylogenetic lineages. New species include S. (G.) sanchayense sp. nov. (= the species formerly regarded as S. (G.) brinchangense Takaoka, Sofian-Azirun & Hashim), S. (S.) lowi sp. nov. (= the species formerly regarded as S. (S.) brevipar Takaoka & Davies), S. (S.) fuscicoxae sp. nov. [= the species formerly regarded as S. (S.) rufibasis Brunetti (in part)], S. (S.) suoivangense sp. nov. [= morphoform 'b' of the S. (S.) tani Takaoka & Davies (complex)]. Newly recorded species are S. (G.) parahiyangum Takaoka & Sigit, S. (N.) maeaiense Takaoka & Srisuka, and S. (S.) doipuiense Takaoka & Choochote (complex) [= the species formerly regarded as S. (S.) rufibasis Brunetti (in part)]. The substitute name, S. (S.) huense, is given for the species that was described under the name of S. (S.) cavum from southern Vietnam. A redescription of the female, male, pupa and larva of S. (G.) asakoae Takaoka & Davies is presented, and the female and larva of S. (G.) hongthaii Takaoka, Sofian-Azirun & Ya'cob are described for the first time. Keys to 10 subgenera in the Oriental Region and all 70 species recorded from Vietnam are provided for females, males, pupae and mature larvae. As investigations extend nationwide in all the provinces in Vietnam, more new species and records are expected to be discovered. It is hoped that this monograph will be useful as a baseline taxonomic reference for future studies of black flies in Vietnam and neighbouring countries.

The black flies (Diptera: Simuliidae) of the Lesser Sunda Archipelago, Indonesia

Surveys of pupae and larvae of black flies were carried out in Bali, Lombok, Sumbawa and Flores in the Lesser Sunda Archipelago, Indonesia, where 10 species were known. A total of 14 simuliid species including four new species and five new records of the genus Simulium were collected, bringing the number of species from the Lesser Sunda Archipelago to 19. They are classified into four subgenera: two in Nevermannia, nine in Gomphostilbia, seven in Simulium and one in Wallacellum. One of four new species, Simulium (Simulium) baliense, is described based on females, males, pupae and larvae from Bali and Lombok. This new species, which is placed in the Simulium striatum species-group of the subgenus Simulium, is closely related to S. (S.) argyrocinctum De Meijere from Java and Sumatra, but it is distinguished from the latter species by the smaller number of the male enlarged upper-eye facets and larval abdomen lacking dorsal pairs of conical protuberances. The distribution record of S. (S.) upikae Takaoka & Davies from Flores is corrected as that of S. (S.) eximium De Meijere. Some aberrant characters of the pupal gill filaments of S. (G.) atratum De Meijere, S. (G.) floresense Takaoka, Hadi & Sigit and S. (G.) sunapii Takaoka, Sofian-Azirun & Suana are illustrated. Characteristics of the fauna of black flies in this archipelago are briefly noted. Keys to all 19 species are provided for females, males, pupae and larvae.

Contribution to the fauna of blackflies of the tribe Simuliini (Diptera: Simuliidae) of the Sakha Republic (Yakutia)

The data on the fauna of blackflies of the tribe Simuliini (Diptera: Simuliidae) of the Sakha Republic (Yakutia) comprising the available literature, collections of the Zoological Institute of the Russian Academy of Sciences, and new material collected in Yakutia were analyzed. Three new species were recorded in the fauna of Yakutia: Archesimulium polare Rubzov, 1940, A. splendidum Rubzov, 1940, and A. tumulosum Rubzov, 1956. Distribution of 11 species was clarified. Processing of the collected material confirmed the presence of 9 blackfly species previously known from Yakutia. On the whole, the blackfly fauna of the region comprises 27 species.

Blackflies (Diptera: Simuliidae) in Croatia: species richness, distribution and relationship to surrounding countries.

All records of blackflies (Simuliidae) from the territory of Croatia are summarized, including previously unpublished data. The blackfly fauna of Croatia consists of 28 species. Simulium (Nevermannia) angustitarse (Lundström), Simulium (Nevermannia) cryophilum (Rubtsov) complex, Simulium (Nevermannia) lundstromi (Enderlein), Simulium (Nevermannia) vernum Macquart complex, Simulium (Simulium) argyreatum Meigen, Simulium (Simulium) bezzii (Corti) complex, Simulium paraequinum Puri and Simulium pseudequinum Séguy are reported for the first time from Croatia. Information related to the ecoregions, in which species were found and specific species traits are given. Genus Prosimulium Roubaud is represented by one species only. Genus Simulium Latreille is represented by 27 species in six subgenera, with subgenus Simulium Latreille s. str. being most species rich (13 species) and subgenera Boophthora Enderlein and Trichodagmia Enderlein represented each by only one species. Compared to the neighboring countries, the Croatian species assemblage is most similar to the fauna of Slovenia and least similar to that of Italy. The relatively low number of species, presence of several species complexes and unclear identity of other species show that further research of blackflies in Croatia is needed.

Contaminación del agua y distribución de la mosca negra (Diptera: Simuliidae) en el bosque Atlántico, Brasil

Black flies have medical importance because some species are vectors of the unenocerciasis and Mansonelosis, nevertheless, their ecology and potential use as bioindicators is still poorly studied in the Neotropical Region. In Brazil, bioindicators use is strongly focused in a multimetrical ecological index approach; this way, we investigated the black fly spatial distribution, in relation to abiotic factors correlated to water quality, to provide baseline information for their utilization as standalone indicators of lotic systems integrity. We have tested the hypothesis that environmental changes related to urbanization, lead to decreased abundance and loss in the number of species of the black fly fauna. The sampling was conducted in 10 urban and 10 preserved streams during the dry season (August-September) of 2012, in the mountainous region of Teres6polis, State of Rio de Janeiro, Brazil. The streams were characterized for their environmental integrity conditions and physico-chemical properties of water. In each stream, five different rapid points were sampled in a section of 50 meters, 10 meters apart from each other. The black flies were sampled with a kick-net sampler on rocky substrates. The material was separated and the larvae were sorted in morphotypes, and later, the final instar specimens were dissected and identified with the help of taxonomical literature at species level. A total abundance of 488 larvae from nine species were collected, 5 (1.02 %) in extremely impacted streams, 470 (96.31 %) in intermediate streams and 13 (2.66 %) in preserved streams. The visual evaluation (HII) differed in relation to the water physico-chemical evaluation, in which more variation in the characterization of the sampling sites was observed. In Canonical Correspondence Analysis Simulium subpallidum, S. inscrustatum and S. pertinax were significantly associated with intermediate values of most of the variables, and then to intermediate impacted sites. On the other hand, Lutzsimulium hirticosta, S. subnigrzm and Simulium sp. A were associated to low values of chemical variables, and then to more preserved sites. Most studies on the bioindicator potential of Simuliidae have suggested an approach to agricultural impacts, while our results, on the other hand, showed that Simuliidae species were present in streams with intermediate urban pollution impacts, but absent in heavily impacted sites. Thus, our data suggested that some species are associated to more pristine breeding sites, such as L. hirticosta and Simulium sp. A, while others may be good bioindicators of moderately impacted streams, such as S. nertinax. S. subnigrum and S. subpallidum.

New records of the black fly fauna (Diptera: Simuliidae) in two rivers of the western Amazonia, Brazil

Our goal was to verify the presence and the composition of black floy species in the Antimari and Acre rivers, located in the end of Western Amazonia, where this entomological inquiry was accomplished. Along the Antimari and Acre rivers, 19 localities were studied and 133 larvae and 197 pupae were collected, totalizing 330 black floy immatures. The following species are recorded for the first time in this area: Simulium (Psaroniocompsa) amazonicum Goeldi, Simulium (Psaroniocompsa) oyapockense Floch & Abonnenc, Simulium (Psaroniocompsa) quadrifidum Lutz, Simulium (Psilopelmia) lutzianum Pinto, Simulium (Psilopelmia) perfloavum Roubaud and Simulium (Notolepria) exiguum Roubaud. Our data suggest that S. amazonicum, S. oyapockense and S. quadrifidum have a wide geographical distribution as well as population density.

Contribution to the Knowledge of the Blackfly Fauna (Diptera, Simuliidae) of Estonia, Latvia and Lithuania

Data currently available on the distribution and associations of blackfly species in the Baltic States, with the exception of those for Lithuania, are scarce. Samples were collected in Estonia, Latvia and Lithuania from all types of running waters in order to contribute to the knowledge of the blackfly fauna of these countries. A total of 21 species were found within the study area, the most abundant ones being Simulium vernum Macquart, 1826 and S. cryophilum (Rubzov, 1959). Three species, Prosimulium ferrugineum (Wahlberg, 1844), P. hirtipes (Fries, 1824) and P. rufipes (Meigen, 1830) were recorded for the Baltic States for the first time. The cluster analysis shows three distinguishable groups of blackfly associations, correlated to three major habitat types – small creeks, midsize brooks and large rivers.

The fauna and distribution of black flies (Diptera, Simuliidae) in rivers of the south-eastern Altai Mountains

The spatial and typological structure of black fly populations were studied in different landscapes under different hydrological conditions at altitudes of 1000 to 2100 m. The effects of altitude, water temperature, flow velocity, granulometric composition of ground, and size of rivers are assessed. The black fly associations appeared to be most diverse in the upper and lower sections of the longitudinal profiles of the rivers studied. Transit species comprised the main part of the black fly associations.

Genetic variation tracks ecological segregation in Pacific island black flies

Geographic isolation is widely viewed as a key component of insular radiations on islands. However, strong ecological affinities may also reinforce isolation and promote genetic divergence. The black fly fauna in the Society Islands French Polynesia is notable for the number of closely related endemic species (31), and the morphological and habitat diversity of the larvae. Here, we measure ecological and morphological differences within and between two closely related species, Simulium oviceps and Simulium dussertorum and relate these differences to genetic distance. Phylogenetic analyses of a 920 bp fragment of the cytochrome oxidase I (COI) gene revealed a well-supported, ecologically divergent S. oviceps clade (larvae found in rivers instead of cascades) that shows little morphological differentiation. For both S. oviceps and S. dussertorum, genetic distance among populations is related to larval habitat, with cascade populations showing greater isolation from each other than river populations. Our data support the hypothesis that larval ecological shifts have played a role in the radiation of this black fly fauna.

Assessing morphological degradation in running waters using Blackfly communities (Diptera, Simuliidae): Can habitat quality be predicted from land use?

Based on benthic macroinvertebrate samples from 57 sites of streams in the European Central Highlands (Ecoregion 9; Illies (1978). Limnofauna Europaea, Stuttgart), the composition and the abundance of Simuliid species were analysed in relation to hydromorphological and land use parameters. Sampling sites were located at two stream types differing in catchment geology, stream morphology and channel width. Land use data were taken from the official German information system for cartography and topography (ATKIS) and the German River Habitat Survey. Land use categories suitable to describe the sampling sites were ‘agricultural land’, ‘urban’ and near-natural areas. Hydromorphological parameters of the sites were recorded using the AQEM site protocol (AQEM Consortium (2002). Manual for the application of the AQEM system. A comprehensive method to assess European streams using benthic macroinvertebrates, developed for the purpose of the Water Framework Directive. EVK1-CT1999-00027, Version 1.0. Available via the Internet from www.aqem.de). Relevant parameters controlling Simuliid distribution in streams were the mineral substrates and the biotic microhabitats. Blackfly larvae and pupae were sampled at each site for 15 min. Statistical analysis was performed by CANOCO ® (Ter Braak & Smilauer (1997). CANOCO Version 4.5. Biometrics Plant Research International, Wageningen, The Netherlands) using redundancy analysis (RDA). Our results show a stream type-specific composition of the Blackfly fauna. The analyses reveal sensitivity of the Simuliid species to morphological degradation, which is indicated by the shift in the longitudinal zonation of the Simuliid communities. Especially, Prosimulium hirtipes (Fries, 1824) and Simulium argyreatum Meigen, 1838 are typical representatives of headwater streams. While they seem to indicate undisturbed conditions of this stream type and react sensitively to the degradation of stream habitats, Simulium ornatum Meigen, 1818 and Simulium equinum (Linnaeus, 1758) are more tolerant to stream degradation. On catchment scale, ‘% natural forest’ and ‘% agricultural land use’ illustrate the degree of degradation of the two selected stream types. ‘Average stream depth’ and ‘relation riffles/pools’ account for hydromorphological degradation reflected by Simuliid species on the smaller reach scale. The analysis of habitat quality revealed that ‘% woody debris’ represents an important parameter of morphological degradation reflected by the Blackfly community.

The Simuliidae (Diptera) of the River Oder and its tributaries, with special reference to the re-appearance of Simulium (Schoenbaueria) nigrum (Meigen) in larger rivers in Central Europe

An investigation was carried out over a five-year period into the blackfly fauna of one of the most important rivers of the North-Central European lowlands, the Oder, together with its major tributaries (Nysa, Stobrawa, Oława, Barycz, Bobr, Kroisa, Lausitzer Neisse, Warta, Notec), from its source in the Oder Hills to its mouth in the Baltic Sea. Thirteen species were found. The two dominant indicator species, Simulium (Simulium) reptans (Linnaeus) and Simulium (Schoenbaueria) nigrum (Meigen), develop populations of plague proportions within this catchment area. Blackflies of the subgenus Schoenbaueria Enderlein have not been found in Germany or Poland for more than 70 years. However, since 1997, the species S. (Sch.) nigrum was found in the River Oder in enormous numbers together with S. (S.) reptans, and in the vicinity of their breeding sites in Poland and eastern Germany both species are serious pests of man and grazing animals. Notes are given on the biology, distribution and habitat preferences of S. (Sch.) nigrum. The paper concludes with a comparison of the River Oder with some other Central European rivers in respect of its blackfly fauna, together with data on the phenology of the two indicator species.

Do the Austrian blackfly fauna (Diptera: Simuliidae) support the typological approach of the EU water framework directive?

The Austrian blackfly fauna were analysed with regard to spatial (ecoregions, bioregions), vertical (altitude classes) and longitudinal zonation characteristics [stream order, biocoenotic (= fish) regions] on the basis of 2600 investigation sites. Of a total of 45 species recorded, Simulium ornatum, S. variegatum, S. argyreatum and S. reptans are the most common and most frequently distributed species, occurring in 60% of the investigated sites. Although the Austrian blackfly fauna seem to be quite well documented (on average one investigation site per 32 km 2) the jackknife analyses indicates that there are still some fauna deficits. Whereas the species diversity of the main ecoregions is quite similar, the number of species differs clearly between the bioregions. Within the typological context of the Water Framework Directive, the Austrian Simuliidae confirm the bioregions as the most useful spatial units for river typology. A further optimisation in predicting a target list of blackfly species of a site can be achieved by subdividing the bioregions into either catchment area and altitude classes or into longitudinal zonation types (biocoenotic regions).

A taxonomic account of the blackfly fauna of Iraq and Iran, including keys for species identification (Diptera: Simuliidae)

An account is given of what is so far known of the Simuliidae (blackflies) of Iraq and Iran with the object of providing a baseline for studies on this family of Diptera in both countries. A total of 1002 specimens from the area have been studied and 17 species identified in the material. Illustrated keys are given for the recognition of these species in larval, pupal and adult life stages. The composition and affinities of the simuliid fauna of Iraq-Iran are briefly discussed and a synoptic list is provided for the Simuliidae of the Middle East in general. A lectotype is designated for Simulium (Eusimulium) brachyantherum (Rubtsov) and first descriptions of the male of Simulium (Byssodon) buxtoni Austen and the pupa of Simulium (Nevermannia) brevitarse (Rubtsov) are included.

Studies on the ecology of blackflies (Diptera : Simuliidae) 44. Fauna of the blackflies in Okayama Prefecture in spring

The fauna and distribution of blackflies in Okayama Prefecture, Japan, were examined based on the collections of larvae and pupae from streams and adults flying around the collectors at 74 sites in spring of 1991. A total of 4,949 blackflies, [3,675 larvae (74.3% in total number), 1,259 pupae (25.4%) and 15 adults (0.3%)], were collected at 72 sites. These blackflies were classified into 23 species of three genera : Prosimulium Roubaud, Cnephia Enderlein and Simulium Latreille. Nine species, i.e. Prosimulium (Prosimulium) kiotoense, P (P.) yezoense, Cnephia (Stegopterna) mutata, Simulium (Nevermannia) aureohirtum, S. (N) konoi, S. (N.) mie, S. (Gomphostilbia) ogatai, S. (Simulium) japonicum and S. (S.) kawamurae were newly recorded from Okayama Prefecture. Further, five undescribed and / or unidentified spcies, e.g. E. sp. 2, S. sp. H, S. sp. O-1, S. sp. O-2 and S. (Helichiella) sp., were recognized. The most abundant species was S. (N) uchidai (22.9% in total number), followed by S. (S.) arakawae (19.7%), S. (S.) japonicum (18.8%), P. (P.) kiotoense (10.9%), S. (N) subcostatum (8.1%), S. (S.) suzukii (3.6%) and S. (Gnus) bidentatum (3.3%). The most widely distributed species was S. (N) uchidai (55 sites), followed by S. (S.) japonicum (44 sites), S. (N) subcostatum (32 sites), S. (S.) arakawae (26 sites), S. (Gn.) bidentatum (26 sites), S. (S.) rufibasis (22 sites), S. (O.) aokii (17 sites) and P. (P.) kiotoense (14 sites).

14. 香川県における夏期のブユ採集成績(第53回日本衛生動物学会東日本支部大会講演要旨)

14. 香川県における夏期のブユ採集成績(第53回日本衛生動物学会東日本支部大会講演要旨)