Bivalve Shells Research Articles

First record of shell-boring polychaetes in Pacific oyster (Magallana gigas) seed

Shell-boring polychaetes such as Polydora websteri have eroded the value of aquaculture operations worldwide. These pests burrow into the shells of bivalves, creating blisters that are unappealing to consumers and that make oysters less valuable on the half-shell market. Our team has documented the impact of these polychaetes on Pacific oysters (Magallana gigas) of the US West Coast, a region that produces 38 % of the farmed shellfish in the US. In the course of our research, we also noticed the presence of these polychaetes in oyster seed. To assess the prevalence of shell-boring polychaetes in seed, we sampled Floating Upweller Systems (FLUPSYs) from Alaska (n seed = 50), Hawai'i (n seed = 150), and Washington (n seed = 400). Prevalence of infestation varied by state (Alaska = 10 %; Hawai'i = 33 %; Washington = 8.5 %), and ranged from 0 to 100 % across FLUPSYs. We found no evidence for larger seed (~5 cm) being more infested than smaller seed (~1 cm). These data confirm that shell-boring polychaete pests are present in oyster seed. We end by proposing ideas for control treatments that could be tested on seed to mitigate infestation by shell-boring polychaetes.

Stratigraphy and microfacies analysis of the Lower–Middle Jurassic platform carbonate succession in the Yeşilovacık (Mersin) area (Central Taurides, S Turkiye)

This study covers the detailed analyses of stratigraphy, microfacies and depositional environments of the Lower-Middle Jurassic carbonate succession in the southern part of Anatolide-Tauride Carbonate Platform in southern Türkiye (south of Central Taurides, around Yeşilovacık). The carbonate succession is composed of the Dibekli and Tokmar formations, and the Işıklıkızıtepe Member which forms the base of the Tokmar Formation. The Dibekli Formation (Upper Sinemurian-Lower Bajocian) is composed of limestones intercalated with intraformational conglomerates in the lower parts, with dolostones in the lower to middle parts and with oncoidal limestones in middle to upper parts. The Işıklıkızıtepe Member (Upper Bajocian) starts with basal conglomerates containing brown-red- iron nodules and consists of sandstone and sandy-clayey limestone alternations. These are overlain by thin limestone layers passing upward to thick-bedded dolostones of the Tokmar Formation (Upper Bajocian–Bathonian). The middle to upper parts of the formation are composed of sandy, oncoidal limestones rich in bivalve shells and end with dolostones-limestone alternations in the upper part. According to microfacies analyzes and litho-bio content; Late Sinemurian-Plesbachian is a period that low-to moderate-energy, restricted inner platform conditions dominated the depositional environments. The presence of comman intraformational conglomerates shows tectonic activity from time to time in this period. The Toarcian to the earliest Bajocian, low-energy intertidal and shallow lagoon conditions dominated the depositional environments, and in the Early Bajocian, the depositional environment changed to a high-energy sand shoal and above wave base environment. The basal conglomerate consisting of brown-red, iron-noduler and bauxite grains at the base of the earliest Late Bajocian indicates a short-term emersion and continued under low energy shallow lagoonal conditions during the Late Bajocian–Bathonian. Environmental changes during the Toarcian to Early Bajocian, and the earliest Late Bajocian short-term emersion, and the environmental changes during the Late Bajocian-Bathonian are interpreted to short-term sea level changes. Yeşilovacık Lower-Middle Jurassic carbonate sequences are correlated with those in peri-Mediterranean platforms. They show close similarities to the other Tauride carbonate sequences in terms of stratigraphy and facies. Paleontological and sedimentological analyses enable us to reconstruct a detailed dating and paleoenvironmental evolution for the Yeşilovacık carbonate deposits during the Lower-Middle Jurassic period.

Three New Species of Mytilinidioid Fungi (Dothideomycetes, Ascomycota) from Mexico.

Mytilinidioid fungi are conchiform in nature, with the appearance of bivalve shells or wedge-shaped, rigid, brittle, and carbonaceous hysterothecia growing on the bark of gymnosperms or angiosperms. Based on their morphological characteristics and molecular markers (ITS and LSU), this study describes three new species of mytilinidioid fungi: Ericboehmia mexicana of the family Hysteriaceae of the order Hysteriales and Lophium pinicola and Mytilinidion mexicanum of the family Mytilinidiaceae of the order Mytilinidiales. The first species grows on Liquidambar styracyphlua, the second species grows on Pinus patula, and the third species grows on Acacia californica subsp. pringlei. The specimens studied were deposited in the ENCB Herbarium.

Sustainable Stabilizer Derived from Calcium- and Phosphorus-Rich Biowaste for Remediation of Heavy Metal-Contaminated Soil: A Critical Review

Soil pollution by heavy metals (HMs) is a major environmental problem around the world. The addition of biowaste-based stabilizers for HM remediation has recently gained attention due to its relatively low cost and eco-risk, abundance, ease of operation, and quick remediation results. Among these stabilizers, shell (crustacean shell, bivalve shell, and eggshell), starfish, and bone-based stabilizers are particularly attractive because of their high Ca and P contents, allowing for highly efficient HM immobilization and simultaneous supplement of nutrients to the soil. However, a comprehensive review focusing on these stabilizers is currently missing. Therefore, this review attempts to summarize the HM immobilization efficiency of these stabilizers and the mechanisms associated with HM stabilization, and perform an operation cost estimation and cost comparison. Cost comparisons among different stabilizers are widely ignored in reviews due to the lack of reliable cost estimation tools or methods. However, for practical application in soil remediation, cost is one of the most important factors to consider. Thus, a simple but reasonable cost estimation method is developed and discussed in this review. Bivalve shell-based stabilizers demonstrated the most promising results for the immobilization of soil HMs in terms of higher performance and lower cost. Current research limitations, challenges, and recommendations regarding possible future research directions are also provided.

Mechanical Characteristics of Bivalve (Mollusc) Shells from the East Coast of India: Implications and Their Significance on the Survival Strategy

Due to natural selection, an increase in biotic and abiotic factors develops an adaptive response in bivalves (molluscs) for survival. Morphological variation in a bivalve is strongly correlated with the changes in the structural and compositional properties of a shell. Therefore, the mechanical characteristics of a shell reflect its morpho-functional adaptation against predation and ecological stresses. An initial investigation into the primary mechanical attributes of predominant bivalve shells from the east coast of India, Tegillarca granosa, Meretrix meretrix, Sunetta meroe, Mactra turgida and Donax scortum, has been documented in this study. The quantitative assessment of the centreline thickness, Vickers hardness and density variation of the shells were measured and correlated along with the preliminary investigation of the microstructural patterns. The results from our study are in agreement with the most vulnerable region of a shell, located just below the umbonal zone, which has been verified through predatory boreholes in other studies. Our data shows a high correlation between shell hardness and density, which could also be the potential properties to consider in detail for future studies, apart from the shell thickness, which could provide a broader perspective on the stability of bivalve shells. Different microstructural patterns observed in the species could be phylogenetically driven based on their life modes.

Bivalve shell growth from molecular to sclerochronological scale: Environment and intrinsic factors control increment deposition

Biomineralisation of bivalve shells raises questions at the level of genes to the final calcified product. For the first time, gene expression has been studied in association with growth increment deposition in the mussel Mytilus galloprovincialis. A short-term experiment highlighted that biomineralisation genes exhibit a rhythm of expression consistent with the observed tidal increment formation. Long-term mark-recapture experiments were conducted in three Mediterranean environments and revealed that the mussel shells harbour complex incrementation regimes, consisting of daily, tidal and a mixed periodicity of ∼1.7 growth increment.d−1 formed. The latter is likely related to the local tidal regime, although the mussels were continuously submerged and exposed to a small tidal range. The pattern of growth increments shifted from mixed to daily in Mediterranean lagoon, and to tidal at sea, probably linked to biological clocks. Based on our results and the literature, a hypothetical model for mussel shell increment formation in various habitats is proposed.

Controls on the Barium and Strontium Isotopic Records of Water Chemistry Preserved in Freshwater Bivalve Shells.

Biogenic carbonates, including bivalve shells, record past environmental conditions, but their interpretation requires understanding environmental and biological factors that affect trace metal uptake. We examined stable barium (δ138Ba) and radiogenic strontium (87Sr/86Sr) isotope ratios in the aragonite shells of four native freshwater mussel species and two invasive species in five streams and assessed the effects of species identity, growth rate, and river water chemistry on shell isotopic composition. Shells were robust proxies for Sr, accurately reflecting 87Sr/86Sr ratios of river water, regardless of species or growth rate. In contrast, shell δ138Ba values, apart from invasive Corbicula fluminea, departed widely from those of river water and varied according to species and growth rate. Apparent fractionation between river water and the shell (Δ138Bashell-water) reached -0.86‰, the greatest offset observed for carbonate minerals. The shell deposited during slow growth periods was more enriched in lighter Ba isotopes than the rapidly deposited shell; thus, this phenomenon cannot be explained by aragonite precipitation kinetics. Instead, biological ion transport processes linked to growth rate may be largely responsible for Ba isotope variation. Our results provide information necessary to interpret water chemistry records preserved in shells and provide insights into biomineralization processes and bivalve biochemistry.

Stable carbon and oxygen isotope-based sclerochronology of bivalve mollusk shells from the Upper Cretaceous (upper Campanian) Coon Creek Formation in Tennessee, USA: Implications for paleoecology and paleoenvironment

Stable carbon and oxygen isotope-based sclerochronology was carried out on well-preserved fossil shells of bivalve mollusks Crassatella vadosa and Cucullaea vulgaris from the Upper Cretaceous (upper Campanian) Coon Creek Formation in Tennessee, USA, to reconstruct their life-history traits and sedimentary settings on an intra-annual time scale. The δ13CShell values along with the shell growth showed the cyclic variation with downward convex to lower value, likely reflecting the seasonal cycle of ambient seawater dissolved inorganic carbon (DIC) or metabolism of the bivalve or both. The δ13CShell cycles were largely associated with the strong growth disturbance lines, supporting hypothesis that the growth lines were formed annually. We estimated the ages of the shells based on both δ13C cycle or the growth cessation line. The modeled relationship between age and shell growth showed that Cr. vadosa and Cu. vulgaris grew up to about 40 mm at 7 years old and about 65 mm at 10 years old, respectively. The paleotemperatures estimated from the δ18OShell profiles of the examined specimens ranged from 16.2 °C to 24.2 °C and from 18.8 °C to 27.0 °C when assuming the bottom seawater δ18O to be −1.00‰ and −0.46‰ (VSMOW), respectively, and showed no clear seasonal cycle. The bottom seawater temperatures reconstructed from the specimens of the two bivalve species were lower than the near-surface seawater temperatures (29.0–30.6 °C) estimated from the planktic foraminiferal δ18O. We inferred that the benthic bivalve habitat of the Coon Creek Formation was at least 10 m or deeper below the thermocline.

Significance of microstructural defect-tolerant in the battle for survival: Mantis shrimp VS. Nacre

Mantis shrimps use their robust claws to repeatedly strike and break the shells of bivalves during predation without sustaining damage themselves. It appears to be a confrontation between the bouligand microstructure and the brick-and-mortar microstructure of the nacre layer. In the daily struggle for survival, defects are inevitably introduced. To elucidate the mechanical mechanisms exhibited by the two microstructures in the struggle for survival, thus a bold conjecture was proposed: the bouligand structure exhibits less sensitivity to random defects compared to the brick-and-mortar structure, enabling mantis shrimps to survive in the battle for existence. To verify this hypothesis, numerical models of bouligand and brick-and-mortar microstructures were established considering interfacial random defects. The results indicated that for various defect volume fraction, the bouligand structure demonstrates superior mechanical performance compared to the brick-and-mortar structure. In terms of peak load and damage dissipation energy, the bouligand structure exhibits higher tolerance to interfacial random defects than the brick-and-mortar structure, delaying the occurrence of damage in the Bouligand structure while prematurely inducing damage in the brick-and-mortar structure. This study not only reveals the mechanical mechanisms behind the advantages of biological microstructures in the struggle for survival but also provides technical references for future biomimetic microstructure designs.

Cryptic fauna in abandoned bivalve shells and taphonomy of bivalve steinkerns in the Late Ordovician of Baltica

ABSTRACT The encrusted bivalve steinkerns in the Upper Ordovician of Estonia suggest that the rapid sea-floor cementation facilitated the early lithification of the sediment within the bivalve shell and the shell valves, likely aragonitic in composition, were dissolved due to calcite sea conditions similarly to many other regions in the Ordovician. The abandoned bivalve shells contained cryptic invertebrates. The coelobite fauna was dominated by encrusting brachiopods, followed by bryozoans and cornulitids. The bryozoans and cornulitids do not prefer specific regions of the shell interior, indicating that the waters within the shell were sufficiently nutrient-rich throughout. Encrusting brachiopods are always confined to valve margins, suggesting that the waters were nutrient-rich enough for the brachiopods only at the edge of the outer environment. Thus, it is possible that bryozoans and cornulitids were better adapted for a cryptic life than the encrusting brachiopods in the Late Ordovician of Estonia.

Morphology, Biochemistry and Distribution of Villorita cyprinoides and Meretrix casta (Bivalve) Shells in Vembanad Estuary, Kerala, India

Estuaries are among the most commercially significant ecosystems on the earth, and they provide different habitats for numerous bivalve species. The Clams are one of the most widely distributed and used aquatic bivalves, providing much more protein-rich food than mussels and oysters. The Vembanad Estuary is one of the richest clam fisheries coastal wetland in Kerala. The present study focusing on comparatives ofmorphology and biochemistry of Villorita cyprinoides and Meretrix casta shells in Vembanad Estuary. It also attempts to understand the variations in clam distribution caused by salinity fluctuations and sediment texture. According to the morphological and morphometric analysis, medium size shells were found in greater abundance in both species. Atotal of 306 shells of V. cyprinoides and 169 shells of M. casta were obtained from the ten sample locations of Vembanad Estuary. The density of V. cyprinoides shells (192) was higher in the southern part while the northern portion of Vembanad Estuary represented by M. casta shells (108) with comparatively lower density. V. cyprinoides has a negative linear correlation with salinity, as indicated by R2 of 0.96. M. casta, on the other hand, exhibits a positive correlation with salinity, with 0.94 linear coefficient. V. cyprinoides was more prevalent in clayey and silty sediments, while M. casta was more common in sandy sediments. The X-ray Fluorescence (XRF) analysis showed that the Calcium Oxide percentage of V. cyprinoides and M. casta shells were 39.47% and 38.72%, respectively, while all other metal oxides were present only in trace amount. Keywords: Bivalves, Villorita cyprinoides, Meretrix casta, Major Oxides, Vembanad Estuary

Depositional model of the Holocene coquinas - Albardão platform, southern Brazil

For the past decade, giant deepwater oil discoveries in the pre-salt section of the Campos and Santos basins of Brazil, have brought significant attention to offshore exploration activities along the South Atlantic margins. The prolific Cretaceous coquina deposits in these basins are part of the pre-salt rock record and constitute an effective but complex and heterogeneous hydrocarbon reservoir difficult to predict and model. Parting from this context, an evaluation of the sedimentological, structural and taphonomic criteria for coquinas are essential to better understand and predict the facies distribution and depositional models of the pre-salt coquinas strata. Based on this premise, the present work aims to genetically interpret 133 mixed carbonate-siliciclastic bottom sediments of the Albardão shelf – a modern marine coquina analogue, using facies description, investigating the relationship with hydrodynamic forces, and accessing the influence of morphology and structural framework on their deposition. From these analyses, we recognized a hybrid facies, three modern carbonate facies in analogy to the carbonate rock classification and four siliciclastic facies. These eight facies were then grouped into three facies associations representing high, moderate, and low energy facies. The high energy facies association comprises rudstones (Rf) and grainstones (Gf) with highly fragmented bivalve shells and barnacles abundantly present in the beach system, above the fair-weather wave base limit (FWWB). These facies also occur offshore on bathymetric highs above the storm wave base limit (SWB) but display less reworking than the coastal high energy facies above the FWWB due to wave shoaling. The moderate energy facies association consists of hybrid sand (Hs), sand (S) and muddy sand (mS) occurring between the FWWB and SWB limits in the offshore transition zone with extensive winnowing action and low rate of reworking. The low energy facies association includes sandy mud (sM), mud (M) and micritic mud (Mc), characterized by the decantation of the fine sediments below the offshore SWB limit. The results confirm a bottom facies distribution controlled by depth, shelf profile morphology and energy from incident waves. The fragmented rudstone and fragmented grainstone facies are the best-recognized reservoirs with both having high porosity and high permeability.

Ancestral and contemporary intertidal mariculture practices support marine biodiversity in the northeast Pacific

The cultivation of seafood now outpaces extraction from wild populations. This novel state in the history of human-marine ecosystem interactions highlights the importance of identifying cultivation practices that balance production and conservation. Here, we test the hypothesis that two shellfish cultivation practices, one that spans decades and the other millennia, bolsters habitat complexity, which increases epifaunal (surface) invertebrate diversity. To do so, we conducted multiple surveys of 24 First Nations clam gardens, shellfish aquaculture farms, and non-walled or unmodified beaches along the west coast of Canada. We show that habitat alterations specific to each cultivation practice restructure epifaunal communities at several ecological and spatial scales. Distinct communities within clam gardens and shellfish farms are a function of habitat complexity at 25–50 and 50–100 cm resolutions and changes in the amount of gravel, bivalve shells, and seaweeds. Our findings highlight how resource cultivation can contribute to achieving sustainable human-ecosystem interactions.

Isotopic and mineralogic bias introduced by pulverization of aragonite

RationaleStable carbon and oxygen isotope data of biogenic and abiogenic aragonite are of fundamental relevance in paleoclimate research. Wet‐chemical analysis of such materials requires well‐homogenized, fine‐grained powder. In the present study, the effect of different grinding/milling methods on sample homogeneity and the potential risk of unintentional calcite formation and isotope shift were evaluated.MethodsShells of Arctica islandica and aragonite sputnik crystals were pulverized using a set of commonly used methods, including a hand‐held drill, a vibromill operated at various settings (with and without liquid nitrogen cooling, changes in ball diameters, frequencies, and processing durations), and an agate mortar and pestle. Stable isotope values were measured using an isotope ratio mass spectrometer operated in continuous flow mode. Identification of mineral phases was obtained by powder X‐ray diffraction (PXRD), Raman spectroscopy, and attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR) spectroscopy. Calcite content was quantified by PXRD Rietveld refinement.ResultsSamples showed substantial homogeneity, in particular after vibromilling (duration 3–10 min). More vigorous grinding resulted in larger fractions of calcite (0.5–4.2 wt%) and a concomitant δ18O and δ13C decrease, specifically in bivalve shells. The only method for producing pure aragonite powder was by pounding the aragonite sputniks manually with an agate mortar and pestle.ConclusionsNone of the studied, commonly used machine‐based pulverization methods produced pure aragonite powder from samples consisting originally of aragonite. These findings have significant implications for light‐stable isotope‐based paleoclimate reconstructions. Except for abiogenic aragonite powder produced by pounding in an agate mortar, paleotemperatures would be overestimated.

Microscale analysis reveals nature of hydrophobic biopolymers in aragonite bivalve shells with crossed-lamellar architecture

Bivalve shells with crossed-lamellar architecture consist of random orientation of anisotropic crystallographic directions that adds to the optimization of the mechanical properties of shell biominerals. The source of these anisotropic distortions has been attributed to the organic macromolecules in the inorganic unit. However, very little is known about the organic macromolecules in shells with crossed-lamellar architecture because it is believed that the organics in this shell ultrastructure can hardly be characterized owing to its low content, about 1 wt%. Here, the shells of two bivalve species (Callista kingii and Anadara trapezia) with crossed-lamellar architecture, have been studied by microRaman spectroscopy, FTIR, and histochemical analysis. For the first time, organic macromolecules and inorganic components were simultaneously identified. Using spectroscopic techniques (microRaman and FTIR) and a confocal scanning fluorescence microscopy, the results herein demonstrated minor chitin in the studied samples and document structural protein, collagen-like matrix as the major organic component. The SEM observations revealed a morphological biopolymer meshwork composed of a polygonal network. Based on those combined evidence, this study suggests that the interactions between polysaccharide-based biomolecules and collagen-like matrix are crucial for the mechanical properties of crossed-lamellar layers in bivalve shells. This study highlights the importance of using in-situ approaches for these types of biomaterials and provides another source of biomaterials.

Incipient Motion of Single Shells under Currents in Flume Experiments

Understanding the motion thresholds of shells is important, as shell motion allows the analysis of beach profiles, prevents excessive erosion of the coastline, and helps to resource the use of discarded shells, providing new ideas for the protection of beaches. In this study, the orientational motions and motion thresholds of two types of typical molluscan shells, bivalve and gastropod shells, were investigated by means of flume experiments. The final orientations with the statistically highest number of occurrences during the orientational motions of each shell were used as the initial orientations for the respective threshold flow velocity measurements. The critical Shields parameter and the incipient mean velocity of the flow were used to represent the critical threshold of the motion. The critical Shields parameters for bivalve shells in the convex upward position were overall higher on average than those for gastropod shells. The experimental data showed that the incipient mean flow velocities of bivalve shells in the convex upward position were about 1.4–2.8 times larger than those in the convex downward position. The incipient mean velocity data were regressed to obtain the motion threshold equations applicable to bivalve shells in the convex upward and convex downward positions as well as gastropod shells under different final orientations.

Preserved appendages in a Silurian binodicope: implications for the evolutionary history of ostracod crustaceans.

Ostracod crustaceans originated at least 500 Ma ago. Their tiny bivalved shells represent the most species-abundant fossil arthropods, and ostracods are omnipresent in a wide array of freshwater and marine environments today and in the past. Derima paparme gen. et sp. nov. from the Herefordshire Silurian Lagerstätte (~430 Ma) in the Welsh Borderland, UK, is one of only a handful of exceptionally preserved ostracods (with soft parts as well as the shell) known from the Palaeozoic. A male specimen provides the first evidence of the appendages of Binodicopina, a major group of Palaeozoic ostracods comprising some 135 Ordovician to Permian genera. The appendage morphology of D. paparme, but not its shell, indicates that binodicopes belong to Podocopa. The discovery that the soft-part morphology of binodicopes allies them with podocopes affirms that using the shell alone is an unreliable basis for classifying certain fossil ostracods, and knowledge of soft-part morphology is critical for the task. Current assignment of many fossil ostracods to higher taxa, and therefore the evolutionary history of the group, may require reconsideration.

Late Miocene mollusks from the Dar Pahn Unit, Makran, SE Iran

Diverse, abundant and well-preserved mollusk fauna are here reported for the first time from the eastern coast Makram area, where Upper Miocene (Tortonian) sediments of the Dar Pahn Unit crop out, representing shoreface tidal influenced deposits composed mainly of ligh-gray marls. Multiple articulated bivalves and completely preserved the gastropod and bivalve shells indicate little or no transport at all from the original habitat of the mollusk assemblage. A total of 313 specimens, from which 271 correspond to gastropods, were identified only at generic level, since the purpose of this contribution is to offer an introduction to this exceptionally well-preserved mollusk assemblage. Previous studies of the area include detailed nannoplankton biostratigraphy and the report of a balaenopterid cetacean. Future studies include the detailed identification of the specimens, along with sediment analysis in order to find protoconchs and offer an extended version of this introductory report.

Bioavailability, risk assessment, and source traceability of potentially toxic elements in bivalve species widely consumed in the emblematic Acapulco Bay, Mexico

The concentration of potentially toxic elements (PTE) was determined in edible tissue, digestive system, and shells of two bivalve species (Hyotissa hyotis and Spondylus limbatus) highly consumed in Acapulco Bay (southern Mexico) to evaluate the impact on marine ecosystems and human health risk associated with the consumption of potentially contaminated bivalves. PTE concentrations (Ba–Cd–Cu–Co–Cr–Mn–Ni–Pb–Sr–V–Zn) in edible tissues were lower than in the digestive system and shells in both bivalve species. Except for Cr, Pb, and Cd, PTE concentrations in the edible tissue of both species did not exceed international threshold limits. In addition, the biota-sediment accumulation factor values indicate bioaccumulation in edible tissues (Ni, Cd, Pb, Co, and Zn), digestive system (V, Ni, Cu, Cd, Pb, Co, and Zn), and shells (V, Cr, Pb, Co, and Mn), evidencing availability of pollutants in the Acapulco Bay. Contents of Pb and Cd found in edible tissue can threaten human health. Target Hazard Quotient values indicate that the consumption of edible tissue does not represent any risk of developing non-cancerogenic effects. However, the Target Cancer Risk values indicate a moderate risk of developing carcinogenic effects due to the accumulation of Cr and Pb and an even greater risk due to the accumulation of Cd. Additionally, although local rocks and sediments should be the only source of PTE in uncontaminated marine ecosystems, a low radiogenic Pb isotope composition was detected in the bivalve shells, which partially overlap with Pb isotope compositions of paints and gasoline, indicating anthropogenic lead sources participation in the Acapulco Bay.

An invasive bivalve with the potential to reconstruct chronologies of geomarkers in a large South American basin.

The Sr/Ca and 87Sr/86Sr ratios are used as natural indicators (geomarkers) in fish to reveal migratory patterns, due to significant relationships between these ratios measured in the water and in the fish calcified structures (otoliths, bones). The aim of this study was to assess the potential use of the Limnoperna fortunei shell as a proxy for monitoring the spatial and temporal variability of Sr/Ca and 87Sr/86Sr in water. These ratios were compared in water samples and bivalve shells proceeding from eight sites of four hydrogeological regions of the La Plata Basin (Argentina and Uruguay), collected in two hydrological periods (winter and summer) in order to depict the pattern of geographical and temporal variability and to evaluate the relationship between both matrices. Significant positive linear relationships were found between shell edge and water for 87Sr/86Sr (R2 = 0.96-0.97, p < 0.005) and Sr/Ca (R2 = 0.83-0.86, p < 0.05) in summer and winter. Slopes did not differ significantly from 1 (p > 0.05) for 87Sr/86Sr, while they differed from 1 for Sr/Ca in both seasons (p < 0.05), indicating that the elemental ratio would be influenced by endogenous/exogenous factors. No significant seasonal differences were found for both natural tags (p > 0.05), except for the lower Uruguay River (87Sr/86Sr) and outer estuary (Sr/Ca). These results indicate that the L. fortunei shell could be a valuable tool for monitoring spatio-temporal variations in water 87Sr/86Sr. Shell Sr/Ca shows high potential utility as a geomarker in freshwater, but additional assessments are needed to understand the impact of temperature and growth on this ratio.