Biotic Environmental Stresses Research Articles

Increased survival of honey bees consuming pollen and beebread is associated with elevated biomarkers of oxidative stress

IntroductionSignificant losses of honey bee colonies have been observed worldwide in recent decades. Inadequate nutrition is considered to be one of the factors that can reduce honey bee resistance to abiotic and biotic environmental stresses. Accordingly, we assessed the impact of food composition on worker bee survival.MethodsBees in cages were fed six different diets, and then their survival, levels of thiobarbituric acid reactive substances and protein carbonyl groups, catalase and lysozyme activities were evaluated.Results and DiscussionAfter 17 days of feeding, the lowest mortality was observed in the group of bees that received sucrose solution with the addition of willow pollen or artificial rapeseed beebread or artificial willow beebread (diets 4–6). The highest mortality was found in bees that consumed only sucrose solution (diet 1) or the sucrose solution supplemented with a mixture of amino acids (diet 2), which can be explained by the lack of vitamins and microelements in these diets. In the group of bees that received the sucrose solution with rapeseed pollen (diet 3), mortality was intermediate. To check whether the decrease in insect survival could be related to oxidative damage, we evaluated biomarkers of oxidative stress. Consumption of pollen (diets 3 and 5) and artificial beebread (diets 4 and 6) enhances protein carbonylation in worker bees. Feeding bees artificial beebread also resulted in increase in lipid peroxidation and catalase activity, which is probably due to the presence of hydrogen peroxide in the honey contained in beebread. Remarkably, the increase in biomarkers of oxidative stress was not accompanied by adverse but positive effects on insect survival. A lack of amino acids and proteins in the diet 1 did not cause oxidative stress, but led to an increase in lysozyme activity in hemolymph, a biomarker of immune system status. In conclusion, we believe that the increase in oxidative stress biomarkers we found do not indicate oxidative damage, but rather reflect the changes in redox balance due to consumption of certain dietary options.

Marine gastropods at higher trophic level show stronger tolerance to ocean acidification

Climate change and anthropogenic activities are producing a range of new selection pressures, both abiotic and biotic, on marine organisms. Although it is known that climate change can differentially affect fitness‐related traits at different trophic levels of the food web, it is not clear if different trophic levels will respond via phenotypic plasticity in the form of maintenance of phenotypes in the face of abiotic and biotic environmental stress similarly. To answer this question, we combined a mesocosm experiment (120 days) using a food web comprising three gastropod species from two trophic levels (grazers and meso‐predators) and a meta‐analysis including 38 studies to address whether different trophic levels exhibit similar phenotypic responses to abiotic and biotic variables. Abiotic (ocean acidification) and biotic (predation) stress significantly influenced body mass, shell mass, shell thickness and shell strength in both grazers and meso‐predators in the mesocosm experiment, with the magnitude of OA effects greater on the meso‐predator than the grazers; a result supported by the meta‐analysis. In contrast, both mesocosm experiment and meta‐analysis found that predation risk induced stronger responses in shell morphology for grazers compared to meso‐predators. Together, our findings indicate that higher trophic level species are better able to maintain aspects of their phenotype under OA, suggesting that they may show greater tolerance to climate change effects in general, while lower trophic levels express higher levels of plastic inducible defences to maintain function when under threat of predation. By using marine snails as a model, our study provides new knowledge for understanding how changing environmental conditions may alter biological interactions, and increases our understanding of how climate change may affect ecological communities in which gastropods play a key role.

Harnessing belowground processes for sustainable intensification of agricultural systems

Increasing food demand coupled with climate change pose a great challenge to agricultural systems. In this review we summarize recent advances in our knowledge of how plants, together with their associated microbiota, shape rhizosphere processes. We address (molecular) mechanisms operating at the plant–microbe-soil interface and aim to link this knowledge with actual and potential avenues for intensifying agricultural systems, while at the same time reducing irrigation water, fertilizer inputs and pesticide use. Combining in-depth knowledge about above and belowground plant traits will not only significantly advance our mechanistic understanding of involved processes but also allow for more informed decisions regarding agricultural practices and plant breeding. Including belowground plant-soil-microbe interactions in our breeding efforts will help to select crops resilient to abiotic and biotic environmental stresses and ultimately enable us to produce sufficient food in a more sustainable agriculture in the upcoming decades.

Stress, senescence, and specialized metabolites in bryophytes.

Life on land exposes plants to varied abiotic and biotic environmental stresses. These environmental drivers contributed to a large expansion of metabolic capabilities during land plant evolution and species diversification. In this review we summarize knowledge on how the specialized metabolite pathways of bryophytes may contribute to stress tolerance capabilities. Bryophytes are the non-tracheophyte land plant group (comprising the hornworts, liverworts, and mosses) and rapidly diversified following the colonization of land. Mosses and liverworts have as wide a distribution as flowering plants with regard to available environments, able to grow in polar regions through to hot desert landscapes. Yet in contrast to flowering plants, for which the biosynthetic pathways, transcriptional regulation, and compound function of stress tolerance-related metabolite pathways have been extensively characterized, it is only recently that similar data have become available for bryophytes. The bryophyte data are compared with those available for angiosperms, including examining how the differing plant forms of bryophytes and angiosperms may influence specialized metabolite diversity and function. The involvement of stress-induced specialized metabolites in senescence and nutrient response pathways is also discussed.

Structural and Functional Characterization at the Molecular Level of the MATE Gene Family in Wheat in Silico.

A series of multidrug extransporters known as the multidrug and potentially toxic extrusion (MATE) genes are found in all living things and are crucial for the removal of heavy metal ions, metalloids, exogenous xenobiotics, endogenous secondary metabolites, and other toxic substances from the cells. However, there has only been a small amount of them in silico analysis of the MATE family of genes in plant species. In the current study, the MATE gene family was characterized in silico where two families and seven subfamilies based on their evolutionary relationships were proposed. Plant breeders may use TraesCS1D02G030400, TraesCS4B02G244400, and TraesCS1A02G029900 genes for marker-assisted or transgenic breeding to develop novel cultivars since these genes have been hypothesized from protein-protein interaction study to play a critical role in the transport of toxic chemicals across cells. The exon number varies from 01 to 14. One exon has TraesCS1A02G188100, TraesCS5B02G562500, TraesCS6A02G256400, and TraesCS6D02G384300 genes, while 14 exons have only two genes that are TraesCS6A02G418800 and TraesCS6D02G407900. Biological stress (infestations of disease) affects the expression of most of the MATE genes, with the gene TraesCS5D02G355500 having the highest expression level in the wheat expression browser tool. Using the Grain interpretation search engine tool, it is found that the vast bulk of MATE genes are voiced throughout biotic environmental stresses caused by disease pests, with the genotype TraesCS5B02G326600.1 from family 1 exhibiting the greatest level of expression throughout Fusarium head blight infection by Fusarium graminearum after 4 days of infection. The researchers constructed 39 ternary plots, each with a distinct degree of expression under biotic and abiotic stress settings, and observed that 44% of the triplets have imbalanced outputs (extreme values) due to their higher tissue specificity and increased intensity.

Comprehensive assessment of Hordeum vulgare in the northern forest-steppe of the Tyumen region

Barley (Hordeum vulgare L.) occupies more than 80% of the sown area of forage crops. Barley is widely cultivated in many areas with contrasting soil and climatic conditions; breeding varieties that are resistant to abiotic and biotic environmental stress factors is an important factor that would make it possible to obtain grain with the required nutritional and forage qualities. The creation of barley varieties is a promising area of breeding in the world. To create varieties, it is necessary to consider the main directions: early ripening and high yield. To create an early ripening, high-yielding variety and hybrid of spring barley with high technological qualities of grain, the leading role belongs to the scientifically grounded selection of the source material. The article presents the results of a 3-year collection samples’ study from the world collection of the All-Russian Institute of Plants’ Genetic Resources named after N.I. Vavilov (VIR).

The influence of downy mildew tolerance of grape varieties on the biosynthesis of stilbenes in callus as potential sources of bioactive substances

Stilbenes represent a considerable practical interest in relation to their benifits to the human health. Callus culture of the grape, which is a natural producer of stilbenes, can serve as an effective source of these compounds. Grape’s resistance to biotic environmental stress conditions is associated with the synthesis and conversion of stilbenes, therefore the aim of the current study was to determine an interrelation between the tolerance of grape varieties to downy mildew and stilbene biosynthesis in the callus culture. Obtained results showed that there was no link between variety’s tolerance to downy mildew and content of stilbenes, chalcones and phenoloxydising enzymes in callus. Presence of the stress impact is a necessary condition for the initiation of the variety-specific synthesis of stilbenes. Callus of varieties, in which stilbenes content was higher, was characterized by a lowered expression of the chalcone synthase and chalcone isomerase relative to expression of genes of the stilbene synthase and phenylalanine ammonia liase, which confirms an interrelation between reduced competition for the substrate between two biosynthesis branches of chalcones and stilbenes with production of the latter.

Genome-wide Analysis of Root Hair Preferred RBOH Genes Suggests that Three RBOH Genes are Associated with Auxin-mediated Root Hair Development in Rice

Plant homologs of mammalian NADPH oxidase, respiratory burst oxidase homologs (RBOH), mainly oxidize NADPH to NADP+ and transfer electrons to water. The membrane residing RBOHs thus produce reactive oxygen species (ROS), which allows plants to withstand abiotic and biotic environmental stresses. To understand the spatial and temporal function of rice (Oryza sativa) RBOH genes, we performed expression analysis of nine RBOH genes using qRT-PCR and microarray data. The expression profiling data suggest that RBOH genes have diverse roles in various tissues and organs as well as responses to hormonal treatments. Among them, OsRBOH2, OsRBOH3, and OsRBOH5 are preferentially expressed in root hairs. Exogenous auxin upregulates the expression of OsRBOH1, OsRBOH2, OsRBOH3, OsRBOH4, and OsRBOH8 in root hairs, while the expression of OsRBOH7 and OsRBOH9 is downregulated. In roots, treatment with an RBOH inhibitor, diphenyleneiodonium (DPI), suppressed the accumulation of ROS in trichoblast cells which initiate root hairs, suggesting that RBOH-mediated ROS could play an important role in root hair initiation in the trichoblast cells of rice roots. Promoter analysis revealed that OsRBOH3 and OsRBOH5 contain many known cis-acting regulatory elements (CREs) associated with root hair development such as the root hair cis-acting element, RHE. OsRBOH2 and OsRBOH3, which are upregulated after treatment with indole-3-acetic acid (IAA) with significant expression in root hairs, might be key players in root hair elongation via an auxin-dependent pathway in rice.

The Nature of Programmed Cell Death

In multicellular organisms, cells are frequently programmed to die. This makes good sense: cells that fail to, or are no longer playing important roles are eliminated. From the cell’s perspective, this also makes sense, since somatic cells in multicellular organisms require the cooperation of clonal relatives. In unicellular organisms, however, programmed cell death (PCD) poses a difficult and unresolved evolutionary problem. The empirical evidence for PCD in diverse microbial taxa has spurred debates about what precisely PCD means in the case of unicellular organisms (how it should be defined). In this article, we survey the concepts of PCD in the literature and the selective pressures associated with its evolution. We show that definitions of PCD have been almost entirely mechanistic and fail to separate questions concerning what PCD fundamentally is from questions about the kinds of mechanisms that realize PCD. We conclude that an evolutionary definition is best able to distinguish PCD from closely related phenomena. Specifically, we define “true” PCD as an adaptation for death triggered by abiotic or biotic environmental stresses. True PCD is thus not only an evolutionary product but must also have been a target of selection. Apparent PCD resulting from pleiotropy, genetic drift, or trade-offs is not true PCD. We call this “ersatz PCD.”

Leaf wound induced ultraweak photon emission is suppressed under anoxic stress: Observations of Spathiphyllum under aerobic and anaerobic conditions using novel in vivo methodology.

Plants have evolved a variety of means to energetically sense and respond to abiotic and biotic environmental stress. Two typical photochemical signaling responses involve the emission of volatile organic compounds and light. The emission of certain leaf wound volatiles and light are mutually dependent upon oxygen which is subsequently required for the wound-induced lipoxygenase reactions that trigger the formation of fatty acids and hydroperoxides; ultimately leading to photon emission by chlorophyll molecules. A low noise photomultiplier with sensitivity in the visible spectrum (300–720 nm) is used to continuously measure long duration ultraweak photon emission of dark-adapting whole Spathiphyllum leaves (in vivo). Leaves were mechanically wounded after two hours of dark adaptation in aerobic and anaerobic conditions. It was found that (1) nitrogen incubation did not affect the pre-wound basal photocounts; (2) wound induced leaf biophoton emission was significantly suppressed when under anoxic stress; and (3) the aerobic wound induced emission spectra observed was > 650 nm, implicating chlorophyll as the likely emitter. Limitations of the PMT photocathode’s radiant sensitivity, however, prevented accurate analysis from 700–720 nm. Further examination of leaf wounding profile photon counts revealed that the pre-wounding basal state (aerobic and anoxic), the anoxic wounding state, and the post-wounding aerobic state statistics all approximate a Poisson distribution. It is additionally observed that aerobic wounding induces two distinct exponential decay events. These observations contribute to the body of plant wound-induced luminescence research and provide a novel methodology to measure this phenomenon in vivo.

Ubiquitin related enzymes and plant-specific ubiquitin ligase ATL family in tomato plants.

Ubiquitination is one of the fundamental post-translational modifications of proteins with ubiquitin, a conserved 76-amino acid protein present in eukaryotes, which is catalyzed by ubiquitin ligase. Compared with humans, the number of ubiquitin ligase genes is nearly double in plant species such as Arabidopsis and rice, suggesting that this enzyme plays critical roles in many aspects of plant growth, including development and abiotic and biotic environmental stress responses. In addition to its fundamental activities in eukaryotic cells, ubiquitin signaling mediates plant specific cellular functions, including phytohormone response, seed and fruit development, and biotic and abiotic stress responses. The ATL family is a RING-H2 type ubiquitin ligase widely conserved in plant species. We previously showed that the plant specific ubiquitin ligase ATL31 regulates the carbon/nitrogen-nutrient response and pathogen resistance in Arabidopsis, and we identified and characterized the basic biochemical function of an ATL31 homologue in tomato plants (Solanum lycopersicum L.). This protein, called SlATL31, may act as a ubiquitin ligase in tomato fruit. The tomato is a major crop plant and a model system for fleshy fruit development. This review provides an overview of the ubiquitin ligases and related enzymes, and highlights the ubiquitin ligase ATL family in tomato plants.

Trajectory and causes of decline in the botanical composition of dairy-grazed pasture in the Waikato

Abstract Herbage accumulation, botanical composition, tiller density and insect pest populations were monitored over 6 years for four perennial ryegrass functional types grazed by dairy cows in the Waikato. The aim was to identify genotypic and environmental factors contributing to ryegrass persistence failure in the upper North Island. Perennial ryegrass content of pastures declined as low as 60% of total herbage mass (HM) in summer of the first 3 years but recovered in autumn to at least 75%. However, following two successive severe summer/autumn droughts in Years 2 and 3, and subsequent grass grub larvae populations exceeding the damage threshold of 200/m2, by 4 years after sowing (autumn 2015), ryegrass had fallen to 20% of HM. This pattern was repeated in the following 2 years, and was not prevented by any combination of ryegrass functional type, endophyte, seeding rate, or best-practice dairy cattle grazing and soil nutrient management. The abiotic and biotic environmental stresses dominated all other factors. Keywords: pasture persistence, perennial ryegrass, drought, insects, black beetle, dairy systems

Vegetative and generative growth of groundnut genotypes under biotic environmental stress

Rahmianna AA, Yusnawan E. 2016. Vegetative and generative growth of groundnut genotypes under biotic environmental stress. Biodiversitas 17: 503-509. The decrease in groundnut pod yield is mainly influenced by disease infestation, especially bacterial wilt and foliar diseases. The objectives of this experiment were to determine the response and tolerance of groundnut genotypes to bacterial wilt, leaf spot and rust diseases, and seed infection by Aspergillus flavus. The planting materials were 25 genotypes (11 Indonesian cultivars, 12 lines introduced from ICRISAT, 1 Indonesian promising line, and 1 local cultivar) with various superiorities on diseases resistance. This study was arranged in a randomized completely block design with triplicate. The results indicated that both genotypes from ICRISAT and Indonesia had similar response to leaf spot i.e. ranging from susceptible (score 6-7) to highly susceptible (8-9). The score for rust ranged from moderately resistant to susceptible. The average pod yield was 23.1 g/plant (from 11.9 g to 29.5 g), and 13 and 12 genotypes produced pods higher and lower than the average value, respectively. ICGV 86158 and ICGV 95322 had the highest and lowest seeds as well as pod productivity, respectively. The ICRISAT genotypes were susceptible to Ralstonia solanacearum infection, except for ICGV 86590. Among the Indonesian cultivars, those with Valencia type of growth, relatively had better resistance to bacterial infection. These cultivars were also resistant to rust and A. flavus infection.

Selected Abiotic and Biotic Environmental Stress Factors Affecting Two Economically Important Sugarcane Stalk Boring Pests in the United States

Sugarcane, Saccharum spp., in the United States is attacked by a number of different arthropod pests. The most serious among those pests are two stalk boring moths in the Family Crambidae: the sugarcane borer, Diatraea saccharalis (F.), and the Mexican rice borer, Eoreuma loftini (Dyar). The two species are affected by abiotic and biotic environmental stress factors. Water deficit and excessive soil nitrogen alter physical and physiochemical aspects of the sugarcane plant that make the crop increasingly vulnerable to E. loftini. Weed growth can be competitive with sugarcane but it also supports enhanced abundances and diversity of natural enemies that can suppress infestations of D. saccharalis. In an instance where the stalk borer is considered a stress factor, proximity of vulnerable crops to sugarcane can influence levels of E. loftini infestation of sugarcane. The adverse effects of each stress factor, in terms of stalk borer attack, can be reduced by adopting appropriate cultural practices, such as adequate irrigation, judicious use of nitrogen fertilizer, using noncompetitive weed growth, and not planting vulnerable crops near sugarcane fields. Understanding the relationships between stress factors and crop pests can provide valuable insights for plant breeders and tools for incorporation into integrated pest management strategies.

The CRE1 cytokinin pathway is differentially recruited depending on Medicago truncatula root environments and negatively regulates resistance to a pathogen.

Cytokinins are phytohormones that regulate many developmental and environmental responses. The Medicago truncatula cytokinin receptor MtCRE1 (Cytokinin Response 1) is required for the nitrogen-fixing symbiosis with rhizobia. As several cytokinin signaling genes are modulated in roots depending on different biotic and abiotic conditions, we assessed potential involvement of this pathway in various root environmental responses. Phenotyping of cre1 mutant roots infected by the Gigaspora margarita arbuscular mycorrhizal (AM) symbiotic fungus, the Aphanomyces euteiches root oomycete, or subjected to an abiotic stress (salt), were carried out. Detailed histological analysis and quantification of cre1 mycorrhized roots did not reveal any detrimental phenotype, suggesting that MtCRE1 does not belong to the ancestral common symbiotic pathway shared by rhizobial and AM symbioses. cre1 mutants formed an increased number of emerged lateral roots compared to wild-type plants, a phenotype which was also observed under non-stressed conditions. In response to A. euteiches, cre1 mutants showed reduced disease symptoms and an increased plant survival rate, correlated to an enhanced formation of lateral roots, a feature previously linked to Aphanomyces resistance. Overall, we showed that the cytokinin CRE1 pathway is not only required for symbiotic nodule organogenesis but also affects both root development and resistance to abiotic and biotic environmental stresses.

Multiple abiotic stresses occurring with salinity stress in citrus

Citrus, one of the most important fruit crops in the world, is sensitive to many environmental stresses including salt stress. The negative effects of stresses often lead to poor tree growth and reductions in fruit yield and quality. Under natural conditions, citrus trees often experience multiple stresses at the same time so there are direct and indirect interactions between salinity and almost all physical abiotic stresses that include flooding, drought, nutrient deficiency, high irradiance, high temperature, and high atmospheric evaporative demand. In addition, salinity stress also has direct effects on roots predisposing trees to biotic environmental stresses including attack by root rot, nematodes and bacterial disease. The agronomical and physiological responses of citrus exposed to two or more stress factors, can differ depended on stress intensity or duration. Since citrus leaf Cl− accumulation has been linked to water use, for example, other environmental factors including high CO2 concentration, lowered temperature and high relativity humidity which decrease leaf transpiration, can improve the salt tolerance. Citrus rootstocks known to be tolerant to root rot and nematode pests, can become more susceptible to these biotic stresses when irrigated with high salinity water. Root pests can, in turn, affect the salt tolerance of citrus roots and may increase salt uptake. Moderate salinity stress, however, can reduce physiological activity and growth allowing citrus seedlings to survive cold stress and can even enhance flowering after the salinity stress is relieved. In this review, we discuss the currently available information about the effects of salinity in citrus trees from an agronomic and physiological point of view, and how these responses interact with other abiotic/physical and biotic environmental factors. Short-term potential benefits of moderate stresses including salinity, will also be discussed.

Evolutionary plasticity of insect immunity

Many insect genomes have been sequenced and the innate immune responses of several species have been studied by transcriptomics, inviting the comparative analysis of immunity-related genes. Such studies have demonstrated significant evolutionary plasticity, with the emergence of novel proteins and protein domains correlated with insects adapting to both abiotic and biotic environmental stresses. This review article focuses on effector molecules such as antimicrobial peptides (AMPs) and proteinase inhibitors, which display greater evolutionary dynamism than conserved components such as immunity-related signaling molecules. There is increasing evidence to support an extended role for insect AMPs beyond defense against pathogens, including the management of beneficial endosymbionts. The total number of AMPs varies among insects with completed genome sequences, providing intriguing examples of immunity gene expansion and loss. This plasticity is discussed in the context of recent developments in evolutionary ecology suggesting that the maintenance and deployment of immune responses reallocates resources from other fitness-related traits thus requiring fitness trade-offs. Based on our recent studies using both model and non-model insects, I propose that insect immunity genes can be lost when alternative defense strategies with a lower fitness penalty have evolved, such as the so-called social immunity in bees, the chemical sanitation of the microenvironment by some beetles, and the release of antimicrobial secondary metabolites in the hemolymph. Conversely, recent studies provide evidence for the expansion and functional diversification of insect AMPs and proteinase inhibitors to reflect coevolution with a changing pathosphere and/or adaptations to habitats or food associated with microbial contamination.

Genome-wide patterns of Arabidopsis gene expression in nature.

Organisms in the wild are subject to multiple, fluctuating environmental factors, and it is in complex natural environments that genetic regulatory networks actually function and evolve. We assessed genome-wide gene expression patterns in the wild in two natural accessions of the model plant Arabidopsis thaliana and examined the nature of transcriptional variation throughout its life cycle and gene expression correlations with natural environmental fluctuations. We grew plants in a natural field environment and measured genome-wide time-series gene expression from the plant shoot every three days, spanning the seedling to reproductive stages. We find that 15,352 genes were expressed in the A. thaliana shoot in the field, and accession and flowering status (vegetative versus flowering) were strong components of transcriptional variation in this plant. We identified between ∼110 and 190 time-varying gene expression clusters in the field, many of which were significantly overrepresented by genes regulated by abiotic and biotic environmental stresses. The two main principal components of vegetative shoot gene expression (PCveg) correlate to temperature and precipitation occurrence in the field. The largest PCveg axes included thermoregulatory genes while the second major PCveg was associated with precipitation and contained drought-responsive genes. By exposing A. thaliana to natural environments in an open field, we provide a framework for further understanding the genetic networks that are deployed in natural environments, and we connect plant molecular genetics in the laboratory to plant organismal ecology in the wild.

Genotype-by-environment interaction of barley DH lines infected with Fusarium culmorum (W.G.Sm.) Sacc.

Infection of plants by pathogens is a biotic environmental stress. Barley plants are infected, among others, by Fusarium culmorum—a pathogen affecting seedling, head, root and stem. The infection can result in reduced yield and grain quality. The aim of the study was to compare the reaction of inoculated and non-inoculated barley doubled haploids (DHs) with F. culmorum in various environments. Thirty-four genotypes were inoculated with an isolate of F. culmorum. The experiment was carried out over 6 years. Kernel weight per spike, 1000-kernel weight and percentage of plump kernels were observed in control and inoculated plants. Genotype-by-environment (GE) interaction and its structure with reference to the environments and genotypes were analysed. Additional information about the sensitivity of healthy and infected genotypes to environments was determined by the regression analysis. Statistical computation was made using the SERGEN software. Lines were considered as unstable when their GE interaction was significant at P = 0.05. Unstable genotypes were classified as intensive or extensive according to the results of the regression analysis. It was found that infection with Fusarium decreased the stability of barley lines in different environments. Interaction of unstable infected genotypes with environments, most often, could not be explained by the regression—their response to various environmental conditions appeared to be unpredictable. Selection of lines less susceptible to biotic and abiotic stresses was possible due to comparison of classification of healthy and infected lines, which was made based on their main effects and GE interaction.

Evolution of wild wheat and barley and crop improvement: Studies at the Institute of Evolution

Human civilization is based on agricultural domestication and cultivation. Evolution of crop plants from their hardy wild progenitors involved massive erosion of the original genetic resources, especially during modern agriculture, making the crops vulnerable and susceptible to abiotic and biotic environmental stresses. The wild relatives are the best hope for crop improvement because of their adaptive complexes to abiotic and biotic stresses. This is especially important in a world where the population and food demands are still increasing. Here, I briefly review the studies conducted at the Institute of Evolution, University of Haifa, during 1975-2007 on the ecological genomics and adaptive complexes of wild emmer wheat, Triticum dicoccoides, the progenitor of most wheat, and wild barley, Hordeum spontaneum, the progenitor of cultivated barley. We explored adaptive fitness components and genetic resources of wild cereal genotypes and populations in their center of origin and diversity in the Near East F...