Biotechnology Sector Research Articles

Preparation of Lyocell Fibers from Solutions of Miscanthus Cellulose.

Both annual (cotton, flax, hemp, etc.) and perennial (trees and grasses) plants can serve as a source of cellulose for fiber production. In recent years, the perennial herbaceous plant miscanthus has attracted particular interest as a popular industrial plant with enormous potential. This industrial crop, which contains up to 57% cellulose, serves as a raw material in the chemical and biotechnology sectors. This study proposes for the first time the utilization of miscanthus, namely Miscanthus Giganteus "KAMIS", to generate spinning solutions in N-methylmorpholine-N-oxide. Miscanthus cellulose's properties were identified using standard methods for determining the constituent composition, including also IR and atomic emission spectroscopy. The dry-jet wet method was used to make fibers from cellulose solutions with an appropriate viscosity/elasticity ratio. The structural characteristics of the fibers were studied using IR and scanning electron microscopy, as well as via X-ray structural analysis. The mechanical and thermal properties of the novel type of hydrated cellulose fibers demonstrated the possibility of producing high-quality fibers from miscanthus.

Microfluidic platform for microbial spore germination studies in multiple growth conditions

BackgroundSpores are highly resistant dormant cells, adapted for survival and dispersal, that can withstand unfavourable environmental conditions for extended periods of time and later reactivate. Understanding the germination process of microbial spores is important in numerous areas including agriculture, food safety and health, and other sectors of biotechnology. Microfluidics combined with high-resolution microscopy allows to study spore germination at the single-cell level, revealing behaviours that would be hidden in standard population-level studies.MethodsHere, we present a microfluidic platform – the so-called four-conditions microfluidic chemostat (4CMC) – for germination studies where spores are confined to monolayers inside microchambers, allowing the testing of four growth conditions in parallel. This platform can be used with multiple species, including non-model organisms, and is compatible with existing image analysis software.ResultsIn this study, we focused on three soil dwellers, two bacteria and one fungus, and revealed new insights into their germination. We studied endospores of the model bacterium Bacillus subtilis and demonstrated a correlation between spore density and germination in rich media. We then investigated the germination of the obligate-oxalotrophic environmental bacterium Ammoniphilus oxalaticus in a concentration gradient of potassium oxalate, showing that lower concentrations result in more spores germinating compared to higher concentrations. We also used this microfluidic platform to study the soil beneficial filamentous fungus Trichoderma rossicum, showing for the first time that the size of the spores and hyphae increase in response to increased nutrient availability, while germination times remain the same.DiscussionOur platform allows to better understand microbial behaviour at the single-cell level, under a variety of controlled conditions. While we used it to decipher the responsiveness of soil dwellers’ spores, it would also be suitable for other spores from bacteria or filamentous fungi, but also vegetative cells and yeast, and even microbial communities.

Enhancement of the Production of Tropane Alkaloids in the Hyoscyamus Niger L. Callus Using Different Biotic Elicitors

This study looked at the amount of tropane alkaloids in both the leaves of the mother plant and the callus cultures of Hyoscyamus niger L. It also looked at how biotic elicitation affects the growth of callus cultures and increases the production of alkaloids. The investigation identified and quantified three primary tropane alkaloids (Hyoscyamine, Scopolamine, and Atropine) from both sources. Remarkably, the callus cultures exhibited obviously higher levels of alkaloids in comparison to the leaves of the mother plant, implying their potential as a valuable reservoir for alkaloid production. Concerning the effects of biotic elicitation, the study showed that the application of chitosan (CHT), yeast extract (YE), and fungal extract of Trichoderma asperellum yielded a substantial decrease in callus weight when contrasted with the control group. This decrease became more pronounced with escalating concentrations of the elicitors. In terms of alkaloid synthesis, a clear correlation between the concentration of bio stimulants and tropane alkaloid levels was established. Particularly, CHT elicitation displayed the most pronounced enhancement in levels of tropane alkaloids among the three elicitors. Notably, the highest CHT concentration of 40 mg/L yielded the most elevated levels of alkaloids, measuring at 25.3 µg/g for Hyoscyamine, 31.2 µg/g for Scopolamine, and 21.5 µg/g for Atropine. This represents approximate percentage increases in concentration of 208%, 183%, and 198%, respectively, when compared to the control treatment. Generally, these findings carry significant implications for advancing tropane alkaloid biosynthesis, with potential applications spanning the pharmaceutical and biotechnology sectors.

Divergent trajectories on frontier innovations: A comparison of international venture capital-invested ventures between China and the United States

How do international venture capital (IVC)-invested ventures (IIVs) between the U.S. and China generate new knowledge differently and follow distinct growth trajectories? Given that venture capital (VC) is critical for financing frontier innovations among new ventures, comparing U.S. and Chinese IIVs is essential for informing national innovation policy. Based on the organizational ecology perspective, we propose that U.S. and Chinese new ventures have divergent trajectories in frontier innovations. We identify 1782 first-round IVC investments in U.S. and Chinese new ventures operating in the computer software, e-commerce, biotechnology, semiconductors, and artificial intelligence sectors between 2010 and 2020 along with their 2825 patents (317 Chinese patents and 2508 U.S. patents). Our findings indicate that Chinese IIVs exhibit greater variation in terms of technological distance, search scope, and knowledge coupling, compared to U.S. IIVs. However, Chinese IIVs’ frontier innovations have more divergent technological trajectories and attract fewer followers than U.S. IIVs, based on patent citations. In a firm-level analysis, we find that Chinese IIVs are less likely to receive a subsequent financing round. Further, IIVs with greater technological distance and search scope, and with higher impact patents, have more opportunities to receive the next round of funding.

Characterization and comparative genomic analysis of a marine Bacillus phage reveal a novel viral genus.

Although recent metagenomics research has obtained a wealth of phage genetic information, much of it is considered "dark matter" because of the lack of similarity with known sequences in the database. Therefore, the isolation and characterization of novel phages will help to interpret the vast unknown viral metagenome data and improve our understanding of phage diversity and phage-host interactions. Bacillus pumilus shows high economic relevance due to its wide applications in biotechnology, industry, biopharma, and environmental sectors. Since phages influence the abundance, metabolism, evolution, fitness, and ecological functions of bacteria through complex interactions, the significance of isolation and characterization of novel phages infecting B. pumilus is apparent. In this study, we isolated and characterized a B. pumilus phage belonging to a novel viral genus, which provides essential knowledge for phage biology as well as the industrial application of B. pumilus.

Expressed Protein Ligation in Flow.

The development of a flow chemistry platform for the generation of modified protein targets via expressed protein ligation (EPL) is described. The flow EPL platform enables efficient ligation reactions with high recoveries of target protein products and superior reaction rates compared to corresponding batch processes. The utility of the flow EPL technology was first demonstrated through the semisynthesis of the tick-derived chemokine-binding protein ACA-01 containing two tyrosine sulfate modifications. Full-length, sulfated ACA-01 could be efficiently assembled by ligating a recombinantly expressed C-terminal protein fragment and a synthetic sulfopeptide thioester in flow. Following folding, the semisynthetic sulfoprotein was shown to exhibit potent binding to a variety of pro-inflammatory chemokines. In a second modified protein target, we employed an in-line flow EPL-photodesulfurization strategy to generate both unmodified and phosphorylated forms of human β-synuclein by fusing a recombinant protein thioester, generated through cleavage of an intein fusion protein, and a synthetic (phospho)peptide. The semisynthetic proteins were assembled in 90 min in flow, a significant improvement over corresponding batch protein assembly, and enabled access to tens of milligrams of high purity material. Flow EPL has the potential to serve as a robust technology to streamline access to homogeneously modified proteins for a variety of applications in both academia, as well as in the pharmaceutical and biotechnology sector.

Inflammation unites diverse acute and chronic diseases.

Inflammation and immunity contribute pivotally to diverse acute and chronic diseases. Inflammatory pathways have become increasingly targets for therapy. Yet, despite substantial similarity in mechanisms and pathways, the scientific, medical, pharma and biotechnology sectors have generally focused programs finely on a single disease entity or organ system. This insularity may impede progress in innovation and the harnessing of powerful new insights into inflammation biology ripe for clinical translation. A multidisciplinary thinktank reviewed highlights how inflammation contributes to diverse diseases, disturbed homeostasis, ageing and impaired healthspan. We explored how common inflammatory and immune mechanisms that operate in key conditions in their respective domains. This consensus review highlights the high degree of commonality of inflammatory mechanisms in a diverse array of conditions that together contribute a major part of the global burden of morbidity and mortality and present an enormous challenge to public health and drain on resources. We demonstrate how that shared inflammatory mechanisms unite many seemingly disparate diseases, both acute and chronic. The examples of infection, cardiovascular conditions, pulmonary diseases, rheumatological disorders, dementia, cancer and ageing illustrate the overlapping pathogenesis. We outline opportunities to synergize, reduce duplication and consolidate efforts of the clinical, research and pharmaceutical communities. Enhanced recognition of these commonalties should promote cross-fertilization and hasten progress in this rapidly moving domain. Greater appreciation of the shared mechanisms should simplify understanding seemingly disparate diseases for clinicians and help them to recognize inflammation as a therapeutic target which the development of novel therapies is rendering actionable.

Economic Viability of Cutting-Edge Genetic Treatments: Balancing Innovation and Fiscal Sustainability in India's Healthcare Sector

Recent breakthroughs in genetic treatments offer unprecedented opportunities for addressing previously incurable conditions, but their astronomical costs present significant challenges to healthcare systems globally, particularly in developing economies like India. This comprehensive study conducts an in-depth economic analysis of three recently approved genetic treatments, meticulously comparing their long-term financial impacts with traditional care methods in the Indian healthcare context. Utilizing advanced economic modeling techniques and drawing from a diverse array of data sources including clinical studies, real-world evidence, and Indian health databases, we evaluate the treatments' cost-effectiveness, budget impact, and potential for long-term savings over an extended 30-year period. Our findings reveal a complex economic landscape: while these cutting-edge treatments require substantial initial investments, ranging from Rs. 10 crore to Rs. 18 crore per patient, they may offer significant long-term economic benefits in specific scenarios. The study goes beyond mere cost analysis, exploring the broader economic implications of these treatments, including their potential impact on workforce productivity, caregiver burden, and the development of India's biotechnology sector. We propose a comprehensive framework for assessing the economic sustainability of such high-cost treatments, taking into account both immediate budgetary constraints and potential long-term societal benefits. Furthermore, we discuss detailed policy implications for the integration of these treatments into India's healthcare system, emphasizing the need for innovative financing mechanisms, value-based pricing models, and adaptive health technology assessment methods. This research aims to provide policymakers, healthcare providers, and industry stakeholders with crucial insights to navigate the complex intersection of medical innovation and economic sustainability in the rapidly evolving landscape of genetic treatments.

Building bio-innovation systems through advanced biotechnology education.

We discuss the role of advanced biotechnology education in fostering sustainable bio-innovation systems. As a case study, we focus on Paraguay's Graduate Diploma in Innovation Management and Biotechnological Projects, which emphasizes interdisciplinary collaboration, stakeholder integration, and professionals skilled in the interplay between biotechnology, society, and governance. We highlight the relevance of educational programs in addressing the gap between academic research and industrial needs, thereby contributing to sustainable growth in the biotechnology sector.

Inter-firm alliance portfolio distance and the partners’ preferences for the distal governance modes to manage endogenous uncertainty in the biotechnology sector

Inter-firm alliances play a pivotal role in the swiftly evolving high-tech landscape, yet they often introduce uncertainty, especially when partners have varying degrees of asymmetry. This study explores the dynamics of such alliances, focusing on endogenous uncertainty (technology and partner-specific) over external factors (culture and institutions). We investigate whether the alliance portfolio distance between partners aligns with distal governance modes, which refers to the continuity from the upfront payment mode to equity investments on the timeline. Furthermore, does the investor’s comparative position influence this alignment between construed uncertainty and governance modes? Drawing on Construal Level Theory (CLT), inter-partner distance corresponds to high-level construal in distal governance modes, while proximity aligns with low-level construal in proximal modes. The analysis includes 13,000 biotechnology sector alliance events across 1,700 firms in 29 countries over a decade. Findings reveal a shift in governance mode preferences from the concrete and proximal (upfront) to abstract and distal (equity), touching milestones and licensing in the middle. The investor’s lead in the alliance portfolio asymmetry further strengthens the preference for abstract modes in the distal and high abstract construal levels. This study advances CLT as a framework, offers an integrated approach, and highlights a preference for high-value tech collaborations despite increased cost and risk over temporal and abstract governance conditions.

Keratin hydrolysates: a sustainable product in biotechnology sectors by microbial conversion

Keratin hydrolysates: a sustainable product in biotechnology sectors by microbial conversion

Cultivation modes affect the morphology, biochemical composition, and antioxidant and anti-inflammatory properties of the green microalga Neochloris oleoabundans

Microalgae are considered promising sustainable sources of natural bioactive compounds to be used in biotechnological sectors. In recent years, attention is increasingly given to the search of microalgae-derived compounds with antioxidant and anti-inflammatory properties for nutraceutical or pharmacological issues. In this context, attention is usually focused on the composition and bioactivity of algae or their extracts, while less interest is driven to their biological features, for example, those related to morphology and cultivation conditions. In addition, specific studies on the antioxidant and anti-inflammatory properties of microalgae mainly concern Chlorella or Spirulina. The present work was focused on the characterization of the Chlorophyta Neochloris oleoabundans under two combinations of cultivation modes: autotrophy and glucose-induced mixotrophy, each followed by starvation. Biomass for morphological and biochemical characterization, as well as for extract preparation, was harvested at the end of each cultivation phase. Analyses indicated a different content of the most important classes of bioactive compounds with antioxidant/anti-inflammatory properties (lipids, exo-polysaccharides, pigments, total phenolics, and proteins). In particular, the most promising condition able to prompt the production of antioxidant algal biomass with anti-inflammatory properties was the mixotrophic one. Under mixotrophy, beside an elevated algal biomass production, a strong photosynthetic metabolism with high appression of thylakoid membranes and characteristics of high photo-protection from oxidative damage was observed and linked to the overproduction of exo-polysaccharides and lipids rather than pigments. Overall, mixotrophy appears a good choice to produce natural bioactive extracts, potentially well tolerated by human metabolism and environmentally sustainable.

Polysaccharide sulfotransferases: the identification of putative sequences and respective functional characterisation.

The vast structural diversity of sulfated polysaccharides demands an equally diverse array of enzymes known as polysaccharide sulfotransferases (PSTs). PSTs are present across all kingdoms of life, including algae, fungi and archaea, and their sulfation pathways are relatively unexplored. Sulfated polysaccharides possess anti-inflammatory, anticoagulant and anti-cancer properties and have great therapeutic potential. Current identification of PSTs using Pfam has been predominantly focused on the identification of glycosaminoglycan (GAG) sulfotransferases because of their pivotal roles in cell communication, extracellular matrix formation and coagulation. As a result, our knowledge of non-GAG PSTs structure and function remains limited. The major sulfotransferase families, Sulfotransfer_1 and Sulfotransfer_2, display broad homology and should enable the capture of a wide assortment of sulfotransferases but are limited in non-GAG PST sequence annotation. In addition, sequence annotation is further restricted by the paucity of biochemical analyses of PSTs. There are now high-throughput and robust assays for sulfotransferases such as colorimetric PAPS (3'-phosphoadenosine 5'-phosphosulfate) coupled assays, Europium-based fluorescent probes for ratiometric PAP (3'-phosphoadenosine-5'-phosphate) detection, and NMR methods for activity and product analysis. These techniques provide real-time and direct measurements to enhance the functional annotation and subsequent analysis of sulfated polysaccharides across the tree of life to improve putative PST identification and characterisation of function. Improved annotation and biochemical analysis of PST sequences will enhance the utility of PSTs across biomedical and biotechnological sectors.

Purification and Characterization of a Small Thermostable Protease from Streptomyces sp. CNXK100.

Proteases derived from Streptomyces demonstrate numerous commendable properties, rendering it extensively applicable in biotechnology and various industrial sectors. This study focused on the purification and characterization of the thermostable protease obtained from Streptomyces sp. CNXK100. The purified protease exhibited an estimated molecular weight of 27 kDa, with optimal activity at 75°C and pH 8.0. Notably, the enzyme remained active even without any metal ions and fully active in the presence of Na+, K+, Mg2+, and Cu2+metal ions. The kinetic parameters were determined with a KM value of 3.13 mg/ml and a Vmax value of 3.28 × 106 U/mg. Furthermore, the protease has demonstrated notable stability when subjected to a treatment temperature of up to 65°C for 60 minutes, and across a broad pH range extending from 5.0 to 10.0. This protease also demonstrated resilience against a spectrum of harsh conditions, including exposure to organic solvents, surfactants, bleaching agents, and proteolytic enzymes. Additionally, the enzyme maintained its activity following treatment with commercial detergents, accomplishing complete thrombus lysis at a concentration of 2.50 mg/ml within 4 hours. Remarkably, the protease exhibited stability in terms of activity and protein concentration for 70 days at 4°C. These findings underscore the potential industrial applications of the thermostable protease from Streptomyces sp. CNXK100.

In – Silico Biological Evaluation of Antifungal Drugs - Molinspiration

The aim of this research work is to evaluate In – Silico Antifungal Drugs, in this work Molinspiration is used to provide predictive tools for essential molecular properties, such as lipophilicity, aqueous solubility and pKa, which are critical in drug discovery and cheminformatics. It operates based on a comprehensive database of chemical structures and employs algorithms to calculate these properties. Molinspiration is a renowned software and informatics company that plays a pivotal role in the pharmaceutical and biotechnology industries. With a primary focus on providing cutting-edge cheminformatics tools and software solutions, Molinspiration has significantly contributed to the field of drug discovery and medicinal chemistry. Molinspiration's software is an indispensable resource for the pharmaceutical and biotechnology sectors.

Nanotechnology and The Use of Nanoparticles and Its Effect on Wheat Growing

The rapid increase in the human population and the improvement in the living standards of many countries in the world cause the increasing supply of agricultural land to be unmet. Therefore, soil fertility is gaining importance day by day and soil texture is becoming a strategic element. With the development of nanotechnology, the usability of nanoparticles in many fields, especially in agriculture, has started to be investigated or discussed. Studies in agricultural nanotechnology have generally focused on using less pesticides, increasing yields or developing stress-resistant crops. Some studies in this field have started to yield positive results. However, more studies are needed for nanotechnology to be used in agriculture. Because deciding on the use of nanoparticles is an issue that can be reached in the long term. In recent years, the application of nanotechnology-based applications in agriculture is one of the strategies that attract the attention of researchers. Nanotechnology explores a wide spectrum of applications in the fields of biotechnology and agriculture sector, creating a broad spectrum for various applications. Nanotechnology has become a new technology that can be used in various industries such as industry, medicine, food science and safety, smart packaging and agriculture. Nanotechnology, which has been used in many stages of agriculture in recent years, has been widely applied in product production, development, processing, packaging, storage and transportation, and has brought about major changes in food and agricultural systems. It is also used to increase plant resistance and to control the efficacy and safety of pesticides and fertilizers. Thus, the application of nanostructured materials designed for sustainable crop production reduces nutrient losses, suppresses diseases and increases yields. In this study, the strengths, weaknesses, opportunities and targets for the use of nanotechnology and nanoparticles in wheat agriculture were identified and presented.

Effects of Cultivation Stress on the Glaucophyte Cyanophora paradoxa and Bioactive Potential in Human Cancer Cell Lines

ABSTRACT The propensity to accumulate high-value metabolites under specific conditions makes microalgae promising candidates for commercial applications. Here, the freshwater microalga Cyanophora paradoxa CCAP 981/1 was cultivated under varying conditions (control, nitrogen limitation, NaCl supplementation and blue light exposure) and the biological activity of the corresponding biomass extracts was assessed against human breast (MCF-7) and lung (A549) cancer cell lines. The stress treatments hindered the growth of C. paradoxa, the control displaying the highest specific growth rate (0.16 d−1 ± 0.01). Biomass screening by GCMS and HPLC identified diverse bioactive compounds including pigments (chlorophylls, xanthophylls, carotenes) and fatty acids (e.g., EPA). The control cultivation conditions returned the highest yields of biomass (724 mg), zeaxanthin (6.8 mg g−1), EPA (1.1 mg g−1) and phycocyanin (113.9 mg g−1), while the blue light treatment enhanced the proportions of unsaturated forms of C18 (34.3%). Extracts of dried biomass obtained by cold maceration using solvents of increasing polarity were used in the treatment of MCF-7 and A549 cancer cells. Ethyl acetate extracts from nitrogen-limited cultures reduced MCF-7 viability (~70%) after 72 h. However, the diethyl ether and methanol extracts showed no impact on MCF-7 and A549 cell viability. Furthermore, acridine orange staining revealed no significant DNA damage in these cell lines upon exposure to C. paradoxa extracts. Overall, the minimal impacts on cell viability observed here supports considering C. paradoxa as a promising source of natural bioactive compounds with applications in various biotechnological sectors.

NEW METHODS FOR STRUCTURAL DEVELOPMENT CAUSED BY OPEN INNOVATION IN RED BIOTECHNOLOGY

Red (pharmaceutical) biotechnology is currently one of the most innovative industries. A good example of this is the fight to develop a vaccine against the COVID-19 pandemic, or even the incredible dynamism of the development of anti-cancer drugs.Innovations always carry uncertainty within them - the authors of this article see and experience this every day during their managerial work related to R&D in the biotechnology sector. Decisions often have to be made on uncertain grounds, with incomplete information. Mapping all these anomalies and their root causes is also necessary according to what has been experienced in various organizations, but at the same time it is a very interesting and challenging task. One of the possible means of sharing and reducing the risk is the so-called Open innovation, which required innovations in the fields of technical, industrial rights protection, privacy protection, but also cooperation platforms.All this required a new organizational and structural operation from the actors. This means that technological innovation attracts and results in project innovations. We assume that organizational development and structural innovations were also achieved through these transfers. We are trying to validate this hypothesis with the help of interviews with professionals.Our thesis: the challenges arising from the special innovation of red biotech also caused and necessitated the innovation of organizational structures and the development of its organizational and structural functioning, to which open innovation gave outstanding help.

Construction and investigation of multi-enzyme immobilized matrix for the production of HFCS.

Enzymes are biological molecules that act as catalysts and speed up the biochemical reactions. The world's biotechnological ventures are development of enzyme productiveness, and advancement of novel techniques for thriving their shelf existence. Nowadays, the most burning questions in enzyme technology are how to improve the enzyme productivity and reuse them. The immobilization of enzymes provides an excellent scope to reuse the enzymes several times to increase productivity. The main aim of the present study is the establishment of an immobilized multi-enzyme bio-system engineering process for the production of High-fructose corn syrup (HFCS) with an industrial focus. In this study, multi-enzyme such as α-amylase, glucoamylase and glucose isomerase were immobilized in various support matrices like sodium alginate, sawdust, sugarcane bagasse, rice bran and combination of alginate with cellulosic materials. The activities of the immobilized multi-enzyme system for the production of HFCS from the starch solution were determined. The multi-enzyme immobilized in sodium alginate shows better fructose conversion than free enzyme. Among the support matrices, multi-enzyme immobilized in sawdust produced total 80.74 mg/mL of fructose from starch solution and it was able to be used in several production cycles. On the other hand, multi-enzyme immobilized in combination of sodium alginate and sawdust produced the maximum amount of fructose (total 84.82 mg/mL). The free enzyme produced 17.25 mg/mL of fructose from the starch solution in only a single cycle. In this study a new fixed bed immobilized multi-enzyme bioreactor system was developed for the production of HFCS directly from starch. This finding will create a new opportunity for the application of immobilized multi-enzyme systems in many sectors of industrial biotechnology.

Vaccine Nationalism: How China's State Media Misinform about Western Vaccines and Highlight the Successes of Chinese Vaccines to Different Audiences

What motivates state-sponsored vaccine misinformation campaigns, given clear scientific evidence of vaccines' efficacy? The authors explored this issue through the lens of state-owned presses in mainland China and in Hong Kong. They first collected an original database of media reports on both Western and Chinese vaccines from 16 Chinese-language media publications based in mainland China, Hong Kong, and Taiwan. They found the quantity of coverage of Western vaccines by mainland state-owned media outlets to be much less than their coverage of Chinese vaccines, reflecting the unavailability of Western vaccines in mainland China. However, applying a dictionary-based sentiment analysis, the authors found that state-owned presses in mainland China still portrayed Western vaccines negatively. In Hong Kong, where there is direct competition between Chinese and Western vaccines, they found that state-owned presses gave high coverage of both Western and Chinese vaccines but greater negative coverage of Western vaccines. These findings are consistent with a Chinese producer–oriented “vaccine nationalism” policy designed to nurture the domestic biotechnology sector.