Concept Of Biotechnology Research Articles

Cultivation driven transcriptomic changes in the wild-type and mutant strains of Rhodospirillum rubrum

Purple photosynthetic bacteria (PPB) are versatile microorganisms capable of producing various value-added chemicals, e.g., biopolymers and biofuels. They employ diverse metabolic pathways, allowing them to adapt to various growth conditions and even extreme environments. Thus, they are ideal organisms for the Next Generation Industrial Biotechnology concept of reducing the risk of contamination by using naturally robust extremophiles. Unfortunately, the potential of PPB for use in biotechnology is hampered by missing knowledge on regulations of their metabolism. Although Rhodospirillum rubrum represents a model purple bacterium studied for polyhydroxyalkanoate and hydrogen production, light/chemical energy conversion, and nitrogen fixation, little is known regarding the regulation of its metabolism at the transcriptomic level. Using RNA sequencing, we compared gene expression during the cultivation utilizing fructose and acetate as substrates in case of the wild-type strain R. rubrum DSM 467T and its knock-out mutant strain that is missing two polyhydroxyalkanoate synthases PhaC1 and PhaC2. During this first genome-wide expression study of R. rubrum, we were able to characterize cultivation-driven transcriptomic changes and to annotate non-coding elements as small RNAs.

Mastery concepts and misconceptions in biotechnology courses with a three tier diagnostic test

This research aims to determine the mastery of concepts and misconceptions of students in the Biology Education Study Program wich is in Pekanbaru. This type of research is survey research with a porpusive sampling type. The population of the study was all students who had taken biotechnology courses, while the research sample was 6th semester students who had just completed biotechnology courses in the previous semester. The total research sample was 32 students. The instrument used to measure mastery of concepts and misconceptions is a diagnostic test with 20 multiple choice questions accompanied by reasons and level of belief. The questions are given using a Google form and students work on the questions during class and are given 90 minutes.The research data were analyzed descriptively. Based on the results showed that for indicator 1 30.01% of students understood the basic concepts of biotechnology well and 55.63 had misconceptions. In indicator 2, only 5% of students understood the concept of biotechnology process while 64.38% had misconceptions and 29.38 did not understand the concept. Indicator 3, as many as 10.63% of students understand the concept of conventional and modern biotechnology characteristics and 59.38% of students have misconceptions and 25.63% do not understand the concept while indicator 4 as many as 30.63% of students understand the examples of biotechnology products while still 43.13% have misconceptions and 22.50% still do not understand the concept. Students' mastery of concepts in biotechnology courses is in a very low category and while more than half of the students still experience misconceptions and do not understand the concepts.

Spatial distribution of bioeconomy R&D funding: opportunities for rural and lagging regions?

ABSTRACT The promotion of R&D carries the risk that economically strong regions will benefit to a greater extent and that regional disparities will increase by focusing the economy on high technology. However, this is expected to depend on the specific sectoral and technological composition of R&D funding. R&D funding for a bio-based economy in Germany is particularly illustrative for a shift in funding focus from a narrow concept of biotechnology to a broader concept of bioeconomy. Along with this shift, an impact on the spatial distribution of R&D funding is assumed. Against the background of inclusive innovation policy, this study examines the potential of the bioeconomy for a reduction of regional disparities in public R&D funding. Based on a database containing publicly-funded R&D projects in Germany and further regional data, comparative regressions are conducted in order to identify spatial patterns. The results demonstrate distinct funding mechanisms in the different areas of the bioeconomy. The broadening of R&D funding for bio-based activities from the biotechnology vision towards bioresources and bioecology leads to a greater participation of rural and lagging regions which is expected to be the result of the inclusion of more traditional industries as recipients of R&D funding.

Teaching and Learning Biotechnology at University of Rwanda - College of Science and Technology: The Assessment of Teaching Practices and Learning Styles for Biotechnology Concepts Understanding

Teaching and Learning Biotechnology at University of Rwanda - College of Science and Technology: The Assessment of Teaching Practices and Learning Styles for Biotechnology Concepts Understanding

From Farm to Classroom: Tubers as Key Resources in Developing Biology Learning Media Rooted in Banten's Local Culture

This study aims to investigate the diversity of tuber plants used as food by the people in Banten, which are then used as learning content based on local potential. To achieve this goal, we conducted a research and development (RD) method to create various media using the information of tubers diversity in Banten. In the first stage, we collected data on the diversity of tuber plants cultivated by residents in Banten province and their uses. In the second stage, we developed various forms of learning media from the diversity data and the status of plant utilization. Data on diversity was obtained by conducting field observation and interviews with 373 respondents: tuber farmers, traders, intermediaries, processed makers, souvenir shop owners, and homemakers in Banten. Data were analyzed using the descriptive-qualitative method. Information about the diversity of types of tubers, their use for food, and their processing techniques then use as the content on learning media of Biology subject for senior high school on biodiversity, ecosystems, and biotechnology concept. We customize content development to support Biology classes using the Kurikulum Merdeka. The results showed that there were 16 species of tubers used as food, belong to 11 families, such as Dioscoreaceae, Euphorbiaceae, and Convolvulaceae which used as a staple food, snacks, and vegetables. The results of the media development analysis show that diversity data can be applied to various types of multimedia learning media in the biological sciences. While these materials may complement the learning of Biology, careful consideration must be given to the advantages and disadvantages of each medium to ensure that they are appropriate to the learning goals and circumstances of the learners.

Upaya Meningkatkan Kemampuan Guru IPA dalam Mendesain Pembelajaran Berbasis Kearifan dan Potensi Lokal

Purpose: Carry out training to improve the ability of science teachers to design teaching materials based on local wisdom and local potential. Methodology/approach: Community Service activities are carried out 3 times (2 online meetings and 1 offline meeting) with a lesson study pattern (planning, doing, seeing). There are around 50 science teachers in online activities from all over Indonesia, while offline activities are carried out for science teachers in Cirebon and surrounding areas. Result: The results of community service activities are 1). Produced several teaching materials based on local wisdom and local potential from various regions where participants come from, 2). Positive responses from science teachers about learning based on local wisdom and local potential, 3). From the open lesson activity, the Observer teacher stated that the learning was carried out very well (100%). 4). From reflection activities, teachers see that students learn enthusiastically and teachers learn about making and implementing learning based on local wisdom and potential. Limitation: The local potential-based learning chosen by the teacher is the biotechnology concept of Cirebon Bakung tape and Kuningan tape. The instruments used are teacher questionnaires and observation sheets. Contribution: Community service activities can provide insight for teachers to create and implement learning based on local wisdom and local potential

The research finding of marine fungi as milkfish feed and its utilization for biotechnology digital magazine

This study aimed to determine the utilization of research results of the selection of marine fungi for milkfish (Chanos chanos) feed. The study was conducted in stages, starting with identifying the issues in the learning activities of biotechnology concepts, followed by experiments to identify research methods and products, selecting an adaptation of research findings as the learning source content, and application and development of research findings as learning. The data and information were gathered through an interview grid and a questionnaire, and the findings of the surveys were then analyzed qualitatively. The results of a selection study of marine fungi from Dua Island, Banten, Indonesia as feed for milkfish related to basic competency (KD) 3.10 and 4.10 can be used as learning resources as digital magazines for class XII high school students. Thus, media can help student in learning process.

Need Analysis for Developing a Natural Science Learning Website with the Theme of Biotechnology in Improving Digital Literacy

The development of technological rapidly changes the learning process more broadly. Demands for students or prospective science teachers to improve their digital literacy skills with the help of website-based media are needed in the learning process so that it can take place optimally. The development of biotechnology has also developed rapidly in the last few years, thus demanding that students or prospective science teachers be more responsive to information and be able to present this information interactively. Unfortunately, many students or prospective science teachers are not able to understand biotechnology because it is considered that the concept of biotechnology is difficult to understand. For this reason, it is necessary to have a needs analysis to find out the aspects needed and develop the learning and learning resources needed to improve digital literacy skills and understand the concept of biotechnology. The aim of this study is to analyze the needs of learning media based on a biotechnology theme website that would be developed, so that it can improve the digital literacy skills of students or prospective science teachers. The needs analysis was carried out as a preliminary study on Borg & Gall's research and development methods, which are included in the research and information collecting stage. The analysis of the needs for developing learning tools includes student analysis, concept analysis, task analysis, and learning objectives analysis. This needs analysis is accompanied by a literature review and observation results. The results obtained are a thorough and real needs analysis on the development of a biotechnology theme website to improve the digital literacy abilities of science teacher candidates, which can be applied at the next research and development stage

Biotechnology content in pharmacy curricula: Focus on Arab Countries

IntroductionPharmaceutical biotechnology involves using the principles of biotechnology to develop drugs. With the rapid increase in biopharmaceutical products being developed and approved for use, educating pharmacy students about biotechnological products becomes paramount. However, there is a scarcity in the literature exploring biotechnology content in pharmacy education. This paper aims to explore and discuss previously published studies on biotechnology education in pharmacy curricula and will provide a brief overview of biotechnology content offered in pharmacy schools in Arab countries. PerspectiveThe majority of pharmacy schools in the United States and Europe offer biotechnology-related content within the curriculum as part of other courses. It has also been reported that biotechnology content is taught in 22 pharmacy schools in Arab countries. In general, biotechnology content is mostly taught as part of other pharmacy courses and is often provided as compulsory content. Including pharmaceutical biotechnology concepts in pharmacy curriculum has positive impact on pharmacy students as it increases their knowledge of biotechnology and their interest in the topic. ImplicationPharmaceutical biotechnology is an important field in which pharmacy graduates should be knowledgeable. However, there is a need to explore biotechnology content offered in pharmacy schools in other parts of the world in order to have an understanding of how different pharmacy programs prepare their students for practice. The information currently available in the literature is not enough to determine the usefulness of pharmaceutical biotechnology content currently offered to prepare pharmacists for practice. This highlights the need for further research in the area.

Research Implementation of the Milkfish Feed Development from the Edible Mushrooms for a Digital-Based Module in Biotechnology Concept for High School Students

Research Implementation of the Milkfish Feed Development from the Edible Mushrooms for a Digital-Based Module in Biotechnology Concept for High School Students

Implementation of the Project Based Learning (PJBL) to Increase Students Learning Interest and Learning Outcomes in Biotechnology Concept

Implementation of the Project Based Learning (PJBL) to Increase Students Learning Interest and Learning Outcomes in Biotechnology Concept

Sikap Siswa terhadap STEM : Hubungannya dengan Hasil Belajar Kognitif dalam Pembelajaran IPA

This study aims to describe students' attitudes towards STEM, cognitive learning outcomes and the relationship between the two variables. This research was quantitative, type of pre-experiment one group pre-test post-test design. The number of samples in this study was 55 class IX students taken from a total population of 192 students. The research instrument consisted of a questionnaire to measure students' attitudes towards STEM and multiple-choice tests to assess students' cognitive learning outcomes. Data were analyzed using N-Gain and person product moment correlation. The results showed that STEM-based learning improved students' attitudes toward STEM and cognitive learning outcomes in science learning. The average score obtained in the pre-test and post-test showed that students' attitudes towards STEM were most dominant in the technology dimension and the lowest in the mathematics dimension. Meanwhile, cognitive learning outcomes in science learning showed that students could master the concept of biotechnology with the highest score increase in the indicator explaining the basic principles of biotechnology. In addition, this approach proves a significant relationship between students' attitudes towards STEM and cognitive learning outcomes in science learning with an R-value of 0,514.

Ecotechnology of Hilsa-Seed Productions Digitally Under Fresh-Water-Ecology Whereas Hilsa-Seeds Can Be Boon To Experiment In Marine-Cages For Growths World-Wide, Which-Ever Countries May Opt The Best

Since decades authors are bit panicked from the then theory of Central dogma unless the rescue actions taken by all the immense bio-molecules of fats and lipids of Hilsa species that savoir mankind. Hilsa as a diet can adequately support and can protect the esteem mankind from any disasters and dealt the theory ‘Beyond the central dogma’ by virtue of developing all necessary immunities for every peaceful human being of mankind. All we may know protein may be immense needful however most proteins are hazardous unless the same is well protected with Fats. Hilsa species is such a species can provide adequate quality Fats to mankind and help us to remain protected from any evil of proteins or Virus. May be our esteem need to popularize Hilsa species globally that goes to marine. Hilsa often a marine species grown most time in marine environments under all known water qualities. Natural migration of Hilsa owing to seed production may not possible in all marine environments since migration are extremely slow in marine environments and takes prolonged times before the species to the other part of the world since aging Hisla survives around 10 – 12 years prior to that species re-enter the well known fresh waters of certain qualities unlike marine routes. Hence Hilsa species remained confined in few marine pockets only and can be inducted elsewhere marine cages, Hilsa breed under fresh waters conditions when Hilsa seeds produced under controlled Ecotechnology and again can be recruited in marine cages worldwide. All fish migrates owing to physiological requirements. This migration is based on water qualities, food and light and temperature. Usually Juvenille Hilsa species prefer a high TDS marine environments. During the breeding seasons Hilsa migrates to the as low as low TDS (< 110ppm) of waters and whenever species get a suitable osmotic-pressure or optimum Total Dissolved Solids i.e. TDS then Hilsa to breed. According to all water qualities with ranges of different parameters, intensity of light and coolness temperature are the criteria in Ecotechnology of Hilsa Breeding. In earlier days attempts were made reversedly when adult Hilsa species were tried to grow in fresh-waters and all Hilsa could not survived and projects remained unsuccessful. Present communications with mere IT, Ecotechnology, and biotechnological concepts can popularize Hilsa. May Hilsa in marine cages of TDS in a range of 190 TO 550 ppm get successful since its’ immense scientific ease globally. Hilsa may be the best quality fish so far known today due its high quality bio-molecular substances and prolific values.

The advance of industrial biotechnology in the State of Amazonas

Industrial Biotechnology has been seen as the production of the future, and can be an object of study and organizational strategy. The objective of this research is to analyze the development of industrial biotechnology in the State of Amazonas. This is a research with a quali-quanti approach with an exploratory and descriptive approach. Mapping of existing companies and research institutes in the State of Amazonas was carried out, through bibliographic and exploratory research on websites and official sites. The results showed that in the State of Amazonas, only 4 companies appear on the Biotech Map (2023), of which three are national and one is a startup. But, during the research, it was verified the existence of several other companies in the state of Amazonas that have productive activities focused on the concepts of industrial biotechnology, of which some were listed and presented in this study.

Implementasi Sumber Belajar Bioteknologi Berbasis Etnosains di Madrasah Ibtidaiyah Al-Munawaroh

Research objectives to determine the application of ethnoscience based biotechnology learning resources in learning and describes the implementation of the ethnoscience-based biotechnology learning process. The research method uses a qualitative descriptive research design. Subjects were 49 students of class VI Madrasah Ibtidaiyah Al-Munawaroh research object of ethnoscience-based biotechnology learning resources. data collection techniques with interviews and observation. Data analysis techniques with data collection, reduction, presentation, and conclusions. The results of data analysis showed that 49.8% of students did not understand the concept of biotechnology, the manufacturing process, the microorganisms involved, the mechanism of the fermentation process in tempe, peuyeum and bread. 100% of students know tempe, peuyeum, bread and tempe is consumed every day and the way of making it was handed down by our ancestors from generation to generation. 73% of students have not been able to provide examples of other biotechnology products and the microorganisms that play a role. Conclusion Madrasah Ibtidaiyah Al-Munawaroh has implemented ethnoscience-based biotechnology learning resources in learning but the implementation of students' ethno-science-based biotechnology learning processes is unable to explain the link between local wisdom culture and natural science.

A promoter toolbox for tissue-specific expression supporting translational research in cassava (Manihot esculenta).

There is an urgent need to stimulate agricultural output in many tropical and subtropical countries of the world to combat hunger and malnutrition. The starchy crop cassava (Manihot esculenta), growing even under sub-optimal conditions, is a key staple food in these regions, providing millions of people with food. Cassava biotechnology is an important technique benefiting agricultural progress, but successful implementation of many biotechnological concepts depends on the availability of the right spatiotemporal expression tools. Yet, well-characterized cassava promoters are scarce in the public domain. In this study, we investigate the promoter activity and tissue specificity of 24 different promoter elements in stably transformed cassava plants. We show that many of the investigated promoters, especially from other species, have surprisingly low activity and/or tissue specificity, but feature several promoter sequences that can drive tissue-specific expression in either autotrophic-, transport- or storage tissues. We especially highlight pAtCAB1, pMePsbR, and pSlRBCS2 as strong and specific source promoters, pAtSUC2, pMeSWEET1-like, and pMeSUS1 as valuable tools for phloem and phloem parenchyma expression, and pStB33, pMeGPT, pStGBSS1, as well as pStPatatin Class I, as strong and specific promoters for heterotrophic storage tissues. We hope that the provided information and sequences prove valuable to the cassava community by contributing to the successful implementation of biotechnological concepts aimed at the improvement of cassava nutritional value and productivity.

Fundaments and Concepts on Screening of Microorganisms for Biotechnological Applications. Mini Review

Microbial biotechnology uses microorganisms and their derivatives to generate industrial and/or environmental products that impact daily life. Modern biotechnology uses proteomics, metabolomics, quantum processors, and massive sequencing methods to yield promising results with microorganisms. However, the fundamental concepts of microbial biotechnology focus on the specific search for microorganisms from natural sources and their correct analysis to implement large-scale processes. This mini-review focuses on the methods used for the isolation and selection of microorganisms with biotechnological potential to empathize the importance of these concepts in microbial biotechnology. In this work, a review of the state of the art in recent years on the selection and characterization of microorganisms with a basic approach to understanding the importance of fundamental concepts in the field of biotechnology was carried out. The proper selection of isolation sources and the design of suitable selection criteria according to the desired activity have generated substantial changes in the development of biotechnology for more than three decades. Some examples include Taq polymerase in the PCR method and CRISPR technology. The objective of this mini review is to establish general ideas for the screening of microorganisms based on basic concepts of biotechnology that are left aside in several articles and maintain the importance of the basic concepts that this implies in the development of modern biotechnology.

Trends of the last 20 years of Postgraduate Theses in the Field of Science Education related to Biotechnological concepts in Turkey: A Content Analysis

This study made for concepts related to biotechnology research trends between the years 2000-2020 graduate thesis in the field of science in Turkey, offers a descriptive content analysis. Within the aim of the study, a total of 65 postgraduate theses were examined by scanning the database of the YOK National Thesis Center. In this study, in which document analysis, one of the qualitative research designs, was used, theses were analyzed according to years, universities, study subjects, concepts, methods, data collection tools, sampling and sample size. According to the results obtained from the findings, it has been observed that the theses are predominantly master's theses, the postgraduate theses made in the field of science education related to biotechnological concepts were carried out in a total of 27 universities and the theses were mainly done in Gazi University, İnönü University and Bolu Abant Izzet Baysal University. According to the subject distribution, it has been observed that the theses are predominantly focused on learning, attitude/perception/interest and teaching. In addition, it has been concluded that quantitative studies are mostly used in the examined theses, and empirical designs among quantitative research methods are preferred more than non-experimental designs. As data collection tools, mostly skill, attitude, perception, personality, etc. tests, achievement tests, Likert type questionnaires and semi-structured interviews were used and it was observed that the most preferred sample group was university and high school students and the sample size was mostly between 101-300, 31-100. Based on the results of the study, researchers can focus on more detailed analysis in biotechnology education through meta-analysis.

Think outside the box: 3D bioprinting concepts for biotechnological applications – recent developments and future perspectives

3D bioprinting – the fabrication of geometrically complex 3D structures from biocompatible materials containing living cells using additive manufacturing technologies – is a rapidly developing research field with a broad range of potential applications in fundamental research, regenerative medicine and industry. Currently, research into 3D bioprinting is mostly focused on new therapeutic concepts for the treatment of injured or degenerative tissue by fabrication of functional tissue equivalents or disease models, utilizing mammalian cells. However, 3D bioprinting also has an enormous potential in biotechnology. Due to the defined spatial arrangement of biologically active (non-mammalian) cells in a biomaterial matrix, reaction compartments can be designed according to specific needs, or co-cultures of different cell types can be realized in a highly organized manner to exploit cell-cell interactions. Thus, 3D bioprinting technology can enable new biotechnological concepts, for example, by implementing perfusion systems while protecting shear sensitive cells or performing cascaded bioreactions. Here, we review the use of 3D bioprinting to manufacture defined 3D microenvironments for biotechnological applications using bacteria, fungi, microalgae, plant cells and co-cultures of different cell types. We discuss recent approaches to apply 3D bioprinting in biotechnological applications and – as it is a particular challenge – concepts for the real-time monitoring of the physiological state, growth and metabolic activity of the embedded cells in 3D bioprinted constructs. With these insights, we outline new applications of 3D bioprinting in biotechnology, engineered living materials and space research.

Development of a Freeze-Dried CRISPR-Cas12 Sensor for Detecting Wolbachia in the Secondary Science Classroom.

Training the future synthetic biology workforce requires the opportunity for students to be exposed to biotechnology concepts and activities in secondary education. Detecting Wolbachia bacteria in arthropods using polymerase chain reaction (PCR) has become a common way for secondary students to investigate and apply recombinant DNA technology in the science classroom. Despite this important activity, cutting-edge biotechnologies such as clustered regularly interspaced short palindromic repeat (CRISPR)-based diagnostics have yet to be widely implemented in the classroom. To address this gap, we present a freeze-dried CRISPR-Cas12 sensing reaction to complement traditional recombinant DNA technology education and teach synthetic biology concepts. The reactions accurately detect Wolbachia from arthropod-derived PCR samples in under 2 h and can be stored at room temperature for over a month without appreciable degradation. The reactions are easy-to-use and cost less than $40 to implement for a classroom of 22 students including the cost of reusable equipment. We see these freeze-dried CRISPR-Cas12 reactions as an accessible way to incorporate synthetic biology education into the existing biology curriculum, which will expand biology educational opportunities in science, technology, engineering, and mathematics.