Bioremediation Products Research Articles

Potential routes of plastics biotransformation involving novel plastizymes revealed by global multi-omic analysis of plastic associated microbes

Despite increasing efforts across various disciplines, the fate, transport, and impact of synthetic plastics on the environment and public health remain poorly understood. To better elucidate the microbial ecology of plastic waste and its potential for biotransformation, we conducted a large-scale analysis of all publicly available meta-omic studies investigating plastics (n = 27) in the environment. Notably, we observed low prevalence of known plastic degraders throughout most environments, except for substantial enrichment in riverine systems. This indicates rivers may be a highly promising environment for discovery of novel plastic bioremediation products. Ocean samples associated with degrading plastics showed clear differentiation from non-degrading polymers, showing enrichment of novel putative biodegrading taxa in the degraded samples. Regarding plastisphere pathogenicity, we observed significant enrichment of antimicrobial resistance genes on plastics but not of virulence factors. Additionally, we report a co-occurrence network analysis of 10 + million proteins associated with the plastisphere. This analysis revealed a localized sub-region enriched with known and putative plastizymes—these may be useful for deeper investigation of nature’s ability to biodegrade man-made plastics. Finally, the combined data from our meta-analysis was used to construct a publicly available database, the Plastics Meta-omic Database (PMDB)—accessible at plasticmdb.org. These data should aid in the integrated exploration of the microbial plastisphere and facilitate research efforts investigating the fate and bioremediation potential of environmental plastic waste.

Bioremediation of Petroleum Hydrocarbons in Seawater: Prospects of Using Lyophilized Native Hydrocarbon-Degrading Bacteria.

This work aimed to develop a bioremediation product of lyophilized native bacteria to respond to marine oil spills. Three oil-degrading bacterial strains (two strains of Rhodococcus erythropolis and one Pseudomonas sp.), isolated from the NW Portuguese coast, were selected for lyophilization after biomass growth optimization (tested with alternative carbon sources). Results indicated that the bacterial strains remained viable after the lyophilization process, without losing their biodegradation potential. The biomass/petroleum ratio was optimized, and the bioremediation efficiency of the lyophilized bacterial consortium was tested in microcosms with natural seawater and petroleum. An acceleration of the natural oil degradation process was observed, with an increased abundance of oil-degraders after 24 h, an emulsion of the oil/water layer after 7 days, and an increased removal of total petroleum hydrocarbons (47%) after 15 days. This study provides an insight into the formulation and optimization of lyophilized bacterial agents for application in autochthonous oil bioremediation.

Biodegradation of crude oil by immobilized Exiguobacterium sp. AO-11 and shelf life evaluation

Exiguobacterium sp. AO-11 was immobilized on bio-cord at 109 CFU g−1 carrier for the removal of crude oil from marine environments. To prepare a ready-to-use bioremediation product, the shelf life of the immobilized cells was calculated. Approximately 90% of 0.25% (v/v) crude oil removal was achieved within 9 days when the starved state of immobilized cells was used. The oil removal activity of the immobilized cells was maintained in the presence of oil dispersant (89%) and at pH values of 7–9. Meanwhile, pH, oil concentration and salinity affected the oil removal efficacy. The immobilized cells could be reused for at least 5 cycles. The Arrhenius equation describing the relationship between the rate of reaction and temperature was validated as a useful model of the kinetics of retention of activity by an immobilized biocatalyst. It was estimated that the immobilized cells could be stored in a non-vacuum bag containing phosphate buffer (pH 7.0) at 30 °C for 39 days to retain the cells at 107 CFU g−1 carrier and more than 50% degradation activity. These results indicated the potential of using bio-cord-immobilized crude oil-degrading Exiguobacterium sp. AO-11 as a bioremediation product in a marine environment.

Bioballs carrying a syntrophic Rhodococcus and Mycolicibacterium consortium for simultaneous sorption and biodegradation of fuel oil in contaminated freshwater

Nonpathogenic effective bacterial hydrocarbon degraders, Rhodococcus ruber S103, Mycolicibacterium parafortuitum J101 and Mycolicibacterium austroafricanum Y502, were isolated from mixed polycyclic aromatic hydrocarbon (PAH)-enriched river sediments. They possessed broad substrate specificities toward various PAHs and aliphatic compounds as sole carbon sources. These strains exhibited promising characteristics, including biosurfactant production, high cell hydrophobicity, biofilm formation and no antagonistic interactions, and contained genes encoding hydrocarbon-degrading enzymes. The mixed bacterial consortium combining S103, J101 and Y502, showed more effective syntrophic degradation of two types of refined petroleum products, diesel and fuel oils, than monocultures. The defined consortium immobilized on plastic balls achieved over 50% removal efficiency of high fuel oil concentration (3000 mg L−1) in a synthetic medium and contaminated freshwater. Furthermore, the immobilized cells simultaneously degraded more than 46% of total fuel oil adsorbed on plastic balls in both culture systems. SEM imaging confirmed that the immobilized consortium exhibited biofilm formation with the bacterial community covering most of the bioball surface, resulting in high bacterial survival against toxic contaminants. The results of this study showed the potential use of the cooperative interaction between Rhodococcus and Mycolicibacterium as immobilized bioballs for the bioremediation of fuel oil-contaminated environments. Additionally, this research has motivated further investigations into the development of bioremediation products for fuel oil degradation.

In Vitro Bioaccessibility Assessment as a Tool to Predict the Toxicity of Bioremediation Products

The demand for the development of bioremediation processes designed to maintain healthy environments has increased; however, evaluation of the toxicity of its products is needed. Therefore, the toxicity of the Cd-loaded beads of the bioremediation approach developed in this paper was evaluated by using in vitro human gastrointestinal simulation (BARGE method). Cd-loaded beads were obtained from adsorption experiments of Cd from artificial groundwater (AGW) and natural river water (NRW, Walkham River, England) using Ca-alginate beads containing live cells of the mutant Brevibacillus agri C15 CdR and its wild type B. agri C15, in batch flasks. The results showed that the Ca-alginate beads containing the mutant adsorped a significant concentration of Cd (1700 mmolal), related to its adsorption capacity. Cd-loaded beads had higher concentrations of Ca and Na (2030 ± 40 and 4300 ± 18 molal, respectively), related to its composition. The effects of the gastrointestinal simulation showed that Ca had the highest bioaccessible concentrations from Cd-loaded beads of all tested elements (Al, Ca Co, Cu, Fe, K Mg, Na, and Zn) from (1280 ± 13.00 molal); while some other elements were not detected at the end of the gastrointestinal system. Cd bioaccessibility was significantly lower in the Cd-loaded beads containing the mutant (0.17 and 0.14 molal in the gastric and gastrointestinal phases, respectively), compared to the wild type (0.23 and 0.19 molal, respectively). The bioaccessible fractions (BAFs) of Cd were significantly lower in the Cd-loaded beads containing the mutant at the gastric and gastrointestinal phase, with the mean of 4.85 % and 2.95 %, respectively. The low percentages of BAFs of Cd suggested that the products of the bioremediation process developed in this project might not be relevant as a human health risk.

Bioremediation of hydrocarbon-contaminated soil from Carlini Station, Antarctica: effectiveness of different nutrient sources as biostimulation agents

Logistics and scientific activities carried out in Antarctic stations entail the risk of contamination by fuels. Among remediation strategies, biostimulation significantly improves the efficiency of hydrocarbons (HCs) removal. A 1-year-long field trial was performed in mesocosms filled with soil chronically contaminated with HCs. Three nutrient sources were evaluated as biostimulation agents: inorganic salts (with and without aeration by mixing), a slow-release granular fertilizer (Nitrofoska®) and a commercial bioremediation product (OSEII®). Their performance was assessed considering the number of culturable bacteria, the changes induced in the structure of bacterial communities, the HCs removal efficiencies and the estimation of the abiotic and biodegradative losses of HCs. The soil indigenous microbiota reduced the concentration of hydrocarbons by up to 50% in 50 days and 87% in 365 days depending on the biostimulation agent used. OSEII® (a mixture of surfactants, nutrients, and enzymes) performed better in the medium term, promoting bacterial growth and rapidly inducing changes in the structure of bacterial community, and Nitrofoska® proved to be more efficient for long-term processes, less affecting the size and structure of the microbiota. A mixed strategy combining the fastest action of commercial products acting during summer with slow-release fertilizers acting throughout the year is proposed as a long-term bioremediation treatment for Antarctic areas where the temperature rises above the freezing point and the ground is free of snow shortly during summer. This study highlights the importance of conducting research to develop remediation processes compatible with the Antarctic Treaty, exploiting the metabolic potential of the indigenous microbiota.

In Vitro Bioaccessibility Assessment as a Tool to Predict the Toxicity of Bioremediation Products

In Vitro Bioaccessibility Assessment as a Tool to Predict the Toxicity of Bioremediation Products

Optimization of an Autochthonous Bacterial Consortium Obtained from Beach Sediments for Bioremediation of Petroleum Hydrocarbons

Oil spill pollution remains a serious concern in marine environments and the development of effective oil bioremediation techniques are vital. This work is aimed at developing an autochthonous hydrocarbon-degrading consortium with bacterial strains with high potential for hydrocarbons degradation, optimizing first the growth conditions for the consortium, and then testing its hydrocarbon-degrading performance in microcosm bioremediation experiments. Bacterial strains, previously isolated from a sediment and cryopreserved in a georeferenced microbial bank, belonged to the genera Pseudomonas, Rhodococcus and Acinetobacter. Microcosms were assembled with natural seawater and petroleum, for testing: natural attenuation (NA); biostimulation (BS) (nutrients addition); bioaugmentation with inoculum pre-grown in petroleum (BA/P) and bioaugmentation with inoculum pre-grown in acetate (BA/A). After 15 days, a clear blending of petroleum with seawater was observed in BS, BA/P and BA/A but not in NA. Acetate was the best substrate for consortium growth. BA/A showed the highest hydrocarbons degradation (66%). All bacterial strains added as inoculum were recovered at the end of the experiment. This study provides an insight into the capacity of autochthonous communities to degrade hydrocarbons and on the use of alternative carbon sources for bacterial biomass growth for the development of bioremediation products to respond to oil spills.

Optimization of bioremediation-cocktail for application in the eco-recovery of crude oil polluted soil

Background: Environmental sustainability is the driver for finding the optimal bioremediation cocktail with the combination of highly potent hydrocarbonoclastic strains and the nutrient additives that significantly enhance mineralization of crude oil in polluted soil in order to mitigate its deleterious effects on the environment. In this study, four hydrocarbon-degrading bacterial strains were pre-selected from mined rhizobacterial isolates in aged crude oil-contaminated soil. Method: Agrowaste residues of poultry-droppings, corn chaff, and plantain peel were selected among others for their ability to support high biomass of selected bacterial strains. Baseline proximate analysis was performed on the agrowaste residues. Simplified, one variable at a time (OVAT) was employed in the validation of the variables for optimization using the Multivariate analysis tool of Response Surface Methodology (RSM). To test the significant formulation variables, the Box-Behnken approach using 15 runs design was adopted. Results: The rate of contaminant removal was observed to fit into a quadratic function. For optimal rate or contaminant removal, the fitted model predicted the optimal formulation cocktail condition to be within 0.54 mg/kg (Corn steep liquor), phosphate 137.49 mg/kg (poultry droppings) and 6.4% inocula for initial TPH of 9744 mg kg -1 and THC of 9641 mg kg -1 contaminant level. The model for the application of the bioremediation product and the variables evaluated had a significant p-value < 0.005 for the attainment of 85 to 96 % of TPH and THC removal after 56 days of treatment. Conclusions: This study has shown the need to harness the abundant agrowaste nutrients in supporting high throughput rhizobacteria in the formulation of a bioremediation agent suitable for use in the reclamation of oil spill sites in the Niger Delta oil-producing region.

Optimization of bioremediation-cocktail for application in the eco-recovery of crude oil polluted soil.

Background: Environmental sustainability is the driver for finding the optimal bioremediation cocktail with the combination of highly potent hydrocarbonoclastic strains and the nutrient additives that significantly enhance mineralization of crude oil in polluted soil in order to mitigate its deleterious effects on the environment. In this study, four hydrocarbon-degrading bacterial strains were pre-selected from mined rhizobacterial isolates in aged crude oil-contaminated soil. Method: Agrowaste residues of poultry-droppings, corn chaff, and plantain peel were selected among others for their ability to support high biomass of selected bacterial strains. Baseline proximate analysis was performed on the agrowaste residues. Simplified, one variable at a time (OVAT) was employed in the validation of the variables for optimization using the Multivariate analysis tool of Response Surface Methodology (RSM). To test the significant formulation variables, the Box-Behnken approach using 15 runs design was adopted. Results: The rate of contaminant removal was observed to fit into a quadratic function. For optimal rate or contaminant removal, the fitted model predicted the optimal formulation cocktail condition to be within 0.54 mg/kg (Corn steep liquor), phosphate 137.49 mg/kg (poultry droppings) and 6.4% inocula for initial TPH of 9744 mg kg -1 and THC of 9641 mg kg -1 contaminant level. The model for the application of the bioremediation product and the variables evaluated had a significant p-value < 0.005 for the attainment of 85 to 96 % of TPH and THC removal after 56 days of treatment. Conclusions: This study has shown the need to harness the abundant agrowaste nutrients in supporting high throughput rhizobacteria in the formulation of a bioremediation agent suitable for use in the reclamation of oil spill sites in the Niger Delta oil-producing region.

Application of chemical reagents as innovative remediation technologies for groundwater impacted by petroleum hydrocarbons in Italy

Innovative technologies with injection of chemical compounds were applied in order to remediate 28 contaminated sites with petroleum hydrocarbon impacted groundwater spread in the Italian territory. The used reagents can be classified into three main technologies: enhanced bioremediation products with oxygen released, In Situ Chemical Oxidation (ISCO) and surfactants. The analysis of these technologies allowed to better understand how to conduct the design, the execution and monitoring phases of the remediation. For the design phase soil and groundwater samples were collected from each site in order to identify the magnitude of the contamination and different calculation methods were tested to quantify the reagents needed for the remediation. For the execution phase the following application methods were performed and analysed: reagents injection into monitoring wells, injection by direct push into the soil, injection into devoted injection wells, application of the reagents into filter socks, application of the reagents into a dig. Pros and cons of each method were so identified. During the monitoring phase the remediation efficacy was evaluated using, not only chemical concentration of contaminants, but also chemicalphysical parameters spatial distribution as a valid support. In the wide range of remediation technologies, the use of chemical reagents has been progressively more appreciated by the designers and by public authorities and is now extensively applied because it is in some cases more effective, cheaper or quicker. These technologies, in fact, proved to be a solid and sustainable alternative to plant technologies, and they were very effective also in fine aquifers. For 62% of the analysed sites, chemical injections led to a reduction of the contamination within 1 year from the application.

The effect of bioaugmentation with Exiguobacterium sp. AO-11 on crude oil removal and the bacterial community in sediment microcosms, and the development of a liquid ready-to-use inoculum

This study demonstrates the feasibility of using Exiguobacterium sp. AO-11 to remediate oil-contaminated environments. Bioaugmentation using AO-11 showed the best removal percentage, 75%, of 4% (w/w) crude oil in sediment microcosms in 100 days. In terms of the bacterial community structure during crude oil degradation, the addition of AO-11 did not change the indigenous bacterial community, while the addition of urea fertilizer induced structural shift of indigenous bacterial community. Exiguobacterium sp. AO-11 was developed as a bioremediation product, and a liquid formulation of AO-11 was developed. Coconut milk residue and soybean oil mill sludge were used for bacterial cultivation to reduce the production cost, and they could enhance bacterial cell growth. The liquid formulation of AO-11 prepared in phosphate buffer could be stored at 4 °C for at least 2 months, and it maintained efficacy in the treatment of crude oil-contaminated seawater. Overall, bioaugmentation with strain AO-11 could be an effective solution for the bioremediation of crude oil-contaminated environments.

Silk: A Promising Biomaterial Opening New Vistas Towards Affordable Healthcare Solutions

Substantial progress in biomaterial research over the years has culminated in revolutionary technological advancements in the healthcare domain. This has triggered the quest for affordable healthcare solutions with focus on sustainable biomaterials with versatile applications endowed with green fabrication strategies. Silk as a biopolymer has garnered special attention which can largely be attributed to the excellent material properties of silk in addition to its affordability and resource ability. Silk fibroin from various silkworm and spider species and sericin from various silkworm species have been researched for their potential applications in the healthcare industry such as tissue-engineered grafts, cancer therapeutics, high-throughput tissue-on-chip models, food preservatives, biomedical imaging, biosensing, biomedical textiles, implants, cosmetics and bioremediation products. The present review mainly focusses on the various sources of silk fibroin and its relevant properties that have been conferred to it by nature. Moreover, recent developments, progress and prevalent modalities of healthcare industry that involve the application of silk fibroin and sericin have been outlined in the present review.

Allochthonous Diesel Biodegradation by Bacillus sp. MO1 Isolated from Diesel-contaminated Soil

Ten bacterial isolates were successfully isolated from pristine and contaminated areas in Malaysia. There was no lag phase observed signifying each of these isolates was able to quickly induce cellular machinery for diesel assimilation. The best isolate was tentatively identified as Bacillus sp. strain MO1 based on carbon utilisation profiles using Biolog GP plates and partial 16S rDNA matching. Strain MO1 grew optimally at 37 oC on 4% (v/v) diesel, pH 7.5 and 0.9% (w/v) KNO3. Diesel components were proven to be completely eliminated from the reduction in the hydrocarbon peaks monitored by gas chromatographic analysis after 6 days of incubation. The bacterium was found to be a better remediation agent than a commercial bioremediation product. All of these characteristics suggest that this bacterium is the suitable bacterium for bioremediation of diesel spills and pollution in the tropics.

Development of peroxidase enzyme immobilized magnetic nanoparticles for bioremediation of textile wastewater dye

Peroxidase enzyme plays a major role in the degradation of phenolic compounds such as azo dyes. This enzyme was isolated and purified from the bioremediation products of textile wastewater containing direct green azo dye in a UFC/CSA prototype bioreactor. To improve the enzyme stability, efficiency and recyclability, peroxidase was immobilized on Fe3O4 magnetic nanoparticles produced and modified by co-precipitation using glutaraldehyde. The immobilized enzyme was characterized by transmission electron microscopy analysis and examined against different pH as well as storage time and temperatures. The MNPs-immobilized peroxidise was remarkable stable towards temperature and pH perturbations, compared to the free enzyme form. Moreover, the immobilized peroxidase maintained its full activity upon storage at 4 and 25 °C for 90 days, and upon recycling for up to 100 cycles. Finally, the MNPs-immobilized peroxidase was successfully employed to decolorize dyes of different chemical natures in a lab scale bioreactor. Hence, we believe that the immobilized peroxidase on magnetic nanoparticles reported in this work can be potentially used in various industrial and environmental applications.

Correlative Cryogenic Spectromicroscopy to Investigate Selenium Bioreduction Products.

Accurate mapping of the composition and structure of minerals and associated biological materials is critical in geomicrobiology and environmental research. Here, we have developed an apparatus that allows the correlation of cryogenic transmission electron microscopy (cryo-TEM) and synchrotron hard X-ray microprobe (SHXM) data sets to precisely determine the distribution, valence state, and structure of selenium in biofilms sampled from a contaminated aquifer near Rifle, CO. Results were replicated in the laboratory via anaerobic selenate-reducing enrichment cultures. 16S rRNA analyses of field-derived biofilm indicated the dominance of Betaproteobacteria from the Comamonadaceae family and uncultivated members of the Simplicispira genus. The major product in field and culture-derived biofilms is ∼25-300 nm red amorphous Se0 aggregates of colloidal nanoparticles. Correlative analyses of the cultures provided direct evidence for the microbial dissimilatory reduction of Se(VI) to Se(IV) to Se0. Extended X-ray absorption fine-structure spectroscopy showed red amorphous Se0 with a first shell Se-Se interatomic distance of 2.339 ± 0.003 Å. Complementary scanning transmission X-ray microscopy revealed that these aggregates are strongly associated with a protein-rich biofilm matrix. These findings have important implications for predicting the stability and mobility of Se bioremediation products and understanding of Se biogeochemical cycling. The approach, involving the correlation of cryo-SHXM and cryo-TEM data sets from the same specimen area, is broadly applicable to biological and environmental samples.

Composition and pathogenic potential of a microbial bioremediation product used for crude oil degradation.

A microbial bioremediation product (MBP) used for large-scale oil degradation was investigated for microbial constituents and possible pathogenicity. Aerobic growth on various media yielded >108 colonies mL-1. Full-length 16S rDNA sequencing and fatty acid profiling from morphologically distinct colonies revealed ≥13 distinct genera. Full-length 16S rDNA library sequencing, by either Sanger or long-read PacBio technology, suggested that up to 21% of the MBP was composed of Arcobacter. Other high abundance microbial constituents (>6%) included the genera Proteus, Enterococcus, Dysgonomonas and several genera in the order Bacteroidales. The MBP was most susceptible to ciprofloxacin, doxycycline, gentamicin, and meropenam. MBP exposure of human HT29 and A549 cells caused significant cytotoxicity, and bacterial growth and adherence. An acellular MBP filtrate was also cytotoxic to HT29, but not A549. Both MBP and filtrate exposures elevated the neutrophil chemoattractant IL-8. In endotracheal murine exposures, bacterial pulmonary clearance was complete after one-week. Elevation of pro-inflammatory cytokines IL-1β, IL-6, and TNF-α, and chemokines KC and MCP-1 occurred between 2h and 48h post-exposure, followed by restoration to baseline levels at 96h. Cytokine/chemokine signalling was accompanied by elevated blood neutrophils and monocytes at 4h and 48h, respectively. Peripheral acute phase response markers were maximal at 24h. All indicators examined returned to baseline values by 168h. In contrast to HT29, but similar to A549 observations, MBP filtrate did not induce significant murine effects with the indicators examined. The results demonstrated the potentially complex nature of MBPs and transient immunological effects during exposure. Products containing microbes should be scrutinized for pathogenic components and subjected to characterisation and quality validation prior to commercial release.

Characterization of Chromium Bioremediation Products in Flow-Through Column Sediments Using Micro-X-ray Fluorescence and X-ray Absorption Spectroscopy.

Microbially mediated reductive immobilization of chromium is a possible remediation technique for sites contaminated with Cr(VI). This study is part of a broader effort investigating the biogeochemical mechanisms for Cr(VI) reduction in Hanford 100H aquifer sediments using flow-through laboratory columns. It had previously been shown that reduced chromium in the solid phase was in the form of freshly precipitated mixed-phase Cr(III)-Fe(III) (hydr)oxides, irrespective of the biogeochemical conditions in the columns. In this study, the reduced Cr phases in the columns were investigated further using spectroscopy to understand the structure and mechanisms involved in the formation of the end products. Several samples representing potential processes that could be occurring in the columns were synthesized in the laboratory and characterized using X-ray absorption near edge structure (XANES) and X-ray scattering. The XANES of Cr(III) particles in the columns most closely resembled those from synthetic samples produced by the abiotic reaction of Cr(VI) with microbially reduced Fe(II). Microbially mediated Cr-Fe reduction products were distinct from abiotic Cr-Fe (hydr)oxides [CrFe(OH)] and organically complexed Cr(III) sorbed onto the surface of a mixed ferrihydrite-goethite mineral phase. Furthermore, analyses of the abiotically synthesized samples revealed that even the end products of purely abiotic, iron-mediated reduction of Cr(VI) are affected by factors such as the presence of excess aqueous Fe(II) and cellular matter. These results suggest that CrFe(OH) phases made under realistic subsurface conditions or in biotic cultures are structurally different from pure Cr(OH) or laboratory-synthesized CrFe(OH). The observed structural differences imply that the reactivity and stability of biogenic CrFe(OH) could potentially be different from that of abiotic CrFe(OH).

Environmental Microbiology and Biotechnology: Progress and Prospects

AbstractEnvironmental microbiology and biotechnology are crucial in the modern society. They influence human health, environment and serve as a source of energy and provider of novel bioproducts. The key areas of interest are bioremediation, microbial communities, pathogen detection, wastewater treatment, energy generation, bioprocesses, and natural product discovery. Environmental microbiology must interact with hydrology, biogeochemistry, limnology, oceanography and numerical modeling. This subject will continue to engage future generations of scientists, engineers, entrepreneurs and policy makers.

Starting study of microbial genetics diversity in soils of mangrove preserved in sergipe

Tropical mangroves are considered one of the most productive ecosystems in the world, characterized by high rate of cycling of organic matter and nutrients that occurs between the oceans and terrestrial environments. This paper proposes the characterization and evaluation of structures and diversity of microbial communities in the soil of mangroves in three conservation areas located in Sergipe. The samples were analyzed by the extraction, purification and analysis molecucar. Soil samples were collected in PVC tubes 50 cm long and 50 mm in diameter, placed vertically in the sediment of three different regions of the state of Sergipe, in triplicate. For the extraction of total DNA from soil was used Power Soil DNA Kit, an aliquot of 5 µl of the extracted DNA was subjected to electrophoresis on agarose gel 1 % (w/v) stained with ethidium bromide (0,5 mg/ml gel) in TAE buffer. Was used as standard molecular 2 µl of Low Mass DNA Ladder. The gel was subjected to an electric field of 80 V for approximately 30 min and then photo-documented. The extracted DNA was also quantified by spectrophotometer. For purification of PCR products was used Kit GFXTM PCR DNA and Gel Band Purification by following the manufacturer's directions and visualized on agarose gels and subjected to an electric field of 80 V for approximately 30 min. With tags generated by using a primer specific 16S constructed a data matrix and calculated the diversity of the samples, using the Jaccard coefficient and UPGMA. The results were generated from the software FreeTree by which a dendrogram was obtained. The genotypes were grouped into three groups among the samples, group I: genotype accesses of the region 1, group II: genotype accesses of region 2, and group III: visualize genotype accesses of the region 3, checking the genetic distance between accesses collected from the regions. The high degree of polymorphism identified among samples represents heterozygosity for this marker, which can contribute to infer genetic relationships. Such diversity will in comparison studies of the diversity present in genomic databases. Thus, we conclude that there is diversity among the genotypes of the studied samples. The 16S rRNA gene has been used extensively in phylogenetic inference of microorganisms, however, because it is an initial study is still in the process of identification the total sequences of mangroves to continue with studies analyzing sequences isolated. In an attempt to attribute function to these genes and then use the potential of lineages. Observing this, we realize that the genotypes showed diversity among the samples collected. Such diversity will in comparison studies of the diversity present in genomic databases. Thus, we conclude that there is diversity among the genotypes of the studied samples. These results allow to conclude that the mangrove has exclusive characteristics, besides contributing to initial information on the study, to select genotypes that may be possible potential in bioremediation and other biotechnological products and industrial to be explored from the knowledge that diversity.