Biomonitoring Of Trace Elements Research Articles

Macroalgae for biomonitoring of trace elements in relation to environmental parameters and seasonality in a sub-tropical mangrove estuary

Being a resourceful ecosystem, mangrove estuaries have always been subjected to trace elements (TEs) contamination, and therefore, the biomonitoring approach holds immense potential for surveilling the aquatic environment. To investigate the potentiality of mangrove macroalgae as biomonitors, estuarine water, intertidal-sediment, and macroalgal samples were collected from the Pasur River estuary of Sundarbans mangrove ecosystem, Bangladesh, and afterward studied through Atomic Absorption Spectrometer to quantify the levels of six concerned TEs (Fe, Mn, Zn, Cu, Pb, and Cd). This study utilized the geo-environmental and ecological indices and sediment characterization approaches (sediment quality guidelines-SQGs) for assessing the contamination scenario of the adjacent environment to macroalgae whereas the performance of studied algal groups was evaluated using Bio-contamination factor, Comprehensive bio-concentration index, and Metal accumulation index. Metal occurrence scheme in the water followed the order of Fe > Zn > Mn > Pb > Cd while Fe > Mn > Zn > Cu > Pb > Cd for both sediment and macroalgae. Both Pb and Cd exceeded the guideline limit in estuarine water and the indices approach manifested low to moderate contamination with enrichment from anthropogenic origin of Mn, Zn, and Cu in sediment. Moreover, the SQGs revealed rare biological effects of Cu on an aquatic community. Within algal samples, Chlorophyta contributed the highest biomass production, followed by Phaeophyta and Rhodophyta. Statistical relationship disclosed the influence of environmental variables on TE's accumulation in Chlorophyta. By contrast, hydrochemical's association showed prevalence over the TEs accumulation process for Phaeophyta and Rhodophyta. Bioaccumulation performance analysis revealed that the ability to accumulate TEs in macroalgal groups varied with seasons. Therefore, biomonitoring with macroalgae for the region of interest might require further temporal considerations.

Active and passive biomonitoring of trace elements, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in small Mediterranean harbours

Pollution particularly affects coastal ecosystems due to their proximity to anthropic sources. Among those environments, harbours are subjected to marine traffic but also to accidental and chronic pollution. These areas are thus exposed to complex mixtures of contaminants such as trace elements and organic contaminants which can impact marine species, habitats, and ecosystem services. The monitoring of these compounds is thus a crucial issue for assessment of environmental health. In this context, the aim of the present work was to evaluate the chemical contamination of harbours in Corsica (NW Mediterranean) by measuring the bioaccumulation of trace elements, polycyclic aromatic hydrocarbons, and polychlorinated biphenyls in mussels, limpets, and sea cucumbers. The human health risks associated with seafood consumption were also assessed. Results reveal a relatively low contamination in the Corsican harbours studied compared to larger Mediterranean ports and suggest that the potential health risk for consumers eating seafood is low.

Tissue-based trace element pollution of clam Ruditapes philippinarum in China: Hotspot identification and multiple nonlinear analysis

Considering the complexity of coastal and estuarine systems, a great challenge of environmental health assessment is to distinguish between natural and anthropogenically induced stress. Quantification of trace element accumulation in the tissues of sedentary bivalves with subsequent hotspot identification is important to assess the pollution status. The present study conducted a nationwide mapping of bioavailable macro- and trace elements in a widely distributed biomonitoring clam Ruditapes philippinarum from China. Ag, As, Cd, Cr, Cu, and Zn concentrations in the clams showed similar levels as those documented previously in mussels, but were lower than those in oysters at similar sites from China. Notably, the total As concentrations in clams at Xinkai Estuary and Beibu Bay were relatively higher than those at other sites in China. After normalization by tissue biomass, salinity (Na) and nutrient (P), some hotspots were identified with high pollution of trace elements at Liaodong Bay of Bohai Sea, Gold Beach of Qingdao, Dongling Port of Yellow Sea, Hangzhou Bay and adjacent coasts of East China Sea, and Pearl River Estuary and Beibu Bay of South China Sea. This study demonstrated that most trace elements had a path-dependent effect of biomass, except for Cd which showed an indirect pathway of AgNi related accumulation. Results showed significant correlations between Cd, Zn, Ag and Ni, and between Pb/Cr and Ti in clams. After mass normalization, all trace elements displayed significantly positive correlations with Na or P. Simultaneously, the clam biomass played an intermediary role in trace element accumulation in non-linear patterns related to salinity and nutrient. These results are important in evaluating the composite ambiguous information of the historical data of trace element biomonitoring.

Human Biomonitoring of Trace Elements (Zn, Cu, Mn, Cd and As) Level in a Healthy Urban Population in Türkiye

Human Biomonitoring of Trace Elements (Zn, Cu, Mn, Cd and As) Level in a Healthy Urban Population in Türkiye

The relatively higher concentrations of trace elements to arsenic may have influenced on methylation process of inorg- As in humans

The relatively higher concentrations of trace elements to arsenic may have influenced on methylation process of inorg- As in humans

First Results on Moss Biomonitoring of Trace Elements in the Central Part of Georgia, Caucasus

The moss biomonitoring technique was used for assessment of air pollution in the central part of Georgia, Caucasus, in the framework of the UNECE ICP Vegetation. A total of 35 major and trace elements were determined by two complementary analytical techniques, epithermal neutron activation analysis (Na, Mg, Al, Cl, K, Ca, Sc, Ti, V, Cr, Mn, Fe, Co, Ni, Zn, Se, B, Rb, Sr, Zr, Mo, Sb, I, Cs, Ba, La, Ce, Nd, Sm, Eu, Tb, Yb, Hf, Ta, W, Th, and U) and atomic absorption spectrometry (Cu, Cd, and Pb) in the moss samples collected in 2019. Principal Component Analyses was applied to show the association between the elements in the study area. Four factors were determined, of which two are of geogenic origin (Factor 1 including Na, Al, Sc, Ti, V, Cr, Fe, Co, Ni, Th, and U and Factor 3 with As, Sb, and W), mixed geogenic–anthropogenic (Factor 2 with Cl, K, Zn, Se, Br, I, and Cu) and anthropogenic (Factor 4 comprising Ca, Cd, Pb, and Br). Geographic information system (GIS) technologies were used to construct distributions maps of factor scores over the investigated territory. Comparison of the median values with the analogous data of moss biomonitoring in countries with similar climatic conditions was carried out.

Walrus teeth as biomonitors of trace elements in Arctic marine ecosystems

Effective biomonitoring requires an understanding of the factors driving concentrations of the substances or compounds of interest in the tissues of studied organisms. Biomonitoring of trace elements, and heavy metals in particular, has been the focus of much research; however, the complex roles many trace elements play in animal and plant tissues can make it difficult to disentangle environmental signals from physiology. This study examined the concentrations of 15 trace elements in the teeth of 122 Pacific walruses (Odobenus rosmarus divergens) to investigate the potential for walrus teeth as biomonitors of trace elements in Arctic ecosystems. Elemental concentrations were measured across cementum growth layer groups (GLGs), thereby reconstructing a lifetime history of element concentrations for each walrus. The locations of GLGs were used to divide trace element time series into individual years, allowing each GLG to be associated with an animal age and a calendar year. The elements studied exhibited a great deal of complexity, reflecting the numerous factors responsible for generating tooth trace element concentrations. Generalized linear mixed models were used to investigate the importance of age and sex in explaining observed variation in trace element concentrations. Some elements exhibited clear physiological signals (particularly zinc, strontium, barium, and lead), and all elements except arsenic varied by age and/or sex. Pearson's correlations revealed that elements were more strongly correlated among calendar years than among individual walruses, and correlations of trace elements within individual walruses were generally inconsistent or weak. Plots of average elemental concentrations through time from 1945 to 2014 further supported the correlation analyses, with many elements exhibiting similar patterns across the ~70-year period. Together, these results indicate the importance of physiology in modulating tooth trace element concentrations in walrus tooth cementum, but suggest that many trace elements reflect a record of environmental exposure and dietary intake/uptake.

Influence of secondary metabolites on surface chemistry and metal adsorption of a devitalized lichen biomonitor.

Despite the broad use of lichens as biomonitors of airborne trace elements, the surface chemistry and metal adsorption parameters of these organisms are still poorly known. The current investigation is aimed at (i) quantifying the acid-base surface properties and the first-order physical-chemical parameters of Cu2+ and Zn2+ adsorption of devitalized Pseudevernia furfuracea, a lichen commonly used in biomonitoring of airborne trace elements, and (ii) comparing the results with those available for moss biomonitors. Equilibrium constants and metal-binding site concentrations were calculated with a thermodynamic model by taking into account the presence/absence of ancillary extracellular cell wall compounds, namely melanin and acetone-soluble lichen substances. An acid-base titration experiment performed in the pH range of 3-10 showed that melanised and non-melanised P.furfuracea samples have lower pHPZC (3.53-3.99) and higher metal-binding site concentrations (0.96-1.20mmolg-1) compared to that of the mosses investigated so far at the same experimental conditions. Melanin biosynthesis increased the content of carboxyl and phosphoryl groups and reduces that of amine/polyphenols. Cu2+ and Zn2+ adsorption was unaffected by the degree of melanisation while the removal of extracellular lichen substances slightly decreased Zn2+ adsorption. Although Cu2+ and Zn2+ adsorption parameters related to P.furfuracea surfaces were 3 times lower than in the mosses, lichen samples adsorbed the same amount of Cu2+ and 30% more Zn2+. The present study contributes in understanding the role of ancillary cell wall compounds in Cu2+ and Zn2+ adsorption in a model lichen. It also provides a first comparison between the surface physico-chemical characteristics of lichens and mosses frequently used as biomonitors of trace elements.

Seagrass Cymodocea nodosa and seaweed Ulva lactuca as tools for trace element biomonitoring. A comparative study

This study compared the capacity of the seagrass Cymodocea nodosa and the green alga Ulva lactuca to sequester the trace elements Cd, Cr, Cu, Ni, Pb and Zn, in seawater and benthic sediments, under the same environmental conditions. Samples of C. nodosa leaves and U. lactuca thalli were collected in four sites off the coast of Sicily (Italy), with varying degrees of anthropogenic impact. C. nodosa accumulated generally higher levels of trace elements, suggesting a better performance in marine phytoremediation. In turn, C. nodosa and U. lactuca showed a similar performance regarding the bioindication of trace elements. Since salinity, temperature and pH had relatively constant values, their role in the accumulation processes in both species should be considered as secondary. Although C. nodosa and U. lactuca fulfill several criteria as bioindicators, including widespread distribution, abundance and tolerance to toxicity, their regular use in biomonitoring programs is still limited.

Air quality biomonitoring of trace elements in the metropolitan area of Huancayo, Peru using transplanted Tillandsia capillaris as a biomonitor

The air quality and distribution of trace elements in a metropolitan area of the Peruvian Andes were evaluated using transplanted epiphytic Tillandsia capillaris as biomonitors. Biomonitors were collected from the non-contaminated area and exposed to five sites with different types of contamination for three months in 2017. After exposure, the content of twenty-one elements were determined by ICP-MS analysis. Datasets were evaluated by one-way ANOVA, exposed-to-baseline (EB), hierarchical cluster analysis (HCA) and principal component analysis (PCA). Results showed significant differences among sampling sites for several elements. According to EF ratios for Ba, Cr, Cu, Pb, Sb, and Zn EB ratios value greater than 1.75 were found around urban areas, indicating anthropogenic influence, which can be attributed to vehicular sources. The highest values of As and Cd were found in areas of agricultural practices, therefore their presence could be related to the employment of agrochemicals (pesticides, herbicides, and phosphate fertilizers). HCA shows that most elements come from vehicular sources and lower from agricultural and natural sources.

Human Biomonitoring of Trace Elements in Scalp Hair from Healthy Population of Pakistan.

The present study deals with the assessment of essential trace and toxic elements in biological samples (scalp hair) of healthy smoker and non-smoker residents of Lahore (the second most populous city), Pakistan. Human biomonitoring of various trace elements like zinc (Zn), iron (Fe), copper (Cu), chromium (Cr), cadmium (Cd), nickel (Ni), lead (Pb), and arsenic (As) was evaluated in human hair samples of smokers and non-smokers (males and females) as per IFCC criteria. For the purpose of comparison, scalp hair samples of age- and sex-equivalent healthy persons were also analyzed. The results of this study show that the concentrations (P95 values) of As (0.17, 0.81, and 0.91μg/g), Cd (2.80, 3.81, and 3.16μg/g), Cr (4.1, 4.2, and 5.3μg/g), Cu (20.0, 21.0, and 21.9μg/g), Ni (3.9, 4.6, and 4.3μg/g), Pb (4.0, 4.8, and 5.0μg/g), and Fe (49.0, 49.0, and 59.3μg/g) were significantly higher in scalp hair samples of smokers than in referents, for various age groups (16-32years, 33-50years, and 51-62years), respectively. The concentrations of Zn (165, 163, and 173μg/g various age groups, respectively) in similar age group were found lower in the scalp hair samples of smokers than referents. Exposure of high toxic metal level through smoking and deficiency of Zn could be synergistic with the risk factors associated with the use of tobacco. Correlation studies for the elements in cigarette with the elements in scalp hair of male smokers show a highly positive correlation between Cr-Cd, Cr-Ni, Cu-Fe, and Ni-Zn.

Trace elements in saliva and plasma of patients with type 2 diabetes: Association to metabolic control and complications

AimAn analysis is made of the saliva and plasma levels of trace elements in patients with type 2 diabetes mellitus and their association to metabolic control and the presence of chronic complications. MethodsA cross-sectional observational clinical study was carried out in 74 patients with type 2 diabetes mellitus. Mass spectrometry (ICP-MS) was used to determine the following trace elements in plasma and unstimulated basal saliva: 13Al, 16S, 4Be, 5B, 20Ca, 27Co, 29Cu, 24Cr, 38Sr, 15P, 3Li, 12Mg, 25Mn, 28Ni, 82Pb, 37Rb, 22Ti, 23V and 30Zn. ResultsThe levels of cobalt (p = 0.048) in saliva and of strontium (p = 0.001) in plasma were related to the presence of chronic complications. Significant differences with respect to metabolic control were observed for beryllium (p = 0.038), boron (p = 0.023) and phosphorus in saliva (p = 0.046), and for rubidium (p = 0.005), titanium (p = 0.016) and zinc in plasma (p = 0.013). A significant correlation (p < 0.001) was found between boron in plasma and boron in unstimulated basal saliva. ConclusionsThe determination of trace elements in plasma and saliva constitutes a complementary tool for the assessment of metabolic control and for predicting chronic complications associated to type 2 diabetes mellitus. Further studies involving the biomonitoring of trace elements in saliva and plasma are needed.

A Study of the consideration of bio monitoring of trace Elements in the human nail for the Biometric Authentication

the studies of trace element composition in the human nail is called ashuman bio monitoring. The composition of the chemicals and traceelements and concentration of the chemicals and trace elements in thehuman body fluids and human body tissues are observed .Theconcentration of trace elements for a healthy human being is stable for aparticular period of time in their nail and also the composition of chemicalelements in the nail differs to each individual. So the humans nails can beused as the bio marker for monitoring or quantifying the composition ofchemicals (major and trace elements) in the human body. So traceelement's composition and concentrations in the nail can be considered forBio-metrics authentication in future. Because the chemical composition ofnail and chemical concentration in the human nail differ from one peopleto another people and so this can be used to identify the individual infuture.Index terms–elements, trace elements, human bio monitoring, humanbody, nail

Trace element biomonitoring in hair and blood of occupationally unexposed population residing in polluted areas of East Kazakhstan and Pavlodar regions

IntroductionEastern and North-Eastern regions of Kazakhstan are considered to be environmentally disadvantaged due to industrial pollution and activity of the former Semipalatinsk Nuclear Test Site. Ferrous metallurgy is represented by the world’s largest ferroalloy plant located in Aksu. In addition to a ferroalloy plant, Aksu is the home for the largest thermal power plant in Kazakhstan. ObjectiveBiomonitoring of 31 hair and blood trace elements (Ag, Ba, Be, Bi, Cs, Co, Ce, Cr, Cu, Eu, Gd, Hf, In, La, Li, Mn, Mo, Nb, Nd, Pb, Sc, Sn, Tl, Th, U, V, W, Y, Yb, Zn, and Zr) in non-occupationally exposed population residing in polluted areas of East Kazakhstan and Pavlodar regions. MethodsFive case groups, residing in the vicinity to the former Semipalatinsk Nuclear Test Site (Akzhar, Borodulikha, and Karaul) or in proximity to industrial plants (Aksu and Ust-Kamenogorsk) have been assessed vs. controls from a rural settlement in Kurchum. In total, 204 hair and blood samples were analyzed by inductively coupled plasma mass spectrometry. ResultsThe observed blood concentrations of trace elements were in agreement with earlier studies on residents of industrially polluted areas. Elevated levels of blood Ba, Mn, Pb, V, and Zn were detected in residents of Aksu and Ust-Kamenogorsk. The elemental composition of head hair was characterized by greater stability between the study sites. ConclusionResidency near the former Semipalatinsk Test Site could be considered as safe, while the environmental status of industrial settlements appears to be rather adverse.

Comparison Between Two Submerged Macrophytes as Biomonitors of Trace Elements Related to Anthropogenic Activities in the Ctalamochita River, Argentina.

The temporal variation of As, Co, Cr, Cu, Fe, Mn, Ni, Pb, V and Zn in surface waters and sediments, and trace element accumulation and physiological changes in the macrophytes Stuckenia filiformis and Potamogeton pusillus, were estimated in the Ctalamochita River, Argentina, both upstream and downstream of Río Tercero. Chromium, Fe, Pb and Zn in surface water were higher at the downstream site, while Cu and Mn were higher upstream. Chromium, Mn and Zn in S. filiformis correlated with concentrations observed in sediments, whereas only Zn did in water. In P. pusillus, As, Co, Cr, Ni, Pb and Zn correlated with concentrations in sediments. P. pusillus revealed greater variations in the photosynthetic pigments and malondialdehyde content in the site downstream of the city than those observed in S. filiformis. Therefore, P. pusillus has a greater potential use in monitoring studies in aquatic environments with ecological risk than S. filiformis.

Urban background of air pollution: Evaluation through moss bag biomonitoring of trace elements in Botanical garden

Urban background air pollution is the lowest level of pollution representative for the exposure of general urban population and mainly originates from non-local sources of pollution. Moss bag technique has been predominantly adopted for biomonitoring of trace elements across anthropogenically devastated areas, such as urban and industrial zones. However, the technique has been rarely used for measurement of background air pollution. In this study, element content and concomitant physiological parameters were assessed in the moss Hypnum cupressiforme Hedw. after its exposure within the bags in the Botanical garden (Belgrade, Serbia), as the presumable background area. During the summer of 2014, the moss bags were exposed for 60days in total, and the sample analyses were performed every 15days. As a control of the measured physiological parameters, a set of the moss bags was kept within the phytotron, under conditions of optimal-like growth. The total content of 21 elements, photosynthetic pigments (chlorophylls and carotenoids), phenolics, and antioxidative capacity were determined in the moss samples. The levels of trace elements were markedly lower within the Botanical garden in comparison to the street ambient, but still significantly higher than in the moss pristine habitat. However, the performed physiological tests indicated that the vulnerability of the moss during exposure time was not caused by the low trace element enrichment, but probably by the harsh meteorological parameters in an urban area. Finally, the Botanical garden could be assumed as control site for measurement of the urban background pollution, and it might be effectively measured by the moss bag technique.

Trace element biomonitoring in hair of school children from a polluted area by sector field inductively coupled plasma mass spectrometry

In the current study, a biomonitoring of 18 hair trace elements (Al, As, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Ni, Pb, Se, V, Zn, Ca, Na and P) in school children from Leningradskaya Oblast’ is reported. A case group, residing in a proximity to the toxic waste disposal grounds (Krasniy Bor), has been assessed vs. controls from a non-urban settlement Seltso. In total, 166 hair samples were analysed using double focusing sector field inductively coupled plasma mass spectrometry after microwave-assisted sample digestion with nitric acid. For the determination of Ca, Na and P inductively coupled plasma optical emission spectrometry was employed. For the validation, a reference material and spiked hair samples were analysed. The data obtained was processed using parametric statistics and factor analysis. Determined concentrations of trace elements were in agreement with the previously published results on chemically polluted areas. In the case group, linear correlations between Al, Cr, Cu, Fe, Ni and V were observed. Also, these metals correlated to selenium hair content in the case group. Additionally, a correlation between hair Se and P was observed in the case subjects. Several gender differences in trace content were observed within each group. However, no age- or body index-related difference was found. The obtained results show that closely located waste disposal grounds intensifies trace element exposure in school children of Krasniy Bor. However, judging from rather high values for the controls, total environmental status of the region seems to be unstable, so additional monitoring and chemical safety measures are required.

Metal and Microelement Biomarkers of Neurodegeneration in Early Life Permethrin-Treated Rats.

Hair is a non-invasive biological material useful in the biomonitoring of trace elements because it is a vehicle for substance excretion from the body, and it permits evaluating long-term metal exposure. Here, hair from an animal model of neurodegeneration, induced by early life permethrin treatment from the sixth to 21th day of life, has been analyzed with the aim to assess if metal and microelement content could be used as biomarkers. A hair trace element assay was performed by the ICP-MS technique in six- and 12-month-old rats. A significant increase of As, Mg, S and Zn was measured in the permethrin-treated group at 12 months compared to six months, while Si and Cu/Zn were decreased. K, Cu/Zn and S were increased in the treated group compared to age-matched controls at six and 12 months, respectively. Cr significantly decreased in the treated group at 12 months. PCA analysis showed both a best difference between treated and age-matched control groups at six months. The present findings support the evidence that the Cu/Zn ratio and K, measured at six months, are the best biomarkers for neurodegeneration. This study supports the use of hair analysis to identify biomarkers of neurodegeneration induced by early life permethrin pesticide exposure.

Analyzing the effect of atmospheric trace elements on selected plant species

Foliar accumulation of trace elements (TE) has gained great ecological importance in recent times. The current study tries to evaluate two naturalized common plant species, Pongamia pinnata (Karanj) and Kigelia africana (sausage tree) as biomonitors of trace elements by calculating their trapping efficiency for nine heavy metals—Pb, Fe, Zn, Mn, Ni, Cd, Cr, Co and Cu, at two different land use sites: Site I, vegetated area and Site II, commercial area (polluted). The effect of atmospheric TE was examined by analyzing the plasma membrane intactness and total chlorophyll content of selected plant species. The result depicted that Pongamia sp. is a better trapper of heavy metal (Site I: 0.407 ppm/g leaf sample and Site II: 1.174 ppm/g leaf) as compared to Kigelia sp. (Site I: 0.302 ppm/g leaf and Site II: 0.661 ppm/g leaf). Moreover, leaf samples of both the plants showed high TE pollution at Site II as compared to Site I. The disruption of plasma membrane was more at commercial area (Site II) as compared to vegetated area (Site I) in both the plant samples, with leakage percentage of 48.1 % (Site I) and 65.6 % (Site II) in Pongamia pinnata and 69.47 % (Site I) and 77.51 % (Site II) in Kigelia africana, respectively. The decreased chlorophyll content at Site II (Karanj 1.62 mg/g and sausage 1.23 mg/g) as compared to Site I (Karanj 2.21 mg/g and sausage 1.35 mg/g) further supported the negative effect of the polluted environment of commercial area (Site II). The study clearly illustrates that plant leaf samples can act as efficient biomonitors and implicitly tell about the health of the surrounding environment. However, evaluation and use of biomonitors still needs lot of study for better understanding of complex factors that influence their responses.

Possibilities of using deciduous tree species in trace element biomonitoring in an urban area (Plovdiv, Bulgaria)

Leaves of Acer platanoides L., Aesculus hippocastanum L. and Betula pendula Roth. were collected from urban sites with different anthropogenic impact in the city of Plovdiv (Bulgaria). Concentrations of Cd, Cr, Cu, Fe, Pb and Zn in foliage samples were analyzed by ICP–MS. Three studied deciduous trees clearly showed variations in element concentrations depending on the sampling site (Pb, Fe, Cr) and the plant species (Zn). Betula pendula was found to be a better bioaccumulator for the elements Cr, Cd, and especially for Zn. Our approach could be successfully applied in the monitoring of air pollution due to trace elements in urban areas, regardless of their size and topography.